[M::bwa_idx_load_from_disk] read 3171 ALT contigs [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (72, 666862, 24, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (35, 58, 156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 398) [M::mem_pestat] mean and std.dev: (88.54, 75.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 519) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.37, 75.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (41, 105, 587) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1679) [M::mem_pestat] mean and std.dev: (126.10, 182.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2225) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (773, 1899, 2285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5309) [M::mem_pestat] mean and std.dev: (1423.08, 742.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6821) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 935.263 CPU sec, 54.538 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (61, 667316, 34, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (40, 66, 148) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 364) [M::mem_pestat] mean and std.dev: (89.40, 70.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 472) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.53, 75.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 65, 137) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 355) [M::mem_pestat] mean and std.dev: (60.75, 50.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 464) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 925.166 CPU sec, 41.071 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (82, 666937, 37, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (40, 68, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403) [M::mem_pestat] mean and std.dev: (94.13, 72.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 524) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 262, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (11, 521) [M::mem_pestat] mean and std.dev: (269.13, 75.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (81, 123, 2331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6831) [M::mem_pestat] mean and std.dev: (974.06, 1491.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9081) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (595, 827, 1033) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1909) [M::mem_pestat] mean and std.dev: (637.00, 323.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2347) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 925.829 CPU sec, 52.536 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (69, 666345, 28, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (38, 69, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 557) [M::mem_pestat] mean and std.dev: (91.10, 74.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 730) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.39, 75.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 44, 848) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2498) [M::mem_pestat] mean and std.dev: (210.67, 396.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3323) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (607, 1027, 2730) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6976) [M::mem_pestat] mean and std.dev: (1693.80, 1669.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9099) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 922.947 CPU sec, 40.961 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (90, 666674, 24, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (37, 69, 142) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 352) [M::mem_pestat] mean and std.dev: (81.21, 58.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 457) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (11, 521) [M::mem_pestat] mean and std.dev: (269.12, 75.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 57, 1757) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5235) [M::mem_pestat] mean and std.dev: (955.12, 1453.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6974) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (679, 1076, 1749) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3889) [M::mem_pestat] mean and std.dev: (1111.58, 880.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4959) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 920.467 CPU sec, 53.038 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (93, 667499, 25, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (40, 65, 128) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 304) [M::mem_pestat] mean and std.dev: (76.26, 57.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 392) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.37, 75.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 83, 790) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2312) [M::mem_pestat] mean and std.dev: (334.04, 523.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3073) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 922.720 CPU sec, 40.813 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (82, 666407, 21, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (47, 73, 151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 359) [M::mem_pestat] mean and std.dev: (94.67, 71.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 463) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.37, 75.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 89, 4406) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13176) [M::mem_pestat] mean and std.dev: (1786.33, 2608.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17561) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (720, 1017, 1685) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3615) [M::mem_pestat] mean and std.dev: (1255.00, 975.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5157) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 931.701 CPU sec, 53.655 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (61, 666492, 25, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 87, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (110.98, 78.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 613) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.28, 75.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (82, 133, 396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1024) [M::mem_pestat] mean and std.dev: (114.60, 86.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1338) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 928.237 CPU sec, 41.195 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (70, 667178, 36, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (42, 95, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 453) [M::mem_pestat] mean and std.dev: (105.14, 80.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.34, 75.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (43, 111, 155) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 379) [M::mem_pestat] mean and std.dev: (88.16, 55.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 491) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 922.474 CPU sec, 53.624 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (74, 666531, 23, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (38, 73, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 485) [M::mem_pestat] mean and std.dev: (97.91, 96.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 634) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.33, 75.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (50, 159, 1654) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4862) [M::mem_pestat] mean and std.dev: (879.41, 1330.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6466) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (688, 1142, 1574) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3346) [M::mem_pestat] mean and std.dev: (1178.06, 759.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4232) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 930.371 CPU sec, 41.602 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (78, 666160, 26, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (37, 61, 152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 382) [M::mem_pestat] mean and std.dev: (89.93, 76.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 497) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.59, 75.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (51, 96, 951) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2751) [M::mem_pestat] mean and std.dev: (291.14, 559.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3651) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 930.842 CPU sec, 53.614 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (76, 667094, 40, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (34, 47, 136) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 340) [M::mem_pestat] mean and std.dev: (70.26, 59.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 442) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.35, 75.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 100, 2038) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6054) [M::mem_pestat] mean and std.dev: (763.57, 1284.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8062) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (642, 967, 1631) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3609) [M::mem_pestat] mean and std.dev: (1076.80, 648.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4598) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 923.541 CPU sec, 41.060 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (71, 666910, 30, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (44, 74, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 527) [M::mem_pestat] mean and std.dev: (94.98, 76.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 688) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.51, 75.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 67, 1126) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3316) [M::mem_pestat] mean and std.dev: (412.96, 787.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4411) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (467, 707, 1395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3251) [M::mem_pestat] mean and std.dev: (837.27, 557.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4179) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 926.663 CPU sec, 53.855 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (72, 666975, 26, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (35, 63, 123) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 299) [M::mem_pestat] mean and std.dev: (81.93, 61.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 387) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.46, 75.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 62, 1676) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4958) [M::mem_pestat] mean and std.dev: (635.83, 1181.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6599) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 913.711 CPU sec, 40.572 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (84, 666832, 24, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (41, 101, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 542) [M::mem_pestat] mean and std.dev: (101.48, 75.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 709) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.43, 75.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 116, 374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1052) [M::mem_pestat] mean and std.dev: (111.70, 131.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1391) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (683, 863, 1428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2918) [M::mem_pestat] mean and std.dev: (875.83, 438.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3663) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 929.069 CPU sec, 54.660 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (82, 666712, 32, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (36, 72, 157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 399) [M::mem_pestat] mean and std.dev: (91.11, 78.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 520) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.62, 75.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 55, 768) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2230) [M::mem_pestat] mean and std.dev: (220.29, 408.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2961) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (360, 684, 1880) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4920) [M::mem_pestat] mean and std.dev: (953.21, 710.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6440) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 923.365 CPU sec, 40.999 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (62, 666799, 37, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (39, 59, 118) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 276) [M::mem_pestat] mean and std.dev: (78.84, 60.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 355) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.30, 75.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 52, 916) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2708) [M::mem_pestat] mean and std.dev: (253.52, 638.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3604) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (772, 1079, 2325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5431) [M::mem_pestat] mean and std.dev: (1173.64, 620.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6984) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 927.163 CPU sec, 54.208 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (73, 666761, 33, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (41, 82, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 539) [M::mem_pestat] mean and std.dev: (97.28, 74.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 705) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.25, 75.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (44, 117, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1028) [M::mem_pestat] mean and std.dev: (141.61, 163.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1356) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (232, 622, 908) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2260) [M::mem_pestat] mean and std.dev: (730.57, 586.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3078) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 922.981 CPU sec, 41.022 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (67, 666928, 32, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (50, 75, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (95.74, 71.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 518) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.42, 75.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 101, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906) [M::mem_pestat] mean and std.dev: (82.12, 78.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1198) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (511, 752, 2919) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7735) [M::mem_pestat] mean and std.dev: (1259.92, 1246.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10143) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 916.524 CPU sec, 53.792 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (63, 666568, 21, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (44, 86, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (100.69, 87.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 604) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (216, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (12, 522) [M::mem_pestat] mean and std.dev: (269.47, 75.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 624) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 80, 1961) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5815) [M::mem_pestat] mean and std.dev: (662.05, 1351.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7742) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (450, 828, 1550) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3750) [M::mem_pestat] mean and std.dev: (956.31, 673.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4850) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 914.902 CPU sec, 40.655 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (75, 666571, 29, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (38, 67, 143) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 353) [M::mem_pestat] mean and std.dev: (78.64, 57.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 458) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.36, 75.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 79, 123) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 327) [M::mem_pestat] mean and std.dev: (63.17, 49.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 429) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (472, 808, 1837) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4567) [M::mem_pestat] mean and std.dev: (1260.73, 1166.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5932) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 910.559 CPU sec, 50.710 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (76, 667213, 25, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (43, 91, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400) [M::mem_pestat] mean and std.dev: (94.25, 69.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 519) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.40, 75.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 130, 1610) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4768) [M::mem_pestat] mean and std.dev: (752.54, 1112.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6347) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (607, 1044, 1814) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4228) [M::mem_pestat] mean and std.dev: (1166.13, 987.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5435) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 906.409 CPU sec, 40.105 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (88, 666603, 35, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (36, 69, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 453) [M::mem_pestat] mean and std.dev: (83.90, 64.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 592) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.41, 75.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 66, 145) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 377) [M::mem_pestat] mean and std.dev: (73.10, 68.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 493) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (661, 849, 1484) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3130) [M::mem_pestat] mean and std.dev: (868.40, 570.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3953) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 909.150 CPU sec, 51.386 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (75, 666665, 33, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (41, 68, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 413) [M::mem_pestat] mean and std.dev: (96.51, 87.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 537) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.43, 75.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (52, 89, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454) [M::mem_pestat] mean and std.dev: (87.81, 52.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 588) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (689, 1016, 2442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5948) [M::mem_pestat] mean and std.dev: (1347.73, 1219.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7701) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 907.243 CPU sec, 40.343 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (73, 666676, 19, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (47, 71, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 389) [M::mem_pestat] mean and std.dev: (86.37, 68.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 503) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.28, 75.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (49, 95, 137) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 313) [M::mem_pestat] mean and std.dev: (78.06, 43.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 401) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (280, 821, 1450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3790) [M::mem_pestat] mean and std.dev: (805.22, 653.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4960) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 909.249 CPU sec, 49.911 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (77, 666601, 30, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (38, 85, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488) [M::mem_pestat] mean and std.dev: (100.20, 89.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 638) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.48, 75.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (47, 96, 159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 383) [M::mem_pestat] mean and std.dev: (75.96, 47.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 495) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1012, 1733, 2801) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6379) [M::mem_pestat] mean and std.dev: (1864.67, 1503.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8168) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 900.970 CPU sec, 39.821 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (77, 667117, 36, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (37, 77, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (86.06, 66.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 549) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.42, 75.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 65, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682) [M::mem_pestat] mean and std.dev: (74.72, 69.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 898) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (449, 969, 1135) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2507) [M::mem_pestat] mean and std.dev: (753.89, 419.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3193) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 906.746 CPU sec, 49.546 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (66, 667095, 31, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (45, 80, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (116.68, 108.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 609) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.51, 75.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 104, 594) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1730) [M::mem_pestat] mean and std.dev: (148.48, 319.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2298) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (259, 979, 2251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6235) [M::mem_pestat] mean and std.dev: (1162.67, 1175.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8227) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 904.121 CPU sec, 39.960 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (68, 666842, 26, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (42, 69, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 453) [M::mem_pestat] mean and std.dev: (83.02, 67.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.43, 75.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 66, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925) [M::mem_pestat] mean and std.dev: (125.09, 207.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1223) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (463, 860, 1639) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3991) [M::mem_pestat] mean and std.dev: (1074.73, 778.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5167) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 909.075 CPU sec, 50.825 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (85, 666685, 29, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (41, 68, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 428) [M::mem_pestat] mean and std.dev: (94.09, 71.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 557) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.36, 75.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 73, 526) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1506) [M::mem_pestat] mean and std.dev: (136.38, 255.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1996) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (286, 935, 1242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3154) [M::mem_pestat] mean and std.dev: (861.50, 631.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4110) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 927.748 CPU sec, 54.052 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (85, 666749, 35, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (35, 55, 126) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 308) [M::mem_pestat] mean and std.dev: (79.28, 63.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 399) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.56, 75.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 104, 2198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6522) [M::mem_pestat] mean and std.dev: (601.52, 1190.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8684) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (584, 740, 1227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2513) [M::mem_pestat] mean and std.dev: (875.91, 611.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3321) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 917.997 CPU sec, 40.907 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (74, 667157, 28, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (37, 53, 125) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 301) [M::mem_pestat] mean and std.dev: (80.74, 68.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 389) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.45, 75.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (57, 126, 1257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3657) [M::mem_pestat] mean and std.dev: (454.84, 838.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4857) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (716, 933, 1732) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3764) [M::mem_pestat] mean and std.dev: (1065.67, 640.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4780) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 924.968 CPU sec, 54.335 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (64, 667117, 35, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (40, 71, 380) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1060) [M::mem_pestat] mean and std.dev: (177.29, 228.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1400) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.41, 75.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 73, 136) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 344) [M::mem_pestat] mean and std.dev: (59.50, 41.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 448) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (418, 541, 1011) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2197) [M::mem_pestat] mean and std.dev: (654.07, 471.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2790) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 919.733 CPU sec, 40.950 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (81, 666912, 28, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (44, 72, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 398) [M::mem_pestat] mean and std.dev: (91.87, 71.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 516) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.51, 75.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 64, 2268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6736) [M::mem_pestat] mean and std.dev: (762.19, 1418.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8970) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (649, 1059, 3114) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8044) [M::mem_pestat] mean and std.dev: (1326.09, 1198.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10509) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 918.108 CPU sec, 53.708 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (89, 666690, 17, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (40, 82, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 565) [M::mem_pestat] mean and std.dev: (97.84, 100.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 740) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.29, 75.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 44, 138) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 372) [M::mem_pestat] mean and std.dev: (52.07, 45.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 489) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (811, 962, 2278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5212) [M::mem_pestat] mean and std.dev: (1059.08, 768.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6679) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 910.376 CPU sec, 40.519 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (73, 666513, 27, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (44, 74, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (97.58, 86.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 640) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.33, 75.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 121, 3236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9662) [M::mem_pestat] mean and std.dev: (1434.52, 1864.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12875) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (909, 1339, 3417) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8433) [M::mem_pestat] mean and std.dev: (1501.18, 1106.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10941) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 909.308 CPU sec, 51.256 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (73, 666673, 23, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (44, 86, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 575) [M::mem_pestat] mean and std.dev: (101.10, 87.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 752) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.49, 75.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (52, 80, 1763) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5185) [M::mem_pestat] mean and std.dev: (774.45, 1023.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6896) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (750, 990, 1556) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3168) [M::mem_pestat] mean and std.dev: (1098.93, 569.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3974) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 900.739 CPU sec, 39.836 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (86, 666629, 24, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (39, 66, 154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 384) [M::mem_pestat] mean and std.dev: (87.64, 69.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 499) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.36, 75.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 69, 1741) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5153) [M::mem_pestat] mean and std.dev: (828.70, 1552.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7038) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 901.040 CPU sec, 50.308 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (63, 667620, 26, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (40, 61, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463) [M::mem_pestat] mean and std.dev: (82.89, 77.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 604) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.42, 75.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (72, 163, 1572) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4572) [M::mem_pestat] mean and std.dev: (769.28, 1010.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6072) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (722, 1114, 1582) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3302) [M::mem_pestat] mean and std.dev: (1072.31, 525.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4162) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 902.694 CPU sec, 40.031 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (79, 666690, 32, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (37, 52, 158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400) [M::mem_pestat] mean and std.dev: (74.38, 69.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 521) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.31, 75.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 76, 120) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 288) [M::mem_pestat] mean and std.dev: (59.81, 37.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 372) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (857, 1067, 1926) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4064) [M::mem_pestat] mean and std.dev: (1231.23, 570.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5133) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 902.277 CPU sec, 49.691 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (77, 667004, 31, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (44, 69, 134) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 314) [M::mem_pestat] mean and std.dev: (79.65, 55.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 404) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.37, 75.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 63, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 728) [M::mem_pestat] mean and std.dev: (73.00, 71.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 961) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 896.747 CPU sec, 39.651 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (76, 666586, 26, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (36, 72, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 522) [M::mem_pestat] mean and std.dev: (92.48, 88.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 684) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.47, 75.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 133, 1632) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4858) [M::mem_pestat] mean and std.dev: (754.58, 1391.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6471) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (189, 976, 1351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3675) [M::mem_pestat] mean and std.dev: (1039.57, 873.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4837) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 907.291 CPU sec, 50.894 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (90, 666830, 18, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (33, 62, 154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 396) [M::mem_pestat] mean and std.dev: (83.17, 70.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 517) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.49, 75.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 89, 2223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6615) [M::mem_pestat] mean and std.dev: (1124.35, 1692.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8811) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (796, 906, 1957) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4279) [M::mem_pestat] mean and std.dev: (996.70, 728.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5440) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 903.401 CPU sec, 40.026 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (89, 667716, 28, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (38, 74, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (92.65, 75.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 534) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.48, 75.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (112, 278, 1175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3301) [M::mem_pestat] mean and std.dev: (436.28, 601.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4364) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (739, 1668, 2734) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6724) [M::mem_pestat] mean and std.dev: (1881.36, 1701.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8719) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 905.167 CPU sec, 50.970 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (57, 666950, 25, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (41, 91, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 443) [M::mem_pestat] mean and std.dev: (97.92, 70.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 577) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (216, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (12, 522) [M::mem_pestat] mean and std.dev: (269.45, 75.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 624) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 59, 689) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2005) [M::mem_pestat] mean and std.dev: (219.41, 459.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2663) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 917.069 CPU sec, 53.917 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (63, 667475, 33, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (36, 75, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 621) [M::mem_pestat] mean and std.dev: (99.07, 100.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 816) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.50, 75.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (47, 93, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 527) [M::mem_pestat] mean and std.dev: (77.15, 53.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 687) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 915.710 CPU sec, 40.647 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (68, 667224, 39, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (40, 84, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 415) [M::mem_pestat] mean and std.dev: (97.45, 73.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 540) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (11, 521) [M::mem_pestat] mean and std.dev: (269.27, 75.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 61, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (60.47, 46.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 637) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 910.599 CPU sec, 52.559 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (76, 667181, 31, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (37, 70, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 448) [M::mem_pestat] mean and std.dev: (97.44, 82.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.37, 75.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 79, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1210) [M::mem_pestat] mean and std.dev: (126.85, 174.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1601) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (938, 1290, 1945) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3959) [M::mem_pestat] mean and std.dev: (1454.80, 1083.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5790) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 918.623 CPU sec, 40.803 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (69, 666397, 31, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (41, 78, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (97.95, 74.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 617) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.34, 75.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (51, 83, 1591) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4671) [M::mem_pestat] mean and std.dev: (723.34, 1355.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6211) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 920.758 CPU sec, 54.863 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (56, 666886, 36, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (48, 112, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462) [M::mem_pestat] mean and std.dev: (112.53, 95.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 600) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.43, 75.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 82, 473) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1349) [M::mem_pestat] mean and std.dev: (156.19, 260.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1787) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (585, 787, 1611) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3663) [M::mem_pestat] mean and std.dev: (891.70, 547.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4689) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 912.507 CPU sec, 40.540 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (63, 666357, 31, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (39, 66, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 498) [M::mem_pestat] mean and std.dev: (94.12, 78.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 651) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.44, 75.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 79, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 482) [M::mem_pestat] mean and std.dev: (81.88, 94.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 634) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (525, 778, 1188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2514) [M::mem_pestat] mean and std.dev: (688.55, 414.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3177) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 909.580 CPU sec, 51.563 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (83, 666732, 31, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (45, 67, 139) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 327) [M::mem_pestat] mean and std.dev: (76.65, 56.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 421) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.44, 75.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 56, 351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 999) [M::mem_pestat] mean and std.dev: (88.16, 147.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1323) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (726, 1169, 2088) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4812) [M::mem_pestat] mean and std.dev: (1360.00, 988.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6174) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 902.807 CPU sec, 39.872 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (82, 667536, 30, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (40, 77, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 457) [M::mem_pestat] mean and std.dev: (98.07, 73.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 596) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.43, 75.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 91, 2171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6457) [M::mem_pestat] mean and std.dev: (839.64, 1471.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8600) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (814, 1097, 1535) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2977) [M::mem_pestat] mean and std.dev: (1086.69, 508.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3698) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 898.278 CPU sec, 50.124 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (86, 667398, 32, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (38, 71, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (90.61, 78.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 618) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.29, 75.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (56, 102, 404) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1100) [M::mem_pestat] mean and std.dev: (140.26, 167.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1448) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (211, 724, 1211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3211) [M::mem_pestat] mean and std.dev: (810.62, 761.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4211) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 901.961 CPU sec, 39.926 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (64, 667100, 20, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (40, 79, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 577) [M::mem_pestat] mean and std.dev: (117.02, 106.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 756) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.39, 75.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (59, 924, 4447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13223) [M::mem_pestat] mean and std.dev: (1591.45, 1897.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17611) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (626, 972, 2320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5708) [M::mem_pestat] mean and std.dev: (1406.69, 1023.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7402) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 900.142 CPU sec, 49.759 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (80, 666148, 22, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (40, 59, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (99.27, 91.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 632) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.25, 75.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 167, 2786) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8298) [M::mem_pestat] mean and std.dev: (1273.91, 1773.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11054) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (737, 899, 1176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2054) [M::mem_pestat] mean and std.dev: (756.57, 369.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2493) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 898.694 CPU sec, 39.647 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (57, 666051, 29, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (40, 83, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 571) [M::mem_pestat] mean and std.dev: (113.92, 109.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 748) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (11, 521) [M::mem_pestat] mean and std.dev: (269.20, 75.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (56, 147, 1539) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4505) [M::mem_pestat] mean and std.dev: (716.36, 1106.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5988) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (615, 1168, 1622) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3636) [M::mem_pestat] mean and std.dev: (1150.40, 633.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4643) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 909.910 CPU sec, 51.299 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (91, 666790, 29, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (46, 73, 156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 376) [M::mem_pestat] mean and std.dev: (83.85, 62.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 486) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.31, 75.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 140, 655) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1895) [M::mem_pestat] mean and std.dev: (306.96, 494.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2515) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (714, 1021, 4824) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13044) [M::mem_pestat] mean and std.dev: (2453.67, 2480.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17154) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 900.437 CPU sec, 39.785 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (91, 666952, 36, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (45, 71, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402) [M::mem_pestat] mean and std.dev: (87.47, 68.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 521) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.48, 75.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 63, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 452) [M::mem_pestat] mean and std.dev: (60.04, 53.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (834, 1038, 2097) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4623) [M::mem_pestat] mean and std.dev: (1242.83, 863.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5886) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 910.011 CPU sec, 51.891 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (69, 666847, 35, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (40, 66, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (88.54, 78.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 656) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (216, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (12, 522) [M::mem_pestat] mean and std.dev: (269.43, 75.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 624) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 31, 110) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 302) [M::mem_pestat] mean and std.dev: (45.23, 47.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 398) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 914.695 CPU sec, 53.901 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (65, 666753, 24, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (48, 99, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738) [M::mem_pestat] mean and std.dev: (141.98, 161.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 968) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.51, 75.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 79, 140) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 360) [M::mem_pestat] mean and std.dev: (61.95, 39.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 470) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (600, 774, 1428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3084) [M::mem_pestat] mean and std.dev: (894.77, 564.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3912) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 912.202 CPU sec, 40.620 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (71, 666621, 40, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (44, 80, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (90.52, 64.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 520) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.50, 75.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (44, 93, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713) [M::mem_pestat] mean and std.dev: (106.06, 111.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 936) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (622, 779, 1025) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1831) [M::mem_pestat] mean and std.dev: (744.25, 306.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2234) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 917.434 CPU sec, 53.871 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (76, 666147, 26, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (47, 107, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (111.09, 84.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 659) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.33, 75.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (46, 146, 3666) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10906) [M::mem_pestat] mean and std.dev: (1477.50, 2201.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14526) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (802, 1375, 1859) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3973) [M::mem_pestat] mean and std.dev: (1112.56, 698.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5030) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 914.965 CPU sec, 40.964 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (76, 666585, 22, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (42, 86, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (88.40, 64.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.31, 75.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (50, 104, 485) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1355) [M::mem_pestat] mean and std.dev: (126.59, 138.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1790) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (815, 948, 2924) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7142) [M::mem_pestat] mean and std.dev: (1242.80, 963.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9251) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 911.234 CPU sec, 52.750 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (76, 667082, 29, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (42, 70, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 429) [M::mem_pestat] mean and std.dev: (100.68, 90.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 558) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.31, 75.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 65, 1944) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5768) [M::mem_pestat] mean and std.dev: (842.46, 1406.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7680) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (660, 1064, 1609) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3507) [M::mem_pestat] mean and std.dev: (1120.95, 737.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4456) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 901.473 CPU sec, 39.954 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (92, 667381, 30, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (41, 80, 159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 395) [M::mem_pestat] mean and std.dev: (90.33, 68.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 513) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (11, 521) [M::mem_pestat] mean and std.dev: (269.17, 75.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 103, 132) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 342) [M::mem_pestat] mean and std.dev: (73.79, 65.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 447) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 903.799 CPU sec, 51.640 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (74, 667008, 36, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (38, 64, 133) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 323) [M::mem_pestat] mean and std.dev: (81.91, 66.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 418) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.51, 75.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 84, 1083) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3183) [M::mem_pestat] mean and std.dev: (514.40, 800.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4233) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (576, 1133, 4112) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11184) [M::mem_pestat] mean and std.dev: (2147.77, 2023.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14720) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 904.713 CPU sec, 40.150 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (68, 666741, 22, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (39, 83, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 465) [M::mem_pestat] mean and std.dev: (92.88, 69.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 607) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.48, 75.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 83, 386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1100) [M::mem_pestat] mean and std.dev: (144.89, 245.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1457) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (361, 837, 1104) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2590) [M::mem_pestat] mean and std.dev: (740.47, 457.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3333) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 896.751 CPU sec, 49.809 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (69, 666910, 37, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (43, 64, 152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 370) [M::mem_pestat] mean and std.dev: (89.21, 73.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 479) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.46, 75.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 93, 1497) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4437) [M::mem_pestat] mean and std.dev: (462.26, 902.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5907) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (989, 2530, 4640) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11942) [M::mem_pestat] mean and std.dev: (2548.67, 1916.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15593) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 902.852 CPU sec, 40.051 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (56, 666432, 26, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (42, 79, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 438) [M::mem_pestat] mean and std.dev: (97.83, 73.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 570) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.35, 75.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 88, 368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1062) [M::mem_pestat] mean and std.dev: (130.82, 197.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1409) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (582, 963, 1135) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2241) [M::mem_pestat] mean and std.dev: (802.00, 523.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2895) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 899.649 CPU sec, 50.193 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (75, 666901, 32, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (42, 60, 150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 366) [M::mem_pestat] mean and std.dev: (83.45, 67.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 474) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.40, 75.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 151, 1818) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5382) [M::mem_pestat] mean and std.dev: (1258.88, 1781.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8386) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (228, 960, 1749) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4791) [M::mem_pestat] mean and std.dev: (1025.23, 759.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6312) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 898.506 CPU sec, 39.751 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (76, 666511, 26, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (41, 75, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 419) [M::mem_pestat] mean and std.dev: (92.70, 72.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 545) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.42, 75.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 164, 1454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4302) [M::mem_pestat] mean and std.dev: (768.83, 1142.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5726) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 904.551 CPU sec, 50.725 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (63, 667188, 24, 23) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (40, 95, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406) [M::mem_pestat] mean and std.dev: (96.82, 66.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 528) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.55, 75.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (39, 114, 865) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2517) [M::mem_pestat] mean and std.dev: (315.00, 626.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3343) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (800, 1081, 1990) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4370) [M::mem_pestat] mean and std.dev: (1326.65, 858.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5560) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 896.307 CPU sec, 39.671 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (66, 666380, 25, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (39, 68, 142) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 348) [M::mem_pestat] mean and std.dev: (96.92, 79.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 451) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.43, 75.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 81, 1817) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5387) [M::mem_pestat] mean and std.dev: (749.48, 1241.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7172) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (823, 1137, 2066) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4552) [M::mem_pestat] mean and std.dev: (1368.36, 696.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5795) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 906.439 CPU sec, 49.121 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (69, 666592, 36, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (41, 74, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (101.98, 86.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 613) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.43, 75.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 85, 151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 389) [M::mem_pestat] mean and std.dev: (81.70, 81.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 508) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (475, 1048, 2264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5842) [M::mem_pestat] mean and std.dev: (1119.54, 810.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7631) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 899.458 CPU sec, 49.267 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (59, 667268, 19, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (41, 66, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 428) [M::mem_pestat] mean and std.dev: (95.26, 76.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 557) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.30, 75.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 59, 148) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 394) [M::mem_pestat] mean and std.dev: (54.20, 50.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 517) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (435, 761, 1445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3465) [M::mem_pestat] mean and std.dev: (888.50, 852.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4475) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 897.066 CPU sec, 39.491 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (60, 667215, 23, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (41, 70, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (103.13, 100.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 665) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.24, 75.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 46, 883) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2603) [M::mem_pestat] mean and std.dev: (243.11, 482.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3463) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (230, 1030, 1793) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4919) [M::mem_pestat] mean and std.dev: (1091.94, 836.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6482) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 900.424 CPU sec, 50.123 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (69, 666277, 29, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (38, 61, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (88.02, 77.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 542) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.32, 75.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (44, 104, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 392) [M::mem_pestat] mean and std.dev: (86.28, 54.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 508) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (499, 915, 1126) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2380) [M::mem_pestat] mean and std.dev: (742.58, 419.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3007) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 901.055 CPU sec, 39.862 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (72, 667440, 36, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (43, 106, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 517) [M::mem_pestat] mean and std.dev: (120.15, 91.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 675) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (216, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (12, 522) [M::mem_pestat] mean and std.dev: (269.45, 75.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 624) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (47, 122, 1585) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4661) [M::mem_pestat] mean and std.dev: (752.97, 1114.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6199) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 896.371 CPU sec, 49.744 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (73, 666594, 23, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (43, 72, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 508) [M::mem_pestat] mean and std.dev: (98.78, 89.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 663) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.54, 75.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 122, 331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923) [M::mem_pestat] mean and std.dev: (107.05, 113.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1219) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 899.792 CPU sec, 39.774 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (58, 666474, 22, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (39, 64, 158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 396) [M::mem_pestat] mean and std.dev: (83.12, 68.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 515) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.31, 75.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 83, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 599) [M::mem_pestat] mean and std.dev: (64.76, 59.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 790) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (717, 1015, 1856) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4134) [M::mem_pestat] mean and std.dev: (1124.00, 764.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5273) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 902.010 CPU sec, 49.904 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (61, 666751, 30, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (41, 73, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 431) [M::mem_pestat] mean and std.dev: (100.00, 82.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 561) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.44, 75.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (43, 122, 514) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1456) [M::mem_pestat] mean and std.dev: (142.46, 218.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1927) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (884, 1059, 1172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (308, 1748) [M::mem_pestat] mean and std.dev: (917.88, 218.79) [M::mem_pestat] low and high boundaries for proper pairs: (20, 2036) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 895.955 CPU sec, 39.628 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (75, 666402, 25, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (40, 79, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (98.50, 81.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 536) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.37, 75.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 72, 141) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 373) [M::mem_pestat] mean and std.dev: (68.38, 52.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 489) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (679, 1172, 2593) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6421) [M::mem_pestat] mean and std.dev: (1404.71, 1144.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8335) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 893.776 CPU sec, 48.280 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (70, 666879, 36, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (46, 86, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454) [M::mem_pestat] mean and std.dev: (105.00, 80.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.35, 75.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (56, 117, 411) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1121) [M::mem_pestat] mean and std.dev: (157.23, 199.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1476) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 901.200 CPU sec, 39.815 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (79, 666385, 34, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (51, 83, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573) [M::mem_pestat] mean and std.dev: (121.96, 117.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 747) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.45, 75.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (42, 101, 705) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2031) [M::mem_pestat] mean and std.dev: (237.41, 473.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2694) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (233, 926, 1423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3803) [M::mem_pestat] mean and std.dev: (840.62, 541.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4993) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 900.270 CPU sec, 49.983 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (78, 666715, 28, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (45, 86, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 525) [M::mem_pestat] mean and std.dev: (105.91, 90.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 685) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.36, 75.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 75, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 767) [M::mem_pestat] mean and std.dev: (81.57, 87.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1011) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (489, 1448, 2431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6315) [M::mem_pestat] mean and std.dev: (1261.94, 958.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8257) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 900.434 CPU sec, 39.833 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (82, 666345, 27, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (35, 90, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 563) [M::mem_pestat] mean and std.dev: (107.10, 99.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 739) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.46, 75.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (43, 81, 1975) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5839) [M::mem_pestat] mean and std.dev: (846.70, 1379.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7771) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (468, 1227, 1701) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4167) [M::mem_pestat] mean and std.dev: (1064.30, 671.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5400) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 895.639 CPU sec, 49.009 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (66, 666690, 31, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (52, 92, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 457) [M::mem_pestat] mean and std.dev: (100.33, 66.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 592) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (216, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (12, 522) [M::mem_pestat] mean and std.dev: (269.52, 75.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 624) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (45, 105, 1638) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4824) [M::mem_pestat] mean and std.dev: (842.53, 1476.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6748) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 902.416 CPU sec, 39.939 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (67, 666831, 22, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (39, 76, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 432) [M::mem_pestat] mean and std.dev: (95.34, 78.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 563) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.40, 75.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 75, 1669) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4971) [M::mem_pestat] mean and std.dev: (703.76, 1251.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6622) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (614, 954, 1252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2528) [M::mem_pestat] mean and std.dev: (941.27, 576.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3248) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 911.407 CPU sec, 46.963 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (68, 666526, 28, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (37, 58, 118) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (76.72, 58.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 361) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.45, 75.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (52, 154, 711) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2029) [M::mem_pestat] mean and std.dev: (306.20, 386.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2688) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (167, 1021, 1676) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4694) [M::mem_pestat] mean and std.dev: (909.00, 824.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6203) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 899.326 CPU sec, 50.644 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (63, 667197, 14, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (47, 79, 157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 377) [M::mem_pestat] mean and std.dev: (90.86, 61.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 487) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.47, 75.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (41, 150, 3065) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9113) [M::mem_pestat] mean and std.dev: (1703.57, 2472.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12137) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (593, 896, 1335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2819) [M::mem_pestat] mean and std.dev: (1066.00, 640.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3627) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 902.234 CPU sec, 40.051 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (60, 667158, 33, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (40, 63, 134) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 322) [M::mem_pestat] mean and std.dev: (76.34, 59.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 416) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.41, 75.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (49, 87, 629) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1789) [M::mem_pestat] mean and std.dev: (148.59, 303.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2369) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (720, 1744, 2322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5526) [M::mem_pestat] mean and std.dev: (1646.94, 1313.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7128) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 899.591 CPU sec, 50.265 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (73, 667078, 27, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (37, 82, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622) [M::mem_pestat] mean and std.dev: (100.66, 111.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 817) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.42, 75.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 52, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 495) [M::mem_pestat] mean and std.dev: (94.08, 115.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 652) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (527, 1496, 1917) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4697) [M::mem_pestat] mean and std.dev: (1000.67, 727.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6087) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 898.134 CPU sec, 39.844 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (72, 666550, 38, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (41, 77, 147) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 359) [M::mem_pestat] mean and std.dev: (84.08, 54.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 465) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.28, 75.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 75, 1834) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5444) [M::mem_pestat] mean and std.dev: (694.85, 1332.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7249) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (697, 928, 1055) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1771) [M::mem_pestat] mean and std.dev: (820.40, 324.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2129) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 893.984 CPU sec, 49.339 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (69, 666955, 28, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (37, 71, 156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 394) [M::mem_pestat] mean and std.dev: (99.17, 88.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 513) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.28, 75.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (50, 112, 2156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6368) [M::mem_pestat] mean and std.dev: (833.15, 1405.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8474) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (872, 975, 1461) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2639) [M::mem_pestat] mean and std.dev: (885.88, 532.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3228) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 894.768 CPU sec, 39.566 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (85, 667230, 33, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (36, 64, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 429) [M::mem_pestat] mean and std.dev: (80.33, 69.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 560) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.29, 75.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (95, 141, 1835) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5315) [M::mem_pestat] mean and std.dev: (849.57, 1412.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7055) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (514, 749, 1344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3004) [M::mem_pestat] mean and std.dev: (847.80, 568.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3834) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 899.053 CPU sec, 50.361 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (80, 666860, 27, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (43, 59, 156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 382) [M::mem_pestat] mean and std.dev: (90.29, 78.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 495) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.42, 75.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 64, 1816) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5378) [M::mem_pestat] mean and std.dev: (998.54, 1611.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7443) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (223, 846, 1421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3817) [M::mem_pestat] mean and std.dev: (988.67, 948.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5015) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 901.024 CPU sec, 39.810 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (80, 667264, 33, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (51, 92, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (102.95, 88.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 675) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.36, 75.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 95, 810) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2370) [M::mem_pestat] mean and std.dev: (130.26, 232.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3150) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (849, 989, 2278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5136) [M::mem_pestat] mean and std.dev: (1167.20, 600.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6565) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 900.256 CPU sec, 50.848 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (67, 667232, 20, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (47, 76, 158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 380) [M::mem_pestat] mean and std.dev: (91.20, 67.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 491) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.52, 75.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (48, 68, 748) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2148) [M::mem_pestat] mean and std.dev: (246.61, 397.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2848) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (98, 1064, 2296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6692) [M::mem_pestat] mean and std.dev: (1232.89, 1117.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8890) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 895.004 CPU sec, 39.523 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (74, 666763, 26, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (39, 55, 139) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 339) [M::mem_pestat] mean and std.dev: (77.32, 63.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 439) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.39, 75.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (52, 108, 944) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2728) [M::mem_pestat] mean and std.dev: (399.39, 682.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3620) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (167, 582, 1389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3833) [M::mem_pestat] mean and std.dev: (543.64, 500.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5055) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 895.323 CPU sec, 49.393 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (92, 666582, 37, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (45, 75, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 495) [M::mem_pestat] mean and std.dev: (101.51, 85.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 645) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.36, 75.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 97, 819) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2405) [M::mem_pestat] mean and std.dev: (121.93, 209.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3198) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (297, 659, 897) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2097) [M::mem_pestat] mean and std.dev: (591.11, 415.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2697) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 895.684 CPU sec, 39.643 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (75, 667072, 38, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (45, 79, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 495) [M::mem_pestat] mean and std.dev: (99.72, 85.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 645) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.27, 75.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 61, 127) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 313) [M::mem_pestat] mean and std.dev: (57.84, 40.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 406) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (832, 1297, 1692) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3412) [M::mem_pestat] mean and std.dev: (1281.15, 694.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4272) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 898.909 CPU sec, 50.333 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (74, 667505, 34, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (47, 80, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 542) [M::mem_pestat] mean and std.dev: (123.68, 117.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 707) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 262, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.26, 75.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 138, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678) [M::mem_pestat] mean and std.dev: (101.33, 106.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 894) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (656, 1248, 2130) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5078) [M::mem_pestat] mean and std.dev: (1171.50, 914.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6552) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 898.099 CPU sec, 39.733 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (70, 667130, 22, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (39, 66, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 435) [M::mem_pestat] mean and std.dev: (87.11, 72.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 567) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.41, 75.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (56, 144, 4234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12590) [M::mem_pestat] mean and std.dev: (1663.68, 2553.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16768) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 905.390 CPU sec, 43.792 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (74, 666622, 27, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (33, 69, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639) [M::mem_pestat] mean and std.dev: (125.01, 141.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 841) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (11, 521) [M::mem_pestat] mean and std.dev: (269.15, 75.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 74, 1588) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4722) [M::mem_pestat] mean and std.dev: (925.78, 1588.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7278) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (559, 815, 1433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3181) [M::mem_pestat] mean and std.dev: (893.54, 651.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4055) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 903.404 CPU sec, 49.980 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (70, 666595, 26, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (40, 65, 123) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 289) [M::mem_pestat] mean and std.dev: (82.09, 66.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 372) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.55, 75.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (43, 106, 871) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2527) [M::mem_pestat] mean and std.dev: (148.48, 243.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3355) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (903, 1312, 1564) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2886) [M::mem_pestat] mean and std.dev: (1029.08, 496.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3547) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 893.611 CPU sec, 39.408 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (64, 667393, 30, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (44, 63, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (98.33, 90.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 616) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.32, 75.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 68, 159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 419) [M::mem_pestat] mean and std.dev: (57.65, 42.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 549) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (181, 584, 971) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2551) [M::mem_pestat] mean and std.dev: (688.53, 577.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3341) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 894.518 CPU sec, 48.646 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (73, 667613, 24, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (47, 61, 122) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 272) [M::mem_pestat] mean and std.dev: (80.51, 56.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 347) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.37, 75.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (51, 111, 3358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9972) [M::mem_pestat] mean and std.dev: (1317.29, 2255.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13279) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (292, 755, 1399) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3613) [M::mem_pestat] mean and std.dev: (948.88, 790.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4720) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 895.463 CPU sec, 39.590 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (82, 666479, 26, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (44, 84, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 599) [M::mem_pestat] mean and std.dev: (113.94, 107.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 784) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.35, 75.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 51, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (75.57, 101.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 634) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (669, 823, 1819) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4119) [M::mem_pestat] mean and std.dev: (1120.27, 631.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5269) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 894.959 CPU sec, 49.041 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (79, 666822, 30, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (42, 79, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 540) [M::mem_pestat] mean and std.dev: (100.75, 105.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 706) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.50, 75.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (53, 103, 454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1256) [M::mem_pestat] mean and std.dev: (113.17, 147.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1657) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (755, 987, 1741) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3713) [M::mem_pestat] mean and std.dev: (1123.50, 781.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4699) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 893.547 CPU sec, 39.577 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (79, 666956, 36, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (47, 86, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 584) [M::mem_pestat] mean and std.dev: (101.65, 91.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 763) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.35, 75.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (67, 154, 2853) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8425) [M::mem_pestat] mean and std.dev: (1186.60, 1895.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11211) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (584, 743, 1984) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4784) [M::mem_pestat] mean and std.dev: (1202.00, 1199.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6184) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 897.674 CPU sec, 50.048 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (84, 666313, 24, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (42, 73, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 438) [M::mem_pestat] mean and std.dev: (89.72, 69.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 570) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (216, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (12, 522) [M::mem_pestat] mean and std.dev: (269.58, 75.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 624) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 134, 1669) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4939) [M::mem_pestat] mean and std.dev: (852.79, 1407.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6574) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 904.074 CPU sec, 40.142 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (60, 666017, 25, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (45, 110, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 489) [M::mem_pestat] mean and std.dev: (111.07, 85.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 637) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.43, 75.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (48, 113, 2957) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8775) [M::mem_pestat] mean and std.dev: (1113.28, 1659.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11684) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (551, 821, 2825) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7373) [M::mem_pestat] mean and std.dev: (1174.62, 1102.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9647) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 904.149 CPU sec, 50.691 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (83, 666709, 20, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (44, 70, 155) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 377) [M::mem_pestat] mean and std.dev: (96.47, 79.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 488) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.52, 75.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (39, 111, 147) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 363) [M::mem_pestat] mean and std.dev: (83.00, 61.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 471) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 897.934 CPU sec, 39.716 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (67, 667046, 33, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (43, 79, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (131.61, 130.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 779) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.35, 75.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 63, 2080) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6182) [M::mem_pestat] mean and std.dev: (806.97, 1479.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8233) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (478, 671, 2157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5515) [M::mem_pestat] mean and std.dev: (1028.12, 944.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7194) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 896.494 CPU sec, 50.043 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (81, 667192, 30, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (37, 59, 116) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 274) [M::mem_pestat] mean and std.dev: (70.11, 51.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 353) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.44, 75.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 63, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 994) [M::mem_pestat] mean and std.dev: (104.29, 157.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1315) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (468, 918, 2527) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6645) [M::mem_pestat] mean and std.dev: (1441.07, 1365.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8704) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 897.037 CPU sec, 39.788 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (81, 666500, 34, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (41, 64, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 545) [M::mem_pestat] mean and std.dev: (93.16, 87.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 713) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.48, 75.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (59, 129, 1407) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4103) [M::mem_pestat] mean and std.dev: (573.40, 1016.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5451) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (844, 911, 1115) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (302, 1657) [M::mem_pestat] mean and std.dev: (953.44, 143.06) [M::mem_pestat] low and high boundaries for proper pairs: (31, 1928) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 896.288 CPU sec, 49.447 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (69, 667060, 22, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (37, 74, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 562) [M::mem_pestat] mean and std.dev: (109.41, 99.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 737) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.46, 75.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (40, 130, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (86.71, 52.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 548) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (243, 1149, 2290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6384) [M::mem_pestat] mean and std.dev: (1101.67, 906.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8431) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 898.401 CPU sec, 39.673 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (84, 666987, 21, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (36, 69, 119) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 285) [M::mem_pestat] mean and std.dev: (77.43, 56.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 368) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 262, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.21, 75.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (55, 153, 1166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3388) [M::mem_pestat] mean and std.dev: (351.47, 451.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4499) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (492, 859, 1230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2706) [M::mem_pestat] mean and std.dev: (861.60, 476.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3444) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 904.687 CPU sec, 41.787 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (88, 666709, 33, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (44, 97, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 614) [M::mem_pestat] mean and std.dev: (118.40, 96.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 804) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.45, 75.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (48, 201, 3239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9621) [M::mem_pestat] mean and std.dev: (1497.76, 1981.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12812) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (245, 1736, 3405) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9725) [M::mem_pestat] mean and std.dev: (2109.57, 2119.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12885) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 904.020 CPU sec, 51.647 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (88, 667191, 28, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (46, 103, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (115.91, 104.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 654) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.41, 75.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 61, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1274) [M::mem_pestat] mean and std.dev: (71.09, 91.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1688) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 899.386 CPU sec, 39.744 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (84, 666425, 29, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (39, 63, 123) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 291) [M::mem_pestat] mean and std.dev: (68.21, 46.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 375) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (216, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (12, 522) [M::mem_pestat] mean and std.dev: (269.40, 75.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 624) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (72, 128, 1271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3669) [M::mem_pestat] mean and std.dev: (404.00, 675.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4868) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (571, 789, 815) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (83, 1303) [M::mem_pestat] mean and std.dev: (727.80, 197.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1547) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 904.909 CPU sec, 50.932 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (76, 666883, 26, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (43, 75, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463) [M::mem_pestat] mean and std.dev: (99.51, 85.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.36, 75.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (45, 140, 1796) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5298) [M::mem_pestat] mean and std.dev: (982.64, 1616.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7448) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (546, 1115, 1417) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3159) [M::mem_pestat] mean and std.dev: (906.77, 502.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4030) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 901.073 CPU sec, 40.093 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (84, 666804, 24, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (39, 61, 157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 393) [M::mem_pestat] mean and std.dev: (91.16, 71.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 511) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.41, 75.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 55, 2249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6717) [M::mem_pestat] mean and std.dev: (974.96, 1816.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8951) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 896.283 CPU sec, 49.411 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (84, 667055, 36, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (48, 74, 153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 363) [M::mem_pestat] mean and std.dev: (100.17, 75.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 468) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.47, 75.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (46, 103, 894) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2590) [M::mem_pestat] mean and std.dev: (229.47, 439.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3438) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (736, 896, 2621) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6391) [M::mem_pestat] mean and std.dev: (1523.00, 1374.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8276) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 897.731 CPU sec, 39.696 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (72, 667015, 36, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (45, 82, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663) [M::mem_pestat] mean and std.dev: (105.67, 96.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 869) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.19, 75.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 106, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1243) [M::mem_pestat] mean and std.dev: (107.90, 122.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1646) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1142, 1524, 2154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4178) [M::mem_pestat] mean and std.dev: (1593.29, 664.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5190) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 898.517 CPU sec, 50.459 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (66, 666318, 22, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (37, 73, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 445) [M::mem_pestat] mean and std.dev: (91.42, 75.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 581) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.40, 75.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (40, 172, 2790) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8290) [M::mem_pestat] mean and std.dev: (1388.73, 1861.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11040) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (681, 984, 1622) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3504) [M::mem_pestat] mean and std.dev: (1317.62, 996.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5303) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 896.583 CPU sec, 39.686 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (72, 667309, 31, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (45, 74, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 498) [M::mem_pestat] mean and std.dev: (109.77, 103.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 649) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.33, 75.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (52, 159, 1790) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5266) [M::mem_pestat] mean and std.dev: (1010.80, 1358.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7004) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (520, 1229, 2412) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6196) [M::mem_pestat] mean and std.dev: (1433.25, 1467.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8088) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 898.311 CPU sec, 50.510 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (79, 666738, 35, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (42, 77, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 456) [M::mem_pestat] mean and std.dev: (92.61, 69.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.43, 75.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (43, 88, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 394) [M::mem_pestat] mean and std.dev: (71.00, 47.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 511) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 894.472 CPU sec, 39.570 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (87, 667067, 22, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (32, 50, 129) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 323) [M::mem_pestat] mean and std.dev: (71.50, 59.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 420) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.47, 75.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 64, 120) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 316) [M::mem_pestat] mean and std.dev: (57.37, 42.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 414) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (545, 698, 1170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2420) [M::mem_pestat] mean and std.dev: (594.00, 313.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3045) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 895.482 CPU sec, 50.731 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (78, 667174, 32, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (43, 77, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643) [M::mem_pestat] mean and std.dev: (105.97, 119.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 843) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.29, 75.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (77, 152, 4545) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13481) [M::mem_pestat] mean and std.dev: (1815.88, 2659.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17949) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 894.462 CPU sec, 39.560 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (60, 666669, 28, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (33, 58, 149) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 381) [M::mem_pestat] mean and std.dev: (73.94, 65.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 497) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.42, 75.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 63, 1321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3905) [M::mem_pestat] mean and std.dev: (500.72, 932.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5197) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (778, 1265, 1921) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4207) [M::mem_pestat] mean and std.dev: (1341.50, 955.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5350) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 895.100 CPU sec, 49.965 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (78, 666918, 24, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (40, 62, 131) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 313) [M::mem_pestat] mean and std.dev: (82.15, 64.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 404) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.31, 75.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 105, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492) [M::mem_pestat] mean and std.dev: (100.30, 97.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 645) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (532, 1228, 1705) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4051) [M::mem_pestat] mean and std.dev: (1265.15, 993.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5239) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 900.639 CPU sec, 39.755 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (76, 667410, 23, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (40, 77, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (95.14, 72.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 536) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.47, 75.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 94, 634) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1870) [M::mem_pestat] mean and std.dev: (208.80, 368.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2488) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (700, 1011, 1571) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3313) [M::mem_pestat] mean and std.dev: (1007.55, 711.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4184) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 896.299 CPU sec, 48.737 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (83, 666609, 28, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (41, 71, 154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 380) [M::mem_pestat] mean and std.dev: (85.34, 63.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 493) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.42, 75.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 60, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915) [M::mem_pestat] mean and std.dev: (76.95, 85.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1209) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 895.472 CPU sec, 39.635 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (81, 666862, 28, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (35, 54, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (79.20, 73.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 619) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 262, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.22, 75.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (38, 831, 2674) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7946) [M::mem_pestat] mean and std.dev: (1210.50, 1445.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10582) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (666, 963, 1070) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1878) [M::mem_pestat] mean and std.dev: (680.27, 344.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2282) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 895.756 CPU sec, 50.019 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (76, 667096, 24, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (36, 61, 159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (81.69, 66.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 528) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.48, 75.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 35, 115) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 301) [M::mem_pestat] mean and std.dev: (55.36, 47.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 394) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (655, 1013, 2080) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4930) [M::mem_pestat] mean and std.dev: (1205.50, 949.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6355) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 895.445 CPU sec, 39.678 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (72, 666967, 40, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (46, 83, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 397) [M::mem_pestat] mean and std.dev: (100.73, 76.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 514) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.43, 75.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 53, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463) [M::mem_pestat] mean and std.dev: (53.97, 45.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 895.334 CPU sec, 50.629 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (76, 667069, 28, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (46, 64, 131) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 301) [M::mem_pestat] mean and std.dev: (77.74, 52.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 386) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.45, 75.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (39, 110, 791) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2295) [M::mem_pestat] mean and std.dev: (368.33, 506.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3047) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (866, 1409, 2015) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4313) [M::mem_pestat] mean and std.dev: (1334.38, 662.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5462) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 898.855 CPU sec, 39.730 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (63, 666882, 37, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (39, 72, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (79.69, 64.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 543) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.48, 75.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (46, 119, 1974) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5830) [M::mem_pestat] mean and std.dev: (772.61, 1166.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7758) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (622, 1039, 1424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3028) [M::mem_pestat] mean and std.dev: (1038.47, 749.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4035) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 899.845 CPU sec, 50.601 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (86, 667154, 27, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (43, 70, 158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388) [M::mem_pestat] mean and std.dev: (87.32, 63.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 503) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 262, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.29, 75.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (43, 84, 1646) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4852) [M::mem_pestat] mean and std.dev: (781.22, 1277.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6455) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (169, 528, 770) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1972) [M::mem_pestat] mean and std.dev: (553.42, 425.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2573) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 895.710 CPU sec, 39.648 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (64, 667090, 39, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (40, 68, 146) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 358) [M::mem_pestat] mean and std.dev: (88.31, 71.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 464) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.38, 75.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 101, 1124) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3302) [M::mem_pestat] mean and std.dev: (288.03, 570.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4391) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (590, 1081, 1576) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3548) [M::mem_pestat] mean and std.dev: (1170.00, 699.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4534) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 898.193 CPU sec, 50.133 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (80, 666570, 25, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (40, 75, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 580) [M::mem_pestat] mean and std.dev: (102.14, 99.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 760) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.40, 75.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 109, 2776) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8268) [M::mem_pestat] mean and std.dev: (1106.48, 1683.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11014) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (235, 870, 1677) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4561) [M::mem_pestat] mean and std.dev: (1000.50, 853.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6003) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 898.817 CPU sec, 39.641 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (64, 666383, 37, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (47, 126, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671) [M::mem_pestat] mean and std.dev: (129.31, 118.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 879) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.41, 75.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 117, 1614) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4780) [M::mem_pestat] mean and std.dev: (693.83, 1171.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6363) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (923, 1131, 2521) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5717) [M::mem_pestat] mean and std.dev: (1281.23, 838.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7315) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 898.008 CPU sec, 51.314 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (88, 666794, 38, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (39, 63, 126) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 300) [M::mem_pestat] mean and std.dev: (74.81, 55.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 387) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.61, 75.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 78, 333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929) [M::mem_pestat] mean and std.dev: (103.06, 122.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1227) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (539, 830, 1960) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4802) [M::mem_pestat] mean and std.dev: (1272.52, 1037.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6223) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 899.800 CPU sec, 39.836 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (80, 667051, 33, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (42, 89, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 555) [M::mem_pestat] mean and std.dev: (114.15, 105.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 726) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.38, 75.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 72, 871) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2551) [M::mem_pestat] mean and std.dev: (400.77, 631.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3391) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (631, 692, 898) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (97, 1432) [M::mem_pestat] mean and std.dev: (732.88, 317.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2003) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 897.131 CPU sec, 49.635 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (74, 666806, 26, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (43, 83, 158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388) [M::mem_pestat] mean and std.dev: (91.93, 65.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 503) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.34, 75.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 44, 154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 398) [M::mem_pestat] mean and std.dev: (60.14, 52.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 520) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (460, 877, 1153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2539) [M::mem_pestat] mean and std.dev: (772.50, 578.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3232) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 898.501 CPU sec, 39.588 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 666456, 38, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (40, 61, 154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 382) [M::mem_pestat] mean and std.dev: (90.56, 73.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 496) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.34, 75.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 106, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1234) [M::mem_pestat] mean and std.dev: (104.63, 115.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1633) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (604, 996, 1569) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3499) [M::mem_pestat] mean and std.dev: (1008.69, 530.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4464) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 898.382 CPU sec, 51.561 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (82, 666797, 35, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (41, 66, 149) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 365) [M::mem_pestat] mean and std.dev: (78.77, 63.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 473) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (11, 521) [M::mem_pestat] mean and std.dev: (269.22, 75.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 82, 143) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 355) [M::mem_pestat] mean and std.dev: (83.94, 64.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (765, 1076, 1538) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3084) [M::mem_pestat] mean and std.dev: (1085.25, 524.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3857) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 896.486 CPU sec, 51.009 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (60, 667381, 35, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (45, 83, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594) [M::mem_pestat] mean and std.dev: (114.12, 104.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 777) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.57, 75.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 68, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 442) [M::mem_pestat] mean and std.dev: (55.89, 44.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 580) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (784, 1032, 1296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2320) [M::mem_pestat] mean and std.dev: (875.89, 325.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2832) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 897.251 CPU sec, 39.810 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (81, 666562, 25, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (38, 63, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (97.49, 88.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 642) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.42, 75.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (38, 53, 152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 380) [M::mem_pestat] mean and std.dev: (72.86, 54.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 494) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (742, 917, 1540) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3136) [M::mem_pestat] mean and std.dev: (823.07, 451.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3934) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 895.809 CPU sec, 50.340 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (76, 666845, 29, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (36, 58, 126) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 306) [M::mem_pestat] mean and std.dev: (76.38, 59.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 396) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.43, 75.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 67, 385) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1081) [M::mem_pestat] mean and std.dev: (88.13, 102.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1429) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (204, 724, 1599) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4389) [M::mem_pestat] mean and std.dev: (1206.50, 1174.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5905) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 899.354 CPU sec, 39.791 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (91, 666730, 31, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (41, 66, 140) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 338) [M::mem_pestat] mean and std.dev: (88.37, 68.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 437) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.37, 75.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (46, 77, 1743) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5137) [M::mem_pestat] mean and std.dev: (945.03, 1507.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6976) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (346, 746, 1198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2902) [M::mem_pestat] mean and std.dev: (943.93, 781.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4071) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 894.663 CPU sec, 50.777 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (71, 667328, 42, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (33, 48, 119) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 291) [M::mem_pestat] mean and std.dev: (64.11, 54.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 377) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (216, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (12, 522) [M::mem_pestat] mean and std.dev: (269.50, 75.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 624) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (43, 104, 1305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3829) [M::mem_pestat] mean and std.dev: (410.58, 885.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5091) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (374, 658, 1396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3440) [M::mem_pestat] mean and std.dev: (787.18, 561.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4462) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 895.968 CPU sec, 39.695 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (75, 666898, 19, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (43, 65, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 520) [M::mem_pestat] mean and std.dev: (94.61, 88.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 679) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.48, 75.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 78, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 430) [M::mem_pestat] mean and std.dev: (69.33, 55.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 562) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (817, 1025, 1335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2371) [M::mem_pestat] mean and std.dev: (1094.90, 471.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2980) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 905.485 CPU sec, 52.332 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (57, 666427, 33, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (46, 74, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 451) [M::mem_pestat] mean and std.dev: (99.52, 82.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.34, 75.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 94, 413) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1165) [M::mem_pestat] mean and std.dev: (116.96, 191.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1541) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 906.386 CPU sec, 40.223 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (86, 666618, 29, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (41, 61, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458) [M::mem_pestat] mean and std.dev: (94.70, 81.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.26, 75.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 74, 635) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1851) [M::mem_pestat] mean and std.dev: (202.96, 386.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2459) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 895.556 CPU sec, 50.529 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (75, 666956, 30, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (52, 82, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 397) [M::mem_pestat] mean and std.dev: (96.48, 61.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 512) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.48, 75.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 108, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 587) [M::mem_pestat] mean and std.dev: (75.65, 60.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 773) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 898, 1006) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2636) [M::mem_pestat] mean and std.dev: (713.90, 441.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3451) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 897.126 CPU sec, 39.715 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (73, 666587, 28, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (39, 54, 131) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 315) [M::mem_pestat] mean and std.dev: (77.85, 60.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 407) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.45, 75.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (44, 304, 1943) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5741) [M::mem_pestat] mean and std.dev: (931.92, 1411.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7640) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (690, 844, 1661) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3603) [M::mem_pestat] mean and std.dev: (1032.92, 648.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4574) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 899.511 CPU sec, 50.481 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (64, 666736, 30, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (41, 94, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 680) [M::mem_pestat] mean and std.dev: (117.35, 115.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 893) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.51, 75.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (62, 135, 1110) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3206) [M::mem_pestat] mean and std.dev: (428.08, 675.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4254) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (936, 1126, 1465) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2523) [M::mem_pestat] mean and std.dev: (1104.31, 441.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3052) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 892.198 CPU sec, 39.500 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (66, 666583, 28, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (35, 54, 127) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 311) [M::mem_pestat] mean and std.dev: (70.49, 54.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 403) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.39, 75.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 80, 141) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 357) [M::mem_pestat] mean and std.dev: (68.39, 45.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 465) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (652, 759, 1904) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4408) [M::mem_pestat] mean and std.dev: (1137.60, 1209.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5977) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 898.525 CPU sec, 49.447 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (65, 667162, 22, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (38, 64, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 563) [M::mem_pestat] mean and std.dev: (101.04, 97.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 738) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.52, 75.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 130, 4365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13021) [M::mem_pestat] mean and std.dev: (1683.41, 2517.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17349) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (751, 962, 1505) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3013) [M::mem_pestat] mean and std.dev: (973.31, 657.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3767) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 897.203 CPU sec, 39.704 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (84, 667112, 29, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (40, 62, 127) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 301) [M::mem_pestat] mean and std.dev: (81.55, 59.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 388) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.33, 75.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (40, 105, 593) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1699) [M::mem_pestat] mean and std.dev: (217.36, 388.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2252) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 895.782 CPU sec, 49.897 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (68, 667105, 33, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (47, 76, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 482) [M::mem_pestat] mean and std.dev: (91.55, 73.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.46, 75.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 63, 377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1061) [M::mem_pestat] mean and std.dev: (111.46, 151.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1403) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1074, 1188, 1491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (240, 2325) [M::mem_pestat] mean and std.dev: (1131.11, 295.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2742) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 903.635 CPU sec, 51.585 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (80, 667006, 23, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (42, 74, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570) [M::mem_pestat] mean and std.dev: (94.97, 89.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 746) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.30, 75.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 87, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 440) [M::mem_pestat] mean and std.dev: (62.94, 51.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 577) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 902.783 CPU sec, 40.087 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (71, 667232, 26, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (39, 56, 128) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 306) [M::mem_pestat] mean and std.dev: (73.68, 57.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 395) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.40, 75.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (40, 128, 1029) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3007) [M::mem_pestat] mean and std.dev: (408.17, 633.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3996) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1167, 2069, 3663) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8655) [M::mem_pestat] mean and std.dev: (2671.94, 2195.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11452) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 903.330 CPU sec, 52.275 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (65, 667093, 35, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (43, 68, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 571) [M::mem_pestat] mean and std.dev: (113.61, 111.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 747) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.34, 75.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (40, 126, 2916) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8668) [M::mem_pestat] mean and std.dev: (995.26, 1603.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11544) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 901.346 CPU sec, 39.993 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (77, 666541, 28, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (39, 59, 125) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 297) [M::mem_pestat] mean and std.dev: (73.50, 57.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 383) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.33, 75.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (44, 101, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713) [M::mem_pestat] mean and std.dev: (78.32, 71.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 936) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (637, 928, 1265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2521) [M::mem_pestat] mean and std.dev: (885.42, 380.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3149) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 906.081 CPU sec, 53.740 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (75, 666803, 27, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (42, 75, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 579) [M::mem_pestat] mean and std.dev: (114.24, 111.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 758) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.34, 75.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 58, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (72.14, 87.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 650) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (216, 1485, 1864) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5160) [M::mem_pestat] mean and std.dev: (1344.86, 1143.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6808) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 899.399 CPU sec, 40.008 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (75, 666559, 36, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (41, 64, 125) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 293) [M::mem_pestat] mean and std.dev: (72.03, 47.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 377) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.39, 75.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (48, 109, 1053) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3063) [M::mem_pestat] mean and std.dev: (388.66, 681.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4068) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (679, 1417, 1738) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3856) [M::mem_pestat] mean and std.dev: (997.64, 660.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4915) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 905.582 CPU sec, 52.581 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (90, 666574, 21, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (43, 82, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (81.64, 53.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 531) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (216, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (12, 522) [M::mem_pestat] mean and std.dev: (269.59, 75.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 624) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 66, 150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 382) [M::mem_pestat] mean and std.dev: (56.41, 43.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 498) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (561, 855, 2133) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5277) [M::mem_pestat] mean and std.dev: (1062.18, 810.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6849) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 901.814 CPU sec, 39.981 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (88, 666644, 28, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (43, 69, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 430) [M::mem_pestat] mean and std.dev: (83.80, 66.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 559) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.40, 75.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 53, 157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 429) [M::mem_pestat] mean and std.dev: (53.35, 51.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 565) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (787, 1523, 3453) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8785) [M::mem_pestat] mean and std.dev: (1770.75, 1297.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11451) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 905.838 CPU sec, 53.002 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (77, 666475, 24, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (40, 79, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (97.50, 73.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 652) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.31, 75.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (44, 140, 1706) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5030) [M::mem_pestat] mean and std.dev: (968.17, 1531.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7095) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 896.049 CPU sec, 39.733 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (75, 666803, 22, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (40, 70, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 553) [M::mem_pestat] mean and std.dev: (111.50, 105.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 724) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.45, 75.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 138, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866) [M::mem_pestat] mean and std.dev: (109.67, 96.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1144) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (536, 909, 1393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3107) [M::mem_pestat] mean and std.dev: (1079.60, 780.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4203) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 901.408 CPU sec, 50.826 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (86, 666361, 39, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (39, 63, 140) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 342) [M::mem_pestat] mean and std.dev: (88.04, 72.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 443) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.59, 75.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 58, 157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (63.47, 57.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 538) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (751, 942, 1871) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4111) [M::mem_pestat] mean and std.dev: (1069.15, 667.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5231) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 896.605 CPU sec, 39.677 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (66, 667126, 26, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (33, 57, 113) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 273) [M::mem_pestat] mean and std.dev: (66.83, 45.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 353) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.33, 75.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 136, 1774) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5258) [M::mem_pestat] mean and std.dev: (1325.00, 1881.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8852) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (747, 1258, 2416) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5754) [M::mem_pestat] mean and std.dev: (1390.09, 870.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7423) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 899.453 CPU sec, 51.087 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (89, 666828, 28, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (40, 78, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 556) [M::mem_pestat] mean and std.dev: (97.60, 80.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 728) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.40, 75.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (55, 83, 869) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2497) [M::mem_pestat] mean and std.dev: (262.92, 478.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3311) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (850, 1439, 2502) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5806) [M::mem_pestat] mean and std.dev: (1415.16, 796.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7458) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 902.629 CPU sec, 40.067 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (74, 666102, 26, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (38, 84, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 431) [M::mem_pestat] mean and std.dev: (95.78, 76.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 562) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.41, 75.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 79, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 727) [M::mem_pestat] mean and std.dev: (85.90, 93.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 960) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1018, 1097, 1515) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (24, 2509) [M::mem_pestat] mean and std.dev: (955.88, 438.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3006) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 918.688 CPU sec, 52.270 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (79, 666094, 38, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (44, 68, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 434) [M::mem_pestat] mean and std.dev: (95.34, 82.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 564) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.41, 75.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 68, 158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 434) [M::mem_pestat] mean and std.dev: (62.52, 53.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 572) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 900.894 CPU sec, 51.234 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (75, 666118, 27, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (45, 78, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 498) [M::mem_pestat] mean and std.dev: (101.02, 88.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 649) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.45, 75.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (73, 147, 1737) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5065) [M::mem_pestat] mean and std.dev: (988.11, 1498.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6981) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 893.173 CPU sec, 39.490 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (76, 666959, 30, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (42, 77, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 465) [M::mem_pestat] mean and std.dev: (87.51, 64.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.36, 75.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 101, 1648) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4920) [M::mem_pestat] mean and std.dev: (692.31, 1070.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6556) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (481, 788, 1624) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3910) [M::mem_pestat] mean and std.dev: (937.24, 707.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5053) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 901.309 CPU sec, 50.919 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (69, 667114, 36, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (41, 62, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 425) [M::mem_pestat] mean and std.dev: (97.63, 90.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 553) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.59, 75.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (64, 134, 466) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1270) [M::mem_pestat] mean and std.dev: (161.00, 215.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1672) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (591, 915, 2379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5955) [M::mem_pestat] mean and std.dev: (1249.58, 1031.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7743) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 894.040 CPU sec, 39.673 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (80, 666823, 27, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (52, 105, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667) [M::mem_pestat] mean and std.dev: (127.33, 129.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 872) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.38, 75.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (57, 146, 2991) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8859) [M::mem_pestat] mean and std.dev: (1354.48, 1918.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11793) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 903.421 CPU sec, 52.502 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (78, 666705, 34, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (45, 57, 136) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 318) [M::mem_pestat] mean and std.dev: (84.88, 65.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 409) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.37, 75.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 100, 554) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1592) [M::mem_pestat] mean and std.dev: (151.00, 225.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2111) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (255, 699, 1060) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2670) [M::mem_pestat] mean and std.dev: (637.71, 392.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3475) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 899.662 CPU sec, 39.945 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (73, 666731, 25, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (32, 51, 156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 404) [M::mem_pestat] mean and std.dev: (90.13, 81.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 528) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.44, 75.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 121, 584) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1704) [M::mem_pestat] mean and std.dev: (181.71, 305.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2264) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (542, 984, 2181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5459) [M::mem_pestat] mean and std.dev: (1214.91, 940.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7098) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 906.126 CPU sec, 52.731 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (66, 667100, 29, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (51, 87, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 459) [M::mem_pestat] mean and std.dev: (100.05, 69.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 595) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.44, 75.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 101, 2158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6418) [M::mem_pestat] mean and std.dev: (1066.41, 1667.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8548) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (548, 890, 1296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2792) [M::mem_pestat] mean and std.dev: (891.87, 614.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3540) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 900.910 CPU sec, 39.869 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (62, 666893, 24, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (31, 63, 117) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 289) [M::mem_pestat] mean and std.dev: (70.22, 56.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 375) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.35, 75.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 44, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 435) [M::mem_pestat] mean and std.dev: (63.81, 68.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 571) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (412, 977, 1330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3166) [M::mem_pestat] mean and std.dev: (942.83, 685.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4084) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 905.039 CPU sec, 52.126 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (68, 666764, 27, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (47, 74, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 398) [M::mem_pestat] mean and std.dev: (83.61, 58.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 515) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.52, 75.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 58, 89) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 195) [M::mem_pestat] mean and std.dev: (57.76, 36.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 248) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (416, 922, 1867) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4769) [M::mem_pestat] mean and std.dev: (1146.42, 1118.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6220) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 899.530 CPU sec, 39.921 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (81, 666597, 20, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (37, 78, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (92.47, 76.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 621) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.38, 75.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (43, 54, 3842) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11440) [M::mem_pestat] mean and std.dev: (1386.95, 2183.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15239) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (587, 1267, 2101) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5129) [M::mem_pestat] mean and std.dev: (1044.79, 699.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6643) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 905.703 CPU sec, 51.777 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (90, 666875, 29, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (40, 52, 127) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 301) [M::mem_pestat] mean and std.dev: (81.74, 64.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 388) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.40, 75.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 42, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1255) [M::mem_pestat] mean and std.dev: (99.21, 138.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1668) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (712, 1274, 3612) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9412) [M::mem_pestat] mean and std.dev: (2221.18, 2475.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12312) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 897.428 CPU sec, 39.669 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (74, 666895, 37, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (40, 55, 145) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 355) [M::mem_pestat] mean and std.dev: (80.41, 63.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 460) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.20, 75.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (40, 97, 587) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1681) [M::mem_pestat] mean and std.dev: (127.37, 189.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2228) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (707, 889, 1157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2057) [M::mem_pestat] mean and std.dev: (824.57, 351.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2507) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 903.894 CPU sec, 51.559 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (78, 667356, 24, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (38, 60, 139) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 341) [M::mem_pestat] mean and std.dev: (85.71, 69.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 442) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.50, 75.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (42, 98, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663) [M::mem_pestat] mean and std.dev: (114.62, 116.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 870) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (762, 1352, 2361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5559) [M::mem_pestat] mean and std.dev: (1469.21, 880.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7158) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 896.267 CPU sec, 39.756 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (85, 667108, 25, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (44, 67, 143) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 341) [M::mem_pestat] mean and std.dev: (86.21, 71.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 440) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (11, 521) [M::mem_pestat] mean and std.dev: (269.20, 75.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (50, 113, 1549) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4547) [M::mem_pestat] mean and std.dev: (567.57, 920.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6046) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (735, 1165, 1999) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4527) [M::mem_pestat] mean and std.dev: (1174.08, 748.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5791) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 910.719 CPU sec, 50.835 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (67, 666980, 15, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (47, 97, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458) [M::mem_pestat] mean and std.dev: (109.23, 85.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 595) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 262, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (11, 521) [M::mem_pestat] mean and std.dev: (269.08, 75.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (59, 88, 2604) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7694) [M::mem_pestat] mean and std.dev: (998.00, 1595.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10239) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (753, 1105, 1692) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3570) [M::mem_pestat] mean and std.dev: (1160.00, 607.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4509) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 906.285 CPU sec, 52.317 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (77, 666124, 34, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (38, 73, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (94.90, 78.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.47, 75.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (47, 129, 1225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3581) [M::mem_pestat] mean and std.dev: (314.07, 583.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4759) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 900.901 CPU sec, 39.841 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (86, 666939, 28, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (44, 68, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 392) [M::mem_pestat] mean and std.dev: (91.22, 65.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 508) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.41, 75.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (47, 74, 868) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2510) [M::mem_pestat] mean and std.dev: (350.77, 602.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3331) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (930, 1021, 1617) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2991) [M::mem_pestat] mean and std.dev: (1123.58, 374.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3678) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 905.240 CPU sec, 52.799 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (78, 666503, 31, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (39, 69, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 489) [M::mem_pestat] mean and std.dev: (78.24, 58.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 639) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.33, 75.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 124, 1205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3541) [M::mem_pestat] mean and std.dev: (573.28, 936.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4709) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (907, 1321, 1925) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3961) [M::mem_pestat] mean and std.dev: (1175.55, 623.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4979) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 902.035 CPU sec, 39.929 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (73, 666932, 22, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (46, 73, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634) [M::mem_pestat] mean and std.dev: (114.47, 117.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 830) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.39, 75.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (58, 132, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 391) [M::mem_pestat] mean and std.dev: (83.53, 50.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 502) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (524, 1190, 2799) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7349) [M::mem_pestat] mean and std.dev: (1649.76, 1512.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9624) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 911.570 CPU sec, 53.333 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (76, 667286, 45, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (42, 83, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570) [M::mem_pestat] mean and std.dev: (124.07, 115.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 746) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.51, 75.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 113, 1123) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3305) [M::mem_pestat] mean and std.dev: (408.77, 781.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4396) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (880, 1219, 3210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7870) [M::mem_pestat] mean and std.dev: (2088.42, 2274.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11185) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 903.813 CPU sec, 39.896 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (64, 667093, 29, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (41, 96, 156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 386) [M::mem_pestat] mean and std.dev: (93.11, 67.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 501) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.38, 75.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 74, 793) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2317) [M::mem_pestat] mean and std.dev: (212.50, 448.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3079) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 904.776 CPU sec, 53.223 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (73, 666482, 26, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (41, 64, 148) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 362) [M::mem_pestat] mean and std.dev: (84.36, 66.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 469) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.50, 75.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (49, 134, 2418) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7156) [M::mem_pestat] mean and std.dev: (1244.60, 2044.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9525) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (717, 1040, 1346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2604) [M::mem_pestat] mean and std.dev: (1008.53, 527.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3233) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 896.571 CPU sec, 39.680 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (79, 666477, 30, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (50, 83, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 389) [M::mem_pestat] mean and std.dev: (92.06, 64.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 502) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.57, 75.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 100, 408) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1174) [M::mem_pestat] mean and std.dev: (106.46, 177.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1557) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (635, 790, 1029) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1817) [M::mem_pestat] mean and std.dev: (642.89, 304.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2211) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 909.533 CPU sec, 52.180 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (64, 666905, 21, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (37, 80, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 511) [M::mem_pestat] mean and std.dev: (104.31, 99.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 669) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.43, 75.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 76, 142) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 368) [M::mem_pestat] mean and std.dev: (65.65, 45.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 481) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (907, 1320, 2058) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4360) [M::mem_pestat] mean and std.dev: (1406.92, 744.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5511) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 901.929 CPU sec, 40.009 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (84, 666369, 29, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (47, 89, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 431) [M::mem_pestat] mean and std.dev: (98.10, 77.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 559) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.34, 75.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (42, 131, 2488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7380) [M::mem_pestat] mean and std.dev: (833.41, 1380.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9826) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (631, 824, 1409) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2965) [M::mem_pestat] mean and std.dev: (1022.15, 670.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3743) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 906.526 CPU sec, 51.477 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (71, 666801, 33, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (39, 60, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 525) [M::mem_pestat] mean and std.dev: (99.09, 98.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 687) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.31, 75.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (45, 81, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 459) [M::mem_pestat] mean and std.dev: (70.12, 49.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1171, 1520, 1862) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3244) [M::mem_pestat] mean and std.dev: (1307.33, 378.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3935) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 899.429 CPU sec, 39.858 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (70, 666409, 31, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (37, 74, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 427) [M::mem_pestat] mean and std.dev: (97.44, 88.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 557) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.39, 75.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 124, 1643) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4863) [M::mem_pestat] mean and std.dev: (880.27, 1426.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6586) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (455, 815, 1317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3041) [M::mem_pestat] mean and std.dev: (939.64, 666.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3903) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 910.867 CPU sec, 52.614 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (69, 667168, 35, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (35, 73, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 545) [M::mem_pestat] mean and std.dev: (106.84, 95.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 715) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.37, 75.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 100, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (79.11, 65.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 605) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (924, 997, 1382) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (8, 2298) [M::mem_pestat] mean and std.dev: (961.30, 526.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3066) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 903.836 CPU sec, 40.000 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (82, 667273, 30, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (38, 73, 150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 374) [M::mem_pestat] mean and std.dev: (82.19, 71.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 486) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.46, 75.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 136, 456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1314) [M::mem_pestat] mean and std.dev: (151.40, 168.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1743) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 902.852 CPU sec, 47.042 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (81, 666093, 30, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (41, 79, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (97.42, 77.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 533) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.49, 75.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 98, 1704) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5074) [M::mem_pestat] mean and std.dev: (880.11, 1443.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6759) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (454, 756, 1365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3187) [M::mem_pestat] mean and std.dev: (756.82, 512.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4098) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 916.532 CPU sec, 54.239 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (79, 667176, 29, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (40, 70, 147) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 361) [M::mem_pestat] mean and std.dev: (86.77, 62.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 468) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.48, 75.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 114, 1195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3519) [M::mem_pestat] mean and std.dev: (449.26, 851.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4681) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (964, 1155, 1237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (418, 1783) [M::mem_pestat] mean and std.dev: (1101.43, 123.33) [M::mem_pestat] low and high boundaries for proper pairs: (145, 2056) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 909.195 CPU sec, 40.497 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (82, 667200, 30, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (39, 66, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585) [M::mem_pestat] mean and std.dev: (113.46, 133.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 767) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.35, 75.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 143, 1646) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4874) [M::mem_pestat] mean and std.dev: (946.38, 1615.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7407) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 909.698 CPU sec, 53.765 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (78, 666822, 33, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (39, 68, 126) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 300) [M::mem_pestat] mean and std.dev: (77.99, 57.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 387) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.48, 75.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (43, 82, 2252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6670) [M::mem_pestat] mean and std.dev: (800.06, 1381.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8879) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (548, 677, 1230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2594) [M::mem_pestat] mean and std.dev: (746.55, 526.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3276) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 905.463 CPU sec, 40.101 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (68, 666985, 21, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (41, 75, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 584) [M::mem_pestat] mean and std.dev: (101.73, 96.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 765) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.35, 75.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 80, 2020) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6000) [M::mem_pestat] mean and std.dev: (873.50, 1369.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7990) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (854, 1368, 2361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5375) [M::mem_pestat] mean and std.dev: (1334.60, 717.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6882) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 910.500 CPU sec, 53.382 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (77, 667431, 27, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (35, 53, 149) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 377) [M::mem_pestat] mean and std.dev: (78.32, 60.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 491) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.41, 75.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (45, 126, 968) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2814) [M::mem_pestat] mean and std.dev: (255.50, 406.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3737) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 903.241 CPU sec, 40.141 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (83, 666577, 20, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (39, 63, 148) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 366) [M::mem_pestat] mean and std.dev: (87.34, 69.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 475) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.57, 75.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (38, 148, 2478) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7358) [M::mem_pestat] mean and std.dev: (1010.45, 1501.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9798) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (838, 1664, 2998) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7318) [M::mem_pestat] mean and std.dev: (1551.67, 994.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9478) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 915.014 CPU sec, 53.441 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (92, 667231, 23, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (43, 65, 147) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 355) [M::mem_pestat] mean and std.dev: (84.86, 66.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 459) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.58, 75.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (43, 74, 582) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1660) [M::mem_pestat] mean and std.dev: (254.40, 466.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2199) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (936, 1306, 2170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4638) [M::mem_pestat] mean and std.dev: (1214.27, 629.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5872) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 903.972 CPU sec, 40.323 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (71, 667311, 33, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (42, 85, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 429) [M::mem_pestat] mean and std.dev: (82.10, 58.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 558) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.36, 75.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (39, 128, 1584) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4674) [M::mem_pestat] mean and std.dev: (811.59, 1310.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6219) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (626, 801, 2133) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5147) [M::mem_pestat] mean and std.dev: (1201.69, 894.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6654) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 912.264 CPU sec, 53.392 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (85, 666646, 32, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (40, 64, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (92.67, 81.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 660) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.40, 75.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (45, 89, 458) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1284) [M::mem_pestat] mean and std.dev: (90.96, 101.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1697) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (592, 707, 1399) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3013) [M::mem_pestat] mean and std.dev: (919.42, 709.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3820) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 911.234 CPU sec, 40.528 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (79, 666732, 25, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (38, 69, 149) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 371) [M::mem_pestat] mean and std.dev: (87.19, 69.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 482) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.50, 75.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 117, 464) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1350) [M::mem_pestat] mean and std.dev: (175.09, 258.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1793) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 916.848 CPU sec, 54.430 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (81, 666886, 34, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (37, 61, 128) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 310) [M::mem_pestat] mean and std.dev: (74.18, 50.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 401) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.36, 75.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 126, 454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1294) [M::mem_pestat] mean and std.dev: (129.93, 146.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1714) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (542, 957, 1884) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4568) [M::mem_pestat] mean and std.dev: (1003.15, 748.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5910) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 908.818 CPU sec, 40.614 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (69, 666894, 30, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (42, 56, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492) [M::mem_pestat] mean and std.dev: (87.35, 72.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 642) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.38, 75.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 48, 97) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 247) [M::mem_pestat] mean and std.dev: (44.52, 30.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 322) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 910.523 CPU sec, 53.951 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (86, 667076, 37, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (41, 70, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (91.64, 76.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 533) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.45, 75.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 93, 2026) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6034) [M::mem_pestat] mean and std.dev: (935.11, 1662.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8038) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 905.964 CPU sec, 40.262 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (74, 666741, 30, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (38, 68, 152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 380) [M::mem_pestat] mean and std.dev: (74.25, 56.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 494) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.33, 75.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 64, 155) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 399) [M::mem_pestat] mean and std.dev: (90.18, 85.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 521) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1115, 1524, 2945) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6605) [M::mem_pestat] mean and std.dev: (1904.60, 1287.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8435) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 903.072 CPU sec, 44.715 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (59, 666861, 21, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (38, 49, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 404) [M::mem_pestat] mean and std.dev: (79.76, 68.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 526) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (11, 521) [M::mem_pestat] mean and std.dev: (269.17, 75.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 98, 4260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12714) [M::mem_pestat] mean and std.dev: (1783.43, 2447.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16941) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (405, 851, 2044) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5322) [M::mem_pestat] mean and std.dev: (1030.47, 760.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6961) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 913.186 CPU sec, 54.018 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (62, 667588, 35, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (37, 67, 123) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 295) [M::mem_pestat] mean and std.dev: (76.18, 54.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 381) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.46, 75.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (65, 131, 448) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1214) [M::mem_pestat] mean and std.dev: (235.58, 334.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1597) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (433, 863, 1296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3022) [M::mem_pestat] mean and std.dev: (821.88, 612.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3885) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 899.399 CPU sec, 39.977 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (78, 666708, 24, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (41, 63, 150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 368) [M::mem_pestat] mean and std.dev: (86.94, 72.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 477) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.28, 75.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (63, 152, 553) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1533) [M::mem_pestat] mean and std.dev: (192.50, 199.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2023) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (743, 1299, 1491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2987) [M::mem_pestat] mean and std.dev: (916.67, 500.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3735) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 910.981 CPU sec, 53.235 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (71, 666625, 30, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (39, 53, 134) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 324) [M::mem_pestat] mean and std.dev: (77.09, 59.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 419) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 262, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (11, 521) [M::mem_pestat] mean and std.dev: (269.04, 75.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 114, 1687) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4993) [M::mem_pestat] mean and std.dev: (813.71, 1287.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6646) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (452, 934, 1376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3224) [M::mem_pestat] mean and std.dev: (929.61, 629.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4148) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 908.139 CPU sec, 40.399 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (84, 666726, 38, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (43, 88, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 517) [M::mem_pestat] mean and std.dev: (104.95, 93.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 675) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.52, 75.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 92, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 555) [M::mem_pestat] mean and std.dev: (75.70, 59.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 729) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (404, 775, 1431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3485) [M::mem_pestat] mean and std.dev: (727.60, 439.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4512) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 916.640 CPU sec, 54.224 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (72, 667654, 27, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (47, 74, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 449) [M::mem_pestat] mean and std.dev: (90.35, 69.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 583) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.42, 75.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (38, 102, 2731) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8117) [M::mem_pestat] mean and std.dev: (1182.48, 1787.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10810) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (146, 611, 1087) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2969) [M::mem_pestat] mean and std.dev: (710.00, 681.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3910) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 908.383 CPU sec, 40.406 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (70, 666673, 24, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (37, 77, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637) [M::mem_pestat] mean and std.dev: (103.71, 120.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 837) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.45, 75.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 58, 117) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 303) [M::mem_pestat] mean and std.dev: (56.95, 47.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 396) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (747, 1261, 2004) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4518) [M::mem_pestat] mean and std.dev: (1419.00, 1103.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5831) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 917.853 CPU sec, 54.876 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (66, 667381, 26, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (34, 64, 153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 391) [M::mem_pestat] mean and std.dev: (71.47, 51.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.28, 75.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 102, 662) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1928) [M::mem_pestat] mean and std.dev: (105.70, 147.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2561) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (768, 1094, 1458) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2838) [M::mem_pestat] mean and std.dev: (1029.92, 421.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3528) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 909.618 CPU sec, 40.493 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (71, 666184, 18, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (39, 85, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591) [M::mem_pestat] mean and std.dev: (120.55, 129.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 775) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.27, 75.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 44, 129) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 321) [M::mem_pestat] mean and std.dev: (49.29, 37.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 417) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (237, 704, 1455) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3891) [M::mem_pestat] mean and std.dev: (623.18, 477.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5109) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 917.366 CPU sec, 54.243 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (77, 667253, 25, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (40, 79, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (106.14, 86.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 616) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.53, 75.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 45, 924) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2710) [M::mem_pestat] mean and std.dev: (471.54, 832.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3800) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (514, 646, 1109) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2299) [M::mem_pestat] mean and std.dev: (734.33, 552.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2946) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 903.584 CPU sec, 40.230 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (62, 666773, 30, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 96, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 439) [M::mem_pestat] mean and std.dev: (105.58, 73.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 567) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.43, 75.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 71, 134) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 338) [M::mem_pestat] mean and std.dev: (64.92, 45.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 440) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (760, 851, 1163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1969) [M::mem_pestat] mean and std.dev: (801.40, 351.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2372) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 915.640 CPU sec, 54.006 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (59, 666229, 28, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (39, 68, 131) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 315) [M::mem_pestat] mean and std.dev: (77.40, 55.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 407) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.33, 75.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (42, 113, 533) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1515) [M::mem_pestat] mean and std.dev: (111.59, 132.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2006) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1031, 1218, 1634) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2840) [M::mem_pestat] mean and std.dev: (1231.30, 337.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3443) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 917.545 CPU sec, 40.851 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (76, 667122, 20, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (35, 59, 106) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 248) [M::mem_pestat] mean and std.dev: (69.55, 45.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 319) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.33, 75.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (68, 179, 1553) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4523) [M::mem_pestat] mean and std.dev: (493.82, 630.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6008) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (772, 985, 1654) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3418) [M::mem_pestat] mean and std.dev: (1186.93, 729.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4300) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 909.941 CPU sec, 52.522 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (72, 667094, 36, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (40, 83, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 448) [M::mem_pestat] mean and std.dev: (97.56, 77.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 584) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.47, 75.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 121, 3009) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8959) [M::mem_pestat] mean and std.dev: (1457.36, 2301.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11934) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (561, 1394, 1453) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3237) [M::mem_pestat] mean and std.dev: (951.56, 476.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4129) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 912.148 CPU sec, 40.510 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (69, 666751, 25, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (39, 81, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492) [M::mem_pestat] mean and std.dev: (99.23, 84.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 643) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.50, 75.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (41, 136, 2580) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7658) [M::mem_pestat] mean and std.dev: (1029.08, 1519.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10197) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (582, 1526, 2352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5892) [M::mem_pestat] mean and std.dev: (1560.88, 1281.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7662) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 900.882 CPU sec, 41.738 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (67, 666474, 22, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (34, 80, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 442) [M::mem_pestat] mean and std.dev: (94.57, 72.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.28, 75.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (41, 123, 2974) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8840) [M::mem_pestat] mean and std.dev: (1542.05, 2199.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11773) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 913.987 CPU sec, 54.534 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (80, 666334, 25, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (39, 64, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (95.80, 88.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.42, 75.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (40, 86, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 976) [M::mem_pestat] mean and std.dev: (89.25, 77.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1288) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (882, 1944, 2984) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7188) [M::mem_pestat] mean and std.dev: (2010.12, 1364.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9290) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 914.596 CPU sec, 40.508 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (69, 666745, 35, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (35, 59, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 419) [M::mem_pestat] mean and std.dev: (80.36, 63.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 547) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.31, 75.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (43, 93, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 538) [M::mem_pestat] mean and std.dev: (70.89, 53.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 703) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (897, 1349, 3233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7905) [M::mem_pestat] mean and std.dev: (2107.83, 1985.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10241) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 912.282 CPU sec, 54.434 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (78, 666571, 18, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (42, 95, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (100.37, 90.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 674) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.26, 75.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 127, 1016) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3002) [M::mem_pestat] mean and std.dev: (294.80, 613.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3995) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (399, 690, 1172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2718) [M::mem_pestat] mean and std.dev: (672.17, 373.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3491) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 908.024 CPU sec, 40.502 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (75, 666970, 27, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (37, 80, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 457) [M::mem_pestat] mean and std.dev: (101.55, 87.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.52, 75.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 115, 1948) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5794) [M::mem_pestat] mean and std.dev: (854.54, 1286.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7717) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (709, 904, 1793) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3961) [M::mem_pestat] mean and std.dev: (1010.18, 799.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5045) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 910.849 CPU sec, 53.331 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (87, 667074, 30, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (39, 73, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 555) [M::mem_pestat] mean and std.dev: (90.42, 82.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 727) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 262, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.17, 75.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (61, 155, 2506) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7396) [M::mem_pestat] mean and std.dev: (1184.00, 1669.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9841) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (803, 903, 1616) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3242) [M::mem_pestat] mean and std.dev: (972.11, 409.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4055) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 912.244 CPU sec, 40.444 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (75, 667271, 25, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (43, 65, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424) [M::mem_pestat] mean and std.dev: (93.90, 82.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 551) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.46, 75.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 108, 3619) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10809) [M::mem_pestat] mean and std.dev: (1352.40, 2387.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14404) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1002, 1191, 2387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5157) [M::mem_pestat] mean and std.dev: (1386.23, 890.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6542) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 913.830 CPU sec, 53.941 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (79, 667381, 35, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (45, 78, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609) [M::mem_pestat] mean and std.dev: (115.55, 117.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 797) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.49, 75.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 96, 1377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4063) [M::mem_pestat] mean and std.dev: (247.45, 500.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5406) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (730, 1080, 1458) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2914) [M::mem_pestat] mean and std.dev: (1060.69, 493.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3642) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 913.870 CPU sec, 40.758 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (72, 666576, 19, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (45, 78, 131) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 303) [M::mem_pestat] mean and std.dev: (85.96, 54.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 389) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.45, 75.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (46, 78, 1548) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4552) [M::mem_pestat] mean and std.dev: (678.94, 1160.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6054) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (544, 1063, 2439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6229) [M::mem_pestat] mean and std.dev: (1649.56, 1850.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9050) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 910.991 CPU sec, 53.584 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (57, 667086, 34, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (41, 54, 132) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 314) [M::mem_pestat] mean and std.dev: (84.98, 66.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 405) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.42, 75.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 68, 901) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2637) [M::mem_pestat] mean and std.dev: (225.10, 402.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3505) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 906.837 CPU sec, 40.360 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (71, 666712, 31, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (40, 58, 148) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 364) [M::mem_pestat] mean and std.dev: (87.08, 66.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 472) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.46, 75.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (59, 144, 1623) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4751) [M::mem_pestat] mean and std.dev: (650.54, 1119.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6315) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (494, 824, 1097) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2303) [M::mem_pestat] mean and std.dev: (633.91, 327.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2906) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 911.723 CPU sec, 54.115 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (79, 667321, 24, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (40, 74, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 592) [M::mem_pestat] mean and std.dev: (103.39, 92.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 776) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.44, 75.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 97, 883) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2583) [M::mem_pestat] mean and std.dev: (144.16, 253.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3433) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (628, 1104, 1755) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4009) [M::mem_pestat] mean and std.dev: (1085.33, 832.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5136) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 910.353 CPU sec, 40.614 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (74, 667231, 32, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (42, 77, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402) [M::mem_pestat] mean and std.dev: (81.50, 58.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 522) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.38, 75.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 74, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622) [M::mem_pestat] mean and std.dev: (89.81, 121.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 819) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (589, 782, 1531) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3415) [M::mem_pestat] mean and std.dev: (775.33, 400.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4357) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 910.083 CPU sec, 53.904 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (81, 666584, 22, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (39, 58, 128) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 306) [M::mem_pestat] mean and std.dev: (79.71, 61.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 395) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.50, 75.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (45, 72, 136) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 318) [M::mem_pestat] mean and std.dev: (75.79, 50.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 409) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (596, 879, 1096) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2096) [M::mem_pestat] mean and std.dev: (682.25, 364.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2596) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 907.534 CPU sec, 40.355 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (75, 667553, 34, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (44, 91, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (106.78, 88.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 640) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.35, 75.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 99, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460) [M::mem_pestat] mean and std.dev: (76.85, 56.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (783, 1102, 2020) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4494) [M::mem_pestat] mean and std.dev: (1215.92, 665.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5731) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 906.693 CPU sec, 52.734 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (74, 666474, 32, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (41, 85, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 413) [M::mem_pestat] mean and std.dev: (91.69, 73.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 537) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.50, 75.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 50, 118) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 296) [M::mem_pestat] mean and std.dev: (57.64, 46.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 385) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (685, 849, 1240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2350) [M::mem_pestat] mean and std.dev: (907.76, 498.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2905) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 905.199 CPU sec, 40.267 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (76, 666971, 31, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (40, 77, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 715) [M::mem_pestat] mean and std.dev: (110.33, 119.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 940) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.49, 75.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 103, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1245) [M::mem_pestat] mean and std.dev: (106.50, 120.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1650) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (804, 985, 2296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5280) [M::mem_pestat] mean and std.dev: (1356.00, 1123.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6772) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 916.824 CPU sec, 54.093 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (69, 666663, 24, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (43, 76, 158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388) [M::mem_pestat] mean and std.dev: (95.53, 76.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 503) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.44, 75.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 50, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1023) [M::mem_pestat] mean and std.dev: (98.15, 161.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1356) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (608, 1034, 1341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2807) [M::mem_pestat] mean and std.dev: (810.93, 445.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3540) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 911.669 CPU sec, 40.446 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (81, 667418, 20, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (34, 52, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 457) [M::mem_pestat] mean and std.dev: (86.26, 75.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.33, 75.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (65, 140, 4384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13022) [M::mem_pestat] mean and std.dev: (1913.20, 2935.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17341) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 906.163 CPU sec, 53.538 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (87, 666706, 29, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (46, 70, 159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 385) [M::mem_pestat] mean and std.dev: (79.66, 55.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 498) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.36, 75.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 50, 808) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2364) [M::mem_pestat] mean and std.dev: (140.42, 259.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3142) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (271, 764, 1213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3097) [M::mem_pestat] mean and std.dev: (695.42, 430.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4039) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 908.367 CPU sec, 40.375 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (66, 666669, 37, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (39, 63, 120) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 282) [M::mem_pestat] mean and std.dev: (79.15, 61.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 363) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.36, 75.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (38, 75, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 620) [M::mem_pestat] mean and std.dev: (87.57, 108.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 814) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (772, 1117, 1442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2782) [M::mem_pestat] mean and std.dev: (913.00, 441.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3452) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 914.212 CPU sec, 53.881 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (60, 666388, 23, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (44, 66, 141) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 335) [M::mem_pestat] mean and std.dev: (77.81, 55.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 432) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.29, 75.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (43, 142, 4443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13243) [M::mem_pestat] mean and std.dev: (1704.91, 2511.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17643) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 910.491 CPU sec, 40.453 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (64, 667219, 30, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (38, 73, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458) [M::mem_pestat] mean and std.dev: (91.29, 69.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.33, 75.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (48, 115, 1447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4245) [M::mem_pestat] mean and std.dev: (478.30, 912.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5644) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (734, 1093, 2166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5030) [M::mem_pestat] mean and std.dev: (1441.69, 1198.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6462) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 912.792 CPU sec, 54.275 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (73, 666584, 24, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (43, 71, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403) [M::mem_pestat] mean and std.dev: (102.28, 84.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 523) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.42, 75.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 60, 402) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1142) [M::mem_pestat] mean and std.dev: (65.42, 85.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1512) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (737, 1415, 1849) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4073) [M::mem_pestat] mean and std.dev: (1287.54, 770.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5185) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 909.688 CPU sec, 40.464 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (71, 666964, 27, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (44, 87, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 536) [M::mem_pestat] mean and std.dev: (90.74, 70.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 700) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.40, 75.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 69, 1268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3734) [M::mem_pestat] mean and std.dev: (657.81, 1107.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5088) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (745, 1490, 2004) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4522) [M::mem_pestat] mean and std.dev: (1296.54, 745.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5781) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 915.827 CPU sec, 55.204 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (73, 666750, 32, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (44, 59, 135) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 317) [M::mem_pestat] mean and std.dev: (77.10, 55.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 408) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.37, 75.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (38, 122, 1337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3935) [M::mem_pestat] mean and std.dev: (515.25, 846.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5234) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (705, 891, 1390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2760) [M::mem_pestat] mean and std.dev: (906.15, 562.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3445) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 914.624 CPU sec, 40.749 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (82, 666449, 29, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (43, 82, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 439) [M::mem_pestat] mean and std.dev: (106.66, 86.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 571) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.31, 75.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (50, 90, 700) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2000) [M::mem_pestat] mean and std.dev: (167.92, 270.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2650) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (674, 696, 777) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (468, 983) [M::mem_pestat] mean and std.dev: (686.71, 59.67) [M::mem_pestat] low and high boundaries for proper pairs: (365, 1086) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 912.359 CPU sec, 52.761 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (76, 666432, 31, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (48, 74, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492) [M::mem_pestat] mean and std.dev: (88.58, 73.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 640) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.47, 75.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (52, 127, 747) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2137) [M::mem_pestat] mean and std.dev: (236.38, 403.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2832) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (905, 1119, 1897) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3881) [M::mem_pestat] mean and std.dev: (1140.77, 698.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4873) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 908.490 CPU sec, 40.461 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (76, 667040, 32, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (41, 59, 130) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 308) [M::mem_pestat] mean and std.dev: (67.98, 45.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 397) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.40, 75.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (42, 91, 1596) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4704) [M::mem_pestat] mean and std.dev: (599.48, 1188.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6258) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 910.651 CPU sec, 54.805 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (75, 667043, 26, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (41, 89, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (88.98, 66.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 669) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.39, 75.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 147, 1685) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4991) [M::mem_pestat] mean and std.dev: (757.96, 1067.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6644) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (506, 1455, 2916) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7736) [M::mem_pestat] mean and std.dev: (1491.78, 1574.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10146) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 912.900 CPU sec, 53.889 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (75, 667001, 25, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (43, 58, 148) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 358) [M::mem_pestat] mean and std.dev: (91.20, 78.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 463) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.49, 75.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (45, 100, 406) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1128) [M::mem_pestat] mean and std.dev: (144.00, 229.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1489) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (695, 886, 1701) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3713) [M::mem_pestat] mean and std.dev: (817.50, 434.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4719) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 908.221 CPU sec, 40.339 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (77, 666885, 28, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (39, 64, 140) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 342) [M::mem_pestat] mean and std.dev: (89.46, 74.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 443) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.38, 75.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (42, 67, 892) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2592) [M::mem_pestat] mean and std.dev: (243.00, 457.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3442) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 911.757 CPU sec, 53.946 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (68, 667159, 26, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (45, 64, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420) [M::mem_pestat] mean and std.dev: (95.54, 83.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 545) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.38, 75.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 55, 88) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 212) [M::mem_pestat] mean and std.dev: (50.73, 41.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 274) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (888, 1057, 1779) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3561) [M::mem_pestat] mean and std.dev: (1148.40, 646.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4452) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 909.934 CPU sec, 40.349 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (78, 667031, 27, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (39, 66, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (90.97, 75.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 527) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.58, 75.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (38, 134, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695) [M::mem_pestat] mean and std.dev: (127.52, 147.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 914) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (236, 711, 1582) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4274) [M::mem_pestat] mean and std.dev: (827.92, 650.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5620) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 911.636 CPU sec, 54.323 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (71, 667024, 27, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (48, 66, 140) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 324) [M::mem_pestat] mean and std.dev: (84.23, 57.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 416) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 262, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (11, 521) [M::mem_pestat] mean and std.dev: (269.15, 75.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 86, 3486) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10384) [M::mem_pestat] mean and std.dev: (1304.00, 1885.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13833) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (754, 1094, 1253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2251) [M::mem_pestat] mean and std.dev: (991.35, 492.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2961) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 904.883 CPU sec, 40.238 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (71, 666353, 29, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (39, 68, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (84.42, 66.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 531) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.40, 75.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 48, 142) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 386) [M::mem_pestat] mean and std.dev: (53.48, 46.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 508) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (211, 846, 2616) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7426) [M::mem_pestat] mean and std.dev: (1559.29, 1714.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9831) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 912.485 CPU sec, 52.558 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (75, 666918, 18, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (41, 64, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 437) [M::mem_pestat] mean and std.dev: (102.41, 92.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 569) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.49, 75.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 69, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 448) [M::mem_pestat] mean and std.dev: (69.50, 52.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 588) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (467, 801, 2032) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5162) [M::mem_pestat] mean and std.dev: (996.38, 832.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6727) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 907.056 CPU sec, 40.309 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (65, 666459, 31, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (43, 84, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (94.05, 71.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 531) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.42, 75.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (39, 80, 534) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1524) [M::mem_pestat] mean and std.dev: (143.08, 250.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2019) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (876, 1239, 1740) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3468) [M::mem_pestat] mean and std.dev: (1342.05, 768.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4417) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 910.744 CPU sec, 53.175 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (64, 667657, 25, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (41, 65, 144) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 350) [M::mem_pestat] mean and std.dev: (82.96, 69.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 453) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.33, 75.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 90, 517) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1503) [M::mem_pestat] mean and std.dev: (179.09, 247.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1996) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 909.840 CPU sec, 40.390 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (83, 667481, 28, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (40, 79, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (90.00, 80.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 644) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.37, 75.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (48, 66, 122) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 270) [M::mem_pestat] mean and std.dev: (59.61, 36.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 344) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (660, 849, 1862) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4266) [M::mem_pestat] mean and std.dev: (1032.00, 808.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5468) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 912.892 CPU sec, 53.525 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (66, 667244, 34, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (41, 110, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 515) [M::mem_pestat] mean and std.dev: (101.81, 71.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 673) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.35, 75.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 115, 1245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3669) [M::mem_pestat] mean and std.dev: (604.53, 1060.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4881) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 908.293 CPU sec, 40.267 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (61, 666695, 32, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (41, 61, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 524) [M::mem_pestat] mean and std.dev: (106.93, 106.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 685) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.38, 75.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (51, 105, 785) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2253) [M::mem_pestat] mean and std.dev: (279.45, 464.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2987) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (279, 708, 1549) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4089) [M::mem_pestat] mean and std.dev: (845.00, 670.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5359) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 913.944 CPU sec, 54.604 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (80, 666224, 22, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (37, 62, 115) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 271) [M::mem_pestat] mean and std.dev: (72.60, 47.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 349) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.38, 75.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (60, 177, 1291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3753) [M::mem_pestat] mean and std.dev: (550.20, 825.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4984) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 912.359 CPU sec, 40.484 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (78, 666293, 24, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (43, 72, 149) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 361) [M::mem_pestat] mean and std.dev: (80.54, 55.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 467) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.35, 75.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (79, 187, 2543) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7471) [M::mem_pestat] mean and std.dev: (1139.13, 1697.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9935) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (806, 1513, 3975) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10313) [M::mem_pestat] mean and std.dev: (2025.42, 1740.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13482) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 916.938 CPU sec, 54.305 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (75, 667081, 35, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (49, 88, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (103.33, 86.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 621) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.54, 75.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (47, 120, 1630) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4796) [M::mem_pestat] mean and std.dev: (786.88, 1259.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6379) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (311, 929, 1234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3080) [M::mem_pestat] mean and std.dev: (875.78, 580.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4003) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 915.722 CPU sec, 55.304 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (70, 667716, 31, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (41, 103, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 545) [M::mem_pestat] mean and std.dev: (115.50, 91.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 713) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.33, 75.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 88, 2835) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8439) [M::mem_pestat] mean and std.dev: (1082.50, 1746.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11241) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (809, 1066, 1436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2690) [M::mem_pestat] mean and std.dev: (1106.11, 623.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3598) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 907.689 CPU sec, 40.472 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (73, 667226, 27, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (33, 54, 105) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 249) [M::mem_pestat] mean and std.dev: (65.26, 48.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 321) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.43, 75.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (48, 109, 1664) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4896) [M::mem_pestat] mean and std.dev: (820.33, 1192.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6512) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1119, 1611, 2635) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5667) [M::mem_pestat] mean and std.dev: (1610.42, 817.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7183) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 914.836 CPU sec, 54.647 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (65, 667064, 25, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (39, 73, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (105.13, 94.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.34, 75.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (53, 106, 416) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1142) [M::mem_pestat] mean and std.dev: (110.20, 96.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1505) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (546, 1151, 1802) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4314) [M::mem_pestat] mean and std.dev: (1200.06, 757.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5570) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 908.109 CPU sec, 40.235 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (76, 666909, 26, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (43, 72, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 394) [M::mem_pestat] mean and std.dev: (85.23, 66.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 511) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.43, 75.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (53, 105, 153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 353) [M::mem_pestat] mean and std.dev: (78.71, 44.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 453) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (775, 1043, 1502) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2956) [M::mem_pestat] mean and std.dev: (1029.46, 525.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3683) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 919.012 CPU sec, 54.481 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (61, 667810, 34, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (39, 63, 126) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 300) [M::mem_pestat] mean and std.dev: (70.80, 47.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 387) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.57, 75.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 83, 2043) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6089) [M::mem_pestat] mean and std.dev: (984.18, 1527.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8112) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (782, 896, 1439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2753) [M::mem_pestat] mean and std.dev: (792.36, 430.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3410) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 903.148 CPU sec, 40.057 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (65, 666678, 31, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (40, 92, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 607) [M::mem_pestat] mean and std.dev: (122.43, 106.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 796) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.46, 75.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (60, 127, 2623) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7749) [M::mem_pestat] mean and std.dev: (1364.32, 1877.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10312) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (730, 962, 1463) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2929) [M::mem_pestat] mean and std.dev: (888.67, 424.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3662) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 905.126 CPU sec, 52.646 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (86, 667173, 34, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (47, 77, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (97.47, 74.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 667) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.35, 75.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (46, 130, 3206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9526) [M::mem_pestat] mean and std.dev: (1697.24, 2752.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12707) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 909.980 CPU sec, 40.565 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (71, 666549, 40, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (47, 87, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 572) [M::mem_pestat] mean and std.dev: (104.70, 82.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 747) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.39, 75.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 70, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 832) [M::mem_pestat] mean and std.dev: (99.40, 124.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1101) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (559, 745, 1106) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2200) [M::mem_pestat] mean and std.dev: (896.27, 612.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3345) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 914.051 CPU sec, 53.432 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (71, 666946, 28, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (49, 86, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (97.95, 68.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 545) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (216, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (12, 522) [M::mem_pestat] mean and std.dev: (269.56, 75.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 624) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 91, 2908) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8652) [M::mem_pestat] mean and std.dev: (1023.04, 1697.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11524) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (477, 878, 1331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3039) [M::mem_pestat] mean and std.dev: (903.93, 603.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3893) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 906.890 CPU sec, 40.404 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (70, 666918, 18, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (38, 57, 140) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 344) [M::mem_pestat] mean and std.dev: (84.23, 68.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 446) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (216, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (12, 522) [M::mem_pestat] mean and std.dev: (269.69, 75.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 624) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 61, 92) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 220) [M::mem_pestat] mean and std.dev: (58.25, 50.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 284) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (788, 976, 2149) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4871) [M::mem_pestat] mean and std.dev: (1367.00, 1047.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6232) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 914.328 CPU sec, 53.910 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (65, 666552, 31, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (44, 84, 157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 383) [M::mem_pestat] mean and std.dev: (93.98, 68.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 496) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.57, 75.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 43, 106) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 262) [M::mem_pestat] mean and std.dev: (46.38, 35.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 340) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 908.211 CPU sec, 40.410 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (59, 666895, 23, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (46, 83, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 433) [M::mem_pestat] mean and std.dev: (109.85, 93.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 562) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.41, 75.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 79, 3331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9929) [M::mem_pestat] mean and std.dev: (1617.65, 2670.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13228) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (822, 1128, 1531) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2949) [M::mem_pestat] mean and std.dev: (1130.00, 575.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3658) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 915.226 CPU sec, 54.366 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (78, 666909, 27, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (42, 68, 145) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 351) [M::mem_pestat] mean and std.dev: (88.32, 65.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 454) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.35, 75.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 88, 158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (69.59, 56.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 533) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (654, 814, 955) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (52, 1557) [M::mem_pestat] mean and std.dev: (748.23, 336.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2093) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 908.665 CPU sec, 40.388 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (80, 666496, 27, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (35, 65, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 530) [M::mem_pestat] mean and std.dev: (92.24, 91.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 695) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.34, 75.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (38, 88, 1113) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3263) [M::mem_pestat] mean and std.dev: (273.83, 496.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4338) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (886, 1164, 1366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2326) [M::mem_pestat] mean and std.dev: (1006.75, 467.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2877) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 912.045 CPU sec, 53.760 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (67, 666377, 29, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (48, 94, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 579) [M::mem_pestat] mean and std.dev: (108.52, 97.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 756) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.38, 75.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (38, 128, 1850) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5474) [M::mem_pestat] mean and std.dev: (713.68, 1154.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7286) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (498, 998, 2145) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5439) [M::mem_pestat] mean and std.dev: (1094.55, 829.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7086) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 916.198 CPU sec, 52.537 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (77, 666641, 37, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (35, 74, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 485) [M::mem_pestat] mean and std.dev: (99.21, 79.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.46, 75.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (41, 135, 2959) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8795) [M::mem_pestat] mean and std.dev: (1155.35, 1651.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11713) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (528, 961, 1679) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3981) [M::mem_pestat] mean and std.dev: (1049.59, 736.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5132) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 911.776 CPU sec, 40.156 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (66, 666814, 37, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 91, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (108.24, 79.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 664) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (216, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (12, 522) [M::mem_pestat] mean and std.dev: (269.47, 75.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 624) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 63, 1038) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3052) [M::mem_pestat] mean and std.dev: (349.88, 789.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4059) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (627, 816, 1709) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3873) [M::mem_pestat] mean and std.dev: (947.58, 560.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4955) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 906.033 CPU sec, 52.902 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (72, 666452, 33, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (47, 98, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 422) [M::mem_pestat] mean and std.dev: (106.58, 76.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 547) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (216, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (12, 522) [M::mem_pestat] mean and std.dev: (269.49, 75.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 624) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (41, 165, 1566) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4616) [M::mem_pestat] mean and std.dev: (715.35, 1276.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6141) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 905.552 CPU sec, 40.212 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (89, 666827, 30, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (36, 81, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 456) [M::mem_pestat] mean and std.dev: (97.35, 81.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 596) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.29, 75.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 73, 498) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1444) [M::mem_pestat] mean and std.dev: (138.12, 209.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1917) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (519, 776, 1374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3084) [M::mem_pestat] mean and std.dev: (611.27, 380.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3939) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 913.020 CPU sec, 53.838 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (67, 666892, 24, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (44, 65, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 413) [M::mem_pestat] mean and std.dev: (93.79, 70.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 536) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.57, 75.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (42, 132, 816) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2364) [M::mem_pestat] mean and std.dev: (141.53, 202.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3138) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 907.171 CPU sec, 40.261 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (69, 667274, 30, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (43, 82, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 436) [M::mem_pestat] mean and std.dev: (93.79, 72.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 567) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.44, 75.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (90, 160, 674) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1842) [M::mem_pestat] mean and std.dev: (285.62, 356.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2426) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (656, 1133, 1509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3215) [M::mem_pestat] mean and std.dev: (1179.86, 599.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4068) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 910.498 CPU sec, 53.027 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (77, 667216, 39, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (34, 72, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (87.93, 67.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 546) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (11, 521) [M::mem_pestat] mean and std.dev: (269.17, 75.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (43, 69, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757) [M::mem_pestat] mean and std.dev: (94.52, 117.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 995) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (637, 954, 1469) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3133) [M::mem_pestat] mean and std.dev: (894.53, 468.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3965) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 909.461 CPU sec, 40.460 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (88, 667601, 34, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (42, 76, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 549) [M::mem_pestat] mean and std.dev: (100.57, 90.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 718) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.50, 75.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 55, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 422) [M::mem_pestat] mean and std.dev: (52.31, 38.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 551) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (566, 791, 1347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2909) [M::mem_pestat] mean and std.dev: (916.07, 522.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3690) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 904.428 CPU sec, 53.288 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (85, 666539, 26, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (46, 95, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 520) [M::mem_pestat] mean and std.dev: (103.35, 88.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 678) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (216, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (12, 522) [M::mem_pestat] mean and std.dev: (269.41, 75.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 624) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (63, 994, 4074) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12096) [M::mem_pestat] mean and std.dev: (1811.00, 2208.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16107) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (787, 960, 1476) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2854) [M::mem_pestat] mean and std.dev: (944.90, 611.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3543) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 907.827 CPU sec, 40.421 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (79, 667637, 28, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (44, 70, 155) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 377) [M::mem_pestat] mean and std.dev: (85.06, 62.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 488) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.54, 75.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (60, 125, 391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1053) [M::mem_pestat] mean and std.dev: (157.92, 199.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1384) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (212, 1405, 1986) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5534) [M::mem_pestat] mean and std.dev: (1488.80, 1281.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7308) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 908.732 CPU sec, 52.625 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (81, 666347, 35, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (41, 74, 135) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 323) [M::mem_pestat] mean and std.dev: (85.81, 59.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 417) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.42, 75.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (41, 103, 1979) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5855) [M::mem_pestat] mean and std.dev: (693.76, 1259.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7793) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (809, 1032, 2115) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4727) [M::mem_pestat] mean and std.dev: (1362.31, 1141.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6033) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 913.948 CPU sec, 40.707 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (66, 666615, 25, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (47, 96, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 626) [M::mem_pestat] mean and std.dev: (112.89, 110.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 819) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.48, 75.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 56, 147) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (54.90, 50.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 528) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (565, 706, 1159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2347) [M::mem_pestat] mean and std.dev: (681.05, 388.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2941) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 911.584 CPU sec, 54.080 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (83, 667016, 34, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (41, 80, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 596) [M::mem_pestat] mean and std.dev: (94.96, 100.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 781) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.44, 75.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 65, 126) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 312) [M::mem_pestat] mean and std.dev: (56.19, 36.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 405) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (216, 828, 1318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3522) [M::mem_pestat] mean and std.dev: (858.92, 706.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4624) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 912.565 CPU sec, 40.554 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (74, 667257, 37, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (43, 82, 159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 391) [M::mem_pestat] mean and std.dev: (93.69, 68.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 507) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.42, 75.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 81, 1212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3578) [M::mem_pestat] mean and std.dev: (245.26, 550.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4761) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (176, 913, 1187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3209) [M::mem_pestat] mean and std.dev: (695.85, 517.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4220) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 910.680 CPU sec, 52.095 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (90, 665807, 34, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (39, 58, 142) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 348) [M::mem_pestat] mean and std.dev: (79.18, 69.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 451) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.45, 75.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 84, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 441) [M::mem_pestat] mean and std.dev: (77.38, 69.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 580) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (266, 727, 2098) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5762) [M::mem_pestat] mean and std.dev: (1254.30, 1243.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7594) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 920.791 CPU sec, 56.004 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (69, 666645, 29, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (46, 66, 121) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 271) [M::mem_pestat] mean and std.dev: (77.38, 53.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 346) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.40, 75.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (41, 69, 150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 368) [M::mem_pestat] mean and std.dev: (76.73, 61.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 477) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (944, 2286, 2596) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5900) [M::mem_pestat] mean and std.dev: (1923.60, 974.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7552) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 910.589 CPU sec, 40.489 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (84, 667007, 27, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (37, 68, 141) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 349) [M::mem_pestat] mean and std.dev: (83.25, 64.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 453) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.45, 75.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (47, 163, 1765) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5201) [M::mem_pestat] mean and std.dev: (1064.37, 1598.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7458) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (658, 861, 1485) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3139) [M::mem_pestat] mean and std.dev: (843.60, 475.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3966) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 911.894 CPU sec, 54.116 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 666466, 31, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (48, 93, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 708) [M::mem_pestat] mean and std.dev: (137.00, 135.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 928) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (11, 521) [M::mem_pestat] mean and std.dev: (269.21, 75.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (43, 88, 385) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1069) [M::mem_pestat] mean and std.dev: (108.68, 129.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1411) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 912.747 CPU sec, 40.582 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (67, 666596, 34, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (37, 55, 96) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 214) [M::mem_pestat] mean and std.dev: (68.69, 43.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 273) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.42, 75.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (58, 141, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 799) [M::mem_pestat] mean and std.dev: (111.12, 74.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1046) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (295, 811, 1321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3373) [M::mem_pestat] mean and std.dev: (803.71, 572.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4399) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 910.727 CPU sec, 53.436 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (81, 667546, 16, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (40, 74, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 430) [M::mem_pestat] mean and std.dev: (95.58, 72.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 560) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.39, 75.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (91, 139, 617) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1669) [M::mem_pestat] mean and std.dev: (304.20, 470.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2195) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (613, 1015, 1770) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4084) [M::mem_pestat] mean and std.dev: (1016.79, 695.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5241) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 904.818 CPU sec, 40.212 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (73, 666544, 24, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (44, 86, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (102.14, 86.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 664) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.37, 75.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (50, 118, 3607) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10721) [M::mem_pestat] mean and std.dev: (1677.08, 2506.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14278) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (419, 814, 1614) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4004) [M::mem_pestat] mean and std.dev: (1058.59, 914.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5199) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 910.427 CPU sec, 52.838 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 667349, 27, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (38, 64, 115) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 269) [M::mem_pestat] mean and std.dev: (78.89, 58.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 346) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.36, 75.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (45, 97, 1366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4008) [M::mem_pestat] mean and std.dev: (558.16, 980.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5329) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (532, 784, 915) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1681) [M::mem_pestat] mean and std.dev: (683.00, 240.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2064) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 903.434 CPU sec, 40.006 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (72, 666409, 26, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (35, 59, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 428) [M::mem_pestat] mean and std.dev: (75.07, 69.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 559) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.44, 75.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (54, 105, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 393) [M::mem_pestat] mean and std.dev: (92.36, 57.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 506) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (297, 1097, 2045) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5541) [M::mem_pestat] mean and std.dev: (1050.45, 989.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7289) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 912.327 CPU sec, 53.762 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (82, 667321, 27, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (41, 77, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479) [M::mem_pestat] mean and std.dev: (112.71, 103.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 625) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.34, 75.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 66, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 973) [M::mem_pestat] mean and std.dev: (87.95, 116.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1288) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (612, 802, 1448) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3120) [M::mem_pestat] mean and std.dev: (915.20, 704.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3956) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 906.033 CPU sec, 40.239 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (74, 666963, 30, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (32, 62, 153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 395) [M::mem_pestat] mean and std.dev: (87.00, 71.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 516) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.42, 75.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (49, 128, 1544) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4534) [M::mem_pestat] mean and std.dev: (667.67, 1155.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6029) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (224, 756, 1067) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2753) [M::mem_pestat] mean and std.dev: (722.00, 455.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3596) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 909.484 CPU sec, 52.873 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (58, 667284, 29, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (43, 79, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (100.92, 77.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 647) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.38, 75.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 92, 335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 957) [M::mem_pestat] mean and std.dev: (106.33, 167.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1268) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (671, 1265, 1719) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3815) [M::mem_pestat] mean and std.dev: (1072.33, 622.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4863) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 905.105 CPU sec, 40.310 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (65, 667406, 28, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (40, 67, 140) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 340) [M::mem_pestat] mean and std.dev: (83.44, 61.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 440) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.42, 75.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 107, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 459) [M::mem_pestat] mean and std.dev: (69.27, 51.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (661, 1068, 1800) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4078) [M::mem_pestat] mean and std.dev: (1351.86, 1057.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5581) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 904.977 CPU sec, 52.910 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (62, 666976, 20, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (34, 65, 144) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 364) [M::mem_pestat] mean and std.dev: (76.73, 59.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 474) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 262, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.32, 75.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 62, 2666) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7936) [M::mem_pestat] mean and std.dev: (1064.65, 1799.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10571) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 904.100 CPU sec, 40.207 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (81, 666847, 21, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (39, 67, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (86.67, 74.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 531) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.34, 75.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 95, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1034) [M::mem_pestat] mean and std.dev: (83.00, 83.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1370) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (867, 1017, 2534) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5868) [M::mem_pestat] mean and std.dev: (1623.69, 1416.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7535) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 901.490 CPU sec, 47.427 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (84, 667011, 22, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (44, 71, 139) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 329) [M::mem_pestat] mean and std.dev: (84.28, 57.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 424) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.34, 75.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (46, 71, 153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 367) [M::mem_pestat] mean and std.dev: (75.50, 45.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 474) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (902, 1118, 1857) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3767) [M::mem_pestat] mean and std.dev: (1304.13, 661.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4722) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 909.339 CPU sec, 52.655 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (65, 666859, 27, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (51, 101, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (108.45, 88.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 675) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.34, 75.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 34, 94) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 242) [M::mem_pestat] mean and std.dev: (36.86, 30.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 316) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (183, 717, 1683) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4683) [M::mem_pestat] mean and std.dev: (788.15, 655.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6183) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 909.078 CPU sec, 40.688 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (64, 667037, 26, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (40, 70, 148) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 364) [M::mem_pestat] mean and std.dev: (92.95, 72.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 472) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.31, 75.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 83, 775) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2271) [M::mem_pestat] mean and std.dev: (240.59, 489.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3019) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (705, 1155, 1664) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3582) [M::mem_pestat] mean and std.dev: (1229.10, 749.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4541) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 896.343 CPU sec, 52.407 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (77, 667242, 34, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (37, 63, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (84.84, 68.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 549) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.49, 75.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 58, 600) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1758) [M::mem_pestat] mean and std.dev: (214.60, 371.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2337) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (570, 726, 1672) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3876) [M::mem_pestat] mean and std.dev: (778.21, 610.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4978) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 902.293 CPU sec, 40.133 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (75, 666056, 39, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (47, 112, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 566) [M::mem_pestat] mean and std.dev: (118.24, 88.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 739) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.34, 75.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (52, 100, 3268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9700) [M::mem_pestat] mean and std.dev: (1159.89, 1859.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12916) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (681, 1054, 1289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2505) [M::mem_pestat] mean and std.dev: (933.50, 437.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3113) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 905.817 CPU sec, 51.413 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (67, 666977, 29, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (41, 59, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (86.70, 71.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 617) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (11, 521) [M::mem_pestat] mean and std.dev: (269.31, 75.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (52, 129, 1032) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2992) [M::mem_pestat] mean and std.dev: (321.24, 629.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3972) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (776, 1002, 2642) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6374) [M::mem_pestat] mean and std.dev: (1446.72, 1068.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8240) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 899.990 CPU sec, 40.033 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (70, 666833, 32, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (37, 62, 128) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 310) [M::mem_pestat] mean and std.dev: (74.08, 59.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 401) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.34, 75.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (39, 128, 1289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3789) [M::mem_pestat] mean and std.dev: (544.97, 818.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5039) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (207, 741, 1160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3066) [M::mem_pestat] mean and std.dev: (679.77, 542.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4019) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 900.994 CPU sec, 51.944 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (79, 666827, 27, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (49, 84, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (103.36, 76.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 529) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.39, 75.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (44, 125, 1582) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4658) [M::mem_pestat] mean and std.dev: (873.81, 1375.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6375) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (374, 758, 1026) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2330) [M::mem_pestat] mean and std.dev: (756.69, 440.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2982) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 898.127 CPU sec, 39.781 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (79, 667250, 25, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (46, 77, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424) [M::mem_pestat] mean and std.dev: (86.01, 58.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 550) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.38, 75.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 66, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 447) [M::mem_pestat] mean and std.dev: (64.70, 49.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (601, 806, 1542) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3424) [M::mem_pestat] mean and std.dev: (1050.71, 865.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4513) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 900.945 CPU sec, 51.686 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (83, 666549, 32, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (41, 61, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (105.63, 93.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 641) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.47, 75.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 78, 1911) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5695) [M::mem_pestat] mean and std.dev: (774.62, 1311.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7587) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (859, 1097, 1394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2464) [M::mem_pestat] mean and std.dev: (1044.78, 551.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3250) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 906.130 CPU sec, 40.307 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (79, 667401, 26, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (45, 69, 126) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 288) [M::mem_pestat] mean and std.dev: (79.11, 54.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 369) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.43, 75.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (41, 126, 2599) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7715) [M::mem_pestat] mean and std.dev: (1302.92, 1753.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10273) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (538, 959, 1433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3223) [M::mem_pestat] mean and std.dev: (1061.38, 857.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4490) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 900.221 CPU sec, 52.167 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (78, 666522, 30, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (39, 56, 124) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 294) [M::mem_pestat] mean and std.dev: (81.89, 63.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 379) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.37, 75.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (44, 78, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926) [M::mem_pestat] mean and std.dev: (73.09, 70.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1220) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 901.695 CPU sec, 40.051 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (71, 667264, 30, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (44, 65, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 446) [M::mem_pestat] mean and std.dev: (95.77, 73.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 580) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.33, 75.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 126, 1781) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5301) [M::mem_pestat] mean and std.dev: (734.41, 1140.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7061) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (64, 344, 859) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2449) [M::mem_pestat] mean and std.dev: (446.06, 407.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3244) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 893.022 CPU sec, 50.964 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (64, 666458, 30, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (41, 70, 158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 392) [M::mem_pestat] mean and std.dev: (92.90, 71.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 509) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.32, 75.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 64, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (79.38, 96.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 662) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (681, 937, 1914) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4380) [M::mem_pestat] mean and std.dev: (1158.82, 682.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5613) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 900.164 CPU sec, 39.944 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (90, 666979, 17, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (43, 93, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682) [M::mem_pestat] mean and std.dev: (122.03, 126.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 895) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.45, 75.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 54, 113) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 311) [M::mem_pestat] mean and std.dev: (68.69, 65.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 410) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (778, 1363, 2574) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6166) [M::mem_pestat] mean and std.dev: (1854.20, 1473.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7962) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 903.260 CPU sec, 44.987 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (69, 666730, 25, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (41, 63, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476) [M::mem_pestat] mean and std.dev: (96.30, 92.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 621) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.37, 75.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (40, 116, 1930) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5710) [M::mem_pestat] mean and std.dev: (1245.52, 1710.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8086) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (698, 1044, 1594) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3386) [M::mem_pestat] mean and std.dev: (907.06, 387.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4282) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 910.330 CPU sec, 53.303 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (75, 667037, 32, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (45, 76, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 429) [M::mem_pestat] mean and std.dev: (98.94, 82.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 557) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.41, 75.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (44, 87, 2963) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8801) [M::mem_pestat] mean and std.dev: (1299.25, 2038.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11720) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (688, 976, 2144) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5056) [M::mem_pestat] mean and std.dev: (1063.70, 693.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6512) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 904.347 CPU sec, 40.243 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (88, 666623, 35, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (38, 63, 143) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 353) [M::mem_pestat] mean and std.dev: (82.65, 63.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 458) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.57, 75.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (50, 130, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 452) [M::mem_pestat] mean and std.dev: (114.33, 91.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 903.073 CPU sec, 52.190 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (83, 667239, 29, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (44, 70, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 524) [M::mem_pestat] mean and std.dev: (110.22, 104.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 684) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.38, 75.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 69, 635) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1855) [M::mem_pestat] mean and std.dev: (245.62, 434.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2465) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (502, 925, 1128) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2380) [M::mem_pestat] mean and std.dev: (691.85, 425.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3006) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 899.669 CPU sec, 39.875 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (72, 666812, 32, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (37, 63, 138) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 340) [M::mem_pestat] mean and std.dev: (78.21, 59.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 441) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.35, 75.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (42, 124, 1169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3423) [M::mem_pestat] mean and std.dev: (436.17, 827.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4550) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (866, 1073, 2084) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4520) [M::mem_pestat] mean and std.dev: (1588.80, 1170.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6272) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 902.942 CPU sec, 52.183 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (81, 666652, 31, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (37, 67, 158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400) [M::mem_pestat] mean and std.dev: (80.93, 56.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 521) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 262, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (11, 521) [M::mem_pestat] mean and std.dev: (269.16, 75.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (39, 94, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 540) [M::mem_pestat] mean and std.dev: (95.24, 103.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 707) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (981, 1277, 1511) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2571) [M::mem_pestat] mean and std.dev: (1103.93, 409.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3101) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 902.808 CPU sec, 40.083 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (71, 666856, 29, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (41, 67, 147) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 359) [M::mem_pestat] mean and std.dev: (92.05, 78.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 465) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.52, 75.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 67, 469) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1355) [M::mem_pestat] mean and std.dev: (99.71, 126.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1798) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (623, 892, 1441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3077) [M::mem_pestat] mean and std.dev: (985.20, 783.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4120) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 905.836 CPU sec, 52.647 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (75, 666896, 24, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (41, 77, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 602) [M::mem_pestat] mean and std.dev: (103.58, 95.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 789) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.27, 75.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (57, 126, 2575) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7611) [M::mem_pestat] mean and std.dev: (1071.96, 1819.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10129) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (655, 890, 1182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2236) [M::mem_pestat] mean and std.dev: (797.45, 496.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2783) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 899.676 CPU sec, 40.022 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (71, 667292, 28, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (41, 74, 151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 371) [M::mem_pestat] mean and std.dev: (90.58, 69.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 481) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.30, 75.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (43, 67, 156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 382) [M::mem_pestat] mean and std.dev: (73.13, 54.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 495) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 910.011 CPU sec, 52.545 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (75, 666941, 29, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (48, 74, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 432) [M::mem_pestat] mean and std.dev: (86.63, 63.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 560) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.39, 75.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 133, 377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1057) [M::mem_pestat] mean and std.dev: (110.92, 102.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1397) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (428, 798, 1397) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3335) [M::mem_pestat] mean and std.dev: (861.00, 484.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4304) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 906.664 CPU sec, 40.323 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (84, 666997, 25, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (42, 71, 157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 387) [M::mem_pestat] mean and std.dev: (80.62, 57.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 502) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 262, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.31, 75.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 46, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 994) [M::mem_pestat] mean and std.dev: (137.77, 223.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1318) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (722, 975, 1613) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3395) [M::mem_pestat] mean and std.dev: (1061.78, 760.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4286) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 911.514 CPU sec, 52.519 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (60, 666704, 43, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (41, 85, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 593) [M::mem_pestat] mean and std.dev: (109.44, 124.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 777) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.26, 75.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 88, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 859) [M::mem_pestat] mean and std.dev: (94.26, 106.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1135) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (612, 721, 997) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1767) [M::mem_pestat] mean and std.dev: (697.79, 394.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2276) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 901.849 CPU sec, 40.060 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (93, 666609, 38, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (42, 69, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (96.25, 81.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 618) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.26, 75.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 77, 930) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2736) [M::mem_pestat] mean and std.dev: (314.30, 600.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3639) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 908.003 CPU sec, 52.317 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (71, 666511, 34, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (45, 65, 143) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 339) [M::mem_pestat] mean and std.dev: (76.48, 54.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 437) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.24, 75.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 150, 1259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3715) [M::mem_pestat] mean and std.dev: (375.72, 502.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4943) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 909.358 CPU sec, 40.426 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (79, 666650, 29, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (40, 71, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 427) [M::mem_pestat] mean and std.dev: (104.64, 85.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 556) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.50, 75.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 123, 373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1055) [M::mem_pestat] mean and std.dev: (148.00, 193.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1396) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 903.647 CPU sec, 42.978 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (68, 666851, 36, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (53, 97, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (109.48, 89.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 601) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.34, 75.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 130, 2864) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8526) [M::mem_pestat] mean and std.dev: (1474.11, 2270.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11357) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (876, 999, 2051) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4401) [M::mem_pestat] mean and std.dev: (1240.22, 663.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5576) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 905.676 CPU sec, 51.198 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (60, 666585, 30, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (48, 100, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402) [M::mem_pestat] mean and std.dev: (109.09, 82.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 520) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.39, 75.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (41, 100, 513) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1457) [M::mem_pestat] mean and std.dev: (101.48, 119.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1929) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 905.993 CPU sec, 40.035 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (84, 667306, 26, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (35, 62, 125) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 305) [M::mem_pestat] mean and std.dev: (80.73, 69.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 395) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.34, 75.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 97, 145) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 371) [M::mem_pestat] mean and std.dev: (68.80, 45.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 484) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (595, 1005, 1917) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4561) [M::mem_pestat] mean and std.dev: (1171.31, 997.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5883) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 909.608 CPU sec, 52.673 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (84, 666896, 34, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (41, 82, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 533) [M::mem_pestat] mean and std.dev: (99.25, 77.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 697) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.30, 75.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (39, 63, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 504) [M::mem_pestat] mean and std.dev: (79.30, 78.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 659) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1329, 2298, 3201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6945) [M::mem_pestat] mean and std.dev: (2196.60, 1202.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8817) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 908.571 CPU sec, 40.315 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (61, 667531, 25, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (39, 90, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (94.96, 68.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.31, 75.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 50, 112) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 306) [M::mem_pestat] mean and std.dev: (54.27, 60.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 403) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (784, 1134, 2240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5152) [M::mem_pestat] mean and std.dev: (1435.59, 932.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6608) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 907.910 CPU sec, 53.932 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (76, 666363, 22, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (51, 100, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747) [M::mem_pestat] mean and std.dev: (130.17, 139.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 979) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.34, 75.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (39, 166, 685) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1977) [M::mem_pestat] mean and std.dev: (227.17, 406.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2623) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (248, 905, 1675) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4529) [M::mem_pestat] mean and std.dev: (1050.71, 864.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5956) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 906.245 CPU sec, 40.485 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (67, 667407, 23, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (44, 66, 123) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 281) [M::mem_pestat] mean and std.dev: (83.56, 58.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 360) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.52, 75.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (62, 127, 1068) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3080) [M::mem_pestat] mean and std.dev: (516.10, 770.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4086) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1273, 1834, 2183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4003) [M::mem_pestat] mean and std.dev: (1747.21, 969.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5625) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 898.687 CPU sec, 52.385 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (65, 667449, 25, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (47, 73, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662) [M::mem_pestat] mean and std.dev: (130.72, 155.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 867) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.31, 75.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (48, 110, 965) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2799) [M::mem_pestat] mean and std.dev: (249.81, 486.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3716) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (965, 1359, 3934) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9872) [M::mem_pestat] mean and std.dev: (2241.18, 2236.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12841) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 901.543 CPU sec, 39.913 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (87, 666978, 28, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (46, 79, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 589) [M::mem_pestat] mean and std.dev: (108.88, 102.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 770) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.40, 75.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 47, 141) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 379) [M::mem_pestat] mean and std.dev: (56.35, 51.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 498) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (600, 898, 1516) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3348) [M::mem_pestat] mean and std.dev: (1026.47, 851.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4432) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 904.610 CPU sec, 51.540 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (75, 666781, 34, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (44, 64, 148) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 356) [M::mem_pestat] mean and std.dev: (82.71, 66.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 460) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.45, 75.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 65, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760) [M::mem_pestat] mean and std.dev: (82.41, 136.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1004) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 907.280 CPU sec, 40.267 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (64, 666970, 25, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (36, 79, 126) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 306) [M::mem_pestat] mean and std.dev: (82.24, 63.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 396) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 262, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.27, 75.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 52, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 870) [M::mem_pestat] mean and std.dev: (136.54, 172.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1151) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (851, 1319, 2005) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4313) [M::mem_pestat] mean and std.dev: (1200.42, 645.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5467) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 908.201 CPU sec, 51.898 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (78, 666870, 28, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (42, 75, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 447) [M::mem_pestat] mean and std.dev: (94.47, 74.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 582) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.38, 75.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 123, 1167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3429) [M::mem_pestat] mean and std.dev: (557.78, 837.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4560) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 904.915 CPU sec, 40.206 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (69, 667035, 28, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (39, 65, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (92.13, 77.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.57, 75.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (47, 92, 1692) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4982) [M::mem_pestat] mean and std.dev: (938.39, 1388.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6627) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (645, 1096, 1631) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3603) [M::mem_pestat] mean and std.dev: (1050.08, 583.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4589) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 907.093 CPU sec, 51.873 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (75, 667142, 29, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (36, 62, 112) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 264) [M::mem_pestat] mean and std.dev: (66.32, 44.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 340) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.41, 75.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (56, 138, 2229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6575) [M::mem_pestat] mean and std.dev: (981.32, 1630.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8748) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 895, 1413) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3845) [M::mem_pestat] mean and std.dev: (958.40, 791.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5061) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 897.934 CPU sec, 39.763 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (83, 666871, 26, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (42, 69, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 465) [M::mem_pestat] mean and std.dev: (102.55, 90.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.47, 75.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 138, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 740) [M::mem_pestat] mean and std.dev: (104.70, 77.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 976) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (571, 1135, 2681) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6901) [M::mem_pestat] mean and std.dev: (1497.25, 1256.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9011) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 894.786 CPU sec, 42.043 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (75, 666850, 37, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (44, 67, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 392) [M::mem_pestat] mean and std.dev: (91.03, 72.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 508) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.45, 75.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 113, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 532) [M::mem_pestat] mean and std.dev: (83.63, 81.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 700) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 916.336 CPU sec, 53.399 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (86, 667198, 27, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (36, 67, 140) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 348) [M::mem_pestat] mean and std.dev: (87.22, 70.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 452) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.54, 75.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 85, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567) [M::mem_pestat] mean and std.dev: (84.05, 107.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 747) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (207, 704, 2142) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6012) [M::mem_pestat] mean and std.dev: (955.65, 886.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7947) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 904.164 CPU sec, 40.164 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (78, 666454, 28, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (46, 74, 156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 376) [M::mem_pestat] mean and std.dev: (96.47, 70.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 486) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.36, 75.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 124, 917) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2689) [M::mem_pestat] mean and std.dev: (324.58, 631.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3575) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (651, 1182, 2223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5367) [M::mem_pestat] mean and std.dev: (1291.27, 1051.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6939) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 912.369 CPU sec, 54.086 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (87, 667008, 37, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (46, 80, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (97.62, 79.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 650) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.28, 75.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 98, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585) [M::mem_pestat] mean and std.dev: (98.16, 98.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 768) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (821, 950, 1584) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3110) [M::mem_pestat] mean and std.dev: (999.64, 781.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4125) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 906.127 CPU sec, 40.299 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (82, 666962, 30, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (43, 77, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 541) [M::mem_pestat] mean and std.dev: (99.59, 82.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 707) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.35, 75.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (38, 72, 940) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2744) [M::mem_pestat] mean and std.dev: (373.15, 657.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3646) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (274, 922, 1672) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4468) [M::mem_pestat] mean and std.dev: (1012.83, 1022.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5866) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 906.814 CPU sec, 53.603 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (74, 667509, 29, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (39, 90, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 579) [M::mem_pestat] mean and std.dev: (107.78, 110.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 759) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.55, 75.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 50, 85) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 213) [M::mem_pestat] mean and std.dev: (47.31, 31.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 277) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (757, 940, 1797) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3877) [M::mem_pestat] mean and std.dev: (1097.18, 708.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4917) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 903.322 CPU sec, 40.240 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (77, 666834, 30, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (38, 62, 143) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 353) [M::mem_pestat] mean and std.dev: (82.97, 65.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 458) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 262, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.27, 75.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 84, 1586) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4690) [M::mem_pestat] mean and std.dev: (881.07, 1558.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7115) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (444, 1102, 1472) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3528) [M::mem_pestat] mean and std.dev: (1081.00, 837.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4556) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 909.271 CPU sec, 53.909 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (77, 666642, 32, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (39, 60, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 450) [M::mem_pestat] mean and std.dev: (97.84, 91.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 587) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (216, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (12, 522) [M::mem_pestat] mean and std.dev: (269.47, 75.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 624) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (48, 63, 1803) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5313) [M::mem_pestat] mean and std.dev: (801.23, 1438.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7068) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (212, 967, 1519) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4133) [M::mem_pestat] mean and std.dev: (829.69, 595.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5440) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 904.169 CPU sec, 40.404 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (58, 666929, 32, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (37, 61, 150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 376) [M::mem_pestat] mean and std.dev: (82.35, 66.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 489) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.43, 75.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (54, 121, 2584) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7644) [M::mem_pestat] mean and std.dev: (1144.41, 1629.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10174) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (676, 995, 2336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5656) [M::mem_pestat] mean and std.dev: (1465.42, 1317.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7316) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 912.094 CPU sec, 53.008 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (77, 667491, 29, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (41, 59, 155) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 383) [M::mem_pestat] mean and std.dev: (88.53, 76.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 497) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.33, 75.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (52, 80, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1420) [M::mem_pestat] mean and std.dev: (216.46, 365.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1876) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (958, 1002, 2643) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6013) [M::mem_pestat] mean and std.dev: (1309.50, 869.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7698) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 902.374 CPU sec, 40.139 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (77, 666570, 40, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (47, 77, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (98.24, 82.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.35, 75.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 56, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (59.72, 51.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 615) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (195, 707, 1214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3252) [M::mem_pestat] mean and std.dev: (838.05, 808.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4271) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 906.242 CPU sec, 52.269 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (77, 666329, 19, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (37, 56, 153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 385) [M::mem_pestat] mean and std.dev: (85.85, 73.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 501) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.30, 75.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 46, 94) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 242) [M::mem_pestat] mean and std.dev: (44.88, 32.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 316) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 903.170 CPU sec, 40.010 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (71, 667336, 26, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (46, 72, 148) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 352) [M::mem_pestat] mean and std.dev: (92.86, 73.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 454) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.49, 75.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (42, 108, 2857) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8487) [M::mem_pestat] mean and std.dev: (1131.88, 1664.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11302) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (554, 814, 1197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2483) [M::mem_pestat] mean and std.dev: (718.78, 380.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3126) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 909.358 CPU sec, 52.466 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (63, 666723, 37, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (38, 54, 99) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 221) [M::mem_pestat] mean and std.dev: (62.24, 37.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 282) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.36, 75.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 66, 1489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4397) [M::mem_pestat] mean and std.dev: (492.85, 1013.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5851) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1124, 1443, 1666) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (40, 2750) [M::mem_pestat] mean and std.dev: (1316.67, 696.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4103) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 910.562 CPU sec, 40.749 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (71, 666367, 25, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (51, 124, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657) [M::mem_pestat] mean and std.dev: (113.90, 103.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 859) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.39, 75.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (41, 124, 2065) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6113) [M::mem_pestat] mean and std.dev: (793.87, 1359.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8137) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (795, 966, 1433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2709) [M::mem_pestat] mean and std.dev: (970.80, 427.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3347) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 910.442 CPU sec, 52.417 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (64, 666913, 27, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (48, 96, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 555) [M::mem_pestat] mean and std.dev: (107.38, 84.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 724) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.33, 75.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 57, 155) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (54.76, 45.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 530) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (751, 983, 2019) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4555) [M::mem_pestat] mean and std.dev: (1339.18, 1241.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6307) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 907.373 CPU sec, 40.269 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (75, 666716, 28, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (45, 82, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 426) [M::mem_pestat] mean and std.dev: (90.09, 63.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 553) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 262, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.40, 75.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (38, 85, 1219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3581) [M::mem_pestat] mean and std.dev: (406.08, 854.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4762) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (610, 1148, 2076) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5008) [M::mem_pestat] mean and std.dev: (1344.64, 1116.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6474) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 913.857 CPU sec, 53.759 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (68, 667503, 32, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (41, 61, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 404) [M::mem_pestat] mean and std.dev: (96.29, 82.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 525) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.33, 75.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (39, 111, 333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921) [M::mem_pestat] mean and std.dev: (81.92, 76.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1215) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 905.220 CPU sec, 40.329 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (88, 666746, 30, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (42, 83, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 546) [M::mem_pestat] mean and std.dev: (114.06, 100.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 714) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.45, 75.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (41, 141, 1691) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4991) [M::mem_pestat] mean and std.dev: (567.23, 1078.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6641) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (845, 1248, 1829) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3797) [M::mem_pestat] mean and std.dev: (1325.50, 779.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4781) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 912.415 CPU sec, 53.271 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (71, 666361, 21, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (39, 55, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (73.57, 63.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 615) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.41, 75.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 80, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (73.53, 51.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 629) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (612, 975, 1370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2886) [M::mem_pestat] mean and std.dev: (920.33, 539.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3644) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 913.946 CPU sec, 40.729 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (65, 667019, 36, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (43, 102, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (91.19, 65.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.56, 75.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 88, 383) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1089) [M::mem_pestat] mean and std.dev: (72.57, 75.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1442) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (301, 687, 1140) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2818) [M::mem_pestat] mean and std.dev: (613.10, 393.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3657) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 915.526 CPU sec, 53.484 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (78, 666835, 24, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (38, 76, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 452) [M::mem_pestat] mean and std.dev: (86.82, 76.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.43, 75.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (48, 93, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906) [M::mem_pestat] mean and std.dev: (119.00, 119.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1192) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (675, 1050, 2573) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6369) [M::mem_pestat] mean and std.dev: (993.00, 724.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8267) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 911.664 CPU sec, 40.861 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (73, 666598, 38, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (41, 68, 122) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 284) [M::mem_pestat] mean and std.dev: (79.48, 59.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 365) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (216, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (12, 522) [M::mem_pestat] mean and std.dev: (269.33, 75.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 624) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 88, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933) [M::mem_pestat] mean and std.dev: (89.90, 133.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1235) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (636, 915, 2056) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4896) [M::mem_pestat] mean and std.dev: (1261.93, 1035.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6316) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 911.751 CPU sec, 51.944 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (83, 666608, 19, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (37, 64, 158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400) [M::mem_pestat] mean and std.dev: (86.47, 69.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 521) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.45, 75.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (45, 102, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 702) [M::mem_pestat] mean and std.dev: (87.53, 62.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 921) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (419, 783, 2451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6515) [M::mem_pestat] mean and std.dev: (972.08, 895.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8547) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 914.339 CPU sec, 40.631 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (69, 666531, 29, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (38, 50, 122) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 290) [M::mem_pestat] mean and std.dev: (73.15, 62.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 374) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.46, 75.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 81, 396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1126) [M::mem_pestat] mean and std.dev: (82.65, 81.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1491) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (819, 1555, 2372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5478) [M::mem_pestat] mean and std.dev: (1550.88, 906.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7031) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 914.633 CPU sec, 52.770 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (72, 667280, 33, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 106, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 441) [M::mem_pestat] mean and std.dev: (114.55, 82.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 570) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.43, 75.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (44, 114, 468) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1316) [M::mem_pestat] mean and std.dev: (124.63, 195.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1740) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 901.831 CPU sec, 40.061 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (67, 666957, 29, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 86, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 535) [M::mem_pestat] mean and std.dev: (114.77, 97.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 695) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.49, 75.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (40, 76, 1828) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5404) [M::mem_pestat] mean and std.dev: (780.07, 1475.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7192) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (294, 1080, 1604) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4224) [M::mem_pestat] mean and std.dev: (1019.92, 725.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5534) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 907.673 CPU sec, 52.662 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (58, 667039, 31, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (46, 69, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646) [M::mem_pestat] mean and std.dev: (102.63, 96.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 846) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.47, 75.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (39, 69, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492) [M::mem_pestat] mean and std.dev: (93.74, 81.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 643) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 906.359 CPU sec, 40.303 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (92, 666514, 29, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (44, 73, 151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 365) [M::mem_pestat] mean and std.dev: (90.55, 69.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 472) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.44, 75.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 117, 1907) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5655) [M::mem_pestat] mean and std.dev: (927.86, 1444.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7529) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (108, 574, 918) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2538) [M::mem_pestat] mean and std.dev: (642.50, 518.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3348) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 906.344 CPU sec, 53.185 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (70, 666826, 22, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (44, 76, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488) [M::mem_pestat] mean and std.dev: (104.61, 91.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 636) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.58, 75.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 82, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 419) [M::mem_pestat] mean and std.dev: (80.67, 75.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 547) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (436, 682, 1495) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3613) [M::mem_pestat] mean and std.dev: (901.46, 732.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4672) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 913.173 CPU sec, 53.239 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (74, 666336, 25, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (44, 83, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (98.85, 73.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 592) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.31, 75.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (46, 91, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697) [M::mem_pestat] mean and std.dev: (89.38, 73.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 914) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (536, 1154, 2774) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7250) [M::mem_pestat] mean and std.dev: (1621.59, 1635.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9488) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 903.950 CPU sec, 40.206 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (57, 666508, 24, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (37, 62, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (114.06, 120.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 781) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.36, 75.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 72, 140) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 386) [M::mem_pestat] mean and std.dev: (67.19, 60.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 509) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1045, 1215, 2037) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4021) [M::mem_pestat] mean and std.dev: (1472.29, 839.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5013) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 911.279 CPU sec, 53.376 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (70, 667030, 37, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (42, 61, 145) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 351) [M::mem_pestat] mean and std.dev: (77.15, 54.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 454) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.46, 75.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 56, 1665) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4945) [M::mem_pestat] mean and std.dev: (742.63, 1230.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6585) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1075, 1388, 2162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4336) [M::mem_pestat] mean and std.dev: (1578.91, 1062.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5831) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 907.585 CPU sec, 40.339 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (68, 666424, 31, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (38, 88, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 404) [M::mem_pestat] mean and std.dev: (89.34, 61.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 526) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.40, 75.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 78, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629) [M::mem_pestat] mean and std.dev: (68.79, 61.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 827) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (642, 1197, 1909) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4443) [M::mem_pestat] mean and std.dev: (1156.33, 778.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5710) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 911.480 CPU sec, 53.859 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (81, 666867, 30, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (45, 81, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492) [M::mem_pestat] mean and std.dev: (109.68, 89.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 641) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.43, 75.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 74, 477) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1381) [M::mem_pestat] mean and std.dev: (190.31, 348.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1833) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (844, 1192, 1691) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3385) [M::mem_pestat] mean and std.dev: (1178.60, 647.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4232) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 906.376 CPU sec, 40.235 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (56, 666991, 32, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (45, 86, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 489) [M::mem_pestat] mean and std.dev: (107.92, 92.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 637) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.28, 75.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (43, 131, 669) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1921) [M::mem_pestat] mean and std.dev: (280.00, 431.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2547) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (471, 1009, 2354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6120) [M::mem_pestat] mean and std.dev: (1413.78, 1280.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8003) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 909.334 CPU sec, 52.473 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (71, 666694, 31, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (43, 68, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 553) [M::mem_pestat] mean and std.dev: (104.10, 106.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 723) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.29, 75.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (67, 142, 2985) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8821) [M::mem_pestat] mean and std.dev: (1552.32, 2351.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11739) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (649, 1133, 1815) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4147) [M::mem_pestat] mean and std.dev: (987.36, 577.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5313) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 906.522 CPU sec, 40.401 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (69, 666891, 26, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (37, 57, 142) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 352) [M::mem_pestat] mean and std.dev: (88.05, 75.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 457) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.48, 75.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (48, 107, 347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945) [M::mem_pestat] mean and std.dev: (140.86, 165.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1244) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (522, 785, 1160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2436) [M::mem_pestat] mean and std.dev: (776.69, 457.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3074) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 907.927 CPU sec, 51.820 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (78, 667219, 25, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (34, 60, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 427) [M::mem_pestat] mean and std.dev: (79.25, 67.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 558) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.45, 75.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 89, 336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948) [M::mem_pestat] mean and std.dev: (91.30, 84.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1254) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 903.824 CPU sec, 40.109 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (82, 666755, 15, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (42, 79, 157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 387) [M::mem_pestat] mean and std.dev: (93.65, 71.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 502) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.43, 75.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 52, 113) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 273) [M::mem_pestat] mean and std.dev: (47.67, 33.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 353) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (782, 1404, 2357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5507) [M::mem_pestat] mean and std.dev: (1588.20, 1150.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7082) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 915.233 CPU sec, 52.997 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (54, 666723, 28, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (42, 67, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 546) [M::mem_pestat] mean and std.dev: (94.70, 96.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 714) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.42, 75.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 67, 966) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2842) [M::mem_pestat] mean and std.dev: (465.44, 702.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3780) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 906.333 CPU sec, 40.143 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (59, 666955, 36, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (42, 58, 133) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 315) [M::mem_pestat] mean and std.dev: (84.16, 66.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 406) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.32, 75.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 84, 1302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3844) [M::mem_pestat] mean and std.dev: (429.21, 729.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5115) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (661, 1225, 1712) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3814) [M::mem_pestat] mean and std.dev: (1002.45, 689.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4865) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 908.943 CPU sec, 53.722 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (88, 667124, 30, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (39, 70, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 525) [M::mem_pestat] mean and std.dev: (104.62, 100.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 687) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 262, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.26, 75.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 100, 1809) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5359) [M::mem_pestat] mean and std.dev: (975.43, 1551.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7181) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 905.679 CPU sec, 40.354 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (84, 667013, 33, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (39, 68, 139) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 339) [M::mem_pestat] mean and std.dev: (87.05, 64.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 439) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.41, 75.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 96, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 439) [M::mem_pestat] mean and std.dev: (72.81, 51.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 576) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (792, 1080, 1878) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4050) [M::mem_pestat] mean and std.dev: (978.12, 445.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5136) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 904.564 CPU sec, 52.142 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (75, 666860, 29, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (48, 80, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 390) [M::mem_pestat] mean and std.dev: (101.37, 68.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 504) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.49, 75.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (43, 115, 900) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2614) [M::mem_pestat] mean and std.dev: (312.11, 465.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3471) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 914.507 CPU sec, 54.120 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (73, 666742, 28, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (45, 81, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 498) [M::mem_pestat] mean and std.dev: (96.44, 77.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 649) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.41, 75.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (47, 99, 1778) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5240) [M::mem_pestat] mean and std.dev: (591.04, 1074.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6971) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (811, 930, 1075) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (283, 1603) [M::mem_pestat] mean and std.dev: (975.00, 149.38) [M::mem_pestat] low and high boundaries for proper pairs: (19, 1867) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 909.278 CPU sec, 40.436 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (63, 665831, 27, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (37, 53, 137) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 337) [M::mem_pestat] mean and std.dev: (83.15, 70.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 437) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.28, 75.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 57, 145) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 365) [M::mem_pestat] mean and std.dev: (80.91, 73.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 475) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 916.468 CPU sec, 52.816 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (78, 667109, 26, 23) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (41, 69, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 614) [M::mem_pestat] mean and std.dev: (116.54, 100.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 805) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.40, 75.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (47, 92, 1840) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5426) [M::mem_pestat] mean and std.dev: (980.28, 1586.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7327) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (763, 889, 1404) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2686) [M::mem_pestat] mean and std.dev: (1052.95, 637.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3603) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 909.381 CPU sec, 40.548 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (62, 666611, 31, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (46, 93, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454) [M::mem_pestat] mean and std.dev: (111.86, 86.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.29, 75.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (41, 52, 149) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 365) [M::mem_pestat] mean and std.dev: (61.62, 46.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 473) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 908.151 CPU sec, 52.898 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (69, 666342, 23, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (35, 55, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 485) [M::mem_pestat] mean and std.dev: (88.19, 91.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.34, 75.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (57, 104, 595) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1671) [M::mem_pestat] mean and std.dev: (204.25, 270.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2209) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1097, 1373, 4341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10829) [M::mem_pestat] mean and std.dev: (2635.73, 2482.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14073) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 905.571 CPU sec, 40.313 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (71, 666450, 29, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (41, 67, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 428) [M::mem_pestat] mean and std.dev: (95.58, 78.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 557) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (11, 521) [M::mem_pestat] mean and std.dev: (269.26, 75.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 114, 608) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1764) [M::mem_pestat] mean and std.dev: (272.56, 449.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2342) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (636, 718, 994) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1710) [M::mem_pestat] mean and std.dev: (773.46, 279.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2068) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 907.977 CPU sec, 53.628 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (72, 666933, 26, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (40, 68, 159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 397) [M::mem_pestat] mean and std.dev: (84.34, 71.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 516) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.44, 75.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 58, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 430) [M::mem_pestat] mean and std.dev: (70.77, 60.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 562) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (570, 879, 1343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2889) [M::mem_pestat] mean and std.dev: (937.93, 558.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3662) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 910.219 CPU sec, 40.427 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (59, 667310, 37, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (41, 91, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 515) [M::mem_pestat] mean and std.dev: (105.02, 104.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 673) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.38, 75.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (46, 89, 2746) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8146) [M::mem_pestat] mean and std.dev: (1224.64, 1829.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10846) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (140, 330, 1330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3710) [M::mem_pestat] mean and std.dev: (721.21, 818.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4900) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 905.310 CPU sec, 52.603 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (66, 666816, 27, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (45, 75, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (98.20, 77.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 621) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.28, 75.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 175, 1946) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5768) [M::mem_pestat] mean and std.dev: (1002.16, 1594.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7679) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (421, 834, 1755) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4423) [M::mem_pestat] mean and std.dev: (1043.67, 877.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5757) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 902.607 CPU sec, 40.019 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (60, 666417, 21, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (50, 99, 432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1196) [M::mem_pestat] mean and std.dev: (188.60, 224.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1578) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.35, 75.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (41, 58, 137) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 329) [M::mem_pestat] mean and std.dev: (69.50, 54.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 425) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (588, 874, 2093) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5103) [M::mem_pestat] mean and std.dev: (1051.33, 768.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6608) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 914.857 CPU sec, 52.493 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (78, 666780, 39, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (47, 97, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 554) [M::mem_pestat] mean and std.dev: (115.32, 97.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 723) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.49, 75.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (41, 117, 496) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1406) [M::mem_pestat] mean and std.dev: (197.15, 295.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1861) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (339, 678, 953) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2181) [M::mem_pestat] mean and std.dev: (762.36, 522.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2851) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 910.812 CPU sec, 40.470 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (81, 667140, 39, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 132, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 563) [M::mem_pestat] mean and std.dev: (125.53, 101.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 732) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.38, 75.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 48, 127) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 329) [M::mem_pestat] mean and std.dev: (57.52, 47.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 430) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (804, 1053, 1447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2733) [M::mem_pestat] mean and std.dev: (955.14, 396.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3376) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 902.590 CPU sec, 51.547 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (79, 667276, 25, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (44, 82, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (98.84, 75.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 660) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 262, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.34, 75.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (50, 91, 717) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2051) [M::mem_pestat] mean and std.dev: (203.14, 416.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2718) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 907.832 CPU sec, 40.310 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (90, 666620, 24, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (42, 54, 155) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 381) [M::mem_pestat] mean and std.dev: (73.56, 58.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 494) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.43, 75.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 77, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (75.95, 65.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 616) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 913.514 CPU sec, 51.821 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (71, 666523, 36, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (44, 75, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (92.87, 68.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 520) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.36, 75.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 151, 4257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12699) [M::mem_pestat] mean and std.dev: (1639.64, 2259.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16920) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (656, 819, 1703) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3797) [M::mem_pestat] mean and std.dev: (1047.50, 768.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4844) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 911.227 CPU sec, 53.405 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (72, 667316, 34, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (39, 68, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462) [M::mem_pestat] mean and std.dev: (79.43, 58.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.45, 75.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 64, 135) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 337) [M::mem_pestat] mean and std.dev: (61.23, 41.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 438) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 909.127 CPU sec, 40.358 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (84, 667122, 34, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (36, 85, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (91.84, 72.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 612) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.45, 75.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 57, 415) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1193) [M::mem_pestat] mean and std.dev: (128.00, 198.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1582) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 909.266 CPU sec, 53.177 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (64, 666711, 23, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (42, 75, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (95.23, 87.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 622) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.54, 75.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (38, 58, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 548) [M::mem_pestat] mean and std.dev: (86.37, 113.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 718) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (588, 972, 1504) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3336) [M::mem_pestat] mean and std.dev: (921.38, 495.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4252) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 912.486 CPU sec, 40.757 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (67, 666611, 27, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (36, 67, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 453) [M::mem_pestat] mean and std.dev: (99.56, 92.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 592) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.33, 75.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (53, 67, 925) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2669) [M::mem_pestat] mean and std.dev: (191.45, 328.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3541) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (652, 740, 976) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (4, 1624) [M::mem_pestat] mean and std.dev: (690.82, 304.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1948) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 914.507 CPU sec, 52.733 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (71, 667340, 31, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (42, 70, 144) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 348) [M::mem_pestat] mean and std.dev: (89.58, 63.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 450) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (216, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (12, 522) [M::mem_pestat] mean and std.dev: (269.37, 75.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 624) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (39, 103, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 429) [M::mem_pestat] mean and std.dev: (93.65, 83.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 559) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (631, 756, 1183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2287) [M::mem_pestat] mean and std.dev: (816.00, 405.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2839) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 906.318 CPU sec, 40.453 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (62, 667016, 23, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (45, 84, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (94.87, 67.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 525) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.26, 75.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (69, 959, 3890) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11532) [M::mem_pestat] mean and std.dev: (1747.26, 2152.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15353) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (500, 1172, 1381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3143) [M::mem_pestat] mean and std.dev: (779.20, 496.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4024) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 910.026 CPU sec, 54.489 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (72, 667017, 27, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (35, 70, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (95.18, 82.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 655) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.44, 75.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (47, 101, 746) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2144) [M::mem_pestat] mean and std.dev: (396.32, 599.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2843) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 907.094 CPU sec, 40.505 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (64, 666744, 26, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (43, 69, 154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 376) [M::mem_pestat] mean and std.dev: (80.48, 60.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 487) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.51, 75.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 115, 398) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1124) [M::mem_pestat] mean and std.dev: (156.32, 222.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1487) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (705, 942, 1862) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4176) [M::mem_pestat] mean and std.dev: (1090.24, 734.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5333) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 913.857 CPU sec, 53.824 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (83, 666913, 28, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (48, 66, 143) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 333) [M::mem_pestat] mean and std.dev: (92.50, 63.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 428) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.38, 75.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (54, 111, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378) [M::mem_pestat] mean and std.dev: (86.71, 54.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 486) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (557, 842, 1737) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4097) [M::mem_pestat] mean and std.dev: (1068.27, 834.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5277) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 904.750 CPU sec, 40.132 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (87, 666051, 39, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (44, 75, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (98.49, 86.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 600) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.50, 75.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 57, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616) [M::mem_pestat] mean and std.dev: (56.07, 49.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 810) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (630, 1092, 1585) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3495) [M::mem_pestat] mean and std.dev: (1136.08, 749.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4450) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 911.952 CPU sec, 53.487 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (79, 667514, 24, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (43, 78, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 433) [M::mem_pestat] mean and std.dev: (88.88, 63.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 563) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (11, 521) [M::mem_pestat] mean and std.dev: (269.22, 75.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 82, 1896) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5630) [M::mem_pestat] mean and std.dev: (862.61, 1355.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7497) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 895.387 CPU sec, 39.652 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (66, 667372, 30, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (39, 67, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 531) [M::mem_pestat] mean and std.dev: (102.59, 95.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 695) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.36, 75.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 54, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 440) [M::mem_pestat] mean and std.dev: (82.00, 92.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 579) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (536, 745, 1557) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3599) [M::mem_pestat] mean and std.dev: (940.27, 765.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4620) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 909.862 CPU sec, 53.173 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (75, 667426, 26, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (41, 64, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497) [M::mem_pestat] mean and std.dev: (96.34, 88.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 649) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.38, 75.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (46, 110, 643) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1837) [M::mem_pestat] mean and std.dev: (152.81, 259.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2434) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (787, 1672, 3120) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7786) [M::mem_pestat] mean and std.dev: (1928.27, 1427.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10119) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 905.324 CPU sec, 40.519 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (71, 666984, 26, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (51, 103, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 561) [M::mem_pestat] mean and std.dev: (109.20, 92.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 731) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (11, 521) [M::mem_pestat] mean and std.dev: (269.20, 75.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (40, 57, 120) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (65.65, 41.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 360) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 906.546 CPU sec, 50.639 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (95, 666815, 29, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (38, 73, 136) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 332) [M::mem_pestat] mean and std.dev: (79.07, 59.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 430) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.53, 75.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 63, 134) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 330) [M::mem_pestat] mean and std.dev: (74.12, 53.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 428) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1233, 1320, 4502) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11040) [M::mem_pestat] mean and std.dev: (2499.82, 2201.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14309) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 912.853 CPU sec, 52.972 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (81, 666750, 30, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (41, 66, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (88.49, 66.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 541) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.47, 75.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (38, 97, 1633) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4823) [M::mem_pestat] mean and std.dev: (915.76, 1517.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6984) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (575, 797, 2015) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4895) [M::mem_pestat] mean and std.dev: (1036.25, 702.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6335) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 907.296 CPU sec, 40.414 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (76, 666898, 27, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (44, 67, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (99.59, 83.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 604) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.30, 75.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (43, 195, 1737) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5125) [M::mem_pestat] mean and std.dev: (809.27, 1236.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6819) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (203, 1144, 2596) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7382) [M::mem_pestat] mean and std.dev: (1597.69, 1731.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9775) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 912.401 CPU sec, 53.739 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (78, 666819, 27, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (37, 57, 152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 382) [M::mem_pestat] mean and std.dev: (83.76, 64.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 497) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.39, 75.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (54, 117, 380) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1032) [M::mem_pestat] mean and std.dev: (98.19, 85.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1358) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (784, 1121, 1942) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4258) [M::mem_pestat] mean and std.dev: (1237.25, 996.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5416) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 908.855 CPU sec, 40.505 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (73, 666925, 19, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (36, 61, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 528) [M::mem_pestat] mean and std.dev: (93.78, 99.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 692) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.42, 75.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 51, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 465) [M::mem_pestat] mean and std.dev: (63.19, 56.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 613) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (473, 1192, 3548) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9698) [M::mem_pestat] mean and std.dev: (1739.08, 1522.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12773) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 910.874 CPU sec, 53.928 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (69, 667190, 31, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (36, 63, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (78.61, 59.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 540) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.43, 75.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 84, 688) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2002) [M::mem_pestat] mean and std.dev: (270.75, 439.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2659) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (185, 1583, 2052) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5786) [M::mem_pestat] mean and std.dev: (1479.55, 1376.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7653) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 905.936 CPU sec, 40.293 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (75, 666637, 29, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (50, 114, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (109.35, 81.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.39, 75.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (42, 126, 3246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9654) [M::mem_pestat] mean and std.dev: (1245.14, 2103.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12858) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 908.470 CPU sec, 53.534 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (68, 666795, 33, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (37, 58, 106) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 244) [M::mem_pestat] mean and std.dev: (67.10, 45.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 313) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.36, 75.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (43, 105, 3785) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11269) [M::mem_pestat] mean and std.dev: (1559.48, 2349.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15011) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (458, 851, 1496) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3572) [M::mem_pestat] mean and std.dev: (1097.50, 796.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4610) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 914.763 CPU sec, 40.720 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (75, 666951, 31, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (49, 62, 152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 358) [M::mem_pestat] mean and std.dev: (82.31, 55.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.24, 75.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 62, 152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388) [M::mem_pestat] mean and std.dev: (57.79, 40.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 506) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (208, 910, 2624) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7456) [M::mem_pestat] mean and std.dev: (1567.12, 1774.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9872) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 907.786 CPU sec, 51.596 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (73, 666902, 29, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (38, 74, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (82.85, 68.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.42, 75.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (49, 108, 1642) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4828) [M::mem_pestat] mean and std.dev: (897.11, 1580.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7221) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (810, 1187, 1678) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3414) [M::mem_pestat] mean and std.dev: (1064.33, 482.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4282) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 903.551 CPU sec, 40.193 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (64, 667034, 29, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (38, 71, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 485) [M::mem_pestat] mean and std.dev: (88.88, 77.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 634) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.42, 75.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 107, 1681) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4971) [M::mem_pestat] mean and std.dev: (984.34, 1599.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7381) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 908.038 CPU sec, 53.399 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (66, 667364, 29, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (40, 100, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 541) [M::mem_pestat] mean and std.dev: (109.29, 88.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 708) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 262, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.29, 75.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 94, 159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 413) [M::mem_pestat] mean and std.dev: (74.67, 52.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 540) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1006, 1770, 2758) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6262) [M::mem_pestat] mean and std.dev: (1895.50, 1253.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8014) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 901.873 CPU sec, 40.028 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (73, 667284, 23, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (39, 92, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585) [M::mem_pestat] mean and std.dev: (117.40, 119.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 767) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.39, 75.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (56, 139, 1451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4241) [M::mem_pestat] mean and std.dev: (254.28, 399.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5636) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (340, 828, 1322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3286) [M::mem_pestat] mean and std.dev: (901.64, 738.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4268) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 907.380 CPU sec, 53.080 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (78, 666577, 38, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (39, 82, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 537) [M::mem_pestat] mean and std.dev: (109.86, 98.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 703) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.48, 75.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 55, 472) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1364) [M::mem_pestat] mean and std.dev: (148.62, 264.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1810) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (747, 1065, 1683) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3555) [M::mem_pestat] mean and std.dev: (1243.23, 764.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4491) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 908.846 CPU sec, 40.445 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (90, 666534, 17, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (45, 76, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 456) [M::mem_pestat] mean and std.dev: (101.05, 85.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.40, 75.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 144, 1291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3805) [M::mem_pestat] mean and std.dev: (495.71, 784.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5062) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 913.268 CPU sec, 47.642 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (75, 666353, 27, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (47, 73, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 515) [M::mem_pestat] mean and std.dev: (95.66, 70.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 671) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.49, 75.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 79, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645) [M::mem_pestat] mean and std.dev: (79.50, 69.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 848) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 912.813 CPU sec, 54.827 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (71, 666602, 31, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (37, 63, 142) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 352) [M::mem_pestat] mean and std.dev: (79.61, 65.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 457) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.39, 75.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 75, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513) [M::mem_pestat] mean and std.dev: (65.29, 52.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 676) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (541, 1309, 2626) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6796) [M::mem_pestat] mean and std.dev: (1414.33, 1073.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8881) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 912.556 CPU sec, 40.685 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (86, 666051, 32, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (39, 72, 153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 381) [M::mem_pestat] mean and std.dev: (87.56, 67.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 495) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.30, 75.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (41, 117, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1205) [M::mem_pestat] mean and std.dev: (126.96, 149.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1593) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1146, 1503, 2729) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5895) [M::mem_pestat] mean and std.dev: (1703.23, 945.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7478) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 919.526 CPU sec, 54.576 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (87, 667111, 26, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (49, 93, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (105.66, 83.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 545) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (11, 521) [M::mem_pestat] mean and std.dev: (269.16, 75.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 71, 571) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1681) [M::mem_pestat] mean and std.dev: (74.20, 119.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2236) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (274, 786, 1226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3130) [M::mem_pestat] mean and std.dev: (726.64, 534.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4082) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 909.879 CPU sec, 40.371 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (72, 667002, 32, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 98, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 501) [M::mem_pestat] mean and std.dev: (112.80, 89.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 650) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.27, 75.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 119, 2687) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7997) [M::mem_pestat] mean and std.dev: (1155.84, 1769.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10652) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (729, 897, 1234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2244) [M::mem_pestat] mean and std.dev: (858.73, 393.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2749) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 913.096 CPU sec, 53.507 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (64, 666575, 39, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (39, 68, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 504) [M::mem_pestat] mean and std.dev: (103.73, 88.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 659) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.41, 75.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (42, 122, 1303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3825) [M::mem_pestat] mean and std.dev: (390.79, 678.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5086) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (171, 783, 1102) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2964) [M::mem_pestat] mean and std.dev: (792.88, 612.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3895) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 909.617 CPU sec, 40.289 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (76, 666941, 29, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (41, 73, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 545) [M::mem_pestat] mean and std.dev: (111.32, 114.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 713) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (11, 521) [M::mem_pestat] mean and std.dev: (269.19, 75.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (46, 76, 799) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2305) [M::mem_pestat] mean and std.dev: (199.60, 308.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3058) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (558, 829, 1105) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2199) [M::mem_pestat] mean and std.dev: (708.75, 377.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2746) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 909.176 CPU sec, 53.468 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (76, 666649, 26, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (46, 78, 151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 361) [M::mem_pestat] mean and std.dev: (88.86, 59.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 466) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.24, 75.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 149, 2443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7259) [M::mem_pestat] mean and std.dev: (1052.67, 1533.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9667) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (657, 973, 1334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2688) [M::mem_pestat] mean and std.dev: (964.85, 452.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3365) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 909.597 CPU sec, 40.526 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (69, 666999, 39, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (38, 73, 130) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 314) [M::mem_pestat] mean and std.dev: (89.67, 70.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 406) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.25, 75.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 50, 138) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 370) [M::mem_pestat] mean and std.dev: (53.24, 48.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 486) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (575, 744, 1574) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3572) [M::mem_pestat] mean and std.dev: (944.90, 597.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4571) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 910.138 CPU sec, 52.408 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (74, 666426, 40, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (39, 70, 153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 381) [M::mem_pestat] mean and std.dev: (90.48, 77.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 495) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.42, 75.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 81, 552) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1608) [M::mem_pestat] mean and std.dev: (130.22, 249.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2136) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (678, 1076, 1331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2637) [M::mem_pestat] mean and std.dev: (943.22, 655.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3564) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 908.147 CPU sec, 40.315 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (63, 666906, 28, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (44, 73, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 404) [M::mem_pestat] mean and std.dev: (78.06, 50.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 524) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 262, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.20, 75.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 61, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 508) [M::mem_pestat] mean and std.dev: (66.00, 47.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 665) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (701, 1096, 1820) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4058) [M::mem_pestat] mean and std.dev: (1202.00, 758.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5177) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 911.798 CPU sec, 53.127 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (75, 667331, 29, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (44, 85, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 422) [M::mem_pestat] mean and std.dev: (106.96, 84.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 548) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.53, 75.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (40, 117, 1580) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4660) [M::mem_pestat] mean and std.dev: (685.50, 1127.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6200) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (867, 1105, 1385) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2421) [M::mem_pestat] mean and std.dev: (1146.77, 367.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2939) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 906.458 CPU sec, 40.164 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (59, 667077, 23, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (50, 100, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497) [M::mem_pestat] mean and std.dev: (98.69, 75.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 646) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.40, 75.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 82, 993) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2911) [M::mem_pestat] mean and std.dev: (191.00, 312.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3870) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (820, 1290, 1966) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4258) [M::mem_pestat] mean and std.dev: (1426.14, 826.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5404) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 908.964 CPU sec, 52.649 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (76, 667034, 33, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (42, 64, 147) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 357) [M::mem_pestat] mean and std.dev: (90.21, 63.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.40, 75.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (55, 109, 811) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2323) [M::mem_pestat] mean and std.dev: (208.11, 286.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3079) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 908.145 CPU sec, 40.333 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (81, 666972, 29, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (50, 108, 510) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1430) [M::mem_pestat] mean and std.dev: (279.71, 370.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1890) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.48, 75.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (44, 86, 2392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7088) [M::mem_pestat] mean and std.dev: (772.23, 1384.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9436) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (258, 675, 1410) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3714) [M::mem_pestat] mean and std.dev: (791.26, 634.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4866) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 898.811 CPU sec, 44.708 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (91, 666954, 27, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (41, 62, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407) [M::mem_pestat] mean and std.dev: (82.86, 67.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 529) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.30, 75.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (47, 104, 145) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 341) [M::mem_pestat] mean and std.dev: (97.16, 71.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 439) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1030, 1435, 2121) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4303) [M::mem_pestat] mean and std.dev: (1368.30, 579.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5394) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 910.437 CPU sec, 53.206 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (88, 666786, 28, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (39, 66, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (96.54, 85.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 531) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.49, 75.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 97, 768) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2238) [M::mem_pestat] mean and std.dev: (266.20, 485.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2973) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (481, 657, 1118) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2392) [M::mem_pestat] mean and std.dev: (678.25, 408.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3029) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 904.578 CPU sec, 40.081 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (81, 666727, 30, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (39, 64, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 432) [M::mem_pestat] mean and std.dev: (96.65, 79.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 563) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.36, 75.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (40, 77, 133) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 319) [M::mem_pestat] mean and std.dev: (73.12, 47.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 412) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (720, 1121, 1859) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4137) [M::mem_pestat] mean and std.dev: (1356.79, 977.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5276) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 909.548 CPU sec, 51.936 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (76, 667303, 26, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (46, 74, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 445) [M::mem_pestat] mean and std.dev: (96.29, 78.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.34, 75.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 119, 943) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2779) [M::mem_pestat] mean and std.dev: (347.00, 641.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3697) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (445, 827, 1413) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3349) [M::mem_pestat] mean and std.dev: (897.25, 715.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4317) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 903.808 CPU sec, 40.180 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (81, 666464, 18, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (48, 99, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (99.62, 77.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 632) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.47, 75.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (74, 148, 553) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1511) [M::mem_pestat] mean and std.dev: (224.07, 275.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1990) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (556, 764, 1070) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2098) [M::mem_pestat] mean and std.dev: (688.79, 312.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2612) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 912.335 CPU sec, 52.567 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (65, 667012, 29, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (48, 105, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 498) [M::mem_pestat] mean and std.dev: (114.54, 86.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 648) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (11, 521) [M::mem_pestat] mean and std.dev: (269.14, 75.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (44, 79, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 437) [M::mem_pestat] mean and std.dev: (84.08, 73.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 568) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (205, 880, 1889) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5257) [M::mem_pestat] mean and std.dev: (1034.15, 936.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6941) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 904.733 CPU sec, 40.364 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (62, 666849, 34, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (39, 65, 156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 390) [M::mem_pestat] mean and std.dev: (91.68, 75.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 507) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.43, 75.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 104, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1247) [M::mem_pestat] mean and std.dev: (100.30, 149.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1653) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (582, 1312, 2436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6144) [M::mem_pestat] mean and std.dev: (1369.28, 1170.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7998) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 913.274 CPU sec, 53.678 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (86, 667558, 35, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (43, 64, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 397) [M::mem_pestat] mean and std.dev: (79.93, 61.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 515) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.49, 75.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 70, 1364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4030) [M::mem_pestat] mean and std.dev: (499.19, 961.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5363) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (243, 770, 1035) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2619) [M::mem_pestat] mean and std.dev: (632.14, 423.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3411) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 908.657 CPU sec, 40.440 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (78, 666749, 24, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (37, 65, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (88.22, 71.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 549) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.37, 75.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (39, 87, 1603) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4731) [M::mem_pestat] mean and std.dev: (837.91, 1470.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6722) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (869, 1780, 2297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5153) [M::mem_pestat] mean and std.dev: (1542.40, 980.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6581) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 910.945 CPU sec, 53.183 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (85, 666671, 33, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (46, 96, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (102.00, 73.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 634) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.40, 75.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (40, 157, 1624) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4792) [M::mem_pestat] mean and std.dev: (905.50, 1439.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6665) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (787, 1004, 2024) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4498) [M::mem_pestat] mean and std.dev: (1293.47, 715.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5735) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 909.412 CPU sec, 40.394 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (69, 666808, 36, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (45, 89, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (104.10, 93.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 677) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.44, 75.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 59, 719) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2111) [M::mem_pestat] mean and std.dev: (203.42, 433.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2807) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (199, 838, 1536) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4210) [M::mem_pestat] mean and std.dev: (994.82, 883.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5547) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 913.057 CPU sec, 53.809 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (69, 667444, 31, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (42, 103, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 633) [M::mem_pestat] mean and std.dev: (120.48, 112.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 830) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.42, 75.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 116, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 727) [M::mem_pestat] mean and std.dev: (121.81, 138.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 957) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (625, 1466, 3295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8635) [M::mem_pestat] mean and std.dev: (1959.44, 1910.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11305) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 907.374 CPU sec, 40.505 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (69, 666835, 25, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (40, 77, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 433) [M::mem_pestat] mean and std.dev: (96.27, 80.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 564) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.40, 75.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 118, 690) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2012) [M::mem_pestat] mean and std.dev: (211.67, 385.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2673) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (297, 1339, 1714) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4548) [M::mem_pestat] mean and std.dev: (1036.50, 761.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5965) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 910.441 CPU sec, 54.035 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (71, 666961, 49, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (40, 65, 134) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 322) [M::mem_pestat] mean and std.dev: (82.20, 61.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 416) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 262, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.30, 75.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 85, 1196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3516) [M::mem_pestat] mean and std.dev: (402.53, 756.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4676) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (592, 889, 1765) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4111) [M::mem_pestat] mean and std.dev: (1058.00, 697.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5284) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 906.433 CPU sec, 40.520 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (88, 667033, 30, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (42, 76, 145) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 351) [M::mem_pestat] mean and std.dev: (86.64, 60.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 454) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.38, 75.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (38, 121, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 566) [M::mem_pestat] mean and std.dev: (105.28, 113.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 742) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (724, 918, 1180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2092) [M::mem_pestat] mean and std.dev: (736.00, 370.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2548) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 901.549 CPU sec, 41.623 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (78, 667458, 20, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (37, 67, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 439) [M::mem_pestat] mean and std.dev: (88.87, 70.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.30, 75.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 140, 1764) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5222) [M::mem_pestat] mean and std.dev: (869.40, 1149.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6951) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (641, 1219, 2172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5234) [M::mem_pestat] mean and std.dev: (1179.67, 798.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6765) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 909.020 CPU sec, 53.175 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (74, 666882, 45, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (37, 80, 145) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 361) [M::mem_pestat] mean and std.dev: (95.24, 74.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 469) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.35, 75.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 96, 1098) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3228) [M::mem_pestat] mean and std.dev: (339.15, 583.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4293) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (595, 839, 1893) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4489) [M::mem_pestat] mean and std.dev: (1089.59, 884.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5787) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 903.986 CPU sec, 40.140 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (71, 667326, 31, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (43, 75, 138) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 328) [M::mem_pestat] mean and std.dev: (94.65, 68.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 423) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (216, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (12, 522) [M::mem_pestat] mean and std.dev: (269.50, 75.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 624) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (48, 103, 1892) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5580) [M::mem_pestat] mean and std.dev: (1025.53, 1714.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7884) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (395, 1018, 1741) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4433) [M::mem_pestat] mean and std.dev: (1161.18, 1070.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5779) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 903.525 CPU sec, 51.845 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (77, 666685, 22, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (36, 67, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 453) [M::mem_pestat] mean and std.dev: (95.89, 81.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 592) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.43, 75.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (9, 41, 109) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 309) [M::mem_pestat] mean and std.dev: (37.89, 34.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 409) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 907.613 CPU sec, 40.522 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (73, 666864, 20, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (38, 77, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 584) [M::mem_pestat] mean and std.dev: (90.25, 71.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 766) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.29, 75.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (47, 107, 1441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4229) [M::mem_pestat] mean and std.dev: (570.63, 972.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5623) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (323, 923, 2090) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5624) [M::mem_pestat] mean and std.dev: (953.50, 811.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7391) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 910.149 CPU sec, 53.844 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (81, 666759, 33, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (43, 62, 138) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 328) [M::mem_pestat] mean and std.dev: (80.65, 57.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 423) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.42, 75.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (48, 100, 2006) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5922) [M::mem_pestat] mean and std.dev: (711.17, 1354.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7880) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 911.535 CPU sec, 40.805 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (70, 666893, 25, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (43, 73, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424) [M::mem_pestat] mean and std.dev: (99.31, 82.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 551) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.47, 75.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 81, 2520) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7486) [M::mem_pestat] mean and std.dev: (1059.38, 1653.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9969) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 906.416 CPU sec, 53.055 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (80, 666870, 23, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (40, 73, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 523) [M::mem_pestat] mean and std.dev: (97.37, 88.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 684) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.49, 75.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 54, 128) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 344) [M::mem_pestat] mean and std.dev: (59.80, 57.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 452) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (930, 964, 2632) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6036) [M::mem_pestat] mean and std.dev: (1485.91, 1075.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7738) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 908.062 CPU sec, 40.328 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (80, 667099, 26, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (45, 78, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723) [M::mem_pestat] mean and std.dev: (121.04, 141.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 949) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.33, 75.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 142, 1207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3547) [M::mem_pestat] mean and std.dev: (462.04, 873.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4717) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (590, 1171, 3026) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7898) [M::mem_pestat] mean and std.dev: (1607.31, 1219.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10334) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 910.737 CPU sec, 53.174 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (62, 667072, 25, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (44, 74, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407) [M::mem_pestat] mean and std.dev: (90.44, 74.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 528) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.38, 75.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (44, 148, 2580) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7652) [M::mem_pestat] mean and std.dev: (1118.40, 1645.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10188) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 912.410 CPU sec, 40.755 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (72, 667371, 21, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (37, 79, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (95.97, 71.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 533) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.41, 75.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 66, 2425) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7229) [M::mem_pestat] mean and std.dev: (1088.71, 1871.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9631) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (691, 826, 2430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5908) [M::mem_pestat] mean and std.dev: (1224.00, 991.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7647) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 908.447 CPU sec, 53.773 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (88, 667461, 25, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (44, 74, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497) [M::mem_pestat] mean and std.dev: (106.85, 99.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 648) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.42, 75.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 61, 475) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1371) [M::mem_pestat] mean and std.dev: (80.90, 106.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1819) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 904.924 CPU sec, 40.343 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (77, 666794, 32, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (43, 70, 146) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 352) [M::mem_pestat] mean and std.dev: (90.41, 71.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 455) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.29, 75.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 109, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 563) [M::mem_pestat] mean and std.dev: (78.68, 60.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 739) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (569, 1096, 4231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11555) [M::mem_pestat] mean and std.dev: (2040.38, 1921.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15217) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 913.129 CPU sec, 54.704 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 666447, 13, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (47, 102, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 545) [M::mem_pestat] mean and std.dev: (116.07, 97.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 711) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 262, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (11, 521) [M::mem_pestat] mean and std.dev: (269.16, 75.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 84, 1949) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5789) [M::mem_pestat] mean and std.dev: (756.08, 1145.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7709) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (631, 696, 1231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2431) [M::mem_pestat] mean and std.dev: (811.07, 476.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3031) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 908.282 CPU sec, 40.623 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (70, 666655, 27, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (45, 71, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 444) [M::mem_pestat] mean and std.dev: (103.05, 85.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 577) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.29, 75.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (56, 137, 376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1016) [M::mem_pestat] mean and std.dev: (149.18, 174.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1336) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (431, 781, 1521) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3701) [M::mem_pestat] mean and std.dev: (1040.39, 885.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4791) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 910.897 CPU sec, 53.416 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (73, 666820, 42, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (37, 62, 150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 376) [M::mem_pestat] mean and std.dev: (80.55, 75.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 489) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.32, 75.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (38, 80, 144) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 356) [M::mem_pestat] mean and std.dev: (75.43, 67.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 913.830 CPU sec, 40.811 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (66, 666321, 22, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (42, 83, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 555) [M::mem_pestat] mean and std.dev: (100.05, 82.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 726) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.40, 75.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 110, 1563) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4619) [M::mem_pestat] mean and std.dev: (835.52, 1439.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6592) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (697, 940, 1988) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4570) [M::mem_pestat] mean and std.dev: (1146.83, 847.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5861) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 910.644 CPU sec, 53.842 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (87, 666611, 29, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (40, 65, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 511) [M::mem_pestat] mean and std.dev: (85.72, 68.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 668) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.37, 75.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (44, 88, 639) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1829) [M::mem_pestat] mean and std.dev: (257.88, 378.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2424) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (226, 1462, 2502) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7054) [M::mem_pestat] mean and std.dev: (1490.57, 1524.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9330) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 908.534 CPU sec, 40.579 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (79, 666545, 36, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (37, 93, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 535) [M::mem_pestat] mean and std.dev: (105.46, 92.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 701) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.32, 75.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (40, 107, 1869) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5527) [M::mem_pestat] mean and std.dev: (1061.47, 1761.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8109) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 915.797 CPU sec, 53.816 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (71, 666801, 25, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (40, 67, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 517) [M::mem_pestat] mean and std.dev: (97.90, 94.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 676) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.28, 75.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (74, 136, 1863) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5441) [M::mem_pestat] mean and std.dev: (849.76, 1392.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7230) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (806, 1312, 2505) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5903) [M::mem_pestat] mean and std.dev: (1720.38, 1621.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8208) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 908.509 CPU sec, 40.397 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (69, 667061, 23, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (34, 60, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (77.18, 63.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 618) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.39, 75.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 83, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 446) [M::mem_pestat] mean and std.dev: (57.67, 47.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (322, 777, 1432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3652) [M::mem_pestat] mean and std.dev: (910.19, 872.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4762) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 910.380 CPU sec, 53.362 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (70, 667068, 26, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (44, 86, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (104.57, 90.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 660) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.55, 75.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 104, 843) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2457) [M::mem_pestat] mean and std.dev: (294.35, 489.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3264) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (363, 1051, 1772) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4590) [M::mem_pestat] mean and std.dev: (1376.71, 1279.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6494) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 910.354 CPU sec, 40.387 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (82, 666538, 21, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (36, 62, 132) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 324) [M::mem_pestat] mean and std.dev: (76.51, 58.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 420) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.56, 75.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 44, 1904) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5656) [M::mem_pestat] mean and std.dev: (874.10, 1444.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7532) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (258, 679, 1243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3213) [M::mem_pestat] mean and std.dev: (683.88, 472.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4198) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 909.928 CPU sec, 52.375 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (64, 666560, 22, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (48, 113, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 522) [M::mem_pestat] mean and std.dev: (116.49, 99.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 680) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.33, 75.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 99, 823) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2411) [M::mem_pestat] mean and std.dev: (369.10, 636.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3205) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (670, 1027, 1461) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3043) [M::mem_pestat] mean and std.dev: (1060.19, 658.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3834) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 912.142 CPU sec, 40.451 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (75, 667280, 26, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (49, 81, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 427) [M::mem_pestat] mean and std.dev: (105.50, 76.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 553) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.44, 75.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (41, 112, 2687) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7979) [M::mem_pestat] mean and std.dev: (1342.08, 1862.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10625) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (760, 846, 1526) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3058) [M::mem_pestat] mean and std.dev: (881.00, 632.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3824) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 912.967 CPU sec, 54.164 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (59, 666770, 38, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (43, 78, 152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 370) [M::mem_pestat] mean and std.dev: (99.86, 79.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 479) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.32, 75.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 88, 371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1041) [M::mem_pestat] mean and std.dev: (71.38, 70.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1376) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (925, 1078, 1988) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4114) [M::mem_pestat] mean and std.dev: (1349.57, 688.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5177) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 909.212 CPU sec, 40.619 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (89, 666395, 23, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (42, 68, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648) [M::mem_pestat] mean and std.dev: (105.97, 119.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 850) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.45, 75.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (46, 107, 1356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3976) [M::mem_pestat] mean and std.dev: (297.79, 612.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5286) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (382, 808, 933) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2035) [M::mem_pestat] mean and std.dev: (663.90, 390.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2586) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 911.580 CPU sec, 53.542 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (81, 667257, 26, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (32, 59, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 443) [M::mem_pestat] mean and std.dev: (84.08, 72.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 580) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.42, 75.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 82, 150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 384) [M::mem_pestat] mean and std.dev: (71.67, 51.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 501) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (723, 935, 2148) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4998) [M::mem_pestat] mean and std.dev: (1403.23, 1088.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6423) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 904.208 CPU sec, 40.315 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (72, 666655, 37, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (40, 90, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 427) [M::mem_pestat] mean and std.dev: (99.69, 78.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 556) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (216, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (12, 522) [M::mem_pestat] mean and std.dev: (269.46, 75.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 624) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 56, 877) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2585) [M::mem_pestat] mean and std.dev: (104.28, 200.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3439) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1093, 1610, 2166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4312) [M::mem_pestat] mean and std.dev: (1232.12, 676.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5385) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 915.551 CPU sec, 53.476 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (72, 667106, 20, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (42, 85, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 465) [M::mem_pestat] mean and std.dev: (94.41, 75.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.41, 75.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 35, 153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 413) [M::mem_pestat] mean and std.dev: (45.69, 45.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 543) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (704, 1877, 2574) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6314) [M::mem_pestat] mean and std.dev: (1733.07, 1318.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8184) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 909.481 CPU sec, 51.539 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (73, 666866, 29, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (42, 74, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (94.21, 70.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 530) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.41, 75.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 77, 560) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1620) [M::mem_pestat] mean and std.dev: (122.62, 241.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2150) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (833, 1941, 2467) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5735) [M::mem_pestat] mean and std.dev: (1788.90, 1218.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7369) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 910.729 CPU sec, 40.516 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (77, 667206, 22, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (35, 56, 128) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 314) [M::mem_pestat] mean and std.dev: (70.20, 51.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 407) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (216, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (12, 522) [M::mem_pestat] mean and std.dev: (269.58, 75.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 624) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (55, 666, 4343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12919) [M::mem_pestat] mean and std.dev: (1666.73, 2031.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17207) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (504, 820, 1656) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3960) [M::mem_pestat] mean and std.dev: (1129.64, 1002.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5138) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 910.560 CPU sec, 53.976 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (78, 667242, 24, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (45, 88, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (92.97, 67.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 625) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.25, 75.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (45, 65, 128) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 294) [M::mem_pestat] mean and std.dev: (82.50, 62.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 377) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (527, 849, 1810) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4376) [M::mem_pestat] mean and std.dev: (998.50, 621.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5659) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 909.349 CPU sec, 40.510 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (71, 667145, 24, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (47, 84, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 440) [M::mem_pestat] mean and std.dev: (96.67, 75.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 571) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.44, 75.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 87, 1396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4144) [M::mem_pestat] mean and std.dev: (481.73, 812.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5518) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (972, 1518, 2468) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5460) [M::mem_pestat] mean and std.dev: (1459.64, 721.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6956) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 909.603 CPU sec, 53.501 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (66, 667426, 29, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (42, 65, 121) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 279) [M::mem_pestat] mean and std.dev: (80.89, 60.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 358) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.41, 75.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (54, 78, 1119) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3249) [M::mem_pestat] mean and std.dev: (390.54, 742.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4314) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (859, 1171, 1869) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3889) [M::mem_pestat] mean and std.dev: (1257.73, 763.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4899) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 911.535 CPU sec, 40.845 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (62, 667574, 29, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (48, 68, 141) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 327) [M::mem_pestat] mean and std.dev: (93.93, 65.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 420) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.45, 75.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (38, 123, 1598) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4718) [M::mem_pestat] mean and std.dev: (890.14, 1450.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6690) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 906.671 CPU sec, 51.966 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (74, 667164, 32, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (39, 77, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 441) [M::mem_pestat] mean and std.dev: (96.47, 82.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 575) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.32, 75.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (68, 129, 870) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2474) [M::mem_pestat] mean and std.dev: (196.81, 347.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3276) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (481, 714, 847) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1579) [M::mem_pestat] mean and std.dev: (536.73, 310.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1945) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 901.649 CPU sec, 39.855 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (86, 666609, 34, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (44, 71, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 437) [M::mem_pestat] mean and std.dev: (87.01, 76.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 568) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.44, 75.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (59, 147, 972) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2798) [M::mem_pestat] mean and std.dev: (269.14, 450.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3711) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (812, 1420, 2647) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6317) [M::mem_pestat] mean and std.dev: (1925.41, 1761.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8973) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 914.689 CPU sec, 54.343 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (94, 667085, 29, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (38, 73, 144) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 356) [M::mem_pestat] mean and std.dev: (85.01, 63.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 262, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.36, 75.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (40, 107, 1342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3946) [M::mem_pestat] mean and std.dev: (306.38, 560.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5248) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 905.636 CPU sec, 40.167 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (64, 666889, 22, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (38, 61, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 422) [M::mem_pestat] mean and std.dev: (92.78, 78.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 550) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.44, 75.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 90, 149) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 391) [M::mem_pestat] mean and std.dev: (67.28, 48.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 512) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (385, 702, 1334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3232) [M::mem_pestat] mean and std.dev: (846.80, 709.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4181) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 904.053 CPU sec, 52.622 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (63, 666826, 29, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (43, 77, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460) [M::mem_pestat] mean and std.dev: (100.88, 87.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 599) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.50, 75.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 86, 1340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3960) [M::mem_pestat] mean and std.dev: (498.54, 896.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5270) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 906.759 CPU sec, 40.610 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (81, 667092, 31, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (48, 107, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570) [M::mem_pestat] mean and std.dev: (116.27, 95.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 744) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.30, 75.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 55, 1381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4101) [M::mem_pestat] mean and std.dev: (414.07, 731.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5461) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (662, 927, 1657) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3647) [M::mem_pestat] mean and std.dev: (1066.76, 706.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4642) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 911.483 CPU sec, 53.845 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (80, 666942, 33, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (49, 78, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 511) [M::mem_pestat] mean and std.dev: (99.83, 76.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 665) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.48, 75.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 71, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570) [M::mem_pestat] mean and std.dev: (66.62, 49.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 749) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (834, 1474, 1896) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4020) [M::mem_pestat] mean and std.dev: (1214.10, 598.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5082) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 912.994 CPU sec, 40.792 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (83, 667089, 15, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (38, 68, 126) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 302) [M::mem_pestat] mean and std.dev: (91.91, 74.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 391) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.40, 75.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (60, 72, 143) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 309) [M::mem_pestat] mean and std.dev: (76.67, 35.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 392) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (281, 837, 1071) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2651) [M::mem_pestat] mean and std.dev: (749.33, 535.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3441) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 906.359 CPU sec, 52.996 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (67, 666631, 29, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (42, 72, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 543) [M::mem_pestat] mean and std.dev: (115.39, 117.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 710) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.40, 75.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 66, 1514) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4492) [M::mem_pestat] mean and std.dev: (416.81, 761.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5981) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 913.965 CPU sec, 53.095 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (71, 667161, 26, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (39, 64, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 453) [M::mem_pestat] mean and std.dev: (95.08, 75.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 591) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.53, 75.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (51, 109, 806) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2316) [M::mem_pestat] mean and std.dev: (173.85, 235.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3071) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (762, 999, 1925) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4251) [M::mem_pestat] mean and std.dev: (1282.20, 714.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5414) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 902.770 CPU sec, 39.953 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (82, 667004, 25, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (34, 66, 151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 385) [M::mem_pestat] mean and std.dev: (77.96, 56.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 502) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.47, 75.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 109, 1982) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5882) [M::mem_pestat] mean and std.dev: (940.79, 1795.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8122) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 903.863 CPU sec, 52.692 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (68, 666100, 26, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (37, 70, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 433) [M::mem_pestat] mean and std.dev: (77.50, 57.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 565) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.39, 75.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 112, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (89.33, 106.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 652) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 909.567 CPU sec, 40.475 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (75, 666818, 29, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (45, 80, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 624) [M::mem_pestat] mean and std.dev: (112.41, 110.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 817) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.47, 75.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 109, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (80.58, 64.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 621) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (432, 790, 1141) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2559) [M::mem_pestat] mean and std.dev: (769.12, 491.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3268) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 901.464 CPU sec, 50.663 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (87, 667225, 32, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (38, 60, 154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 386) [M::mem_pestat] mean and std.dev: (82.26, 60.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 502) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.33, 75.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (47, 93, 551) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1559) [M::mem_pestat] mean and std.dev: (249.21, 428.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2063) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (691, 1261, 2433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5917) [M::mem_pestat] mean and std.dev: (1339.67, 924.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7659) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 903.579 CPU sec, 40.266 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (92, 667130, 28, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (40, 68, 127) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 301) [M::mem_pestat] mean and std.dev: (78.49, 53.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 388) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.32, 75.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 56, 142) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 362) [M::mem_pestat] mean and std.dev: (68.58, 55.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 472) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 903.159 CPU sec, 52.457 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (77, 666651, 24, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (43, 100, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 718) [M::mem_pestat] mean and std.dev: (110.84, 105.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 943) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.56, 75.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (95, 145, 1027) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2891) [M::mem_pestat] mean and std.dev: (480.82, 808.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3823) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (261, 753, 2411) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6711) [M::mem_pestat] mean and std.dev: (1127.08, 1307.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8861) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 903.190 CPU sec, 40.049 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (63, 666907, 33, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (39, 60, 142) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 348) [M::mem_pestat] mean and std.dev: (87.90, 75.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 451) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.40, 75.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (44, 94, 858) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2486) [M::mem_pestat] mean and std.dev: (223.04, 492.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3300) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 905.875 CPU sec, 52.623 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (75, 667206, 22, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (49, 96, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (106.95, 97.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 649) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 262, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.22, 75.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 95, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 545) [M::mem_pestat] mean and std.dev: (99.78, 111.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 716) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (753, 1049, 1756) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3762) [M::mem_pestat] mean and std.dev: (1286.00, 1012.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5336) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 902.796 CPU sec, 40.055 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (79, 666853, 30, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (42, 79, 143) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 345) [M::mem_pestat] mean and std.dev: (91.41, 65.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 446) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 262, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (11, 521) [M::mem_pestat] mean and std.dev: (269.12, 75.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 83, 1234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3636) [M::mem_pestat] mean and std.dev: (493.86, 895.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4837) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (477, 997, 1536) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3654) [M::mem_pestat] mean and std.dev: (977.29, 708.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4713) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 903.394 CPU sec, 52.273 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (73, 666737, 28, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (33, 70, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 456) [M::mem_pestat] mean and std.dev: (85.71, 68.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.29, 75.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (55, 97, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 376) [M::mem_pestat] mean and std.dev: (86.09, 56.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 483) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (627, 997, 1487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3207) [M::mem_pestat] mean and std.dev: (981.90, 676.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4067) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 902.456 CPU sec, 40.013 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (66, 666660, 33, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (42, 78, 155) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 381) [M::mem_pestat] mean and std.dev: (81.96, 61.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 494) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.33, 75.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 65, 459) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1319) [M::mem_pestat] mean and std.dev: (145.00, 240.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1749) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (635, 1253, 1671) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3743) [M::mem_pestat] mean and std.dev: (1125.31, 676.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4779) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 904.123 CPU sec, 52.351 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (77, 666647, 27, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (32, 61, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 434) [M::mem_pestat] mean and std.dev: (88.07, 85.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 568) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.39, 75.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 153, 1780) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5270) [M::mem_pestat] mean and std.dev: (820.52, 1242.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7015) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (807, 1135, 3093) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7665) [M::mem_pestat] mean and std.dev: (1649.92, 1215.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9951) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 904.429 CPU sec, 40.280 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (67, 667203, 25, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (36, 74, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (85.43, 74.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 624) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.40, 75.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 120, 715) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2099) [M::mem_pestat] mean and std.dev: (215.14, 325.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2791) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 904.353 CPU sec, 52.957 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (74, 666202, 40, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (41, 78, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 611) [M::mem_pestat] mean and std.dev: (109.62, 103.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 801) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.32, 75.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (42, 67, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 882) [M::mem_pestat] mean and std.dev: (94.03, 101.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1162) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (586, 1079, 1486) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3286) [M::mem_pestat] mean and std.dev: (1128.83, 689.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4186) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 911.430 CPU sec, 53.640 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (81, 667148, 37, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (40, 71, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (96.99, 82.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 620) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.34, 75.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 62, 2614) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7792) [M::mem_pestat] mean and std.dev: (1093.68, 1775.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10381) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (813, 832, 1382) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2520) [M::mem_pestat] mean and std.dev: (940.30, 547.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3129) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 906.028 CPU sec, 40.333 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (82, 666514, 36, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (51, 79, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (117.54, 105.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 671) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.31, 75.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 110, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627) [M::mem_pestat] mean and std.dev: (90.79, 85.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 825) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (547, 1020, 1843) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4435) [M::mem_pestat] mean and std.dev: (982.17, 618.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5731) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 898.463 CPU sec, 51.388 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (74, 667290, 26, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (39, 62, 136) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 330) [M::mem_pestat] mean and std.dev: (80.33, 68.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 427) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.42, 75.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 42, 923) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2735) [M::mem_pestat] mean and std.dev: (363.78, 710.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3641) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (538, 1086, 1328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2908) [M::mem_pestat] mean and std.dev: (1025.07, 656.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3698) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 905.355 CPU sec, 40.318 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (84, 667040, 26, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (43, 71, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (91.40, 68.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 547) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.40, 75.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (42, 138, 1297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3807) [M::mem_pestat] mean and std.dev: (471.61, 710.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5062) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (823, 1512, 2643) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6283) [M::mem_pestat] mean and std.dev: (1667.50, 1140.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8103) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 898.007 CPU sec, 51.539 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (79, 667372, 29, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (42, 71, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 438) [M::mem_pestat] mean and std.dev: (98.81, 77.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 570) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.45, 75.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (46, 101, 368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1012) [M::mem_pestat] mean and std.dev: (154.65, 190.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1334) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (209, 557, 1127) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2963) [M::mem_pestat] mean and std.dev: (630.00, 465.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3881) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 899.130 CPU sec, 39.980 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (92, 666932, 29, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (44, 61, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 431) [M::mem_pestat] mean and std.dev: (85.15, 66.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 560) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.49, 75.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 93, 378) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1082) [M::mem_pestat] mean and std.dev: (146.20, 215.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1434) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 906.367 CPU sec, 51.941 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (81, 666815, 21, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (41, 95, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (105.15, 82.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 661) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.38, 75.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (56, 81, 108) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 212) [M::mem_pestat] mean and std.dev: (67.82, 28.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 264) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (523, 842, 2194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5536) [M::mem_pestat] mean and std.dev: (1321.20, 1156.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7207) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 906.864 CPU sec, 40.231 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (70, 667138, 20, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (35, 74, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 443) [M::mem_pestat] mean and std.dev: (99.60, 79.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 579) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.47, 75.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (72, 124, 2693) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7935) [M::mem_pestat] mean and std.dev: (1010.58, 1541.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10556) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (487, 688, 2349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6073) [M::mem_pestat] mean and std.dev: (1194.67, 1012.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7935) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 897.789 CPU sec, 51.252 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (75, 666434, 40, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (35, 57, 130) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 320) [M::mem_pestat] mean and std.dev: (77.43, 60.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 415) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.44, 75.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (53, 111, 1800) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5294) [M::mem_pestat] mean and std.dev: (938.33, 1447.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7041) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 903.371 CPU sec, 40.039 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (76, 666920, 40, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (50, 75, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 380) [M::mem_pestat] mean and std.dev: (92.17, 68.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 490) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.38, 75.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (41, 91, 351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 971) [M::mem_pestat] mean and std.dev: (80.03, 65.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1281) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (966, 1112, 1672) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3084) [M::mem_pestat] mean and std.dev: (1211.60, 330.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3790) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 901.367 CPU sec, 52.185 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (89, 666687, 22, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (46, 63, 159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 385) [M::mem_pestat] mean and std.dev: (86.75, 66.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 498) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.33, 75.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 143, 2755) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8199) [M::mem_pestat] mean and std.dev: (1270.59, 1929.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10921) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (584, 1042, 2669) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6839) [M::mem_pestat] mean and std.dev: (1722.85, 1651.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8924) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 900.647 CPU sec, 40.075 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (76, 667322, 27, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (39, 56, 129) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 309) [M::mem_pestat] mean and std.dev: (77.49, 63.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 399) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.33, 75.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (52, 98, 2061) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6079) [M::mem_pestat] mean and std.dev: (882.88, 1562.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8088) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 901.170 CPU sec, 50.930 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (81, 667026, 26, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (43, 94, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 535) [M::mem_pestat] mean and std.dev: (95.33, 83.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 699) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.33, 75.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (56, 117, 1093) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3167) [M::mem_pestat] mean and std.dev: (550.71, 904.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4204) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (684, 1274, 3325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8607) [M::mem_pestat] mean and std.dev: (2023.15, 2136.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11248) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 900.808 CPU sec, 40.121 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (74, 667295, 25, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (46, 94, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (99.81, 78.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 642) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.42, 75.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 52, 1044) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3080) [M::mem_pestat] mean and std.dev: (341.32, 790.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4098) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (946, 1683, 1809) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3535) [M::mem_pestat] mean and std.dev: (1348.27, 872.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4838) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 898.346 CPU sec, 50.241 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (83, 667004, 20, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (44, 70, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 467) [M::mem_pestat] mean and std.dev: (84.71, 67.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 608) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (11, 521) [M::mem_pestat] mean and std.dev: (269.22, 75.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 58, 121) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 311) [M::mem_pestat] mean and std.dev: (59.11, 45.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 406) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (722, 1069, 1337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2567) [M::mem_pestat] mean and std.dev: (1035.25, 581.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3361) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 899.323 CPU sec, 50.942 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (79, 666908, 35, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (42, 68, 134) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 318) [M::mem_pestat] mean and std.dev: (84.86, 61.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 410) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.34, 75.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 69, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 677) [M::mem_pestat] mean and std.dev: (82.83, 91.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 891) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (361, 678, 2516) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6826) [M::mem_pestat] mean and std.dev: (1353.58, 1641.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8981) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 904.543 CPU sec, 40.305 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (68, 666885, 31, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (35, 69, 150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 380) [M::mem_pestat] mean and std.dev: (85.25, 73.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 495) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.46, 75.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (45, 137, 1271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3723) [M::mem_pestat] mean and std.dev: (523.07, 857.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4949) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (598, 1579, 2140) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5224) [M::mem_pestat] mean and std.dev: (1218.25, 871.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6766) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 899.611 CPU sec, 51.377 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (66, 667204, 31, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (40, 67, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424) [M::mem_pestat] mean and std.dev: (94.56, 72.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 552) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.41, 75.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 47, 101) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 243) [M::mem_pestat] mean and std.dev: (50.46, 34.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 314) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (773, 924, 975) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (369, 1379) [M::mem_pestat] mean and std.dev: (839.71, 162.57) [M::mem_pestat] low and high boundaries for proper pairs: (167, 1581) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 899.476 CPU sec, 39.937 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (68, 667245, 13, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (39, 56, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 429) [M::mem_pestat] mean and std.dev: (96.02, 84.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 559) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.41, 75.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (51, 108, 3037) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9009) [M::mem_pestat] mean and std.dev: (1097.77, 1682.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11995) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (736, 1512, 2170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5038) [M::mem_pestat] mean and std.dev: (1471.45, 1197.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6472) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 898.929 CPU sec, 50.880 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (79, 666240, 18, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (47, 73, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 398) [M::mem_pestat] mean and std.dev: (88.17, 65.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 515) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.33, 75.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (53, 124, 1542) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4520) [M::mem_pestat] mean and std.dev: (630.31, 1172.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6009) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (379, 732, 2269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6049) [M::mem_pestat] mean and std.dev: (987.54, 837.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7939) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 899.666 CPU sec, 39.854 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (63, 666997, 31, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (46, 72, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 439) [M::mem_pestat] mean and std.dev: (93.71, 73.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 570) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.36, 75.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (42, 79, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 582) [M::mem_pestat] mean and std.dev: (94.68, 110.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 762) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (321, 594, 1588) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4122) [M::mem_pestat] mean and std.dev: (1030.00, 908.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5389) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 904.152 CPU sec, 51.527 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (96, 666973, 29, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (43, 103, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 553) [M::mem_pestat] mean and std.dev: (106.00, 99.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 723) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.29, 75.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 110, 3949) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11777) [M::mem_pestat] mean and std.dev: (1492.17, 2375.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15691) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (567, 863, 2654) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6828) [M::mem_pestat] mean and std.dev: (1132.17, 1030.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8915) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 905.528 CPU sec, 40.329 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (58, 667372, 31, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (41, 84, 143) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 347) [M::mem_pestat] mean and std.dev: (85.52, 67.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 449) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.40, 75.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 88, 403) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1145) [M::mem_pestat] mean and std.dev: (99.20, 126.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1516) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (371, 933, 1386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3416) [M::mem_pestat] mean and std.dev: (889.10, 601.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4431) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 898.720 CPU sec, 51.990 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (58, 666943, 32, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (43, 74, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 511) [M::mem_pestat] mean and std.dev: (102.60, 108.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 667) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.49, 75.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (42, 99, 1350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3966) [M::mem_pestat] mean and std.dev: (561.68, 940.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5274) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (803, 1180, 1655) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3359) [M::mem_pestat] mean and std.dev: (1294.89, 734.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4232) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 901.793 CPU sec, 40.093 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (76, 666802, 29, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (44, 88, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 398) [M::mem_pestat] mean and std.dev: (87.62, 64.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 516) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.32, 75.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 71, 748) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2182) [M::mem_pestat] mean and std.dev: (129.12, 206.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2899) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 899.966 CPU sec, 52.399 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (69, 666363, 22, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (41, 91, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488) [M::mem_pestat] mean and std.dev: (98.58, 70.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 637) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.37, 75.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 61, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913) [M::mem_pestat] mean and std.dev: (111.67, 193.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1212) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (320, 1020, 1384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3512) [M::mem_pestat] mean and std.dev: (868.00, 515.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4576) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 903.098 CPU sec, 40.260 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (74, 667035, 33, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (41, 74, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 611) [M::mem_pestat] mean and std.dev: (92.05, 84.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 801) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.25, 75.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 36, 84) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 224) [M::mem_pestat] mean and std.dev: (43.13, 43.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 294) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (558, 904, 1513) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3423) [M::mem_pestat] mean and std.dev: (890.83, 535.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4378) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 902.826 CPU sec, 51.694 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (86, 667093, 30, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (40, 68, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (88.41, 72.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 620) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.48, 75.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 127, 1439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4273) [M::mem_pestat] mean and std.dev: (323.72, 547.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5690) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (632, 819, 1190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2306) [M::mem_pestat] mean and std.dev: (952.71, 521.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3038) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 899.553 CPU sec, 39.837 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (61, 666783, 22, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (37, 59, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (82.77, 67.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 625) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.44, 75.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 93, 1792) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5320) [M::mem_pestat] mean and std.dev: (910.91, 1517.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7084) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (274, 1126, 2011) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5485) [M::mem_pestat] mean and std.dev: (1136.56, 1168.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7222) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 907.675 CPU sec, 47.953 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (64, 666746, 30, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (37, 77, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 604) [M::mem_pestat] mean and std.dev: (107.73, 116.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 793) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.39, 75.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 54, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 446) [M::mem_pestat] mean and std.dev: (82.12, 84.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (463, 758, 1346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3112) [M::mem_pestat] mean and std.dev: (734.17, 466.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3995) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 910.357 CPU sec, 53.149 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (84, 666622, 37, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (34, 53, 145) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 367) [M::mem_pestat] mean and std.dev: (83.83, 75.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 478) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.41, 75.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (40, 133, 2500) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7420) [M::mem_pestat] mean and std.dev: (1276.89, 1763.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9880) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1038, 1505, 2400) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5124) [M::mem_pestat] mean and std.dev: (1751.18, 1211.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6595) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 909.186 CPU sec, 40.604 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (90, 667156, 27, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (40, 70, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (97.01, 87.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 616) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.56, 75.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (72, 122, 2251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6609) [M::mem_pestat] mean and std.dev: (1057.89, 1546.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8788) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (631, 1301, 2534) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6340) [M::mem_pestat] mean and std.dev: (1498.38, 1053.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8243) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 900.635 CPU sec, 52.905 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (80, 666857, 25, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (38, 59, 140) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 344) [M::mem_pestat] mean and std.dev: (76.87, 62.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 446) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.34, 75.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (40, 89, 1811) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5353) [M::mem_pestat] mean and std.dev: (726.79, 1336.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7124) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 905.063 CPU sec, 40.446 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (69, 667085, 32, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (43, 78, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745) [M::mem_pestat] mean and std.dev: (120.05, 128.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 979) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.33, 75.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (74, 174, 2769) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8159) [M::mem_pestat] mean and std.dev: (1354.69, 1806.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10854) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (452, 1022, 1287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2957) [M::mem_pestat] mean and std.dev: (855.91, 519.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3792) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 898.162 CPU sec, 52.086 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (77, 666650, 27, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (37, 56, 145) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 361) [M::mem_pestat] mean and std.dev: (77.41, 56.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 469) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.42, 75.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (38, 112, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (75.71, 53.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (239, 772, 1297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3413) [M::mem_pestat] mean and std.dev: (914.65, 704.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4471) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 906.964 CPU sec, 40.236 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (91, 667091, 33, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (44, 82, 151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 365) [M::mem_pestat] mean and std.dev: (94.27, 68.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 472) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.31, 75.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 78, 693) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2009) [M::mem_pestat] mean and std.dev: (161.37, 340.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2667) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (853, 2491, 3787) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9655) [M::mem_pestat] mean and std.dev: (2790.45, 2319.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12589) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 898.048 CPU sec, 51.952 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 666779, 36, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 105, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 437) [M::mem_pestat] mean and std.dev: (105.81, 62.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 564) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.48, 75.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 108, 2672) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7952) [M::mem_pestat] mean and std.dev: (1105.25, 1536.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10592) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 900.985 CPU sec, 40.210 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (79, 666984, 38, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (41, 65, 154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 380) [M::mem_pestat] mean and std.dev: (83.91, 70.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 493) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.43, 75.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 117, 423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1203) [M::mem_pestat] mean and std.dev: (111.37, 109.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1593) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (669, 852, 1904) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4374) [M::mem_pestat] mean and std.dev: (1103.79, 808.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5609) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 904.238 CPU sec, 51.403 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (68, 666769, 22, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (41, 74, 149) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 365) [M::mem_pestat] mean and std.dev: (84.49, 60.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 473) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.32, 75.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 63, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1290) [M::mem_pestat] mean and std.dev: (82.94, 114.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1710) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (187, 728, 1979) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5563) [M::mem_pestat] mean and std.dev: (834.58, 809.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7355) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 902.865 CPU sec, 40.024 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (79, 666980, 28, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (44, 67, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 452) [M::mem_pestat] mean and std.dev: (93.96, 87.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 588) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.34, 75.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 53, 157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (52.55, 44.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 553) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (637, 909, 1334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2728) [M::mem_pestat] mean and std.dev: (962.33, 596.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3425) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 905.427 CPU sec, 53.236 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (71, 666880, 30, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (42, 69, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402) [M::mem_pestat] mean and std.dev: (89.86, 73.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 522) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.52, 75.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 72, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 831) [M::mem_pestat] mean and std.dev: (75.50, 86.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1098) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1019, 1405, 1903) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3671) [M::mem_pestat] mean and std.dev: (1268.93, 675.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4555) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 907.396 CPU sec, 40.237 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (78, 666885, 29, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (42, 83, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462) [M::mem_pestat] mean and std.dev: (91.65, 77.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.43, 75.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (44, 102, 701) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2015) [M::mem_pestat] mean and std.dev: (321.93, 515.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2672) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (777, 888, 1668) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3450) [M::mem_pestat] mean and std.dev: (766.75, 438.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4341) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 905.370 CPU sec, 52.564 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (59, 666892, 35, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (42, 70, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 456) [M::mem_pestat] mean and std.dev: (86.67, 67.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.25, 75.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (46, 88, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400) [M::mem_pestat] mean and std.dev: (88.59, 79.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 518) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (577, 864, 1819) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4303) [M::mem_pestat] mean and std.dev: (1003.71, 660.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5545) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 906.171 CPU sec, 40.108 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (77, 666893, 27, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (39, 57, 113) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 261) [M::mem_pestat] mean and std.dev: (72.73, 52.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 335) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.44, 75.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 83, 149) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 389) [M::mem_pestat] mean and std.dev: (67.27, 50.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 509) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (623, 833, 2477) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6185) [M::mem_pestat] mean and std.dev: (1220.40, 955.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8039) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 900.197 CPU sec, 44.595 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (70, 666743, 23, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (32, 66, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 557) [M::mem_pestat] mean and std.dev: (100.68, 94.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 732) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.32, 75.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (38, 65, 417) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1175) [M::mem_pestat] mean and std.dev: (95.39, 107.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1554) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (178, 848, 1145) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3079) [M::mem_pestat] mean and std.dev: (685.10, 590.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4046) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 911.032 CPU sec, 51.814 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (66, 667748, 22, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (39, 64, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402) [M::mem_pestat] mean and std.dev: (92.10, 85.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 523) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.28, 75.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 54, 139) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 385) [M::mem_pestat] mean and std.dev: (61.00, 48.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 508) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (710, 1035, 1898) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4274) [M::mem_pestat] mean and std.dev: (1162.24, 631.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5462) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 902.507 CPU sec, 40.149 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (61, 666847, 43, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (35, 59, 150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 380) [M::mem_pestat] mean and std.dev: (79.21, 74.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 495) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.36, 75.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 59, 1426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4232) [M::mem_pestat] mean and std.dev: (423.26, 908.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5635) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (853, 934, 2168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4798) [M::mem_pestat] mean and std.dev: (1238.55, 1067.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6113) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 901.482 CPU sec, 52.944 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (76, 666600, 33, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (46, 90, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (106.25, 88.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.34, 75.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (45, 129, 744) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2142) [M::mem_pestat] mean and std.dev: (193.59, 286.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2841) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (605, 842, 1299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2687) [M::mem_pestat] mean and std.dev: (898.36, 554.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3381) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 899.504 CPU sec, 39.905 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (73, 666478, 32, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (53, 91, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 467) [M::mem_pestat] mean and std.dev: (97.83, 64.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 605) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.41, 75.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (53, 115, 1913) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5633) [M::mem_pestat] mean and std.dev: (793.32, 1297.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7493) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (526, 689, 1557) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3619) [M::mem_pestat] mean and std.dev: (865.55, 645.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4650) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 902.475 CPU sec, 52.173 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (87, 666345, 35, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (37, 76, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 445) [M::mem_pestat] mean and std.dev: (86.95, 80.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 581) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.59, 75.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 60, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 752) [M::mem_pestat] mean and std.dev: (114.52, 155.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 993) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (477, 663, 1176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2574) [M::mem_pestat] mean and std.dev: (763.17, 552.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3273) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 907.837 CPU sec, 40.298 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (63, 666612, 32, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (44, 65, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476) [M::mem_pestat] mean and std.dev: (92.91, 77.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 620) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.35, 75.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (39, 86, 648) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1866) [M::mem_pestat] mean and std.dev: (183.73, 354.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2475) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (835, 948, 2203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4939) [M::mem_pestat] mean and std.dev: (1402.57, 1023.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6307) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 905.319 CPU sec, 51.842 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (63, 666829, 24, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (36, 85, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 456) [M::mem_pestat] mean and std.dev: (103.60, 92.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 596) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.29, 75.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (47, 97, 1521) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4469) [M::mem_pestat] mean and std.dev: (577.55, 950.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5943) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (397, 1459, 3012) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8242) [M::mem_pestat] mean and std.dev: (1639.73, 1363.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10857) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 902.333 CPU sec, 39.945 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (77, 666842, 35, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (37, 60, 130) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 316) [M::mem_pestat] mean and std.dev: (75.99, 59.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 409) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.36, 75.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (64, 134, 2874) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8494) [M::mem_pestat] mean and std.dev: (1224.12, 1847.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11304) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (751, 1061, 2585) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6253) [M::mem_pestat] mean and std.dev: (1562.62, 1277.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8087) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 905.526 CPU sec, 51.875 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (89, 666346, 29, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (47, 78, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 389) [M::mem_pestat] mean and std.dev: (97.65, 73.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 503) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.38, 75.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 58, 677) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1973) [M::mem_pestat] mean and std.dev: (191.16, 404.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2621) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (785, 957, 1254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2192) [M::mem_pestat] mean and std.dev: (932.42, 272.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2661) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 907.276 CPU sec, 40.147 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (72, 667115, 30, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (52, 111, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 586) [M::mem_pestat] mean and std.dev: (123.24, 97.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 764) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.31, 75.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 66, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (59.12, 54.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 628) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 905.082 CPU sec, 52.643 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (86, 667101, 22, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (41, 85, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (102.11, 88.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.32, 75.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (56, 162, 1704) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5000) [M::mem_pestat] mean and std.dev: (975.00, 1433.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6707) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (645, 729, 1292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2586) [M::mem_pestat] mean and std.dev: (859.07, 492.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3233) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 900.691 CPU sec, 39.990 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (61, 666686, 30, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (51, 74, 154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 360) [M::mem_pestat] mean and std.dev: (92.51, 64.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 463) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.37, 75.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (63, 176, 2712) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8010) [M::mem_pestat] mean and std.dev: (949.96, 1443.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10659) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (738, 890, 1122) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1890) [M::mem_pestat] mean and std.dev: (873.73, 199.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2274) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 903.533 CPU sec, 52.059 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (69, 667238, 29, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (45, 75, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (95.24, 80.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 621) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.39, 75.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 119, 1914) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5672) [M::mem_pestat] mean and std.dev: (662.22, 1073.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7551) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (817, 1178, 1939) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4183) [M::mem_pestat] mean and std.dev: (1256.78, 829.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5305) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 902.998 CPU sec, 40.242 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (89, 667088, 23, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (40, 61, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (94.55, 89.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 648) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.36, 75.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 99, 3177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9471) [M::mem_pestat] mean and std.dev: (1773.57, 2713.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12627) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (215, 758, 1177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3101) [M::mem_pestat] mean and std.dev: (775.67, 657.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4063) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 901.800 CPU sec, 42.723 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (72, 666576, 27, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (35, 85, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476) [M::mem_pestat] mean and std.dev: (95.82, 93.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.25, 75.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (47, 98, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656) [M::mem_pestat] mean and std.dev: (95.68, 82.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 859) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (218, 988, 2364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6656) [M::mem_pestat] mean and std.dev: (1061.23, 937.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8802) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 914.064 CPU sec, 52.601 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (79, 666581, 32, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (44, 69, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (97.79, 75.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 640) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.39, 75.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (47, 103, 695) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1991) [M::mem_pestat] mean and std.dev: (147.00, 252.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2639) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (795, 970, 1750) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3660) [M::mem_pestat] mean and std.dev: (1036.07, 531.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4615) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 909.418 CPU sec, 40.372 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (72, 666861, 29, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (49, 78, 141) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 325) [M::mem_pestat] mean and std.dev: (90.41, 61.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 417) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.38, 75.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 86, 554) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1596) [M::mem_pestat] mean and std.dev: (190.32, 284.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2117) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 905.377 CPU sec, 52.357 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (83, 666934, 28, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (37, 63, 159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403) [M::mem_pestat] mean and std.dev: (80.67, 60.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 525) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.49, 75.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 49, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 457) [M::mem_pestat] mean and std.dev: (64.17, 69.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (663, 859, 1357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2745) [M::mem_pestat] mean and std.dev: (867.40, 472.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3439) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 901.831 CPU sec, 40.076 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (82, 666270, 30, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (40, 67, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454) [M::mem_pestat] mean and std.dev: (101.65, 86.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 592) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.45, 75.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (40, 72, 143) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 349) [M::mem_pestat] mean and std.dev: (60.78, 40.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 452) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (733, 793, 2219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5191) [M::mem_pestat] mean and std.dev: (1106.10, 753.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6677) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 906.070 CPU sec, 51.150 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (81, 666828, 31, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (41, 61, 135) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 323) [M::mem_pestat] mean and std.dev: (72.64, 53.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 417) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.47, 75.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 129, 2456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7296) [M::mem_pestat] mean and std.dev: (1122.90, 1713.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9716) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 905.395 CPU sec, 40.095 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (59, 667446, 31, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (39, 57, 147) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 363) [M::mem_pestat] mean and std.dev: (87.11, 74.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 471) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.49, 75.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (41, 98, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (83.08, 55.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 601) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (453, 1036, 3875) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10719) [M::mem_pestat] mean and std.dev: (2003.85, 2537.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14141) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 905.212 CPU sec, 53.466 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (75, 666224, 26, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (41, 67, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (90.29, 67.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 665) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.22, 75.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 96, 2851) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8481) [M::mem_pestat] mean and std.dev: (1013.00, 1565.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11296) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (653, 1133, 1570) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3404) [M::mem_pestat] mean and std.dev: (1065.71, 713.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4321) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 905.256 CPU sec, 40.156 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (86, 666908, 35, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (41, 76, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (98.36, 74.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 653) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.40, 75.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 63, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918) [M::mem_pestat] mean and std.dev: (78.79, 86.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1212) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (158, 1104, 2014) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5726) [M::mem_pestat] mean and std.dev: (1090.07, 848.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7582) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 903.674 CPU sec, 52.390 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (76, 667061, 29, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (38, 81, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 527) [M::mem_pestat] mean and std.dev: (105.57, 97.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 690) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.39, 75.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (68, 146, 1226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3542) [M::mem_pestat] mean and std.dev: (666.28, 947.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4700) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (777, 1314, 1974) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4368) [M::mem_pestat] mean and std.dev: (1208.62, 685.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5565) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 901.963 CPU sec, 40.005 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (73, 666897, 26, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (46, 69, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454) [M::mem_pestat] mean and std.dev: (97.38, 81.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.41, 75.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (39, 114, 926) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2700) [M::mem_pestat] mean and std.dev: (266.95, 451.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3587) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (440, 1106, 2006) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5138) [M::mem_pestat] mean and std.dev: (1157.21, 797.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6704) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 902.701 CPU sec, 52.342 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (82, 666738, 35, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (38, 57, 127) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 305) [M::mem_pestat] mean and std.dev: (72.11, 54.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 394) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.32, 75.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 54, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1020) [M::mem_pestat] mean and std.dev: (72.70, 74.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1349) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (233, 1159, 2151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5987) [M::mem_pestat] mean and std.dev: (1273.85, 1298.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7905) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 901.370 CPU sec, 39.810 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (63, 667292, 24, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (39, 81, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (96.72, 69.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.36, 75.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (50, 121, 1165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3395) [M::mem_pestat] mean and std.dev: (354.35, 728.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4510) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (570, 884, 1143) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2289) [M::mem_pestat] mean and std.dev: (779.16, 508.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2862) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 906.122 CPU sec, 53.838 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (75, 666465, 35, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (35, 55, 128) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 314) [M::mem_pestat] mean and std.dev: (69.25, 50.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 407) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.47, 75.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (57, 100, 424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1158) [M::mem_pestat] mean and std.dev: (129.04, 191.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1525) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (642, 1064, 1661) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3699) [M::mem_pestat] mean and std.dev: (923.82, 481.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4718) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 905.267 CPU sec, 40.167 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (63, 667518, 36, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (39, 74, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (92.59, 76.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (216, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (12, 522) [M::mem_pestat] mean and std.dev: (269.48, 75.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 624) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 100, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 832) [M::mem_pestat] mean and std.dev: (79.10, 80.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1102) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (638, 1116, 1580) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3464) [M::mem_pestat] mean and std.dev: (1072.50, 613.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4406) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 905.969 CPU sec, 51.680 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (75, 667029, 35, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (42, 85, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 489) [M::mem_pestat] mean and std.dev: (110.69, 102.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 638) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.40, 75.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 116, 2275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6759) [M::mem_pestat] mean and std.dev: (1125.71, 1660.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9001) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (495, 667, 1248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2754) [M::mem_pestat] mean and std.dev: (805.54, 573.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3507) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 902.357 CPU sec, 39.888 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (61, 666833, 26, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (41, 73, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 605) [M::mem_pestat] mean and std.dev: (114.53, 128.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 793) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.47, 75.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 106, 1631) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4839) [M::mem_pestat] mean and std.dev: (779.21, 1363.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6443) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (623, 1117, 1839) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4271) [M::mem_pestat] mean and std.dev: (1224.24, 771.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5487) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 908.499 CPU sec, 52.016 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (65, 667195, 34, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (46, 101, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (96.58, 71.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 778) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.42, 75.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (43, 58, 533) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1513) [M::mem_pestat] mean and std.dev: (125.07, 229.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2003) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (663, 1024, 1469) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3081) [M::mem_pestat] mean and std.dev: (981.24, 526.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3887) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 907.829 CPU sec, 40.427 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (91, 667207, 24, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (41, 72, 159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 395) [M::mem_pestat] mean and std.dev: (96.82, 81.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 513) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.44, 75.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (46, 86, 538) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1522) [M::mem_pestat] mean and std.dev: (127.50, 197.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2014) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (622, 1220, 1654) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3718) [M::mem_pestat] mean and std.dev: (1204.44, 907.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4834) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 906.078 CPU sec, 53.519 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (76, 666713, 32, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (36, 55, 156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 396) [M::mem_pestat] mean and std.dev: (89.27, 83.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 516) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.41, 75.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 46, 138) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 366) [M::mem_pestat] mean and std.dev: (66.76, 53.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 480) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 903.689 CPU sec, 40.172 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (62, 667031, 30, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (34, 68, 126) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 310) [M::mem_pestat] mean and std.dev: (82.37, 57.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 402) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.64, 75.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (40, 94, 146) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 358) [M::mem_pestat] mean and std.dev: (73.12, 53.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 464) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (688, 853, 1838) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4138) [M::mem_pestat] mean and std.dev: (968.75, 664.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5288) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 907.677 CPU sec, 53.042 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (81, 667262, 23, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (43, 63, 141) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 337) [M::mem_pestat] mean and std.dev: (73.54, 50.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 435) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.44, 75.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (52, 64, 149) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 343) [M::mem_pestat] mean and std.dev: (83.90, 50.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 440) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (641, 709, 1316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2666) [M::mem_pestat] mean and std.dev: (913.91, 603.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3341) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 899.403 CPU sec, 39.918 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (68, 666174, 21, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (38, 66, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497) [M::mem_pestat] mean and std.dev: (87.34, 85.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 650) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.36, 75.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (48, 105, 3389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10071) [M::mem_pestat] mean and std.dev: (1458.24, 2526.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13412) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (480, 829, 1843) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4569) [M::mem_pestat] mean and std.dev: (1074.30, 1063.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5932) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 909.829 CPU sec, 51.951 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (70, 667161, 30, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (40, 75, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 541) [M::mem_pestat] mean and std.dev: (106.05, 99.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 708) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.24, 75.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (44, 58, 137) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 323) [M::mem_pestat] mean and std.dev: (88.35, 83.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 423) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (689, 873, 2137) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5033) [M::mem_pestat] mean and std.dev: (1166.27, 841.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6481) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 910.664 CPU sec, 40.461 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (81, 667181, 24, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (35, 54, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 422) [M::mem_pestat] mean and std.dev: (75.87, 64.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 551) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (216, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (12, 522) [M::mem_pestat] mean and std.dev: (269.50, 75.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 624) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (43, 75, 982) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2860) [M::mem_pestat] mean and std.dev: (231.38, 418.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3799) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (311, 873, 2349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6425) [M::mem_pestat] mean and std.dev: (1231.57, 1210.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8463) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 908.954 CPU sec, 53.494 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (89, 666906, 37, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (38, 90, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (97.36, 85.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.28, 75.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (43, 68, 531) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1507) [M::mem_pestat] mean and std.dev: (123.43, 248.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1995) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (551, 772, 1275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2723) [M::mem_pestat] mean and std.dev: (806.88, 482.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3447) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 901.492 CPU sec, 39.944 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (66, 667177, 28, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (33, 49, 113) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 273) [M::mem_pestat] mean and std.dev: (64.14, 48.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 353) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.40, 75.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (59, 141, 2682) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7928) [M::mem_pestat] mean and std.dev: (1017.59, 1561.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10551) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (312, 643, 1269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3183) [M::mem_pestat] mean and std.dev: (781.07, 659.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4140) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 902.510 CPU sec, 52.760 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (64, 667136, 31, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (36, 60, 135) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 333) [M::mem_pestat] mean and std.dev: (79.52, 64.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 432) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.29, 75.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 50, 141) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 365) [M::mem_pestat] mean and std.dev: (57.52, 51.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 477) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (333, 749, 1232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3030) [M::mem_pestat] mean and std.dev: (764.57, 477.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3929) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 906.564 CPU sec, 40.350 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (80, 666774, 19, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (51, 80, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 381) [M::mem_pestat] mean and std.dev: (86.01, 55.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 491) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.52, 75.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 54, 119) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 301) [M::mem_pestat] mean and std.dev: (59.44, 45.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 392) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 909.568 CPU sec, 53.170 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (75, 667453, 25, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (38, 63, 144) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 356) [M::mem_pestat] mean and std.dev: (77.23, 57.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.30, 75.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 96, 2089) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6217) [M::mem_pestat] mean and std.dev: (885.92, 1486.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8281) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (700, 805, 1058) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1774) [M::mem_pestat] mean and std.dev: (775.33, 367.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2244) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 914.010 CPU sec, 53.520 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (93, 667064, 26, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (42, 71, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 504) [M::mem_pestat] mean and std.dev: (100.40, 99.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 658) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.30, 75.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 115, 1083) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3183) [M::mem_pestat] mean and std.dev: (380.91, 835.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4233) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (472, 821, 1481) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3499) [M::mem_pestat] mean and std.dev: (943.75, 700.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4508) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 911.086 CPU sec, 40.568 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (68, 666981, 23, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (44, 111, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 575) [M::mem_pestat] mean and std.dev: (114.00, 100.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 752) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.49, 75.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 130, 1772) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5248) [M::mem_pestat] mean and std.dev: (773.71, 1371.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6986) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (455, 787, 1366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3188) [M::mem_pestat] mean and std.dev: (910.85, 539.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4099) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 899.539 CPU sec, 52.538 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (69, 666149, 22, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (47, 88, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (119.43, 102.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 655) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (11, 521) [M::mem_pestat] mean and std.dev: (269.10, 75.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 83, 1729) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5137) [M::mem_pestat] mean and std.dev: (878.86, 1369.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6841) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 900.984 CPU sec, 40.035 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (73, 666774, 27, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (39, 58, 158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 396) [M::mem_pestat] mean and std.dev: (80.74, 63.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 515) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.26, 75.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (55, 124, 924) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2662) [M::mem_pestat] mean and std.dev: (173.48, 249.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3531) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (239, 675, 1220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3182) [M::mem_pestat] mean and std.dev: (832.80, 887.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4384) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 905.999 CPU sec, 52.263 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (63, 667091, 23, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (40, 69, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622) [M::mem_pestat] mean and std.dev: (116.04, 125.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 816) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.35, 75.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (98, 125, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632) [M::mem_pestat] mean and std.dev: (109.94, 57.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 810) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (643, 939, 1192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2290) [M::mem_pestat] mean and std.dev: (817.00, 330.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2839) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 902.915 CPU sec, 40.200 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (69, 666683, 31, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (41, 60, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668) [M::mem_pestat] mean and std.dev: (123.42, 143.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 877) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (11, 521) [M::mem_pestat] mean and std.dev: (269.16, 75.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 55, 142) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 360) [M::mem_pestat] mean and std.dev: (66.19, 69.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 469) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (529, 968, 1561) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3625) [M::mem_pestat] mean and std.dev: (1059.77, 772.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4657) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 908.482 CPU sec, 53.014 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (80, 667056, 30, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (44, 89, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 524) [M::mem_pestat] mean and std.dev: (98.45, 78.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 684) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (216, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (12, 522) [M::mem_pestat] mean and std.dev: (269.49, 75.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 624) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (44, 78, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (89.20, 87.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 540) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (545, 1320, 3258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8684) [M::mem_pestat] mean and std.dev: (1826.75, 2045.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11397) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 904.469 CPU sec, 40.204 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (71, 666561, 28, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (45, 65, 159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 387) [M::mem_pestat] mean and std.dev: (86.60, 71.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 501) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.44, 75.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (46, 196, 4601) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13711) [M::mem_pestat] mean and std.dev: (2199.04, 2996.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18266) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (635, 1081, 1443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3059) [M::mem_pestat] mean and std.dev: (984.94, 540.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3867) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 914.036 CPU sec, 54.152 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (83, 666620, 25, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (43, 74, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 598) [M::mem_pestat] mean and std.dev: (103.89, 97.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 783) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.29, 75.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 54, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 442) [M::mem_pestat] mean and std.dev: (66.29, 71.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 583) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (929, 1133, 1918) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3896) [M::mem_pestat] mean and std.dev: (1252.36, 611.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4885) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 909.261 CPU sec, 40.537 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (90, 666096, 27, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (40, 75, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (88.77, 70.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 632) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.36, 75.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 68, 2785) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8287) [M::mem_pestat] mean and std.dev: (1164.56, 2218.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11038) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (396, 632, 1491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3681) [M::mem_pestat] mean and std.dev: (977.73, 910.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4776) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 915.437 CPU sec, 53.995 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (70, 666837, 38, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (43, 60, 151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 367) [M::mem_pestat] mean and std.dev: (79.97, 57.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 475) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.52, 75.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 48, 126) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 324) [M::mem_pestat] mean and std.dev: (51.06, 38.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 423) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (532, 902, 1048) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2080) [M::mem_pestat] mean and std.dev: (720.62, 366.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2596) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 906.271 CPU sec, 40.428 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (87, 667019, 31, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (44, 89, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 440) [M::mem_pestat] mean and std.dev: (105.69, 79.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 572) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.39, 75.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 74, 423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1225) [M::mem_pestat] mean and std.dev: (89.92, 124.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1626) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 906.277 CPU sec, 52.650 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (57, 666769, 33, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (44, 95, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 740) [M::mem_pestat] mean and std.dev: (148.02, 160.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 972) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.36, 75.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (44, 71, 129) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 299) [M::mem_pestat] mean and std.dev: (69.82, 46.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 384) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (632, 819, 1583) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3485) [M::mem_pestat] mean and std.dev: (1067.09, 881.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4592) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 908.842 CPU sec, 40.481 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (82, 666786, 26, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (35, 51, 111) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 263) [M::mem_pestat] mean and std.dev: (72.21, 57.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 339) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.45, 75.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (62, 127, 812) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2312) [M::mem_pestat] mean and std.dev: (257.65, 332.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3062) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (714, 1434, 1750) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3822) [M::mem_pestat] mean and std.dev: (1369.17, 819.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4858) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 913.166 CPU sec, 53.927 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (72, 666959, 25, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (43, 73, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 436) [M::mem_pestat] mean and std.dev: (91.89, 69.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 567) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.52, 75.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 67, 533) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1539) [M::mem_pestat] mean and std.dev: (85.45, 113.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2042) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (714, 1119, 2394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5754) [M::mem_pestat] mean and std.dev: (1342.00, 951.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7434) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 912.223 CPU sec, 55.025 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (72, 666896, 29, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (44, 70, 158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 386) [M::mem_pestat] mean and std.dev: (84.16, 58.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 500) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.26, 75.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 97, 1618) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4786) [M::mem_pestat] mean and std.dev: (815.04, 1334.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6370) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (470, 883, 1538) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3674) [M::mem_pestat] mean and std.dev: (962.05, 626.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4742) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 905.423 CPU sec, 40.600 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (85, 666354, 25, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (41, 60, 147) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 359) [M::mem_pestat] mean and std.dev: (80.62, 60.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 465) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.32, 75.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 139, 2141) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6363) [M::mem_pestat] mean and std.dev: (906.68, 1278.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8474) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 920.014 CPU sec, 54.520 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (64, 666474, 32, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (34, 54, 137) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 343) [M::mem_pestat] mean and std.dev: (78.43, 68.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 446) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.34, 75.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (40, 97, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649) [M::mem_pestat] mean and std.dev: (77.08, 59.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 852) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (968, 2054, 4329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11051) [M::mem_pestat] mean and std.dev: (2453.00, 2185.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14412) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 909.477 CPU sec, 40.420 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (75, 666834, 30, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (39, 54, 114) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 264) [M::mem_pestat] mean and std.dev: (67.22, 44.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 339) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.29, 75.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 55, 133) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 365) [M::mem_pestat] mean and std.dev: (58.54, 51.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 481) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (552, 865, 1799) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4293) [M::mem_pestat] mean and std.dev: (1144.00, 1017.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5540) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 904.593 CPU sec, 50.806 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (65, 666960, 33, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (42, 62, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (90.72, 82.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 674) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.55, 75.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 85, 1749) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5197) [M::mem_pestat] mean and std.dev: (660.97, 1268.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6921) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (886, 1299, 1861) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3811) [M::mem_pestat] mean and std.dev: (1095.08, 644.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4786) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 898.982 CPU sec, 39.861 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (82, 666788, 34, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (49, 83, 138) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 316) [M::mem_pestat] mean and std.dev: (95.03, 66.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 405) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.43, 75.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (42, 99, 1731) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5109) [M::mem_pestat] mean and std.dev: (934.28, 1497.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6925) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (755, 1303, 1676) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3518) [M::mem_pestat] mean and std.dev: (1147.79, 678.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4439) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 903.051 CPU sec, 50.067 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (75, 667309, 32, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (43, 72, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (91.33, 73.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 659) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.50, 75.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 59, 651) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1897) [M::mem_pestat] mean and std.dev: (180.63, 384.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2520) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 901.631 CPU sec, 40.157 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (78, 667286, 31, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (34, 58, 156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400) [M::mem_pestat] mean and std.dev: (81.86, 73.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 522) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.41, 75.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (54, 89, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 825) [M::mem_pestat] mean and std.dev: (122.85, 168.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1082) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (704, 1016, 1305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2507) [M::mem_pestat] mean and std.dev: (879.92, 366.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3108) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 899.193 CPU sec, 51.720 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (64, 666586, 22, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (38, 83, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (94.93, 74.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.31, 75.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 62, 156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 426) [M::mem_pestat] mean and std.dev: (58.47, 47.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 561) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (230, 824, 2295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6425) [M::mem_pestat] mean and std.dev: (1104.58, 1255.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8490) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 901.894 CPU sec, 40.049 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (75, 667356, 34, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (46, 77, 156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 376) [M::mem_pestat] mean and std.dev: (94.73, 64.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 486) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.35, 75.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 85, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 571) [M::mem_pestat] mean and std.dev: (66.50, 60.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 755) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (538, 986, 1456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3292) [M::mem_pestat] mean and std.dev: (999.25, 695.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4210) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 903.179 CPU sec, 50.867 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (84, 666683, 33, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (43, 72, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 511) [M::mem_pestat] mean and std.dev: (113.44, 97.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 667) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.52, 75.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (52, 77, 155) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 361) [M::mem_pestat] mean and std.dev: (66.77, 37.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 464) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (651, 1554, 3196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8286) [M::mem_pestat] mean and std.dev: (2142.31, 2130.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10831) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 906.422 CPU sec, 40.261 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (80, 666607, 30, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (47, 77, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 422) [M::mem_pestat] mean and std.dev: (84.25, 64.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 547) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.47, 75.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (56, 115, 468) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1292) [M::mem_pestat] mean and std.dev: (136.46, 167.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1704) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (539, 969, 2144) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5354) [M::mem_pestat] mean and std.dev: (1236.00, 1070.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6959) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 912.772 CPU sec, 53.923 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (62, 667551, 30, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (47, 80, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 431) [M::mem_pestat] mean and std.dev: (99.20, 75.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 559) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.46, 75.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 42, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937) [M::mem_pestat] mean and std.dev: (56.13, 67.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1242) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (756, 1126, 2279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5325) [M::mem_pestat] mean and std.dev: (1525.33, 1153.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6848) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 909.325 CPU sec, 40.716 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (88, 666836, 31, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (45, 71, 149) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 357) [M::mem_pestat] mean and std.dev: (88.54, 61.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.46, 75.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 67, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (66.71, 53.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 612) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 914.950 CPU sec, 53.478 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (66, 666152, 26, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (39, 58, 116) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 270) [M::mem_pestat] mean and std.dev: (71.78, 50.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 347) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.48, 75.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (43, 238, 1451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4267) [M::mem_pestat] mean and std.dev: (840.00, 1131.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5675) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 916.592 CPU sec, 53.477 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (73, 666990, 24, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (42, 73, 148) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 360) [M::mem_pestat] mean and std.dev: (85.19, 58.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 466) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.27, 75.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 112, 3230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9634) [M::mem_pestat] mean and std.dev: (1625.50, 2474.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12836) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (873, 1060, 2045) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4389) [M::mem_pestat] mean and std.dev: (1305.08, 950.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5561) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 909.070 CPU sec, 40.493 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (76, 666819, 26, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (42, 71, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 399) [M::mem_pestat] mean and std.dev: (89.22, 67.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 518) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 262, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (11, 521) [M::mem_pestat] mean and std.dev: (269.12, 75.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 90, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 989) [M::mem_pestat] mean and std.dev: (104.48, 123.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1307) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (618, 940, 2481) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6207) [M::mem_pestat] mean and std.dev: (1390.19, 1219.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8070) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 910.642 CPU sec, 53.262 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (68, 667295, 26, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (41, 70, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 434) [M::mem_pestat] mean and std.dev: (85.45, 56.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 565) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.37, 75.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (53, 129, 1281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3737) [M::mem_pestat] mean and std.dev: (686.56, 1111.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5133) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 906.520 CPU sec, 40.378 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (63, 666715, 33, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (47, 100, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (111.81, 83.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.32, 75.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (50, 93, 1488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4364) [M::mem_pestat] mean and std.dev: (515.37, 927.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5802) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (866, 1542, 3220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7928) [M::mem_pestat] mean and std.dev: (1828.50, 1418.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10282) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 909.185 CPU sec, 53.646 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (74, 667059, 32, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (44, 74, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 440) [M::mem_pestat] mean and std.dev: (92.48, 73.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 572) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.46, 75.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 93, 1350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3984) [M::mem_pestat] mean and std.dev: (354.59, 773.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5301) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (707, 1102, 1572) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3302) [M::mem_pestat] mean and std.dev: (1036.84, 638.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4167) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 906.883 CPU sec, 40.430 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (77, 667236, 30, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (41, 67, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 551) [M::mem_pestat] mean and std.dev: (105.03, 91.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 721) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.54, 75.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 124, 595) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1735) [M::mem_pestat] mean and std.dev: (112.22, 146.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2305) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (509, 884, 1693) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4061) [M::mem_pestat] mean and std.dev: (895.78, 713.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5245) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 905.282 CPU sec, 52.738 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (72, 666965, 34, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (38, 58, 147) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 365) [M::mem_pestat] mean and std.dev: (81.21, 69.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 474) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.28, 75.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 106, 499) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1435) [M::mem_pestat] mean and std.dev: (216.03, 310.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1903) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 909.386 CPU sec, 40.549 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (79, 667205, 29, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (42, 82, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 447) [M::mem_pestat] mean and std.dev: (95.77, 74.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 582) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.37, 75.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 138, 2408) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7160) [M::mem_pestat] mean and std.dev: (948.07, 1382.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9536) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (541, 1228, 2155) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5383) [M::mem_pestat] mean and std.dev: (1061.00, 819.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6997) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 910.000 CPU sec, 53.479 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (68, 666401, 30, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (40, 80, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 436) [M::mem_pestat] mean and std.dev: (102.15, 77.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 568) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (11, 521) [M::mem_pestat] mean and std.dev: (269.15, 75.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (49, 103, 400) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1102) [M::mem_pestat] mean and std.dev: (131.36, 167.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1453) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 911.649 CPU sec, 40.564 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (58, 667003, 24, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (38, 91, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 494) [M::mem_pestat] mean and std.dev: (120.21, 108.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 646) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.37, 75.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (44, 133, 335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917) [M::mem_pestat] mean and std.dev: (111.70, 114.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1208) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (515, 1083, 1741) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4193) [M::mem_pestat] mean and std.dev: (1217.93, 855.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5419) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 909.122 CPU sec, 52.270 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (71, 666725, 15, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (46, 99, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 562) [M::mem_pestat] mean and std.dev: (111.97, 104.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 734) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.42, 75.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (38, 65, 613) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1763) [M::mem_pestat] mean and std.dev: (159.85, 245.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2338) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (774, 1099, 1676) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3480) [M::mem_pestat] mean and std.dev: (1325.47, 853.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4738) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 907.600 CPU sec, 40.353 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (77, 667357, 32, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (39, 69, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 684) [M::mem_pestat] mean and std.dev: (114.07, 122.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 899) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.49, 75.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 67, 126) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 318) [M::mem_pestat] mean and std.dev: (57.35, 42.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 414) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (503, 762, 1393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3173) [M::mem_pestat] mean and std.dev: (895.38, 548.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4063) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 906.211 CPU sec, 53.040 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (63, 666929, 34, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (46, 77, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 718) [M::mem_pestat] mean and std.dev: (114.69, 121.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 942) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.56, 75.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 67, 158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 430) [M::mem_pestat] mean and std.dev: (60.74, 53.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 566) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (766, 937, 1441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2791) [M::mem_pestat] mean and std.dev: (1067.50, 640.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3630) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 904.876 CPU sec, 40.318 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (70, 667317, 20, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (46, 85, 138) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 322) [M::mem_pestat] mean and std.dev: (78.68, 46.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 414) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.45, 75.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (78, 182, 2147) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6285) [M::mem_pestat] mean and std.dev: (1141.20, 1705.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8354) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (973, 1808, 1923) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3823) [M::mem_pestat] mean and std.dev: (1422.10, 636.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4773) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 907.691 CPU sec, 50.192 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (78, 665987, 25, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (40, 69, 116) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 268) [M::mem_pestat] mean and std.dev: (76.51, 55.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 344) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.53, 75.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (51, 106, 1472) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4314) [M::mem_pestat] mean and std.dev: (513.64, 986.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5735) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 912.854 CPU sec, 51.789 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (81, 666941, 29, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (41, 55, 141) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 341) [M::mem_pestat] mean and std.dev: (76.47, 53.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 441) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.30, 75.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 63, 117) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 287) [M::mem_pestat] mean and std.dev: (64.89, 43.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 372) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (803, 1183, 2496) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5882) [M::mem_pestat] mean and std.dev: (1341.64, 834.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7575) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 910.224 CPU sec, 40.543 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (92, 667175, 35, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (36, 60, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 456) [M::mem_pestat] mean and std.dev: (80.91, 67.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 596) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.38, 75.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (41, 61, 457) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1289) [M::mem_pestat] mean and std.dev: (129.83, 174.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1705) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (693, 935, 1817) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4065) [M::mem_pestat] mean and std.dev: (1147.44, 744.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5189) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 916.196 CPU sec, 53.541 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (75, 667127, 29, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (41, 68, 156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 386) [M::mem_pestat] mean and std.dev: (94.23, 78.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 501) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.38, 75.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (60, 149, 1579) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4617) [M::mem_pestat] mean and std.dev: (996.28, 1656.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7622) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (700, 1026, 1518) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3154) [M::mem_pestat] mean and std.dev: (1006.25, 467.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3972) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 914.064 CPU sec, 40.773 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (65, 667149, 25, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (50, 81, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 779) [M::mem_pestat] mean and std.dev: (128.07, 126.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1022) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.45, 75.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 64, 143) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 389) [M::mem_pestat] mean and std.dev: (58.57, 45.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 512) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (185, 932, 1535) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4235) [M::mem_pestat] mean and std.dev: (866.00, 725.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5585) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 904.892 CPU sec, 51.337 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (69, 667022, 22, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (38, 58, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 428) [M::mem_pestat] mean and std.dev: (84.13, 74.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 558) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (11, 521) [M::mem_pestat] mean and std.dev: (269.21, 75.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 58, 580) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1706) [M::mem_pestat] mean and std.dev: (130.28, 221.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2269) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (207, 736, 1799) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4983) [M::mem_pestat] mean and std.dev: (857.80, 818.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6575) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 907.509 CPU sec, 40.420 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (58, 666431, 27, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (43, 65, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 445) [M::mem_pestat] mean and std.dev: (82.79, 71.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 579) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.53, 75.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 92, 983) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2895) [M::mem_pestat] mean and std.dev: (391.72, 714.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3851) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (253, 871, 1041) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2617) [M::mem_pestat] mean and std.dev: (624.67, 355.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3405) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 916.060 CPU sec, 54.409 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (77, 667331, 26, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (44, 85, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 431) [M::mem_pestat] mean and std.dev: (101.87, 80.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 560) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.25, 75.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 63, 134) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 362) [M::mem_pestat] mean and std.dev: (59.73, 45.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 476) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (558, 887, 1122) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2250) [M::mem_pestat] mean and std.dev: (800.00, 363.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2814) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 907.142 CPU sec, 40.323 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (75, 666902, 30, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (45, 79, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (99.85, 83.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 633) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.37, 75.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (47, 132, 1686) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4964) [M::mem_pestat] mean and std.dev: (870.50, 1520.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6954) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (189, 590, 1689) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4689) [M::mem_pestat] mean and std.dev: (577.55, 496.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6189) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 913.842 CPU sec, 54.265 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (72, 666588, 16, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (41, 68, 131) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 311) [M::mem_pestat] mean and std.dev: (89.03, 68.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 401) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.43, 75.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (40, 100, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 433) [M::mem_pestat] mean and std.dev: (74.08, 52.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 564) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (310, 1100, 1566) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4078) [M::mem_pestat] mean and std.dev: (954.92, 573.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5334) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 904.863 CPU sec, 40.287 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (68, 667129, 29, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (36, 60, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 447) [M::mem_pestat] mean and std.dev: (99.08, 87.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 584) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.38, 75.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 58, 553) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1617) [M::mem_pestat] mean and std.dev: (74.96, 115.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2149) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (632, 765, 1541) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3359) [M::mem_pestat] mean and std.dev: (972.69, 763.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4268) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 906.479 CPU sec, 53.559 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (72, 666707, 29, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (42, 71, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 495) [M::mem_pestat] mean and std.dev: (91.82, 88.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 646) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.44, 75.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 97, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 779) [M::mem_pestat] mean and std.dev: (122.40, 152.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1027) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (633, 919, 1577) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3465) [M::mem_pestat] mean and std.dev: (902.21, 677.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4409) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 909.581 CPU sec, 40.432 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (74, 667111, 25, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (45, 78, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 423) [M::mem_pestat] mean and std.dev: (103.59, 81.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 549) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.45, 75.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (57, 140, 2893) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8565) [M::mem_pestat] mean and std.dev: (1311.20, 2111.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11401) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (590, 850, 1748) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4064) [M::mem_pestat] mean and std.dev: (940.44, 572.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5222) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 906.085 CPU sec, 54.015 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (76, 666408, 26, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (35, 56, 137) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 341) [M::mem_pestat] mean and std.dev: (78.73, 64.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 443) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.61, 75.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 104, 147) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 397) [M::mem_pestat] mean and std.dev: (86.68, 83.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 522) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (689, 853, 1102) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1928) [M::mem_pestat] mean and std.dev: (809.67, 407.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2438) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 911.483 CPU sec, 40.603 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (63, 666450, 23, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 89, 155) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 355) [M::mem_pestat] mean and std.dev: (96.72, 69.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 455) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.23, 75.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (60, 114, 1250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3630) [M::mem_pestat] mean and std.dev: (324.40, 468.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4820) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 900.854 CPU sec, 48.202 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (91, 666759, 38, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (37, 50, 122) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 292) [M::mem_pestat] mean and std.dev: (68.86, 54.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 377) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.36, 75.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 88, 483) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1377) [M::mem_pestat] mean and std.dev: (188.61, 265.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1824) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (633, 816, 2151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5187) [M::mem_pestat] mean and std.dev: (1273.43, 1035.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6705) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 909.338 CPU sec, 52.490 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (59, 666493, 41, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (38, 104, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 431) [M::mem_pestat] mean and std.dev: (98.49, 67.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 562) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.31, 75.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 102, 803) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2341) [M::mem_pestat] mean and std.dev: (242.37, 480.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3110) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (576, 856, 958) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1722) [M::mem_pestat] mean and std.dev: (739.89, 305.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2104) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 905.414 CPU sec, 40.222 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (73, 666870, 33, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (40, 72, 156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388) [M::mem_pestat] mean and std.dev: (76.56, 53.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 504) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.43, 75.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (41, 117, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (87.15, 57.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 617) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (342, 1030, 1348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3360) [M::mem_pestat] mean and std.dev: (1096.64, 909.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4736) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 914.104 CPU sec, 53.473 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (73, 667096, 23, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (39, 73, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 528) [M::mem_pestat] mean and std.dev: (114.35, 101.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 691) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.29, 75.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 505, 1750) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5178) [M::mem_pestat] mean and std.dev: (654.80, 822.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6892) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (918, 1051, 2250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4914) [M::mem_pestat] mean and std.dev: (1276.10, 596.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6246) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 906.433 CPU sec, 40.302 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (70, 666949, 22, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (39, 64, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 435) [M::mem_pestat] mean and std.dev: (94.37, 78.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 567) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.53, 75.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (59, 127, 626) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1760) [M::mem_pestat] mean and std.dev: (279.95, 449.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2327) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (556, 855, 1285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2743) [M::mem_pestat] mean and std.dev: (853.91, 514.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3472) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 906.752 CPU sec, 53.066 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (73, 666099, 31, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (39, 71, 151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 375) [M::mem_pestat] mean and std.dev: (88.94, 66.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 487) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.34, 75.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (70, 114, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 712) [M::mem_pestat] mean and std.dev: (89.25, 60.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 926) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (557, 1128, 1800) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4286) [M::mem_pestat] mean and std.dev: (1337.29, 1019.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5529) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 908.997 CPU sec, 40.340 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (82, 666577, 26, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (37, 62, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 433) [M::mem_pestat] mean and std.dev: (81.96, 71.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 565) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.42, 75.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 62, 388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1098) [M::mem_pestat] mean and std.dev: (130.73, 180.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1453) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (731, 991, 1793) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3917) [M::mem_pestat] mean and std.dev: (1050.42, 604.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4979) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 907.752 CPU sec, 53.484 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (76, 667240, 36, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (44, 79, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 524) [M::mem_pestat] mean and std.dev: (106.68, 95.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 684) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.35, 75.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (48, 115, 1276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3732) [M::mem_pestat] mean and std.dev: (414.59, 760.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4960) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (286, 699, 1106) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2746) [M::mem_pestat] mean and std.dev: (554.50, 344.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3566) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 909.820 CPU sec, 40.564 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (63, 667157, 34, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (37, 66, 118) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (82.37, 65.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 361) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.50, 75.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (40, 81, 867) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2521) [M::mem_pestat] mean and std.dev: (304.32, 521.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3348) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (589, 1200, 1391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2995) [M::mem_pestat] mean and std.dev: (1145.40, 601.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3797) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 909.565 CPU sec, 53.838 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (77, 666809, 30, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (43, 75, 148) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 358) [M::mem_pestat] mean and std.dev: (91.50, 66.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 463) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.36, 75.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 98, 868) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2544) [M::mem_pestat] mean and std.dev: (241.88, 473.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3382) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (841, 944, 1436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2626) [M::mem_pestat] mean and std.dev: (915.73, 484.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3221) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 912.489 CPU sec, 40.580 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (84, 667519, 27, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (45, 101, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 549) [M::mem_pestat] mean and std.dev: (126.90, 112.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 717) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.47, 75.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 47, 130) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 340) [M::mem_pestat] mean and std.dev: (64.75, 70.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 445) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 907.119 CPU sec, 52.718 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (82, 666742, 31, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (42, 61, 121) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 279) [M::mem_pestat] mean and std.dev: (80.45, 60.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 358) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (216, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (12, 522) [M::mem_pestat] mean and std.dev: (269.44, 75.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 624) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (41, 112, 1576) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4646) [M::mem_pestat] mean and std.dev: (832.97, 1405.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6456) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (475, 851, 1042) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2176) [M::mem_pestat] mean and std.dev: (858.79, 408.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2743) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 908.920 CPU sec, 40.514 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (72, 667131, 27, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (44, 71, 146) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 350) [M::mem_pestat] mean and std.dev: (82.81, 59.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 452) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.33, 75.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (48, 156, 2022) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5970) [M::mem_pestat] mean and std.dev: (948.40, 1285.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7944) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 914.307 CPU sec, 53.069 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (78, 666901, 30, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (42, 70, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (94.67, 74.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 542) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.27, 75.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 87, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 434) [M::mem_pestat] mean and std.dev: (61.83, 49.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 568) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 910.986 CPU sec, 40.511 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (82, 667421, 31, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (46, 80, 432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1204) [M::mem_pestat] mean and std.dev: (178.83, 250.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1590) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (216, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (12, 522) [M::mem_pestat] mean and std.dev: (269.50, 75.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 624) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (46, 89, 941) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2731) [M::mem_pestat] mean and std.dev: (229.88, 470.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3626) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 901.153 CPU sec, 45.560 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (81, 666960, 34, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (37, 66, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 544) [M::mem_pestat] mean and std.dev: (98.94, 100.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 713) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.35, 75.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 134, 1900) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5646) [M::mem_pestat] mean and std.dev: (918.09, 1388.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7519) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (638, 1811, 2339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5741) [M::mem_pestat] mean and std.dev: (1755.00, 1508.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7789) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 910.153 CPU sec, 53.594 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (68, 667209, 32, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (41, 85, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (92.26, 79.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 605) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.31, 75.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 59, 1207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3567) [M::mem_pestat] mean and std.dev: (223.70, 416.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4747) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (720, 943, 1493) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3039) [M::mem_pestat] mean and std.dev: (1106.83, 525.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3812) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 908.020 CPU sec, 40.478 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (80, 666766, 32, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (44, 76, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 395) [M::mem_pestat] mean and std.dev: (91.05, 64.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 512) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.46, 75.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (70, 113, 938) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2674) [M::mem_pestat] mean and std.dev: (318.04, 562.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3542) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (601, 1064, 2363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5887) [M::mem_pestat] mean and std.dev: (1261.29, 1071.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7649) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 911.152 CPU sec, 52.946 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (68, 666680, 26, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (46, 83, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (106.63, 97.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 654) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.37, 75.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (54, 162, 569) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1599) [M::mem_pestat] mean and std.dev: (135.20, 131.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2114) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (689, 1407, 2261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5405) [M::mem_pestat] mean and std.dev: (1632.18, 1297.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6977) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 906.648 CPU sec, 40.599 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (70, 667313, 27, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (37, 56, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (87.13, 81.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 633) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.51, 75.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 67, 150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (74.88, 66.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 552) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 906.326 CPU sec, 52.027 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (73, 666956, 35, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (35, 68, 140) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 350) [M::mem_pestat] mean and std.dev: (85.01, 67.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 455) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.53, 75.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (49, 111, 2511) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7435) [M::mem_pestat] mean and std.dev: (1040.36, 1784.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9897) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (745, 1026, 2130) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4900) [M::mem_pestat] mean and std.dev: (1213.54, 729.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6285) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 900.905 CPU sec, 40.202 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (77, 667253, 23, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (39, 91, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 540) [M::mem_pestat] mean and std.dev: (113.32, 95.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 707) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.46, 75.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (49, 87, 2742) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8128) [M::mem_pestat] mean and std.dev: (992.57, 1607.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10821) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (662, 1011, 1231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2369) [M::mem_pestat] mean and std.dev: (766.00, 415.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2938) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 911.185 CPU sec, 53.668 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 666842, 31, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (43, 67, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 577) [M::mem_pestat] mean and std.dev: (100.39, 97.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 755) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.38, 75.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (40, 128, 1221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3583) [M::mem_pestat] mean and std.dev: (344.67, 557.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4764) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (545, 904, 1558) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3584) [M::mem_pestat] mean and std.dev: (967.21, 758.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4597) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 904.918 CPU sec, 40.424 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (80, 666671, 32, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (45, 72, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573) [M::mem_pestat] mean and std.dev: (95.97, 88.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 749) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.58, 75.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (47, 120, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1169) [M::mem_pestat] mean and std.dev: (181.20, 202.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1543) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 908.848 CPU sec, 52.601 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (76, 666580, 33, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (39, 68, 153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 381) [M::mem_pestat] mean and std.dev: (83.96, 61.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 495) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.36, 75.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (40, 101, 1074) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3142) [M::mem_pestat] mean and std.dev: (147.50, 225.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4176) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (344, 1094, 2519) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6869) [M::mem_pestat] mean and std.dev: (1291.38, 1208.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9044) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 916.281 CPU sec, 40.763 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (74, 666689, 24, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (37, 68, 140) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 346) [M::mem_pestat] mean and std.dev: (80.78, 62.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 449) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.33, 75.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (41, 121, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 449) [M::mem_pestat] mean and std.dev: (79.05, 57.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (810, 956, 1336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2388) [M::mem_pestat] mean and std.dev: (979.57, 365.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2914) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 910.532 CPU sec, 51.962 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (63, 667180, 26, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (38, 64, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 437) [M::mem_pestat] mean and std.dev: (86.06, 77.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 570) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.34, 75.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 97, 1972) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5864) [M::mem_pestat] mean and std.dev: (1028.19, 1460.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7810) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (606, 1074, 1610) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3618) [M::mem_pestat] mean and std.dev: (921.50, 572.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4622) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 907.684 CPU sec, 40.315 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (82, 667238, 24, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (42, 65, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 441) [M::mem_pestat] mean and std.dev: (91.17, 76.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 574) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.43, 75.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 65, 125) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 307) [M::mem_pestat] mean and std.dev: (68.77, 45.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 398) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (384, 645, 1740) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4452) [M::mem_pestat] mean and std.dev: (963.46, 1000.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5808) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 906.475 CPU sec, 53.712 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (100, 666886, 31, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (47, 97, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 545) [M::mem_pestat] mean and std.dev: (103.72, 85.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 711) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.54, 75.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 50, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (74.30, 88.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 628) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 905.875 CPU sec, 40.279 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (100, 667319, 30, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (39, 57, 150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 372) [M::mem_pestat] mean and std.dev: (89.78, 75.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 483) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.34, 75.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 48, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (62.67, 69.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (572, 1068, 2435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6161) [M::mem_pestat] mean and std.dev: (1335.60, 938.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8024) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 897.420 CPU sec, 42.551 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (74, 666950, 22, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (43, 86, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403) [M::mem_pestat] mean and std.dev: (95.31, 67.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 523) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.51, 75.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 104, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 548) [M::mem_pestat] mean and std.dev: (64.82, 58.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 722) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 912.072 CPU sec, 53.928 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (64, 666761, 24, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (38, 59, 145) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 359) [M::mem_pestat] mean and std.dev: (87.52, 71.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 466) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.48, 75.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 51, 126) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 330) [M::mem_pestat] mean and std.dev: (47.85, 36.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 432) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (722, 1103, 2204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5168) [M::mem_pestat] mean and std.dev: (1165.27, 819.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6650) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 905.897 CPU sec, 40.204 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (73, 666768, 26, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (38, 73, 136) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 332) [M::mem_pestat] mean and std.dev: (94.93, 69.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 430) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (11, 521) [M::mem_pestat] mean and std.dev: (269.16, 75.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (42, 107, 1694) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4998) [M::mem_pestat] mean and std.dev: (977.69, 1411.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6650) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (565, 1356, 2117) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5221) [M::mem_pestat] mean and std.dev: (1188.06, 783.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6773) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 909.156 CPU sec, 52.957 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (72, 667379, 17, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (40, 72, 155) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 385) [M::mem_pestat] mean and std.dev: (87.43, 68.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 500) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (216, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (12, 522) [M::mem_pestat] mean and std.dev: (269.39, 75.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 624) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 109, 3860) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11506) [M::mem_pestat] mean and std.dev: (1605.06, 2588.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15329) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 901.989 CPU sec, 40.154 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (78, 666469, 38, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (36, 71, 155) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 393) [M::mem_pestat] mean and std.dev: (90.23, 74.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 512) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.48, 75.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 49, 112) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 288) [M::mem_pestat] mean and std.dev: (42.31, 30.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 376) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (601, 1003, 1711) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3931) [M::mem_pestat] mean and std.dev: (1149.29, 959.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5041) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 910.056 CPU sec, 53.122 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (70, 667238, 25, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (43, 73, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (99.75, 84.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 531) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.49, 75.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 78, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907) [M::mem_pestat] mean and std.dev: (78.45, 77.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1198) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (758, 1207, 2285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5339) [M::mem_pestat] mean and std.dev: (1279.58, 736.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6866) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 910.356 CPU sec, 40.466 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (66, 667402, 31, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (41, 72, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 398) [M::mem_pestat] mean and std.dev: (89.08, 68.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 517) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.54, 75.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 99, 1602) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4732) [M::mem_pestat] mean and std.dev: (635.38, 1148.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6297) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (532, 1347, 1945) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4771) [M::mem_pestat] mean and std.dev: (1300.07, 892.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6184) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 909.425 CPU sec, 52.792 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 666918, 35, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (41, 91, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (94.19, 74.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 533) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.39, 75.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 52, 752) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2218) [M::mem_pestat] mean and std.dev: (171.10, 337.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2951) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (268, 586, 1377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3595) [M::mem_pestat] mean and std.dev: (712.42, 581.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4704) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 909.971 CPU sec, 40.452 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (75, 667360, 20, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (41, 67, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 419) [M::mem_pestat] mean and std.dev: (102.54, 83.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 545) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.40, 75.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (39, 108, 1934) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5724) [M::mem_pestat] mean and std.dev: (873.40, 1281.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7619) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (479, 721, 802) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1448) [M::mem_pestat] mean and std.dev: (615.67, 269.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1771) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 907.769 CPU sec, 53.081 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (70, 667380, 32, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (39, 70, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (92.11, 71.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.54, 75.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 89, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 959) [M::mem_pestat] mean and std.dev: (121.78, 185.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1268) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (694, 1114, 2604) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6424) [M::mem_pestat] mean and std.dev: (1487.55, 1174.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8334) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 901.622 CPU sec, 40.058 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (80, 667225, 42, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (47, 118, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716) [M::mem_pestat] mean and std.dev: (147.96, 144.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 939) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.29, 75.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 126, 1542) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4570) [M::mem_pestat] mean and std.dev: (633.67, 1124.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6084) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (439, 975, 1241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2845) [M::mem_pestat] mean and std.dev: (1010.10, 743.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3983) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 914.428 CPU sec, 54.639 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (87, 667513, 31, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (40, 70, 154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 382) [M::mem_pestat] mean and std.dev: (78.57, 59.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 496) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.43, 75.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 104, 377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1059) [M::mem_pestat] mean and std.dev: (94.25, 93.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1400) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (182, 1129, 1344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3668) [M::mem_pestat] mean and std.dev: (1066.80, 950.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4869) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 903.669 CPU sec, 40.329 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (74, 666504, 30, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (33, 54, 114) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 276) [M::mem_pestat] mean and std.dev: (69.63, 56.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 357) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.53, 75.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (89, 148, 1140) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3242) [M::mem_pestat] mean and std.dev: (431.11, 666.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4293) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (702, 826, 1658) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3570) [M::mem_pestat] mean and std.dev: (993.87, 789.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4526) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 916.936 CPU sec, 54.151 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (85, 667362, 32, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (43, 66, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (92.81, 77.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 647) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.49, 75.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (65, 112, 1789) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5237) [M::mem_pestat] mean and std.dev: (932.94, 1493.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6961) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (490, 892, 1533) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3619) [M::mem_pestat] mean and std.dev: (1089.83, 905.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4713) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 909.876 CPU sec, 40.714 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (83, 667537, 33, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (43, 68, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (102.22, 83.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 611) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.39, 75.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 112, 1080) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3184) [M::mem_pestat] mean and std.dev: (296.93, 474.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4236) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (869, 988, 1426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2540) [M::mem_pestat] mean and std.dev: (1009.67, 439.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3097) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 896.016 CPU sec, 41.062 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (83, 666583, 22, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (45, 77, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 426) [M::mem_pestat] mean and std.dev: (92.19, 69.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 553) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.43, 75.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (44, 89, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1265) [M::mem_pestat] mean and std.dev: (163.21, 225.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1672) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (900, 1306, 1654) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3162) [M::mem_pestat] mean and std.dev: (1115.56, 674.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3916) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 913.066 CPU sec, 53.192 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (66, 666851, 22, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (41, 69, 142) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 344) [M::mem_pestat] mean and std.dev: (87.78, 70.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 445) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.26, 75.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 128, 159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403) [M::mem_pestat] mean and std.dev: (78.35, 51.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 525) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 905.355 CPU sec, 40.231 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (85, 666508, 41, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (41, 91, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635) [M::mem_pestat] mean and std.dev: (115.23, 115.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 833) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.36, 75.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 96, 2240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6652) [M::mem_pestat] mean and std.dev: (963.10, 1674.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8858) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 914.095 CPU sec, 53.241 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (82, 666261, 20, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (39, 79, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (96.52, 78.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 615) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.39, 75.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 50, 157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 441) [M::mem_pestat] mean and std.dev: (65.37, 62.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 583) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (552, 1740, 3089) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8163) [M::mem_pestat] mean and std.dev: (2244.42, 2243.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11219) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 908.389 CPU sec, 40.413 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (82, 666694, 35, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (36, 77, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 429) [M::mem_pestat] mean and std.dev: (89.71, 74.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 560) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.40, 75.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 134, 1457) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4301) [M::mem_pestat] mean and std.dev: (684.12, 1038.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5723) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (632, 1149, 1966) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4634) [M::mem_pestat] mean and std.dev: (1351.19, 1153.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5968) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 909.611 CPU sec, 53.912 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (66, 667525, 24, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (42, 82, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 453) [M::mem_pestat] mean and std.dev: (81.66, 60.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.48, 75.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 84, 129) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 321) [M::mem_pestat] mean and std.dev: (77.48, 64.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 417) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1092, 1471, 3820) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9276) [M::mem_pestat] mean and std.dev: (2222.79, 1831.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12004) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 905.536 CPU sec, 40.489 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (74, 667051, 26, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (45, 104, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 558) [M::mem_pestat] mean and std.dev: (106.72, 87.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 729) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.41, 75.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 47, 415) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1195) [M::mem_pestat] mean and std.dev: (120.38, 252.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1585) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (695, 1023, 1630) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3500) [M::mem_pestat] mean and std.dev: (1115.87, 733.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4435) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 910.234 CPU sec, 53.069 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (74, 667163, 28, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (37, 60, 130) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 316) [M::mem_pestat] mean and std.dev: (81.15, 68.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 409) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 262, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.26, 75.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 79, 604) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1746) [M::mem_pestat] mean and std.dev: (165.71, 292.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2317) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (752, 913, 1531) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3089) [M::mem_pestat] mean and std.dev: (1143.31, 594.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3868) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 908.468 CPU sec, 40.459 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (74, 667355, 24, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (43, 74, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 508) [M::mem_pestat] mean and std.dev: (99.44, 88.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 663) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.24, 75.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (40, 73, 127) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 301) [M::mem_pestat] mean and std.dev: (68.71, 51.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 388) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (263, 715, 2996) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8462) [M::mem_pestat] mean and std.dev: (1815.00, 2084.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11195) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 905.901 CPU sec, 53.263 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (69, 666841, 29, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (44, 76, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (106.41, 98.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 668) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.50, 75.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (58, 137, 1530) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4474) [M::mem_pestat] mean and std.dev: (632.00, 968.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5946) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (620, 1110, 1975) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4685) [M::mem_pestat] mean and std.dev: (1255.92, 1170.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6040) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 915.458 CPU sec, 40.803 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (81, 666929, 33, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (43, 78, 156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 382) [M::mem_pestat] mean and std.dev: (93.63, 62.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 495) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.45, 75.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (45, 102, 1289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3777) [M::mem_pestat] mean and std.dev: (505.80, 996.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5021) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 908.588 CPU sec, 51.548 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (78, 667021, 35, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (35, 65, 136) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 338) [M::mem_pestat] mean and std.dev: (77.99, 60.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 439) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.44, 75.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 87, 2434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7236) [M::mem_pestat] mean and std.dev: (884.48, 1535.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9637) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (765, 953, 1248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2214) [M::mem_pestat] mean and std.dev: (960.50, 380.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2697) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 904.769 CPU sec, 39.690 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (79, 666732, 33, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (44, 68, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 413) [M::mem_pestat] mean and std.dev: (91.45, 71.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 536) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.39, 75.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 106, 1975) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5883) [M::mem_pestat] mean and std.dev: (743.80, 1285.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7837) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (259, 1242, 2001) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5485) [M::mem_pestat] mean and std.dev: (1097.31, 720.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7227) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 914.563 CPU sec, 52.893 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (68, 666610, 30, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (49, 96, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 547) [M::mem_pestat] mean and std.dev: (103.63, 81.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 713) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.36, 75.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 182, 1541) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4591) [M::mem_pestat] mean and std.dev: (549.78, 826.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6116) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (300, 523, 1795) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4785) [M::mem_pestat] mean and std.dev: (977.57, 1283.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6280) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 911.199 CPU sec, 40.443 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (81, 666900, 28, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (39, 57, 124) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 294) [M::mem_pestat] mean and std.dev: (72.54, 54.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 379) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.30, 75.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 50, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628) [M::mem_pestat] mean and std.dev: (70.96, 89.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 830) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (535, 893, 1585) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3685) [M::mem_pestat] mean and std.dev: (998.96, 713.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4735) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 906.087 CPU sec, 53.307 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (88, 666909, 27, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (43, 61, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (85.05, 70.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 611) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.34, 75.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 69, 728) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2122) [M::mem_pestat] mean and std.dev: (260.70, 515.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2819) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (724, 1681, 1975) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4477) [M::mem_pestat] mean and std.dev: (1365.56, 729.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5728) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 908.253 CPU sec, 40.461 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (77, 667333, 23, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (34, 85, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (97.28, 77.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 630) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.39, 75.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (71, 108, 454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1220) [M::mem_pestat] mean and std.dev: (109.28, 96.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1603) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (774, 1090, 2322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5418) [M::mem_pestat] mean and std.dev: (1327.29, 820.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6966) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 905.885 CPU sec, 52.522 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (72, 667526, 37, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (44, 71, 150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 362) [M::mem_pestat] mean and std.dev: (82.72, 56.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 468) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.36, 75.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 54, 2875) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8581) [M::mem_pestat] mean and std.dev: (1442.16, 2482.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11434) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (656, 870, 1781) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4031) [M::mem_pestat] mean and std.dev: (1009.73, 679.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5156) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 904.656 CPU sec, 40.161 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (61, 666482, 29, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (40, 84, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 538) [M::mem_pestat] mean and std.dev: (112.49, 111.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 704) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.28, 75.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 78, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 533) [M::mem_pestat] mean and std.dev: (71.75, 62.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 700) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (774, 1184, 1424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2724) [M::mem_pestat] mean and std.dev: (971.77, 397.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3374) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 909.492 CPU sec, 52.745 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (91, 667193, 21, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (35, 64, 157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (82.98, 71.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 523) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.48, 75.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 95, 349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 973) [M::mem_pestat] mean and std.dev: (98.06, 100.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1285) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (984, 1112, 2760) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6312) [M::mem_pestat] mean and std.dev: (1585.64, 1137.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8088) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 904.181 CPU sec, 40.185 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (69, 665849, 29, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (45, 66, 150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 360) [M::mem_pestat] mean and std.dev: (83.48, 59.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 465) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.37, 75.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 108, 4247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12675) [M::mem_pestat] mean and std.dev: (1569.21, 2451.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16889) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 909.395 CPU sec, 53.598 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (71, 666800, 28, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (44, 84, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488) [M::mem_pestat] mean and std.dev: (96.21, 83.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 636) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.39, 75.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 135, 2693) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8009) [M::mem_pestat] mean and std.dev: (1171.59, 1805.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10667) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (616, 843, 1964) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4660) [M::mem_pestat] mean and std.dev: (1032.50, 701.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6008) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 908.240 CPU sec, 40.521 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (62, 666769, 35, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (47, 64, 145) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 341) [M::mem_pestat] mean and std.dev: (76.54, 52.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 439) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.28, 75.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 69, 138) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 358) [M::mem_pestat] mean and std.dev: (72.88, 54.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 468) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (342, 1153, 2429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6603) [M::mem_pestat] mean and std.dev: (1294.54, 1120.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8690) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 910.983 CPU sec, 53.533 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (70, 666447, 33, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (44, 85, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 551) [M::mem_pestat] mean and std.dev: (101.76, 84.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 720) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.41, 75.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (57, 129, 1363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3975) [M::mem_pestat] mean and std.dev: (366.45, 618.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5281) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 904.814 CPU sec, 40.358 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (68, 667276, 25, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (44, 74, 147) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 353) [M::mem_pestat] mean and std.dev: (88.82, 60.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 456) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.55, 75.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (45, 124, 3122) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9276) [M::mem_pestat] mean and std.dev: (1324.96, 2091.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12353) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (798, 966, 1901) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4107) [M::mem_pestat] mean and std.dev: (1152.31, 589.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5210) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 906.977 CPU sec, 52.950 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (81, 666948, 31, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (40, 79, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 415) [M::mem_pestat] mean and std.dev: (96.41, 75.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 540) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (11, 521) [M::mem_pestat] mean and std.dev: (269.15, 75.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 57, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462) [M::mem_pestat] mean and std.dev: (63.60, 56.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 609) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (211, 928, 1984) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5530) [M::mem_pestat] mean and std.dev: (923.73, 771.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7303) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 904.159 CPU sec, 40.165 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (63, 666966, 21, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (37, 77, 146) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 364) [M::mem_pestat] mean and std.dev: (85.34, 60.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 473) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.46, 75.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 57, 1617) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4787) [M::mem_pestat] mean and std.dev: (816.10, 1288.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6372) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (518, 809, 1891) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4637) [M::mem_pestat] mean and std.dev: (1084.44, 1100.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6010) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 907.589 CPU sec, 52.681 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (69, 666869, 26, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (45, 81, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675) [M::mem_pestat] mean and std.dev: (95.79, 91.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 885) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.55, 75.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 123, 452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1282) [M::mem_pestat] mean and std.dev: (140.50, 170.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1697) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (172, 922, 1186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3214) [M::mem_pestat] mean and std.dev: (799.67, 616.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4228) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 905.663 CPU sec, 40.245 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (73, 666602, 38, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (42, 67, 155) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 381) [M::mem_pestat] mean and std.dev: (79.63, 57.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 494) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.53, 75.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 81, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 446) [M::mem_pestat] mean and std.dev: (60.50, 48.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 587) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (589, 1023, 2004) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4834) [M::mem_pestat] mean and std.dev: (1194.33, 1000.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6249) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1231018 sequences (184652700 bp)... [M::mem_process_seqs] Processed 1600000 reads in 906.819 CPU sec, 53.251 real sec [M::process] 0 single-end sequences; 1231018 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (60, 513015, 30, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (45, 77, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 396) [M::mem_pestat] mean and std.dev: (89.11, 63.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 513) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (215, 263, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 524) [M::mem_pestat] mean and std.dev: (269.37, 75.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 123, 500) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1446) [M::mem_pestat] mean and std.dev: (155.71, 258.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1919) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1231018 reads in 699.639 CPU sec, 30.814 real sec [main] Version: 0.7.17-r1188 [main] CMD: /home/csyk2proj/tools/bwa.kit/bwa mem -p -t24 -R@RG\tID:KPGP-00351\tPL:ILLUMINA\tSM:KPGP-00351 -C /home/csyk2proj/data/bwa.kit/hs38DH.fa - [main] Real time: 62109.487 sec; CPU: 704267.949 sec