[M::bwa_idx_load_from_disk] read 3171 ALT contigs [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 666523, 69, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (377, 574, 1282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3092) [M::mem_pestat] mean and std.dev: (607.50, 384.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3997) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.86, 79.54) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 26, 56) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 144) [M::mem_pestat] mean and std.dev: (25.05, 23.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 188) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1031.890 CPU sec, 60.056 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 666839, 62, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (278, 341, 964) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2336) [M::mem_pestat] mean and std.dev: (518.47, 362.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3022) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.66, 79.44) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 35, 61) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 141) [M::mem_pestat] mean and std.dev: (34.90, 23.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 181) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1296, 1854, 2835) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5913) [M::mem_pestat] mean and std.dev: (1791.43, 1127.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7452) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1021.414 CPU sec, 45.214 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 667077, 81, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (281, 432, 1093) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2717) [M::mem_pestat] mean and std.dev: (555.47, 381.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3529) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.73, 79.50) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 28, 56) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 142) [M::mem_pestat] mean and std.dev: (27.20, 24.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 185) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1028.965 CPU sec, 59.474 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666183, 65, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (453, 879, 1747) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4335) [M::mem_pestat] mean and std.dev: (1002.55, 658.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5629) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.62, 79.48) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 24, 92) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 250) [M::mem_pestat] mean and std.dev: (32.91, 32.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 329) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (346, 555, 2075) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5533) [M::mem_pestat] mean and std.dev: (822.36, 704.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7262) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1028.626 CPU sec, 45.784 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 666810, 67, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (290, 471, 1062) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2606) [M::mem_pestat] mean and std.dev: (693.06, 614.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3378) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.76, 79.68) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 44, 371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1073) [M::mem_pestat] mean and std.dev: (73.43, 141.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1424) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1021.326 CPU sec, 58.704 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 667341, 61, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (281, 512, 1078) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2672) [M::mem_pestat] mean and std.dev: (589.87, 418.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3469) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.76, 79.45) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 35, 69) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 173) [M::mem_pestat] mean and std.dev: (32.08, 25.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 225) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1015.703 CPU sec, 45.075 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666476, 60, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (268, 899, 1642) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4390) [M::mem_pestat] mean and std.dev: (819.20, 683.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5764) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.68, 79.44) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 49, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (53.13, 55.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 638) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1109, 1953, 3515) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8327) [M::mem_pestat] mean and std.dev: (2226.36, 1502.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10733) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1026.365 CPU sec, 58.823 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 666070, 68, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (188, 455, 542) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1250) [M::mem_pestat] mean and std.dev: (346.22, 164.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1604) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.82, 79.53) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 34, 134) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 374) [M::mem_pestat] mean and std.dev: (42.84, 61.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 494) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1024.614 CPU sec, 45.466 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666464, 74, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (412, 452, 1303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3085) [M::mem_pestat] mean and std.dev: (685.90, 427.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3976) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.70, 79.48) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 40, 136) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 368) [M::mem_pestat] mean and std.dev: (44.64, 48.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 484) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (775, 1188, 1647) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3391) [M::mem_pestat] mean and std.dev: (946.00, 499.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4263) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1026.636 CPU sec, 58.974 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 667083, 60, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (387, 952, 1349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3273) [M::mem_pestat] mean and std.dev: (823.13, 570.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4235) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.71, 79.64) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 45, 106) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 286) [M::mem_pestat] mean and std.dev: (44.96, 42.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 376) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1158, 1383, 2928) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6468) [M::mem_pestat] mean and std.dev: (2171.60, 1873.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9666) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1013.236 CPU sec, 45.235 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 666434, 55, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (353, 1022, 1501) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3797) [M::mem_pestat] mean and std.dev: (1043.23, 855.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4945) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.78, 79.46) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 30, 65) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 165) [M::mem_pestat] mean and std.dev: (32.15, 30.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 215) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1025.797 CPU sec, 58.889 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 667450, 66, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (530, 1104, 1483) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3389) [M::mem_pestat] mean and std.dev: (1218.50, 855.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4641) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.78, 79.52) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 33, 70) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 174) [M::mem_pestat] mean and std.dev: (31.68, 27.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 226) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (561, 975, 1515) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3423) [M::mem_pestat] mean and std.dev: (970.93, 565.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4377) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1017.773 CPU sec, 45.224 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 666147, 56, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (466, 861, 2069) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5275) [M::mem_pestat] mean and std.dev: (1082.69, 757.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6878) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.70, 79.33) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (11, 35, 96) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 266) [M::mem_pestat] mean and std.dev: (40.70, 44.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 351) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1026.075 CPU sec, 59.096 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 666786, 53, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (356, 435, 884) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1940) [M::mem_pestat] mean and std.dev: (531.38, 426.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2468) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.87, 79.57) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 37, 97) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 261) [M::mem_pestat] mean and std.dev: (40.86, 43.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 343) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (908, 1374, 1770) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3494) [M::mem_pestat] mean and std.dev: (1236.67, 410.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4356) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1016.596 CPU sec, 45.223 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 666239, 59, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (225, 308, 1506) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4068) [M::mem_pestat] mean and std.dev: (733.08, 806.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5349) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.73, 79.46) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 33, 71) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 181) [M::mem_pestat] mean and std.dev: (35.29, 32.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 236) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1021.977 CPU sec, 59.116 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666563, 49, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (228, 280, 563) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1233) [M::mem_pestat] mean and std.dev: (366.30, 267.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1568) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.84, 79.40) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 29, 56) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 140) [M::mem_pestat] mean and std.dev: (29.71, 23.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 182) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1019.376 CPU sec, 45.588 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 666986, 56, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (639, 1258, 1456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3090) [M::mem_pestat] mean and std.dev: (991.64, 585.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3907) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.70, 79.42) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 36, 99) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 273) [M::mem_pestat] mean and std.dev: (37.02, 35.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 360) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1200, 1534, 1992) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3576) [M::mem_pestat] mean and std.dev: (1654.75, 746.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4640) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1024.014 CPU sec, 58.984 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 666321, 66, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (380, 1616, 2306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6158) [M::mem_pestat] mean and std.dev: (1387.13, 1069.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8084) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.82, 79.49) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 29, 66) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 172) [M::mem_pestat] mean and std.dev: (29.81, 27.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 225) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (774, 1233, 3381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8595) [M::mem_pestat] mean and std.dev: (2191.92, 2225.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11202) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1017.430 CPU sec, 45.391 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 666669, 60, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (260, 523, 1154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2942) [M::mem_pestat] mean and std.dev: (711.71, 522.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3836) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.65, 79.68) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 34, 97) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 259) [M::mem_pestat] mean and std.dev: (36.98, 37.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 340) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (452, 885, 1276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2924) [M::mem_pestat] mean and std.dev: (899.50, 628.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3748) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1021.403 CPU sec, 58.065 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 666836, 60, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (314, 1194, 1365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3467) [M::mem_pestat] mean and std.dev: (1103.80, 896.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4690) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.65, 79.47) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 43, 463) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1357) [M::mem_pestat] mean and std.dev: (81.98, 175.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1804) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1010.562 CPU sec, 44.879 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 666634, 64, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (293, 569, 956) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2282) [M::mem_pestat] mean and std.dev: (730.42, 556.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2956) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.56, 79.56) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 38, 379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1093) [M::mem_pestat] mean and std.dev: (54.12, 80.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1450) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (857, 1785, 3116) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7634) [M::mem_pestat] mean and std.dev: (2352.82, 2250.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11356) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1014.176 CPU sec, 49.522 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 666860, 79, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (770, 1201, 1567) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3161) [M::mem_pestat] mean and std.dev: (1129.77, 721.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4015) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.81, 79.58) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 36, 115) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 315) [M::mem_pestat] mean and std.dev: (41.09, 39.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 415) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (855, 1666, 1918) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4044) [M::mem_pestat] mean and std.dev: (1357.23, 697.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5107) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1015.353 CPU sec, 57.380 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 666366, 58, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (285, 1133, 1465) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3825) [M::mem_pestat] mean and std.dev: (1024.57, 851.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5005) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.70, 79.59) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 29, 66) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 174) [M::mem_pestat] mean and std.dev: (31.25, 28.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 228) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (623, 1042, 1731) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3947) [M::mem_pestat] mean and std.dev: (1060.67, 699.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5055) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1017.277 CPU sec, 45.483 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 666258, 80, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (346, 529, 1708) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4432) [M::mem_pestat] mean and std.dev: (1009.13, 815.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5794) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.84, 79.38) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 30, 70) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 184) [M::mem_pestat] mean and std.dev: (32.17, 31.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 241) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1021.671 CPU sec, 58.647 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 666709, 52, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (217, 296, 781) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1909) [M::mem_pestat] mean and std.dev: (301.45, 176.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2473) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.69, 79.51) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 30, 109) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 295) [M::mem_pestat] mean and std.dev: (38.09, 47.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 388) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (369, 847, 1493) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3741) [M::mem_pestat] mean and std.dev: (820.83, 595.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4865) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1015.216 CPU sec, 45.316 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 666533, 56, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (171, 465, 733) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1857) [M::mem_pestat] mean and std.dev: (504.33, 393.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2419) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.73, 79.53) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 37, 139) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 389) [M::mem_pestat] mean and std.dev: (43.98, 56.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 514) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (760, 982, 1737) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3691) [M::mem_pestat] mean and std.dev: (857.25, 445.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4668) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1026.041 CPU sec, 59.563 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 666335, 58, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (226, 337, 911) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2281) [M::mem_pestat] mean and std.dev: (496.89, 431.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2966) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.76, 79.46) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 34, 90) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 244) [M::mem_pestat] mean and std.dev: (35.33, 34.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 321) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1700, 3438, 3635) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7505) [M::mem_pestat] mean and std.dev: (2716.85, 1580.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9440) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1011.365 CPU sec, 44.856 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 667072, 61, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (386, 1467, 1640) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4148) [M::mem_pestat] mean and std.dev: (1203.92, 833.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5402) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.83, 79.45) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 34, 83) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 219) [M::mem_pestat] mean and std.dev: (31.83, 22.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 287) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1127, 1270, 1629) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 2633) [M::mem_pestat] mean and std.dev: (1268.80, 510.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3312) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1015.893 CPU sec, 58.666 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 667080, 69, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (231, 846, 1812) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4974) [M::mem_pestat] mean and std.dev: (827.11, 803.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6555) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.74, 79.65) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 36, 124) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 336) [M::mem_pestat] mean and std.dev: (44.40, 43.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 442) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (477, 1265, 1769) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4353) [M::mem_pestat] mean and std.dev: (1136.31, 696.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5645) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1010.081 CPU sec, 45.029 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 666457, 56, 10) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.69, 79.69) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 37, 78) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 202) [M::mem_pestat] mean and std.dev: (43.46, 38.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 264) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (635, 1178, 1475) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3155) [M::mem_pestat] mean and std.dev: (1085.50, 436.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3995) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1024.759 CPU sec, 59.374 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 666335, 68, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (223, 444, 1080) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2794) [M::mem_pestat] mean and std.dev: (782.90, 758.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3818) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.82, 79.58) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (11, 38, 117) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 329) [M::mem_pestat] mean and std.dev: (42.75, 48.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 435) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (862, 1086, 1649) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3223) [M::mem_pestat] mean and std.dev: (1112.25, 577.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4010) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1012.818 CPU sec, 45.212 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 666728, 69, 12) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.79, 79.53) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 46, 87) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 225) [M::mem_pestat] mean and std.dev: (44.73, 39.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 294) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (388, 1221, 2051) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5377) [M::mem_pestat] mean and std.dev: (1292.17, 1016.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7040) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1022.659 CPU sec, 58.669 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 667185, 71, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (739, 943, 1715) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3667) [M::mem_pestat] mean and std.dev: (1089.75, 805.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4643) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.76, 79.49) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 46, 120) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 324) [M::mem_pestat] mean and std.dev: (46.03, 49.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 426) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1009.297 CPU sec, 44.716 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 666517, 70, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (285, 459, 2295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6315) [M::mem_pestat] mean and std.dev: (935.67, 946.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8325) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.69, 79.49) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 42, 401) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1171) [M::mem_pestat] mean and std.dev: (102.38, 207.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1556) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (853, 1323, 2951) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7147) [M::mem_pestat] mean and std.dev: (1840.31, 1777.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9245) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1017.810 CPU sec, 58.315 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666597, 68, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (503, 687, 1652) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3950) [M::mem_pestat] mean and std.dev: (1124.09, 968.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5099) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.82, 79.53) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 32, 121) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 337) [M::mem_pestat] mean and std.dev: (36.22, 40.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 445) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1010.561 CPU sec, 45.129 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 666520, 63, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (291, 800, 1284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3270) [M::mem_pestat] mean and std.dev: (726.53, 475.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4263) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.74, 79.47) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 34, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 527) [M::mem_pestat] mean and std.dev: (55.16, 81.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 696) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (806, 1571, 2358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5462) [M::mem_pestat] mean and std.dev: (1500.00, 905.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7014) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1019.553 CPU sec, 59.216 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 667419, 64, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (456, 924, 1474) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3510) [M::mem_pestat] mean and std.dev: (980.29, 884.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4528) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.87, 79.51) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 31, 59) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 151) [M::mem_pestat] mean and std.dev: (29.77, 25.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 197) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1003.898 CPU sec, 44.704 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666669, 64, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (310, 418, 2715) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7525) [M::mem_pestat] mean and std.dev: (1019.60, 1249.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9930) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.77, 79.39) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 29, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628) [M::mem_pestat] mean and std.dev: (71.95, 121.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 833) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1035, 1203, 1789) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3297) [M::mem_pestat] mean and std.dev: (1287.45, 521.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4051) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1022.193 CPU sec, 60.320 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 666889, 70, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (494, 917, 1395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3197) [M::mem_pestat] mean and std.dev: (915.75, 624.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4098) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.50, 79.50) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 44, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1008) [M::mem_pestat] mean and std.dev: (70.45, 150.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1336) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (410, 889, 1215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2825) [M::mem_pestat] mean and std.dev: (828.93, 591.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3630) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1010.470 CPU sec, 44.863 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 666298, 59, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (312, 1232, 1558) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4050) [M::mem_pestat] mean and std.dev: (834.50, 546.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5296) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.70, 79.51) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 29, 76) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 202) [M::mem_pestat] mean and std.dev: (40.24, 40.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 265) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (416, 947, 2202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5774) [M::mem_pestat] mean and std.dev: (1107.50, 874.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7560) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1022.011 CPU sec, 58.988 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 666702, 60, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.68, 79.49) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 32, 49) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 123) [M::mem_pestat] mean and std.dev: (25.45, 17.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 160) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1006.881 CPU sec, 44.906 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 666252, 81, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (365, 862, 1250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3020) [M::mem_pestat] mean and std.dev: (785.19, 449.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3905) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.69, 79.52) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 36, 80) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 214) [M::mem_pestat] mean and std.dev: (35.49, 34.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 281) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1014.176 CPU sec, 52.819 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 666412, 43, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (556, 1070, 1415) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3133) [M::mem_pestat] mean and std.dev: (1063.25, 659.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3992) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.69, 79.42) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 29, 78) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 202) [M::mem_pestat] mean and std.dev: (32.08, 28.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 264) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1018.517 CPU sec, 59.124 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 666649, 41, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (504, 1224, 1304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2904) [M::mem_pestat] mean and std.dev: (1000.10, 574.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3704) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.78, 79.50) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 40, 73) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 167) [M::mem_pestat] mean and std.dev: (34.81, 18.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 214) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (507, 1150, 1489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3453) [M::mem_pestat] mean and std.dev: (1025.21, 597.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4435) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1009.601 CPU sec, 44.912 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 666652, 58, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (343, 821, 1669) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4321) [M::mem_pestat] mean and std.dev: (926.47, 816.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5647) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.62, 79.48) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 30, 55) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 135) [M::mem_pestat] mean and std.dev: (28.65, 19.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 175) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1016.960 CPU sec, 58.353 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666808, 73, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (362, 855, 1763) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4565) [M::mem_pestat] mean and std.dev: (1070.00, 827.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5966) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.80, 79.65) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 31, 86) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 230) [M::mem_pestat] mean and std.dev: (26.44, 20.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 302) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (364, 799, 1541) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3895) [M::mem_pestat] mean and std.dev: (825.70, 516.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5072) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1010.812 CPU sec, 44.958 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 666521, 72, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (261, 388, 1341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3501) [M::mem_pestat] mean and std.dev: (752.33, 763.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4581) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.76, 79.53) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 34, 76) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 204) [M::mem_pestat] mean and std.dev: (36.73, 39.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 268) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1010, 1442, 2963) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6869) [M::mem_pestat] mean and std.dev: (1849.25, 1632.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8822) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1013.170 CPU sec, 57.505 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 667170, 58, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (441, 811, 1118) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2472) [M::mem_pestat] mean and std.dev: (786.61, 451.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3149) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.72, 79.41) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (9, 22, 92) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 258) [M::mem_pestat] mean and std.dev: (27.39, 30.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 341) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1008.288 CPU sec, 44.994 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 666409, 62, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (265, 821, 1023) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2539) [M::mem_pestat] mean and std.dev: (651.82, 393.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3297) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.76, 79.54) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 31, 87) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 231) [M::mem_pestat] mean and std.dev: (33.41, 30.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 303) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (356, 1282, 2170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5798) [M::mem_pestat] mean and std.dev: (1234.60, 849.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7612) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1019.866 CPU sec, 58.469 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 666376, 49, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (219, 411, 1401) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3765) [M::mem_pestat] mean and std.dev: (587.67, 513.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4947) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.64, 79.57) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 36, 56) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 138) [M::mem_pestat] mean and std.dev: (31.95, 24.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 179) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1011.226 CPU sec, 45.052 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 666586, 52, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (569, 1124, 3065) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8057) [M::mem_pestat] mean and std.dev: (1553.80, 1232.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10553) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.78, 79.60) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 39, 140) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 376) [M::mem_pestat] mean and std.dev: (43.27, 52.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 494) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (910, 955, 1222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (286, 1846) [M::mem_pestat] mean and std.dev: (1007.18, 135.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2158) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1018.236 CPU sec, 58.071 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 666645, 71, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (352, 1319, 1989) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5263) [M::mem_pestat] mean and std.dev: (1238.31, 915.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6900) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.82, 79.46) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 36, 109) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 301) [M::mem_pestat] mean and std.dev: (51.03, 63.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 397) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (740, 1118, 1704) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3632) [M::mem_pestat] mean and std.dev: (1153.78, 555.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4596) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1008.963 CPU sec, 44.886 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 666690, 64, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (296, 1321, 1705) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4523) [M::mem_pestat] mean and std.dev: (1212.69, 1065.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5932) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.83, 79.31) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 37, 145) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (42.45, 58.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 529) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1351, 1439, 2415) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4543) [M::mem_pestat] mean and std.dev: (1563.58, 762.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5607) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1016.414 CPU sec, 57.753 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 666772, 49, 16) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.69, 79.61) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 31, 57) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 135) [M::mem_pestat] mean and std.dev: (29.36, 20.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 174) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1028, 1197, 1861) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3527) [M::mem_pestat] mean and std.dev: (1199.79, 614.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4360) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1009.870 CPU sec, 45.066 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 666591, 65, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (294, 974, 2104) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5724) [M::mem_pestat] mean and std.dev: (1014.55, 862.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7534) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.80, 79.53) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 28, 73) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 189) [M::mem_pestat] mean and std.dev: (36.73, 38.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 247) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1154, 1348, 2333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4691) [M::mem_pestat] mean and std.dev: (1599.18, 862.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5870) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1016.148 CPU sec, 59.120 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 667078, 51, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (478, 1242, 1732) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4240) [M::mem_pestat] mean and std.dev: (1143.67, 968.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5494) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.95, 79.51) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 31, 143) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 397) [M::mem_pestat] mean and std.dev: (36.85, 41.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 524) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1006.397 CPU sec, 44.822 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 667271, 55, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (293, 734, 3169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8921) [M::mem_pestat] mean and std.dev: (1801.00, 1928.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11797) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.56, 79.65) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 38, 1121) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3339) [M::mem_pestat] mean and std.dev: (400.59, 800.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4448) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1021.405 CPU sec, 59.892 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 666243, 58, 15) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.81, 79.49) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 29, 127) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 355) [M::mem_pestat] mean and std.dev: (39.19, 51.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 469) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (832, 1423, 2125) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4711) [M::mem_pestat] mean and std.dev: (1587.93, 941.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6004) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1004.944 CPU sec, 44.742 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 666620, 71, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (217, 518, 1146) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3004) [M::mem_pestat] mean and std.dev: (627.89, 475.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3933) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.77, 79.64) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 32, 69) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 167) [M::mem_pestat] mean and std.dev: (31.78, 24.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 216) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (372, 873, 1635) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4161) [M::mem_pestat] mean and std.dev: (995.00, 801.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5424) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1009.294 CPU sec, 58.554 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 666226, 73, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (296, 501, 1122) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2774) [M::mem_pestat] mean and std.dev: (655.44, 458.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3600) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.75, 79.45) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 33, 116) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 322) [M::mem_pestat] mean and std.dev: (45.28, 64.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 425) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1011.590 CPU sec, 45.168 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 666723, 66, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (322, 528, 1180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2896) [M::mem_pestat] mean and std.dev: (582.18, 453.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3754) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.76, 79.44) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 32, 48) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 116) [M::mem_pestat] mean and std.dev: (26.89, 18.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 150) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (260, 1120, 2185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6035) [M::mem_pestat] mean and std.dev: (1002.22, 833.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7960) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1013.433 CPU sec, 58.300 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 667029, 68, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (324, 571, 1479) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3789) [M::mem_pestat] mean and std.dev: (933.80, 761.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4944) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.71, 79.63) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 42, 106) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (37.39, 30.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 367) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1006.934 CPU sec, 44.985 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 666415, 79, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (601, 1072, 1365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2893) [M::mem_pestat] mean and std.dev: (944.20, 583.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3657) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.84, 79.61) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 37, 144) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (54.51, 70.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 540) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1450, 1648, 1809) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (732, 2527) [M::mem_pestat] mean and std.dev: (1582.90, 261.77) [M::mem_pestat] low and high boundaries for proper pairs: (373, 2886) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1016.064 CPU sec, 56.555 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 666374, 74, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (188, 1239, 1284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3476) [M::mem_pestat] mean and std.dev: (1128.80, 986.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5077) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.83, 79.57) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 47, 116) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 308) [M::mem_pestat] mean and std.dev: (51.32, 56.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 404) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1013.358 CPU sec, 58.125 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 666572, 59, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (339, 999, 1538) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3936) [M::mem_pestat] mean and std.dev: (818.33, 663.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5135) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.88, 79.62) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 28, 51) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 125) [M::mem_pestat] mean and std.dev: (28.62, 25.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 162) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (221, 821, 1350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3608) [M::mem_pestat] mean and std.dev: (767.92, 470.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4737) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1008.002 CPU sec, 45.125 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 666394, 67, 17) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.75, 79.60) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 39, 72) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 184) [M::mem_pestat] mean and std.dev: (38.58, 33.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 240) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (881, 1028, 2765) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6533) [M::mem_pestat] mean and std.dev: (1511.06, 1100.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8417) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1015.062 CPU sec, 57.812 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 666916, 53, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (259, 367, 829) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1969) [M::mem_pestat] mean and std.dev: (544.22, 458.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2539) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.66, 79.44) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 37, 104) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 264) [M::mem_pestat] mean and std.dev: (36.38, 26.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 344) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (997, 1144, 1665) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3001) [M::mem_pestat] mean and std.dev: (1104.10, 731.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4031) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1008.831 CPU sec, 45.071 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 666448, 71, 10) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.65, 79.36) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 32, 111) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 301) [M::mem_pestat] mean and std.dev: (41.85, 50.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 396) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (899, 1098, 1155) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (387, 1667) [M::mem_pestat] mean and std.dev: (983.00, 231.83) [M::mem_pestat] low and high boundaries for proper pairs: (56, 1923) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1015.936 CPU sec, 60.151 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 666765, 71, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (280, 591, 3940) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11260) [M::mem_pestat] mean and std.dev: (1789.42, 2348.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14920) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.79, 79.61) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 41, 117) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 317) [M::mem_pestat] mean and std.dev: (43.14, 45.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 417) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (808, 1016, 1689) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3451) [M::mem_pestat] mean and std.dev: (1166.15, 771.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4332) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1004.581 CPU sec, 45.002 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666738, 80, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (334, 461, 943) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2161) [M::mem_pestat] mean and std.dev: (685.91, 528.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2801) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.82, 79.56) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 33, 79) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 207) [M::mem_pestat] mean and std.dev: (37.43, 37.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 271) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (476, 1060, 1702) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4154) [M::mem_pestat] mean and std.dev: (1213.92, 930.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5380) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1016.612 CPU sec, 59.370 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 666517, 61, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (250, 1084, 1505) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4015) [M::mem_pestat] mean and std.dev: (1098.21, 1022.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5270) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.62, 79.40) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 35, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 535) [M::mem_pestat] mean and std.dev: (62.82, 93.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 709) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1011.262 CPU sec, 44.988 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 666394, 72, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (268, 469, 1702) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4570) [M::mem_pestat] mean and std.dev: (679.67, 626.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6004) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.73, 79.34) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 39, 116) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 312) [M::mem_pestat] mean and std.dev: (51.75, 63.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 410) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1012.183 CPU sec, 58.153 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 666694, 69, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (211, 1276, 1775) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4903) [M::mem_pestat] mean and std.dev: (1253.11, 1032.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6467) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.62, 79.42) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 34, 75) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 181) [M::mem_pestat] mean and std.dev: (32.22, 23.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 234) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (728, 1417, 1946) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4382) [M::mem_pestat] mean and std.dev: (1294.33, 801.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5600) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1006.496 CPU sec, 44.744 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 666893, 77, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (372, 815, 1650) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4206) [M::mem_pestat] mean and std.dev: (882.06, 799.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5484) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.84, 79.86) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 31, 69) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 177) [M::mem_pestat] mean and std.dev: (31.95, 30.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 231) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 991.546 CPU sec, 47.551 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 666651, 59, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (741, 992, 1797) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3909) [M::mem_pestat] mean and std.dev: (1157.54, 907.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4965) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.82, 79.50) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 25, 76) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 202) [M::mem_pestat] mean and std.dev: (35.49, 38.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 265) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (984, 1294, 3088) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7296) [M::mem_pestat] mean and std.dev: (1696.58, 950.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9400) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1019.800 CPU sec, 58.643 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 667092, 61, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (363, 623, 1038) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2388) [M::mem_pestat] mean and std.dev: (587.21, 404.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3063) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.71, 79.38) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 39, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645) [M::mem_pestat] mean and std.dev: (66.65, 105.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 853) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1005.765 CPU sec, 44.695 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 666474, 67, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (980, 1185, 1775) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3365) [M::mem_pestat] mean and std.dev: (1160.36, 594.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4160) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.83, 79.54) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 37, 108) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 294) [M::mem_pestat] mean and std.dev: (39.44, 37.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 387) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1009.228 CPU sec, 57.848 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 666401, 65, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (676, 1207, 4419) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11905) [M::mem_pestat] mean and std.dev: (2204.38, 2365.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15648) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.74, 79.75) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 30, 68) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 174) [M::mem_pestat] mean and std.dev: (32.47, 29.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 227) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1007.568 CPU sec, 45.025 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 666556, 63, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (295, 402, 957) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2281) [M::mem_pestat] mean and std.dev: (438.00, 299.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2943) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.90, 79.41) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 34, 135) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 363) [M::mem_pestat] mean and std.dev: (39.56, 41.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 477) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1010.630 CPU sec, 58.689 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 666694, 51, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (250, 443, 822) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1966) [M::mem_pestat] mean and std.dev: (523.15, 463.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2538) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.89, 79.63) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 50, 801) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2345) [M::mem_pestat] mean and std.dev: (209.49, 453.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3117) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1000, 1526, 1740) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3220) [M::mem_pestat] mean and std.dev: (1229.50, 597.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3960) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1004.548 CPU sec, 44.808 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 666041, 62, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (352, 642, 1595) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4081) [M::mem_pestat] mean and std.dev: (773.75, 564.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5324) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.69, 79.51) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 28, 96) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 264) [M::mem_pestat] mean and std.dev: (31.48, 34.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 348) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (864, 1555, 2050) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4422) [M::mem_pestat] mean and std.dev: (1447.54, 833.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5608) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1015.590 CPU sec, 58.830 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 666615, 53, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (352, 789, 958) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2170) [M::mem_pestat] mean and std.dev: (674.46, 392.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2776) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.74, 79.53) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 37, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800) [M::mem_pestat] mean and std.dev: (65.16, 99.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1062) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (986, 1150, 1382) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (194, 2174) [M::mem_pestat] mean and std.dev: (1164.71, 281.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2570) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1007.543 CPU sec, 44.926 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666668, 74, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (385, 435, 724) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1402) [M::mem_pestat] mean and std.dev: (533.40, 303.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1747) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.69, 79.46) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 29, 76) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 198) [M::mem_pestat] mean and std.dev: (30.32, 30.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 259) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1242, 1406, 2389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4683) [M::mem_pestat] mean and std.dev: (1616.47, 850.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5830) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1020.218 CPU sec, 59.866 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 667069, 53, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (281, 991, 1560) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4118) [M::mem_pestat] mean and std.dev: (847.40, 650.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5397) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.69, 79.50) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (9, 27, 112) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 318) [M::mem_pestat] mean and std.dev: (44.45, 59.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 421) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1005.024 CPU sec, 44.829 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 666951, 67, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (270, 536, 884) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2112) [M::mem_pestat] mean and std.dev: (563.62, 371.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2726) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.82, 79.51) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 27, 47) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 107) [M::mem_pestat] mean and std.dev: (28.33, 18.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 137) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1004.678 CPU sec, 56.929 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 666577, 79, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.72, 79.52) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 40, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606) [M::mem_pestat] mean and std.dev: (55.98, 96.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 803) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1001.709 CPU sec, 44.585 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 667450, 59, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.78, 79.67) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 33, 100) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 258) [M::mem_pestat] mean and std.dev: (35.44, 31.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1008.750 CPU sec, 57.710 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 666956, 76, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (292, 509, 2075) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5641) [M::mem_pestat] mean and std.dev: (987.17, 845.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7424) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.81, 79.55) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 37, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460) [M::mem_pestat] mean and std.dev: (44.29, 51.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 608) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 999.009 CPU sec, 44.618 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 666782, 48, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (299, 828, 1371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3515) [M::mem_pestat] mean and std.dev: (865.50, 684.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4587) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.83, 79.63) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (11, 26, 44) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 110) [M::mem_pestat] mean and std.dev: (24.88, 21.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 143) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1014.719 CPU sec, 58.822 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 665954, 69, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (388, 726, 1305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3139) [M::mem_pestat] mean and std.dev: (791.88, 603.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4056) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.88, 79.50) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 38, 129) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 353) [M::mem_pestat] mean and std.dev: (39.91, 48.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 465) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (551, 1378, 1814) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4340) [M::mem_pestat] mean and std.dev: (1268.92, 934.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5603) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1001.364 CPU sec, 44.833 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 666447, 61, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (480, 696, 1270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2850) [M::mem_pestat] mean and std.dev: (910.31, 627.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3640) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.80, 79.65) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 35, 135) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 363) [M::mem_pestat] mean and std.dev: (34.98, 29.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 477) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (294, 983, 2266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6210) [M::mem_pestat] mean and std.dev: (1375.29, 1181.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8182) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1015.749 CPU sec, 58.810 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 666762, 57, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (295, 718, 1277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3241) [M::mem_pestat] mean and std.dev: (800.08, 632.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4223) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.70, 79.47) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 25, 59) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 153) [M::mem_pestat] mean and std.dev: (27.71, 24.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 200) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1000.259 CPU sec, 44.319 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 667197, 61, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (308, 464, 1523) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3953) [M::mem_pestat] mean and std.dev: (974.05, 962.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5168) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.77, 79.43) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 34, 79) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 203) [M::mem_pestat] mean and std.dev: (39.15, 36.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 265) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (882, 1219, 1910) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3966) [M::mem_pestat] mean and std.dev: (1070.93, 582.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4994) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1014.261 CPU sec, 58.453 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 667238, 64, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (316, 487, 979) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2305) [M::mem_pestat] mean and std.dev: (762.42, 624.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3260) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 383, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.97, 79.46) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 36, 67) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 165) [M::mem_pestat] mean and std.dev: (34.46, 27.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 214) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (838, 1107, 2211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4957) [M::mem_pestat] mean and std.dev: (1425.45, 1089.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6330) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1002.277 CPU sec, 44.920 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 666364, 57, 12) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.80, 79.49) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 37, 80) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 204) [M::mem_pestat] mean and std.dev: (43.35, 41.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 266) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (751, 1490, 2729) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6685) [M::mem_pestat] mean and std.dev: (1606.58, 1060.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8663) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1002.345 CPU sec, 51.001 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 666770, 55, 10) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.82, 79.50) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 30, 71) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 181) [M::mem_pestat] mean and std.dev: (37.81, 34.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 236) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (960, 1284, 2173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4599) [M::mem_pestat] mean and std.dev: (1335.11, 520.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5812) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1012.734 CPU sec, 58.475 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 666663, 74, 11) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.71, 79.49) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 36, 98) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 268) [M::mem_pestat] mean and std.dev: (36.72, 36.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 353) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1040, 1189, 2033) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4019) [M::mem_pestat] mean and std.dev: (1653.36, 890.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5216) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1005.017 CPU sec, 44.831 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 667164, 52, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (274, 951, 1170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2962) [M::mem_pestat] mean and std.dev: (764.18, 581.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3858) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.67, 79.38) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 54, 990) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2918) [M::mem_pestat] mean and std.dev: (256.39, 570.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3882) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1011.181 CPU sec, 59.130 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 666701, 68, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (315, 540, 1821) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4833) [M::mem_pestat] mean and std.dev: (930.47, 685.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6339) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.57, 79.51) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 34, 88) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 234) [M::mem_pestat] mean and std.dev: (40.53, 41.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 307) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (905, 1480, 3190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7760) [M::mem_pestat] mean and std.dev: (1616.25, 1162.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10045) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1012.315 CPU sec, 45.077 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 666503, 77, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (316, 1112, 1738) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4582) [M::mem_pestat] mean and std.dev: (1094.07, 938.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6004) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.79, 79.56) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 35, 74) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 192) [M::mem_pestat] mean and std.dev: (32.39, 25.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 251) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1010.706 CPU sec, 59.433 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 667234, 73, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (290, 1011, 1701) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4523) [M::mem_pestat] mean and std.dev: (934.82, 752.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5934) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.65, 79.55) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 40, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 511) [M::mem_pestat] mean and std.dev: (53.32, 76.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 677) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1004.453 CPU sec, 44.788 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 666391, 64, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (502, 1233, 2697) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7087) [M::mem_pestat] mean and std.dev: (1449.40, 1185.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9282) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.65, 79.61) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 34, 679) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1997) [M::mem_pestat] mean and std.dev: (188.08, 442.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2656) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1002.709 CPU sec, 57.862 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 667010, 73, 11) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.76, 79.59) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 39, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (45.98, 72.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 657) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (720, 1033, 1439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2877) [M::mem_pestat] mean and std.dev: (1109.82, 548.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3596) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 994.202 CPU sec, 44.125 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 666293, 63, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (336, 853, 1002) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2334) [M::mem_pestat] mean and std.dev: (710.17, 371.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3000) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.73, 79.66) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 32, 95) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 249) [M::mem_pestat] mean and std.dev: (39.98, 43.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 326) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 995.411 CPU sec, 56.920 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 667054, 66, 13) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.87, 79.45) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 28, 67) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 177) [M::mem_pestat] mean and std.dev: (27.83, 28.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 232) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (772, 1082, 2161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4939) [M::mem_pestat] mean and std.dev: (1278.08, 785.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6328) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 993.871 CPU sec, 44.268 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 666162, 66, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (512, 757, 1858) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4550) [M::mem_pestat] mean and std.dev: (1039.56, 1016.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5896) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.81, 79.56) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 35, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 859) [M::mem_pestat] mean and std.dev: (49.98, 88.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1140) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (511, 1009, 1394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3160) [M::mem_pestat] mean and std.dev: (889.56, 479.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4043) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1018.954 CPU sec, 60.203 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 667335, 67, 13) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.72, 79.55) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 34, 121) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 321) [M::mem_pestat] mean and std.dev: (49.09, 52.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 421) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1194, 1522, 5929) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15399) [M::mem_pestat] mean and std.dev: (2653.38, 2327.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20134) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 999.902 CPU sec, 44.278 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 666527, 64, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (302, 703, 1213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3035) [M::mem_pestat] mean and std.dev: (763.82, 617.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3946) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.62, 79.61) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 40, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703) [M::mem_pestat] mean and std.dev: (59.82, 91.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 930) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (646, 1019, 1269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2515) [M::mem_pestat] mean and std.dev: (895.00, 352.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3138) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1010.660 CPU sec, 59.306 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 667078, 62, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (384, 757, 1628) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4116) [M::mem_pestat] mean and std.dev: (977.00, 750.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5360) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.86, 79.45) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 34, 84) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 226) [M::mem_pestat] mean and std.dev: (39.43, 38.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 297) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (382, 1017, 1297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3127) [M::mem_pestat] mean and std.dev: (849.25, 522.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4042) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1001.751 CPU sec, 44.652 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 666677, 47, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (200, 519, 2160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6080) [M::mem_pestat] mean and std.dev: (1221.15, 1390.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8040) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.76, 79.46) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 33, 96) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 250) [M::mem_pestat] mean and std.dev: (35.26, 32.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 327) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1033, 1184, 1297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (505, 1825) [M::mem_pestat] mean and std.dev: (1175.00, 202.91) [M::mem_pestat] low and high boundaries for proper pairs: (241, 2089) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1013.284 CPU sec, 57.924 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 666414, 61, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (241, 478, 2185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6073) [M::mem_pestat] mean and std.dev: (832.17, 1071.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8017) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.67, 79.61) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 33, 67) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 177) [M::mem_pestat] mean and std.dev: (37.40, 35.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 232) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1348, 1737, 2324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4276) [M::mem_pestat] mean and std.dev: (1613.50, 660.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5252) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1011.894 CPU sec, 45.100 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 666557, 63, 14) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.72, 79.63) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 35, 70) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 176) [M::mem_pestat] mean and std.dev: (30.12, 21.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 229) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (699, 1267, 1391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2775) [M::mem_pestat] mean and std.dev: (1145.57, 577.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3467) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1012.481 CPU sec, 57.455 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 666414, 64, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (251, 304, 797) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1889) [M::mem_pestat] mean and std.dev: (347.20, 190.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2435) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.79, 79.61) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 33, 77) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 193) [M::mem_pestat] mean and std.dev: (35.08, 29.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 251) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (657, 999, 3392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8862) [M::mem_pestat] mean and std.dev: (1867.10, 1928.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11597) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1008.382 CPU sec, 44.938 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 666683, 59, 10) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.74, 79.53) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 40, 122) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 336) [M::mem_pestat] mean and std.dev: (47.61, 50.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 443) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1201, 1292, 1787) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (29, 2959) [M::mem_pestat] mean and std.dev: (1282.00, 217.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3545) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1006.162 CPU sec, 58.569 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 666934, 58, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (267, 657, 1075) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2691) [M::mem_pestat] mean and std.dev: (635.23, 391.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3499) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.88, 79.50) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 37, 79) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 205) [M::mem_pestat] mean and std.dev: (34.71, 27.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 268) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 993.242 CPU sec, 44.018 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 666853, 60, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (291, 562, 1410) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3648) [M::mem_pestat] mean and std.dev: (925.57, 991.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4891) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.68, 79.52) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 42, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (49.80, 63.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 601) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (647, 862, 2435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6011) [M::mem_pestat] mean and std.dev: (1095.50, 924.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7799) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1005.607 CPU sec, 56.133 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666969, 66, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (134, 244, 1857) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5303) [M::mem_pestat] mean and std.dev: (919.10, 1307.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7026) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.66, 79.52) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 46, 150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (42.41, 37.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 540) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1012.792 CPU sec, 59.642 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 666842, 69, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (295, 1090, 2150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5860) [M::mem_pestat] mean and std.dev: (1099.83, 849.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7715) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.72, 79.40) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 35, 99) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 263) [M::mem_pestat] mean and std.dev: (32.39, 24.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 345) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (277, 972, 1245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3181) [M::mem_pestat] mean and std.dev: (865.58, 574.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4149) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1002.351 CPU sec, 44.531 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 666473, 74, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (340, 637, 4471) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12733) [M::mem_pestat] mean and std.dev: (1686.00, 2039.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16864) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.57, 79.42) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 34, 80) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 204) [M::mem_pestat] mean and std.dev: (32.52, 27.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 266) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1157, 1223, 3488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8150) [M::mem_pestat] mean and std.dev: (1934.00, 1663.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10481) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1014.879 CPU sec, 59.370 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 667033, 66, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (373, 606, 1036) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2362) [M::mem_pestat] mean and std.dev: (850.93, 663.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3507) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.74, 79.74) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 39, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 436) [M::mem_pestat] mean and std.dev: (43.46, 45.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 574) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (695, 777, 2253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5369) [M::mem_pestat] mean and std.dev: (1537.50, 1350.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6941) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1003.041 CPU sec, 44.546 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666832, 59, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (484, 729, 906) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1750) [M::mem_pestat] mean and std.dev: (656.00, 268.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2172) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.91, 79.55) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 28, 75) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 193) [M::mem_pestat] mean and std.dev: (35.79, 30.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 252) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (396, 744, 1209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2835) [M::mem_pestat] mean and std.dev: (783.00, 464.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3648) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1019.139 CPU sec, 59.255 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 666043, 61, 17) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.87, 79.51) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (11, 25, 55) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 143) [M::mem_pestat] mean and std.dev: (28.47, 28.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 187) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (845, 1130, 2691) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6383) [M::mem_pestat] mean and std.dev: (1531.76, 974.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8229) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1003.290 CPU sec, 44.841 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 666697, 61, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (417, 1061, 1544) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3798) [M::mem_pestat] mean and std.dev: (1092.46, 852.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4925) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.68, 79.44) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (11, 44, 412) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1214) [M::mem_pestat] mean and std.dev: (127.92, 249.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1615) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1012, 1273, 3100) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7276) [M::mem_pestat] mean and std.dev: (1737.00, 1277.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9364) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1001.592 CPU sec, 58.115 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 667162, 71, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (445, 784, 1611) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3943) [M::mem_pestat] mean and std.dev: (874.27, 614.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5109) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.57, 79.65) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 36, 119) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 331) [M::mem_pestat] mean and std.dev: (33.83, 32.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 437) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1003.594 CPU sec, 44.599 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 666650, 71, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (606, 1261, 1852) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4344) [M::mem_pestat] mean and std.dev: (1310.53, 973.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5590) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.64, 79.60) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 40, 76) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 174) [M::mem_pestat] mean and std.dev: (41.50, 28.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 223) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (707, 914, 1113) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1925) [M::mem_pestat] mean and std.dev: (825.33, 278.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2331) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1005.705 CPU sec, 58.467 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666360, 73, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (259, 365, 1498) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3976) [M::mem_pestat] mean and std.dev: (692.90, 652.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5215) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.80, 79.51) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (11, 33, 126) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 356) [M::mem_pestat] mean and std.dev: (31.64, 32.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 471) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (765, 1122, 1804) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3882) [M::mem_pestat] mean and std.dev: (1218.00, 844.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4921) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 998.351 CPU sec, 44.463 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 666684, 74, 14) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.63, 79.58) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 53, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1400) [M::mem_pestat] mean and std.dev: (120.62, 247.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1859) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (585, 975, 1697) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3921) [M::mem_pestat] mean and std.dev: (960.25, 537.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5033) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 984.711 CPU sec, 45.797 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 666451, 58, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (297, 431, 1185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2961) [M::mem_pestat] mean and std.dev: (793.67, 609.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3849) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.63, 79.57) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 32, 131) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 359) [M::mem_pestat] mean and std.dev: (46.85, 63.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 473) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (478, 832, 1179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2581) [M::mem_pestat] mean and std.dev: (733.25, 388.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3282) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1004.703 CPU sec, 56.798 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 666370, 69, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (321, 668, 1088) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2622) [M::mem_pestat] mean and std.dev: (708.72, 564.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3389) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.71, 79.38) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 35, 61) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 153) [M::mem_pestat] mean and std.dev: (31.69, 27.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 199) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1003.332 CPU sec, 44.574 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666605, 64, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (157, 292, 731) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1879) [M::mem_pestat] mean and std.dev: (449.00, 477.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2453) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.73, 79.62) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 30, 71) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 187) [M::mem_pestat] mean and std.dev: (30.26, 29.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 245) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (819, 1304, 1974) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4284) [M::mem_pestat] mean and std.dev: (1528.94, 918.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5439) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1002.001 CPU sec, 56.766 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666995, 59, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (417, 896, 1188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2730) [M::mem_pestat] mean and std.dev: (923.27, 493.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3501) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.83, 79.58) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 42, 139) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 367) [M::mem_pestat] mean and std.dev: (58.90, 79.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 481) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 999.090 CPU sec, 44.499 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 666785, 56, 12) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.69, 79.50) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 33, 89) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 231) [M::mem_pestat] mean and std.dev: (33.67, 24.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 302) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1189, 1526, 1883) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3271) [M::mem_pestat] mean and std.dev: (1155.60, 625.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3965) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1014.429 CPU sec, 59.868 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 666499, 57, 14) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.71, 79.50) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 30, 91) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 245) [M::mem_pestat] mean and std.dev: (34.32, 35.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 322) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (498, 1129, 1367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3105) [M::mem_pestat] mean and std.dev: (1002.77, 495.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3974) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1005.650 CPU sec, 44.560 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 666173, 66, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (281, 764, 1075) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2663) [M::mem_pestat] mean and std.dev: (603.00, 351.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3457) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.71, 79.55) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 35, 89) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 231) [M::mem_pestat] mean and std.dev: (40.09, 39.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 302) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1009.312 CPU sec, 59.112 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 667464, 74, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (247, 795, 1080) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2746) [M::mem_pestat] mean and std.dev: (789.12, 615.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3579) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.69, 79.69) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 34, 613) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1815) [M::mem_pestat] mean and std.dev: (145.27, 376.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2416) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (786, 991, 1099) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (160, 1725) [M::mem_pestat] mean and std.dev: (885.93, 406.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2514) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 998.979 CPU sec, 44.460 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 666836, 45, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.76, 79.58) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 26, 57) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 147) [M::mem_pestat] mean and std.dev: (30.70, 27.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 192) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1002.760 CPU sec, 58.057 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 666969, 69, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (269, 394, 964) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2354) [M::mem_pestat] mean and std.dev: (470.77, 323.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3049) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.56, 79.47) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 33, 107) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 275) [M::mem_pestat] mean and std.dev: (32.54, 24.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 359) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (862, 1442, 2928) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7060) [M::mem_pestat] mean and std.dev: (1736.15, 1063.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9126) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1001.674 CPU sec, 44.724 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 666473, 59, 16) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.84, 79.49) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 34, 75) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 197) [M::mem_pestat] mean and std.dev: (34.33, 30.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 258) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (915, 1175, 1483) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2619) [M::mem_pestat] mean and std.dev: (1102.71, 515.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3187) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1004.166 CPU sec, 57.580 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 666588, 66, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (304, 862, 1740) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4612) [M::mem_pestat] mean and std.dev: (1066.50, 896.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6048) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.89, 79.49) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 32, 59) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 143) [M::mem_pestat] mean and std.dev: (31.35, 22.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 185) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (332, 1330, 1514) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3878) [M::mem_pestat] mean and std.dev: (897.91, 623.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5060) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 998.732 CPU sec, 44.261 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 666396, 56, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (236, 412, 1123) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2897) [M::mem_pestat] mean and std.dev: (617.93, 530.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3784) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.81, 79.42) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 48, 110) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 298) [M::mem_pestat] mean and std.dev: (52.42, 52.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 392) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (416, 878, 1495) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3653) [M::mem_pestat] mean and std.dev: (952.93, 898.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4732) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1003.296 CPU sec, 57.610 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 666662, 64, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (121, 581, 955) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2623) [M::mem_pestat] mean and std.dev: (629.21, 509.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3457) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.81, 79.50) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 39, 114) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 302) [M::mem_pestat] mean and std.dev: (54.43, 65.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 396) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 994.183 CPU sec, 44.174 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 667334, 64, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (296, 872, 1371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3521) [M::mem_pestat] mean and std.dev: (860.94, 681.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4596) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.75, 79.44) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 39, 1289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3825) [M::mem_pestat] mean and std.dev: (502.35, 1025.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5093) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1000.241 CPU sec, 57.818 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 666856, 60, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (273, 305, 1429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3741) [M::mem_pestat] mean and std.dev: (649.00, 620.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4897) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.72, 79.36) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 35, 60) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 148) [M::mem_pestat] mean and std.dev: (35.58, 28.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 192) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (566, 1235, 1704) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3980) [M::mem_pestat] mean and std.dev: (1027.44, 566.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5118) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 993.231 CPU sec, 43.976 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 666338, 65, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (540, 1763, 2561) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6603) [M::mem_pestat] mean and std.dev: (1277.88, 822.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8624) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.74, 79.48) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 41, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 818) [M::mem_pestat] mean and std.dev: (57.53, 115.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1084) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1002.839 CPU sec, 58.293 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 667431, 79, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (271, 539, 823) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1927) [M::mem_pestat] mean and std.dev: (437.10, 262.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2479) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.82, 79.51) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 33, 140) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 392) [M::mem_pestat] mean and std.dev: (35.98, 41.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 518) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (853, 1492, 1797) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3685) [M::mem_pestat] mean and std.dev: (1394.83, 751.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4629) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 995.389 CPU sec, 44.198 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 666506, 64, 10) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.74, 79.48) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 32, 75) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 193) [M::mem_pestat] mean and std.dev: (32.47, 29.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 252) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (307, 941, 1275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3211) [M::mem_pestat] mean and std.dev: (903.20, 713.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4179) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1010.444 CPU sec, 58.144 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 666801, 56, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (401, 796, 1207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2819) [M::mem_pestat] mean and std.dev: (782.88, 499.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3625) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.79, 79.41) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 33, 55) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 141) [M::mem_pestat] mean and std.dev: (24.67, 16.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 184) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1450, 2168, 2570) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4810) [M::mem_pestat] mean and std.dev: (1938.67, 943.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5930) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1002.451 CPU sec, 44.684 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 666113, 78, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (387, 673, 1213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2865) [M::mem_pestat] mean and std.dev: (705.07, 435.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3691) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.72, 79.64) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 31, 98) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 270) [M::mem_pestat] mean and std.dev: (35.59, 38.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 356) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1071, 1205, 2222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4524) [M::mem_pestat] mean and std.dev: (1459.56, 803.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5675) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1003.102 CPU sec, 51.071 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 666602, 52, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (404, 654, 1488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3656) [M::mem_pestat] mean and std.dev: (781.08, 600.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4740) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.80, 79.47) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 35, 93) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 239) [M::mem_pestat] mean and std.dev: (35.50, 26.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 312) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1027, 1365, 2918) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6700) [M::mem_pestat] mean and std.dev: (1504.18, 1188.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8591) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1009.484 CPU sec, 59.417 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 667062, 49, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (466, 718, 1727) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4249) [M::mem_pestat] mean and std.dev: (724.22, 523.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5510) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.82, 79.51) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 28, 78) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 210) [M::mem_pestat] mean and std.dev: (30.85, 33.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 276) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 992.626 CPU sec, 43.961 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 666877, 54, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (531, 694, 1326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2916) [M::mem_pestat] mean and std.dev: (948.46, 640.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3711) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.61, 79.43) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 31, 114) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 300) [M::mem_pestat] mean and std.dev: (41.05, 43.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 393) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (449, 957, 1622) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3968) [M::mem_pestat] mean and std.dev: (1055.92, 783.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5141) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1011.335 CPU sec, 59.107 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 666929, 58, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (302, 876, 1110) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2726) [M::mem_pestat] mean and std.dev: (763.36, 473.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3534) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.90, 79.51) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 39, 69) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 163) [M::mem_pestat] mean and std.dev: (31.13, 20.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 210) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (901, 1011, 3079) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7435) [M::mem_pestat] mean and std.dev: (1600.00, 1432.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9613) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1005.106 CPU sec, 44.774 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 666860, 60, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (330, 786, 2313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6279) [M::mem_pestat] mean and std.dev: (1318.08, 1294.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8262) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.80, 79.73) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 33, 145) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (50.84, 67.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 529) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1008.355 CPU sec, 58.965 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 666881, 62, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (263, 563, 862) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2060) [M::mem_pestat] mean and std.dev: (593.86, 435.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2659) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.72, 79.48) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 33, 79) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 211) [M::mem_pestat] mean and std.dev: (37.98, 40.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 277) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (370, 851, 1607) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4081) [M::mem_pestat] mean and std.dev: (901.20, 639.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5318) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 992.412 CPU sec, 44.073 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 666880, 62, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.74, 79.50) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 37, 104) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 276) [M::mem_pestat] mean and std.dev: (40.51, 43.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 362) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1006.938 CPU sec, 59.232 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 667098, 76, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (610, 997, 4775) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13105) [M::mem_pestat] mean and std.dev: (2007.31, 1803.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17270) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.80, 79.41) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 41, 128) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 344) [M::mem_pestat] mean and std.dev: (50.36, 57.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 452) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 999.709 CPU sec, 44.420 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 665794, 76, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (207, 354, 1435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3891) [M::mem_pestat] mean and std.dev: (735.71, 732.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5119) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (121, 651) [M::mem_pestat] mean and std.dev: (387.04, 79.83) [M::mem_pestat] low and high boundaries for proper pairs: (15, 757) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 31, 96) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 256) [M::mem_pestat] mean and std.dev: (29.97, 27.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 336) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1018.039 CPU sec, 61.026 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 666439, 59, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (306, 670, 1892) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5064) [M::mem_pestat] mean and std.dev: (1194.79, 1208.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6650) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.76, 79.57) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 40, 87) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 231) [M::mem_pestat] mean and std.dev: (38.66, 36.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 303) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1006.657 CPU sec, 44.642 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 666594, 60, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (307, 366, 1699) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4483) [M::mem_pestat] mean and std.dev: (757.42, 641.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5875) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.81, 79.52) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 38, 99) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 265) [M::mem_pestat] mean and std.dev: (41.44, 42.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 348) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (736, 1136, 1461) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2911) [M::mem_pestat] mean and std.dev: (987.75, 433.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3636) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1005.238 CPU sec, 57.973 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 665952, 84, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (403, 853, 1324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3166) [M::mem_pestat] mean and std.dev: (705.22, 440.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4087) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.73, 79.68) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 36, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (53.33, 72.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 622) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (284, 1024, 1405) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3647) [M::mem_pestat] mean and std.dev: (1045.38, 796.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4768) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1006.806 CPU sec, 44.870 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 666997, 61, 10) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.64, 79.62) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 32, 107) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 289) [M::mem_pestat] mean and std.dev: (38.80, 40.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 380) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1416, 1770, 1874) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (500, 2790) [M::mem_pestat] mean and std.dev: (1530.00, 277.41) [M::mem_pestat] low and high boundaries for proper pairs: (42, 3248) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1008.051 CPU sec, 58.229 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 666905, 45, 13) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.83, 79.38) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 39, 1616) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4818) [M::mem_pestat] mean and std.dev: (659.40, 1223.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6419) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (476, 977, 1496) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3536) [M::mem_pestat] mean and std.dev: (1124.54, 753.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4556) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 999.987 CPU sec, 44.504 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666734, 62, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (385, 667, 1009) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2257) [M::mem_pestat] mean and std.dev: (775.73, 444.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2881) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.69, 79.51) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 35, 97) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 261) [M::mem_pestat] mean and std.dev: (37.72, 40.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 343) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (556, 1250, 2973) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7807) [M::mem_pestat] mean and std.dev: (1530.62, 1337.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10224) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1005.066 CPU sec, 57.419 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 667103, 63, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (285, 1386, 1691) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4503) [M::mem_pestat] mean and std.dev: (943.10, 714.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5909) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.64, 79.67) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 33, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 893) [M::mem_pestat] mean and std.dev: (84.06, 183.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1185) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1268, 1602, 2099) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3761) [M::mem_pestat] mean and std.dev: (1498.45, 628.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4592) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1005.551 CPU sec, 44.620 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 666484, 60, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (342, 741, 1327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3297) [M::mem_pestat] mean and std.dev: (878.00, 758.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4282) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.75, 79.46) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 43, 801) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2363) [M::mem_pestat] mean and std.dev: (218.58, 515.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3144) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1005.792 CPU sec, 58.233 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 666743, 69, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (254, 718, 1851) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5045) [M::mem_pestat] mean and std.dev: (946.18, 879.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6642) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.69, 79.59) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 35, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585) [M::mem_pestat] mean and std.dev: (66.03, 80.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 776) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1004.000 CPU sec, 44.805 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 666987, 61, 11) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.76, 79.47) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 40, 417) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1213) [M::mem_pestat] mean and std.dev: (79.45, 184.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1611) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (833, 1092, 1604) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3146) [M::mem_pestat] mean and std.dev: (1333.36, 855.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4756) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1002.240 CPU sec, 58.145 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 666754, 52, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.74, 79.45) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 34, 494) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1454) [M::mem_pestat] mean and std.dev: (84.98, 213.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1934) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1004.089 CPU sec, 44.689 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 666990, 67, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (387, 971, 1300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3126) [M::mem_pestat] mean and std.dev: (937.08, 713.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4039) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.58, 79.43) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 30, 67) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 169) [M::mem_pestat] mean and std.dev: (32.12, 26.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 220) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1000.852 CPU sec, 54.558 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 666838, 57, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (315, 635, 1999) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5367) [M::mem_pestat] mean and std.dev: (968.33, 838.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7051) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.73, 79.54) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 40, 142) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 398) [M::mem_pestat] mean and std.dev: (43.62, 57.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 526) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (970, 1134, 1297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (316, 1951) [M::mem_pestat] mean and std.dev: (1100.83, 108.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2278) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1013.054 CPU sec, 59.574 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 666583, 63, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (278, 1057, 1660) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4424) [M::mem_pestat] mean and std.dev: (1105.75, 1015.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5806) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.85, 79.44) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 37, 102) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 278) [M::mem_pestat] mean and std.dev: (33.84, 31.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 366) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (246, 1135, 2133) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5907) [M::mem_pestat] mean and std.dev: (1260.30, 885.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7794) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1006.008 CPU sec, 44.786 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 666590, 64, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (311, 386, 1268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3182) [M::mem_pestat] mean and std.dev: (708.77, 671.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4139) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.66, 79.62) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 33, 110) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 290) [M::mem_pestat] mean and std.dev: (38.17, 34.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 380) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (738, 1236, 1516) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3072) [M::mem_pestat] mean and std.dev: (1069.38, 491.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3850) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1001.544 CPU sec, 57.541 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 666558, 57, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (312, 449, 758) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1650) [M::mem_pestat] mean and std.dev: (543.56, 393.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2119) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.94, 79.50) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 39, 104) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (36.33, 37.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 368) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (713, 1122, 2713) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6713) [M::mem_pestat] mean and std.dev: (1506.91, 1188.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8713) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1006.650 CPU sec, 44.923 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 667077, 77, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (313, 558, 996) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2362) [M::mem_pestat] mean and std.dev: (585.90, 309.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3045) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.76, 79.48) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 32, 87) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 231) [M::mem_pestat] mean and std.dev: (34.98, 32.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 303) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (903, 1174, 1776) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3522) [M::mem_pestat] mean and std.dev: (1136.36, 705.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4395) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1004.105 CPU sec, 58.325 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 666422, 63, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (646, 1416, 2082) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4954) [M::mem_pestat] mean and std.dev: (1151.36, 738.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6390) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.88, 79.47) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 28, 71) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 183) [M::mem_pestat] mean and std.dev: (32.28, 27.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 239) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1005.937 CPU sec, 44.801 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 666477, 64, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (321, 909, 2254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6120) [M::mem_pestat] mean and std.dev: (1307.43, 1130.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8053) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.79, 79.47) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 28, 54) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 130) [M::mem_pestat] mean and std.dev: (28.89, 22.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 168) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1562, 2143, 3032) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5972) [M::mem_pestat] mean and std.dev: (2107.42, 1028.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7442) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1005.591 CPU sec, 58.385 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 666245, 68, 10) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.79, 79.44) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 42, 108) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 290) [M::mem_pestat] mean and std.dev: (43.84, 45.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 381) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (361, 886, 1017) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2329) [M::mem_pestat] mean and std.dev: (663.44, 361.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2985) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1005.872 CPU sec, 44.775 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 666724, 65, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (324, 407, 1551) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4005) [M::mem_pestat] mean and std.dev: (789.58, 711.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5232) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.67, 79.51) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 44, 113) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 289) [M::mem_pestat] mean and std.dev: (45.30, 42.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 377) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1005.929 CPU sec, 58.746 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 666928, 69, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (528, 1060, 1789) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4311) [M::mem_pestat] mean and std.dev: (1041.87, 644.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5572) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.78, 79.52) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 38, 103) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 271) [M::mem_pestat] mean and std.dev: (41.47, 34.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 355) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1002.768 CPU sec, 44.529 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 666815, 69, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (269, 813, 1313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3401) [M::mem_pestat] mean and std.dev: (849.85, 737.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4445) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.79, 79.54) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 36, 70) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 174) [M::mem_pestat] mean and std.dev: (34.38, 27.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 226) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1315, 1657, 3966) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9268) [M::mem_pestat] mean and std.dev: (2640.38, 2296.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11919) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 992.213 CPU sec, 46.353 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 666641, 69, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (781, 2185, 2968) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7342) [M::mem_pestat] mean and std.dev: (1703.30, 1136.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9529) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.70, 79.44) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 43, 111) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 289) [M::mem_pestat] mean and std.dev: (51.24, 53.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 378) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1011.806 CPU sec, 58.127 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666671, 66, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (301, 361, 966) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2296) [M::mem_pestat] mean and std.dev: (563.82, 345.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2961) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.77, 79.49) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 34, 64) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 154) [M::mem_pestat] mean and std.dev: (31.57, 21.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 199) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (866, 1393, 1758) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3542) [M::mem_pestat] mean and std.dev: (1177.54, 599.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4434) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1001.199 CPU sec, 44.582 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 667080, 61, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (461, 1305, 2585) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6833) [M::mem_pestat] mean and std.dev: (1610.08, 1310.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8957) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.61, 79.58) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 36, 69) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 165) [M::mem_pestat] mean and std.dev: (37.72, 31.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 213) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1009.638 CPU sec, 57.810 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666569, 52, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (381, 414, 1507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3759) [M::mem_pestat] mean and std.dev: (754.60, 509.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4885) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.63, 79.52) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 63, 568) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1650) [M::mem_pestat] mean and std.dev: (119.88, 267.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2191) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (621, 863, 2388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5922) [M::mem_pestat] mean and std.dev: (1273.69, 901.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7689) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1004.923 CPU sec, 44.747 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 666364, 78, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (300, 447, 963) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2289) [M::mem_pestat] mean and std.dev: (556.23, 352.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2952) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.79, 79.51) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 36, 105) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 289) [M::mem_pestat] mean and std.dev: (35.11, 34.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 381) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1079, 1189, 1625) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2717) [M::mem_pestat] mean and std.dev: (1003.80, 582.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3333) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1002.962 CPU sec, 57.192 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 667307, 73, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (192, 417, 1470) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4026) [M::mem_pestat] mean and std.dev: (639.54, 590.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5304) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.72, 79.39) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (11, 25, 63) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 167) [M::mem_pestat] mean and std.dev: (27.03, 22.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 219) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (666, 1024, 1760) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3948) [M::mem_pestat] mean and std.dev: (1055.33, 540.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5042) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1000.559 CPU sec, 44.485 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 666604, 55, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (328, 1132, 1609) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4171) [M::mem_pestat] mean and std.dev: (1127.80, 912.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5452) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.71, 79.45) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 35, 100) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 256) [M::mem_pestat] mean and std.dev: (48.83, 50.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 334) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (639, 941, 1519) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3279) [M::mem_pestat] mean and std.dev: (1137.58, 933.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4873) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1009.391 CPU sec, 59.058 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 667165, 67, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (297, 428, 1234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3108) [M::mem_pestat] mean and std.dev: (752.29, 601.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4045) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.60, 79.41) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 34, 745) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2187) [M::mem_pestat] mean and std.dev: (174.20, 430.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2908) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1030, 1441, 2347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4981) [M::mem_pestat] mean and std.dev: (1555.35, 944.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6298) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 997.112 CPU sec, 44.466 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 666954, 63, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (305, 389, 1526) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3968) [M::mem_pestat] mean and std.dev: (733.76, 658.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5189) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.64, 79.42) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 35, 108) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 294) [M::mem_pestat] mean and std.dev: (45.53, 52.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 387) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (875, 1420, 3220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7910) [M::mem_pestat] mean and std.dev: (2052.38, 1840.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10255) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1010.139 CPU sec, 59.341 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 666356, 68, 13) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.77, 79.51) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (9, 37, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 498) [M::mem_pestat] mean and std.dev: (58.88, 98.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 661) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1008, 1526, 2131) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4377) [M::mem_pestat] mean and std.dev: (1365.62, 727.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5500) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1002.927 CPU sec, 44.688 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 667008, 59, 10) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.71, 79.44) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 37, 99) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 257) [M::mem_pestat] mean and std.dev: (40.25, 37.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 336) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (896, 1490, 2704) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6320) [M::mem_pestat] mean and std.dev: (1691.56, 1307.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8128) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1009.720 CPU sec, 58.633 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 666891, 66, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (304, 740, 1241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3115) [M::mem_pestat] mean and std.dev: (723.43, 464.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4052) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.66, 79.54) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 43, 65) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 155) [M::mem_pestat] mean and std.dev: (36.46, 25.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 200) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 998.403 CPU sec, 44.536 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 666432, 68, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (309, 371, 1986) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5340) [M::mem_pestat] mean and std.dev: (920.08, 857.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7017) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.72, 79.56) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 40, 142) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388) [M::mem_pestat] mean and std.dev: (54.58, 66.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 511) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (710, 1254, 1895) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4265) [M::mem_pestat] mean and std.dev: (1151.82, 891.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5450) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1005.827 CPU sec, 57.171 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 666764, 62, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (339, 622, 1713) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4461) [M::mem_pestat] mean and std.dev: (1101.90, 1035.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5835) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.70, 79.47) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (9, 32, 56) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 150) [M::mem_pestat] mean and std.dev: (28.51, 24.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 197) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (193, 1295, 2143) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6043) [M::mem_pestat] mean and std.dev: (1286.40, 1304.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7993) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1004.961 CPU sec, 44.666 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 667311, 61, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (418, 888, 1178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2698) [M::mem_pestat] mean and std.dev: (712.64, 411.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3458) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.68, 79.55) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (11, 40, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905) [M::mem_pestat] mean and std.dev: (71.14, 155.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1203) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (453, 1050, 1648) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4038) [M::mem_pestat] mean and std.dev: (1018.21, 783.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5233) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1002.873 CPU sec, 57.256 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 666568, 58, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (330, 741, 978) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2274) [M::mem_pestat] mean and std.dev: (709.54, 506.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2922) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.62, 79.47) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 28, 56) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 142) [M::mem_pestat] mean and std.dev: (26.79, 19.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 185) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1007.076 CPU sec, 44.925 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 666538, 82, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (704, 1207, 2160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5072) [M::mem_pestat] mean and std.dev: (1379.08, 1000.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6528) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.66, 79.54) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 35, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 727) [M::mem_pestat] mean and std.dev: (52.45, 87.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 964) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (270, 1207, 2280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6300) [M::mem_pestat] mean and std.dev: (1353.86, 1090.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8310) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1002.123 CPU sec, 57.251 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666904, 56, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (439, 821, 1178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2656) [M::mem_pestat] mean and std.dev: (856.73, 531.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3395) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.75, 79.50) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 41, 136) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 372) [M::mem_pestat] mean and std.dev: (39.61, 40.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 490) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (821, 1112, 1390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2528) [M::mem_pestat] mean and std.dev: (1037.31, 549.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3236) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1006.495 CPU sec, 44.850 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 666850, 76, 13) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.73, 79.44) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 32, 98) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 268) [M::mem_pestat] mean and std.dev: (29.52, 28.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 353) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (332, 986, 1639) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4253) [M::mem_pestat] mean and std.dev: (1150.08, 1020.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5560) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1010.185 CPU sec, 59.629 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 666650, 58, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (284, 1116, 3951) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11285) [M::mem_pestat] mean and std.dev: (1887.57, 2111.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14952) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.93, 79.57) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 47, 530) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1550) [M::mem_pestat] mean and std.dev: (129.90, 269.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2060) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (768, 1031, 1572) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3180) [M::mem_pestat] mean and std.dev: (1064.10, 625.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3984) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1005.696 CPU sec, 44.791 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 666674, 61, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (582, 1294, 1617) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3687) [M::mem_pestat] mean and std.dev: (1040.42, 661.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4722) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.75, 79.70) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 32, 68) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 170) [M::mem_pestat] mean and std.dev: (32.73, 26.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 221) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (912, 1241, 2116) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4524) [M::mem_pestat] mean and std.dev: (1379.31, 614.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5728) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1002.220 CPU sec, 50.341 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 666851, 73, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (528, 1228, 2160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5424) [M::mem_pestat] mean and std.dev: (1268.58, 854.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7056) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.77, 79.43) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 27, 48) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 114) [M::mem_pestat] mean and std.dev: (26.26, 18.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 147) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1009.271 CPU sec, 57.989 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 666916, 57, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.85, 79.61) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 37, 71) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 169) [M::mem_pestat] mean and std.dev: (38.17, 29.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 218) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1007.639 CPU sec, 44.905 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666611, 69, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (344, 382, 1048) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2456) [M::mem_pestat] mean and std.dev: (711.09, 558.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3160) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.77, 79.70) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 32, 83) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 219) [M::mem_pestat] mean and std.dev: (34.97, 32.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 287) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (822, 1373, 4335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11361) [M::mem_pestat] mean and std.dev: (2544.60, 2340.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14874) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1005.509 CPU sec, 57.997 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 666655, 67, 10) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.80, 79.43) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 38, 80) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 212) [M::mem_pestat] mean and std.dev: (31.58, 27.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 278) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (431, 1618, 1899) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4835) [M::mem_pestat] mean and std.dev: (1281.56, 999.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6303) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1004.776 CPU sec, 44.726 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666529, 73, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (468, 743, 1597) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3855) [M::mem_pestat] mean and std.dev: (853.70, 573.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4984) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.71, 79.41) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 41, 131) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 359) [M::mem_pestat] mean and std.dev: (48.87, 61.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 473) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (561, 1235, 1590) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3648) [M::mem_pestat] mean and std.dev: (1113.17, 651.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4677) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1007.107 CPU sec, 58.997 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 666452, 62, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (706, 1145, 2663) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6577) [M::mem_pestat] mean and std.dev: (1971.80, 1925.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9674) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.75, 79.66) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 34, 103) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 279) [M::mem_pestat] mean and std.dev: (43.49, 47.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 367) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1003, 1073, 1582) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2740) [M::mem_pestat] mean and std.dev: (992.18, 324.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3319) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1009.852 CPU sec, 44.881 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 666737, 56, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (273, 978, 1370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3564) [M::mem_pestat] mean and std.dev: (880.15, 628.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4661) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.61, 79.66) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 59, 1924) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5728) [M::mem_pestat] mean and std.dev: (933.29, 1493.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7630) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (656, 1174, 2173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5207) [M::mem_pestat] mean and std.dev: (1364.62, 879.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6724) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1007.103 CPU sec, 58.243 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 667336, 75, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (292, 736, 1422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3682) [M::mem_pestat] mean and std.dev: (1019.74, 851.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4812) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.79, 79.62) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 30, 123) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 333) [M::mem_pestat] mean and std.dev: (35.03, 33.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 438) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (691, 1072, 1770) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3928) [M::mem_pestat] mean and std.dev: (1297.73, 855.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5007) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 997.278 CPU sec, 44.445 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 666564, 76, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (280, 554, 1150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2890) [M::mem_pestat] mean and std.dev: (626.44, 378.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3760) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.52, 79.59) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 42, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (50.15, 59.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 618) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1002.883 CPU sec, 58.116 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 666615, 53, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (235, 311, 843) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2059) [M::mem_pestat] mean and std.dev: (497.13, 341.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2667) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.63, 79.56) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 30, 106) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 290) [M::mem_pestat] mean and std.dev: (44.44, 56.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 382) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (521, 1938, 4608) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12782) [M::mem_pestat] mean and std.dev: (2383.10, 2138.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16869) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1004.461 CPU sec, 44.361 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 666652, 70, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (847, 976, 3025) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7381) [M::mem_pestat] mean and std.dev: (1795.20, 1641.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9559) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.74, 79.70) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 33, 83) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 219) [M::mem_pestat] mean and std.dev: (40.73, 41.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 287) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1008.723 CPU sec, 59.320 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 667026, 71, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (316, 1013, 1330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3358) [M::mem_pestat] mean and std.dev: (1010.17, 879.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4527) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.70, 79.59) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 36, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1498) [M::mem_pestat] mean and std.dev: (75.19, 141.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1993) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1206, 1520, 1664) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (290, 2580) [M::mem_pestat] mean and std.dev: (1423.53, 490.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3387) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 997.936 CPU sec, 44.548 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 666838, 69, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (409, 962, 1893) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4861) [M::mem_pestat] mean and std.dev: (842.21, 629.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6345) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.78, 79.51) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 34, 97) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 253) [M::mem_pestat] mean and std.dev: (33.87, 26.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 331) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1098, 1866, 3596) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8592) [M::mem_pestat] mean and std.dev: (2287.08, 1616.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11090) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1011.323 CPU sec, 59.505 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 666983, 72, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (342, 1074, 1463) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3705) [M::mem_pestat] mean and std.dev: (1030.22, 792.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4826) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.80, 79.51) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 39, 106) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 282) [M::mem_pestat] mean and std.dev: (41.71, 41.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 370) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (702, 1294, 1715) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3741) [M::mem_pestat] mean and std.dev: (1440.57, 875.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4944) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 998.107 CPU sec, 44.569 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 666771, 65, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (360, 725, 1253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3039) [M::mem_pestat] mean and std.dev: (831.15, 629.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3932) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.92, 79.48) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 31, 1076) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3192) [M::mem_pestat] mean and std.dev: (360.34, 780.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4250) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (724, 1577, 1836) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4060) [M::mem_pestat] mean and std.dev: (1190.08, 680.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5172) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1013.230 CPU sec, 58.986 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 666691, 66, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (345, 482, 1176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2838) [M::mem_pestat] mean and std.dev: (868.80, 735.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3811) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.70, 79.55) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 36, 87) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 233) [M::mem_pestat] mean and std.dev: (37.02, 36.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 306) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (591, 1680, 2754) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7080) [M::mem_pestat] mean and std.dev: (2009.38, 2001.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10015) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1005.256 CPU sec, 44.688 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 666528, 62, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (230, 609, 1187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3101) [M::mem_pestat] mean and std.dev: (568.56, 400.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4058) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.73, 79.37) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 42, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628) [M::mem_pestat] mean and std.dev: (51.27, 60.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 831) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (887, 1498, 2635) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6131) [M::mem_pestat] mean and std.dev: (1740.60, 1156.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7879) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1002.675 CPU sec, 57.551 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 666837, 58, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.77, 79.37) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 39, 397) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1155) [M::mem_pestat] mean and std.dev: (97.55, 189.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1534) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1009.332 CPU sec, 45.010 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 666922, 66, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (290, 405, 572) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1136) [M::mem_pestat] mean and std.dev: (383.17, 122.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1418) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.70, 79.47) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 35, 90) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 244) [M::mem_pestat] mean and std.dev: (39.09, 39.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 321) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1010.869 CPU sec, 58.319 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 666817, 63, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.72, 79.50) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 42, 1694) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5044) [M::mem_pestat] mean and std.dev: (769.11, 1337.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6719) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 993.819 CPU sec, 44.068 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 667013, 58, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (299, 1542, 2548) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7046) [M::mem_pestat] mean and std.dev: (1656.14, 1461.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9295) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.65, 79.52) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 35, 154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 430) [M::mem_pestat] mean and std.dev: (56.41, 67.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 568) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1061, 1462, 2325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4853) [M::mem_pestat] mean and std.dev: (1605.94, 826.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6117) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1000.926 CPU sec, 54.653 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 666683, 74, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (343, 1180, 2138) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5728) [M::mem_pestat] mean and std.dev: (1065.87, 806.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7523) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.84, 79.46) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 51, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726) [M::mem_pestat] mean and std.dev: (64.84, 108.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 961) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1004.021 CPU sec, 58.212 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 666793, 74, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (406, 1033, 1931) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4981) [M::mem_pestat] mean and std.dev: (1064.64, 835.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6506) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.58, 79.47) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 33, 71) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 177) [M::mem_pestat] mean and std.dev: (35.95, 33.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 230) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (984, 1118, 1553) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2691) [M::mem_pestat] mean and std.dev: (1231.90, 643.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3805) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 999.679 CPU sec, 44.433 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 666388, 80, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.87, 79.54) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 40, 98) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 254) [M::mem_pestat] mean and std.dev: (39.54, 34.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 332) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1008.387 CPU sec, 57.902 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 666462, 65, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (275, 600, 939) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2267) [M::mem_pestat] mean and std.dev: (648.41, 553.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2931) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.86, 79.52) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 48, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488) [M::mem_pestat] mean and std.dev: (63.30, 82.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 643) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1008, 1230, 1553) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2643) [M::mem_pestat] mean and std.dev: (1101.67, 447.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3188) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1002.040 CPU sec, 44.566 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 667313, 51, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (287, 1566, 2311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6359) [M::mem_pestat] mean and std.dev: (1156.09, 811.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8383) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.72, 79.51) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 30, 120) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 326) [M::mem_pestat] mean and std.dev: (39.61, 55.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 429) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (422, 843, 2410) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6386) [M::mem_pestat] mean and std.dev: (926.75, 757.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8374) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1001.631 CPU sec, 57.388 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 666472, 63, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (306, 923, 1308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3312) [M::mem_pestat] mean and std.dev: (807.33, 502.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4314) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.74, 79.57) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 31, 83) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 219) [M::mem_pestat] mean and std.dev: (27.27, 19.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 287) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (918, 1297, 1880) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3804) [M::mem_pestat] mean and std.dev: (1401.31, 798.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4766) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1002.835 CPU sec, 44.629 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 667075, 75, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (458, 910, 1646) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4022) [M::mem_pestat] mean and std.dev: (956.62, 603.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5210) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.71, 79.38) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 32, 60) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 146) [M::mem_pestat] mean and std.dev: (30.82, 23.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 189) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (834, 1030, 1275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2157) [M::mem_pestat] mean and std.dev: (1027.93, 536.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3173) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1007.510 CPU sec, 58.078 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 666858, 63, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (202, 260, 797) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1987) [M::mem_pestat] mean and std.dev: (582.00, 551.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2787) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.80, 79.43) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 30, 69) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 181) [M::mem_pestat] mean and std.dev: (36.00, 36.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 237) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (741, 1188, 2343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5547) [M::mem_pestat] mean and std.dev: (1415.42, 1150.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7149) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 997.424 CPU sec, 44.215 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 667059, 66, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (345, 764, 1580) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4050) [M::mem_pestat] mean and std.dev: (956.85, 761.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5285) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.68, 79.52) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 30, 66) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 172) [M::mem_pestat] mean and std.dev: (32.07, 30.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 225) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (602, 918, 1392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2972) [M::mem_pestat] mean and std.dev: (892.14, 561.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3762) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1006.821 CPU sec, 58.321 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 667155, 68, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (284, 443, 797) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1823) [M::mem_pestat] mean and std.dev: (493.69, 292.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2336) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.57, 79.66) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 38, 138) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 380) [M::mem_pestat] mean and std.dev: (43.75, 54.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 501) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (920, 1262, 1570) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2870) [M::mem_pestat] mean and std.dev: (1052.38, 574.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3520) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1003.366 CPU sec, 44.333 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666144, 65, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (251, 754, 1390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3668) [M::mem_pestat] mean and std.dev: (862.27, 700.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4807) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.81, 79.51) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 42, 147) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 415) [M::mem_pestat] mean and std.dev: (35.22, 34.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 549) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (451, 586, 1374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3220) [M::mem_pestat] mean and std.dev: (878.88, 557.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4143) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 994.917 CPU sec, 46.434 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 666375, 47, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (277, 730, 1222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3112) [M::mem_pestat] mean and std.dev: (777.00, 534.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4057) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.88, 79.59) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 33, 66) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 158) [M::mem_pestat] mean and std.dev: (36.40, 30.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 204) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1018, 1630, 2874) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6586) [M::mem_pestat] mean and std.dev: (1929.81, 1486.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8442) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1005.429 CPU sec, 57.883 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 666723, 64, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (252, 672, 2125) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5871) [M::mem_pestat] mean and std.dev: (1311.25, 1623.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7805) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.78, 79.46) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 36, 596) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1754) [M::mem_pestat] mean and std.dev: (174.73, 362.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2333) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (902, 1328, 2398) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5390) [M::mem_pestat] mean and std.dev: (1611.62, 1155.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6886) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1005.605 CPU sec, 44.794 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 666158, 61, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (288, 384, 1808) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4848) [M::mem_pestat] mean and std.dev: (1106.71, 1077.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6368) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.64, 79.43) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 34, 68) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 162) [M::mem_pestat] mean and std.dev: (35.74, 26.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 209) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1496, 1770, 2622) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4874) [M::mem_pestat] mean and std.dev: (1666.91, 893.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6000) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1011.856 CPU sec, 58.326 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666987, 73, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (433, 897, 1800) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4534) [M::mem_pestat] mean and std.dev: (1069.18, 688.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5901) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.81, 79.48) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 43, 131) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 357) [M::mem_pestat] mean and std.dev: (46.48, 46.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 470) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (633, 899, 2456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6102) [M::mem_pestat] mean and std.dev: (1288.15, 891.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7925) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 999.455 CPU sec, 44.402 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 667079, 71, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (316, 411, 1207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2989) [M::mem_pestat] mean and std.dev: (634.77, 511.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3880) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.60, 79.42) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 32, 93) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 243) [M::mem_pestat] mean and std.dev: (37.83, 33.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 318) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1011.031 CPU sec, 58.062 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 666731, 62, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (492, 830, 962) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1902) [M::mem_pestat] mean and std.dev: (605.00, 261.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2372) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.63, 79.51) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (11, 36, 82) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 224) [M::mem_pestat] mean and std.dev: (32.90, 28.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 295) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (869, 1433, 2110) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4592) [M::mem_pestat] mean and std.dev: (1327.27, 837.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5833) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1009.789 CPU sec, 44.977 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 666980, 59, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (267, 408, 1134) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2868) [M::mem_pestat] mean and std.dev: (663.67, 450.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3735) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.68, 79.38) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 39, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 621) [M::mem_pestat] mean and std.dev: (51.00, 66.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 821) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1003.697 CPU sec, 56.670 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 666942, 76, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (342, 511, 1905) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5031) [M::mem_pestat] mean and std.dev: (913.68, 949.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6594) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.56, 79.55) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 40, 140) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 392) [M::mem_pestat] mean and std.dev: (42.39, 49.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 518) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (634, 1178, 1478) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3166) [M::mem_pestat] mean and std.dev: (1069.60, 536.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4010) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 999.375 CPU sec, 44.492 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 666731, 59, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (248, 749, 1193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3083) [M::mem_pestat] mean and std.dev: (765.57, 641.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4028) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.88, 79.44) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 38, 104) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 282) [M::mem_pestat] mean and std.dev: (47.73, 56.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 371) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1005.443 CPU sec, 59.355 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 667223, 64, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (717, 1327, 2251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5319) [M::mem_pestat] mean and std.dev: (1276.35, 794.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6853) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.90, 79.48) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 38, 158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 430) [M::mem_pestat] mean and std.dev: (60.28, 80.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 566) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (509, 1035, 2470) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6392) [M::mem_pestat] mean and std.dev: (1431.23, 1121.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8353) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1001.959 CPU sec, 44.681 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 666223, 67, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (168, 322, 1837) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5175) [M::mem_pestat] mean and std.dev: (941.38, 1016.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6844) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.77, 79.60) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 54, 118) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 316) [M::mem_pestat] mean and std.dev: (51.38, 47.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 415) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1250, 1549, 2323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4469) [M::mem_pestat] mean and std.dev: (1572.67, 520.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5542) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1004.090 CPU sec, 57.304 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666955, 59, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (391, 718, 895) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1903) [M::mem_pestat] mean and std.dev: (763.64, 463.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2619) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.74, 79.52) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 30, 81) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 207) [M::mem_pestat] mean and std.dev: (39.75, 37.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 270) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (355, 971, 1260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3070) [M::mem_pestat] mean and std.dev: (776.40, 542.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3975) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 999.181 CPU sec, 44.636 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 667108, 48, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (491, 967, 2626) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6896) [M::mem_pestat] mean and std.dev: (1461.10, 1156.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9031) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.78, 79.39) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (11, 38, 132) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 374) [M::mem_pestat] mean and std.dev: (32.45, 31.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 495) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (965, 1859, 1994) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4052) [M::mem_pestat] mean and std.dev: (1580.67, 906.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5208) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1008.179 CPU sec, 58.960 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 666574, 62, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (246, 1041, 1888) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5172) [M::mem_pestat] mean and std.dev: (743.75, 588.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6814) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.86, 79.57) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 44, 155) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 425) [M::mem_pestat] mean and std.dev: (62.76, 92.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 560) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (543, 781, 1589) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3681) [M::mem_pestat] mean and std.dev: (972.73, 774.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4727) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1000.400 CPU sec, 44.281 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 666355, 75, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (289, 1275, 2078) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5656) [M::mem_pestat] mean and std.dev: (1249.06, 961.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7445) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.72, 79.51) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 38, 124) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 344) [M::mem_pestat] mean and std.dev: (54.26, 56.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 454) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (693, 927, 1375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2739) [M::mem_pestat] mean and std.dev: (946.38, 470.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3421) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1006.598 CPU sec, 58.923 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 666857, 67, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (425, 663, 1236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2858) [M::mem_pestat] mean and std.dev: (738.89, 334.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3669) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.65, 79.67) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 45, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 883) [M::mem_pestat] mean and std.dev: (58.13, 83.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1170) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 999.650 CPU sec, 44.548 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 666983, 60, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (338, 876, 2674) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7346) [M::mem_pestat] mean and std.dev: (1330.47, 1182.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9682) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.71, 79.51) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 40, 150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (48.64, 55.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 549) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1008.471 CPU sec, 57.603 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 666762, 61, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (283, 1042, 1567) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4135) [M::mem_pestat] mean and std.dev: (1135.07, 1032.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5419) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.54, 79.43) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 28, 53) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 131) [M::mem_pestat] mean and std.dev: (24.32, 15.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 170) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 999.537 CPU sec, 44.302 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 666513, 61, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (312, 1062, 1575) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4101) [M::mem_pestat] mean and std.dev: (980.85, 692.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5364) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.65, 79.56) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 40, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492) [M::mem_pestat] mean and std.dev: (62.68, 94.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 650) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1010.128 CPU sec, 58.706 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 667196, 50, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (278, 691, 1274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3266) [M::mem_pestat] mean and std.dev: (800.06, 651.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4262) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.64, 79.57) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (10, 33, 46) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 118) [M::mem_pestat] mean and std.dev: (26.81, 18.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 154) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (766, 1319, 1924) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4240) [M::mem_pestat] mean and std.dev: (1255.00, 734.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5398) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 997.752 CPU sec, 44.473 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 666409, 54, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (286, 363, 1143) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2857) [M::mem_pestat] mean and std.dev: (733.40, 623.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3714) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.77, 79.49) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 23, 57) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 147) [M::mem_pestat] mean and std.dev: (30.28, 29.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 192) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (902, 1160, 1711) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3329) [M::mem_pestat] mean and std.dev: (1243.11, 696.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4138) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1001.080 CPU sec, 49.657 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 666881, 60, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (418, 763, 1658) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4138) [M::mem_pestat] mean and std.dev: (908.50, 706.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5378) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.81, 79.59) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 36, 73) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 183) [M::mem_pestat] mean and std.dev: (37.08, 29.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 238) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1003.643 CPU sec, 58.293 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 666753, 41, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (611, 709, 2407) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5999) [M::mem_pestat] mean and std.dev: (1331.44, 1296.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7795) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.74, 79.43) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (11, 31, 62) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 164) [M::mem_pestat] mean and std.dev: (27.26, 22.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 215) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1001.910 CPU sec, 44.633 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 666549, 61, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (303, 1197, 2615) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7239) [M::mem_pestat] mean and std.dev: (1356.00, 1288.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9551) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.66, 79.60) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 32, 74) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 198) [M::mem_pestat] mean and std.dev: (34.37, 31.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 260) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (840, 1723, 2324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5292) [M::mem_pestat] mean and std.dev: (1669.06, 1185.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6776) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1003.700 CPU sec, 58.480 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 666664, 64, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.73, 79.50) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 41, 154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (60.94, 82.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 544) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1004.855 CPU sec, 44.633 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666838, 56, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (324, 549, 2420) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6612) [M::mem_pestat] mean and std.dev: (1158.27, 1176.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8708) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.76, 79.48) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 35, 61) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 153) [M::mem_pestat] mean and std.dev: (33.74, 29.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 199) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (830, 1226, 2545) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5975) [M::mem_pestat] mean and std.dev: (1554.55, 1105.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7690) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1008.613 CPU sec, 58.765 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 667404, 63, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (238, 621, 1161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3007) [M::mem_pestat] mean and std.dev: (536.53, 369.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3930) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.71, 79.55) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 34, 131) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 367) [M::mem_pestat] mean and std.dev: (37.45, 44.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 485) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (928, 1199, 1802) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3550) [M::mem_pestat] mean and std.dev: (1160.07, 436.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4424) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 998.423 CPU sec, 44.318 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 667352, 58, 11) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.78, 79.53) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 36, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (62.84, 94.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 678) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (709, 1004, 2941) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7405) [M::mem_pestat] mean and std.dev: (1481.80, 1189.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9637) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1005.720 CPU sec, 59.227 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 666760, 73, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (380, 780, 1088) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2504) [M::mem_pestat] mean and std.dev: (776.85, 503.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3212) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.71, 79.36) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 31, 119) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 327) [M::mem_pestat] mean and std.dev: (35.22, 36.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 431) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1006.337 CPU sec, 44.918 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 666928, 58, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (428, 1097, 1560) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3824) [M::mem_pestat] mean and std.dev: (1093.83, 748.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4956) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.70, 79.62) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 28, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1493) [M::mem_pestat] mean and std.dev: (102.34, 264.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1986) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1257, 1709, 5106) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12804) [M::mem_pestat] mean and std.dev: (2600.50, 2153.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16653) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1003.148 CPU sec, 59.565 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 666851, 81, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (290, 357, 1035) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2525) [M::mem_pestat] mean and std.dev: (512.42, 422.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3270) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.76, 79.43) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 35, 71) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 185) [M::mem_pestat] mean and std.dev: (33.75, 26.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 242) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (335, 2398, 5125) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14705) [M::mem_pestat] mean and std.dev: (2710.15, 2396.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19495) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 993.476 CPU sec, 44.094 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 666829, 48, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (325, 919, 1828) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4834) [M::mem_pestat] mean and std.dev: (1077.87, 914.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6337) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.80, 79.55) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 29, 93) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 241) [M::mem_pestat] mean and std.dev: (40.66, 49.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 315) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (899, 1405, 1983) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4151) [M::mem_pestat] mean and std.dev: (1274.33, 646.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5235) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 999.992 CPU sec, 57.956 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 666356, 72, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (362, 672, 1429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3563) [M::mem_pestat] mean and std.dev: (792.00, 613.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4630) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.81, 79.54) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 47, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1120) [M::mem_pestat] mean and std.dev: (78.83, 151.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1488) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 999.932 CPU sec, 44.470 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 666730, 64, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (336, 922, 2086) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5586) [M::mem_pestat] mean and std.dev: (907.64, 702.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7336) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.82, 79.59) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 40, 64) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 158) [M::mem_pestat] mean and std.dev: (33.02, 23.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 205) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 997.549 CPU sec, 57.840 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666996, 65, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (307, 706, 2323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6355) [M::mem_pestat] mean and std.dev: (950.40, 865.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8371) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.80, 79.37) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 42, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641) [M::mem_pestat] mean and std.dev: (65.75, 102.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 849) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (896, 1081, 1682) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3254) [M::mem_pestat] mean and std.dev: (1274.64, 769.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4354) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 996.807 CPU sec, 44.563 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 666941, 64, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (354, 1043, 1598) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4086) [M::mem_pestat] mean and std.dev: (942.33, 789.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5330) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.70, 79.56) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 25, 52) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 130) [M::mem_pestat] mean and std.dev: (28.44, 27.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 169) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 999.553 CPU sec, 58.834 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 666241, 55, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (316, 855, 1322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3334) [M::mem_pestat] mean and std.dev: (794.17, 594.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4340) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.59, 79.64) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 35, 331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953) [M::mem_pestat] mean and std.dev: (114.13, 209.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1264) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 995.229 CPU sec, 44.342 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 666612, 58, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (425, 1072, 1790) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4520) [M::mem_pestat] mean and std.dev: (1132.46, 979.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5885) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.62, 79.34) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 25, 62) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 162) [M::mem_pestat] mean and std.dev: (25.91, 26.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 212) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (301, 1339, 1876) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5026) [M::mem_pestat] mean and std.dev: (1195.82, 859.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6601) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 993.666 CPU sec, 56.383 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666545, 59, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (307, 363, 1626) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4264) [M::mem_pestat] mean and std.dev: (887.80, 1014.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5583) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.75, 79.54) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 30, 99) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 269) [M::mem_pestat] mean and std.dev: (33.19, 38.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 354) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 993.720 CPU sec, 44.084 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 666611, 57, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (264, 815, 1115) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2817) [M::mem_pestat] mean and std.dev: (723.15, 502.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3668) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.60, 79.66) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 36, 131) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 347) [M::mem_pestat] mean and std.dev: (48.46, 52.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 455) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (429, 1448, 1867) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4743) [M::mem_pestat] mean and std.dev: (1365.43, 1172.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6181) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 985.823 CPU sec, 54.776 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 666624, 63, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (409, 945, 1229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2869) [M::mem_pestat] mean and std.dev: (886.93, 516.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3689) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.55, 79.38) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (11, 30, 44) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 110) [M::mem_pestat] mean and std.dev: (27.30, 22.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 143) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (704, 1602, 2142) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5018) [M::mem_pestat] mean and std.dev: (1417.15, 947.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6456) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 989.975 CPU sec, 43.963 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 666998, 53, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (520, 942, 2042) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5086) [M::mem_pestat] mean and std.dev: (1223.94, 1131.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6608) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.88, 79.47) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 36, 104) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 282) [M::mem_pestat] mean and std.dev: (39.60, 36.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 371) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (308, 822, 2384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6536) [M::mem_pestat] mean and std.dev: (1256.79, 1240.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8612) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 990.897 CPU sec, 52.247 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 666828, 70, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (312, 425, 1638) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4290) [M::mem_pestat] mean and std.dev: (880.75, 743.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5616) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.67, 79.54) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 45, 123) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 339) [M::mem_pestat] mean and std.dev: (41.69, 42.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 447) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1006, 1172, 1598) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2782) [M::mem_pestat] mean and std.dev: (1034.38, 371.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3374) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 989.275 CPU sec, 55.965 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 666814, 60, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (359, 1302, 1823) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4751) [M::mem_pestat] mean and std.dev: (1303.88, 965.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6215) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.71, 79.46) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 38, 118) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 324) [M::mem_pestat] mean and std.dev: (43.04, 43.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 427) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1003, 1453, 1974) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3916) [M::mem_pestat] mean and std.dev: (1624.57, 861.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5069) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 987.311 CPU sec, 43.806 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 665882, 71, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (205, 371, 1025) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2665) [M::mem_pestat] mean and std.dev: (573.12, 414.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3485) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.75, 79.53) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 38, 126) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 342) [M::mem_pestat] mean and std.dev: (51.63, 62.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 450) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (751, 1060, 1580) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3238) [M::mem_pestat] mean and std.dev: (1316.30, 703.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4130) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 994.372 CPU sec, 57.249 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 667123, 66, 13) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.70, 79.65) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 38, 134) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 376) [M::mem_pestat] mean and std.dev: (43.94, 51.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 497) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (865, 1031, 1736) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3478) [M::mem_pestat] mean and std.dev: (1192.46, 566.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4349) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 989.464 CPU sec, 43.945 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 667166, 64, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (726, 1191, 2463) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5937) [M::mem_pestat] mean and std.dev: (1643.54, 1183.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7674) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.80, 79.44) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 35, 73) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 195) [M::mem_pestat] mean and std.dev: (31.66, 29.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 256) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (894, 1205, 1936) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4020) [M::mem_pestat] mean and std.dev: (1342.33, 527.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5062) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 980.541 CPU sec, 55.143 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 667008, 78, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (143, 314, 1843) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5243) [M::mem_pestat] mean and std.dev: (775.15, 783.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6943) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.73, 79.66) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 36, 95) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 261) [M::mem_pestat] mean and std.dev: (35.06, 40.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 344) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (748, 1011, 2053) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4663) [M::mem_pestat] mean and std.dev: (1354.87, 856.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5968) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 985.902 CPU sec, 43.651 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 666461, 62, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (348, 544, 1468) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3708) [M::mem_pestat] mean and std.dev: (802.38, 592.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4828) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.83, 79.55) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 41, 73) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 191) [M::mem_pestat] mean and std.dev: (41.26, 36.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 250) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (866, 1288, 1801) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3671) [M::mem_pestat] mean and std.dev: (1120.71, 643.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4606) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 988.295 CPU sec, 54.156 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 666415, 61, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (526, 962, 1701) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4051) [M::mem_pestat] mean and std.dev: (1171.89, 893.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5226) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.61, 79.52) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 41, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 457) [M::mem_pestat] mean and std.dev: (32.04, 30.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 605) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 986.918 CPU sec, 43.737 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 666658, 64, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (363, 719, 887) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1935) [M::mem_pestat] mean and std.dev: (701.21, 479.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2620) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.72, 79.48) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 29, 61) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 157) [M::mem_pestat] mean and std.dev: (29.64, 29.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 205) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (969, 1440, 1857) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3633) [M::mem_pestat] mean and std.dev: (1382.92, 714.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4521) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 990.059 CPU sec, 55.721 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 666411, 70, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (443, 804, 1014) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2156) [M::mem_pestat] mean and std.dev: (647.38, 240.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2727) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.90, 79.59) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 46, 132) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 372) [M::mem_pestat] mean and std.dev: (42.04, 55.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 492) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (834, 1179, 1984) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4284) [M::mem_pestat] mean and std.dev: (1237.29, 697.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5434) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 990.654 CPU sec, 44.037 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 666375, 78, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (365, 895, 2627) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7151) [M::mem_pestat] mean and std.dev: (1429.78, 1169.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9413) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.90, 79.48) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 28, 103) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 281) [M::mem_pestat] mean and std.dev: (41.05, 47.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 370) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (651, 1234, 1388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2862) [M::mem_pestat] mean and std.dev: (955.56, 415.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3599) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 984.989 CPU sec, 46.178 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 666962, 54, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (296, 756, 1121) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2771) [M::mem_pestat] mean and std.dev: (672.60, 425.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3596) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.69, 79.55) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 31, 60) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 148) [M::mem_pestat] mean and std.dev: (32.87, 27.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 192) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (594, 767, 1538) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3426) [M::mem_pestat] mean and std.dev: (1042.17, 760.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4370) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 990.729 CPU sec, 55.668 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 666872, 59, 14) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.75, 79.47) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 36, 136) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 370) [M::mem_pestat] mean and std.dev: (39.74, 37.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 487) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (222, 661, 1381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3699) [M::mem_pestat] mean and std.dev: (772.36, 597.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4858) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 989.985 CPU sec, 43.757 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 666618, 57, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (354, 805, 1300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3192) [M::mem_pestat] mean and std.dev: (831.50, 507.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4138) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.70, 79.48) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 35, 133) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 361) [M::mem_pestat] mean and std.dev: (44.74, 66.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 475) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (582, 1220, 1393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3015) [M::mem_pestat] mean and std.dev: (1082.50, 677.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3826) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 984.938 CPU sec, 55.538 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 666938, 54, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (355, 845, 1368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3394) [M::mem_pestat] mean and std.dev: (1089.06, 897.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4679) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.71, 79.49) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (11, 34, 60) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 158) [M::mem_pestat] mean and std.dev: (29.60, 26.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 207) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (463, 835, 1083) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2323) [M::mem_pestat] mean and std.dev: (750.18, 428.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2943) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 992.409 CPU sec, 44.085 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 666855, 67, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.82, 79.46) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 29, 81) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 211) [M::mem_pestat] mean and std.dev: (36.92, 34.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 276) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 986.878 CPU sec, 55.133 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 666448, 61, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (271, 657, 1073) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2677) [M::mem_pestat] mean and std.dev: (626.08, 479.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3479) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.54, 79.69) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 45, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585) [M::mem_pestat] mean and std.dev: (68.92, 91.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 773) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (836, 1846, 3012) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7364) [M::mem_pestat] mean and std.dev: (2094.09, 1611.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9540) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 990.845 CPU sec, 43.900 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 666961, 57, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (283, 766, 1729) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4621) [M::mem_pestat] mean and std.dev: (923.30, 693.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6067) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.88, 79.64) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 37, 80) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 210) [M::mem_pestat] mean and std.dev: (30.96, 25.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 275) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (900, 1198, 1672) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3216) [M::mem_pestat] mean and std.dev: (1154.45, 705.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3988) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 989.119 CPU sec, 54.924 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 667075, 54, 14) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.85, 79.51) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 37, 106) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (53.38, 57.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 367) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (362, 961, 1381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3419) [M::mem_pestat] mean and std.dev: (901.85, 581.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4438) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 989.586 CPU sec, 43.834 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 666963, 65, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (277, 731, 937) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2257) [M::mem_pestat] mean and std.dev: (608.00, 324.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2917) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.81, 79.54) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 37, 351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1013) [M::mem_pestat] mean and std.dev: (85.39, 167.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1344) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 995.461 CPU sec, 55.301 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 666373, 65, 13) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.80, 79.63) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 35, 68) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 168) [M::mem_pestat] mean and std.dev: (29.24, 18.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 218) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (639, 1343, 1763) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4011) [M::mem_pestat] mean and std.dev: (1436.62, 939.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5193) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 987.274 CPU sec, 43.725 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 666389, 65, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.85, 79.59) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (11, 33, 71) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 191) [M::mem_pestat] mean and std.dev: (31.63, 30.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 251) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 983.574 CPU sec, 55.740 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 667302, 77, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (284, 571, 1620) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4292) [M::mem_pestat] mean and std.dev: (605.10, 517.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5628) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.70, 79.52) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 25, 53) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 133) [M::mem_pestat] mean and std.dev: (27.22, 22.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 173) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 981.696 CPU sec, 43.652 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 666805, 61, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (362, 963, 3673) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10295) [M::mem_pestat] mean and std.dev: (1853.18, 2002.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13606) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.81, 79.42) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 32, 98) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 258) [M::mem_pestat] mean and std.dev: (37.50, 38.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 338) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (679, 1009, 2278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5476) [M::mem_pestat] mean and std.dev: (1238.92, 763.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7075) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 988.991 CPU sec, 55.502 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666953, 57, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (536, 591, 1324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2900) [M::mem_pestat] mean and std.dev: (811.70, 606.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3688) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.84, 79.51) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 34, 117) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 315) [M::mem_pestat] mean and std.dev: (43.30, 55.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 414) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 990.479 CPU sec, 43.921 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 666609, 73, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (714, 1191, 1869) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4179) [M::mem_pestat] mean and std.dev: (1169.61, 821.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5334) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.97, 79.51) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 36, 83) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 217) [M::mem_pestat] mean and std.dev: (33.14, 28.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 284) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1138, 1497, 3032) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6820) [M::mem_pestat] mean and std.dev: (1724.93, 1126.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8714) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 984.103 CPU sec, 56.101 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 667176, 72, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (362, 852, 1661) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4259) [M::mem_pestat] mean and std.dev: (1074.60, 1030.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5558) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.74, 79.53) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 29, 101) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 279) [M::mem_pestat] mean and std.dev: (30.74, 31.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 368) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (569, 1410, 2180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5402) [M::mem_pestat] mean and std.dev: (1415.46, 1139.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7013) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 985.391 CPU sec, 43.810 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 666986, 70, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (212, 429, 1553) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4235) [M::mem_pestat] mean and std.dev: (953.29, 974.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5576) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.69, 79.64) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 48, 138) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378) [M::mem_pestat] mean and std.dev: (43.44, 39.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 498) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1137, 1615, 2070) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3936) [M::mem_pestat] mean and std.dev: (1482.64, 731.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4869) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 985.761 CPU sec, 55.722 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 666999, 55, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.63, 79.35) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 23, 49) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 123) [M::mem_pestat] mean and std.dev: (25.27, 20.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 160) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 990.272 CPU sec, 43.986 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 667222, 63, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (331, 799, 1369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3445) [M::mem_pestat] mean and std.dev: (748.71, 514.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4483) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.83, 79.47) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 37, 122) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 332) [M::mem_pestat] mean and std.dev: (49.69, 64.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 437) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 983.147 CPU sec, 55.407 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 666581, 61, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (378, 1063, 1779) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4581) [M::mem_pestat] mean and std.dev: (1118.88, 779.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5982) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.73, 79.43) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 43, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1224) [M::mem_pestat] mean and std.dev: (59.00, 93.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1623) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 995.072 CPU sec, 44.221 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 666168, 61, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (303, 864, 1427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3675) [M::mem_pestat] mean and std.dev: (784.17, 577.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4799) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.71, 79.41) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 44, 433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1265) [M::mem_pestat] mean and std.dev: (112.52, 241.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1681) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 990.804 CPU sec, 49.222 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 667080, 65, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (237, 876, 1819) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4983) [M::mem_pestat] mean and std.dev: (985.69, 948.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6565) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.63, 79.47) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 36, 333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 965) [M::mem_pestat] mean and std.dev: (76.32, 119.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1281) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (481, 943, 2060) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5218) [M::mem_pestat] mean and std.dev: (1393.23, 1316.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6797) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 992.511 CPU sec, 57.521 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 666894, 65, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (300, 581, 1125) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2775) [M::mem_pestat] mean and std.dev: (653.44, 516.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3600) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.68, 79.60) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (10, 20, 58) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 154) [M::mem_pestat] mean and std.dev: (21.93, 18.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 202) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 992.585 CPU sec, 43.993 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 666903, 75, 17) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.50, 79.70) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 35, 66) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 162) [M::mem_pestat] mean and std.dev: (33.24, 25.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 210) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (650, 1383, 2459) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6077) [M::mem_pestat] mean and std.dev: (1375.56, 905.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7886) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 990.897 CPU sec, 56.865 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666703, 73, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (279, 526, 818) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1896) [M::mem_pestat] mean and std.dev: (559.40, 387.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2435) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.64, 79.39) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 32, 100) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 266) [M::mem_pestat] mean and std.dev: (43.15, 47.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 349) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (706, 903, 1938) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4402) [M::mem_pestat] mean and std.dev: (1330.53, 1010.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5634) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 988.580 CPU sec, 43.935 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 665942, 57, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (358, 925, 2171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5797) [M::mem_pestat] mean and std.dev: (1350.89, 1247.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7610) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.69, 79.61) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 33, 60) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 148) [M::mem_pestat] mean and std.dev: (30.92, 22.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 192) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (942, 1312, 2006) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4134) [M::mem_pestat] mean and std.dev: (1462.07, 920.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5198) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 993.071 CPU sec, 57.243 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 666443, 44, 11) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.72, 79.59) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 49, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (49.46, 50.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 647) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (841, 1017, 1642) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3244) [M::mem_pestat] mean and std.dev: (1164.36, 821.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4450) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 992.347 CPU sec, 44.187 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 666873, 55, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (306, 449, 1354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3450) [M::mem_pestat] mean and std.dev: (801.93, 645.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4498) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.66, 79.70) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 34, 105) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 283) [M::mem_pestat] mean and std.dev: (32.98, 30.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 372) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (955, 1405, 2658) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6064) [M::mem_pestat] mean and std.dev: (1510.18, 1006.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7767) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 989.582 CPU sec, 54.568 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 666262, 57, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (384, 1119, 2001) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5235) [M::mem_pestat] mean and std.dev: (1178.00, 930.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6852) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.74, 79.68) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (10, 27, 69) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 187) [M::mem_pestat] mean and std.dev: (31.73, 32.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 246) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 991.083 CPU sec, 43.948 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 666883, 60, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (324, 695, 953) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2211) [M::mem_pestat] mean and std.dev: (740.29, 602.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3149) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.72, 79.60) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 34, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 523) [M::mem_pestat] mean and std.dev: (53.49, 85.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 693) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (399, 856, 1639) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4119) [M::mem_pestat] mean and std.dev: (871.58, 536.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5359) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 993.140 CPU sec, 55.572 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 666906, 76, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (457, 893, 2371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6199) [M::mem_pestat] mean and std.dev: (1269.82, 1641.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8113) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.67, 79.77) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 44, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820) [M::mem_pestat] mean and std.dev: (92.08, 153.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1086) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 989.987 CPU sec, 43.845 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 667036, 67, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (310, 534, 1385) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3535) [M::mem_pestat] mean and std.dev: (712.90, 532.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4610) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (121, 651) [M::mem_pestat] mean and std.dev: (386.94, 79.65) [M::mem_pestat] low and high boundaries for proper pairs: (15, 757) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 36, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462) [M::mem_pestat] mean and std.dev: (38.42, 37.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 608) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (583, 1230, 2105) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5149) [M::mem_pestat] mean and std.dev: (1377.40, 956.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6671) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 986.905 CPU sec, 54.241 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 666753, 70, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (369, 1077, 1215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2907) [M::mem_pestat] mean and std.dev: (903.28, 591.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3753) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.74, 79.41) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 50, 145) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 385) [M::mem_pestat] mean and std.dev: (60.02, 61.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 505) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1031, 1209, 3678) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8972) [M::mem_pestat] mean and std.dev: (1969.91, 1622.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11619) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 988.443 CPU sec, 43.884 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 666890, 77, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 698, 1597) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4489) [M::mem_pestat] mean and std.dev: (591.70, 507.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5935) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.74, 79.61) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 39, 74) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 188) [M::mem_pestat] mean and std.dev: (37.23, 33.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 245) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 989.520 CPU sec, 55.418 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 666444, 56, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (351, 1382, 2315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6243) [M::mem_pestat] mean and std.dev: (1562.86, 1293.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8207) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.62, 79.67) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 32, 126) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 354) [M::mem_pestat] mean and std.dev: (34.73, 37.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 468) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (901, 1325, 1748) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3442) [M::mem_pestat] mean and std.dev: (1269.18, 549.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4289) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 989.224 CPU sec, 44.006 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 666538, 63, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (425, 938, 1798) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4544) [M::mem_pestat] mean and std.dev: (969.64, 639.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5917) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.66, 79.46) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 37, 104) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 278) [M::mem_pestat] mean and std.dev: (38.76, 34.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 365) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 986.607 CPU sec, 55.599 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 666587, 58, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (200, 582, 1107) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2921) [M::mem_pestat] mean and std.dev: (602.81, 523.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3828) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.75, 79.40) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 32, 56) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 134) [M::mem_pestat] mean and std.dev: (31.72, 23.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 173) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (285, 1130, 1493) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3909) [M::mem_pestat] mean and std.dev: (1199.40, 1052.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5408) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 988.968 CPU sec, 43.819 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 666821, 64, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (273, 393, 1647) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4395) [M::mem_pestat] mean and std.dev: (835.88, 701.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5769) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.77, 79.82) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 31, 69) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 183) [M::mem_pestat] mean and std.dev: (33.93, 31.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 240) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1156, 1576, 2339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4705) [M::mem_pestat] mean and std.dev: (1863.45, 1300.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7067) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 986.498 CPU sec, 54.157 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 666750, 58, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (265, 403, 1390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3640) [M::mem_pestat] mean and std.dev: (664.54, 493.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4765) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.77, 79.47) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 27, 46) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 114) [M::mem_pestat] mean and std.dev: (23.51, 15.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 148) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 988.491 CPU sec, 43.855 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 666733, 69, 12) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.74, 79.45) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 32, 101) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 271) [M::mem_pestat] mean and std.dev: (34.76, 33.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 356) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (942, 1598, 1763) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3405) [M::mem_pestat] mean and std.dev: (1353.64, 705.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4226) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 989.450 CPU sec, 53.098 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 666967, 57, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (391, 416, 1491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3691) [M::mem_pestat] mean and std.dev: (740.67, 542.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4791) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.71, 79.45) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 41, 962) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2848) [M::mem_pestat] mean and std.dev: (292.45, 583.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3791) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (372, 799, 2454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6618) [M::mem_pestat] mean and std.dev: (1478.00, 1559.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8700) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 991.041 CPU sec, 43.864 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 666619, 57, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (228, 1049, 2483) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6993) [M::mem_pestat] mean and std.dev: (1183.92, 1041.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9248) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.88, 79.56) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 36, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 538) [M::mem_pestat] mean and std.dev: (58.88, 83.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 712) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 993.288 CPU sec, 52.649 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 666210, 61, 13) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.69, 79.39) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 29, 110) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 298) [M::mem_pestat] mean and std.dev: (30.12, 28.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 392) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (866, 962, 1594) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3050) [M::mem_pestat] mean and std.dev: (1165.83, 570.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3778) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1014.438 CPU sec, 58.914 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 667206, 70, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (137, 332, 1164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3218) [M::mem_pestat] mean and std.dev: (579.57, 517.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4245) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.73, 79.41) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 35, 81) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 213) [M::mem_pestat] mean and std.dev: (33.23, 28.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 279) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (678, 1157, 1826) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4122) [M::mem_pestat] mean and std.dev: (991.18, 591.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5270) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1000.467 CPU sec, 44.515 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 666523, 61, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (191, 697, 1068) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2822) [M::mem_pestat] mean and std.dev: (686.30, 528.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3699) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.71, 79.63) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 31, 92) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 252) [M::mem_pestat] mean and std.dev: (35.00, 37.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 332) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1002.528 CPU sec, 58.634 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 666686, 60, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (183, 639, 819) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2091) [M::mem_pestat] mean and std.dev: (582.80, 427.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2727) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.75, 79.61) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (11, 30, 84) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 230) [M::mem_pestat] mean and std.dev: (28.92, 29.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 303) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 993.996 CPU sec, 44.190 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 667163, 66, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (243, 753, 1226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3192) [M::mem_pestat] mean and std.dev: (779.00, 709.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4175) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (121, 651) [M::mem_pestat] mean and std.dev: (386.96, 79.79) [M::mem_pestat] low and high boundaries for proper pairs: (15, 757) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 37, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939) [M::mem_pestat] mean and std.dev: (49.56, 86.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1246) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1133, 1409, 2138) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4148) [M::mem_pestat] mean and std.dev: (1325.25, 470.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5153) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1000.067 CPU sec, 58.312 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 666993, 61, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (264, 714, 1210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3102) [M::mem_pestat] mean and std.dev: (791.00, 740.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4048) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.77, 79.51) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 32, 53) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 133) [M::mem_pestat] mean and std.dev: (31.20, 25.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 173) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (510, 1462, 1696) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4068) [M::mem_pestat] mean and std.dev: (1195.38, 669.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5254) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 996.011 CPU sec, 44.435 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 667573, 78, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (750, 1813, 3520) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9060) [M::mem_pestat] mean and std.dev: (2091.78, 1734.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11830) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.73, 79.34) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 38, 84) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 208) [M::mem_pestat] mean and std.dev: (33.74, 25.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 270) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1018, 1156, 2483) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5413) [M::mem_pestat] mean and std.dev: (1610.73, 1225.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6878) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 994.020 CPU sec, 57.469 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 666525, 66, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (502, 1062, 2014) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5038) [M::mem_pestat] mean and std.dev: (1119.85, 757.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6550) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.52, 79.41) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 33, 58) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 144) [M::mem_pestat] mean and std.dev: (27.74, 18.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 187) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (728, 1279, 2503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6053) [M::mem_pestat] mean and std.dev: (1426.15, 853.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7828) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1000.265 CPU sec, 44.485 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666506, 56, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (246, 879, 2553) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7167) [M::mem_pestat] mean and std.dev: (1562.55, 1718.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9474) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.78, 79.53) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 28, 106) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 292) [M::mem_pestat] mean and std.dev: (36.46, 42.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 385) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1002.434 CPU sec, 57.999 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 666826, 66, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (215, 731, 1006) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2588) [M::mem_pestat] mean and std.dev: (629.08, 393.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3379) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.62, 79.45) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 36, 148) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (45.85, 65.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 547) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1295, 1596, 2892) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6086) [M::mem_pestat] mean and std.dev: (1877.00, 918.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7683) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 997.881 CPU sec, 44.320 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 666804, 65, 17) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.74, 79.61) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 31, 131) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 363) [M::mem_pestat] mean and std.dev: (42.93, 55.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 479) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (320, 872, 1561) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4043) [M::mem_pestat] mean and std.dev: (947.47, 683.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5284) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 987.420 CPU sec, 46.609 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 666045, 61, 14) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.68, 79.39) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 34, 99) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 261) [M::mem_pestat] mean and std.dev: (38.43, 39.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 342) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (791, 1107, 1454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2780) [M::mem_pestat] mean and std.dev: (1160.29, 547.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3443) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1006.470 CPU sec, 57.782 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 666155, 69, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (345, 717, 1555) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3975) [M::mem_pestat] mean and std.dev: (932.57, 736.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5185) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.74, 79.56) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 33, 115) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 321) [M::mem_pestat] mean and std.dev: (38.45, 51.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 424) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (310, 1355, 2332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6376) [M::mem_pestat] mean and std.dev: (1482.10, 1179.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8398) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 996.485 CPU sec, 44.241 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 666494, 64, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (321, 480, 1096) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2646) [M::mem_pestat] mean and std.dev: (719.53, 539.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3421) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.71, 79.53) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 38, 79) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 187) [M::mem_pestat] mean and std.dev: (36.87, 23.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 241) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (287, 637, 3008) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8450) [M::mem_pestat] mean and std.dev: (1734.64, 2289.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11171) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1004.227 CPU sec, 57.868 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 666675, 77, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (261, 403, 977) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2409) [M::mem_pestat] mean and std.dev: (626.93, 506.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3125) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.79, 79.63) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 30, 72) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 184) [M::mem_pestat] mean and std.dev: (33.86, 29.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 240) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (755, 1042, 1474) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2912) [M::mem_pestat] mean and std.dev: (1117.08, 666.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3785) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1002.353 CPU sec, 44.822 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 666689, 70, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (390, 686, 1038) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2334) [M::mem_pestat] mean and std.dev: (704.75, 390.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2982) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.75, 79.58) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 32, 71) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 179) [M::mem_pestat] mean and std.dev: (32.00, 28.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 233) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (370, 1031, 1285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3115) [M::mem_pestat] mean and std.dev: (1037.85, 794.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4217) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1001.347 CPU sec, 58.176 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 666974, 70, 11) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.70, 79.46) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 40, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (50.28, 69.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1140) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (918, 949, 2803) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6573) [M::mem_pestat] mean and std.dev: (1758.09, 1497.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8458) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 995.036 CPU sec, 44.217 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 666645, 56, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (312, 665, 1805) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4791) [M::mem_pestat] mean and std.dev: (1000.72, 846.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6284) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.65, 79.60) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 33, 75) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 199) [M::mem_pestat] mean and std.dev: (34.66, 33.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 261) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (959, 1889, 2765) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6377) [M::mem_pestat] mean and std.dev: (1671.73, 1063.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8183) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1000.296 CPU sec, 59.029 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 666188, 65, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (390, 1119, 1883) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4869) [M::mem_pestat] mean and std.dev: (984.07, 730.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6362) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.66, 79.57) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 38, 140) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 390) [M::mem_pestat] mean and std.dev: (39.40, 45.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 515) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (663, 899, 1561) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3357) [M::mem_pestat] mean and std.dev: (1056.45, 543.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4255) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1005.509 CPU sec, 44.745 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 666260, 58, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (350, 686, 809) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1727) [M::mem_pestat] mean and std.dev: (538.93, 278.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2186) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.74, 79.41) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 37, 77) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 189) [M::mem_pestat] mean and std.dev: (32.69, 22.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 245) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (853, 1094, 1255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (49, 2059) [M::mem_pestat] mean and std.dev: (1033.44, 476.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2941) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1008.836 CPU sec, 60.302 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 667020, 68, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (419, 1116, 2457) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6533) [M::mem_pestat] mean and std.dev: (1302.18, 1415.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8571) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.77, 79.49) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 34, 87) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 227) [M::mem_pestat] mean and std.dev: (34.19, 33.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 297) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (687, 1523, 1695) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3711) [M::mem_pestat] mean and std.dev: (1138.58, 552.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4719) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 998.927 CPU sec, 44.562 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666510, 48, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (337, 417, 2253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6085) [M::mem_pestat] mean and std.dev: (923.10, 806.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8001) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.69, 79.65) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 48, 132) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 364) [M::mem_pestat] mean and std.dev: (46.69, 47.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 480) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1006.757 CPU sec, 58.618 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 666465, 64, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (290, 992, 1479) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3857) [M::mem_pestat] mean and std.dev: (986.80, 600.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5046) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.68, 79.45) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 36, 96) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 250) [M::mem_pestat] mean and std.dev: (42.56, 47.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 327) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (909, 1002, 1853) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3741) [M::mem_pestat] mean and std.dev: (1135.86, 701.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4685) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 998.529 CPU sec, 44.388 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 666542, 59, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (225, 501, 851) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2103) [M::mem_pestat] mean and std.dev: (637.60, 550.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2839) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.59, 79.64) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 26, 91) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 247) [M::mem_pestat] mean and std.dev: (39.69, 53.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 325) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1007.833 CPU sec, 60.133 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 666643, 66, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (418, 990, 1724) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4336) [M::mem_pestat] mean and std.dev: (1005.82, 635.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5642) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.62, 79.52) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 30, 105) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 289) [M::mem_pestat] mean and std.dev: (40.64, 53.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 381) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (701, 994, 1536) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3206) [M::mem_pestat] mean and std.dev: (1153.53, 789.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4311) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 995.171 CPU sec, 44.157 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 667078, 61, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (303, 446, 1062) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2580) [M::mem_pestat] mean and std.dev: (702.86, 642.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3339) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.89, 79.50) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 30, 96) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 260) [M::mem_pestat] mean and std.dev: (29.79, 32.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 342) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (873, 1137, 1706) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3372) [M::mem_pestat] mean and std.dev: (1224.17, 754.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4240) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 999.863 CPU sec, 57.903 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 667257, 60, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (369, 526, 1290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3132) [M::mem_pestat] mean and std.dev: (756.90, 570.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4053) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.75, 79.45) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 29, 50) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 122) [M::mem_pestat] mean and std.dev: (28.47, 21.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 158) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (538, 1486, 1983) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4873) [M::mem_pestat] mean and std.dev: (1347.33, 1121.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6318) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1004.193 CPU sec, 44.604 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 666332, 61, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (543, 1072, 2459) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6291) [M::mem_pestat] mean and std.dev: (1302.06, 1097.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8207) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.78, 79.51) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 35, 128) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 356) [M::mem_pestat] mean and std.dev: (40.94, 52.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 470) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1004.498 CPU sec, 58.282 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 666670, 67, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (345, 990, 1233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3009) [M::mem_pestat] mean and std.dev: (710.58, 413.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3897) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 383, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (387.00, 79.63) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 39, 67) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 153) [M::mem_pestat] mean and std.dev: (40.97, 31.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 196) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1185, 1511, 2631) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5523) [M::mem_pestat] mean and std.dev: (1790.91, 953.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6969) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 994.980 CPU sec, 44.158 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 666599, 78, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (280, 591, 2237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6151) [M::mem_pestat] mean and std.dev: (973.85, 935.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8108) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.85, 79.55) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (11, 37, 146) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (50.12, 73.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 551) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (603, 913, 2701) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6897) [M::mem_pestat] mean and std.dev: (1313.00, 990.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8995) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1006.926 CPU sec, 58.527 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 666595, 71, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (275, 565, 1786) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4808) [M::mem_pestat] mean and std.dev: (1099.40, 1105.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6319) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.74, 79.58) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 29, 55) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 129) [M::mem_pestat] mean and std.dev: (29.67, 20.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 166) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (539, 1184, 2476) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6350) [M::mem_pestat] mean and std.dev: (1412.40, 1313.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8287) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 997.965 CPU sec, 44.146 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 666869, 62, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (248, 310, 977) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2435) [M::mem_pestat] mean and std.dev: (384.50, 284.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3164) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.82, 79.53) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 33, 66) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 162) [M::mem_pestat] mean and std.dev: (34.27, 28.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 210) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 990.164 CPU sec, 47.546 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 666938, 69, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (254, 1081, 1589) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4259) [M::mem_pestat] mean and std.dev: (811.33, 606.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5594) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.88, 79.45) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 43, 541) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1593) [M::mem_pestat] mean and std.dev: (96.48, 249.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2119) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (503, 981, 1718) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4148) [M::mem_pestat] mean and std.dev: (1196.38, 846.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5363) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1002.508 CPU sec, 57.715 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 666747, 78, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (373, 675, 1304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3166) [M::mem_pestat] mean and std.dev: (871.86, 779.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4097) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.77, 79.52) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 36, 64) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 156) [M::mem_pestat] mean and std.dev: (31.69, 22.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 202) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1101, 1496, 2334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4800) [M::mem_pestat] mean and std.dev: (1542.44, 910.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6033) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1004.291 CPU sec, 44.688 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 666962, 57, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (336, 875, 1550) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3978) [M::mem_pestat] mean and std.dev: (691.46, 516.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5192) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.77, 79.51) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 33, 54) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 130) [M::mem_pestat] mean and std.dev: (32.22, 25.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 168) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1003.850 CPU sec, 58.715 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 666037, 57, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (325, 690, 1078) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2584) [M::mem_pestat] mean and std.dev: (674.25, 396.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3337) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.60, 79.45) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 34, 114) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 318) [M::mem_pestat] mean and std.dev: (44.59, 54.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 420) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (436, 1019, 1287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2989) [M::mem_pestat] mean and std.dev: (918.55, 490.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3840) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 997.868 CPU sec, 44.361 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 667005, 70, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (211, 891, 996) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2566) [M::mem_pestat] mean and std.dev: (699.20, 437.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3351) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.84, 79.51) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (11, 30, 57) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 149) [M::mem_pestat] mean and std.dev: (27.19, 23.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 195) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 993.343 CPU sec, 57.034 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 667232, 76, 12) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.67, 79.51) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 35, 62) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 156) [M::mem_pestat] mean and std.dev: (32.84, 26.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 203) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (173, 848, 1055) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2819) [M::mem_pestat] mean and std.dev: (537.91, 407.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3701) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 996.567 CPU sec, 44.520 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 666164, 81, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (397, 811, 1647) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4147) [M::mem_pestat] mean and std.dev: (901.69, 609.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5397) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.82, 79.58) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 29, 72) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 188) [M::mem_pestat] mean and std.dev: (31.09, 27.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 246) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (571, 1090, 1310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2788) [M::mem_pestat] mean and std.dev: (949.25, 637.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3527) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1003.450 CPU sec, 58.865 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 667099, 56, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (327, 978, 1632) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4242) [M::mem_pestat] mean and std.dev: (1073.30, 830.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5547) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.74, 79.61) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 41, 88) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 220) [M::mem_pestat] mean and std.dev: (36.68, 26.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 286) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 997.004 CPU sec, 44.700 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 666698, 60, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.68, 79.53) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 34, 95) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 261) [M::mem_pestat] mean and std.dev: (39.86, 48.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 344) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 993.715 CPU sec, 56.072 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 666250, 81, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (208, 326, 1055) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2749) [M::mem_pestat] mean and std.dev: (527.94, 477.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3596) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.83, 79.44) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 28, 62) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 154) [M::mem_pestat] mean and std.dev: (32.38, 28.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 200) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (693, 1124, 1530) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3204) [M::mem_pestat] mean and std.dev: (1044.92, 469.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4041) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 990.998 CPU sec, 43.920 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 666690, 60, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (739, 1255, 1589) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3289) [M::mem_pestat] mean and std.dev: (1082.17, 576.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4139) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.78, 79.48) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 32, 87) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 233) [M::mem_pestat] mean and std.dev: (37.49, 36.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 306) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1115, 1322, 1853) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3329) [M::mem_pestat] mean and std.dev: (1347.60, 629.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4067) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1001.951 CPU sec, 58.634 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 666546, 73, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (263, 411, 1497) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3965) [M::mem_pestat] mean and std.dev: (741.36, 711.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5199) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.62, 79.50) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 28, 94) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 254) [M::mem_pestat] mean and std.dev: (36.97, 33.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 334) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (866, 1261, 1682) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3314) [M::mem_pestat] mean and std.dev: (1191.33, 373.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4130) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1001.054 CPU sec, 44.733 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 667312, 50, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (344, 1074, 1389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3479) [M::mem_pestat] mean and std.dev: (952.00, 549.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4524) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.67, 79.59) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 27, 133) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 369) [M::mem_pestat] mean and std.dev: (48.16, 55.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 487) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (711, 1065, 1703) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3687) [M::mem_pestat] mean and std.dev: (1140.69, 741.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4679) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 993.864 CPU sec, 56.734 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 666466, 60, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (468, 948, 2557) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6735) [M::mem_pestat] mean and std.dev: (1259.18, 1039.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8824) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.74, 79.49) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 26, 90) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 242) [M::mem_pestat] mean and std.dev: (33.39, 40.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 318) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (235, 830, 1305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3445) [M::mem_pestat] mean and std.dev: (694.25, 440.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4515) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 996.669 CPU sec, 44.221 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 666390, 69, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (234, 769, 1562) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4218) [M::mem_pestat] mean and std.dev: (827.94, 751.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5546) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.66, 79.37) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 30, 94) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 254) [M::mem_pestat] mean and std.dev: (29.46, 25.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 334) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (805, 898, 1013) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (389, 1429) [M::mem_pestat] mean and std.dev: (765.00, 213.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1637) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1000.272 CPU sec, 58.141 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 666446, 65, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (834, 1252, 2329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5319) [M::mem_pestat] mean and std.dev: (1277.00, 798.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6814) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.84, 79.61) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 32, 111) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 305) [M::mem_pestat] mean and std.dev: (39.33, 47.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 402) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (948, 1316, 4699) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12201) [M::mem_pestat] mean and std.dev: (2321.18, 2026.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15952) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 996.696 CPU sec, 44.230 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 666590, 64, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (204, 1124, 2899) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8289) [M::mem_pestat] mean and std.dev: (1246.58, 1104.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10984) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.75, 79.68) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 34, 155) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 437) [M::mem_pestat] mean and std.dev: (41.65, 58.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 997.245 CPU sec, 58.374 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 666792, 60, 12) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.83, 79.37) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 40, 103) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 273) [M::mem_pestat] mean and std.dev: (41.84, 42.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 358) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (755, 1012, 1405) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2705) [M::mem_pestat] mean and std.dev: (1128.67, 576.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3436) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 993.103 CPU sec, 44.225 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 666815, 56, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (378, 1443, 1815) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4689) [M::mem_pestat] mean and std.dev: (1320.94, 1160.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6126) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.61, 79.50) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 34, 122) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 336) [M::mem_pestat] mean and std.dev: (34.64, 35.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 443) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 997.217 CPU sec, 57.044 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 666744, 54, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (207, 319, 917) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2337) [M::mem_pestat] mean and std.dev: (475.54, 356.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3047) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.76, 79.47) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 27, 73) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 193) [M::mem_pestat] mean and std.dev: (28.35, 24.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 253) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (758, 1197, 2892) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7160) [M::mem_pestat] mean and std.dev: (1485.50, 1033.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9294) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 993.996 CPU sec, 44.201 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 666306, 67, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (403, 608, 2014) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5236) [M::mem_pestat] mean and std.dev: (1189.07, 1159.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6847) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.77, 79.63) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 40, 95) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 245) [M::mem_pestat] mean and std.dev: (36.28, 27.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 320) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 993.994 CPU sec, 51.671 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 666498, 69, 20) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.57, 79.53) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 32, 96) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 260) [M::mem_pestat] mean and std.dev: (40.59, 44.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 342) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (938, 1144, 2042) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4250) [M::mem_pestat] mean and std.dev: (1354.37, 958.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5354) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1006.485 CPU sec, 58.759 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 666495, 68, 13) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.74, 79.50) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 37, 111) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 299) [M::mem_pestat] mean and std.dev: (41.89, 46.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 393) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (543, 913, 4168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11418) [M::mem_pestat] mean and std.dev: (1746.38, 1930.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15043) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1001.900 CPU sec, 44.587 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 666651, 58, 10) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.86, 79.47) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 35, 92) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 232) [M::mem_pestat] mean and std.dev: (38.53, 32.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 302) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (677, 1600, 2894) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7328) [M::mem_pestat] mean and std.dev: (1833.00, 1406.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9545) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 986.626 CPU sec, 56.426 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 666689, 61, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (459, 1016, 2194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5664) [M::mem_pestat] mean and std.dev: (1115.24, 808.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7399) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.84, 79.52) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 33, 70) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 172) [M::mem_pestat] mean and std.dev: (38.76, 33.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 223) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1146, 1243, 2394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4890) [M::mem_pestat] mean and std.dev: (1816.64, 1196.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6603) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 999.871 CPU sec, 44.411 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 666144, 56, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (252, 337, 1605) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4311) [M::mem_pestat] mean and std.dev: (794.09, 883.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5664) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.66, 79.47) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 33, 65) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 161) [M::mem_pestat] mean and std.dev: (33.96, 25.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 209) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (736, 1407, 2542) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6154) [M::mem_pestat] mean and std.dev: (1786.70, 1624.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8285) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 996.913 CPU sec, 57.262 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 667039, 73, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (418, 456, 1074) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2386) [M::mem_pestat] mean and std.dev: (617.83, 404.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3042) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.79, 79.47) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 40, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (39.32, 45.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 788) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 999.893 CPU sec, 44.267 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 666638, 69, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (360, 689, 1780) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4620) [M::mem_pestat] mean and std.dev: (628.18, 520.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6040) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.71, 79.30) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 30, 138) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 382) [M::mem_pestat] mean and std.dev: (34.95, 37.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 504) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (898, 1159, 1715) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3349) [M::mem_pestat] mean and std.dev: (1114.00, 492.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4166) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 994.946 CPU sec, 57.772 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 667067, 65, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (293, 500, 1511) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3947) [M::mem_pestat] mean and std.dev: (879.33, 905.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5165) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.74, 79.46) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 45, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 566) [M::mem_pestat] mean and std.dev: (65.45, 94.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 747) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (859, 1738, 2206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4900) [M::mem_pestat] mean and std.dev: (1488.11, 794.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6247) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 997.607 CPU sec, 44.239 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 666933, 71, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (267, 351, 2473) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6885) [M::mem_pestat] mean and std.dev: (1176.54, 1753.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9091) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.56, 79.50) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 32, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 557) [M::mem_pestat] mean and std.dev: (51.05, 69.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 737) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (853, 1148, 1618) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3148) [M::mem_pestat] mean and std.dev: (1218.91, 737.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4169) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1000.046 CPU sec, 57.820 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 666208, 61, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (325, 548, 1396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3538) [M::mem_pestat] mean and std.dev: (811.69, 689.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4609) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.90, 79.51) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 37, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924) [M::mem_pestat] mean and std.dev: (86.46, 158.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1227) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (796, 1309, 1616) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3256) [M::mem_pestat] mean and std.dev: (1121.44, 640.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4076) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 996.883 CPU sec, 44.433 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 666388, 60, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.78, 79.32) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 33, 64) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 156) [M::mem_pestat] mean and std.dev: (30.06, 23.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 202) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1003.051 CPU sec, 58.071 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 666829, 56, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (314, 565, 846) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1910) [M::mem_pestat] mean and std.dev: (426.20, 262.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2442) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.77, 79.55) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 36, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675) [M::mem_pestat] mean and std.dev: (60.49, 97.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 893) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (375, 789, 1350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3300) [M::mem_pestat] mean and std.dev: (796.20, 679.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4275) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1003.190 CPU sec, 44.556 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 666687, 64, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (892, 1528, 1999) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4213) [M::mem_pestat] mean and std.dev: (1435.75, 813.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5320) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.84, 79.73) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 45, 561) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1619) [M::mem_pestat] mean and std.dev: (192.32, 372.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2148) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1001.437 CPU sec, 58.480 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 666226, 58, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.98, 79.38) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 28, 65) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 167) [M::mem_pestat] mean and std.dev: (34.47, 32.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 218) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1006.013 CPU sec, 44.815 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 666384, 68, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.71, 79.58) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 36, 74) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 198) [M::mem_pestat] mean and std.dev: (37.15, 30.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 260) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1002.445 CPU sec, 58.348 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 666749, 78, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (378, 609, 1535) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3849) [M::mem_pestat] mean and std.dev: (909.23, 762.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5006) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.82, 79.42) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 36, 91) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 247) [M::mem_pestat] mean and std.dev: (28.87, 25.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 325) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (774, 1456, 2294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5334) [M::mem_pestat] mean and std.dev: (1558.11, 1033.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6854) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1003.266 CPU sec, 44.702 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 666288, 61, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (269, 602, 1971) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5375) [M::mem_pestat] mean and std.dev: (890.15, 795.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7077) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.82, 79.52) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 29, 137) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 379) [M::mem_pestat] mean and std.dev: (37.45, 57.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 500) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (650, 942, 1418) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2954) [M::mem_pestat] mean and std.dev: (917.90, 492.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3722) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1009.393 CPU sec, 58.651 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 667205, 58, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (427, 768, 1405) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3361) [M::mem_pestat] mean and std.dev: (1080.07, 906.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4704) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.55, 79.65) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 52, 383) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1111) [M::mem_pestat] mean and std.dev: (84.24, 147.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1475) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 996.276 CPU sec, 44.210 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 666453, 57, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (329, 695, 1262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3128) [M::mem_pestat] mean and std.dev: (695.54, 525.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4061) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.93, 79.58) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 46, 604) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1774) [M::mem_pestat] mean and std.dev: (195.94, 374.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2359) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (894, 1718, 2280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5052) [M::mem_pestat] mean and std.dev: (1718.33, 1075.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6438) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 993.240 CPU sec, 57.205 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 667038, 63, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (489, 1454, 1906) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4740) [M::mem_pestat] mean and std.dev: (1290.47, 979.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6157) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.69, 79.36) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 41, 70) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 170) [M::mem_pestat] mean and std.dev: (34.67, 23.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 220) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (490, 848, 1231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2713) [M::mem_pestat] mean and std.dev: (810.60, 690.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3572) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 990.412 CPU sec, 43.839 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 666809, 69, 12) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.71, 79.43) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 45, 105) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 271) [M::mem_pestat] mean and std.dev: (40.55, 33.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 354) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (934, 1464, 1768) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3436) [M::mem_pestat] mean and std.dev: (1286.18, 761.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4332) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 995.953 CPU sec, 55.497 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 666180, 64, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (578, 1046, 1588) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3608) [M::mem_pestat] mean and std.dev: (1105.00, 748.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4618) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.73, 79.64) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 48, 332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944) [M::mem_pestat] mean and std.dev: (105.45, 193.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1250) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (793, 1461, 1975) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4339) [M::mem_pestat] mean and std.dev: (1492.33, 832.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5521) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1001.680 CPU sec, 57.135 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 666759, 56, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (379, 912, 1667) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4243) [M::mem_pestat] mean and std.dev: (841.17, 513.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5531) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.76, 79.50) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 32, 101) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 269) [M::mem_pestat] mean and std.dev: (34.04, 30.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 353) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (559, 1144, 1593) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3661) [M::mem_pestat] mean and std.dev: (1092.21, 603.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4695) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 997.734 CPU sec, 44.467 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 666454, 70, 16) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.63, 79.59) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 36, 69) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 177) [M::mem_pestat] mean and std.dev: (35.74, 30.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 231) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (340, 1105, 1753) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4579) [M::mem_pestat] mean and std.dev: (1103.27, 848.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5992) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1000.250 CPU sec, 57.186 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 666014, 76, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (551, 1132, 3076) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8126) [M::mem_pestat] mean and std.dev: (1465.22, 1056.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10651) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.73, 79.61) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 33, 149) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (47.97, 59.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 557) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (724, 920, 1955) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4417) [M::mem_pestat] mean and std.dev: (1053.33, 587.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5648) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 992.142 CPU sec, 44.222 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 667188, 65, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (615, 1131, 2018) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4824) [M::mem_pestat] mean and std.dev: (1229.23, 785.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6227) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.70, 79.47) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 31, 114) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 308) [M::mem_pestat] mean and std.dev: (38.43, 39.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 405) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 989.765 CPU sec, 56.122 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 666375, 63, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.65, 79.71) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 29, 61) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 155) [M::mem_pestat] mean and std.dev: (29.94, 23.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 202) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 998.781 CPU sec, 44.442 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 666618, 79, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (246, 385, 783) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1857) [M::mem_pestat] mean and std.dev: (451.56, 314.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2394) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.76, 79.49) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 40, 109) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 295) [M::mem_pestat] mean and std.dev: (43.78, 48.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 388) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (939, 1513, 2949) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6969) [M::mem_pestat] mean and std.dev: (1612.44, 1119.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8979) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 992.364 CPU sec, 57.975 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 666606, 56, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (281, 623, 1602) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4244) [M::mem_pestat] mean and std.dev: (857.12, 806.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5565) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.78, 79.42) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 37, 59) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 145) [M::mem_pestat] mean and std.dev: (32.15, 24.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 188) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (434, 1156, 1857) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4703) [M::mem_pestat] mean and std.dev: (1074.36, 684.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6126) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 998.721 CPU sec, 44.406 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 666752, 53, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (668, 908, 1777) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3995) [M::mem_pestat] mean and std.dev: (1131.84, 827.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5104) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.63, 79.68) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 41, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 489) [M::mem_pestat] mean and std.dev: (40.40, 39.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 645) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 994.203 CPU sec, 56.818 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 666226, 57, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (547, 1187, 1654) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3868) [M::mem_pestat] mean and std.dev: (1086.31, 607.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4975) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.71, 79.54) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 42, 159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (54.40, 67.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 552) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1002.909 CPU sec, 44.828 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 666738, 63, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (209, 416, 1481) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4025) [M::mem_pestat] mean and std.dev: (938.21, 1133.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5474) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.64, 79.52) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 41, 146) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 392) [M::mem_pestat] mean and std.dev: (61.07, 72.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 515) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1053, 1629, 2095) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4179) [M::mem_pestat] mean and std.dev: (1307.88, 569.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5221) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 987.253 CPU sec, 47.396 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 666415, 59, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (293, 364, 475) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 839) [M::mem_pestat] mean and std.dev: (327.18, 85.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1021) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.59, 79.56) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 32, 67) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 161) [M::mem_pestat] mean and std.dev: (33.18, 25.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 208) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1246, 1406, 2946) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6346) [M::mem_pestat] mean and std.dev: (1849.64, 1395.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8046) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 997.252 CPU sec, 57.218 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 666856, 56, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (678, 1082, 1499) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3141) [M::mem_pestat] mean and std.dev: (1043.60, 639.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3962) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.78, 79.40) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 48, 492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1434) [M::mem_pestat] mean and std.dev: (110.35, 255.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1905) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (647, 1326, 3103) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8015) [M::mem_pestat] mean and std.dev: (1943.00, 1834.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10471) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 993.333 CPU sec, 44.011 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 666662, 79, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (136, 703, 1083) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2977) [M::mem_pestat] mean and std.dev: (595.50, 465.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3924) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.87, 79.46) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 37, 124) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 342) [M::mem_pestat] mean and std.dev: (42.97, 46.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 451) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1212, 1474, 2031) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3669) [M::mem_pestat] mean and std.dev: (1547.25, 557.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4488) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 991.951 CPU sec, 56.119 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 666914, 65, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (150, 329, 993) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2679) [M::mem_pestat] mean and std.dev: (592.15, 691.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3522) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.78, 79.51) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 31, 119) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 319) [M::mem_pestat] mean and std.dev: (34.31, 36.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 419) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 986.507 CPU sec, 43.739 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 666579, 65, 11) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.60, 79.63) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 42, 117) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 303) [M::mem_pestat] mean and std.dev: (40.85, 34.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 396) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1089, 1361, 1751) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3075) [M::mem_pestat] mean and std.dev: (1185.20, 657.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3814) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 990.155 CPU sec, 56.758 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 666258, 68, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (440, 1029, 1623) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3989) [M::mem_pestat] mean and std.dev: (1020.31, 733.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5172) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.71, 79.67) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 29, 107) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 297) [M::mem_pestat] mean and std.dev: (39.41, 51.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 392) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (729, 1510, 2545) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6177) [M::mem_pestat] mean and std.dev: (1544.14, 1155.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7993) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 997.147 CPU sec, 44.341 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 666548, 56, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (328, 563, 2544) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6976) [M::mem_pestat] mean and std.dev: (1275.85, 1305.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9192) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.70, 79.46) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 34, 105) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 285) [M::mem_pestat] mean and std.dev: (39.20, 47.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 375) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 991.373 CPU sec, 57.039 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 666120, 71, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (261, 390, 1407) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3699) [M::mem_pestat] mean and std.dev: (740.36, 996.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4845) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.61, 79.38) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 27, 82) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 214) [M::mem_pestat] mean and std.dev: (36.08, 41.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 280) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (509, 1059, 1775) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4307) [M::mem_pestat] mean and std.dev: (1197.41, 870.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5573) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 999.131 CPU sec, 44.401 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 667016, 66, 12) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.69, 79.46) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (10, 34, 87) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 241) [M::mem_pestat] mean and std.dev: (42.83, 51.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 318) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (634, 982, 1392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2908) [M::mem_pestat] mean and std.dev: (893.82, 368.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3666) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 992.041 CPU sec, 57.093 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666621, 54, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1133, 1885, 2425) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5009) [M::mem_pestat] mean and std.dev: (1654.18, 1055.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6301) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.76, 79.59) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 53, 1559) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4637) [M::mem_pestat] mean and std.dev: (815.51, 1338.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6176) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 999.947 CPU sec, 44.475 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 666912, 75, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (622, 1548, 1793) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4135) [M::mem_pestat] mean and std.dev: (1226.62, 651.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5306) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.73, 79.50) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 28, 154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 434) [M::mem_pestat] mean and std.dev: (35.10, 45.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 574) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 998.273 CPU sec, 57.153 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 667303, 58, 11) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.69, 79.52) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 35, 68) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 176) [M::mem_pestat] mean and std.dev: (33.58, 29.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 230) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (654, 1149, 1622) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3558) [M::mem_pestat] mean and std.dev: (1137.36, 767.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4526) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 986.583 CPU sec, 43.715 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 666752, 49, 12) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.71, 79.45) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 38, 78) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 194) [M::mem_pestat] mean and std.dev: (35.62, 32.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 252) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (925, 1387, 2850) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6700) [M::mem_pestat] mean and std.dev: (1277.36, 977.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8625) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1001.822 CPU sec, 58.510 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 667144, 49, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (361, 1035, 2559) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6955) [M::mem_pestat] mean and std.dev: (1547.67, 1644.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9153) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.75, 79.44) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 35, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758) [M::mem_pestat] mean and std.dev: (56.15, 119.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1006) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (605, 976, 1097) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2081) [M::mem_pestat] mean and std.dev: (801.45, 332.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2573) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 989.853 CPU sec, 43.946 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 666283, 64, 13) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.50, 79.42) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 34, 125) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 339) [M::mem_pestat] mean and std.dev: (48.61, 57.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 446) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (639, 1009, 2058) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4896) [M::mem_pestat] mean and std.dev: (1412.23, 1114.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6315) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 997.960 CPU sec, 57.783 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 667128, 54, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (244, 653, 1415) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3757) [M::mem_pestat] mean and std.dev: (811.14, 652.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4928) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.74, 79.62) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 54, 819) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2419) [M::mem_pestat] mean and std.dev: (231.47, 543.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3219) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 999.624 CPU sec, 44.233 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 666632, 54, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (235, 508, 1410) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3760) [M::mem_pestat] mean and std.dev: (734.38, 718.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4935) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.69, 79.51) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (10, 33, 116) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 328) [M::mem_pestat] mean and std.dev: (45.51, 59.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 434) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (680, 976, 1592) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3416) [M::mem_pestat] mean and std.dev: (991.31, 482.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4328) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 993.492 CPU sec, 56.757 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 667115, 69, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (277, 1207, 2628) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7330) [M::mem_pestat] mean and std.dev: (1210.07, 1080.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9681) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.86, 79.48) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 27, 93) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 251) [M::mem_pestat] mean and std.dev: (31.59, 34.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 330) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1278, 1455, 3002) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6450) [M::mem_pestat] mean and std.dev: (1643.70, 1009.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8174) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 995.533 CPU sec, 44.135 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 666363, 59, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (432, 984, 1875) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4761) [M::mem_pestat] mean and std.dev: (1002.57, 795.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6204) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.61, 79.59) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 43, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 514) [M::mem_pestat] mean and std.dev: (54.40, 63.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 678) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 997.316 CPU sec, 57.013 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 666758, 64, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (174, 720, 979) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2589) [M::mem_pestat] mean and std.dev: (683.71, 554.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3394) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.78, 79.47) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (10, 32, 62) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 166) [M::mem_pestat] mean and std.dev: (30.16, 27.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 218) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 996.500 CPU sec, 44.248 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 667355, 58, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (271, 474, 1516) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4006) [M::mem_pestat] mean and std.dev: (829.50, 708.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5251) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.76, 79.47) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 42, 84) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 204) [M::mem_pestat] mean and std.dev: (41.15, 29.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 264) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1063, 1447, 1932) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3670) [M::mem_pestat] mean and std.dev: (1261.62, 612.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4539) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 996.503 CPU sec, 52.338 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 666286, 75, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (258, 530, 1026) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2562) [M::mem_pestat] mean and std.dev: (720.82, 619.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3330) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.79, 79.59) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 47, 149) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (57.08, 73.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 539) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (729, 1216, 1539) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3159) [M::mem_pestat] mean and std.dev: (1095.75, 447.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3969) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1010.092 CPU sec, 58.990 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 666455, 69, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (353, 757, 1242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3020) [M::mem_pestat] mean and std.dev: (744.88, 521.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3909) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.78, 79.54) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 38, 109) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 293) [M::mem_pestat] mean and std.dev: (45.83, 51.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 385) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 996.711 CPU sec, 44.907 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 666578, 79, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (322, 914, 1572) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4072) [M::mem_pestat] mean and std.dev: (933.33, 625.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5322) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.71, 79.66) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 35, 85) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 221) [M::mem_pestat] mean and std.dev: (33.78, 28.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 289) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 993.629 CPU sec, 57.449 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 666725, 49, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (294, 688, 1526) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3990) [M::mem_pestat] mean and std.dev: (770.56, 602.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5222) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.62, 79.74) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 32, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 574) [M::mem_pestat] mean and std.dev: (39.51, 56.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 760) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (937, 1216, 1686) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3184) [M::mem_pestat] mean and std.dev: (1251.22, 639.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3933) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 995.124 CPU sec, 44.171 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 666965, 57, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (236, 562, 1474) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3950) [M::mem_pestat] mean and std.dev: (587.86, 503.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5188) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.69, 79.44) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 27, 54) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 138) [M::mem_pestat] mean and std.dev: (29.04, 27.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 180) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (786, 1530, 1930) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4218) [M::mem_pestat] mean and std.dev: (1265.94, 747.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5362) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1002.179 CPU sec, 57.942 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 666817, 67, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (789, 1228, 2501) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5925) [M::mem_pestat] mean and std.dev: (1463.00, 1126.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7637) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.71, 79.50) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 33, 65) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 167) [M::mem_pestat] mean and std.dev: (31.16, 25.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 218) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 990.679 CPU sec, 44.023 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 667404, 50, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (412, 905, 2064) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5368) [M::mem_pestat] mean and std.dev: (1228.12, 1167.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7020) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.69, 79.52) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 39, 140) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 370) [M::mem_pestat] mean and std.dev: (45.55, 42.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 485) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 990.689 CPU sec, 55.881 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 667047, 62, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (269, 957, 1211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3095) [M::mem_pestat] mean and std.dev: (847.45, 752.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4037) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.68, 79.46) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 37, 101) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (44.17, 47.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 365) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (311, 871, 1590) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4148) [M::mem_pestat] mean and std.dev: (1106.69, 911.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5427) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 993.977 CPU sec, 44.023 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 666253, 71, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (794, 1298, 1494) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2894) [M::mem_pestat] mean and std.dev: (1029.09, 423.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3594) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.69, 79.68) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 37, 88) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 240) [M::mem_pestat] mean and std.dev: (30.72, 27.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 316) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1025, 1660, 2311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4883) [M::mem_pestat] mean and std.dev: (1509.88, 870.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6169) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1003.507 CPU sec, 58.059 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 666457, 55, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (287, 1201, 1551) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4079) [M::mem_pestat] mean and std.dev: (953.65, 801.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5343) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.75, 79.65) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 38, 98) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 262) [M::mem_pestat] mean and std.dev: (33.84, 26.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 344) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (757, 1372, 2644) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6418) [M::mem_pestat] mean and std.dev: (1297.70, 938.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8305) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 998.143 CPU sec, 44.416 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 666943, 65, 12) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.64, 79.48) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 29, 70) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 182) [M::mem_pestat] mean and std.dev: (32.13, 31.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 238) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1211, 1640, 2342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4604) [M::mem_pestat] mean and std.dev: (1335.20, 614.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5735) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 986.770 CPU sec, 54.832 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 666503, 61, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (357, 1085, 1326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3264) [M::mem_pestat] mean and std.dev: (932.46, 550.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4233) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.73, 79.55) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 37, 93) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 239) [M::mem_pestat] mean and std.dev: (36.71, 27.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 312) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (139, 1531, 2103) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6031) [M::mem_pestat] mean and std.dev: (1198.70, 866.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7995) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 991.015 CPU sec, 44.041 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 666729, 63, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (281, 990, 2275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6263) [M::mem_pestat] mean and std.dev: (1182.08, 1165.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8257) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.89, 79.42) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 31, 74) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 192) [M::mem_pestat] mean and std.dev: (37.62, 35.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 251) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 994.056 CPU sec, 54.641 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 666248, 60, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (538, 1301, 2589) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6691) [M::mem_pestat] mean and std.dev: (1664.11, 1422.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8742) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.77, 79.48) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 41, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (36.98, 43.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 624) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 991.020 CPU sec, 43.932 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 665904, 60, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (297, 425, 921) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2169) [M::mem_pestat] mean and std.dev: (536.53, 399.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2793) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.60, 79.36) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 40, 64) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 156) [M::mem_pestat] mean and std.dev: (32.10, 24.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 202) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1000.775 CPU sec, 57.268 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 666592, 56, 12) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.72, 79.36) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 25, 66) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 174) [M::mem_pestat] mean and std.dev: (28.77, 23.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 228) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (791, 1293, 3543) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9047) [M::mem_pestat] mean and std.dev: (2469.25, 2503.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12482) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 990.649 CPU sec, 43.966 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 666423, 66, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (333, 496, 1744) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4566) [M::mem_pestat] mean and std.dev: (1055.38, 1078.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5977) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.78, 79.56) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 34, 106) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 288) [M::mem_pestat] mean and std.dev: (39.80, 40.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 379) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (541, 1031, 1419) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3175) [M::mem_pestat] mean and std.dev: (868.40, 488.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4053) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 988.413 CPU sec, 55.843 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 666557, 53, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (470, 813, 1286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2918) [M::mem_pestat] mean and std.dev: (706.00, 412.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3734) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.67, 79.61) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 37, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (48.12, 65.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 672) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 996.270 CPU sec, 44.335 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 666361, 62, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (355, 1275, 2110) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5620) [M::mem_pestat] mean and std.dev: (1386.90, 1025.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7375) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.65, 79.56) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 55, 1688) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5028) [M::mem_pestat] mean and std.dev: (995.61, 1588.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7351) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (994, 1535, 2582) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5758) [M::mem_pestat] mean and std.dev: (1782.18, 1167.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7346) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 996.303 CPU sec, 56.785 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 666834, 52, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (261, 339, 1030) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2568) [M::mem_pestat] mean and std.dev: (596.85, 473.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3337) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.87, 79.51) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 40, 570) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1668) [M::mem_pestat] mean and std.dev: (105.58, 215.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2217) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1170, 2006, 3159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7137) [M::mem_pestat] mean and std.dev: (1879.40, 1242.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9126) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 991.268 CPU sec, 44.072 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 666255, 66, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (276, 362, 992) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2424) [M::mem_pestat] mean and std.dev: (555.20, 453.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3140) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.84, 79.58) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 38, 129) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 347) [M::mem_pestat] mean and std.dev: (58.09, 68.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 456) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1066, 2317, 3690) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8938) [M::mem_pestat] mean and std.dev: (2446.17, 1997.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11562) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1001.950 CPU sec, 54.422 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 667067, 64, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (348, 597, 1355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3369) [M::mem_pestat] mean and std.dev: (633.20, 403.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4376) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.75, 79.53) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 38, 60) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 148) [M::mem_pestat] mean and std.dev: (32.94, 23.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 192) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 992.767 CPU sec, 56.818 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 667065, 53, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (298, 748, 1944) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5236) [M::mem_pestat] mean and std.dev: (1003.78, 873.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6882) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.72, 79.57) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 41, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627) [M::mem_pestat] mean and std.dev: (72.91, 108.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 829) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 995.247 CPU sec, 44.343 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 667150, 52, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (406, 695, 1225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2863) [M::mem_pestat] mean and std.dev: (717.07, 441.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3682) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.90, 79.53) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 46, 113) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 297) [M::mem_pestat] mean and std.dev: (47.16, 41.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 389) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (638, 1180, 1272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2540) [M::mem_pestat] mean and std.dev: (966.00, 498.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3174) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 985.455 CPU sec, 55.367 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 666290, 56, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (365, 822, 1230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2960) [M::mem_pestat] mean and std.dev: (841.69, 612.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3825) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.79, 79.53) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 39, 123) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 333) [M::mem_pestat] mean and std.dev: (45.37, 55.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 438) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (433, 1310, 2135) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5539) [M::mem_pestat] mean and std.dev: (1327.27, 1138.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7241) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 995.669 CPU sec, 44.358 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 667229, 62, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (351, 966, 2908) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8022) [M::mem_pestat] mean and std.dev: (1406.35, 1191.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10579) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.78, 79.49) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 36, 105) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 281) [M::mem_pestat] mean and std.dev: (37.58, 38.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 369) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1228, 1390, 2362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4630) [M::mem_pestat] mean and std.dev: (1756.25, 1030.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5877) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 982.125 CPU sec, 55.846 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 666749, 60, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (264, 449, 863) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2061) [M::mem_pestat] mean and std.dev: (593.54, 486.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2660) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.77, 79.53) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 39, 147) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (44.73, 49.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 534) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (296, 1320, 1984) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5360) [M::mem_pestat] mean and std.dev: (1336.00, 1061.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7048) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 989.023 CPU sec, 43.957 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 666485, 56, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (262, 684, 1169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2983) [M::mem_pestat] mean and std.dev: (673.88, 454.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3890) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.77, 79.53) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 35, 117) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 323) [M::mem_pestat] mean and std.dev: (44.94, 57.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 426) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (932, 1533, 2875) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6761) [M::mem_pestat] mean and std.dev: (1745.07, 1398.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8704) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 987.032 CPU sec, 55.243 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 666646, 58, 10) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.74, 79.55) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 47, 1278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3784) [M::mem_pestat] mean and std.dev: (531.49, 947.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5037) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (677, 1444, 2050) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4796) [M::mem_pestat] mean and std.dev: (1138.78, 697.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6169) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 989.601 CPU sec, 43.820 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 666247, 68, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (561, 1300, 1463) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3267) [M::mem_pestat] mean and std.dev: (892.10, 454.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4169) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.74, 79.57) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 34, 123) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 345) [M::mem_pestat] mean and std.dev: (35.30, 43.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 456) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (713, 935, 2415) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5819) [M::mem_pestat] mean and std.dev: (1588.58, 1389.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7521) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 990.027 CPU sec, 56.308 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 666235, 67, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (334, 828, 2307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6253) [M::mem_pestat] mean and std.dev: (1213.24, 1055.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8226) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.72, 79.43) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 39, 78) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 198) [M::mem_pestat] mean and std.dev: (36.11, 27.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 258) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (343, 1171, 1787) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4675) [M::mem_pestat] mean and std.dev: (1251.30, 868.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6119) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 989.708 CPU sec, 43.903 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 666742, 67, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (238, 289, 655) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1489) [M::mem_pestat] mean and std.dev: (401.67, 247.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1906) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.68, 79.47) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 33, 134) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 370) [M::mem_pestat] mean and std.dev: (36.42, 37.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 488) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (713, 1066, 2035) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4679) [M::mem_pestat] mean and std.dev: (1374.62, 800.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6001) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 985.147 CPU sec, 46.296 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 666408, 63, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (257, 509, 1447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3827) [M::mem_pestat] mean and std.dev: (863.00, 783.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5017) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.84, 79.44) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 36, 68) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 158) [M::mem_pestat] mean and std.dev: (33.38, 22.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 203) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 997.706 CPU sec, 57.010 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 666359, 79, 12) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.88, 79.58) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 34, 105) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 287) [M::mem_pestat] mean and std.dev: (40.84, 49.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 378) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (482, 1207, 2179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5573) [M::mem_pestat] mean and std.dev: (1404.91, 1314.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7270) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 992.116 CPU sec, 44.074 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666267, 84, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (326, 715, 1310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3278) [M::mem_pestat] mean and std.dev: (521.56, 373.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4262) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.74, 79.47) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 31, 82) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 218) [M::mem_pestat] mean and std.dev: (32.94, 26.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 286) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1020, 1476, 1883) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3609) [M::mem_pestat] mean and std.dev: (1534.89, 669.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4472) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 989.102 CPU sec, 55.210 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 666451, 55, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (338, 542, 1426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3602) [M::mem_pestat] mean and std.dev: (732.82, 630.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4690) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.83, 79.52) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 35, 77) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 197) [M::mem_pestat] mean and std.dev: (33.31, 34.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 257) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (327, 972, 2651) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7299) [M::mem_pestat] mean and std.dev: (1390.10, 1197.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9623) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 991.329 CPU sec, 43.872 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 666354, 65, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (438, 859, 1943) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4953) [M::mem_pestat] mean and std.dev: (1089.69, 780.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6458) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.85, 79.54) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 25, 66) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 172) [M::mem_pestat] mean and std.dev: (28.21, 27.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 225) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 987.454 CPU sec, 55.659 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 666480, 65, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (167, 375, 1178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3200) [M::mem_pestat] mean and std.dev: (639.76, 591.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4211) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.67, 79.55) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 38, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (55.84, 71.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 664) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (909, 1181, 2513) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5721) [M::mem_pestat] mean and std.dev: (1279.46, 767.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7325) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 988.831 CPU sec, 43.896 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 666799, 70, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (358, 964, 2672) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7300) [M::mem_pestat] mean and std.dev: (1124.75, 1019.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9614) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.77, 79.48) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 36, 128) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 350) [M::mem_pestat] mean and std.dev: (39.80, 51.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (834, 1305, 3091) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7605) [M::mem_pestat] mean and std.dev: (1639.00, 1180.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9862) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 989.742 CPU sec, 55.763 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 666705, 70, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (292, 571, 1460) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3796) [M::mem_pestat] mean and std.dev: (831.83, 637.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4964) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.58, 79.65) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 37, 348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1012) [M::mem_pestat] mean and std.dev: (58.13, 112.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1344) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1397, 1562, 4133) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9605) [M::mem_pestat] mean and std.dev: (2527.50, 2361.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12341) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 988.831 CPU sec, 43.710 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 666944, 67, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (262, 870, 1194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3058) [M::mem_pestat] mean and std.dev: (817.14, 501.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3990) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.73, 79.73) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 41, 111) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 307) [M::mem_pestat] mean and std.dev: (38.23, 40.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 405) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 986.813 CPU sec, 56.187 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666854, 79, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (420, 547, 1898) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4854) [M::mem_pestat] mean and std.dev: (1318.55, 1520.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7401) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.88, 79.49) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 28, 107) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 289) [M::mem_pestat] mean and std.dev: (35.40, 35.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 380) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (892, 2193, 2821) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6679) [M::mem_pestat] mean and std.dev: (2036.00, 1437.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8608) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 992.950 CPU sec, 44.067 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 666968, 58, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (241, 392, 1338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3532) [M::mem_pestat] mean and std.dev: (675.75, 718.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4629) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.68, 79.60) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 37, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 459) [M::mem_pestat] mean and std.dev: (55.19, 81.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 608) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 990.403 CPU sec, 56.000 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666466, 54, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (405, 567, 1823) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4659) [M::mem_pestat] mean and std.dev: (875.30, 686.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6077) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.70, 79.65) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 51, 456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1330) [M::mem_pestat] mean and std.dev: (76.43, 158.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1767) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (748, 1296, 1556) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3172) [M::mem_pestat] mean and std.dev: (1136.25, 779.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4255) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 994.455 CPU sec, 44.266 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666473, 65, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (781, 1290, 2083) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4687) [M::mem_pestat] mean and std.dev: (1299.00, 864.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5989) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.65, 79.53) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 32, 109) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 297) [M::mem_pestat] mean and std.dev: (34.13, 37.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 391) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (937, 1253, 1489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2593) [M::mem_pestat] mean and std.dev: (1204.80, 454.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3145) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 983.175 CPU sec, 53.770 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 666199, 65, 12) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.69, 79.41) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 34, 72) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 182) [M::mem_pestat] mean and std.dev: (35.84, 26.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 237) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (654, 1101, 2340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5712) [M::mem_pestat] mean and std.dev: (1473.08, 1151.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7398) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 994.405 CPU sec, 44.035 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 666542, 65, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (358, 774, 1195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2869) [M::mem_pestat] mean and std.dev: (903.00, 810.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4146) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.88, 79.46) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 37, 140) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 390) [M::mem_pestat] mean and std.dev: (41.94, 53.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 515) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (890, 1304, 1614) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3062) [M::mem_pestat] mean and std.dev: (1231.67, 527.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3786) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 987.206 CPU sec, 54.158 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 666322, 59, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (174, 834, 1968) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5556) [M::mem_pestat] mean and std.dev: (1035.43, 838.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7350) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.75, 79.60) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 39, 144) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 398) [M::mem_pestat] mean and std.dev: (44.52, 55.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 525) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (793, 948, 2043) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4543) [M::mem_pestat] mean and std.dev: (1200.91, 829.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5793) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 988.712 CPU sec, 43.978 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 666571, 72, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (291, 966, 1827) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4899) [M::mem_pestat] mean and std.dev: (931.58, 763.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6435) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.61, 79.53) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 32, 91) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 247) [M::mem_pestat] mean and std.dev: (34.53, 34.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 325) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (245, 346, 1256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3278) [M::mem_pestat] mean and std.dev: (684.08, 582.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4289) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 992.710 CPU sec, 54.512 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 666987, 67, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (226, 311, 1067) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2749) [M::mem_pestat] mean and std.dev: (491.94, 417.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3590) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.75, 79.35) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 38, 99) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 257) [M::mem_pestat] mean and std.dev: (45.50, 48.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 336) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (552, 1281, 2518) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6450) [M::mem_pestat] mean and std.dev: (1665.69, 1329.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8416) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 990.192 CPU sec, 43.986 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 666759, 57, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (487, 1177, 3645) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9961) [M::mem_pestat] mean and std.dev: (1590.69, 1332.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13119) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.68, 79.67) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 35, 150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (44.53, 58.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 537) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (736, 1075, 1452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2884) [M::mem_pestat] mean and std.dev: (985.24, 615.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3600) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 986.521 CPU sec, 56.655 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 666912, 65, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (360, 1041, 1535) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3885) [M::mem_pestat] mean and std.dev: (1170.00, 1017.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5240) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.70, 79.59) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (11, 27, 111) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 311) [M::mem_pestat] mean and std.dev: (37.36, 45.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 411) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 995.659 CPU sec, 44.171 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 526270 sequences (78940500 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 666706, 71, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (263, 593, 1049) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2621) [M::mem_pestat] mean and std.dev: (575.38, 423.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3407) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (332, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 650) [M::mem_pestat] mean and std.dev: (386.52, 79.57) [M::mem_pestat] low and high boundaries for proper pairs: (14, 756) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 43, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492) [M::mem_pestat] mean and std.dev: (53.00, 54.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 648) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (831, 1504, 3084) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7590) [M::mem_pestat] mean and std.dev: (1749.75, 1140.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9843) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 984.654 CPU sec, 46.842 real sec [M::process] 0 single-end sequences; 526270 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 219247, 23, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (333, 382, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 648) [M::mem_pestat] mean and std.dev: (386.52, 79.34) [M::mem_pestat] low and high boundaries for proper pairs: (18, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 34, 55) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 139) [M::mem_pestat] mean and std.dev: (25.22, 16.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 181) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 526270 reads in 327.276 CPU sec, 14.376 real sec [main] Version: 0.7.17-r1188 [main] CMD: /home/csyk2proj/tools/bwa.kit/bwa mem -p -t24 -R@RG\tID:KPGP-00357\tPL:ILLUMINA\tSM:KPGP-00357 -C /home/csyk2proj/data/bwa.kit/hs38DH.fa - [main] Real time: 42097.716 sec; CPU: 489741.257 sec