[M::bwa_idx_load_from_disk] read 3171 ALT contigs [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 662202, 51, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (126, 666) [M::mem_pestat] mean and std.dev: (397.22, 82.70) [M::mem_pestat] low and high boundaries for proper pairs: (18, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 33, 58) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 140) [M::mem_pestat] mean and std.dev: (33.21, 27.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 181) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1172.660 CPU sec, 67.804 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 661815, 44, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.00, 82.83) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 45, 647) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1907) [M::mem_pestat] mean and std.dev: (192.55, 390.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2537) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1158.817 CPU sec, 51.347 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 661440, 49, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.19, 82.72) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 29, 125) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 337) [M::mem_pestat] mean and std.dev: (36.23, 48.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 443) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1151.640 CPU sec, 64.540 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 662422, 51, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (198, 353, 3234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9306) [M::mem_pestat] mean and std.dev: (1935.90, 2374.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12342) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.08, 82.77) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 33, 708) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2098) [M::mem_pestat] mean and std.dev: (147.36, 383.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2793) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1147.654 CPU sec, 50.654 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 662449, 56, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (49, 315, 1827) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5383) [M::mem_pestat] mean and std.dev: (794.20, 1008.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7161) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (396.93, 82.86) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 43, 108) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 282) [M::mem_pestat] mean and std.dev: (45.65, 41.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 369) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1138.503 CPU sec, 63.030 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 661762, 45, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (223, 345, 411) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 787) [M::mem_pestat] mean and std.dev: (324.21, 117.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 975) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.19, 82.71) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 79, 2627) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7809) [M::mem_pestat] mean and std.dev: (1107.00, 1661.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10400) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1140.321 CPU sec, 50.411 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 661807, 57, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (164, 326, 1275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3497) [M::mem_pestat] mean and std.dev: (730.36, 906.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4608) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.13, 82.87) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 28, 101) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 271) [M::mem_pestat] mean and std.dev: (33.85, 34.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 356) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1156.304 CPU sec, 68.029 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 661754, 55, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (126, 666) [M::mem_pestat] mean and std.dev: (397.18, 82.58) [M::mem_pestat] low and high boundaries for proper pairs: (18, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 23, 66) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 162) [M::mem_pestat] mean and std.dev: (25.79, 21.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 210) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1143.351 CPU sec, 50.622 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 662172, 54, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (322, 425, 457) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (52, 727) [M::mem_pestat] mean and std.dev: (324.12, 126.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 862) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.04, 82.75) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 38, 1077) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3199) [M::mem_pestat] mean and std.dev: (353.51, 679.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4260) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1154.384 CPU sec, 63.952 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 662149, 51, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (291, 342, 1516) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3966) [M::mem_pestat] mean and std.dev: (604.30, 617.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5191) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.09, 82.80) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 30, 109) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 297) [M::mem_pestat] mean and std.dev: (36.83, 44.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 391) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1145.684 CPU sec, 67.932 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 661713, 46, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (203, 282, 626) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1472) [M::mem_pestat] mean and std.dev: (428.38, 332.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1895) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (126, 666) [M::mem_pestat] mean and std.dev: (397.17, 82.55) [M::mem_pestat] low and high boundaries for proper pairs: (18, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 52, 753) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2215) [M::mem_pestat] mean and std.dev: (212.87, 484.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2946) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1148.199 CPU sec, 50.795 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 661441, 43, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 401, 1002) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2784) [M::mem_pestat] mean and std.dev: (568.75, 521.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3675) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (126, 666) [M::mem_pestat] mean and std.dev: (397.25, 82.57) [M::mem_pestat] low and high boundaries for proper pairs: (18, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 45, 557) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1619) [M::mem_pestat] mean and std.dev: (153.14, 293.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2150) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1157.882 CPU sec, 64.550 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 661396, 32, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (311, 392, 465) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (3, 773) [M::mem_pestat] mean and std.dev: (335.70, 148.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 930) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (396.97, 82.70) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 51, 1621) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4805) [M::mem_pestat] mean and std.dev: (736.35, 1383.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6397) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1144.877 CPU sec, 50.257 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 662232, 50, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.04, 82.63) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 37, 155) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 429) [M::mem_pestat] mean and std.dev: (36.87, 37.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 566) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1152.957 CPU sec, 67.450 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 662265, 48, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (121, 671) [M::mem_pestat] mean and std.dev: (397.27, 82.92) [M::mem_pestat] low and high boundaries for proper pairs: (11, 781) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 34, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1246) [M::mem_pestat] mean and std.dev: (97.25, 170.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1656) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1146.054 CPU sec, 50.768 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 661915, 51, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (230, 260, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (248.11, 129.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1070) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.41, 82.83) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 42, 93) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 243) [M::mem_pestat] mean and std.dev: (35.73, 28.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 318) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1151.295 CPU sec, 66.691 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 661953, 47, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (182, 289, 651) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1589) [M::mem_pestat] mean and std.dev: (339.11, 240.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2058) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.08, 82.82) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 33, 93) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 249) [M::mem_pestat] mean and std.dev: (38.58, 41.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 327) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1137.160 CPU sec, 50.197 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 662482, 54, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (264, 402, 1012) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2508) [M::mem_pestat] mean and std.dev: (517.56, 424.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3256) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.15, 82.78) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 32, 85) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 221) [M::mem_pestat] mean and std.dev: (33.30, 33.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 289) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1156.689 CPU sec, 67.239 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 662435, 47, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (315, 366, 2129) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5757) [M::mem_pestat] mean and std.dev: (1320.73, 1649.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7921) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.06, 82.72) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 32, 95) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 259) [M::mem_pestat] mean and std.dev: (39.77, 42.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 341) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1141.441 CPU sec, 50.445 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 662164, 50, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.18, 82.79) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 34, 56) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 136) [M::mem_pestat] mean and std.dev: (31.22, 22.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 176) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1160.076 CPU sec, 66.763 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 662519, 63, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (421, 517, 1106) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2476) [M::mem_pestat] mean and std.dev: (674.75, 470.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3161) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.07, 82.71) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 33, 865) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2557) [M::mem_pestat] mean and std.dev: (268.23, 561.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3403) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1134.195 CPU sec, 50.059 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 662357, 68, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (322, 418, 669) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1363) [M::mem_pestat] mean and std.dev: (517.00, 331.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1842) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.01, 82.72) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 45, 498) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1458) [M::mem_pestat] mean and std.dev: (89.41, 176.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1938) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1152.287 CPU sec, 66.607 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 661628, 44, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (409, 610, 2132) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5578) [M::mem_pestat] mean and std.dev: (1093.46, 1085.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7301) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (126, 666) [M::mem_pestat] mean and std.dev: (397.24, 82.49) [M::mem_pestat] low and high boundaries for proper pairs: (18, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 34, 67) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 165) [M::mem_pestat] mean and std.dev: (35.47, 26.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 214) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1140.672 CPU sec, 50.475 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 661512, 45, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (121, 671) [M::mem_pestat] mean and std.dev: (397.27, 82.89) [M::mem_pestat] low and high boundaries for proper pairs: (11, 781) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 39, 2659) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7943) [M::mem_pestat] mean and std.dev: (837.62, 1430.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10585) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1146.725 CPU sec, 66.313 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 661983, 56, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.11, 82.76) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 42, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1269) [M::mem_pestat] mean and std.dev: (87.98, 151.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1686) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1140.974 CPU sec, 50.394 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 662029, 43, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (126, 666) [M::mem_pestat] mean and std.dev: (397.21, 82.68) [M::mem_pestat] low and high boundaries for proper pairs: (18, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 43, 142) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400) [M::mem_pestat] mean and std.dev: (46.86, 59.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 529) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1147.575 CPU sec, 66.046 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 662154, 41, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (285, 331, 493) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909) [M::mem_pestat] mean and std.dev: (345.78, 114.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1117) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (126, 666) [M::mem_pestat] mean and std.dev: (397.23, 82.58) [M::mem_pestat] low and high boundaries for proper pairs: (18, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 34, 892) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2644) [M::mem_pestat] mean and std.dev: (179.26, 381.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3520) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1136.592 CPU sec, 50.226 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 661508, 59, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (195, 384, 474) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1032) [M::mem_pestat] mean and std.dev: (290.88, 143.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1311) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.17, 82.72) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 37, 403) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1175) [M::mem_pestat] mean and std.dev: (124.74, 254.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1561) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1147.659 CPU sec, 67.483 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 661695, 49, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (126, 666) [M::mem_pestat] mean and std.dev: (397.09, 82.57) [M::mem_pestat] low and high boundaries for proper pairs: (18, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 32, 68) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 170) [M::mem_pestat] mean and std.dev: (29.80, 22.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 221) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1138.681 CPU sec, 65.124 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 661791, 47, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.19, 82.70) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 36, 130) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 364) [M::mem_pestat] mean and std.dev: (42.50, 57.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 481) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1137.150 CPU sec, 50.245 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 661935, 48, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (209, 332, 1212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3218) [M::mem_pestat] mean and std.dev: (618.73, 575.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4221) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.20, 82.83) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 54, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 890) [M::mem_pestat] mean and std.dev: (44.27, 51.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1181) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (573, 1044, 1365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2949) [M::mem_pestat] mean and std.dev: (759.75, 382.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3741) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1145.819 CPU sec, 65.823 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 661436, 52, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (126, 666) [M::mem_pestat] mean and std.dev: (397.12, 82.53) [M::mem_pestat] low and high boundaries for proper pairs: (18, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 48, 564) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1654) [M::mem_pestat] mean and std.dev: (177.74, 322.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2199) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1143.491 CPU sec, 50.528 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 661542, 64, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.23, 82.77) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 36, 479) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1407) [M::mem_pestat] mean and std.dev: (92.60, 186.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1871) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1143.544 CPU sec, 65.408 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 662289, 53, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.13, 82.71) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 35, 331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 957) [M::mem_pestat] mean and std.dev: (60.02, 84.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1270) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1142.358 CPU sec, 50.522 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 661584, 58, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.32, 82.78) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 47, 730) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2146) [M::mem_pestat] mean and std.dev: (219.56, 439.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2854) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1141.903 CPU sec, 65.652 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 662626, 55, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.12, 82.88) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 38, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 593) [M::mem_pestat] mean and std.dev: (37.40, 43.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 786) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1135.813 CPU sec, 50.071 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 661584, 44, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (228, 286, 927) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2325) [M::mem_pestat] mean and std.dev: (513.00, 554.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3024) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (121, 671) [M::mem_pestat] mean and std.dev: (397.24, 83.09) [M::mem_pestat] low and high boundaries for proper pairs: (11, 781) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 33, 105) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 275) [M::mem_pestat] mean and std.dev: (38.11, 34.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 360) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1143.390 CPU sec, 65.008 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 662319, 59, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (412, 480, 814) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1618) [M::mem_pestat] mean and std.dev: (498.25, 165.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2020) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.12, 82.76) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 36, 640) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1888) [M::mem_pestat] mean and std.dev: (65.22, 132.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2512) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1125.482 CPU sec, 49.777 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 661927, 55, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.20, 82.75) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 44, 348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1008) [M::mem_pestat] mean and std.dev: (108.94, 197.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1338) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1145.613 CPU sec, 65.526 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 662540, 59, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 386, 960) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2664) [M::mem_pestat] mean and std.dev: (636.00, 637.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3516) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.17, 82.76) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 43, 766) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2270) [M::mem_pestat] mean and std.dev: (165.98, 419.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3022) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1137.276 CPU sec, 50.329 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 662451, 60, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (121, 671) [M::mem_pestat] mean and std.dev: (397.29, 83.04) [M::mem_pestat] low and high boundaries for proper pairs: (11, 781) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (11, 30, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 623) [M::mem_pestat] mean and std.dev: (54.35, 85.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 827) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1142.855 CPU sec, 65.454 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 662467, 50, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.11, 82.87) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (10, 36, 90) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 250) [M::mem_pestat] mean and std.dev: (38.05, 44.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 330) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1131.302 CPU sec, 49.896 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 662053, 54, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.20, 82.88) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 34, 414) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1208) [M::mem_pestat] mean and std.dev: (88.23, 184.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1605) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1141.202 CPU sec, 66.567 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 661757, 63, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (126, 666) [M::mem_pestat] mean and std.dev: (397.10, 82.56) [M::mem_pestat] low and high boundaries for proper pairs: (18, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 40, 629) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1841) [M::mem_pestat] mean and std.dev: (97.20, 227.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2447) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1132.292 CPU sec, 50.092 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 661258, 50, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.31, 82.83) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 47, 944) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2766) [M::mem_pestat] mean and std.dev: (280.79, 624.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3677) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1140.931 CPU sec, 65.792 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 661716, 53, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (325, 543, 981) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2293) [M::mem_pestat] mean and std.dev: (607.20, 388.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2949) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.15, 82.81) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 28, 103) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (34.16, 37.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 364) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1132.941 CPU sec, 49.988 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 662266, 51, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (415, 537, 742) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1396) [M::mem_pestat] mean and std.dev: (505.75, 129.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1723) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.07, 82.76) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 31, 66) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 168) [M::mem_pestat] mean and std.dev: (29.37, 23.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 219) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1135.187 CPU sec, 65.823 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 662071, 35, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.18, 82.78) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 40, 859) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2527) [M::mem_pestat] mean and std.dev: (245.28, 582.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3361) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1128.753 CPU sec, 49.823 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 661344, 46, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (136, 188, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682) [M::mem_pestat] mean and std.dev: (185.56, 90.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 864) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.45, 82.84) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 40, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669) [M::mem_pestat] mean and std.dev: (62.11, 98.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 886) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1144.011 CPU sec, 66.523 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 662038, 55, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.22, 82.73) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 33, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 777) [M::mem_pestat] mean and std.dev: (58.20, 113.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1031) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1138.489 CPU sec, 50.380 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 661785, 50, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.10, 82.78) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 51, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1058) [M::mem_pestat] mean and std.dev: (80.10, 167.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1405) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1142.030 CPU sec, 65.785 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 661805, 52, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.20, 82.77) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 37, 79) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 207) [M::mem_pestat] mean and std.dev: (30.37, 26.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 271) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1131.587 CPU sec, 50.037 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 662305, 45, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (237, 292, 786) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1884) [M::mem_pestat] mean and std.dev: (430.70, 422.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2433) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.08, 82.79) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 35, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 555) [M::mem_pestat] mean and std.dev: (51.14, 60.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 734) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1137.565 CPU sec, 66.214 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 662203, 45, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (211, 265, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904) [M::mem_pestat] mean and std.dev: (242.75, 114.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1135) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.14, 82.78) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 35, 820) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2424) [M::mem_pestat] mean and std.dev: (242.55, 510.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3226) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (209, 1389, 3513) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10121) [M::mem_pestat] mean and std.dev: (2030.00, 2430.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13425) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1127.595 CPU sec, 49.755 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 661518, 43, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (213, 295, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 576) [M::mem_pestat] mean and std.dev: (261.69, 86.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 697) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.02, 82.75) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 35, 159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 437) [M::mem_pestat] mean and std.dev: (45.61, 52.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 576) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1138.143 CPU sec, 65.502 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 661152, 41, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.15, 82.63) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (11, 29, 142) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 404) [M::mem_pestat] mean and std.dev: (47.71, 72.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 535) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1136.315 CPU sec, 50.282 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 662172, 52, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 379, 927) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2647) [M::mem_pestat] mean and std.dev: (474.00, 449.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3507) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.22, 82.82) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 84, 4178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12472) [M::mem_pestat] mean and std.dev: (2283.25, 2922.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16619) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1139.288 CPU sec, 67.112 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 661627, 43, 10) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.04, 82.72) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 48, 452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1310) [M::mem_pestat] mean and std.dev: (92.31, 172.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1739) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (394, 1177, 2885) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7867) [M::mem_pestat] mean and std.dev: (1734.60, 1761.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10358) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1145.786 CPU sec, 66.310 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 661983, 53, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (396.99, 82.72) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 38, 648) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1908) [M::mem_pestat] mean and std.dev: (101.43, 283.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2538) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1137.171 CPU sec, 50.164 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 661878, 58, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (115, 310, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 796) [M::mem_pestat] mean and std.dev: (264.22, 136.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1023) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (121, 671) [M::mem_pestat] mean and std.dev: (397.31, 83.03) [M::mem_pestat] low and high boundaries for proper pairs: (11, 781) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 54, 1524) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4516) [M::mem_pestat] mean and std.dev: (947.70, 1418.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6623) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1141.934 CPU sec, 66.133 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 662168, 47, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.16, 82.79) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 55, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 696) [M::mem_pestat] mean and std.dev: (58.27, 65.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 920) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1132.439 CPU sec, 50.202 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 661422, 53, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (222, 486, 932) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2352) [M::mem_pestat] mean and std.dev: (513.33, 396.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3062) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.12, 82.78) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 43, 132) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 356) [M::mem_pestat] mean and std.dev: (47.35, 46.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 468) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1151.434 CPU sec, 66.132 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 662364, 54, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.11, 82.84) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 57, 740) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2178) [M::mem_pestat] mean and std.dev: (287.88, 495.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2897) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1129.644 CPU sec, 49.872 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 662565, 43, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (121, 671) [M::mem_pestat] mean and std.dev: (397.29, 82.83) [M::mem_pestat] low and high boundaries for proper pairs: (11, 781) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 37, 149) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 419) [M::mem_pestat] mean and std.dev: (43.61, 50.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 554) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1131.981 CPU sec, 64.712 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 662222, 61, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (324, 361, 1137) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2763) [M::mem_pestat] mean and std.dev: (519.60, 425.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3576) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.08, 82.83) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 38, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686) [M::mem_pestat] mean and std.dev: (52.58, 81.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 910) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1126.379 CPU sec, 49.845 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 661549, 62, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.06, 82.64) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (10, 33, 405) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1195) [M::mem_pestat] mean and std.dev: (116.71, 265.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1590) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1138.204 CPU sec, 66.716 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 662266, 55, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.09, 82.83) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 37, 155) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (47.77, 53.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 554) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1124.501 CPU sec, 49.719 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 661965, 57, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.13, 82.83) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 45, 969) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2877) [M::mem_pestat] mean and std.dev: (290.78, 574.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3831) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1142.951 CPU sec, 65.802 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 661991, 45, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (302, 393, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (34, 704) [M::mem_pestat] mean and std.dev: (348.44, 73.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 838) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.07, 82.69) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 40, 78) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 198) [M::mem_pestat] mean and std.dev: (34.89, 31.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 258) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1137.236 CPU sec, 50.267 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 662018, 49, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.02, 82.65) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 32, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (60.88, 105.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 673) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1125.931 CPU sec, 65.140 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 661742, 57, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 306, 833) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2331) [M::mem_pestat] mean and std.dev: (486.93, 451.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3080) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.03, 82.82) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 32, 94) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 252) [M::mem_pestat] mean and std.dev: (34.57, 37.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 331) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1129.350 CPU sec, 50.041 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 661548, 57, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.03, 82.80) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 43, 147) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (55.29, 62.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 528) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1132.555 CPU sec, 65.910 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 662022, 61, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (270, 320, 416) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 708) [M::mem_pestat] mean and std.dev: (304.73, 94.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 854) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.06, 82.56) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 48, 1948) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5812) [M::mem_pestat] mean and std.dev: (821.55, 1440.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7744) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1121.759 CPU sec, 49.666 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 662440, 55, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.06, 82.71) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 42, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 974) [M::mem_pestat] mean and std.dev: (67.18, 125.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1290) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1122.651 CPU sec, 63.616 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 661666, 57, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (289, 536, 863) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2011) [M::mem_pestat] mean and std.dev: (550.92, 321.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2585) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (396.97, 82.73) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 41, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 777) [M::mem_pestat] mean and std.dev: (68.54, 125.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1029) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1122.708 CPU sec, 49.583 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 661333, 42, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (298, 750, 3729) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10591) [M::mem_pestat] mean and std.dev: (1731.08, 2132.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14022) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.38, 82.73) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 39, 89) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 239) [M::mem_pestat] mean and std.dev: (33.73, 27.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 314) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1123.997 CPU sec, 62.734 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 662035, 50, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (195, 333, 2273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6429) [M::mem_pestat] mean and std.dev: (865.27, 929.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8507) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.05, 82.78) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 56, 1293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3837) [M::mem_pestat] mean and std.dev: (409.45, 720.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5109) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1122.878 CPU sec, 49.670 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 662251, 62, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (251, 391, 795) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1883) [M::mem_pestat] mean and std.dev: (564.23, 435.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2427) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.16, 82.66) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 33, 117) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 327) [M::mem_pestat] mean and std.dev: (36.22, 48.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 432) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1127.935 CPU sec, 63.865 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 661900, 43, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 293, 1278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3722) [M::mem_pestat] mean and std.dev: (769.60, 1055.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4993) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (121, 671) [M::mem_pestat] mean and std.dev: (397.20, 82.90) [M::mem_pestat] low and high boundaries for proper pairs: (11, 781) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 43, 1089) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3223) [M::mem_pestat] mean and std.dev: (421.10, 776.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4290) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1127.773 CPU sec, 49.769 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 661790, 54, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.13, 82.70) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 33, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 543) [M::mem_pestat] mean and std.dev: (53.09, 82.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 720) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1124.844 CPU sec, 64.802 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 662357, 43, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (121, 671) [M::mem_pestat] mean and std.dev: (397.24, 82.87) [M::mem_pestat] low and high boundaries for proper pairs: (11, 781) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 39, 52) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 122) [M::mem_pestat] mean and std.dev: (31.43, 23.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 157) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1118.052 CPU sec, 49.444 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 661683, 68, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.05, 82.81) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 42, 87) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 221) [M::mem_pestat] mean and std.dev: (39.21, 31.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 288) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1125.592 CPU sec, 64.564 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 661504, 43, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.00, 82.81) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 45, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640) [M::mem_pestat] mean and std.dev: (64.62, 68.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 846) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1124.800 CPU sec, 49.735 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 662203, 63, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (235, 324, 893) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2209) [M::mem_pestat] mean and std.dev: (433.00, 431.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2867) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.00, 82.58) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 42, 1018) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3022) [M::mem_pestat] mean and std.dev: (280.19, 603.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4024) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1121.642 CPU sec, 64.000 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 661914, 53, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.21, 82.81) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 38, 1071) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3175) [M::mem_pestat] mean and std.dev: (339.88, 712.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4227) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1123.598 CPU sec, 49.671 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 662127, 56, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.13, 82.67) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 34, 90) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 238) [M::mem_pestat] mean and std.dev: (32.82, 29.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 312) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1126.149 CPU sec, 62.523 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 661195, 54, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (174, 229, 386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 810) [M::mem_pestat] mean and std.dev: (282.00, 186.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1028) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.09, 82.65) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 42, 123) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 337) [M::mem_pestat] mean and std.dev: (40.77, 36.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 444) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1140.031 CPU sec, 66.079 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 661720, 38, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (352, 411, 1325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3271) [M::mem_pestat] mean and std.dev: (739.00, 674.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4244) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.16, 82.76) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 37, 84) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 218) [M::mem_pestat] mean and std.dev: (36.10, 32.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 285) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1133.373 CPU sec, 50.076 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 662255, 51, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.10, 82.76) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (11, 38, 100) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 278) [M::mem_pestat] mean and std.dev: (37.32, 40.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 367) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1135.116 CPU sec, 66.231 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 661598, 47, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (177, 354, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798) [M::mem_pestat] mean and std.dev: (251.50, 124.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1005) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.19, 82.69) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 108, 1981) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5897) [M::mem_pestat] mean and std.dev: (891.44, 1356.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7855) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1127.979 CPU sec, 49.750 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 662238, 62, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (128, 339, 1195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3329) [M::mem_pestat] mean and std.dev: (745.80, 827.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4396) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.15, 82.73) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 45, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716) [M::mem_pestat] mean and std.dev: (65.00, 88.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 947) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1126.894 CPU sec, 63.214 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 661962, 67, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.11, 82.77) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 36, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 702) [M::mem_pestat] mean and std.dev: (65.37, 123.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 930) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1117.948 CPU sec, 49.392 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 661896, 59, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (180, 309, 392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 816) [M::mem_pestat] mean and std.dev: (236.55, 109.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1028) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.22, 82.69) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 46, 922) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2716) [M::mem_pestat] mean and std.dev: (195.24, 411.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3613) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1133.408 CPU sec, 65.764 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 661649, 52, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.06, 82.80) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 36, 120) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 334) [M::mem_pestat] mean and std.dev: (28.68, 24.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 441) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1122.787 CPU sec, 49.701 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 661770, 59, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.16, 82.69) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 35, 142) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 390) [M::mem_pestat] mean and std.dev: (40.45, 46.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 514) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1129.165 CPU sec, 65.067 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 661574, 55, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (250, 557, 1503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4009) [M::mem_pestat] mean and std.dev: (754.64, 828.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5262) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.07, 82.68) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 56, 1100) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3252) [M::mem_pestat] mean and std.dev: (362.08, 737.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4328) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1126.206 CPU sec, 49.727 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 661765, 63, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (273, 338, 466) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 852) [M::mem_pestat] mean and std.dev: (318.33, 72.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1045) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.02, 82.82) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 32, 107) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 291) [M::mem_pestat] mean and std.dev: (41.83, 51.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 383) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1135.374 CPU sec, 64.985 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 662106, 63, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (253, 272, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (67, 532) [M::mem_pestat] mean and std.dev: (272.56, 76.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 625) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.07, 82.78) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 43, 1030) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3038) [M::mem_pestat] mean and std.dev: (417.82, 777.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4042) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1126.393 CPU sec, 49.743 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 662473, 44, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.18, 82.78) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 48, 1463) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4353) [M::mem_pestat] mean and std.dev: (364.82, 721.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5798) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1125.428 CPU sec, 63.482 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 662385, 51, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.23, 82.72) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 39, 1229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3657) [M::mem_pestat] mean and std.dev: (278.42, 664.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4871) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1120.201 CPU sec, 49.426 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 662451, 61, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.01, 82.75) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 25, 114) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 318) [M::mem_pestat] mean and std.dev: (27.46, 28.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 420) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1130.325 CPU sec, 64.839 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 662278, 42, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.21, 82.73) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 37, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479) [M::mem_pestat] mean and std.dev: (53.73, 82.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 634) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1123.353 CPU sec, 49.729 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 662096, 50, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (371, 410, 687) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1319) [M::mem_pestat] mean and std.dev: (413.00, 211.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1635) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.24, 82.77) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 46, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 811) [M::mem_pestat] mean and std.dev: (71.46, 124.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1076) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1128.866 CPU sec, 64.911 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 661967, 47, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.02, 82.82) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (11, 37, 95) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 263) [M::mem_pestat] mean and std.dev: (33.00, 34.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 347) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1127.017 CPU sec, 49.858 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 661995, 58, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (213, 396, 1171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3087) [M::mem_pestat] mean and std.dev: (715.10, 738.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4045) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.13, 82.80) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 36, 68) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 166) [M::mem_pestat] mean and std.dev: (42.24, 34.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 215) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1133.948 CPU sec, 65.624 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 662734, 50, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (773, 1010, 1463) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2843) [M::mem_pestat] mean and std.dev: (1112.64, 801.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4318) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (121, 671) [M::mem_pestat] mean and std.dev: (397.23, 83.03) [M::mem_pestat] low and high boundaries for proper pairs: (11, 781) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 44, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (50.77, 55.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 609) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1126.780 CPU sec, 49.845 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 662685, 44, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (289, 352, 379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (109, 559) [M::mem_pestat] mean and std.dev: (311.82, 48.77) [M::mem_pestat] low and high boundaries for proper pairs: (19, 649) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (126, 666) [M::mem_pestat] mean and std.dev: (397.30, 82.58) [M::mem_pestat] low and high boundaries for proper pairs: (18, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 30, 106) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 286) [M::mem_pestat] mean and std.dev: (36.58, 43.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 376) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1135.705 CPU sec, 65.947 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 661549, 46, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (289, 360, 1277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3253) [M::mem_pestat] mean and std.dev: (564.45, 700.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4241) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (126, 666) [M::mem_pestat] mean and std.dev: (397.13, 82.54) [M::mem_pestat] low and high boundaries for proper pairs: (18, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 35, 915) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2711) [M::mem_pestat] mean and std.dev: (99.56, 236.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3609) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1136.600 CPU sec, 50.238 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 662075, 50, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (121, 671) [M::mem_pestat] mean and std.dev: (397.19, 82.92) [M::mem_pestat] low and high boundaries for proper pairs: (11, 781) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 43, 387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1127) [M::mem_pestat] mean and std.dev: (112.81, 224.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1497) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1136.168 CPU sec, 65.377 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 661836, 50, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (286, 686, 1179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2965) [M::mem_pestat] mean and std.dev: (749.60, 612.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3858) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (126, 666) [M::mem_pestat] mean and std.dev: (397.24, 82.60) [M::mem_pestat] low and high boundaries for proper pairs: (18, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 33, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 955) [M::mem_pestat] mean and std.dev: (97.57, 194.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1268) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1125.272 CPU sec, 49.882 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 661582, 62, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.20, 82.84) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 47, 122) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 324) [M::mem_pestat] mean and std.dev: (50.86, 53.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 425) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1134.183 CPU sec, 64.508 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 661943, 50, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (400, 586, 1174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2722) [M::mem_pestat] mean and std.dev: (770.00, 573.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3496) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.05, 82.62) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 43, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643) [M::mem_pestat] mean and std.dev: (49.59, 66.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 853) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1123.856 CPU sec, 49.675 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 662203, 56, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (220, 300, 499) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1057) [M::mem_pestat] mean and std.dev: (313.00, 180.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1336) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.14, 82.72) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 47, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615) [M::mem_pestat] mean and std.dev: (74.29, 108.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 815) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1123.073 CPU sec, 64.086 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 661592, 58, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (173, 339, 1516) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4202) [M::mem_pestat] mean and std.dev: (696.50, 1023.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5545) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.03, 82.82) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 29, 134) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 374) [M::mem_pestat] mean and std.dev: (33.67, 37.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 494) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1119.650 CPU sec, 49.547 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 662044, 71, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (142, 297, 1499) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4213) [M::mem_pestat] mean and std.dev: (449.50, 507.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5570) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (126, 666) [M::mem_pestat] mean and std.dev: (397.23, 82.63) [M::mem_pestat] low and high boundaries for proper pairs: (18, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 39, 752) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2222) [M::mem_pestat] mean and std.dev: (155.19, 395.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2957) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1130.505 CPU sec, 63.643 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 661984, 59, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (396.92, 82.66) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 35, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (44.28, 59.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 661) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1142.075 CPU sec, 68.361 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 662473, 44, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (308, 601, 1688) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4448) [M::mem_pestat] mean and std.dev: (904.12, 817.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5828) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (121, 671) [M::mem_pestat] mean and std.dev: (397.31, 82.94) [M::mem_pestat] low and high boundaries for proper pairs: (11, 781) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 28, 90) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 230) [M::mem_pestat] mean and std.dev: (42.03, 45.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 300) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1128.529 CPU sec, 49.882 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 661640, 45, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.12, 82.70) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 45, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916) [M::mem_pestat] mean and std.dev: (59.22, 91.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1214) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1137.855 CPU sec, 66.165 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 661748, 50, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (260, 380, 2525) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7055) [M::mem_pestat] mean and std.dev: (1408.83, 2022.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9500) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.03, 82.70) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 33, 126) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 354) [M::mem_pestat] mean and std.dev: (50.53, 67.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 468) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1127.797 CPU sec, 49.817 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 662290, 64, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.08, 82.71) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 38, 131) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 349) [M::mem_pestat] mean and std.dev: (43.28, 40.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 458) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1131.380 CPU sec, 65.884 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 662099, 49, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.11, 82.69) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 26, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (33.92, 39.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 662) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1122.170 CPU sec, 49.497 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 662064, 37, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (172, 343, 1872) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5272) [M::mem_pestat] mean and std.dev: (643.92, 831.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6972) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.08, 82.82) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (8, 24, 59) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 161) [M::mem_pestat] mean and std.dev: (23.74, 22.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 212) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1128.715 CPU sec, 65.351 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 662124, 59, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (232, 287, 615) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1381) [M::mem_pestat] mean and std.dev: (343.10, 206.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1764) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (396.96, 82.77) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 33, 146) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 396) [M::mem_pestat] mean and std.dev: (41.20, 38.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 521) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1122.354 CPU sec, 49.472 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 661974, 56, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.06, 82.74) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 38, 1029) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3049) [M::mem_pestat] mean and std.dev: (326.37, 706.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4059) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1130.854 CPU sec, 65.289 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 662369, 57, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (356, 416, 1574) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4010) [M::mem_pestat] mean and std.dev: (713.40, 545.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5228) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (126, 666) [M::mem_pestat] mean and std.dev: (397.28, 82.62) [M::mem_pestat] low and high boundaries for proper pairs: (18, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 45, 1183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3497) [M::mem_pestat] mean and std.dev: (451.36, 852.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4654) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1127.564 CPU sec, 49.764 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 662103, 56, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (126, 666) [M::mem_pestat] mean and std.dev: (397.23, 82.61) [M::mem_pestat] low and high boundaries for proper pairs: (18, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 45, 1735) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5157) [M::mem_pestat] mean and std.dev: (685.96, 1215.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6868) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1141.011 CPU sec, 66.387 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 661960, 57, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (272, 763, 1299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3353) [M::mem_pestat] mean and std.dev: (648.50, 444.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4380) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.13, 82.76) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 26, 128) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 360) [M::mem_pestat] mean and std.dev: (34.18, 52.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 476) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1134.159 CPU sec, 50.224 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 661596, 51, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (309, 422, 1211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3015) [M::mem_pestat] mean and std.dev: (754.46, 741.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3917) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.15, 82.80) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 41, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 602) [M::mem_pestat] mean and std.dev: (54.60, 75.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 797) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1139.670 CPU sec, 67.509 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 661916, 49, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (312, 405, 463) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (10, 765) [M::mem_pestat] mean and std.dev: (353.22, 100.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 916) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (121, 671) [M::mem_pestat] mean and std.dev: (397.25, 82.90) [M::mem_pestat] low and high boundaries for proper pairs: (11, 781) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 40, 101) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 267) [M::mem_pestat] mean and std.dev: (40.20, 40.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 350) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1129.945 CPU sec, 50.005 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 661184, 53, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (236, 302, 949) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2375) [M::mem_pestat] mean and std.dev: (410.00, 328.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3088) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.05, 82.73) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 40, 1755) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5241) [M::mem_pestat] mean and std.dev: (764.67, 1383.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6984) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1132.003 CPU sec, 65.237 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 662247, 70, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (137, 225, 976) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2654) [M::mem_pestat] mean and std.dev: (348.90, 387.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3493) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (126, 666) [M::mem_pestat] mean and std.dev: (397.17, 82.63) [M::mem_pestat] low and high boundaries for proper pairs: (18, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 28, 125) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 345) [M::mem_pestat] mean and std.dev: (31.02, 36.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 455) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1122.115 CPU sec, 49.678 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 662088, 57, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (197, 311, 1075) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2831) [M::mem_pestat] mean and std.dev: (530.08, 571.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3709) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.20, 82.93) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (10, 44, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 541) [M::mem_pestat] mean and std.dev: (53.18, 69.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 718) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1135.075 CPU sec, 66.292 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 661693, 64, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (126, 666) [M::mem_pestat] mean and std.dev: (397.27, 82.59) [M::mem_pestat] low and high boundaries for proper pairs: (18, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 36, 152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (40.45, 48.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 548) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1130.863 CPU sec, 50.040 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 662061, 58, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (126, 666) [M::mem_pestat] mean and std.dev: (397.21, 82.62) [M::mem_pestat] low and high boundaries for proper pairs: (18, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 35, 99) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 267) [M::mem_pestat] mean and std.dev: (39.88, 38.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 351) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1126.813 CPU sec, 64.445 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 662362, 54, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (198, 350, 849) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2151) [M::mem_pestat] mean and std.dev: (428.67, 328.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2802) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.03, 82.77) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (9, 24, 48) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 126) [M::mem_pestat] mean and std.dev: (22.30, 17.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 165) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1126.604 CPU sec, 49.789 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 662557, 54, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (174, 235, 838) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2166) [M::mem_pestat] mean and std.dev: (382.60, 354.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2830) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (121, 671) [M::mem_pestat] mean and std.dev: (397.33, 83.04) [M::mem_pestat] low and high boundaries for proper pairs: (11, 781) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 46, 1115) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3307) [M::mem_pestat] mean and std.dev: (326.59, 726.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4403) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1141.624 CPU sec, 66.332 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 662165, 53, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.27, 82.67) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 35, 159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 451) [M::mem_pestat] mean and std.dev: (50.53, 60.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1129.303 CPU sec, 50.037 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 661993, 51, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (354, 573, 2166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5790) [M::mem_pestat] mean and std.dev: (1173.20, 1420.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7602) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.16, 82.72) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 35, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466) [M::mem_pestat] mean and std.dev: (54.33, 69.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1131.385 CPU sec, 64.953 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 662822, 53, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (276, 354, 500) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948) [M::mem_pestat] mean and std.dev: (334.27, 112.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1172) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.05, 82.89) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 30, 101) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (32.67, 30.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 365) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1122.721 CPU sec, 49.679 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 661694, 53, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (160, 664, 1612) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4516) [M::mem_pestat] mean and std.dev: (883.40, 807.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5968) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.11, 82.75) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 43, 910) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2700) [M::mem_pestat] mean and std.dev: (392.62, 705.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3595) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1134.526 CPU sec, 66.229 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 661945, 49, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (396.94, 82.74) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 35, 1542) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4602) [M::mem_pestat] mean and std.dev: (522.59, 1141.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6132) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1118.182 CPU sec, 49.416 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 661807, 59, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.23, 82.83) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 48, 1581) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4689) [M::mem_pestat] mean and std.dev: (865.33, 1428.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6580) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1128.942 CPU sec, 64.829 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 661548, 55, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (126, 666) [M::mem_pestat] mean and std.dev: (397.18, 82.61) [M::mem_pestat] low and high boundaries for proper pairs: (18, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 35, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697) [M::mem_pestat] mean and std.dev: (59.16, 99.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 922) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1128.217 CPU sec, 49.872 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 661538, 62, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (137, 276, 2467) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7127) [M::mem_pestat] mean and std.dev: (626.33, 933.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9457) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.20, 82.76) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 27, 87) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 233) [M::mem_pestat] mean and std.dev: (38.24, 43.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 306) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1134.791 CPU sec, 62.937 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 661961, 45, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.23, 82.75) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 42, 1357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4023) [M::mem_pestat] mean and std.dev: (477.80, 967.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5356) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1144.708 CPU sec, 66.228 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 662066, 56, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.06, 82.75) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 33, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 791) [M::mem_pestat] mean and std.dev: (54.95, 94.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1049) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1128.644 CPU sec, 49.950 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 660983, 70, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (249, 315, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672) [M::mem_pestat] mean and std.dev: (246.33, 108.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 813) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.09, 82.70) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 53, 1302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3864) [M::mem_pestat] mean and std.dev: (477.48, 841.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5145) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1141.948 CPU sec, 66.299 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 661700, 50, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (195, 279, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 891) [M::mem_pestat] mean and std.dev: (287.92, 169.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1123) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (126, 666) [M::mem_pestat] mean and std.dev: (397.15, 82.60) [M::mem_pestat] low and high boundaries for proper pairs: (18, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 29, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638) [M::mem_pestat] mean and std.dev: (51.26, 96.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 846) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1122.860 CPU sec, 49.746 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 662705, 63, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.20, 82.75) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 52, 120) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 324) [M::mem_pestat] mean and std.dev: (53.98, 53.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 426) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1121.295 CPU sec, 64.285 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 661962, 52, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.20, 82.78) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 50, 845) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2481) [M::mem_pestat] mean and std.dev: (125.81, 259.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3299) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1131.078 CPU sec, 50.206 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 662773, 66, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (163, 233, 2459) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7051) [M::mem_pestat] mean and std.dev: (1296.20, 1915.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9347) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.07, 82.77) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 41, 154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 426) [M::mem_pestat] mean and std.dev: (38.96, 38.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 562) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1136.361 CPU sec, 66.618 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 662817, 45, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (121, 671) [M::mem_pestat] mean and std.dev: (397.26, 82.95) [M::mem_pestat] low and high boundaries for proper pairs: (11, 781) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 34, 142) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 386) [M::mem_pestat] mean and std.dev: (36.91, 31.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 508) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1119.003 CPU sec, 49.646 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 662132, 50, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.22, 82.75) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 26, 642) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1902) [M::mem_pestat] mean and std.dev: (109.00, 282.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2532) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1136.542 CPU sec, 66.429 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 662432, 63, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.23, 82.73) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 40, 117) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 317) [M::mem_pestat] mean and std.dev: (41.38, 40.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 417) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1126.683 CPU sec, 49.851 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 662448, 55, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (289, 829, 1580) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4162) [M::mem_pestat] mean and std.dev: (899.40, 731.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5453) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.10, 82.83) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 31, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770) [M::mem_pestat] mean and std.dev: (49.74, 90.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1021) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1137.435 CPU sec, 66.252 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 662567, 58, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.13, 82.83) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 32, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 528) [M::mem_pestat] mean and std.dev: (64.15, 112.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 698) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1123.582 CPU sec, 49.719 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 662413, 53, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (118, 305, 375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 889) [M::mem_pestat] mean and std.dev: (288.92, 207.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1146) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.12, 82.72) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 31, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 575) [M::mem_pestat] mean and std.dev: (44.40, 57.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1127.336 CPU sec, 65.384 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 661875, 60, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (329, 543, 1690) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4412) [M::mem_pestat] mean and std.dev: (882.46, 780.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5773) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.11, 82.79) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 36, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 811) [M::mem_pestat] mean and std.dev: (51.45, 90.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1077) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1125.001 CPU sec, 49.812 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 662197, 67, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.08, 82.79) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 49, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1057) [M::mem_pestat] mean and std.dev: (102.29, 195.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1402) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1120.454 CPU sec, 64.679 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 661738, 46, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.04, 82.80) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 37, 111) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 297) [M::mem_pestat] mean and std.dev: (34.97, 33.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 390) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1129.532 CPU sec, 49.922 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 661995, 55, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (396.92, 82.73) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 53, 1488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4418) [M::mem_pestat] mean and std.dev: (719.67, 1239.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5883) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1131.285 CPU sec, 64.935 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 662164, 59, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (234, 344, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861) [M::mem_pestat] mean and std.dev: (271.88, 122.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1070) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.01, 82.86) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 28, 75) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 191) [M::mem_pestat] mean and std.dev: (34.57, 35.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 249) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1123.355 CPU sec, 49.738 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 662597, 51, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (204, 330, 666) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1590) [M::mem_pestat] mean and std.dev: (402.22, 338.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2052) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.21, 82.81) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 28, 83) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 223) [M::mem_pestat] mean and std.dev: (29.70, 35.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 293) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1133.627 CPU sec, 65.535 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 662266, 47, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.07, 82.73) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 41, 553) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1615) [M::mem_pestat] mean and std.dev: (115.03, 263.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2146) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1135.513 CPU sec, 66.507 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 661989, 48, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (212, 263, 504) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1088) [M::mem_pestat] mean and std.dev: (280.08, 184.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1380) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.13, 82.71) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 51, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952) [M::mem_pestat] mean and std.dev: (74.08, 122.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1264) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1127.990 CPU sec, 49.975 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 661991, 48, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (396.99, 82.63) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 31, 104) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 286) [M::mem_pestat] mean and std.dev: (26.57, 24.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 377) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1129.944 CPU sec, 64.327 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 662209, 52, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (225, 341, 528) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1134) [M::mem_pestat] mean and std.dev: (331.09, 248.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1437) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.09, 82.64) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 45, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497) [M::mem_pestat] mean and std.dev: (49.68, 49.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 655) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1131.362 CPU sec, 50.019 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 661747, 56, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (300, 482, 780) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1740) [M::mem_pestat] mean and std.dev: (488.00, 364.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2220) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (126, 666) [M::mem_pestat] mean and std.dev: (397.21, 82.60) [M::mem_pestat] low and high boundaries for proper pairs: (18, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 34, 1151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3427) [M::mem_pestat] mean and std.dev: (342.78, 717.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4565) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1131.999 CPU sec, 65.711 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 661716, 48, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (204, 344, 815) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2037) [M::mem_pestat] mean and std.dev: (621.50, 579.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2941) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.16, 82.84) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 58, 495) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1439) [M::mem_pestat] mean and std.dev: (165.25, 298.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1911) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1128.645 CPU sec, 49.893 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 661142, 58, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.31, 82.71) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 28, 124) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 344) [M::mem_pestat] mean and std.dev: (47.14, 64.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 454) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1134.385 CPU sec, 65.133 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 662273, 54, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.20, 82.76) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 30, 59) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 153) [M::mem_pestat] mean and std.dev: (28.82, 28.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 200) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1123.430 CPU sec, 49.678 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 662262, 60, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (339, 391, 710) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1452) [M::mem_pestat] mean and std.dev: (475.80, 255.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1823) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.11, 82.73) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 32, 55) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 119) [M::mem_pestat] mean and std.dev: (34.68, 22.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 151) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1128.562 CPU sec, 65.605 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 662477, 59, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.05, 82.73) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (11, 22, 55) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 143) [M::mem_pestat] mean and std.dev: (27.10, 22.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 187) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1123.650 CPU sec, 49.658 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 661706, 34, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.09, 82.75) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (11, 29, 144) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (29.54, 31.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 543) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1133.096 CPU sec, 65.516 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 662135, 63, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.18, 82.79) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 44, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497) [M::mem_pestat] mean and std.dev: (46.38, 50.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 655) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1124.623 CPU sec, 49.809 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 661572, 55, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (396.99, 82.67) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 42, 1043) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3097) [M::mem_pestat] mean and std.dev: (223.52, 513.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4124) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1131.779 CPU sec, 64.937 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 661855, 43, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.11, 82.75) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 36, 114) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 306) [M::mem_pestat] mean and std.dev: (32.09, 31.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 402) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1125.895 CPU sec, 49.851 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 661466, 54, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (398, 425, 919) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1961) [M::mem_pestat] mean and std.dev: (599.80, 478.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2515) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.37, 82.73) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 51, 561) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1637) [M::mem_pestat] mean and std.dev: (151.39, 316.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2175) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1128.922 CPU sec, 64.795 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 661708, 58, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (234, 382, 529) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1119) [M::mem_pestat] mean and std.dev: (355.31, 254.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1414) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.01, 82.65) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 34, 147) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (30.86, 29.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 540) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1124.280 CPU sec, 49.726 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 661982, 46, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (255, 262, 472) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906) [M::mem_pestat] mean and std.dev: (261.78, 98.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1123) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.15, 82.77) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 27, 73) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 191) [M::mem_pestat] mean and std.dev: (29.95, 31.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 250) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1133.686 CPU sec, 65.601 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 662267, 49, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (297, 318, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 759) [M::mem_pestat] mean and std.dev: (323.11, 69.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 913) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.07, 82.71) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 44, 299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 869) [M::mem_pestat] mean and std.dev: (65.30, 102.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1154) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1124.015 CPU sec, 49.674 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 661359, 53, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (250, 321, 603) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1309) [M::mem_pestat] mean and std.dev: (362.55, 203.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1662) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (121, 671) [M::mem_pestat] mean and std.dev: (397.34, 83.00) [M::mem_pestat] low and high boundaries for proper pairs: (11, 781) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 26, 58) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 144) [M::mem_pestat] mean and std.dev: (29.98, 28.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 187) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1125.684 CPU sec, 64.977 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 662972, 61, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (121, 671) [M::mem_pestat] mean and std.dev: (397.30, 82.99) [M::mem_pestat] low and high boundaries for proper pairs: (11, 781) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 30, 502) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1480) [M::mem_pestat] mean and std.dev: (110.49, 255.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1969) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1121.010 CPU sec, 49.616 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 662068, 50, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.27, 82.70) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 59, 1181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3501) [M::mem_pestat] mean and std.dev: (434.49, 818.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4661) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1136.131 CPU sec, 65.787 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 662934, 49, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (126, 666) [M::mem_pestat] mean and std.dev: (397.23, 82.66) [M::mem_pestat] low and high boundaries for proper pairs: (18, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 38, 1186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3514) [M::mem_pestat] mean and std.dev: (506.66, 907.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4678) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1120.913 CPU sec, 49.637 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 661860, 42, 10) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.14, 82.71) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 42, 913) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2713) [M::mem_pestat] mean and std.dev: (347.68, 744.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3613) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (424, 989, 2119) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5509) [M::mem_pestat] mean and std.dev: (1366.70, 1148.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7204) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1131.399 CPU sec, 65.126 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 661868, 60, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.15, 82.69) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 33, 461) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1355) [M::mem_pestat] mean and std.dev: (103.51, 250.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1802) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1124.281 CPU sec, 49.640 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 661916, 58, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.09, 82.66) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 34, 125) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 349) [M::mem_pestat] mean and std.dev: (45.08, 53.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1128.282 CPU sec, 64.120 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 661676, 48, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.22, 82.89) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 30, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462) [M::mem_pestat] mean and std.dev: (36.53, 44.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 611) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1122.250 CPU sec, 49.653 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 662159, 58, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (269, 347, 413) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701) [M::mem_pestat] mean and std.dev: (319.23, 100.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 845) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.08, 82.74) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 41, 1050) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3120) [M::mem_pestat] mean and std.dev: (288.04, 633.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4155) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1119.448 CPU sec, 62.906 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 662009, 66, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.08, 82.79) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 37, 104) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 268) [M::mem_pestat] mean and std.dev: (41.00, 40.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 350) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1120.540 CPU sec, 49.537 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 661989, 54, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.16, 82.71) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 82, 1936) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5752) [M::mem_pestat] mean and std.dev: (752.39, 1331.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7660) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1119.773 CPU sec, 63.683 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 661890, 49, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.07, 82.73) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 36, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751) [M::mem_pestat] mean and std.dev: (68.05, 97.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 996) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1137.721 CPU sec, 67.138 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 661983, 57, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (126, 666) [M::mem_pestat] mean and std.dev: (397.30, 82.63) [M::mem_pestat] low and high boundaries for proper pairs: (18, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 44, 98) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 250) [M::mem_pestat] mean and std.dev: (35.86, 22.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 326) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1126.709 CPU sec, 49.832 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 661718, 47, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.43, 82.93) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 45, 1529) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4553) [M::mem_pestat] mean and std.dev: (793.11, 1392.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6363) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1129.516 CPU sec, 65.188 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 662188, 36, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (227, 273, 650) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1496) [M::mem_pestat] mean and std.dev: (394.60, 234.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1919) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (126, 666) [M::mem_pestat] mean and std.dev: (397.05, 82.58) [M::mem_pestat] low and high boundaries for proper pairs: (18, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 50, 646) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1908) [M::mem_pestat] mean and std.dev: (216.56, 390.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2539) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1117.829 CPU sec, 49.279 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 661804, 64, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.12, 82.69) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 26, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (41.88, 62.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 639) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1120.022 CPU sec, 63.881 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 662018, 34, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (159, 502, 966) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2580) [M::mem_pestat] mean and std.dev: (535.40, 403.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3387) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (121, 671) [M::mem_pestat] mean and std.dev: (397.22, 82.93) [M::mem_pestat] low and high boundaries for proper pairs: (11, 781) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 96, 1566) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4656) [M::mem_pestat] mean and std.dev: (757.16, 1317.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6201) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1123.228 CPU sec, 49.641 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 662716, 40, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.12, 82.67) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (10, 30, 141) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403) [M::mem_pestat] mean and std.dev: (53.41, 82.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 534) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1121.997 CPU sec, 65.438 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 661600, 55, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (121, 671) [M::mem_pestat] mean and std.dev: (397.25, 82.89) [M::mem_pestat] low and high boundaries for proper pairs: (11, 781) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 24, 123) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 341) [M::mem_pestat] mean and std.dev: (28.14, 24.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 450) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1127.298 CPU sec, 49.824 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 661833, 50, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (372, 465, 1813) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4695) [M::mem_pestat] mean and std.dev: (828.60, 755.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6136) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (126, 666) [M::mem_pestat] mean and std.dev: (397.33, 82.66) [M::mem_pestat] low and high boundaries for proper pairs: (18, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 37, 79) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 197) [M::mem_pestat] mean and std.dev: (30.95, 21.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 256) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1130.386 CPU sec, 65.074 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 661751, 41, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (277, 671, 1417) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3697) [M::mem_pestat] mean and std.dev: (745.08, 691.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4837) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.10, 82.73) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 33, 82) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 222) [M::mem_pestat] mean and std.dev: (38.61, 36.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 292) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1124.322 CPU sec, 49.705 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 662370, 45, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.29, 82.69) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 34, 115) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 309) [M::mem_pestat] mean and std.dev: (33.83, 35.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 406) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1131.165 CPU sec, 64.634 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 661839, 58, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (189, 272, 376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750) [M::mem_pestat] mean and std.dev: (237.90, 106.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 937) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.11, 82.77) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 35, 135) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 373) [M::mem_pestat] mean and std.dev: (41.57, 47.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 492) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1117.313 CPU sec, 49.283 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 661792, 53, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 400, 481) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1093) [M::mem_pestat] mean and std.dev: (405.30, 323.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1701) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.04, 82.64) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 29, 68) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 170) [M::mem_pestat] mean and std.dev: (30.68, 25.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 221) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1132.182 CPU sec, 64.402 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 661767, 51, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.22, 82.75) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 29, 93) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 249) [M::mem_pestat] mean and std.dev: (32.27, 28.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 327) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1119.406 CPU sec, 49.571 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 661810, 56, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.10, 82.77) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 25, 41) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 97) [M::mem_pestat] mean and std.dev: (22.71, 14.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 125) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1124.535 CPU sec, 63.577 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 661942, 47, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (145, 247, 425) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 985) [M::mem_pestat] mean and std.dev: (282.20, 194.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1265) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (126, 666) [M::mem_pestat] mean and std.dev: (397.23, 82.73) [M::mem_pestat] low and high boundaries for proper pairs: (18, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 32, 1026) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3048) [M::mem_pestat] mean and std.dev: (464.45, 867.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4059) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1120.901 CPU sec, 49.622 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 661899, 59, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 496, 876) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2290) [M::mem_pestat] mean and std.dev: (454.64, 348.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2997) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.18, 82.69) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (11, 33, 54) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 140) [M::mem_pestat] mean and std.dev: (24.60, 17.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 183) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1122.042 CPU sec, 63.332 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 661558, 56, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (126, 666) [M::mem_pestat] mean and std.dev: (397.22, 82.82) [M::mem_pestat] low and high boundaries for proper pairs: (18, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 32, 150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424) [M::mem_pestat] mean and std.dev: (44.22, 69.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 561) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1125.008 CPU sec, 49.738 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 661856, 45, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.09, 82.72) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 37, 996) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2952) [M::mem_pestat] mean and std.dev: (251.13, 504.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3930) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1119.994 CPU sec, 63.607 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 661942, 57, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (261, 317, 1037) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2589) [M::mem_pestat] mean and std.dev: (471.92, 403.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3365) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.08, 82.77) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 58, 2596) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7732) [M::mem_pestat] mean and std.dev: (1111.32, 1642.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10300) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1115.426 CPU sec, 49.273 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 662240, 65, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 236, 1062) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2836) [M::mem_pestat] mean and std.dev: (370.50, 321.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3723) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.14, 82.71) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 41, 631) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1867) [M::mem_pestat] mean and std.dev: (159.83, 381.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2485) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1131.301 CPU sec, 64.950 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 662060, 54, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (263, 387, 1129) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2861) [M::mem_pestat] mean and std.dev: (529.00, 506.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3727) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (121, 671) [M::mem_pestat] mean and std.dev: (397.18, 82.93) [M::mem_pestat] low and high boundaries for proper pairs: (11, 781) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 52, 2271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6781) [M::mem_pestat] mean and std.dev: (1229.45, 2049.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9429) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1124.020 CPU sec, 49.766 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 661379, 53, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.05, 82.70) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 37, 1041) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3095) [M::mem_pestat] mean and std.dev: (405.19, 812.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4122) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1130.108 CPU sec, 63.469 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 661861, 58, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.03, 82.80) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 43, 137) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 375) [M::mem_pestat] mean and std.dev: (52.62, 61.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 494) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1129.531 CPU sec, 49.899 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 662017, 52, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.22, 82.83) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 42, 90) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 226) [M::mem_pestat] mean and std.dev: (40.10, 36.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 294) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1121.191 CPU sec, 64.140 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 662365, 66, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (250, 477, 783) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1849) [M::mem_pestat] mean and std.dev: (370.75, 205.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2382) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.14, 82.78) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 42, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 882) [M::mem_pestat] mean and std.dev: (74.38, 128.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1171) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1120.905 CPU sec, 49.503 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 661679, 61, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.11, 82.70) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 28, 68) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 174) [M::mem_pestat] mean and std.dev: (27.46, 20.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 227) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1126.289 CPU sec, 64.960 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 662015, 50, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (126, 666) [M::mem_pestat] mean and std.dev: (397.21, 82.57) [M::mem_pestat] low and high boundaries for proper pairs: (18, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 30, 105) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 291) [M::mem_pestat] mean and std.dev: (31.55, 29.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 384) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1124.770 CPU sec, 49.688 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 662401, 56, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (126, 666) [M::mem_pestat] mean and std.dev: (397.25, 82.64) [M::mem_pestat] low and high boundaries for proper pairs: (18, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 62, 833) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2461) [M::mem_pestat] mean and std.dev: (279.76, 542.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3275) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1125.079 CPU sec, 63.312 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 662049, 42, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.20, 82.81) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 59, 1438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4260) [M::mem_pestat] mean and std.dev: (446.16, 872.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5671) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1141.976 CPU sec, 66.657 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 662567, 57, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (210, 303, 762) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1866) [M::mem_pestat] mean and std.dev: (462.80, 328.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2418) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.12, 82.90) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 50, 522) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1528) [M::mem_pestat] mean and std.dev: (142.00, 282.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2031) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1135.723 CPU sec, 50.158 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 662911, 58, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (201, 442, 939) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2415) [M::mem_pestat] mean and std.dev: (592.15, 571.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3153) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.20, 82.78) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 42, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660) [M::mem_pestat] mean and std.dev: (89.94, 151.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 874) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1141.051 CPU sec, 65.920 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 661781, 48, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.20, 82.62) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 35, 110) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 304) [M::mem_pestat] mean and std.dev: (30.76, 25.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 401) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1144.592 CPU sec, 50.867 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 661520, 55, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.01, 82.69) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 42, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 734) [M::mem_pestat] mean and std.dev: (47.81, 58.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 973) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1157.181 CPU sec, 67.509 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 661651, 64, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (396.99, 82.79) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 32, 48) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 118) [M::mem_pestat] mean and std.dev: (23.12, 13.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 153) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1151.608 CPU sec, 51.123 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 662033, 55, 10) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.18, 82.75) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 44, 124) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 326) [M::mem_pestat] mean and std.dev: (50.78, 46.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 427) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (507, 1149, 1200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2586) [M::mem_pestat] mean and std.dev: (945.33, 680.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3667) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1157.794 CPU sec, 66.935 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 661949, 62, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.14, 82.71) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 41, 430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1260) [M::mem_pestat] mean and std.dev: (113.26, 248.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1675) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1150.142 CPU sec, 51.015 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 662363, 59, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (150, 272, 1681) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4743) [M::mem_pestat] mean and std.dev: (389.33, 481.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6274) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.06, 82.61) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 40, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1272) [M::mem_pestat] mean and std.dev: (118.71, 265.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1689) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1151.946 CPU sec, 65.745 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 661787, 69, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (144, 358, 723) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1881) [M::mem_pestat] mean and std.dev: (304.12, 209.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2460) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.11, 82.77) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 31, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 587) [M::mem_pestat] mean and std.dev: (54.58, 88.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 777) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1157.992 CPU sec, 51.343 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 661719, 54, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (396.96, 82.71) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 35, 69) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 171) [M::mem_pestat] mean and std.dev: (31.98, 24.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 222) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1153.265 CPU sec, 65.690 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 661401, 53, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.14, 82.74) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 44, 1252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3716) [M::mem_pestat] mean and std.dev: (481.55, 917.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4948) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1152.594 CPU sec, 50.970 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 661260, 64, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.26, 82.84) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 62, 780) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2292) [M::mem_pestat] mean and std.dev: (280.98, 528.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3048) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1162.136 CPU sec, 67.302 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 661778, 55, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (121, 671) [M::mem_pestat] mean and std.dev: (397.25, 82.85) [M::mem_pestat] low and high boundaries for proper pairs: (11, 781) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 37, 71) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 173) [M::mem_pestat] mean and std.dev: (37.15, 27.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 224) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1160.586 CPU sec, 51.444 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 661970, 55, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (121, 671) [M::mem_pestat] mean and std.dev: (397.12, 82.92) [M::mem_pestat] low and high boundaries for proper pairs: (11, 781) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 47, 923) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2737) [M::mem_pestat] mean and std.dev: (298.96, 658.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3644) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1153.632 CPU sec, 65.913 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 662585, 39, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (294, 601, 1792) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4788) [M::mem_pestat] mean and std.dev: (872.64, 889.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6286) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.10, 82.85) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 38, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 571) [M::mem_pestat] mean and std.dev: (56.13, 95.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 755) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1142.931 CPU sec, 50.557 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 661966, 55, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.25, 82.79) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 34, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 574) [M::mem_pestat] mean and std.dev: (62.41, 98.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 760) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1159.414 CPU sec, 67.011 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 661873, 50, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.13, 82.72) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 37, 152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (45.30, 54.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 551) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1154.559 CPU sec, 51.016 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 662897, 61, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (126, 666) [M::mem_pestat] mean and std.dev: (397.26, 82.73) [M::mem_pestat] low and high boundaries for proper pairs: (18, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 35, 3284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9820) [M::mem_pestat] mean and std.dev: (1552.62, 2596.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13088) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1151.249 CPU sec, 67.699 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 663009, 40, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.10, 82.79) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 32, 111) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 307) [M::mem_pestat] mean and std.dev: (45.76, 57.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 405) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1150.511 CPU sec, 50.864 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 661577, 42, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 329, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 885) [M::mem_pestat] mean and std.dev: (185.50, 126.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1156) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.33, 82.81) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 32, 59) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 141) [M::mem_pestat] mean and std.dev: (24.97, 14.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 182) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1155.153 CPU sec, 67.411 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 661263, 60, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.17, 82.71) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 36, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 613) [M::mem_pestat] mean and std.dev: (54.45, 94.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 813) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1150.705 CPU sec, 50.759 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 662269, 51, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (399, 588, 1280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3042) [M::mem_pestat] mean and std.dev: (884.70, 783.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4020) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.06, 82.70) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 34, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 780) [M::mem_pestat] mean and std.dev: (42.44, 50.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1035) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1153.689 CPU sec, 66.769 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 662226, 48, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (121, 671) [M::mem_pestat] mean and std.dev: (397.26, 82.77) [M::mem_pestat] low and high boundaries for proper pairs: (11, 781) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 35, 62) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 158) [M::mem_pestat] mean and std.dev: (29.00, 19.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 206) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1143.009 CPU sec, 50.542 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 661794, 51, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.08, 82.68) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 38, 74) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 184) [M::mem_pestat] mean and std.dev: (33.88, 24.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 239) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1150.723 CPU sec, 66.961 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 661887, 64, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (253, 306, 717) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1645) [M::mem_pestat] mean and std.dev: (490.86, 367.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2109) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (126, 666) [M::mem_pestat] mean and std.dev: (397.15, 82.59) [M::mem_pestat] low and high boundaries for proper pairs: (18, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 37, 69) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 171) [M::mem_pestat] mean and std.dev: (31.85, 21.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 222) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1142.956 CPU sec, 50.474 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 662208, 39, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (168, 262, 1243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3393) [M::mem_pestat] mean and std.dev: (827.82, 947.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4618) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.18, 82.74) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 37, 1922) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5724) [M::mem_pestat] mean and std.dev: (739.82, 1324.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7625) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1150.034 CPU sec, 67.915 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 661917, 52, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.13, 82.68) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 44, 803) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2363) [M::mem_pestat] mean and std.dev: (148.67, 373.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3143) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1142.772 CPU sec, 50.509 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 662933, 53, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (362, 661, 1940) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5096) [M::mem_pestat] mean and std.dev: (1164.70, 1200.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6674) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (126, 666) [M::mem_pestat] mean and std.dev: (397.26, 82.64) [M::mem_pestat] low and high boundaries for proper pairs: (18, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 53, 1121) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3321) [M::mem_pestat] mean and std.dev: (490.46, 885.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4421) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1143.399 CPU sec, 61.787 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 662460, 47, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.19, 82.70) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 49, 596) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1734) [M::mem_pestat] mean and std.dev: (287.81, 508.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2320) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1145.337 CPU sec, 66.731 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 661644, 58, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (302, 931, 1273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3215) [M::mem_pestat] mean and std.dev: (898.80, 679.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4186) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.33, 82.83) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 41, 86) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 214) [M::mem_pestat] mean and std.dev: (41.65, 30.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 278) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1144.038 CPU sec, 50.642 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 661816, 51, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (163, 249, 487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1135) [M::mem_pestat] mean and std.dev: (281.58, 192.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1459) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.24, 82.75) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 28, 59) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 145) [M::mem_pestat] mean and std.dev: (28.15, 24.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 188) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1149.919 CPU sec, 68.287 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 661823, 45, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.18, 82.71) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 26, 59) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 145) [M::mem_pestat] mean and std.dev: (29.26, 21.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 188) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1137.013 CPU sec, 50.266 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 661868, 42, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.13, 82.80) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 36, 114) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 316) [M::mem_pestat] mean and std.dev: (32.24, 31.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 417) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1153.135 CPU sec, 67.535 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 661491, 56, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (228, 275, 433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 843) [M::mem_pestat] mean and std.dev: (237.77, 113.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1048) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.12, 82.84) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 35, 773) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2291) [M::mem_pestat] mean and std.dev: (124.56, 302.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3050) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1146.945 CPU sec, 50.768 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 662029, 52, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.14, 82.80) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 43, 409) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1193) [M::mem_pestat] mean and std.dev: (133.00, 282.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1585) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1141.609 CPU sec, 67.047 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 661909, 55, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.03, 82.61) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 39, 1039) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3087) [M::mem_pestat] mean and std.dev: (250.15, 590.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4111) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1140.163 CPU sec, 50.468 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 661800, 54, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (121, 671) [M::mem_pestat] mean and std.dev: (397.23, 82.96) [M::mem_pestat] low and high boundaries for proper pairs: (11, 781) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 33, 76) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 198) [M::mem_pestat] mean and std.dev: (35.13, 30.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 259) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1146.464 CPU sec, 66.459 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 662040, 53, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (293, 357, 4319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12371) [M::mem_pestat] mean and std.dev: (1701.55, 2343.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16397) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (126, 666) [M::mem_pestat] mean and std.dev: (397.19, 82.57) [M::mem_pestat] low and high boundaries for proper pairs: (18, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 39, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1044) [M::mem_pestat] mean and std.dev: (50.38, 78.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1387) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1139.455 CPU sec, 50.412 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 662122, 50, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (273, 415, 764) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1746) [M::mem_pestat] mean and std.dev: (463.56, 296.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2237) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.22, 82.70) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 52, 771) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2273) [M::mem_pestat] mean and std.dev: (227.42, 392.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3024) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1147.683 CPU sec, 65.797 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 661798, 69, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.11, 82.76) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 38, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 887) [M::mem_pestat] mean and std.dev: (85.21, 169.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1176) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1142.716 CPU sec, 50.530 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 661481, 45, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (163, 279, 1219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3331) [M::mem_pestat] mean and std.dev: (707.21, 826.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4387) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.20, 82.81) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 41, 375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1089) [M::mem_pestat] mean and std.dev: (61.00, 113.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1446) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1143.976 CPU sec, 65.897 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 662576, 75, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (126, 666) [M::mem_pestat] mean and std.dev: (397.27, 82.72) [M::mem_pestat] low and high boundaries for proper pairs: (18, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (10, 27, 147) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (32.71, 53.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 558) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1140.416 CPU sec, 50.545 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 661590, 59, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.06, 82.76) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 48, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 696) [M::mem_pestat] mean and std.dev: (58.11, 74.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 922) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1143.652 CPU sec, 66.161 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 662058, 56, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (356, 481, 1416) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3536) [M::mem_pestat] mean and std.dev: (683.90, 450.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4596) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.20, 82.80) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 54, 1649) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4913) [M::mem_pestat] mean and std.dev: (732.11, 1225.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6545) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1139.054 CPU sec, 50.430 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 662135, 57, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.06, 82.78) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 31, 76) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 202) [M::mem_pestat] mean and std.dev: (29.74, 25.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 265) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1140.383 CPU sec, 67.121 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 662643, 63, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (177, 835, 932) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2442) [M::mem_pestat] mean and std.dev: (544.44, 370.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3197) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.17, 82.78) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 54, 543) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1593) [M::mem_pestat] mean and std.dev: (165.52, 316.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2118) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1139.216 CPU sec, 50.310 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 661458, 53, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (121, 671) [M::mem_pestat] mean and std.dev: (397.21, 82.93) [M::mem_pestat] low and high boundaries for proper pairs: (11, 781) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 32, 1599) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4773) [M::mem_pestat] mean and std.dev: (774.35, 1395.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6360) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1150.060 CPU sec, 68.299 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 662277, 50, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (359, 689, 1970) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5192) [M::mem_pestat] mean and std.dev: (944.50, 769.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6803) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.27, 82.76) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 44, 110) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 282) [M::mem_pestat] mean and std.dev: (44.54, 35.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 368) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1142.197 CPU sec, 50.495 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 662069, 58, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (165, 278, 1290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3540) [M::mem_pestat] mean and std.dev: (549.75, 813.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4665) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.05, 82.65) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 28, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 999) [M::mem_pestat] mean and std.dev: (60.11, 120.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1328) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1146.641 CPU sec, 67.705 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 661780, 60, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (121, 671) [M::mem_pestat] mean and std.dev: (397.21, 83.00) [M::mem_pestat] low and high boundaries for proper pairs: (11, 781) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 48, 939) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2775) [M::mem_pestat] mean and std.dev: (336.02, 692.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3693) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1136.040 CPU sec, 50.253 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 662127, 63, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.02, 82.75) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 32, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 850) [M::mem_pestat] mean and std.dev: (76.26, 150.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1128) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1137.750 CPU sec, 64.677 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 662291, 57, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.09, 82.74) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 41, 781) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2311) [M::mem_pestat] mean and std.dev: (268.48, 533.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3076) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1138.148 CPU sec, 50.347 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 661299, 55, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (326, 373, 681) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1391) [M::mem_pestat] mean and std.dev: (443.73, 233.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1746) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.16, 82.71) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 38, 498) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1456) [M::mem_pestat] mean and std.dev: (139.65, 277.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1935) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1146.736 CPU sec, 66.401 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 662042, 68, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (126, 666) [M::mem_pestat] mean and std.dev: (397.21, 82.53) [M::mem_pestat] low and high boundaries for proper pairs: (18, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 33, 129) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 351) [M::mem_pestat] mean and std.dev: (37.93, 42.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1131.858 CPU sec, 49.937 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 661876, 47, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (217, 264, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (27, 502) [M::mem_pestat] mean and std.dev: (217.50, 85.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.16, 82.77) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 30, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 575) [M::mem_pestat] mean and std.dev: (42.54, 51.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 762) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1145.828 CPU sec, 65.461 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 661930, 50, 11) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.16, 82.63) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 45, 159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 437) [M::mem_pestat] mean and std.dev: (45.82, 48.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 576) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (939, 1453, 2326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5100) [M::mem_pestat] mean and std.dev: (1547.64, 1101.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6487) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1136.376 CPU sec, 50.290 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 662195, 59, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.14, 82.78) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 36, 144) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400) [M::mem_pestat] mean and std.dev: (37.41, 36.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 528) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1137.331 CPU sec, 63.883 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 661607, 46, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.28, 82.78) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 47, 128) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 348) [M::mem_pestat] mean and std.dev: (44.72, 41.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 458) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1145.848 CPU sec, 66.680 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 661982, 65, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.17, 82.75) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 31, 131) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 367) [M::mem_pestat] mean and std.dev: (42.67, 61.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 485) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1137.259 CPU sec, 50.292 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 662041, 60, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (121, 671) [M::mem_pestat] mean and std.dev: (397.32, 82.87) [M::mem_pestat] low and high boundaries for proper pairs: (11, 781) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 47, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 873) [M::mem_pestat] mean and std.dev: (81.49, 146.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1157) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1147.363 CPU sec, 66.971 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 661825, 50, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.46, 82.86) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 34, 128) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 360) [M::mem_pestat] mean and std.dev: (39.38, 47.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 476) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1134.694 CPU sec, 50.192 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 661969, 50, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (121, 671) [M::mem_pestat] mean and std.dev: (397.20, 83.00) [M::mem_pestat] low and high boundaries for proper pairs: (11, 781) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 42, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 589) [M::mem_pestat] mean and std.dev: (68.37, 110.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 779) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1149.907 CPU sec, 66.036 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 661935, 55, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.08, 82.95) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 42, 159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 435) [M::mem_pestat] mean and std.dev: (48.33, 46.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1135.261 CPU sec, 50.343 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 661863, 56, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (121, 671) [M::mem_pestat] mean and std.dev: (397.27, 82.88) [M::mem_pestat] low and high boundaries for proper pairs: (11, 781) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 41, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 586) [M::mem_pestat] mean and std.dev: (44.66, 54.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 775) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1139.616 CPU sec, 65.037 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 661951, 55, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (121, 671) [M::mem_pestat] mean and std.dev: (397.16, 82.84) [M::mem_pestat] low and high boundaries for proper pairs: (11, 781) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 38, 869) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2567) [M::mem_pestat] mean and std.dev: (413.88, 741.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3416) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1140.175 CPU sec, 50.461 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 661565, 63, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (121, 671) [M::mem_pestat] mean and std.dev: (397.28, 82.78) [M::mem_pestat] low and high boundaries for proper pairs: (11, 781) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 37, 72) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 184) [M::mem_pestat] mean and std.dev: (31.36, 24.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 240) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1148.167 CPU sec, 67.557 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 661900, 48, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.12, 82.79) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 29, 117) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 327) [M::mem_pestat] mean and std.dev: (40.66, 48.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 432) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1142.438 CPU sec, 50.542 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 661721, 44, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (270, 557, 2082) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5706) [M::mem_pestat] mean and std.dev: (981.30, 1167.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7518) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.09, 82.76) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 28, 47) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 113) [M::mem_pestat] mean and std.dev: (25.86, 16.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 146) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1149.380 CPU sec, 66.133 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 662125, 50, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (261, 425, 961) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2361) [M::mem_pestat] mean and std.dev: (558.70, 386.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3061) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (121, 671) [M::mem_pestat] mean and std.dev: (397.31, 82.98) [M::mem_pestat] low and high boundaries for proper pairs: (11, 781) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 33, 111) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 303) [M::mem_pestat] mean and std.dev: (37.26, 44.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 399) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1136.758 CPU sec, 50.392 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 662215, 36, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (170, 334, 723) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1829) [M::mem_pestat] mean and std.dev: (351.42, 234.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2382) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.19, 82.87) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 27, 100) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 276) [M::mem_pestat] mean and std.dev: (35.71, 41.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 364) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1140.515 CPU sec, 66.427 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 661883, 48, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (237, 301, 713) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1665) [M::mem_pestat] mean and std.dev: (409.00, 275.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2141) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (126, 666) [M::mem_pestat] mean and std.dev: (397.01, 82.60) [M::mem_pestat] low and high boundaries for proper pairs: (18, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 41, 547) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1605) [M::mem_pestat] mean and std.dev: (144.75, 329.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2134) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1135.707 CPU sec, 50.277 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 662116, 69, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (327, 496, 1186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2904) [M::mem_pestat] mean and std.dev: (599.54, 433.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3763) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (126, 666) [M::mem_pestat] mean and std.dev: (397.21, 82.62) [M::mem_pestat] low and high boundaries for proper pairs: (18, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 43, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603) [M::mem_pestat] mean and std.dev: (59.18, 77.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 797) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1139.698 CPU sec, 65.625 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 662391, 34, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (396.97, 82.74) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 37, 89) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 227) [M::mem_pestat] mean and std.dev: (32.56, 23.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 296) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1127.645 CPU sec, 49.871 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 662016, 58, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (121, 671) [M::mem_pestat] mean and std.dev: (397.26, 83.01) [M::mem_pestat] low and high boundaries for proper pairs: (11, 781) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 34, 62) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 154) [M::mem_pestat] mean and std.dev: (35.02, 29.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 200) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1139.901 CPU sec, 65.364 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 661449, 53, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (316, 444, 1278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3202) [M::mem_pestat] mean and std.dev: (754.60, 714.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4164) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (126, 666) [M::mem_pestat] mean and std.dev: (397.15, 82.72) [M::mem_pestat] low and high boundaries for proper pairs: (18, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 35, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650) [M::mem_pestat] mean and std.dev: (62.65, 110.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 860) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1141.531 CPU sec, 50.472 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 662436, 52, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (126, 666) [M::mem_pestat] mean and std.dev: (397.29, 82.62) [M::mem_pestat] low and high boundaries for proper pairs: (18, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 28, 794) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2352) [M::mem_pestat] mean and std.dev: (151.02, 419.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3131) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1135.213 CPU sec, 67.020 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 661622, 55, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (126, 666) [M::mem_pestat] mean and std.dev: (397.23, 82.70) [M::mem_pestat] low and high boundaries for proper pairs: (18, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (9, 30, 55) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 147) [M::mem_pestat] mean and std.dev: (29.22, 23.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 193) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1145.202 CPU sec, 66.656 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 661463, 63, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.17, 82.69) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 39, 455) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1337) [M::mem_pestat] mean and std.dev: (84.66, 192.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1778) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1133.328 CPU sec, 50.158 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 661804, 50, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (206, 344, 1119) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2945) [M::mem_pestat] mean and std.dev: (434.83, 404.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3858) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.20, 82.67) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 40, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1309) [M::mem_pestat] mean and std.dev: (82.72, 190.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1740) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1148.303 CPU sec, 66.663 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 661769, 59, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (258, 304, 1268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3288) [M::mem_pestat] mean and std.dev: (475.42, 417.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4298) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (126, 666) [M::mem_pestat] mean and std.dev: (397.22, 82.61) [M::mem_pestat] low and high boundaries for proper pairs: (18, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 27, 65) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 171) [M::mem_pestat] mean and std.dev: (28.50, 32.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 224) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1136.574 CPU sec, 50.266 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 662141, 53, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.20, 82.60) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 43, 1154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3430) [M::mem_pestat] mean and std.dev: (318.33, 676.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4568) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1137.672 CPU sec, 66.959 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 662116, 53, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.08, 82.68) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 35, 148) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402) [M::mem_pestat] mean and std.dev: (37.79, 35.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 529) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1130.298 CPU sec, 50.086 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 661361, 51, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (276, 348, 1578) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4182) [M::mem_pestat] mean and std.dev: (905.33, 1028.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5484) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (126, 666) [M::mem_pestat] mean and std.dev: (397.13, 82.62) [M::mem_pestat] low and high boundaries for proper pairs: (18, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 45, 629) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1839) [M::mem_pestat] mean and std.dev: (200.67, 405.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2444) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1147.424 CPU sec, 67.091 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 662114, 65, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (461, 475, 873) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1697) [M::mem_pestat] mean and std.dev: (544.20, 293.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2109) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (396.95, 82.66) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 38, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 566) [M::mem_pestat] mean and std.dev: (36.38, 42.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 750) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1131.039 CPU sec, 50.066 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 661610, 44, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (346, 643, 1233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3007) [M::mem_pestat] mean and std.dev: (777.90, 637.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3894) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (126, 666) [M::mem_pestat] mean and std.dev: (397.25, 82.62) [M::mem_pestat] low and high boundaries for proper pairs: (18, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 41, 1884) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5616) [M::mem_pestat] mean and std.dev: (504.56, 1013.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7482) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1140.166 CPU sec, 64.875 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 661982, 53, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (124, 295, 413) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 991) [M::mem_pestat] mean and std.dev: (215.38, 111.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1280) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (126, 666) [M::mem_pestat] mean and std.dev: (397.17, 82.50) [M::mem_pestat] low and high boundaries for proper pairs: (18, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 44, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 826) [M::mem_pestat] mean and std.dev: (102.98, 188.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1097) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1130.429 CPU sec, 50.017 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 662103, 50, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.01, 82.78) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (10, 25, 63) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 169) [M::mem_pestat] mean and std.dev: (27.67, 26.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 222) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1148.694 CPU sec, 66.813 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 661248, 51, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (159, 208, 1060) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2862) [M::mem_pestat] mean and std.dev: (436.62, 407.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3763) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.00, 82.80) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 34, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (41.95, 54.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 673) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1142.621 CPU sec, 50.490 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 662428, 48, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.20, 82.74) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 41, 137) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 373) [M::mem_pestat] mean and std.dev: (46.39, 47.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 491) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1136.097 CPU sec, 65.455 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 661745, 56, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 266, 391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1007) [M::mem_pestat] mean and std.dev: (237.89, 145.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1315) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.06, 82.65) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 47, 376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1088) [M::mem_pestat] mean and std.dev: (73.18, 138.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1444) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1139.355 CPU sec, 50.438 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 661542, 61, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.23, 82.81) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 29, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 534) [M::mem_pestat] mean and std.dev: (50.90, 74.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 708) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1137.659 CPU sec, 64.755 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 661904, 44, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.02, 82.69) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 39, 130) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 348) [M::mem_pestat] mean and std.dev: (49.24, 57.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 457) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1130.715 CPU sec, 50.069 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 662105, 54, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (126, 666) [M::mem_pestat] mean and std.dev: (397.19, 82.69) [M::mem_pestat] low and high boundaries for proper pairs: (18, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 47, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 759) [M::mem_pestat] mean and std.dev: (50.76, 57.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1005) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1129.255 CPU sec, 64.613 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 661735, 59, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.30, 82.73) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 42, 746) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2204) [M::mem_pestat] mean and std.dev: (331.04, 613.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2933) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1132.002 CPU sec, 50.112 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 661925, 47, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (284, 302, 872) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2048) [M::mem_pestat] mean and std.dev: (385.11, 219.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2636) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.21, 82.71) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 27, 66) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 166) [M::mem_pestat] mean and std.dev: (32.61, 27.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 216) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1128.988 CPU sec, 64.524 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 662389, 53, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (282, 323, 2557) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7107) [M::mem_pestat] mean and std.dev: (1296.64, 1567.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9382) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.20, 82.70) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 50, 1366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4058) [M::mem_pestat] mean and std.dev: (396.48, 760.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5404) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1123.705 CPU sec, 49.555 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 662041, 55, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.22, 82.76) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 37, 1177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3495) [M::mem_pestat] mean and std.dev: (517.54, 936.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4654) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1137.004 CPU sec, 65.089 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 661757, 50, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.10, 82.75) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 32, 56) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 138) [M::mem_pestat] mean and std.dev: (28.27, 18.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 179) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1130.178 CPU sec, 49.967 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 661956, 49, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.17, 82.74) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 26, 1168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3474) [M::mem_pestat] mean and std.dev: (346.30, 785.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4627) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1128.357 CPU sec, 63.057 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 662145, 47, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (224, 329, 875) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2177) [M::mem_pestat] mean and std.dev: (488.90, 437.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2828) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.13, 82.68) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 42, 96) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 252) [M::mem_pestat] mean and std.dev: (45.49, 46.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 330) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1125.932 CPU sec, 49.760 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 661516, 42, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (219, 411, 487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1023) [M::mem_pestat] mean and std.dev: (300.18, 148.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1291) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.25, 82.74) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 33, 128) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 344) [M::mem_pestat] mean and std.dev: (56.45, 55.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 452) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1131.086 CPU sec, 62.192 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 661514, 58, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (276, 458, 1449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3795) [M::mem_pestat] mean and std.dev: (834.30, 770.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4968) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (126, 666) [M::mem_pestat] mean and std.dev: (397.10, 82.68) [M::mem_pestat] low and high boundaries for proper pairs: (18, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 34, 72) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 182) [M::mem_pestat] mean and std.dev: (34.34, 35.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 237) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1129.398 CPU sec, 49.830 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 661885, 38, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (126, 666) [M::mem_pestat] mean and std.dev: (397.13, 82.65) [M::mem_pestat] low and high boundaries for proper pairs: (18, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 39, 63) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 157) [M::mem_pestat] mean and std.dev: (34.84, 30.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 204) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1129.506 CPU sec, 64.234 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 661992, 59, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (288, 458, 877) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2055) [M::mem_pestat] mean and std.dev: (559.78, 566.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2826) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.27, 82.81) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 43, 124) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 318) [M::mem_pestat] mean and std.dev: (41.38, 32.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 415) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1121.114 CPU sec, 49.529 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 662283, 45, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (190, 360, 515) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1165) [M::mem_pestat] mean and std.dev: (290.80, 151.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1490) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.18, 82.83) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 46, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631) [M::mem_pestat] mean and std.dev: (61.06, 92.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 835) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1126.895 CPU sec, 64.952 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 662556, 57, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.03, 82.87) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 47, 1702) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5062) [M::mem_pestat] mean and std.dev: (634.66, 1177.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6742) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1133.741 CPU sec, 66.799 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 662117, 55, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.15, 82.71) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 38, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 527) [M::mem_pestat] mean and std.dev: (38.47, 43.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 697) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1129.277 CPU sec, 49.984 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 662027, 45, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (137, 388, 975) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2651) [M::mem_pestat] mean and std.dev: (460.69, 391.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3489) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (126, 666) [M::mem_pestat] mean and std.dev: (397.15, 82.60) [M::mem_pestat] low and high boundaries for proper pairs: (18, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 31, 133) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 367) [M::mem_pestat] mean and std.dev: (53.36, 75.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 484) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1128.181 CPU sec, 65.913 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 661988, 57, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (282, 341, 949) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2283) [M::mem_pestat] mean and std.dev: (507.20, 361.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2950) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.22, 82.69) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 36, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 988) [M::mem_pestat] mean and std.dev: (102.69, 180.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1312) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1124.742 CPU sec, 49.664 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 662264, 53, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (306, 717, 1166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2886) [M::mem_pestat] mean and std.dev: (797.67, 695.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3746) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (126, 666) [M::mem_pestat] mean and std.dev: (397.09, 82.53) [M::mem_pestat] low and high boundaries for proper pairs: (18, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 38, 76) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 198) [M::mem_pestat] mean and std.dev: (37.75, 36.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 259) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1123.756 CPU sec, 64.090 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 662021, 53, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (246, 366, 667) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1509) [M::mem_pestat] mean and std.dev: (432.00, 304.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1930) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.11, 82.81) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (10, 29, 119) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 337) [M::mem_pestat] mean and std.dev: (27.83, 31.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 446) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1128.430 CPU sec, 49.936 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 661640, 52, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (197, 342, 474) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1028) [M::mem_pestat] mean and std.dev: (314.50, 138.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1305) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (126, 666) [M::mem_pestat] mean and std.dev: (397.21, 82.53) [M::mem_pestat] low and high boundaries for proper pairs: (18, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 30, 71) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 179) [M::mem_pestat] mean and std.dev: (30.60, 25.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 233) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1121.915 CPU sec, 63.737 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 662178, 50, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (126, 666) [M::mem_pestat] mean and std.dev: (397.30, 82.66) [M::mem_pestat] low and high boundaries for proper pairs: (18, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (11, 35, 114) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 320) [M::mem_pestat] mean and std.dev: (33.98, 36.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 423) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1118.994 CPU sec, 49.412 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 661548, 54, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (205, 370, 686) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1648) [M::mem_pestat] mean and std.dev: (353.78, 228.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2129) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.33, 82.84) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 47, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 854) [M::mem_pestat] mean and std.dev: (69.09, 92.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1133) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1124.211 CPU sec, 63.547 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 661626, 41, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (121, 671) [M::mem_pestat] mean and std.dev: (397.33, 82.87) [M::mem_pestat] low and high boundaries for proper pairs: (11, 781) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 43, 1583) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4713) [M::mem_pestat] mean and std.dev: (677.71, 1248.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6278) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1123.600 CPU sec, 49.604 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 661683, 58, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (396.95, 82.66) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 36, 682) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2006) [M::mem_pestat] mean and std.dev: (177.63, 415.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2668) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1127.270 CPU sec, 64.216 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 662317, 70, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (284, 485, 863) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2021) [M::mem_pestat] mean and std.dev: (416.10, 228.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2600) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.16, 82.76) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 48, 1770) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5274) [M::mem_pestat] mean and std.dev: (781.52, 1365.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7026) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1125.254 CPU sec, 49.634 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 662218, 52, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (328, 410, 3063) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8533) [M::mem_pestat] mean and std.dev: (1160.23, 1400.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11268) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (121, 671) [M::mem_pestat] mean and std.dev: (397.09, 82.95) [M::mem_pestat] low and high boundaries for proper pairs: (11, 781) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 41, 130) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 358) [M::mem_pestat] mean and std.dev: (40.86, 41.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 472) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1126.204 CPU sec, 64.627 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 661835, 49, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.02, 82.66) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 36, 97) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 261) [M::mem_pestat] mean and std.dev: (33.13, 28.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 343) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1123.890 CPU sec, 49.781 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 662468, 55, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.14, 82.71) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 35, 130) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 358) [M::mem_pestat] mean and std.dev: (49.52, 69.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 472) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1128.283 CPU sec, 63.811 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 661941, 43, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (396.97, 82.71) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 39, 1173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3475) [M::mem_pestat] mean and std.dev: (431.82, 870.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4626) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1123.238 CPU sec, 49.743 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 662022, 54, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.19, 82.83) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 38, 129) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 363) [M::mem_pestat] mean and std.dev: (40.80, 46.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 480) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1126.606 CPU sec, 64.652 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 662249, 53, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (244, 319, 1020) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2572) [M::mem_pestat] mean and std.dev: (503.00, 543.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3348) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (126, 666) [M::mem_pestat] mean and std.dev: (397.22, 82.60) [M::mem_pestat] low and high boundaries for proper pairs: (18, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 29, 1277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3803) [M::mem_pestat] mean and std.dev: (402.08, 824.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5066) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1120.993 CPU sec, 49.636 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 661255, 43, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.11, 82.81) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 40, 90) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 234) [M::mem_pestat] mean and std.dev: (33.32, 23.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 306) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1125.554 CPU sec, 63.088 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 662530, 61, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (126, 666) [M::mem_pestat] mean and std.dev: (397.11, 82.54) [M::mem_pestat] low and high boundaries for proper pairs: (18, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 39, 130) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 354) [M::mem_pestat] mean and std.dev: (45.84, 53.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 466) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1117.868 CPU sec, 49.342 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 661682, 50, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.25, 82.70) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 42, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 518) [M::mem_pestat] mean and std.dev: (54.35, 77.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 685) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1128.858 CPU sec, 63.790 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 662278, 60, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.29, 82.74) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 28, 59) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 149) [M::mem_pestat] mean and std.dev: (28.86, 22.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 194) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1124.834 CPU sec, 49.739 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 661685, 43, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (247, 383, 487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 967) [M::mem_pestat] mean and std.dev: (294.25, 145.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1207) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.19, 82.71) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 24, 90) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 240) [M::mem_pestat] mean and std.dev: (36.50, 36.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 315) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1128.747 CPU sec, 63.980 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 661434, 60, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (265, 384, 881) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2113) [M::mem_pestat] mean and std.dev: (522.80, 356.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2729) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.05, 82.59) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 39, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 877) [M::mem_pestat] mean and std.dev: (70.88, 117.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1165) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1127.784 CPU sec, 49.729 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 661439, 41, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (255, 324, 996) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2478) [M::mem_pestat] mean and std.dev: (369.55, 279.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3219) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (396.99, 82.73) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 22, 39) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 91) [M::mem_pestat] mean and std.dev: (22.60, 17.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 117) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1130.438 CPU sec, 64.266 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 661688, 44, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (308, 593, 2125) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5759) [M::mem_pestat] mean and std.dev: (1151.92, 1149.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7576) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.39, 82.81) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 43, 979) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2905) [M::mem_pestat] mean and std.dev: (386.32, 685.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3868) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1126.773 CPU sec, 49.788 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 661575, 54, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (396.95, 82.75) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 35, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (30.85, 29.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 666) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1130.297 CPU sec, 63.845 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 661898, 48, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (261, 340, 577) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1209) [M::mem_pestat] mean and std.dev: (430.09, 303.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1644) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (121, 671) [M::mem_pestat] mean and std.dev: (397.26, 82.91) [M::mem_pestat] low and high boundaries for proper pairs: (11, 781) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 38, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 756) [M::mem_pestat] mean and std.dev: (84.20, 144.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1003) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1131.038 CPU sec, 49.958 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 662221, 58, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (158, 284, 378) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 818) [M::mem_pestat] mean and std.dev: (216.50, 115.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1038) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.12, 82.75) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 40, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615) [M::mem_pestat] mean and std.dev: (51.09, 85.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 816) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1141.927 CPU sec, 65.819 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 661512, 54, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.12, 82.83) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 45, 1023) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3023) [M::mem_pestat] mean and std.dev: (378.43, 772.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4023) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1130.294 CPU sec, 65.261 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 661525, 46, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (351, 688, 1368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3402) [M::mem_pestat] mean and std.dev: (833.80, 610.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4419) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.15, 82.73) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (11, 34, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497) [M::mem_pestat] mean and std.dev: (54.76, 93.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 659) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1130.457 CPU sec, 50.100 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 662721, 48, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (126, 666) [M::mem_pestat] mean and std.dev: (397.09, 82.51) [M::mem_pestat] low and high boundaries for proper pairs: (18, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 40, 150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (41.74, 46.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 546) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1128.214 CPU sec, 64.489 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 661258, 46, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (258, 324, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813) [M::mem_pestat] mean and std.dev: (295.80, 106.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 998) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.15, 82.80) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 24, 71) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 187) [M::mem_pestat] mean and std.dev: (36.42, 40.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 245) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1131.784 CPU sec, 50.062 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 661850, 61, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.01, 82.74) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 42, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748) [M::mem_pestat] mean and std.dev: (63.65, 90.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 991) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1128.449 CPU sec, 63.865 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 662410, 46, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (269, 519, 1157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2933) [M::mem_pestat] mean and std.dev: (652.36, 610.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3821) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (126, 666) [M::mem_pestat] mean and std.dev: (397.24, 82.58) [M::mem_pestat] low and high boundaries for proper pairs: (18, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (9, 35, 729) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2169) [M::mem_pestat] mean and std.dev: (147.05, 379.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2889) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1118.691 CPU sec, 49.350 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 662515, 53, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.03, 82.68) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (11, 27, 139) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 395) [M::mem_pestat] mean and std.dev: (33.69, 39.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 523) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1123.044 CPU sec, 63.724 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 661479, 40, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 323, 378) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 796) [M::mem_pestat] mean and std.dev: (239.88, 102.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1005) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (121, 671) [M::mem_pestat] mean and std.dev: (397.25, 82.91) [M::mem_pestat] low and high boundaries for proper pairs: (11, 781) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (11, 25, 60) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 158) [M::mem_pestat] mean and std.dev: (26.97, 27.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 207) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1130.103 CPU sec, 49.875 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 661603, 53, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (121, 671) [M::mem_pestat] mean and std.dev: (397.27, 82.94) [M::mem_pestat] low and high boundaries for proper pairs: (11, 781) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 50, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 724) [M::mem_pestat] mean and std.dev: (70.74, 104.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 957) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1124.749 CPU sec, 63.782 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 661668, 55, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (217, 368, 1722) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4732) [M::mem_pestat] mean and std.dev: (719.90, 726.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6237) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.06, 82.69) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 32, 793) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2351) [M::mem_pestat] mean and std.dev: (148.72, 374.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3130) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1122.457 CPU sec, 49.605 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 662188, 52, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.18, 82.71) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 49, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 833) [M::mem_pestat] mean and std.dev: (71.10, 130.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1105) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1126.242 CPU sec, 65.704 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 662306, 53, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.06, 82.67) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 35, 492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1444) [M::mem_pestat] mean and std.dev: (104.35, 235.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1920) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1126.562 CPU sec, 49.864 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 661778, 57, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (389, 948, 1611) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4055) [M::mem_pestat] mean and std.dev: (1164.67, 1028.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5278) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.35, 82.74) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 40, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570) [M::mem_pestat] mean and std.dev: (55.68, 77.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 756) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1123.944 CPU sec, 62.995 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 661869, 57, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (126, 666) [M::mem_pestat] mean and std.dev: (397.23, 82.65) [M::mem_pestat] low and high boundaries for proper pairs: (18, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 27, 77) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 205) [M::mem_pestat] mean and std.dev: (29.60, 24.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 269) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1122.082 CPU sec, 49.544 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 662393, 50, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (218, 327, 913) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2303) [M::mem_pestat] mean and std.dev: (377.83, 276.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2998) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.11, 82.80) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 32, 72) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 186) [M::mem_pestat] mean and std.dev: (28.77, 21.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 243) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1111, 1423, 1466) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (401, 2176) [M::mem_pestat] mean and std.dev: (1211.71, 337.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2561) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1119.307 CPU sec, 63.298 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 661771, 53, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.06, 82.75) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 39, 89) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 237) [M::mem_pestat] mean and std.dev: (33.76, 27.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 311) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1114.160 CPU sec, 49.343 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 662317, 55, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.09, 82.78) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 42, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 822) [M::mem_pestat] mean and std.dev: (97.00, 183.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1090) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1118.641 CPU sec, 64.127 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 661863, 61, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.12, 82.60) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 39, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936) [M::mem_pestat] mean and std.dev: (68.39, 125.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1240) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1126.506 CPU sec, 49.708 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 661885, 55, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (275, 580, 722) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1616) [M::mem_pestat] mean and std.dev: (535.55, 331.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2063) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (126, 666) [M::mem_pestat] mean and std.dev: (397.19, 82.56) [M::mem_pestat] low and high boundaries for proper pairs: (18, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 75, 2032) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6042) [M::mem_pestat] mean and std.dev: (879.25, 1374.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8047) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1121.792 CPU sec, 64.465 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 661861, 50, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.20, 82.76) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 48, 406) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1172) [M::mem_pestat] mean and std.dev: (148.42, 282.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1555) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1128.844 CPU sec, 49.819 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 662177, 44, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (121, 671) [M::mem_pestat] mean and std.dev: (397.29, 82.86) [M::mem_pestat] low and high boundaries for proper pairs: (11, 781) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 48, 125) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 329) [M::mem_pestat] mean and std.dev: (47.44, 39.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 431) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1129.977 CPU sec, 66.203 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 662270, 60, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 172, 628) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1728) [M::mem_pestat] mean and std.dev: (362.35, 391.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2278) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.06, 82.67) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 54, 1222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3620) [M::mem_pestat] mean and std.dev: (465.15, 863.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4819) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1131.144 CPU sec, 50.100 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 662125, 43, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.07, 82.68) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (11, 27, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953) [M::mem_pestat] mean and std.dev: (48.35, 116.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1267) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1127.346 CPU sec, 64.349 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 661521, 54, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (121, 671) [M::mem_pestat] mean and std.dev: (397.28, 82.91) [M::mem_pestat] low and high boundaries for proper pairs: (11, 781) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 42, 396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1160) [M::mem_pestat] mean and std.dev: (104.52, 229.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1542) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1124.366 CPU sec, 49.657 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 661911, 57, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.15, 82.76) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 39, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903) [M::mem_pestat] mean and std.dev: (39.68, 53.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1199) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1117.545 CPU sec, 64.414 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 661825, 69, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.19, 82.80) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 31, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 851) [M::mem_pestat] mean and std.dev: (87.57, 183.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1130) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1118.379 CPU sec, 49.403 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 661558, 46, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (121, 671) [M::mem_pestat] mean and std.dev: (397.22, 83.05) [M::mem_pestat] low and high boundaries for proper pairs: (11, 781) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 49, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (47.00, 48.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 619) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1124.203 CPU sec, 63.677 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 661496, 47, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (131, 236, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (194.36, 119.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 672) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (126, 666) [M::mem_pestat] mean and std.dev: (397.27, 82.63) [M::mem_pestat] low and high boundaries for proper pairs: (18, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 39, 73) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 187) [M::mem_pestat] mean and std.dev: (32.42, 22.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 244) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1129.031 CPU sec, 49.872 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 662109, 53, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.19, 82.66) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 41, 376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1104) [M::mem_pestat] mean and std.dev: (91.68, 189.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1468) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1135.818 CPU sec, 64.183 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 661467, 75, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.11, 82.85) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 37, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763) [M::mem_pestat] mean and std.dev: (56.07, 100.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1011) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1135.222 CPU sec, 65.056 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 662830, 41, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (283, 331, 545) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1069) [M::mem_pestat] mean and std.dev: (316.12, 125.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1331) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.09, 82.76) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 41, 1859) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5551) [M::mem_pestat] mean and std.dev: (699.86, 1299.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7397) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1120.843 CPU sec, 49.469 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 662133, 62, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.20, 82.67) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 41, 107) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 283) [M::mem_pestat] mean and std.dev: (41.22, 38.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 371) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1120.331 CPU sec, 63.385 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 662395, 50, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.23, 82.80) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 34, 54) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 132) [M::mem_pestat] mean and std.dev: (33.37, 27.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 171) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1117.407 CPU sec, 49.331 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 661941, 57, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.05, 82.80) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 33, 121) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 321) [M::mem_pestat] mean and std.dev: (35.74, 35.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 421) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1126.948 CPU sec, 64.235 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 661878, 62, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.28, 82.84) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 46, 888) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2638) [M::mem_pestat] mean and std.dev: (279.83, 606.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3513) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1119.721 CPU sec, 49.416 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 661519, 50, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (126, 666) [M::mem_pestat] mean and std.dev: (397.10, 82.63) [M::mem_pestat] low and high boundaries for proper pairs: (18, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 35, 152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420) [M::mem_pestat] mean and std.dev: (48.46, 69.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 554) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1128.857 CPU sec, 63.315 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 662064, 59, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.13, 82.73) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 38, 139) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 373) [M::mem_pestat] mean and std.dev: (40.91, 43.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 490) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1123.333 CPU sec, 49.662 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 661895, 65, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (255, 286, 1235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3195) [M::mem_pestat] mean and std.dev: (384.89, 338.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4175) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (126, 666) [M::mem_pestat] mean and std.dev: (397.32, 82.62) [M::mem_pestat] low and high boundaries for proper pairs: (18, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 33, 152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (38.29, 48.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 548) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1121.623 CPU sec, 64.200 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 662097, 56, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.18, 82.84) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 37, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609) [M::mem_pestat] mean and std.dev: (34.93, 39.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 807) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1121.035 CPU sec, 49.597 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 661958, 50, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (270, 336, 1157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2931) [M::mem_pestat] mean and std.dev: (559.08, 464.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3818) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (396.95, 82.72) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 33, 92) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 248) [M::mem_pestat] mean and std.dev: (39.60, 43.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 326) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1123.534 CPU sec, 63.708 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 662830, 54, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (383, 621, 1010) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2264) [M::mem_pestat] mean and std.dev: (535.62, 301.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2891) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.05, 82.64) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 30, 108) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 296) [M::mem_pestat] mean and std.dev: (33.64, 33.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 390) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1112.947 CPU sec, 49.225 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 662232, 66, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (231, 287, 402) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744) [M::mem_pestat] mean and std.dev: (255.64, 111.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 915) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.30, 82.80) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 34, 821) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2437) [M::mem_pestat] mean and std.dev: (287.60, 579.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3245) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1125.849 CPU sec, 63.314 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 662263, 53, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (126, 666) [M::mem_pestat] mean and std.dev: (397.20, 82.56) [M::mem_pestat] low and high boundaries for proper pairs: (18, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 36, 122) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 332) [M::mem_pestat] mean and std.dev: (43.70, 49.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 437) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1119.526 CPU sec, 49.438 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 661634, 62, 10) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (121, 671) [M::mem_pestat] mean and std.dev: (397.19, 82.88) [M::mem_pestat] low and high boundaries for proper pairs: (11, 781) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 38, 111) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 291) [M::mem_pestat] mean and std.dev: (38.53, 32.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 381) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (507, 1100, 1586) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3744) [M::mem_pestat] mean and std.dev: (1068.60, 702.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4823) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1127.191 CPU sec, 63.480 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 662323, 50, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (197, 274, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 884) [M::mem_pestat] mean and std.dev: (304.70, 171.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1113) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.19, 82.81) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 46, 1611) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4799) [M::mem_pestat] mean and std.dev: (712.67, 1257.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6393) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1123.103 CPU sec, 49.673 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 661927, 52, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.06, 82.75) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 56, 151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (46.80, 44.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 541) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1126.657 CPU sec, 64.201 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 661888, 52, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (269, 529, 1109) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2789) [M::mem_pestat] mean and std.dev: (765.28, 651.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3629) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.08, 82.66) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 44, 1150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3410) [M::mem_pestat] mean and std.dev: (378.00, 748.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4540) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1124.819 CPU sec, 49.751 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 662396, 43, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (215, 558, 945) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2405) [M::mem_pestat] mean and std.dev: (539.10, 424.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3135) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.16, 82.78) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (9, 41, 859) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2559) [M::mem_pestat] mean and std.dev: (230.57, 489.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3409) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1121.711 CPU sec, 63.414 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 661966, 52, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.08, 82.75) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 35, 119) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 327) [M::mem_pestat] mean and std.dev: (44.21, 42.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 431) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1126.602 CPU sec, 49.774 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 662386, 52, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (126, 666) [M::mem_pestat] mean and std.dev: (397.07, 82.49) [M::mem_pestat] low and high boundaries for proper pairs: (18, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 34, 153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (50.42, 69.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 549) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1130.374 CPU sec, 64.643 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 661903, 66, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.01, 82.65) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 37, 110) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 306) [M::mem_pestat] mean and std.dev: (40.72, 46.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 404) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1129.234 CPU sec, 50.017 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 661662, 62, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (235, 293, 762) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1816) [M::mem_pestat] mean and std.dev: (459.18, 410.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2343) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.04, 82.67) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (10, 27, 66) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 178) [M::mem_pestat] mean and std.dev: (29.13, 26.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 234) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1125.883 CPU sec, 64.711 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 661788, 52, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (244, 337, 406) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 730) [M::mem_pestat] mean and std.dev: (303.60, 109.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (126, 666) [M::mem_pestat] mean and std.dev: (397.18, 82.65) [M::mem_pestat] low and high boundaries for proper pairs: (18, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 31, 1752) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5226) [M::mem_pestat] mean and std.dev: (707.31, 1222.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6963) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1123.353 CPU sec, 49.710 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 661954, 69, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.34, 82.79) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 38, 560) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1654) [M::mem_pestat] mean and std.dev: (180.79, 373.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2201) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1122.249 CPU sec, 62.898 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 662177, 61, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (121, 671) [M::mem_pestat] mean and std.dev: (397.39, 82.86) [M::mem_pestat] low and high boundaries for proper pairs: (11, 781) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 29, 392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1148) [M::mem_pestat] mean and std.dev: (62.83, 117.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1526) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1120.363 CPU sec, 49.581 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 662098, 53, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (126, 666) [M::mem_pestat] mean and std.dev: (397.09, 82.54) [M::mem_pestat] low and high boundaries for proper pairs: (18, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 30, 1161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3451) [M::mem_pestat] mean and std.dev: (307.32, 686.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4596) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1134.411 CPU sec, 65.003 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 661674, 49, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.20, 82.91) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 46, 1045) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3089) [M::mem_pestat] mean and std.dev: (247.10, 539.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4111) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1128.559 CPU sec, 49.931 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 661836, 64, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.11, 82.83) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 35, 497) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1451) [M::mem_pestat] mean and std.dev: (98.73, 235.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1928) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1132.092 CPU sec, 62.977 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 661986, 58, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (209, 299, 348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 626) [M::mem_pestat] mean and std.dev: (258.80, 123.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 765) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.16, 82.72) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 43, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 535) [M::mem_pestat] mean and std.dev: (42.45, 41.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 706) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1133.712 CPU sec, 65.319 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 662315, 58, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.04, 82.65) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 44, 90) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 234) [M::mem_pestat] mean and std.dev: (34.04, 24.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 306) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1126.914 CPU sec, 49.721 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 661857, 47, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.07, 82.78) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 43, 117) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 311) [M::mem_pestat] mean and std.dev: (56.79, 66.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 408) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1129.942 CPU sec, 65.076 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 662166, 59, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (126, 666) [M::mem_pestat] mean and std.dev: (397.19, 82.62) [M::mem_pestat] low and high boundaries for proper pairs: (18, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 34, 71) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 177) [M::mem_pestat] mean and std.dev: (34.94, 28.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 230) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1119.091 CPU sec, 49.489 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 662218, 53, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.20, 82.71) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 39, 116) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 306) [M::mem_pestat] mean and std.dev: (41.33, 40.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 401) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1118.999 CPU sec, 64.108 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 662515, 64, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (272, 334, 1070) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2666) [M::mem_pestat] mean and std.dev: (603.45, 523.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3464) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (126, 666) [M::mem_pestat] mean and std.dev: (397.20, 82.65) [M::mem_pestat] low and high boundaries for proper pairs: (18, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 76, 2290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6830) [M::mem_pestat] mean and std.dev: (1014.13, 1703.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9100) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1125.767 CPU sec, 50.019 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 662227, 56, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.01, 82.73) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 36, 107) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 285) [M::mem_pestat] mean and std.dev: (49.12, 58.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 374) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1127.621 CPU sec, 63.393 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 661706, 55, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (126, 666) [M::mem_pestat] mean and std.dev: (397.19, 82.58) [M::mem_pestat] low and high boundaries for proper pairs: (18, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 31, 63) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 163) [M::mem_pestat] mean and std.dev: (28.77, 27.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 213) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1133.401 CPU sec, 50.274 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 662455, 43, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.15, 82.75) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 33, 158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 440) [M::mem_pestat] mean and std.dev: (36.24, 35.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 581) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1126.519 CPU sec, 64.218 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 661689, 62, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.17, 82.78) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 39, 462) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1350) [M::mem_pestat] mean and std.dev: (81.57, 198.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1794) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1136.069 CPU sec, 50.553 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 661943, 50, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (202, 242, 731) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1789) [M::mem_pestat] mean and std.dev: (342.30, 287.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2318) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.20, 82.68) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 40, 650) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1922) [M::mem_pestat] mean and std.dev: (211.63, 438.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2558) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1147.839 CPU sec, 67.444 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 661788, 50, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (285, 316, 1554) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4092) [M::mem_pestat] mean and std.dev: (740.14, 652.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5361) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (121, 671) [M::mem_pestat] mean and std.dev: (397.34, 82.96) [M::mem_pestat] low and high boundaries for proper pairs: (11, 781) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 39, 1968) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5872) [M::mem_pestat] mean and std.dev: (638.91, 1281.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7824) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1128.298 CPU sec, 49.888 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 661692, 60, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.10, 82.84) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 52, 1603) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4773) [M::mem_pestat] mean and std.dev: (664.88, 1195.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6358) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1144.622 CPU sec, 65.098 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 662151, 61, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (126, 666) [M::mem_pestat] mean and std.dev: (397.14, 82.68) [M::mem_pestat] low and high boundaries for proper pairs: (18, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 43, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1241) [M::mem_pestat] mean and std.dev: (121.35, 210.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1647) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1133.280 CPU sec, 50.249 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 661746, 44, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (167, 324, 1040) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2786) [M::mem_pestat] mean and std.dev: (308.55, 258.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3659) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (121, 671) [M::mem_pestat] mean and std.dev: (397.32, 82.99) [M::mem_pestat] low and high boundaries for proper pairs: (11, 781) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (9, 40, 336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 990) [M::mem_pestat] mean and std.dev: (41.00, 63.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1317) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1133.214 CPU sec, 66.406 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 661714, 48, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (260, 1243, 3640) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10400) [M::mem_pestat] mean and std.dev: (2184.20, 2593.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13780) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (121, 671) [M::mem_pestat] mean and std.dev: (397.32, 82.95) [M::mem_pestat] low and high boundaries for proper pairs: (11, 781) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 28, 75) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 199) [M::mem_pestat] mean and std.dev: (26.50, 21.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 261) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1134.679 CPU sec, 50.399 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 661684, 46, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (225, 318, 1690) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4620) [M::mem_pestat] mean and std.dev: (797.00, 844.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6085) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.13, 82.83) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 36, 487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1429) [M::mem_pestat] mean and std.dev: (120.37, 278.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1900) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1132.912 CPU sec, 64.783 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 661597, 60, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (284, 395, 670) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1442) [M::mem_pestat] mean and std.dev: (455.60, 365.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1917) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.15, 82.68) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 34, 125) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 337) [M::mem_pestat] mean and std.dev: (43.92, 59.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 443) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1132.421 CPU sec, 50.166 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 662302, 67, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (207, 311, 857) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2157) [M::mem_pestat] mean and std.dev: (475.00, 500.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2807) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.11, 82.71) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 41, 105) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 275) [M::mem_pestat] mean and std.dev: (45.05, 48.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 360) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1131.812 CPU sec, 66.607 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 661534, 55, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.26, 82.77) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 33, 1123) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3331) [M::mem_pestat] mean and std.dev: (258.24, 571.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4435) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1143.055 CPU sec, 64.259 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 661684, 55, 10) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (126, 666) [M::mem_pestat] mean and std.dev: (397.21, 82.65) [M::mem_pestat] low and high boundaries for proper pairs: (18, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 37, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609) [M::mem_pestat] mean and std.dev: (48.63, 65.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 805) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (213, 1065, 1627) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4455) [M::mem_pestat] mean and std.dev: (1099.40, 1071.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5869) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1128.899 CPU sec, 49.992 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 661967, 45, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.04, 82.74) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 33, 55) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 121) [M::mem_pestat] mean and std.dev: (32.63, 23.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 154) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1145.908 CPU sec, 65.876 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 662184, 51, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (264, 346, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 783) [M::mem_pestat] mean and std.dev: (290.50, 153.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 956) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.36, 82.80) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 40, 837) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2483) [M::mem_pestat] mean and std.dev: (172.86, 395.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3306) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1133.966 CPU sec, 50.087 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 661759, 57, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.14, 82.67) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 26, 336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 982) [M::mem_pestat] mean and std.dev: (62.96, 129.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1305) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1140.668 CPU sec, 66.244 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 661612, 55, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (262, 292, 744) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1708) [M::mem_pestat] mean and std.dev: (384.70, 245.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2190) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (121, 671) [M::mem_pestat] mean and std.dev: (397.23, 82.86) [M::mem_pestat] low and high boundaries for proper pairs: (11, 781) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 43, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1020) [M::mem_pestat] mean and std.dev: (49.69, 66.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1354) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1137.427 CPU sec, 50.451 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 662132, 47, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.13, 82.83) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 35, 91) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 235) [M::mem_pestat] mean and std.dev: (32.21, 25.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 307) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1140.421 CPU sec, 65.872 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 662161, 59, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (121, 671) [M::mem_pestat] mean and std.dev: (397.16, 82.94) [M::mem_pestat] low and high boundaries for proper pairs: (11, 781) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 35, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922) [M::mem_pestat] mean and std.dev: (54.91, 98.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1225) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1134.400 CPU sec, 50.234 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 661761, 50, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (121, 671) [M::mem_pestat] mean and std.dev: (397.16, 82.97) [M::mem_pestat] low and high boundaries for proper pairs: (11, 781) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 37, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1249) [M::mem_pestat] mean and std.dev: (142.30, 278.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1660) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1150.842 CPU sec, 66.903 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 661837, 55, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (237, 348, 497) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1017) [M::mem_pestat] mean and std.dev: (264.50, 146.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1277) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.16, 82.76) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 33, 86) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 214) [M::mem_pestat] mean and std.dev: (39.04, 36.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 278) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1136.276 CPU sec, 50.379 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 661953, 55, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (228, 311, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 870) [M::mem_pestat] mean and std.dev: (286.69, 133.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1084) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.39, 82.80) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 30, 77) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 195) [M::mem_pestat] mean and std.dev: (36.02, 37.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 254) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1147.194 CPU sec, 66.415 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 661688, 51, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.08, 82.58) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (10, 23, 49) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 127) [M::mem_pestat] mean and std.dev: (28.89, 30.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 166) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1136.453 CPU sec, 50.421 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 661781, 52, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (237, 474, 1088) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2790) [M::mem_pestat] mean and std.dev: (688.10, 612.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3641) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.15, 82.69) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 30, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (37.83, 40.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 787) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1139.795 CPU sec, 65.962 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 662032, 62, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (162, 390, 717) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1827) [M::mem_pestat] mean and std.dev: (309.62, 191.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2382) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (126, 666) [M::mem_pestat] mean and std.dev: (397.22, 82.63) [M::mem_pestat] low and high boundaries for proper pairs: (18, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 34, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 560) [M::mem_pestat] mean and std.dev: (43.51, 76.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 742) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1130.699 CPU sec, 50.149 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 662077, 59, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.08, 82.81) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 36, 1265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3767) [M::mem_pestat] mean and std.dev: (469.93, 909.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5018) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1145.073 CPU sec, 66.737 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 661440, 49, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (226, 381, 1354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3610) [M::mem_pestat] mean and std.dev: (484.50, 419.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4738) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (121, 671) [M::mem_pestat] mean and std.dev: (397.36, 82.89) [M::mem_pestat] low and high boundaries for proper pairs: (11, 781) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (11, 36, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479) [M::mem_pestat] mean and std.dev: (36.92, 42.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1146.647 CPU sec, 50.841 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 661728, 71, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.01, 82.79) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 40, 672) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1982) [M::mem_pestat] mean and std.dev: (122.32, 303.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2637) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1143.500 CPU sec, 65.821 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 662218, 59, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.23, 82.73) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 38, 94) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 244) [M::mem_pestat] mean and std.dev: (42.92, 40.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 319) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1134.323 CPU sec, 50.172 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 662073, 57, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.18, 82.81) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 36, 72) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 172) [M::mem_pestat] mean and std.dev: (40.56, 33.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 222) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1136.076 CPU sec, 65.523 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 662248, 51, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (126, 666) [M::mem_pestat] mean and std.dev: (397.20, 82.74) [M::mem_pestat] low and high boundaries for proper pairs: (18, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 35, 146) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402) [M::mem_pestat] mean and std.dev: (45.85, 64.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 530) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1135.505 CPU sec, 50.177 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 662731, 43, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (294, 337, 523) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 981) [M::mem_pestat] mean and std.dev: (323.78, 99.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1210) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.10, 82.75) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 31, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707) [M::mem_pestat] mean and std.dev: (47.82, 65.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 938) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1138.591 CPU sec, 66.514 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 661852, 72, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (145, 646, 1273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3529) [M::mem_pestat] mean and std.dev: (756.73, 617.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4657) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.13, 82.66) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 32, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909) [M::mem_pestat] mean and std.dev: (80.14, 169.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1207) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1134.792 CPU sec, 50.148 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 661990, 57, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.22, 82.71) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 46, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 522) [M::mem_pestat] mean and std.dev: (46.91, 41.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 689) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1142.203 CPU sec, 66.336 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 661304, 46, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (126, 666) [M::mem_pestat] mean and std.dev: (397.22, 82.68) [M::mem_pestat] low and high boundaries for proper pairs: (18, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 33, 133) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 375) [M::mem_pestat] mean and std.dev: (39.51, 52.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 496) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1137.155 CPU sec, 50.384 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 661840, 49, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (312, 317, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (156, 546) [M::mem_pestat] mean and std.dev: (322.88, 34.07) [M::mem_pestat] low and high boundaries for proper pairs: (78, 624) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.20, 82.78) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 44, 1439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4273) [M::mem_pestat] mean and std.dev: (590.85, 1033.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5690) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1138.757 CPU sec, 65.920 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 662382, 51, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (211, 265, 1131) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2971) [M::mem_pestat] mean and std.dev: (596.30, 569.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3891) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.05, 82.84) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 25, 47) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 117) [M::mem_pestat] mean and std.dev: (28.36, 25.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 152) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1133.754 CPU sec, 50.171 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 662003, 44, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.09, 82.81) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 76, 694) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2050) [M::mem_pestat] mean and std.dev: (271.68, 479.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2728) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1141.869 CPU sec, 67.226 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 662295, 72, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.11, 82.68) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 38, 845) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2495) [M::mem_pestat] mean and std.dev: (216.65, 494.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3320) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1135.382 CPU sec, 50.442 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 662102, 51, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (255, 315, 333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (99, 489) [M::mem_pestat] mean and std.dev: (291.17, 68.85) [M::mem_pestat] low and high boundaries for proper pairs: (16, 567) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.08, 82.87) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 27, 130) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 360) [M::mem_pestat] mean and std.dev: (37.98, 44.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 475) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1143.858 CPU sec, 66.558 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 661869, 42, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.11, 82.78) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 26, 78) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 206) [M::mem_pestat] mean and std.dev: (28.00, 27.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 270) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1148.850 CPU sec, 67.606 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 661483, 52, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 303, 974) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2788) [M::mem_pestat] mean and std.dev: (446.50, 513.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3695) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.20, 82.72) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 48, 1075) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3181) [M::mem_pestat] mean and std.dev: (348.02, 714.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4234) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1136.667 CPU sec, 50.228 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 662600, 60, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.33, 82.71) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 38, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 578) [M::mem_pestat] mean and std.dev: (52.60, 80.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 762) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1145.430 CPU sec, 66.170 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 661574, 55, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (210, 354, 1069) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2787) [M::mem_pestat] mean and std.dev: (486.82, 460.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3646) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.13, 82.87) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 45, 133) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 363) [M::mem_pestat] mean and std.dev: (42.95, 51.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 478) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1143.729 CPU sec, 50.766 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 662218, 70, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.05, 82.77) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 29, 55) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 141) [M::mem_pestat] mean and std.dev: (27.75, 24.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 184) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1141.792 CPU sec, 65.371 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 661938, 44, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (256, 269, 915) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2233) [M::mem_pestat] mean and std.dev: (618.18, 533.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2892) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.31, 82.81) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 49, 431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1255) [M::mem_pestat] mean and std.dev: (124.41, 204.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1667) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1141.036 CPU sec, 50.626 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 662008, 53, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.35, 82.75) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 35, 81) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 215) [M::mem_pestat] mean and std.dev: (31.40, 27.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 282) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1138.624 CPU sec, 66.015 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 661866, 44, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (396.92, 82.70) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (8, 37, 74) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 206) [M::mem_pestat] mean and std.dev: (25.77, 22.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 272) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1132.055 CPU sec, 50.108 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 661974, 51, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.33, 82.83) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 41, 729) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2137) [M::mem_pestat] mean and std.dev: (189.77, 428.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2841) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1149.109 CPU sec, 68.330 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 661903, 47, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (262, 324, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (68, 553) [M::mem_pestat] mean and std.dev: (268.75, 79.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 650) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.22, 82.78) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 45, 956) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2820) [M::mem_pestat] mean and std.dev: (391.98, 723.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3752) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1136.234 CPU sec, 50.310 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 662127, 48, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 329, 686) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1880) [M::mem_pestat] mean and std.dev: (336.17, 253.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2477) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.15, 82.69) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 33, 84) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 214) [M::mem_pestat] mean and std.dev: (33.38, 25.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 279) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1124.774 CPU sec, 61.789 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 661827, 57, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (396.99, 82.84) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 35, 702) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2066) [M::mem_pestat] mean and std.dev: (162.43, 373.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2748) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1123.844 CPU sec, 49.664 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 662137, 50, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.12, 82.62) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 28, 87) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 231) [M::mem_pestat] mean and std.dev: (35.52, 40.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 303) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1130.998 CPU sec, 65.534 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 661874, 58, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.24, 82.76) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 32, 106) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 288) [M::mem_pestat] mean and std.dev: (36.61, 34.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 379) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1135.187 CPU sec, 50.227 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 661971, 58, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (274, 309, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 961) [M::mem_pestat] mean and std.dev: (359.00, 159.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1190) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.14, 82.85) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 40, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (37.68, 40.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 787) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1142.823 CPU sec, 66.662 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 662032, 56, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (237, 289, 466) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924) [M::mem_pestat] mean and std.dev: (279.40, 174.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1153) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (121, 671) [M::mem_pestat] mean and std.dev: (397.36, 82.96) [M::mem_pestat] low and high boundaries for proper pairs: (11, 781) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 47, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 786) [M::mem_pestat] mean and std.dev: (85.96, 151.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1040) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1137.607 CPU sec, 50.161 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 662464, 50, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.04, 82.77) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 53, 3126) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9330) [M::mem_pestat] mean and std.dev: (1529.74, 2343.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12432) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1139.481 CPU sec, 66.396 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 662651, 51, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.00, 82.73) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 32, 85) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 227) [M::mem_pestat] mean and std.dev: (31.12, 27.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 298) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1130.485 CPU sec, 50.071 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 662221, 49, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (126, 666) [M::mem_pestat] mean and std.dev: (397.17, 82.56) [M::mem_pestat] low and high boundaries for proper pairs: (18, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 28, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1245) [M::mem_pestat] mean and std.dev: (101.72, 264.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1654) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1134.907 CPU sec, 66.665 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 661955, 64, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (293, 421, 935) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2219) [M::mem_pestat] mean and std.dev: (705.50, 616.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3170) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.13, 82.72) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 38, 662) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1956) [M::mem_pestat] mean and std.dev: (77.22, 185.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2603) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1130.745 CPU sec, 50.066 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 661470, 55, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (269, 340, 566) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1160) [M::mem_pestat] mean and std.dev: (372.20, 188.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1457) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.10, 82.70) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 37, 77) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 191) [M::mem_pestat] mean and std.dev: (36.57, 32.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 248) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1146.016 CPU sec, 65.976 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 662006, 56, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.09, 82.78) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 31, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 740) [M::mem_pestat] mean and std.dev: (64.15, 94.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 980) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1137.102 CPU sec, 50.401 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 661676, 64, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (140, 403, 912) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2456) [M::mem_pestat] mean and std.dev: (438.91, 365.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3228) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.13, 82.75) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 31, 86) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 230) [M::mem_pestat] mean and std.dev: (38.40, 39.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 302) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1143.296 CPU sec, 65.516 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 661738, 49, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.17, 82.78) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (10, 32, 1524) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4552) [M::mem_pestat] mean and std.dev: (533.62, 1055.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6066) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1137.925 CPU sec, 50.475 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 661657, 52, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (231, 521, 1127) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2919) [M::mem_pestat] mean and std.dev: (544.10, 398.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3815) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (121, 671) [M::mem_pestat] mean and std.dev: (397.31, 82.84) [M::mem_pestat] low and high boundaries for proper pairs: (11, 781) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 32, 59) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 151) [M::mem_pestat] mean and std.dev: (32.39, 30.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 197) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1137.036 CPU sec, 64.776 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 662174, 53, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (717, 986, 2546) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6204) [M::mem_pestat] mean and std.dev: (1507.00, 1420.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8033) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (126, 666) [M::mem_pestat] mean and std.dev: (397.23, 82.56) [M::mem_pestat] low and high boundaries for proper pairs: (18, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 22, 66) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 174) [M::mem_pestat] mean and std.dev: (24.69, 24.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 228) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1135.207 CPU sec, 50.343 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 662627, 54, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.07, 82.81) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 60, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1141) [M::mem_pestat] mean and std.dev: (122.85, 173.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1515) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1138.771 CPU sec, 67.496 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 662179, 59, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.16, 82.71) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 44, 412) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1198) [M::mem_pestat] mean and std.dev: (119.35, 252.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1591) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1135.796 CPU sec, 50.277 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 662123, 51, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.15, 82.72) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 39, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670) [M::mem_pestat] mean and std.dev: (66.07, 107.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 886) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1143.910 CPU sec, 66.333 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 662006, 59, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (126, 666) [M::mem_pestat] mean and std.dev: (397.18, 82.60) [M::mem_pestat] low and high boundaries for proper pairs: (18, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 37, 77) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 193) [M::mem_pestat] mean and std.dev: (36.00, 31.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 251) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1144.071 CPU sec, 65.752 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 662461, 46, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (301, 318, 1108) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2722) [M::mem_pestat] mean and std.dev: (469.70, 389.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3529) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (121, 671) [M::mem_pestat] mean and std.dev: (397.29, 82.79) [M::mem_pestat] low and high boundaries for proper pairs: (11, 781) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 47, 697) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2041) [M::mem_pestat] mean and std.dev: (254.15, 475.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2713) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1136.541 CPU sec, 50.073 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 662325, 49, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (128, 415, 1099) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3041) [M::mem_pestat] mean and std.dev: (364.30, 325.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4012) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.12, 82.75) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 37, 459) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1343) [M::mem_pestat] mean and std.dev: (114.44, 196.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1785) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1147.255 CPU sec, 66.262 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 661678, 62, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (283, 419, 654) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1396) [M::mem_pestat] mean and std.dev: (406.55, 242.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1767) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.16, 82.61) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 44, 151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (54.55, 70.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 550) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1135.231 CPU sec, 50.348 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 662631, 63, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (366, 448, 1310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3198) [M::mem_pestat] mean and std.dev: (495.67, 333.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4142) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.02, 82.69) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 41, 115) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 301) [M::mem_pestat] mean and std.dev: (40.12, 32.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 394) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1139.166 CPU sec, 66.513 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 662468, 44, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.08, 82.65) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 35, 1262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3754) [M::mem_pestat] mean and std.dev: (363.63, 818.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5000) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1133.382 CPU sec, 50.128 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 661901, 53, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.36, 82.82) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 45, 1531) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4559) [M::mem_pestat] mean and std.dev: (753.25, 1318.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6073) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1143.244 CPU sec, 66.961 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 661751, 64, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.05, 82.72) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 26, 107) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 295) [M::mem_pestat] mean and std.dev: (31.65, 36.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 389) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1142.460 CPU sec, 50.606 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 661569, 53, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (340, 902, 2377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6451) [M::mem_pestat] mean and std.dev: (1454.30, 1716.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8488) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.15, 82.75) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 38, 150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (45.77, 44.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 537) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1140.190 CPU sec, 65.577 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 662254, 43, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (225, 313, 2340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6570) [M::mem_pestat] mean and std.dev: (979.00, 1105.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8685) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.11, 82.69) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 70, 2228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6644) [M::mem_pestat] mean and std.dev: (932.02, 1562.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8852) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1130.320 CPU sec, 50.172 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 661709, 65, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (277, 424, 2160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5926) [M::mem_pestat] mean and std.dev: (1015.73, 1263.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7809) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.12, 82.75) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 45, 1394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4142) [M::mem_pestat] mean and std.dev: (508.00, 991.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5516) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1148.018 CPU sec, 67.059 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 662370, 42, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.03, 82.73) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 39, 1608) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4792) [M::mem_pestat] mean and std.dev: (624.97, 1191.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6384) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1141.123 CPU sec, 50.485 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 661713, 51, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.08, 82.78) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 28, 124) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 344) [M::mem_pestat] mean and std.dev: (37.21, 38.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 454) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1148.433 CPU sec, 66.298 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 661566, 53, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (311, 461, 1147) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2819) [M::mem_pestat] mean and std.dev: (480.38, 317.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3655) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.14, 82.77) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 49, 1056) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3116) [M::mem_pestat] mean and std.dev: (331.65, 687.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4146) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1138.755 CPU sec, 50.433 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 661741, 47, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (126, 666) [M::mem_pestat] mean and std.dev: (397.08, 82.57) [M::mem_pestat] low and high boundaries for proper pairs: (18, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 38, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 790) [M::mem_pestat] mean and std.dev: (53.27, 86.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1047) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1149.849 CPU sec, 67.277 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 662088, 45, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (250, 305, 814) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1942) [M::mem_pestat] mean and std.dev: (497.92, 465.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2506) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.13, 82.77) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 26, 1933) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5763) [M::mem_pestat] mean and std.dev: (637.88, 1124.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7678) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1136.771 CPU sec, 50.259 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 662193, 51, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.02, 82.82) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 40, 152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424) [M::mem_pestat] mean and std.dev: (45.07, 48.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 560) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1138.072 CPU sec, 66.101 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 662395, 60, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (253, 298, 406) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 712) [M::mem_pestat] mean and std.dev: (283.42, 87.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 865) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.10, 82.81) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 44, 97) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 257) [M::mem_pestat] mean and std.dev: (47.90, 43.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1130.635 CPU sec, 50.061 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 662264, 58, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.09, 82.69) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 33, 433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1267) [M::mem_pestat] mean and std.dev: (94.74, 248.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1684) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1140.598 CPU sec, 65.981 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 662003, 62, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (126, 666) [M::mem_pestat] mean and std.dev: (397.26, 82.57) [M::mem_pestat] low and high boundaries for proper pairs: (18, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 59, 1241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3663) [M::mem_pestat] mean and std.dev: (590.09, 1044.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4874) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1137.235 CPU sec, 50.347 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 661617, 60, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (259, 844, 960) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2362) [M::mem_pestat] mean and std.dev: (655.40, 448.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3063) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.03, 82.54) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 48, 1704) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5078) [M::mem_pestat] mean and std.dev: (728.86, 1381.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6765) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1143.841 CPU sec, 66.342 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 661408, 55, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.04, 82.91) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 46, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454) [M::mem_pestat] mean and std.dev: (49.00, 52.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1139.959 CPU sec, 50.487 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 662323, 43, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (332, 397, 3625) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10211) [M::mem_pestat] mean and std.dev: (1756.73, 2378.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13504) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (396.95, 82.75) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 36, 1109) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3295) [M::mem_pestat] mean and std.dev: (337.53, 671.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4388) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1142.467 CPU sec, 66.496 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 661933, 67, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (218, 322, 566) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1262) [M::mem_pestat] mean and std.dev: (356.00, 307.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1610) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.17, 82.75) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 43, 132) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 344) [M::mem_pestat] mean and std.dev: (45.13, 42.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 450) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1137.382 CPU sec, 50.276 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 661771, 67, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.16, 82.69) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 45, 510) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1476) [M::mem_pestat] mean and std.dev: (119.24, 246.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1959) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1136.664 CPU sec, 64.820 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 662320, 63, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.26, 82.63) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 33, 1215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3613) [M::mem_pestat] mean and std.dev: (576.86, 1058.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4812) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1131.631 CPU sec, 50.049 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 661988, 52, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.37, 82.78) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 33, 61) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 135) [M::mem_pestat] mean and std.dev: (33.12, 19.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 172) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1139.264 CPU sec, 65.614 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 662247, 66, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.18, 82.87) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 35, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 849) [M::mem_pestat] mean and std.dev: (66.28, 122.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1126) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1131.339 CPU sec, 50.202 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 661816, 51, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.11, 82.66) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 37, 1068) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3168) [M::mem_pestat] mean and std.dev: (219.02, 593.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4218) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1132.870 CPU sec, 64.606 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 662024, 47, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (312, 521, 969) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2283) [M::mem_pestat] mean and std.dev: (548.43, 315.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2940) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (126, 666) [M::mem_pestat] mean and std.dev: (397.27, 82.63) [M::mem_pestat] low and high boundaries for proper pairs: (18, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 26, 44) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 106) [M::mem_pestat] mean and std.dev: (23.90, 17.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 137) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1147.440 CPU sec, 66.916 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 661954, 51, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (198, 389, 964) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2496) [M::mem_pestat] mean and std.dev: (452.55, 327.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3262) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.18, 82.87) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 57, 1616) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4794) [M::mem_pestat] mean and std.dev: (648.50, 1181.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6383) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1131.728 CPU sec, 50.040 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 662117, 47, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.14, 82.81) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 28, 79) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 201) [M::mem_pestat] mean and std.dev: (37.46, 34.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 262) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1141.905 CPU sec, 64.916 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 661847, 62, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (305, 368, 1026) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2468) [M::mem_pestat] mean and std.dev: (541.00, 438.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3189) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.05, 82.66) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 39, 133) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 357) [M::mem_pestat] mean and std.dev: (41.89, 36.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 469) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1128.377 CPU sec, 49.898 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 661827, 54, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.13, 82.75) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 38, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 514) [M::mem_pestat] mean and std.dev: (41.29, 40.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 677) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1133.887 CPU sec, 67.006 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 662351, 49, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.19, 82.79) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 37, 1095) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3251) [M::mem_pestat] mean and std.dev: (392.25, 844.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4329) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1134.142 CPU sec, 50.276 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 662145, 65, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (262, 427, 821) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1939) [M::mem_pestat] mean and std.dev: (480.82, 422.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2498) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (121, 671) [M::mem_pestat] mean and std.dev: (397.25, 83.00) [M::mem_pestat] low and high boundaries for proper pairs: (11, 781) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 39, 1199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3561) [M::mem_pestat] mean and std.dev: (369.70, 775.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4742) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1134.036 CPU sec, 65.467 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 662002, 56, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (178, 334, 1057) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2815) [M::mem_pestat] mean and std.dev: (463.17, 536.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3694) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (126, 666) [M::mem_pestat] mean and std.dev: (397.23, 82.67) [M::mem_pestat] low and high boundaries for proper pairs: (18, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 46, 1646) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4902) [M::mem_pestat] mean and std.dev: (735.23, 1247.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6530) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1133.071 CPU sec, 50.189 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 661520, 49, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.19, 82.88) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 39, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 718) [M::mem_pestat] mean and std.dev: (49.08, 57.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 951) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1135.094 CPU sec, 65.545 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 662112, 46, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.15, 82.77) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 35, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 816) [M::mem_pestat] mean and std.dev: (89.05, 176.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1080) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1126.193 CPU sec, 49.761 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 662319, 56, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.18, 82.70) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 36, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1084) [M::mem_pestat] mean and std.dev: (92.80, 201.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1440) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1130.382 CPU sec, 65.517 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 662396, 46, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.14, 82.78) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 30, 51) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 129) [M::mem_pestat] mean and std.dev: (27.85, 21.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 168) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1133.273 CPU sec, 50.196 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 661432, 55, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.03, 82.81) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 47, 119) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 319) [M::mem_pestat] mean and std.dev: (42.25, 36.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 419) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1135.221 CPU sec, 64.955 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 661745, 60, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (121, 671) [M::mem_pestat] mean and std.dev: (397.22, 82.90) [M::mem_pestat] low and high boundaries for proper pairs: (11, 781) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 44, 1350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4004) [M::mem_pestat] mean and std.dev: (474.76, 905.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5331) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1137.065 CPU sec, 49.943 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 662430, 64, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (269, 334, 1144) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2894) [M::mem_pestat] mean and std.dev: (651.10, 659.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3769) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.20, 82.78) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 45, 1009) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2987) [M::mem_pestat] mean and std.dev: (277.41, 571.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3976) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1140.737 CPU sec, 65.313 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 662574, 39, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.17, 82.75) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 40, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 451) [M::mem_pestat] mean and std.dev: (54.03, 57.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1124.214 CPU sec, 49.677 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 662079, 56, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.15, 82.71) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 34, 1141) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3391) [M::mem_pestat] mean and std.dev: (329.56, 726.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4516) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1131.777 CPU sec, 65.772 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 662773, 56, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (396.95, 82.91) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (11, 31, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (41.30, 67.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 679) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1121.965 CPU sec, 49.683 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 661228, 50, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (121, 671) [M::mem_pestat] mean and std.dev: (397.21, 82.93) [M::mem_pestat] low and high boundaries for proper pairs: (11, 781) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 50, 506) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1482) [M::mem_pestat] mean and std.dev: (71.87, 109.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1970) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1138.789 CPU sec, 67.141 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 661537, 46, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (278, 531, 1132) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2840) [M::mem_pestat] mean and std.dev: (660.82, 677.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3694) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (126, 666) [M::mem_pestat] mean and std.dev: (397.10, 82.52) [M::mem_pestat] low and high boundaries for proper pairs: (18, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 53, 685) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2003) [M::mem_pestat] mean and std.dev: (262.29, 466.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2662) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1132.447 CPU sec, 50.094 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 662172, 48, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (171, 318, 893) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2337) [M::mem_pestat] mean and std.dev: (426.75, 336.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3059) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (126, 666) [M::mem_pestat] mean and std.dev: (397.19, 82.59) [M::mem_pestat] low and high boundaries for proper pairs: (18, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 41, 1113) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3295) [M::mem_pestat] mean and std.dev: (333.71, 785.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4386) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1140.476 CPU sec, 66.051 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 662324, 54, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 228, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857) [M::mem_pestat] mean and std.dev: (173.78, 114.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1124) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (126, 666) [M::mem_pestat] mean and std.dev: (397.19, 82.60) [M::mem_pestat] low and high boundaries for proper pairs: (18, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 50, 1185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3521) [M::mem_pestat] mean and std.dev: (291.67, 666.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4689) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1127.575 CPU sec, 49.862 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 662469, 53, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (180, 340, 1123) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3009) [M::mem_pestat] mean and std.dev: (600.50, 573.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3952) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.19, 82.72) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 39, 158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 442) [M::mem_pestat] mean and std.dev: (39.43, 48.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 584) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1128.587 CPU sec, 65.024 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 661781, 48, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (238, 279, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (10, 580) [M::mem_pestat] mean and std.dev: (256.55, 118.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 729) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.18, 82.62) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 34, 733) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2167) [M::mem_pestat] mean and std.dev: (191.22, 440.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2884) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1131.121 CPU sec, 50.095 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 661566, 64, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (245, 315, 403) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719) [M::mem_pestat] mean and std.dev: (269.00, 122.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 877) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (121, 671) [M::mem_pestat] mean and std.dev: (397.22, 82.86) [M::mem_pestat] low and high boundaries for proper pairs: (11, 781) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 53, 895) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2643) [M::mem_pestat] mean and std.dev: (276.30, 491.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3517) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1126.715 CPU sec, 64.731 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 661749, 62, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (121, 671) [M::mem_pestat] mean and std.dev: (397.35, 82.94) [M::mem_pestat] low and high boundaries for proper pairs: (11, 781) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 45, 68) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 166) [M::mem_pestat] mean and std.dev: (31.47, 19.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 215) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1130.327 CPU sec, 49.970 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 661888, 67, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (198, 299, 420) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864) [M::mem_pestat] mean and std.dev: (288.50, 148.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1086) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (121, 671) [M::mem_pestat] mean and std.dev: (397.26, 82.87) [M::mem_pestat] low and high boundaries for proper pairs: (11, 781) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 53, 2590) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7724) [M::mem_pestat] mean and std.dev: (913.30, 1684.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10291) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1130.305 CPU sec, 65.026 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 662455, 50, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (299, 415, 2771) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7715) [M::mem_pestat] mean and std.dev: (764.91, 790.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10187) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (121, 671) [M::mem_pestat] mean and std.dev: (397.31, 82.99) [M::mem_pestat] low and high boundaries for proper pairs: (11, 781) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 40, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 626) [M::mem_pestat] mean and std.dev: (86.52, 145.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 829) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1121.359 CPU sec, 49.639 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 662630, 53, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.04, 82.72) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 40, 94) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 248) [M::mem_pestat] mean and std.dev: (36.23, 28.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 325) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1129.978 CPU sec, 64.768 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 661744, 43, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.15, 82.76) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 37, 1100) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3262) [M::mem_pestat] mean and std.dev: (480.95, 868.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4343) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1142.370 CPU sec, 66.567 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 661724, 57, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (132, 272, 825) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2211) [M::mem_pestat] mean and std.dev: (364.00, 316.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2904) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.11, 82.86) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 55, 383) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1103) [M::mem_pestat] mean and std.dev: (110.79, 186.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1463) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1131.696 CPU sec, 50.049 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 662147, 60, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.46, 82.78) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 44, 126) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 334) [M::mem_pestat] mean and std.dev: (48.73, 49.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 438) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1130.320 CPU sec, 64.673 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 662232, 53, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (126, 666) [M::mem_pestat] mean and std.dev: (397.07, 82.69) [M::mem_pestat] low and high boundaries for proper pairs: (18, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 29, 120) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 330) [M::mem_pestat] mean and std.dev: (43.87, 51.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 435) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1123.288 CPU sec, 49.743 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 661957, 42, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.01, 82.71) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 38, 562) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1650) [M::mem_pestat] mean and std.dev: (174.83, 360.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2194) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1127.190 CPU sec, 65.375 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 662217, 65, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.01, 82.85) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 39, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (42.88, 53.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 617) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1118.941 CPU sec, 49.421 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 661674, 44, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (305, 402, 779) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1727) [M::mem_pestat] mean and std.dev: (515.50, 376.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2201) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (126, 666) [M::mem_pestat] mean and std.dev: (397.24, 82.53) [M::mem_pestat] low and high boundaries for proper pairs: (18, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 41, 140) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 366) [M::mem_pestat] mean and std.dev: (51.94, 50.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 479) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1128.195 CPU sec, 64.164 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 662339, 53, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.17, 82.64) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 29, 98) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 266) [M::mem_pestat] mean and std.dev: (32.27, 32.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 350) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1125.320 CPU sec, 49.737 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 661802, 46, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (268, 372, 1145) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2899) [M::mem_pestat] mean and std.dev: (541.73, 524.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3776) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (121, 671) [M::mem_pestat] mean and std.dev: (397.40, 82.89) [M::mem_pestat] low and high boundaries for proper pairs: (11, 781) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (8, 25, 77) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 215) [M::mem_pestat] mean and std.dev: (44.88, 53.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 284) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1130.437 CPU sec, 64.576 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 662071, 54, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (314, 701, 1218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3026) [M::mem_pestat] mean and std.dev: (620.78, 368.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3930) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (121, 671) [M::mem_pestat] mean and std.dev: (397.29, 82.92) [M::mem_pestat] low and high boundaries for proper pairs: (11, 781) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 39, 116) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 318) [M::mem_pestat] mean and std.dev: (46.28, 53.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 419) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1125.821 CPU sec, 49.818 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 661360, 60, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (351, 939, 1637) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4209) [M::mem_pestat] mean and std.dev: (847.07, 637.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5495) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (121, 671) [M::mem_pestat] mean and std.dev: (397.13, 82.99) [M::mem_pestat] low and high boundaries for proper pairs: (11, 781) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 48, 498) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1448) [M::mem_pestat] mean and std.dev: (118.52, 231.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1923) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1129.437 CPU sec, 64.683 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 661363, 44, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.15, 82.92) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (10, 31, 108) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 304) [M::mem_pestat] mean and std.dev: (36.43, 41.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 402) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1125.259 CPU sec, 49.757 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 662708, 61, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (126, 666) [M::mem_pestat] mean and std.dev: (397.20, 82.60) [M::mem_pestat] low and high boundaries for proper pairs: (18, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 43, 136) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 370) [M::mem_pestat] mean and std.dev: (50.12, 56.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 487) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1122.256 CPU sec, 65.180 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 661848, 45, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.17, 82.77) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 30, 544) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1596) [M::mem_pestat] mean and std.dev: (135.03, 310.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2122) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1123.668 CPU sec, 49.689 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 662219, 41, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (397, 653, 1493) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3685) [M::mem_pestat] mean and std.dev: (835.40, 710.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4781) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.04, 82.75) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 59, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 790) [M::mem_pestat] mean and std.dev: (73.55, 93.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1045) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1125.660 CPU sec, 63.453 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 661573, 58, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (126, 666) [M::mem_pestat] mean and std.dev: (397.21, 82.61) [M::mem_pestat] low and high boundaries for proper pairs: (18, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 44, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 538) [M::mem_pestat] mean and std.dev: (42.09, 45.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 713) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1122.871 CPU sec, 49.746 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 662079, 72, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (275, 452, 1086) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2708) [M::mem_pestat] mean and std.dev: (604.00, 510.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3519) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.08, 82.80) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 50, 1663) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4937) [M::mem_pestat] mean and std.dev: (809.34, 1422.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6574) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1125.080 CPU sec, 65.089 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 662152, 45, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.01, 82.78) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 61, 1099) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3265) [M::mem_pestat] mean and std.dev: (333.21, 686.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4348) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1124.670 CPU sec, 49.735 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 662548, 39, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (143, 209, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830) [M::mem_pestat] mean and std.dev: (261.94, 202.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1071) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.02, 82.62) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 33, 115) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 305) [M::mem_pestat] mean and std.dev: (43.76, 55.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 400) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1126.856 CPU sec, 65.166 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 661781, 54, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (256, 485, 2337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6499) [M::mem_pestat] mean and std.dev: (953.36, 1287.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8580) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.09, 82.80) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 41, 606) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1776) [M::mem_pestat] mean and std.dev: (120.25, 259.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2361) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1127.403 CPU sec, 49.944 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 661789, 47, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (396.99, 82.63) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 31, 60) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 148) [M::mem_pestat] mean and std.dev: (27.66, 22.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 192) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1133.568 CPU sec, 64.363 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 662269, 58, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.04, 82.76) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 41, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (43.64, 68.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1120.435 CPU sec, 49.581 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 662236, 57, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (126, 666) [M::mem_pestat] mean and std.dev: (397.19, 82.59) [M::mem_pestat] low and high boundaries for proper pairs: (18, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 34, 137) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 371) [M::mem_pestat] mean and std.dev: (47.79, 47.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 488) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1119.813 CPU sec, 61.125 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 661695, 70, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.12, 82.84) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 41, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479) [M::mem_pestat] mean and std.dev: (53.30, 61.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 633) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1125.964 CPU sec, 49.747 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 662171, 60, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 350, 388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 996) [M::mem_pestat] mean and std.dev: (269.64, 184.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1300) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (121, 671) [M::mem_pestat] mean and std.dev: (397.29, 82.80) [M::mem_pestat] low and high boundaries for proper pairs: (11, 781) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 36, 106) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 288) [M::mem_pestat] mean and std.dev: (35.20, 41.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 379) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1126.577 CPU sec, 64.779 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 662034, 48, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.13, 82.72) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 33, 59) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 151) [M::mem_pestat] mean and std.dev: (24.55, 18.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 197) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1129.780 CPU sec, 50.103 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 662257, 65, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.05, 82.60) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (10, 34, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (54.41, 79.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 646) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1139.876 CPU sec, 66.697 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 661972, 46, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (126, 666) [M::mem_pestat] mean and std.dev: (397.16, 82.69) [M::mem_pestat] low and high boundaries for proper pairs: (18, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 33, 100) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 272) [M::mem_pestat] mean and std.dev: (41.47, 45.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 358) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1138.621 CPU sec, 50.420 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 662054, 46, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (221, 289, 1210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3188) [M::mem_pestat] mean and std.dev: (540.36, 467.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4177) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.30, 82.65) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 33, 120) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 334) [M::mem_pestat] mean and std.dev: (37.30, 37.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 441) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1145.486 CPU sec, 64.206 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 662079, 60, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.07, 82.71) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 36, 65) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 155) [M::mem_pestat] mean and std.dev: (36.63, 32.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 200) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1138.804 CPU sec, 66.178 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 662084, 43, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (183, 205, 467) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1035) [M::mem_pestat] mean and std.dev: (227.85, 157.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1319) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.22, 82.75) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 39, 979) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2903) [M::mem_pestat] mean and std.dev: (199.86, 524.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3865) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1136.538 CPU sec, 50.382 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 661687, 56, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 327, 814) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2300) [M::mem_pestat] mean and std.dev: (451.80, 409.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3043) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.12, 82.83) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 34, 63) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 163) [M::mem_pestat] mean and std.dev: (25.95, 18.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 213) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1141.574 CPU sec, 65.288 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 662200, 47, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (396.96, 82.68) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 37, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1024) [M::mem_pestat] mean and std.dev: (61.42, 98.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1359) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1132.484 CPU sec, 50.035 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 661852, 56, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.19, 82.81) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 36, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609) [M::mem_pestat] mean and std.dev: (61.82, 112.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 807) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1125.123 CPU sec, 65.161 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 661909, 55, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.18, 82.84) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 44, 148) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400) [M::mem_pestat] mean and std.dev: (51.96, 56.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 526) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1120.623 CPU sec, 49.629 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 661682, 52, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (126, 666) [M::mem_pestat] mean and std.dev: (397.39, 82.66) [M::mem_pestat] low and high boundaries for proper pairs: (18, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 53, 551) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1615) [M::mem_pestat] mean and std.dev: (92.24, 163.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2147) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1128.158 CPU sec, 64.197 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 661818, 36, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.09, 82.66) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 51, 1377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4085) [M::mem_pestat] mean and std.dev: (334.45, 682.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5439) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1128.252 CPU sec, 49.891 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 662340, 59, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 252, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1162) [M::mem_pestat] mean and std.dev: (207.75, 124.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1520) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.21, 82.76) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 28, 102) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 272) [M::mem_pestat] mean and std.dev: (38.38, 39.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 357) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1119.665 CPU sec, 63.867 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 661799, 54, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (126, 666) [M::mem_pestat] mean and std.dev: (397.13, 82.55) [M::mem_pestat] low and high boundaries for proper pairs: (18, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 33, 108) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 292) [M::mem_pestat] mean and std.dev: (30.17, 30.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 384) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1123.316 CPU sec, 49.678 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 661259, 52, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (194, 332, 754) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1874) [M::mem_pestat] mean and std.dev: (355.30, 288.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2434) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.17, 82.80) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 38, 1688) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5028) [M::mem_pestat] mean and std.dev: (459.11, 1011.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6698) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1131.553 CPU sec, 64.882 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 661974, 60, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (126, 666) [M::mem_pestat] mean and std.dev: (397.17, 82.65) [M::mem_pestat] low and high boundaries for proper pairs: (18, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 41, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 771) [M::mem_pestat] mean and std.dev: (48.34, 66.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1024) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1124.063 CPU sec, 49.693 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 662209, 55, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.16, 82.63) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 33, 72) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 186) [M::mem_pestat] mean and std.dev: (34.70, 29.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 243) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1121.489 CPU sec, 63.651 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 662510, 50, 10) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.09, 82.69) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 56, 2599) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7751) [M::mem_pestat] mean and std.dev: (1126.89, 2010.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10327) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (376, 411, 1440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3568) [M::mem_pestat] mean and std.dev: (531.88, 419.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4632) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1124.753 CPU sec, 49.760 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 661597, 56, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (121, 671) [M::mem_pestat] mean and std.dev: (397.32, 82.91) [M::mem_pestat] low and high boundaries for proper pairs: (11, 781) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 36, 90) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 240) [M::mem_pestat] mean and std.dev: (34.11, 33.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 315) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1127.697 CPU sec, 65.055 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 661567, 43, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.07, 82.79) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 46, 1954) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5818) [M::mem_pestat] mean and std.dev: (694.59, 1196.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7750) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1126.285 CPU sec, 49.857 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 661698, 67, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (302, 443, 1108) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2720) [M::mem_pestat] mean and std.dev: (635.22, 527.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3526) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.17, 82.86) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 35, 145) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403) [M::mem_pestat] mean and std.dev: (55.50, 80.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 532) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1123.625 CPU sec, 63.994 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 662173, 54, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.21, 82.59) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 37, 144) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 392) [M::mem_pestat] mean and std.dev: (53.36, 69.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 516) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1122.868 CPU sec, 49.706 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 661780, 60, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (215, 273, 980) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2510) [M::mem_pestat] mean and std.dev: (441.15, 384.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3275) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (396.98, 82.80) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 32, 103) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 281) [M::mem_pestat] mean and std.dev: (37.98, 43.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 370) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1126.649 CPU sec, 63.574 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 662279, 55, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.23, 82.83) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 36, 551) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1611) [M::mem_pestat] mean and std.dev: (107.77, 285.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2141) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1129.100 CPU sec, 64.079 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 661902, 42, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (289, 361, 409) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (49, 649) [M::mem_pestat] mean and std.dev: (315.88, 64.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 769) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.17, 82.68) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 45, 661) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1943) [M::mem_pestat] mean and std.dev: (165.43, 295.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2584) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1131.326 CPU sec, 50.078 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 662268, 50, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (397, 601, 1312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3142) [M::mem_pestat] mean and std.dev: (552.22, 335.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4057) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.07, 82.68) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (9, 27, 54) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 144) [M::mem_pestat] mean and std.dev: (23.66, 19.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 189) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1125.428 CPU sec, 64.365 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 661990, 44, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (312, 320, 407) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 597) [M::mem_pestat] mean and std.dev: (335.00, 38.73) [M::mem_pestat] low and high boundaries for proper pairs: (27, 692) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (121, 671) [M::mem_pestat] mean and std.dev: (397.22, 82.88) [M::mem_pestat] low and high boundaries for proper pairs: (11, 781) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 32, 953) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2819) [M::mem_pestat] mean and std.dev: (286.45, 663.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3752) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1127.424 CPU sec, 49.803 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 662157, 52, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.12, 82.64) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 40, 86) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 228) [M::mem_pestat] mean and std.dev: (43.07, 44.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 299) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1127.701 CPU sec, 64.565 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 661750, 61, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (215, 302, 355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635) [M::mem_pestat] mean and std.dev: (279.40, 119.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 775) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 392, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.33, 82.79) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 32, 894) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2652) [M::mem_pestat] mean and std.dev: (178.16, 482.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3531) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1122.139 CPU sec, 49.625 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 662039, 68, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (126, 666) [M::mem_pestat] mean and std.dev: (397.20, 82.61) [M::mem_pestat] low and high boundaries for proper pairs: (18, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 38, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692) [M::mem_pestat] mean and std.dev: (69.70, 120.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 916) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1120.371 CPU sec, 64.589 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 662005, 43, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 283, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 781) [M::mem_pestat] mean and std.dev: (232.22, 202.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1042) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.02, 82.72) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 32, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567) [M::mem_pestat] mean and std.dev: (57.69, 89.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 750) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1126.327 CPU sec, 49.910 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 661843, 52, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.12, 82.64) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 43, 121) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 315) [M::mem_pestat] mean and std.dev: (38.00, 28.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 412) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1125.808 CPU sec, 64.423 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 661805, 53, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (213, 298, 496) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1062) [M::mem_pestat] mean and std.dev: (299.64, 159.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1345) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (126, 666) [M::mem_pestat] mean and std.dev: (397.19, 82.51) [M::mem_pestat] low and high boundaries for proper pairs: (18, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 28, 93) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 249) [M::mem_pestat] mean and std.dev: (27.68, 22.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 327) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1121.375 CPU sec, 49.491 real sec [M::process] read 971496 sequences (145724400 bp)... [M::process] 0 single-end sequences; 971496 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 402095, 30, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.02, 82.70) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 37, 79) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 205) [M::mem_pestat] mean and std.dev: (29.38, 23.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 268) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 971496 reads in 673.222 CPU sec, 29.131 real sec [main] Version: 0.7.17-r1188 [main] CMD: /home/csyk2proj/tools/bwa.kit/bwa mem -p -t24 -R@RG\tID:KPGP-00345\tPL:ILLUMINA\tSM:KPGP-00345 -C /home/csyk2proj/data/bwa.kit/hs38DH.fa - [main] Real time: 56625.892 sec; CPU: 690630.877 sec