[M::bwa_idx_load_from_disk] read 3171 ALT contigs [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 665961, 32, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (323, 664, 1252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3110) [M::mem_pestat] mean and std.dev: (821.69, 732.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4039) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.20, 90.01) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 52, 1787) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5317) [M::mem_pestat] mean and std.dev: (1030.48, 1588.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7385) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (663, 1076, 2258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5448) [M::mem_pestat] mean and std.dev: (1725.90, 1628.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8240) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 978.122 CPU sec, 56.102 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 667446, 37, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (448, 949, 2392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6280) [M::mem_pestat] mean and std.dev: (1204.33, 1131.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8224) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.11, 89.91) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (9, 37, 1971) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5895) [M::mem_pestat] mean and std.dev: (785.71, 1308.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7857) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 957.829 CPU sec, 42.526 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666740, 41, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (555, 872, 1828) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4374) [M::mem_pestat] mean and std.dev: (996.70, 692.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5647) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.37, 90.23) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 179, 1865) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5533) [M::mem_pestat] mean and std.dev: (968.80, 1341.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7367) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (751, 1222, 4416) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11746) [M::mem_pestat] mean and std.dev: (2388.00, 2481.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15411) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 969.997 CPU sec, 56.062 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 667128, 47, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (372, 1349, 2082) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5502) [M::mem_pestat] mean and std.dev: (1311.77, 1098.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7212) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.20, 89.94) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 102, 2522) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7524) [M::mem_pestat] mean and std.dev: (1234.50, 1871.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10025) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 963.138 CPU sec, 42.736 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 666848, 41, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (415, 857, 1384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3322) [M::mem_pestat] mean and std.dev: (973.27, 703.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4291) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.04, 89.89) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 46, 4329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12945) [M::mem_pestat] mean and std.dev: (1603.98, 2445.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17253) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 962.473 CPU sec, 56.564 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 666762, 43, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (431, 1314, 1937) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4949) [M::mem_pestat] mean and std.dev: (978.91, 668.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6455) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.22, 90.20) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 699, 3156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9416) [M::mem_pestat] mean and std.dev: (2012.30, 2778.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13128) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (231, 1467, 1911) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5271) [M::mem_pestat] mean and std.dev: (970.33, 816.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6951) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 958.310 CPU sec, 42.584 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 666275, 30, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (386, 557, 1321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3191) [M::mem_pestat] mean and std.dev: (738.67, 496.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4126) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.37, 89.97) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 118, 2542) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7566) [M::mem_pestat] mean and std.dev: (972.63, 1536.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10078) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (605, 935, 1764) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4082) [M::mem_pestat] mean and std.dev: (869.00, 396.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5241) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 969.570 CPU sec, 56.878 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 666893, 35, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (339, 808, 2660) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7302) [M::mem_pestat] mean and std.dev: (1326.19, 1344.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9623) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (370, 426, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (130, 730) [M::mem_pestat] mean and std.dev: (431.92, 90.02) [M::mem_pestat] low and high boundaries for proper pairs: (10, 850) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 79, 2815) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8403) [M::mem_pestat] mean and std.dev: (1456.17, 1978.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11197) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1149, 1389, 1677) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (93, 2733) [M::mem_pestat] mean and std.dev: (1163.33, 449.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3261) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 961.580 CPU sec, 42.766 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 666993, 30, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (272, 1222, 2223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6125) [M::mem_pestat] mean and std.dev: (1336.46, 1261.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8076) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.23, 90.18) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (10, 39, 3256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9748) [M::mem_pestat] mean and std.dev: (2190.37, 3150.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14792) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 957.384 CPU sec, 54.574 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 667266, 39, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (423, 1130, 2218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5808) [M::mem_pestat] mean and std.dev: (1231.83, 1090.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7603) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.15, 89.98) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 457, 1848) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5510) [M::mem_pestat] mean and std.dev: (962.39, 1291.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7341) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 961.426 CPU sec, 42.813 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 666991, 43, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (312, 395, 801) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1779) [M::mem_pestat] mean and std.dev: (482.19, 256.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2268) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (370, 426, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (130, 730) [M::mem_pestat] mean and std.dev: (431.89, 90.06) [M::mem_pestat] low and high boundaries for proper pairs: (10, 850) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 41, 2152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6426) [M::mem_pestat] mean and std.dev: (748.47, 1307.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8563) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (432, 1066, 4631) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13029) [M::mem_pestat] mean and std.dev: (2692.36, 2907.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17228) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 966.060 CPU sec, 55.630 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 666407, 38, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (250, 291, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (18, 598) [M::mem_pestat] mean and std.dev: (251.91, 79.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 714) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.26, 90.08) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 38, 1241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3679) [M::mem_pestat] mean and std.dev: (472.88, 896.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4898) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (742, 1175, 1608) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3340) [M::mem_pestat] mean and std.dev: (1153.07, 697.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4206) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 965.604 CPU sec, 55.966 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 666942, 41, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (352, 454, 1319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3253) [M::mem_pestat] mean and std.dev: (602.05, 432.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4220) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.38, 90.01) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (54, 295, 3550) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10542) [M::mem_pestat] mean and std.dev: (1785.37, 2446.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14038) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1002, 1574, 1987) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3957) [M::mem_pestat] mean and std.dev: (1184.62, 561.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4942) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 961.233 CPU sec, 42.703 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 667513, 39, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (330, 437, 1925) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5115) [M::mem_pestat] mean and std.dev: (1095.33, 1001.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6710) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.43, 90.16) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 37, 355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1041) [M::mem_pestat] mean and std.dev: (111.91, 237.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1384) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (685, 872, 984) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (87, 1582) [M::mem_pestat] mean and std.dev: (863.50, 272.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1954) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 958.073 CPU sec, 55.473 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 667500, 47, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (286, 597, 1451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3781) [M::mem_pestat] mean and std.dev: (818.54, 686.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4946) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.22, 90.03) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 85, 1995) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5941) [M::mem_pestat] mean and std.dev: (969.00, 1528.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7914) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (357, 1219, 1465) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3681) [M::mem_pestat] mean and std.dev: (897.67, 541.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4789) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 959.476 CPU sec, 42.849 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 666518, 31, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (424, 1159, 2260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5932) [M::mem_pestat] mean and std.dev: (1402.00, 1343.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7768) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.12, 89.95) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 49, 1948) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5792) [M::mem_pestat] mean and std.dev: (702.04, 1162.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7714) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (350, 872, 1410) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3530) [M::mem_pestat] mean and std.dev: (914.30, 596.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4590) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 960.884 CPU sec, 54.862 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 666719, 41, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (375, 518, 1411) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3483) [M::mem_pestat] mean and std.dev: (607.13, 474.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4519) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.25, 89.97) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 50, 1725) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5141) [M::mem_pestat] mean and std.dev: (737.74, 1338.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6849) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1246, 1790, 3623) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8377) [M::mem_pestat] mean and std.dev: (2309.55, 1622.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10754) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 952.033 CPU sec, 42.207 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 666558, 37, 14) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.31, 89.98) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (39, 105, 1941) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5745) [M::mem_pestat] mean and std.dev: (941.74, 1415.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7647) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (269, 958, 1537) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4073) [M::mem_pestat] mean and std.dev: (1072.38, 975.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5341) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 947.979 CPU sec, 53.500 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 666764, 33, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (331, 666, 2663) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7327) [M::mem_pestat] mean and std.dev: (1335.62, 1690.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9659) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (431.99, 89.85) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 80, 994) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2938) [M::mem_pestat] mean and std.dev: (324.68, 624.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3910) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (756, 1179, 1826) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3966) [M::mem_pestat] mean and std.dev: (1107.44, 544.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5036) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 946.798 CPU sec, 41.996 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 666201, 37, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (436, 541, 1946) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4966) [M::mem_pestat] mean and std.dev: (1236.73, 1167.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6476) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.21, 89.99) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (45, 384, 2669) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7917) [M::mem_pestat] mean and std.dev: (1254.94, 1753.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10541) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1097, 1664, 2643) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5735) [M::mem_pestat] mean and std.dev: (1602.64, 1090.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7281) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 949.054 CPU sec, 51.360 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 667454, 37, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (922, 1099, 1326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (114, 2134) [M::mem_pestat] mean and std.dev: (939.70, 279.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2538) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.00, 89.92) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 44, 2597) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7751) [M::mem_pestat] mean and std.dev: (1114.67, 1687.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10328) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (809, 1535, 3549) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9029) [M::mem_pestat] mean and std.dev: (2127.69, 2025.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11769) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 946.450 CPU sec, 41.905 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 666799, 39, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (408, 828, 1795) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4569) [M::mem_pestat] mean and std.dev: (1055.75, 872.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5956) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.07, 89.92) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 40, 1789) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5329) [M::mem_pestat] mean and std.dev: (791.76, 1337.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7099) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 947.893 CPU sec, 52.603 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 666822, 30, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (391, 524, 1157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2689) [M::mem_pestat] mean and std.dev: (694.29, 535.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3455) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.23, 90.10) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (47, 851, 3098) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9200) [M::mem_pestat] mean and std.dev: (1934.93, 2505.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12251) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1216, 2016, 2238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4282) [M::mem_pestat] mean and std.dev: (1782.44, 992.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5754) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 948.057 CPU sec, 41.970 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 666685, 32, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (285, 723, 940) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2250) [M::mem_pestat] mean and std.dev: (644.50, 401.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2905) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (370, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (130, 730) [M::mem_pestat] mean and std.dev: (431.95, 90.00) [M::mem_pestat] low and high boundaries for proper pairs: (10, 850) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 120, 2695) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8037) [M::mem_pestat] mean and std.dev: (1206.42, 1684.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10708) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 952.742 CPU sec, 53.319 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 666906, 34, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (418, 1164, 2685) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7219) [M::mem_pestat] mean and std.dev: (1630.83, 1756.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9486) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.23, 90.22) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 72, 2414) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7198) [M::mem_pestat] mean and std.dev: (1206.15, 1699.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9590) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1045, 1267, 3474) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8332) [M::mem_pestat] mean and std.dev: (2716.70, 2737.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13665) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 948.653 CPU sec, 41.977 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 666265, 47, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (347, 532, 929) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2093) [M::mem_pestat] mean and std.dev: (676.38, 537.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2825) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.02, 89.83) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (43, 155, 1829) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5401) [M::mem_pestat] mean and std.dev: (980.04, 1534.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7187) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 948.024 CPU sec, 52.143 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 666772, 33, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (830, 1486, 3594) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9122) [M::mem_pestat] mean and std.dev: (2008.00, 1969.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11886) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (431.96, 89.89) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 92, 3079) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9191) [M::mem_pestat] mean and std.dev: (1379.64, 2020.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12247) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 944.312 CPU sec, 41.777 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666816, 39, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (258, 384, 1472) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3900) [M::mem_pestat] mean and std.dev: (793.00, 849.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5114) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.13, 90.12) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 46, 331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 965) [M::mem_pestat] mean and std.dev: (122.37, 209.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1282) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1142, 1372, 1594) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (238, 2498) [M::mem_pestat] mean and std.dev: (1274.36, 404.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2950) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 945.214 CPU sec, 52.174 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 666310, 46, 10) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.09, 90.05) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 126, 2515) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7493) [M::mem_pestat] mean and std.dev: (1317.43, 1787.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9982) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (579, 1426, 1581) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3585) [M::mem_pestat] mean and std.dev: (1230.10, 649.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4587) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 946.896 CPU sec, 42.013 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 666709, 44, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (399, 944, 1057) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2373) [M::mem_pestat] mean and std.dev: (759.73, 426.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3031) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (431.97, 89.73) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 76, 1682) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5020) [M::mem_pestat] mean and std.dev: (881.71, 1453.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6696) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (650, 969, 3954) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10562) [M::mem_pestat] mean and std.dev: (1624.31, 1422.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13866) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 939.804 CPU sec, 51.828 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 666876, 30, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.16, 89.98) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (10, 36, 2234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6682) [M::mem_pestat] mean and std.dev: (800.82, 1392.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8906) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 934.447 CPU sec, 41.400 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 666246, 38, 14) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.22, 90.15) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 221, 3156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9436) [M::mem_pestat] mean and std.dev: (1853.26, 2614.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12576) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (754, 1334, 2229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5179) [M::mem_pestat] mean and std.dev: (1503.00, 950.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6654) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 941.682 CPU sec, 51.152 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 667386, 33, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (373, 936, 1396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3442) [M::mem_pestat] mean and std.dev: (932.50, 557.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4465) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (370, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (130, 730) [M::mem_pestat] mean and std.dev: (432.11, 90.08) [M::mem_pestat] low and high boundaries for proper pairs: (10, 850) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 73, 2045) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6077) [M::mem_pestat] mean and std.dev: (734.91, 1203.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8093) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1630, 2671, 6378) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15874) [M::mem_pestat] mean and std.dev: (3279.18, 2470.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20622) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 941.533 CPU sec, 41.814 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 666297, 42, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (344, 460, 1034) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2414) [M::mem_pestat] mean and std.dev: (635.93, 374.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3104) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (370, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (130, 730) [M::mem_pestat] mean and std.dev: (432.14, 90.04) [M::mem_pestat] low and high boundaries for proper pairs: (10, 850) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 57, 538) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1582) [M::mem_pestat] mean and std.dev: (111.38, 226.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2104) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 947.511 CPU sec, 49.897 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 666777, 31, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (355, 773, 1241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3013) [M::mem_pestat] mean and std.dev: (661.67, 367.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3899) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.08, 89.80) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 36, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 604) [M::mem_pestat] mean and std.dev: (62.69, 85.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 799) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 939.735 CPU sec, 41.570 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 666726, 32, 13) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.35, 90.04) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 46, 1570) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4674) [M::mem_pestat] mean and std.dev: (918.88, 1432.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6651) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (584, 732, 1798) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4226) [M::mem_pestat] mean and std.dev: (914.00, 564.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5440) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 948.741 CPU sec, 52.631 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 666604, 32, 10) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (370, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (128, 733) [M::mem_pestat] mean and std.dev: (432.21, 90.39) [M::mem_pestat] low and high boundaries for proper pairs: (7, 854) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 35, 659) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1937) [M::mem_pestat] mean and std.dev: (354.55, 598.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2749) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (938, 1145, 2155) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4589) [M::mem_pestat] mean and std.dev: (1496.70, 853.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5806) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 949.536 CPU sec, 42.225 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 666853, 34, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (307, 425, 1755) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4651) [M::mem_pestat] mean and std.dev: (982.10, 995.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6099) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (370, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (130, 730) [M::mem_pestat] mean and std.dev: (432.01, 90.19) [M::mem_pestat] low and high boundaries for proper pairs: (10, 850) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 41, 1607) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4779) [M::mem_pestat] mean and std.dev: (763.81, 1374.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6365) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 950.357 CPU sec, 54.089 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 667226, 40, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (376, 918, 1371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3361) [M::mem_pestat] mean and std.dev: (816.50, 539.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4356) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.06, 89.86) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 46, 2619) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7805) [M::mem_pestat] mean and std.dev: (823.81, 1430.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10398) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (821, 1423, 2700) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6458) [M::mem_pestat] mean and std.dev: (1924.38, 1751.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8929) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 939.649 CPU sec, 41.888 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 667043, 33, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (441, 1318, 3158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8592) [M::mem_pestat] mean and std.dev: (1862.90, 1900.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11309) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (370, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (130, 730) [M::mem_pestat] mean and std.dev: (431.93, 90.06) [M::mem_pestat] low and high boundaries for proper pairs: (10, 850) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 69, 1947) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5789) [M::mem_pestat] mean and std.dev: (692.50, 1304.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7710) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 946.570 CPU sec, 54.225 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 666544, 36, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (734, 1302, 1721) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3695) [M::mem_pestat] mean and std.dev: (966.93, 516.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4682) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.20, 89.95) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 79, 846) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2500) [M::mem_pestat] mean and std.dev: (215.33, 417.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3327) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (418, 1729, 2775) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7489) [M::mem_pestat] mean and std.dev: (1299.18, 1114.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9846) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 943.091 CPU sec, 41.751 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 666716, 30, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (372, 1027, 1948) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5100) [M::mem_pestat] mean and std.dev: (1143.29, 1007.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6676) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.12, 89.85) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 68, 1150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3406) [M::mem_pestat] mean and std.dev: (373.54, 654.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4534) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 943.333 CPU sec, 53.464 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 666842, 44, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.20, 89.93) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 64, 1695) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5037) [M::mem_pestat] mean and std.dev: (699.52, 1189.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6708) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 939.352 CPU sec, 41.584 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 666254, 39, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (441, 907, 1775) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4443) [M::mem_pestat] mean and std.dev: (1079.82, 746.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5777) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.29, 90.14) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 133, 1057) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3111) [M::mem_pestat] mean and std.dev: (432.35, 737.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4138) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (423, 1276, 1582) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3900) [M::mem_pestat] mean and std.dev: (1006.64, 656.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5059) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 941.564 CPU sec, 52.199 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 666504, 36, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (527, 929, 2366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6044) [M::mem_pestat] mean and std.dev: (1381.07, 1189.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7883) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 426, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.05, 90.01) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 45, 1859) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5547) [M::mem_pestat] mean and std.dev: (856.77, 1464.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7391) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (953, 1741, 2646) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6032) [M::mem_pestat] mean and std.dev: (1601.11, 996.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7725) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 939.258 CPU sec, 41.583 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 666665, 35, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (266, 949, 1842) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4994) [M::mem_pestat] mean and std.dev: (1069.27, 1090.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6570) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.27, 90.19) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 141, 3203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9545) [M::mem_pestat] mean and std.dev: (1740.57, 2331.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12716) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 943.311 CPU sec, 51.245 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 667034, 31, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (333, 477, 1804) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4746) [M::mem_pestat] mean and std.dev: (1052.60, 1112.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6217) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.21, 89.94) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (10, 42, 3018) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9034) [M::mem_pestat] mean and std.dev: (1481.39, 2287.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12042) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (813, 1215, 1600) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3174) [M::mem_pestat] mean and std.dev: (1045.85, 442.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3961) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 936.126 CPU sec, 41.265 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 667239, 46, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (358, 1221, 2678) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7318) [M::mem_pestat] mean and std.dev: (1767.50, 2122.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10258) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (370, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (130, 730) [M::mem_pestat] mean and std.dev: (432.09, 90.21) [M::mem_pestat] low and high boundaries for proper pairs: (10, 850) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 130, 1975) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5873) [M::mem_pestat] mean and std.dev: (969.86, 1472.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7822) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 935.860 CPU sec, 51.985 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 666450, 35, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (353, 944, 2550) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6944) [M::mem_pestat] mean and std.dev: (1801.00, 1905.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9423) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (370, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (130, 730) [M::mem_pestat] mean and std.dev: (432.16, 90.22) [M::mem_pestat] low and high boundaries for proper pairs: (10, 850) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (41, 191, 3949) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11765) [M::mem_pestat] mean and std.dev: (1954.26, 2608.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15673) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 940.821 CPU sec, 41.553 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 666877, 33, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (596, 1070, 1569) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3515) [M::mem_pestat] mean and std.dev: (1061.38, 572.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4488) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (370, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (130, 730) [M::mem_pestat] mean and std.dev: (432.03, 90.06) [M::mem_pestat] low and high boundaries for proper pairs: (10, 850) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 47, 1882) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5612) [M::mem_pestat] mean and std.dev: (1044.85, 1493.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7477) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (443, 921, 1492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3590) [M::mem_pestat] mean and std.dev: (1040.93, 717.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4639) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 942.329 CPU sec, 53.843 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 666833, 29, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (287, 641, 2030) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5516) [M::mem_pestat] mean and std.dev: (1248.12, 1463.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7259) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.33, 90.23) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 345, 2735) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8143) [M::mem_pestat] mean and std.dev: (1177.00, 1546.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10847) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (711, 996, 1935) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4383) [M::mem_pestat] mean and std.dev: (1132.10, 863.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5607) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 942.398 CPU sec, 41.906 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 667040, 33, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (337, 517, 1125) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2701) [M::mem_pestat] mean and std.dev: (810.26, 657.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3489) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.11, 89.96) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 461, 2578) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7682) [M::mem_pestat] mean and std.dev: (1018.77, 1456.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10234) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1152, 1645, 2531) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5289) [M::mem_pestat] mean and std.dev: (1709.00, 907.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6668) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 941.452 CPU sec, 51.748 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 666760, 38, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (469, 708, 1775) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4387) [M::mem_pestat] mean and std.dev: (1012.90, 699.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5693) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.08, 89.80) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 47, 2151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6405) [M::mem_pestat] mean and std.dev: (939.00, 1459.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8532) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1253, 2430, 2569) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5201) [M::mem_pestat] mean and std.dev: (2116.70, 801.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6517) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 937.270 CPU sec, 41.435 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 666921, 43, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (374, 520, 1581) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3995) [M::mem_pestat] mean and std.dev: (952.85, 1013.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5202) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.08, 90.16) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 178, 4159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12421) [M::mem_pestat] mean and std.dev: (1910.21, 2513.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16552) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (294, 1373, 2440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6732) [M::mem_pestat] mean and std.dev: (1530.20, 1369.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8878) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 941.786 CPU sec, 53.086 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 667097, 35, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (266, 478, 1092) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2744) [M::mem_pestat] mean and std.dev: (622.93, 435.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3570) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.11, 90.00) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 55, 1914) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5702) [M::mem_pestat] mean and std.dev: (808.19, 1385.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7596) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 931.465 CPU sec, 41.407 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 666581, 47, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (387, 867, 1071) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2439) [M::mem_pestat] mean and std.dev: (755.60, 450.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3123) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.31, 90.05) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 51, 2819) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8411) [M::mem_pestat] mean and std.dev: (1197.48, 1851.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11207) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (725, 1208, 2708) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6674) [M::mem_pestat] mean and std.dev: (1830.00, 1901.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9437) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 936.792 CPU sec, 52.742 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 666856, 43, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (291, 583, 1475) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3843) [M::mem_pestat] mean and std.dev: (995.69, 1038.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5149) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.20, 90.13) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 137, 2964) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8844) [M::mem_pestat] mean and std.dev: (1530.37, 2111.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11784) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1214, 1531, 1913) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3311) [M::mem_pestat] mean and std.dev: (1394.30, 687.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4145) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 931.566 CPU sec, 41.388 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666692, 48, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (275, 316, 1142) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2876) [M::mem_pestat] mean and std.dev: (561.27, 444.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3743) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.42, 90.06) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 55, 1447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4291) [M::mem_pestat] mean and std.dev: (464.44, 864.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5713) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 936.391 CPU sec, 51.633 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 666469, 32, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (490, 933, 1568) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3724) [M::mem_pestat] mean and std.dev: (918.73, 757.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4802) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.26, 90.08) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 780, 2515) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7481) [M::mem_pestat] mean and std.dev: (1357.69, 1615.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9964) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1361, 1743, 1997) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (89, 3269) [M::mem_pestat] mean and std.dev: (1616.91, 794.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4796) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 941.465 CPU sec, 52.255 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 667068, 34, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (403, 511, 1349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3241) [M::mem_pestat] mean and std.dev: (773.23, 609.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4187) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (370, 426, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (130, 730) [M::mem_pestat] mean and std.dev: (432.01, 90.20) [M::mem_pestat] low and high boundaries for proper pairs: (10, 850) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 73, 2469) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7377) [M::mem_pestat] mean and std.dev: (944.03, 1704.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9831) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (389, 575, 1169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2729) [M::mem_pestat] mean and std.dev: (714.89, 671.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3509) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 935.035 CPU sec, 41.464 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 666962, 48, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (229, 669, 761) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1825) [M::mem_pestat] mean and std.dev: (574.22, 415.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2357) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (370, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (130, 730) [M::mem_pestat] mean and std.dev: (432.04, 90.12) [M::mem_pestat] low and high boundaries for proper pairs: (10, 850) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 64, 1457) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4327) [M::mem_pestat] mean and std.dev: (460.09, 1030.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5762) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (580, 1436, 3215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8485) [M::mem_pestat] mean and std.dev: (1269.64, 905.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11120) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 935.351 CPU sec, 50.362 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 667157, 47, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (333, 595, 1047) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2475) [M::mem_pestat] mean and std.dev: (600.00, 358.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3189) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (370, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (130, 730) [M::mem_pestat] mean and std.dev: (432.06, 90.11) [M::mem_pestat] low and high boundaries for proper pairs: (10, 850) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 48, 2416) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7190) [M::mem_pestat] mean and std.dev: (1036.33, 1621.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9577) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1215, 2133, 3199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7167) [M::mem_pestat] mean and std.dev: (2142.90, 1145.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9151) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 936.915 CPU sec, 41.546 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 666466, 42, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (340, 933, 1437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3631) [M::mem_pestat] mean and std.dev: (873.56, 625.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4728) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (370, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (130, 730) [M::mem_pestat] mean and std.dev: (432.02, 90.08) [M::mem_pestat] low and high boundaries for proper pairs: (10, 850) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (11, 47, 1245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3713) [M::mem_pestat] mean and std.dev: (517.10, 902.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4947) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (458, 1201, 2000) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5084) [M::mem_pestat] mean and std.dev: (1347.33, 1292.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6626) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 935.684 CPU sec, 51.179 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 666915, 40, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (365, 521, 1066) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2468) [M::mem_pestat] mean and std.dev: (678.44, 625.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3179) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.13, 90.02) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 197, 2265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6755) [M::mem_pestat] mean and std.dev: (958.92, 1295.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9000) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 929.548 CPU sec, 41.023 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666430, 35, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (369, 428, 530) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (47, 852) [M::mem_pestat] mean and std.dev: (371.00, 158.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1013) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.12, 89.91) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 55, 2777) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8277) [M::mem_pestat] mean and std.dev: (930.67, 1627.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11027) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1055, 1403, 2252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4646) [M::mem_pestat] mean and std.dev: (1419.90, 749.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5843) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 941.449 CPU sec, 52.963 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 666531, 38, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (284, 544, 1204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3044) [M::mem_pestat] mean and std.dev: (892.82, 853.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4305) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.10, 89.90) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 409, 2208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6592) [M::mem_pestat] mean and std.dev: (1176.11, 1669.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8784) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1529, 1877, 2617) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4793) [M::mem_pestat] mean and std.dev: (1646.38, 719.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5881) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 935.662 CPU sec, 41.430 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 667176, 41, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (439, 840, 1309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3049) [M::mem_pestat] mean and std.dev: (842.57, 648.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3919) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.36, 90.09) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 251, 1898) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5636) [M::mem_pestat] mean and std.dev: (1282.37, 1690.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8044) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 938.870 CPU sec, 52.047 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 666480, 33, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (305, 581, 2631) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7283) [M::mem_pestat] mean and std.dev: (1416.12, 1623.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9609) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.06, 89.96) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 42, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 553) [M::mem_pestat] mean and std.dev: (54.22, 67.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 732) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 940.870 CPU sec, 41.608 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 666315, 36, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (370, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (130, 730) [M::mem_pestat] mean and std.dev: (432.07, 90.02) [M::mem_pestat] low and high boundaries for proper pairs: (10, 850) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 159, 4368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13072) [M::mem_pestat] mean and std.dev: (2047.67, 2893.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17424) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 940.550 CPU sec, 52.296 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 667050, 37, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (317, 1160, 2118) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5720) [M::mem_pestat] mean and std.dev: (1018.83, 770.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7521) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.43, 90.02) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (53, 378, 2853) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8453) [M::mem_pestat] mean and std.dev: (1317.39, 1572.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11253) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 933.763 CPU sec, 41.351 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 666920, 34, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (337, 1019, 1836) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4834) [M::mem_pestat] mean and std.dev: (936.55, 629.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6333) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.08, 89.95) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (46, 497, 2495) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7393) [M::mem_pestat] mean and std.dev: (1418.94, 1885.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9842) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 940.772 CPU sec, 53.246 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 666756, 44, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (447, 738, 2993) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8085) [M::mem_pestat] mean and std.dev: (1720.12, 1698.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10631) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.14, 90.04) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 46, 1961) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5837) [M::mem_pestat] mean and std.dev: (753.07, 1170.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7775) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 939.185 CPU sec, 41.543 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 666793, 29, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (300, 551, 2348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6444) [M::mem_pestat] mean and std.dev: (1219.18, 1259.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8492) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.10, 89.94) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 111, 1529) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4523) [M::mem_pestat] mean and std.dev: (776.52, 1193.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6020) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 933.431 CPU sec, 51.892 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 667202, 51, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (259, 442, 2112) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5818) [M::mem_pestat] mean and std.dev: (809.26, 808.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7671) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.36, 90.08) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 42, 775) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2285) [M::mem_pestat] mean and std.dev: (286.78, 555.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3040) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (661, 1108, 2014) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4720) [M::mem_pestat] mean and std.dev: (1114.31, 793.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6073) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 936.685 CPU sec, 41.450 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 666614, 42, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (413, 541, 4774) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13496) [M::mem_pestat] mean and std.dev: (1993.46, 2555.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17857) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.20, 90.08) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 43, 669) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1969) [M::mem_pestat] mean and std.dev: (71.59, 122.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2619) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (780, 1144, 2178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4974) [M::mem_pestat] mean and std.dev: (1378.70, 808.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6372) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 940.506 CPU sec, 52.017 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 666729, 39, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (328, 493, 922) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2110) [M::mem_pestat] mean and std.dev: (517.73, 274.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2704) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.11, 89.98) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 48, 1704) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5074) [M::mem_pestat] mean and std.dev: (932.08, 1586.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7277) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (459, 997, 1348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3126) [M::mem_pestat] mean and std.dev: (950.82, 629.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4015) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 939.607 CPU sec, 41.608 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 666806, 36, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (347, 582, 1277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3137) [M::mem_pestat] mean and std.dev: (747.42, 579.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4067) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.24, 90.14) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 341, 2603) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7743) [M::mem_pestat] mean and std.dev: (1366.77, 1905.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10313) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 942.777 CPU sec, 53.342 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 666558, 39, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (459, 801, 1738) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4296) [M::mem_pestat] mean and std.dev: (874.91, 734.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5575) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.12, 89.97) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 52, 2622) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7822) [M::mem_pestat] mean and std.dev: (863.54, 1432.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10422) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (630, 1338, 1814) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4182) [M::mem_pestat] mean and std.dev: (1389.40, 1070.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5670) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 935.385 CPU sec, 41.494 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 666737, 49, 12) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.24, 89.96) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 40, 1938) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5780) [M::mem_pestat] mean and std.dev: (937.43, 1539.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7701) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1186, 1483, 2256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4396) [M::mem_pestat] mean and std.dev: (1273.60, 732.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5466) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 937.039 CPU sec, 53.904 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 666447, 45, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (386, 969, 1688) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4292) [M::mem_pestat] mean and std.dev: (1056.07, 894.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5594) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.33, 90.07) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 45, 1915) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5709) [M::mem_pestat] mean and std.dev: (900.82, 1491.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7606) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (566, 1045, 1770) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4178) [M::mem_pestat] mean and std.dev: (972.56, 557.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5382) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 941.550 CPU sec, 41.901 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 666847, 32, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (370, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (130, 730) [M::mem_pestat] mean and std.dev: (432.06, 90.15) [M::mem_pestat] low and high boundaries for proper pairs: (10, 850) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 1097, 3637) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10839) [M::mem_pestat] mean and std.dev: (2114.28, 2707.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14440) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 944.265 CPU sec, 53.756 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 665835, 37, 11) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (370, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (130, 730) [M::mem_pestat] mean and std.dev: (432.20, 90.23) [M::mem_pestat] low and high boundaries for proper pairs: (10, 850) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 101, 2611) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7803) [M::mem_pestat] mean and std.dev: (1188.64, 1620.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10399) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (640, 805, 920) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (80, 1480) [M::mem_pestat] mean and std.dev: (665.20, 321.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1951) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 937.993 CPU sec, 41.546 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 666628, 41, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (617, 1333, 3995) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10751) [M::mem_pestat] mean and std.dev: (1983.62, 1868.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14129) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.30, 90.19) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 210, 3264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9744) [M::mem_pestat] mean and std.dev: (1546.76, 2116.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12984) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (510, 1941, 6199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 17577) [M::mem_pestat] mean and std.dev: (3001.85, 2803.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 23266) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 948.956 CPU sec, 53.425 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 667092, 43, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (423, 1105, 1693) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4233) [M::mem_pestat] mean and std.dev: (1042.20, 849.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5503) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.23, 89.95) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 114, 2848) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8480) [M::mem_pestat] mean and std.dev: (1375.86, 2000.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11296) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (994, 1655, 2665) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6007) [M::mem_pestat] mean and std.dev: (1859.91, 1110.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7678) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 949.208 CPU sec, 42.153 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 666092, 40, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (316, 624, 1665) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4363) [M::mem_pestat] mean and std.dev: (1121.06, 1157.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5753) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.17, 89.93) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (8, 28, 1670) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4994) [M::mem_pestat] mean and std.dev: (802.12, 1285.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6656) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1245, 1791, 2901) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6213) [M::mem_pestat] mean and std.dev: (1923.64, 1002.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7869) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 954.285 CPU sec, 55.223 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 666899, 37, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (413, 1479, 1848) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4718) [M::mem_pestat] mean and std.dev: (1047.55, 723.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6153) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.01, 89.92) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 316, 3000) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8962) [M::mem_pestat] mean and std.dev: (1210.11, 1594.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11943) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1146, 1397, 2421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4971) [M::mem_pestat] mean and std.dev: (1596.83, 1073.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6246) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 948.183 CPU sec, 42.197 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 667175, 44, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (417, 568, 1828) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4650) [M::mem_pestat] mean and std.dev: (1061.33, 935.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6061) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.29, 89.95) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 54, 1100) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3264) [M::mem_pestat] mean and std.dev: (403.05, 728.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4346) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 947.978 CPU sec, 54.499 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 666828, 39, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (305, 418, 1437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3701) [M::mem_pestat] mean and std.dev: (715.00, 550.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4833) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.35, 90.17) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 56, 1218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3604) [M::mem_pestat] mean and std.dev: (458.47, 770.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4797) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (369, 1031, 1443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3591) [M::mem_pestat] mean and std.dev: (1041.00, 807.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4665) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 948.363 CPU sec, 42.010 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 667064, 33, 13) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (370, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (130, 730) [M::mem_pestat] mean and std.dev: (432.07, 90.27) [M::mem_pestat] low and high boundaries for proper pairs: (10, 850) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 53, 1451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4311) [M::mem_pestat] mean and std.dev: (400.41, 627.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5741) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (737, 1195, 2147) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4967) [M::mem_pestat] mean and std.dev: (1222.67, 780.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6377) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 941.671 CPU sec, 54.362 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 667048, 40, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (241, 342, 1382) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3664) [M::mem_pestat] mean and std.dev: (676.10, 637.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4805) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.16, 90.00) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 87, 2626) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7838) [M::mem_pestat] mean and std.dev: (1099.15, 1528.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10444) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 943.946 CPU sec, 42.034 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 667290, 39, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (332, 429, 1021) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2399) [M::mem_pestat] mean and std.dev: (592.77, 432.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3088) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.12, 89.95) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 38, 3440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10266) [M::mem_pestat] mean and std.dev: (2010.74, 2856.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13679) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 949.352 CPU sec, 55.502 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666471, 43, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (482, 1100, 2656) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7004) [M::mem_pestat] mean and std.dev: (1712.91, 1720.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9178) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.11, 89.92) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 73, 1806) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5378) [M::mem_pestat] mean and std.dev: (1004.45, 1440.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7164) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 940.725 CPU sec, 41.786 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 666938, 38, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (453, 663, 1453) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3453) [M::mem_pestat] mean and std.dev: (965.33, 892.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4534) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (370, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (130, 730) [M::mem_pestat] mean and std.dev: (432.04, 89.92) [M::mem_pestat] low and high boundaries for proper pairs: (10, 850) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 69, 1850) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5518) [M::mem_pestat] mean and std.dev: (874.74, 1347.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7352) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (619, 1565, 2419) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6019) [M::mem_pestat] mean and std.dev: (1529.94, 1126.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7819) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 951.006 CPU sec, 55.295 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 667016, 32, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.08, 89.91) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 49, 1930) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5760) [M::mem_pestat] mean and std.dev: (1078.34, 1611.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7675) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 942.099 CPU sec, 41.899 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 666545, 35, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (397, 787, 1418) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3460) [M::mem_pestat] mean and std.dev: (925.00, 686.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4481) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.46, 89.94) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 134, 2643) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7871) [M::mem_pestat] mean and std.dev: (1255.74, 1659.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10485) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (968, 1565, 2234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4766) [M::mem_pestat] mean and std.dev: (1422.73, 912.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6032) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 949.775 CPU sec, 54.994 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 666922, 45, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (493, 815, 894) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1696) [M::mem_pestat] mean and std.dev: (708.89, 355.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2130) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.12, 89.94) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 86, 2858) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8518) [M::mem_pestat] mean and std.dev: (1301.34, 1650.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11348) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (444, 1135, 1278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2946) [M::mem_pestat] mean and std.dev: (1162.90, 793.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4338) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 944.400 CPU sec, 42.053 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 666624, 39, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (406, 631, 2738) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7402) [M::mem_pestat] mean and std.dev: (1657.62, 1834.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9734) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (370, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (130, 730) [M::mem_pestat] mean and std.dev: (432.01, 90.02) [M::mem_pestat] low and high boundaries for proper pairs: (10, 850) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 77, 558) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1630) [M::mem_pestat] mean and std.dev: (189.88, 299.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2166) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (675, 1090, 2537) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6261) [M::mem_pestat] mean and std.dev: (1623.33, 1548.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8123) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 950.415 CPU sec, 54.381 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 667332, 47, 11) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.05, 89.80) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 148, 2540) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7556) [M::mem_pestat] mean and std.dev: (1130.73, 1662.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10064) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1215, 1500, 2537) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5181) [M::mem_pestat] mean and std.dev: (1942.45, 1317.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7212) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 943.616 CPU sec, 41.928 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 666851, 35, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (340, 457, 1056) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2488) [M::mem_pestat] mean and std.dev: (642.15, 522.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3204) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 426, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (431.92, 89.80) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 50, 1726) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5108) [M::mem_pestat] mean and std.dev: (836.00, 1355.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6799) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 947.779 CPU sec, 56.018 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 666460, 41, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (345, 644, 1022) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2376) [M::mem_pestat] mean and std.dev: (552.00, 319.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3053) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.15, 89.89) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 54, 1209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3601) [M::mem_pestat] mean and std.dev: (406.97, 747.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4797) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (526, 1108, 1254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2710) [M::mem_pestat] mean and std.dev: (719.22, 436.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3438) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 947.926 CPU sec, 42.163 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 666838, 36, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (442, 974, 1267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2917) [M::mem_pestat] mean and std.dev: (924.00, 695.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3742) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.20, 90.10) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 67, 1733) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5159) [M::mem_pestat] mean and std.dev: (968.47, 1553.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7182) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 945.156 CPU sec, 55.041 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 667080, 37, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (305, 466, 2309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6317) [M::mem_pestat] mean and std.dev: (1350.38, 1425.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8321) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.09, 89.89) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 66, 2893) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8633) [M::mem_pestat] mean and std.dev: (1428.76, 1776.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11503) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 944.267 CPU sec, 41.890 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 666275, 38, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.33, 90.23) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 103, 2780) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8310) [M::mem_pestat] mean and std.dev: (1237.43, 1834.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11075) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 951.853 CPU sec, 56.345 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 666900, 27, 13) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.13, 89.86) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 148, 4340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12950) [M::mem_pestat] mean and std.dev: (1703.48, 2371.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17255) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (766, 1411, 2657) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6439) [M::mem_pestat] mean and std.dev: (1340.92, 1022.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8330) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 947.849 CPU sec, 42.203 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666773, 40, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (324, 388, 2106) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5670) [M::mem_pestat] mean and std.dev: (745.33, 647.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7452) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.02, 89.81) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 110, 1504) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4454) [M::mem_pestat] mean and std.dev: (784.79, 1142.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5929) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 953.700 CPU sec, 56.636 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 667264, 41, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (355, 1256, 1781) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4633) [M::mem_pestat] mean and std.dev: (960.10, 726.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6059) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (370, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (130, 730) [M::mem_pestat] mean and std.dev: (432.16, 90.04) [M::mem_pestat] low and high boundaries for proper pairs: (10, 850) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 54, 821) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2423) [M::mem_pestat] mean and std.dev: (302.11, 491.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3224) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 951.113 CPU sec, 56.657 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 666924, 33, 10) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.34, 90.04) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 76, 2175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6483) [M::mem_pestat] mean and std.dev: (1102.09, 1632.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8637) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (286, 1243, 1354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3490) [M::mem_pestat] mean and std.dev: (983.22, 648.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4558) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 945.115 CPU sec, 42.140 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 667087, 40, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (389, 1170, 1647) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4163) [M::mem_pestat] mean and std.dev: (1145.50, 654.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5421) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.23, 89.92) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 47, 1618) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4800) [M::mem_pestat] mean and std.dev: (758.21, 1392.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6391) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 947.742 CPU sec, 54.933 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 666198, 35, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (453, 1246, 2772) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7410) [M::mem_pestat] mean and std.dev: (1499.77, 1298.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9729) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.34, 90.09) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 261, 2609) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7763) [M::mem_pestat] mean and std.dev: (1343.17, 1544.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10340) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 949.683 CPU sec, 42.240 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 666833, 37, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (312, 1051, 1716) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4524) [M::mem_pestat] mean and std.dev: (1001.47, 697.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5928) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.05, 89.98) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 69, 1618) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4818) [M::mem_pestat] mean and std.dev: (872.89, 1438.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6629) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (388, 1132, 1421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3487) [M::mem_pestat] mean and std.dev: (1023.69, 699.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4520) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 949.885 CPU sec, 56.071 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 666872, 42, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (345, 697, 1123) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2679) [M::mem_pestat] mean and std.dev: (798.93, 656.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3457) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.13, 89.92) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 232, 1908) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5654) [M::mem_pestat] mean and std.dev: (875.46, 1313.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7527) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (692, 1079, 2221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5279) [M::mem_pestat] mean and std.dev: (1075.91, 660.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6808) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 951.470 CPU sec, 42.315 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 666882, 47, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (291, 443, 1409) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3645) [M::mem_pestat] mean and std.dev: (597.77, 449.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4763) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.20, 89.99) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 70, 2429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7247) [M::mem_pestat] mean and std.dev: (1214.36, 1866.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9656) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 947.624 CPU sec, 54.903 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 667023, 39, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (274, 528, 971) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2365) [M::mem_pestat] mean and std.dev: (566.75, 353.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3062) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.20, 90.05) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 30, 1240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3690) [M::mem_pestat] mean and std.dev: (420.89, 873.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4915) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (615, 1120, 1985) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4725) [M::mem_pestat] mean and std.dev: (1093.30, 604.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6095) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 943.472 CPU sec, 42.210 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 667193, 22, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (224, 534, 1667) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4553) [M::mem_pestat] mean and std.dev: (737.85, 690.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5996) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.23, 90.00) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (42, 664, 2144) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6348) [M::mem_pestat] mean and std.dev: (1284.52, 1644.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8450) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (475, 1158, 1813) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4489) [M::mem_pestat] mean and std.dev: (1073.75, 646.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5827) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 940.079 CPU sec, 54.598 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 666652, 32, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (440, 626, 1389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3287) [M::mem_pestat] mean and std.dev: (656.93, 380.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4236) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.36, 90.18) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 214, 2207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6547) [M::mem_pestat] mean and std.dev: (1098.55, 1417.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8717) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 952.304 CPU sec, 42.248 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 666559, 41, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (315, 465, 1054) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2532) [M::mem_pestat] mean and std.dev: (686.69, 469.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3271) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.18, 90.12) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 118, 1615) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4795) [M::mem_pestat] mean and std.dev: (662.48, 1101.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6385) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 950.395 CPU sec, 55.671 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 666956, 48, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (542, 1006, 1858) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4490) [M::mem_pestat] mean and std.dev: (1132.56, 961.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5806) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.25, 89.79) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 125, 1738) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5150) [M::mem_pestat] mean and std.dev: (863.59, 1258.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6856) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (780, 1575, 2664) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6432) [M::mem_pestat] mean and std.dev: (1719.46, 1220.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8316) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 945.763 CPU sec, 42.163 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 667420, 29, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (370, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (130, 730) [M::mem_pestat] mean and std.dev: (432.11, 89.95) [M::mem_pestat] low and high boundaries for proper pairs: (10, 850) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 247, 2975) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8867) [M::mem_pestat] mean and std.dev: (1476.76, 2005.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11813) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 945.964 CPU sec, 54.089 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 666799, 32, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (288, 1130, 2179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5961) [M::mem_pestat] mean and std.dev: (1226.94, 1089.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7852) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.13, 89.90) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 321, 3534) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10544) [M::mem_pestat] mean and std.dev: (1861.47, 2609.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14049) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1027, 1298, 2223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4615) [M::mem_pestat] mean and std.dev: (1420.82, 700.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5811) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 942.265 CPU sec, 41.884 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 667083, 33, 10) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.24, 89.94) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (9, 33, 2199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6579) [M::mem_pestat] mean and std.dev: (873.61, 1507.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8769) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (570, 987, 1654) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3822) [M::mem_pestat] mean and std.dev: (1135.50, 717.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4906) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 944.651 CPU sec, 54.264 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 667126, 48, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (408, 609, 1661) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4167) [M::mem_pestat] mean and std.dev: (814.42, 681.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5420) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.25, 90.21) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 145, 3642) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10882) [M::mem_pestat] mean and std.dev: (1558.33, 1998.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14502) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (682, 1214, 1905) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4351) [M::mem_pestat] mean and std.dev: (1132.17, 665.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5574) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 941.928 CPU sec, 41.849 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 667037, 35, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (694, 1040, 2431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5905) [M::mem_pestat] mean and std.dev: (1108.09, 742.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7642) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.41, 90.05) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 69, 2663) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7957) [M::mem_pestat] mean and std.dev: (1118.73, 1508.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10604) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (257, 1102, 1423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3755) [M::mem_pestat] mean and std.dev: (919.60, 542.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4921) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 944.260 CPU sec, 54.894 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 666728, 40, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (380, 932, 3373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9359) [M::mem_pestat] mean and std.dev: (1704.80, 1863.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12352) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.08, 89.98) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 83, 1236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3646) [M::mem_pestat] mean and std.dev: (549.05, 918.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4851) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (699, 1300, 2586) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6360) [M::mem_pestat] mean and std.dev: (1682.73, 1509.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8247) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 949.245 CPU sec, 42.306 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 666859, 53, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (295, 1057, 3260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9190) [M::mem_pestat] mean and std.dev: (2277.21, 2786.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13424) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.15, 89.89) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 92, 1947) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5805) [M::mem_pestat] mean and std.dev: (995.54, 1357.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7734) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 949.665 CPU sec, 55.745 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 667071, 38, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (311, 477, 1118) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2732) [M::mem_pestat] mean and std.dev: (716.00, 642.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3539) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.29, 90.18) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 214, 2825) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8417) [M::mem_pestat] mean and std.dev: (1336.35, 1692.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11213) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 947.297 CPU sec, 42.335 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 667491, 41, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (430, 702, 2005) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5155) [M::mem_pestat] mean and std.dev: (1224.86, 1136.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6730) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.15, 89.94) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 63, 1417) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4207) [M::mem_pestat] mean and std.dev: (359.69, 701.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5602) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (788, 1659, 2753) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6683) [M::mem_pestat] mean and std.dev: (1413.58, 923.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8648) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 942.467 CPU sec, 55.194 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 666666, 31, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (294, 592, 1124) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2784) [M::mem_pestat] mean and std.dev: (661.79, 401.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3614) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.29, 90.11) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 45, 1628) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4836) [M::mem_pestat] mean and std.dev: (805.52, 1203.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6440) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 944.768 CPU sec, 42.133 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 666993, 33, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (317, 718, 1263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3155) [M::mem_pestat] mean and std.dev: (787.33, 548.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4101) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.09, 89.96) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 41, 1745) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5185) [M::mem_pestat] mean and std.dev: (786.12, 1413.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6905) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (379, 1166, 2483) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6691) [M::mem_pestat] mean and std.dev: (1456.25, 1364.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8795) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 948.040 CPU sec, 55.903 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 666902, 39, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (295, 302, 495) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895) [M::mem_pestat] mean and std.dev: (332.23, 142.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1095) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.07, 89.99) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 30, 1949) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5815) [M::mem_pestat] mean and std.dev: (930.51, 1549.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7748) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (448, 886, 1602) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3910) [M::mem_pestat] mean and std.dev: (1129.00, 903.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5064) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 941.010 CPU sec, 41.929 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 666609, 48, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (322, 528, 2401) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6559) [M::mem_pestat] mean and std.dev: (773.92, 812.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8638) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.18, 89.94) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (44, 628, 2384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7064) [M::mem_pestat] mean and std.dev: (1152.69, 1647.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9404) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (945, 1432, 4172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10626) [M::mem_pestat] mean and std.dev: (2515.94, 2372.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13853) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 950.408 CPU sec, 54.942 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 666858, 30, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (284, 1041, 2624) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7304) [M::mem_pestat] mean and std.dev: (1428.12, 1687.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9644) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (370, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (128, 733) [M::mem_pestat] mean and std.dev: (432.29, 90.30) [M::mem_pestat] low and high boundaries for proper pairs: (7, 854) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 99, 1718) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5128) [M::mem_pestat] mean and std.dev: (847.93, 1254.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6833) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 944.786 CPU sec, 42.115 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666783, 39, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (414, 718, 1319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3129) [M::mem_pestat] mean and std.dev: (601.00, 368.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4034) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.24, 90.20) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 48, 1853) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5515) [M::mem_pestat] mean and std.dev: (854.43, 1473.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7346) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 945.156 CPU sec, 54.020 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 666456, 39, 15) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (370, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (130, 730) [M::mem_pestat] mean and std.dev: (432.00, 90.12) [M::mem_pestat] low and high boundaries for proper pairs: (10, 850) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 41, 640) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1896) [M::mem_pestat] mean and std.dev: (130.44, 267.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2524) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (375, 1367, 2773) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7569) [M::mem_pestat] mean and std.dev: (1461.07, 1163.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9967) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 949.297 CPU sec, 42.190 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666925, 45, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (415, 632, 1806) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4588) [M::mem_pestat] mean and std.dev: (1111.09, 837.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5979) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (370, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (130, 730) [M::mem_pestat] mean and std.dev: (431.99, 90.05) [M::mem_pestat] low and high boundaries for proper pairs: (10, 850) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 105, 1812) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5398) [M::mem_pestat] mean and std.dev: (838.27, 1262.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7191) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (907, 1913, 4115) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10531) [M::mem_pestat] mean and std.dev: (2179.00, 1781.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13739) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 943.621 CPU sec, 53.719 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 666748, 31, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (495, 996, 2010) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5040) [M::mem_pestat] mean and std.dev: (1170.86, 1079.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6555) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (370, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (130, 730) [M::mem_pestat] mean and std.dev: (432.01, 90.14) [M::mem_pestat] low and high boundaries for proper pairs: (10, 850) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 1273, 3000) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8926) [M::mem_pestat] mean and std.dev: (1813.97, 2305.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11889) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (637, 1147, 2137) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5137) [M::mem_pestat] mean and std.dev: (938.20, 583.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6637) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 948.061 CPU sec, 42.186 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666761, 31, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (395, 439, 803) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1619) [M::mem_pestat] mean and std.dev: (495.30, 221.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2027) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.22, 89.93) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 50, 1472) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4392) [M::mem_pestat] mean and std.dev: (755.79, 1237.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5852) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 944.991 CPU sec, 56.191 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 667290, 31, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (334, 821, 1436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3640) [M::mem_pestat] mean and std.dev: (885.43, 653.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4742) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.31, 89.98) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (45, 122, 2600) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7710) [M::mem_pestat] mean and std.dev: (1275.29, 1752.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10265) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (933, 1213, 1892) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3810) [M::mem_pestat] mean and std.dev: (1250.67, 865.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4769) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 944.307 CPU sec, 41.965 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 667225, 38, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (350, 417, 1387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3461) [M::mem_pestat] mean and std.dev: (795.85, 685.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4498) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.21, 90.08) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 50, 1350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4016) [M::mem_pestat] mean and std.dev: (734.83, 1156.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5362) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 948.490 CPU sec, 54.836 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 667138, 35, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (413, 710, 2194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5756) [M::mem_pestat] mean and std.dev: (1377.50, 1448.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7537) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (370, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (130, 730) [M::mem_pestat] mean and std.dev: (432.15, 90.14) [M::mem_pestat] low and high boundaries for proper pairs: (10, 850) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 69, 2709) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8077) [M::mem_pestat] mean and std.dev: (1199.12, 1703.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10761) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 947.492 CPU sec, 42.105 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 666886, 33, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (356, 562, 1213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2927) [M::mem_pestat] mean and std.dev: (646.18, 401.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3784) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.10, 89.95) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 42, 1830) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5458) [M::mem_pestat] mean and std.dev: (762.87, 1409.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7272) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 946.580 CPU sec, 55.162 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 666558, 41, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (381, 625, 1259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3015) [M::mem_pestat] mean and std.dev: (700.11, 531.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3893) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.08, 89.91) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 62, 1353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3991) [M::mem_pestat] mean and std.dev: (414.73, 729.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5310) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1004, 2000, 2141) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4415) [M::mem_pestat] mean and std.dev: (1927.50, 1016.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5994) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 945.236 CPU sec, 41.971 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 666844, 30, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (376, 1142, 4115) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11593) [M::mem_pestat] mean and std.dev: (1963.38, 2125.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15332) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.40, 90.12) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 57, 2212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6596) [M::mem_pestat] mean and std.dev: (1060.93, 1535.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8788) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (342, 1214, 2676) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7344) [M::mem_pestat] mean and std.dev: (1707.50, 1739.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9678) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 950.680 CPU sec, 54.605 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 666902, 26, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (317, 769, 1631) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4259) [M::mem_pestat] mean and std.dev: (1027.38, 1009.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5573) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (370, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (130, 730) [M::mem_pestat] mean and std.dev: (432.00, 90.08) [M::mem_pestat] low and high boundaries for proper pairs: (10, 850) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 68, 502) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1480) [M::mem_pestat] mean and std.dev: (150.57, 227.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1969) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (404, 1018, 1500) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3692) [M::mem_pestat] mean and std.dev: (1039.50, 696.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4788) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 942.380 CPU sec, 42.005 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 667102, 32, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (640, 793, 1585) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3475) [M::mem_pestat] mean and std.dev: (1108.27, 844.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4487) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (370, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (128, 733) [M::mem_pestat] mean and std.dev: (432.23, 90.30) [M::mem_pestat] low and high boundaries for proper pairs: (7, 854) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 107, 2975) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8881) [M::mem_pestat] mean and std.dev: (1449.81, 2334.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11834) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 946.705 CPU sec, 55.752 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 667094, 43, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (246, 387, 1096) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2796) [M::mem_pestat] mean and std.dev: (672.31, 528.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3646) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.09, 90.04) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 86, 1795) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5323) [M::mem_pestat] mean and std.dev: (1008.31, 1525.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7109) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 942.469 CPU sec, 41.867 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 666979, 36, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (280, 358, 796) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1828) [M::mem_pestat] mean and std.dev: (517.62, 336.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2344) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.05, 89.96) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 77, 1453) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4317) [M::mem_pestat] mean and std.dev: (777.94, 1182.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5749) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (782, 1206, 2769) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6743) [M::mem_pestat] mean and std.dev: (1673.36, 1199.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8730) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 946.027 CPU sec, 55.358 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 667255, 40, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (393, 697, 2765) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7509) [M::mem_pestat] mean and std.dev: (1397.41, 1436.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9881) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (370, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (128, 733) [M::mem_pestat] mean and std.dev: (432.26, 90.35) [M::mem_pestat] low and high boundaries for proper pairs: (7, 854) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 61, 2393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7135) [M::mem_pestat] mean and std.dev: (1010.28, 1686.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9506) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (248, 1228, 1697) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4595) [M::mem_pestat] mean and std.dev: (1063.81, 772.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6044) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 945.101 CPU sec, 42.067 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 666724, 33, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (450, 1226, 2708) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7224) [M::mem_pestat] mean and std.dev: (1676.19, 1592.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9482) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.33, 90.14) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 43, 948) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2804) [M::mem_pestat] mean and std.dev: (214.30, 531.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3732) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (320, 963, 1278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3194) [M::mem_pestat] mean and std.dev: (929.83, 801.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4152) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 952.348 CPU sec, 55.136 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 667088, 39, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (291, 654, 1346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3456) [M::mem_pestat] mean and std.dev: (744.33, 785.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4511) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.11, 89.95) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 46, 1734) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5176) [M::mem_pestat] mean and std.dev: (826.51, 1431.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6897) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 946.292 CPU sec, 42.016 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 666950, 28, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (274, 386, 1680) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4492) [M::mem_pestat] mean and std.dev: (743.93, 971.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5898) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.17, 89.86) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 93, 1013) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2975) [M::mem_pestat] mean and std.dev: (317.35, 620.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3956) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 946.021 CPU sec, 55.233 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 666349, 36, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (425, 777, 1910) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4880) [M::mem_pestat] mean and std.dev: (1229.00, 1268.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6365) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.34, 90.30) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 104, 1206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3586) [M::mem_pestat] mean and std.dev: (413.68, 742.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4776) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (960, 1353, 1506) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2598) [M::mem_pestat] mean and std.dev: (1120.33, 399.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3144) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 939.218 CPU sec, 41.716 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 666995, 37, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (330, 620, 1185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2895) [M::mem_pestat] mean and std.dev: (720.17, 525.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3750) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.29, 89.92) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 55, 2276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6790) [M::mem_pestat] mean and std.dev: (929.83, 1412.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9047) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (906, 1215, 2698) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6282) [M::mem_pestat] mean and std.dev: (1715.00, 1304.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8074) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 945.088 CPU sec, 55.070 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 666886, 41, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (366, 710, 1408) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3492) [M::mem_pestat] mean and std.dev: (990.00, 975.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4893) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.31, 90.19) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (38, 362, 3415) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10169) [M::mem_pestat] mean and std.dev: (1614.02, 2014.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13546) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 945.732 CPU sec, 42.051 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 667112, 39, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (381, 1719, 2634) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7140) [M::mem_pestat] mean and std.dev: (1609.07, 1733.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9393) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.28, 90.11) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (9, 25, 143) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (40.97, 67.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 545) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1245, 1800, 2539) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5127) [M::mem_pestat] mean and std.dev: (1743.71, 998.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6421) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 942.891 CPU sec, 53.402 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 666804, 45, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (364, 604, 2330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6262) [M::mem_pestat] mean and std.dev: (1096.50, 1269.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8228) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (370, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (130, 730) [M::mem_pestat] mean and std.dev: (432.14, 89.97) [M::mem_pestat] low and high boundaries for proper pairs: (10, 850) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 38, 1789) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5341) [M::mem_pestat] mean and std.dev: (884.41, 1548.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7117) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 943.904 CPU sec, 41.989 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 667201, 45, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (465, 612, 1214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2712) [M::mem_pestat] mean and std.dev: (633.07, 291.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3461) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.25, 90.14) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 86, 2691) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8027) [M::mem_pestat] mean and std.dev: (1098.93, 1663.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10695) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 946.720 CPU sec, 54.422 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 667060, 46, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (313, 633, 1792) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4750) [M::mem_pestat] mean and std.dev: (996.13, 833.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6229) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.09, 89.88) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 88, 2661) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7939) [M::mem_pestat] mean and std.dev: (1280.24, 1838.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10578) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 945.397 CPU sec, 42.238 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666654, 36, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (400, 911, 1537) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3811) [M::mem_pestat] mean and std.dev: (912.80, 591.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4948) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.14, 89.98) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 37, 662) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1962) [M::mem_pestat] mean and std.dev: (273.21, 538.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2612) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (741, 1299, 1911) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4251) [M::mem_pestat] mean and std.dev: (1220.00, 668.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5421) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 947.256 CPU sec, 54.939 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 666633, 38, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (461, 1150, 3309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9005) [M::mem_pestat] mean and std.dev: (2088.80, 2114.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11853) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.21, 90.10) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 450, 3226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9638) [M::mem_pestat] mean and std.dev: (1692.84, 2161.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12844) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (749, 1128, 2851) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7055) [M::mem_pestat] mean and std.dev: (1431.58, 1002.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9157) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 951.237 CPU sec, 42.392 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 667366, 47, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (283, 821, 2084) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5686) [M::mem_pestat] mean and std.dev: (1119.64, 1019.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7487) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.27, 90.21) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 130, 1779) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5293) [M::mem_pestat] mean and std.dev: (750.47, 1282.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7050) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (898, 1369, 2481) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5647) [M::mem_pestat] mean and std.dev: (1267.60, 637.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7230) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 942.412 CPU sec, 53.948 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 666382, 44, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (220, 522, 1128) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2944) [M::mem_pestat] mean and std.dev: (587.33, 394.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3852) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.15, 89.89) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 50, 2856) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8532) [M::mem_pestat] mean and std.dev: (1352.00, 2136.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11370) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1103, 1194, 1854) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3356) [M::mem_pestat] mean and std.dev: (1159.67, 580.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4107) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 948.360 CPU sec, 42.282 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 666466, 37, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (938, 1406, 2830) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6614) [M::mem_pestat] mean and std.dev: (1720.00, 1534.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8506) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.35, 90.01) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 73, 3079) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9199) [M::mem_pestat] mean and std.dev: (1439.92, 2100.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12259) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 947.889 CPU sec, 56.056 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 667087, 26, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (279, 766, 1850) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4992) [M::mem_pestat] mean and std.dev: (1232.08, 1416.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6898) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.19, 89.99) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 61, 1979) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5897) [M::mem_pestat] mean and std.dev: (900.54, 1476.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7856) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (500, 976, 1465) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3395) [M::mem_pestat] mean and std.dev: (904.33, 496.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4360) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 948.611 CPU sec, 42.157 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666995, 44, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (660, 1173, 1875) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4305) [M::mem_pestat] mean and std.dev: (1240.09, 815.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5520) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.18, 89.91) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 60, 2352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7010) [M::mem_pestat] mean and std.dev: (920.93, 1492.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9339) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (436, 1286, 1551) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3781) [M::mem_pestat] mean and std.dev: (1220.00, 746.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4896) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 944.055 CPU sec, 53.528 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 667260, 36, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (448, 944, 2292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5980) [M::mem_pestat] mean and std.dev: (1423.77, 1355.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7824) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (370, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (130, 730) [M::mem_pestat] mean and std.dev: (432.07, 90.01) [M::mem_pestat] low and high boundaries for proper pairs: (10, 850) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 144, 2700) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8036) [M::mem_pestat] mean and std.dev: (1201.66, 1627.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10704) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 941.828 CPU sec, 55.385 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 666473, 29, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (303, 365, 2199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5991) [M::mem_pestat] mean and std.dev: (1204.31, 1317.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7887) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.02, 89.90) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 122, 1854) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5534) [M::mem_pestat] mean and std.dev: (979.07, 1529.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7374) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (992, 1295, 2272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4832) [M::mem_pestat] mean and std.dev: (1669.27, 1146.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6257) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 950.488 CPU sec, 42.307 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 666911, 43, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (258, 907, 2989) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8451) [M::mem_pestat] mean and std.dev: (1622.69, 1875.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11182) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.14, 89.99) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 126, 2387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7093) [M::mem_pestat] mean and std.dev: (1131.20, 1507.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9446) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (832, 1339, 2408) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5560) [M::mem_pestat] mean and std.dev: (1586.64, 1051.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7136) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 941.949 CPU sec, 53.669 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 667185, 43, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (393, 779, 2841) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7737) [M::mem_pestat] mean and std.dev: (1473.64, 1767.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10185) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.09, 90.02) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 52, 1521) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4517) [M::mem_pestat] mean and std.dev: (687.58, 1129.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6015) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (648, 948, 3777) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10035) [M::mem_pestat] mean and std.dev: (2087.36, 2509.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13164) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 942.728 CPU sec, 41.993 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 667432, 43, 13) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (370, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (130, 730) [M::mem_pestat] mean and std.dev: (432.01, 90.16) [M::mem_pestat] low and high boundaries for proper pairs: (10, 850) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 80, 2647) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7911) [M::mem_pestat] mean and std.dev: (1045.46, 1668.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10543) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (795, 1008, 2733) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6609) [M::mem_pestat] mean and std.dev: (1886.38, 1820.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9167) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 950.258 CPU sec, 55.114 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 666776, 36, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (253, 370, 1530) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4084) [M::mem_pestat] mean and std.dev: (648.55, 558.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5361) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.18, 90.03) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (41, 150, 2771) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8231) [M::mem_pestat] mean and std.dev: (1367.03, 1885.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10961) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 945.310 CPU sec, 41.969 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 666260, 51, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (323, 771, 1661) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4337) [M::mem_pestat] mean and std.dev: (989.85, 944.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5675) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.06, 89.99) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 47, 1817) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5411) [M::mem_pestat] mean and std.dev: (928.35, 1438.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7208) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 952.811 CPU sec, 55.473 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 666991, 47, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (619, 906, 1113) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2101) [M::mem_pestat] mean and std.dev: (852.70, 423.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2595) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.60, 90.10) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 131, 2877) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8583) [M::mem_pestat] mean and std.dev: (1381.53, 2120.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11436) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (832, 1243, 2032) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4432) [M::mem_pestat] mean and std.dev: (1385.50, 1134.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5922) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 946.342 CPU sec, 41.860 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 667172, 38, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (298, 394, 569) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1111) [M::mem_pestat] mean and std.dev: (421.67, 221.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1382) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.16, 89.89) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 63, 1485) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4409) [M::mem_pestat] mean and std.dev: (746.41, 1354.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6163) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1199, 1314, 2379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4739) [M::mem_pestat] mean and std.dev: (1656.31, 820.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5919) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 945.204 CPU sec, 53.748 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 666923, 51, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (227, 433, 634) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1448) [M::mem_pestat] mean and std.dev: (451.89, 365.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1912) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.06, 89.79) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 64, 2096) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6252) [M::mem_pestat] mean and std.dev: (1114.96, 1620.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8330) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 943.673 CPU sec, 41.918 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666795, 35, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (397, 813, 1036) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2314) [M::mem_pestat] mean and std.dev: (711.09, 325.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2953) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.10, 89.88) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 95, 2602) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7742) [M::mem_pestat] mean and std.dev: (1209.50, 1604.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10312) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (716, 1031, 2377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5699) [M::mem_pestat] mean and std.dev: (1067.20, 614.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7360) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 949.814 CPU sec, 56.648 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 666681, 38, 11) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (431.94, 89.95) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 1313, 2598) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7720) [M::mem_pestat] mean and std.dev: (1710.79, 1954.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10281) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (919, 941, 2082) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4408) [M::mem_pestat] mean and std.dev: (1112.40, 801.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5571) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 942.694 CPU sec, 41.777 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 666442, 37, 14) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (370, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (130, 730) [M::mem_pestat] mean and std.dev: (432.15, 90.11) [M::mem_pestat] low and high boundaries for proper pairs: (10, 850) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 33, 773) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2293) [M::mem_pestat] mean and std.dev: (154.71, 356.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3053) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (961, 1456, 1750) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3328) [M::mem_pestat] mean and std.dev: (1063.09, 548.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4117) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 952.950 CPU sec, 55.917 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 667114, 35, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (252, 357, 788) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1860) [M::mem_pestat] mean and std.dev: (458.18, 344.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2396) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.25, 89.99) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 311, 3294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9842) [M::mem_pestat] mean and std.dev: (2029.46, 2687.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13116) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (914, 1106, 1880) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3812) [M::mem_pestat] mean and std.dev: (1229.94, 716.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4778) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 945.905 CPU sec, 42.050 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 666536, 50, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (299, 614, 1224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3074) [M::mem_pestat] mean and std.dev: (693.50, 553.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3999) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.23, 90.01) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 676, 2846) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8464) [M::mem_pestat] mean and std.dev: (1441.31, 1826.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11273) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (245, 751, 1340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3530) [M::mem_pestat] mean and std.dev: (940.80, 971.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4829) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 950.486 CPU sec, 55.650 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 666922, 45, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (473, 567, 1446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3392) [M::mem_pestat] mean and std.dev: (803.09, 670.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4365) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.17, 90.03) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 88, 1885) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5611) [M::mem_pestat] mean and std.dev: (924.19, 1476.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7474) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (522, 989, 2279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5793) [M::mem_pestat] mean and std.dev: (1267.06, 1093.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7550) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 941.248 CPU sec, 41.793 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 666609, 38, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (406, 506, 1620) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4048) [M::mem_pestat] mean and std.dev: (994.38, 1065.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5262) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.09, 89.94) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 125, 1880) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5590) [M::mem_pestat] mean and std.dev: (858.86, 1266.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7445) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 945.352 CPU sec, 56.074 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 666842, 50, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (429, 1093, 1637) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4053) [M::mem_pestat] mean and std.dev: (1029.73, 600.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5261) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.10, 89.94) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 263, 3395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10143) [M::mem_pestat] mean and std.dev: (1777.18, 2127.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13517) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (301, 1592, 2387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6559) [M::mem_pestat] mean and std.dev: (1821.30, 1447.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8645) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 943.463 CPU sec, 42.059 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666896, 37, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (492, 1806, 3819) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10473) [M::mem_pestat] mean and std.dev: (2616.82, 2916.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14282) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.14, 89.99) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 149, 2629) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7859) [M::mem_pestat] mean and std.dev: (1202.44, 1731.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10474) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (268, 896, 3136) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8872) [M::mem_pestat] mean and std.dev: (1702.85, 1981.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11740) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 948.923 CPU sec, 56.145 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 666392, 39, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (420, 579, 2121) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5523) [M::mem_pestat] mean and std.dev: (1093.08, 1023.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7224) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.25, 89.97) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 36, 1496) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4450) [M::mem_pestat] mean and std.dev: (681.43, 1193.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5927) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (938, 1274, 2411) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5357) [M::mem_pestat] mean and std.dev: (1792.60, 1483.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7725) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 942.334 CPU sec, 41.885 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 666434, 30, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (649, 1088, 2154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5164) [M::mem_pestat] mean and std.dev: (1484.50, 1327.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6795) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 426, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (431.92, 89.79) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 198, 1335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3961) [M::mem_pestat] mean and std.dev: (386.60, 558.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5274) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (416, 876, 2111) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5501) [M::mem_pestat] mean and std.dev: (1145.82, 898.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7196) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 948.967 CPU sec, 54.634 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 666555, 37, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (275, 392, 641) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1373) [M::mem_pestat] mean and std.dev: (432.56, 210.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1739) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 426, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (431.98, 89.86) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 60, 2388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7112) [M::mem_pestat] mean and std.dev: (823.61, 1338.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9474) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (748, 1009, 2333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5503) [M::mem_pestat] mean and std.dev: (1360.29, 1010.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7088) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 936.077 CPU sec, 41.512 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 666390, 39, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.06, 89.99) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 49, 2579) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7707) [M::mem_pestat] mean and std.dev: (872.37, 1460.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10271) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 947.836 CPU sec, 53.990 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 666509, 40, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (396, 1369, 2493) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6687) [M::mem_pestat] mean and std.dev: (1608.53, 1621.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8784) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.08, 89.99) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 56, 1948) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5784) [M::mem_pestat] mean and std.dev: (928.92, 1511.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7702) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 940.748 CPU sec, 41.774 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 666988, 35, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (267, 350, 646) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1404) [M::mem_pestat] mean and std.dev: (375.08, 174.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1783) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.15, 89.94) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 76, 1323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3915) [M::mem_pestat] mean and std.dev: (311.59, 592.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5211) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1019, 1536, 2826) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6440) [M::mem_pestat] mean and std.dev: (1836.10, 1148.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8247) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 942.820 CPU sec, 53.767 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 667080, 41, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (349, 917, 1443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3631) [M::mem_pestat] mean and std.dev: (938.08, 827.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4725) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.06, 90.07) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (11, 32, 667) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1979) [M::mem_pestat] mean and std.dev: (197.32, 427.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2635) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 948.216 CPU sec, 42.446 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 666915, 55, 11) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.11, 90.03) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 169, 1392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4116) [M::mem_pestat] mean and std.dev: (650.84, 1049.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5478) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1645, 2694, 4308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9634) [M::mem_pestat] mean and std.dev: (3022.82, 1947.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12297) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 942.998 CPU sec, 53.900 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 666593, 41, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (302, 900, 2560) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7076) [M::mem_pestat] mean and std.dev: (1359.18, 1948.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9334) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.21, 89.90) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 1301, 3014) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8970) [M::mem_pestat] mean and std.dev: (1818.45, 2142.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11948) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 949.343 CPU sec, 42.192 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 666867, 34, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (359, 522, 1289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3149) [M::mem_pestat] mean and std.dev: (745.20, 561.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4079) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.07, 90.02) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 93, 1379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4093) [M::mem_pestat] mean and std.dev: (625.88, 961.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5450) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 949.976 CPU sec, 54.701 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 666552, 35, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (359, 643, 1106) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2600) [M::mem_pestat] mean and std.dev: (754.75, 620.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3347) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.24, 90.08) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 80, 1746) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5192) [M::mem_pestat] mean and std.dev: (997.69, 1515.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7060) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 943.529 CPU sec, 42.037 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 666950, 40, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (404, 816, 1945) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5027) [M::mem_pestat] mean and std.dev: (1049.90, 846.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6568) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.20, 90.16) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 42, 1683) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5011) [M::mem_pestat] mean and std.dev: (804.82, 1381.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6675) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 945.794 CPU sec, 54.802 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666492, 42, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (319, 1098, 1972) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5278) [M::mem_pestat] mean and std.dev: (1262.18, 1162.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6931) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.14, 89.92) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (49, 1145, 2636) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7810) [M::mem_pestat] mean and std.dev: (1267.25, 1321.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10397) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 940.299 CPU sec, 41.788 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 666782, 48, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (290, 422, 1532) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4016) [M::mem_pestat] mean and std.dev: (729.68, 668.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5258) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.40, 90.11) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 502, 4351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12981) [M::mem_pestat] mean and std.dev: (2032.62, 2656.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17296) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (376, 1414, 2416) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6496) [M::mem_pestat] mean and std.dev: (1425.10, 1081.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8536) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 947.820 CPU sec, 55.127 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 666844, 42, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (262, 771, 2078) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5710) [M::mem_pestat] mean and std.dev: (1051.07, 1019.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7526) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (370, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (130, 730) [M::mem_pestat] mean and std.dev: (432.17, 90.15) [M::mem_pestat] low and high boundaries for proper pairs: (10, 850) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (9, 36, 1218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3636) [M::mem_pestat] mean and std.dev: (377.22, 817.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4845) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 948.719 CPU sec, 42.252 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 666459, 32, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (301, 425, 1804) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4810) [M::mem_pestat] mean and std.dev: (654.42, 628.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6313) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (370, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (130, 730) [M::mem_pestat] mean and std.dev: (432.02, 90.02) [M::mem_pestat] low and high boundaries for proper pairs: (10, 850) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 119, 3240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9656) [M::mem_pestat] mean and std.dev: (1396.59, 1932.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12864) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 950.120 CPU sec, 54.257 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 666487, 32, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (869, 1047, 1514) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2804) [M::mem_pestat] mean and std.dev: (971.42, 449.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3449) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (370, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (130, 730) [M::mem_pestat] mean and std.dev: (431.94, 90.04) [M::mem_pestat] low and high boundaries for proper pairs: (10, 850) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (52, 988, 2672) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7912) [M::mem_pestat] mean and std.dev: (1465.97, 1676.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10532) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 946.884 CPU sec, 42.112 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 667125, 43, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (292, 778, 1470) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3826) [M::mem_pestat] mean and std.dev: (817.71, 627.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5004) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.24, 89.99) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 115, 1970) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5864) [M::mem_pestat] mean and std.dev: (902.85, 1364.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7811) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1196, 2125, 4123) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9977) [M::mem_pestat] mean and std.dev: (2303.17, 1607.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12904) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 938.416 CPU sec, 53.313 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 666841, 51, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (319, 1047, 1602) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4168) [M::mem_pestat] mean and std.dev: (1197.06, 1164.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5856) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.32, 90.14) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 43, 1948) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5812) [M::mem_pestat] mean and std.dev: (896.70, 1495.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7744) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 945.202 CPU sec, 42.072 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 667063, 39, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.03, 89.97) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 40, 869) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2571) [M::mem_pestat] mean and std.dev: (206.78, 444.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3422) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 946.234 CPU sec, 54.459 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 667105, 45, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (315, 782, 1692) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4446) [M::mem_pestat] mean and std.dev: (811.67, 785.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5823) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (370, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (128, 733) [M::mem_pestat] mean and std.dev: (432.28, 90.31) [M::mem_pestat] low and high boundaries for proper pairs: (7, 854) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 41, 569) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1671) [M::mem_pestat] mean and std.dev: (163.95, 279.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2222) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (673, 947, 1838) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4168) [M::mem_pestat] mean and std.dev: (1106.33, 840.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5333) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 945.131 CPU sec, 42.046 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 666256, 33, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (387, 788, 1550) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3876) [M::mem_pestat] mean and std.dev: (861.25, 668.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5039) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.25, 89.99) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (10, 39, 2885) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8635) [M::mem_pestat] mean and std.dev: (915.33, 1504.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11510) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 947.934 CPU sec, 53.952 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 666902, 40, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (380, 919, 1118) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2594) [M::mem_pestat] mean and std.dev: (646.75, 367.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3332) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (370, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (130, 730) [M::mem_pestat] mean and std.dev: (432.12, 90.10) [M::mem_pestat] low and high boundaries for proper pairs: (10, 850) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 34, 1877) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5601) [M::mem_pestat] mean and std.dev: (1016.23, 1562.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7463) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (735, 871, 1526) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3108) [M::mem_pestat] mean and std.dev: (990.00, 571.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3899) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 945.559 CPU sec, 41.938 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 667325, 42, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (581, 995, 1405) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3053) [M::mem_pestat] mean and std.dev: (896.21, 562.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3877) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.20, 89.95) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 37, 1734) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5172) [M::mem_pestat] mean and std.dev: (747.27, 1356.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6891) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (396, 1049, 1584) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3960) [M::mem_pestat] mean and std.dev: (927.78, 516.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5148) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 941.356 CPU sec, 55.157 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 666903, 31, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (424, 1493, 1762) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4438) [M::mem_pestat] mean and std.dev: (1394.08, 1135.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5937) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.38, 90.14) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 36, 1628) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4854) [M::mem_pestat] mean and std.dev: (795.90, 1362.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6467) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 946.361 CPU sec, 42.109 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 666971, 42, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1134, 1688, 2173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4251) [M::mem_pestat] mean and std.dev: (1252.38, 664.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5290) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.00, 89.80) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 319, 2859) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8513) [M::mem_pestat] mean and std.dev: (1319.20, 1671.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11340) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 950.787 CPU sec, 55.073 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 666838, 38, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (394, 764, 1532) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3808) [M::mem_pestat] mean and std.dev: (958.47, 674.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4946) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.42, 90.02) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (10, 47, 1951) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5833) [M::mem_pestat] mean and std.dev: (1069.70, 1724.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7966) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 949.463 CPU sec, 42.273 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 667210, 33, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (377, 768, 1948) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5090) [M::mem_pestat] mean and std.dev: (1101.14, 964.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6661) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (370, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (130, 730) [M::mem_pestat] mean and std.dev: (432.07, 90.10) [M::mem_pestat] low and high boundaries for proper pairs: (10, 850) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 78, 609) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1773) [M::mem_pestat] mean and std.dev: (211.57, 375.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2355) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 948.477 CPU sec, 55.216 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 666806, 32, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (268, 312, 1116) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2812) [M::mem_pestat] mean and std.dev: (581.06, 504.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3660) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.32, 90.17) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 59, 1489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4421) [M::mem_pestat] mean and std.dev: (621.90, 1035.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5887) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1200, 1387, 1660) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (280, 2580) [M::mem_pestat] mean and std.dev: (1445.22, 363.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3040) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 945.917 CPU sec, 54.308 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 667189, 40, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (859, 1242, 1934) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4084) [M::mem_pestat] mean and std.dev: (1294.00, 774.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5159) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (370, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (130, 730) [M::mem_pestat] mean and std.dev: (431.95, 90.04) [M::mem_pestat] low and high boundaries for proper pairs: (10, 850) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 112, 1978) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5880) [M::mem_pestat] mean and std.dev: (986.32, 1450.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7831) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 936.805 CPU sec, 41.663 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 666795, 42, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (354, 913, 1319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3249) [M::mem_pestat] mean and std.dev: (852.12, 659.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4214) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.22, 89.99) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 119, 2430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7248) [M::mem_pestat] mean and std.dev: (1180.70, 1708.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9657) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (267, 1339, 2088) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5730) [M::mem_pestat] mean and std.dev: (1250.00, 1166.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7551) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 948.327 CPU sec, 54.545 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 667402, 41, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (303, 551, 1182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2940) [M::mem_pestat] mean and std.dev: (697.40, 444.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3819) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.14, 89.95) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 139, 1439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4269) [M::mem_pestat] mean and std.dev: (531.27, 707.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5684) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 940.486 CPU sec, 41.812 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 666781, 32, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (349, 519, 1688) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4366) [M::mem_pestat] mean and std.dev: (993.50, 1097.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5705) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.09, 90.01) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 142, 1503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4445) [M::mem_pestat] mean and std.dev: (782.79, 1314.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6040) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (857, 1190, 1804) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3698) [M::mem_pestat] mean and std.dev: (1149.55, 608.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4645) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 947.913 CPU sec, 54.559 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 666597, 32, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (298, 466, 1531) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3997) [M::mem_pestat] mean and std.dev: (831.25, 797.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5230) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.16, 90.06) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (41, 326, 2112) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6254) [M::mem_pestat] mean and std.dev: (870.76, 1275.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8325) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 950.690 CPU sec, 42.469 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666845, 36, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (364, 712, 1505) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3787) [M::mem_pestat] mean and std.dev: (800.40, 582.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4928) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.29, 89.91) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 73, 1902) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5670) [M::mem_pestat] mean and std.dev: (720.12, 1335.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7554) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 944.248 CPU sec, 54.429 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 667036, 48, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (386, 627, 2227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5909) [M::mem_pestat] mean and std.dev: (1313.73, 1300.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7750) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.29, 90.14) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 303, 4363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13035) [M::mem_pestat] mean and std.dev: (1585.23, 2062.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17371) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 943.235 CPU sec, 41.962 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 666415, 34, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (276, 562, 2566) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7146) [M::mem_pestat] mean and std.dev: (1158.07, 1443.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9436) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.30, 90.04) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 115, 1868) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5564) [M::mem_pestat] mean and std.dev: (954.74, 1363.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7412) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (727, 1056, 1445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2881) [M::mem_pestat] mean and std.dev: (1075.46, 596.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3599) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 955.289 CPU sec, 56.028 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 666962, 42, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (340, 648, 1489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3787) [M::mem_pestat] mean and std.dev: (885.31, 770.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4936) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.10, 90.03) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 38, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631) [M::mem_pestat] mean and std.dev: (67.54, 82.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 836) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 943.408 CPU sec, 41.844 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 666709, 47, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (247, 454, 1879) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5143) [M::mem_pestat] mean and std.dev: (1128.08, 1309.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6775) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.12, 90.01) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 31, 1665) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4965) [M::mem_pestat] mean and std.dev: (929.11, 1553.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7143) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (750, 1142, 2662) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6486) [M::mem_pestat] mean and std.dev: (1807.40, 1490.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8398) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 949.005 CPU sec, 54.667 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 666908, 41, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (320, 398, 1278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3194) [M::mem_pestat] mean and std.dev: (647.15, 489.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4152) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (370, 426, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (130, 730) [M::mem_pestat] mean and std.dev: (431.97, 90.09) [M::mem_pestat] low and high boundaries for proper pairs: (10, 850) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 188, 1954) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5802) [M::mem_pestat] mean and std.dev: (1066.28, 1542.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7726) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 948.644 CPU sec, 42.296 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 666151, 37, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (639, 864, 1180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2262) [M::mem_pestat] mean and std.dev: (840.00, 436.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2803) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.19, 89.99) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 46, 455) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1305) [M::mem_pestat] mean and std.dev: (102.45, 152.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1730) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (894, 1566, 2367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5313) [M::mem_pestat] mean and std.dev: (1799.64, 1159.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6786) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 947.340 CPU sec, 54.782 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 666440, 36, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (351, 869, 2634) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7200) [M::mem_pestat] mean and std.dev: (1209.06, 1084.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9483) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.15, 89.90) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 48, 1100) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3264) [M::mem_pestat] mean and std.dev: (414.06, 620.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4346) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (760, 2294, 2920) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7240) [M::mem_pestat] mean and std.dev: (1804.20, 1168.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9400) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 946.412 CPU sec, 42.028 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 666867, 44, 10) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.17, 89.86) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 141, 1951) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5803) [M::mem_pestat] mean and std.dev: (1155.26, 1617.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7729) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (541, 1296, 1386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3076) [M::mem_pestat] mean and std.dev: (1024.67, 721.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3921) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 948.670 CPU sec, 55.772 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 667515, 34, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (297, 1819, 2065) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5601) [M::mem_pestat] mean and std.dev: (1071.12, 759.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7369) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.30, 90.17) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 56, 1792) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5348) [M::mem_pestat] mean and std.dev: (766.94, 1366.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7126) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 941.446 CPU sec, 41.882 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 666788, 39, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (361, 475, 1283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3127) [M::mem_pestat] mean and std.dev: (593.86, 333.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4049) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.11, 89.92) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 331, 2644) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7866) [M::mem_pestat] mean and std.dev: (1423.47, 1986.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10477) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1042, 1118, 2007) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3937) [M::mem_pestat] mean and std.dev: (1330.91, 572.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4902) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 944.624 CPU sec, 54.942 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 667154, 49, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (395, 449, 2120) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5570) [M::mem_pestat] mean and std.dev: (793.30, 787.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7295) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.21, 90.09) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 52, 2421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7217) [M::mem_pestat] mean and std.dev: (940.55, 1567.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9615) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1359, 1911, 3284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7134) [M::mem_pestat] mean and std.dev: (1938.08, 1265.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9059) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 941.713 CPU sec, 41.875 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 666153, 42, 12) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.13, 89.90) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 366, 2226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6640) [M::mem_pestat] mean and std.dev: (1405.26, 1747.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8847) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1034, 1247, 3054) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7094) [M::mem_pestat] mean and std.dev: (1523.91, 948.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9114) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 946.701 CPU sec, 54.907 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 666424, 44, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (281, 384, 1167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2939) [M::mem_pestat] mean and std.dev: (474.60, 334.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3825) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.28, 89.95) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 87, 1893) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5621) [M::mem_pestat] mean and std.dev: (988.90, 1514.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7485) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 940.805 CPU sec, 41.741 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 666153, 31, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (424, 740, 796) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1540) [M::mem_pestat] mean and std.dev: (511.75, 249.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1912) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.18, 90.00) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 174, 4353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13031) [M::mem_pestat] mean and std.dev: (1850.13, 2410.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17370) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 952.231 CPU sec, 54.874 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 666611, 38, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (335, 453, 1263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3119) [M::mem_pestat] mean and std.dev: (619.58, 402.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4047) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (431.99, 89.83) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 79, 3703) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11065) [M::mem_pestat] mean and std.dev: (1478.45, 2099.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14746) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (707, 1300, 4538) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12200) [M::mem_pestat] mean and std.dev: (2305.23, 2218.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16031) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 942.706 CPU sec, 41.872 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 666656, 46, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (292, 932, 1173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2935) [M::mem_pestat] mean and std.dev: (687.89, 430.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3816) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.17, 90.05) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 341, 2617) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7781) [M::mem_pestat] mean and std.dev: (1089.30, 1502.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10363) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (421, 1209, 2254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5920) [M::mem_pestat] mean and std.dev: (1481.29, 1332.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7753) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 951.228 CPU sec, 55.333 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 667205, 33, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (160, 276, 1206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3298) [M::mem_pestat] mean and std.dev: (427.30, 449.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4344) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.20, 89.82) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 80, 4349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12995) [M::mem_pestat] mean and std.dev: (1692.12, 2500.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17318) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 944.496 CPU sec, 41.867 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 666691, 46, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (412, 1151, 1824) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4648) [M::mem_pestat] mean and std.dev: (1356.92, 1040.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6060) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.23, 89.99) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 31, 1393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4139) [M::mem_pestat] mean and std.dev: (413.95, 803.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5512) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (886, 1236, 2451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5581) [M::mem_pestat] mean and std.dev: (1253.27, 836.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7146) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 946.963 CPU sec, 54.979 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 666926, 34, 11) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.18, 89.88) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 54, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 537) [M::mem_pestat] mean and std.dev: (45.08, 49.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 711) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1196, 1939, 2320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4568) [M::mem_pestat] mean and std.dev: (1664.64, 697.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5692) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 947.608 CPU sec, 42.323 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 666075, 30, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (368, 512, 1984) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5216) [M::mem_pestat] mean and std.dev: (1068.69, 1092.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6832) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.21, 89.87) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 69, 1263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3761) [M::mem_pestat] mean and std.dev: (434.65, 930.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5010) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (870, 1261, 2421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5523) [M::mem_pestat] mean and std.dev: (1686.50, 1341.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7074) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 953.127 CPU sec, 55.270 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 666970, 34, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (322, 1020, 1608) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4180) [M::mem_pestat] mean and std.dev: (961.83, 726.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5466) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.00, 89.94) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 731, 2784) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8316) [M::mem_pestat] mean and std.dev: (1383.32, 1948.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11082) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 944.107 CPU sec, 42.028 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 667066, 52, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (291, 562, 1138) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2832) [M::mem_pestat] mean and std.dev: (560.50, 443.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3679) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.15, 90.01) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 156, 2602) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7760) [M::mem_pestat] mean and std.dev: (1288.81, 2103.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10339) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 943.801 CPU sec, 54.336 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 666428, 39, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (372, 536, 1359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3333) [M::mem_pestat] mean and std.dev: (710.50, 466.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4320) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.14, 89.92) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 135, 2873) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8573) [M::mem_pestat] mean and std.dev: (1467.26, 2247.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11423) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 947.102 CPU sec, 42.051 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 666318, 37, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (343, 446, 1847) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4855) [M::mem_pestat] mean and std.dev: (697.25, 596.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6359) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.12, 90.01) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 40, 2386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7120) [M::mem_pestat] mean and std.dev: (746.91, 1357.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9487) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1159, 1328, 2167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4183) [M::mem_pestat] mean and std.dev: (1473.40, 881.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5191) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 952.396 CPU sec, 55.519 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 666810, 42, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.12, 90.05) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 92, 1098) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3242) [M::mem_pestat] mean and std.dev: (321.34, 622.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4314) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 941.719 CPU sec, 41.643 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 666074, 41, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (357, 985, 1585) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4041) [M::mem_pestat] mean and std.dev: (1007.00, 984.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5269) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.06, 89.91) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 79, 3638) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10874) [M::mem_pestat] mean and std.dev: (1581.10, 2155.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14492) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1762, 2134, 5184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12028) [M::mem_pestat] mean and std.dev: (3093.83, 2205.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15450) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 949.650 CPU sec, 54.901 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 666708, 42, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (282, 1543, 3717) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10587) [M::mem_pestat] mean and std.dev: (1985.60, 1964.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14022) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.23, 90.17) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (11, 48, 2734) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8180) [M::mem_pestat] mean and std.dev: (1248.34, 2062.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10903) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 945.450 CPU sec, 42.132 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 666871, 39, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (278, 734, 1380) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3584) [M::mem_pestat] mean and std.dev: (701.31, 466.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4686) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.06, 89.91) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 48, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 577) [M::mem_pestat] mean and std.dev: (73.38, 103.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 762) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (665, 986, 1856) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4238) [M::mem_pestat] mean and std.dev: (1216.40, 970.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5429) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 946.052 CPU sec, 53.880 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 666262, 44, 13) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.11, 90.02) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 159, 1895) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5651) [M::mem_pestat] mean and std.dev: (796.51, 1285.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7529) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (576, 1041, 2375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5973) [M::mem_pestat] mean and std.dev: (1155.67, 787.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7772) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 945.198 CPU sec, 41.961 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 666589, 37, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (294, 894, 2247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6153) [M::mem_pestat] mean and std.dev: (1228.94, 1297.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8106) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.26, 89.97) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 56, 1350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3998) [M::mem_pestat] mean and std.dev: (319.41, 632.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5322) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (896, 967, 2220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4868) [M::mem_pestat] mean and std.dev: (1187.82, 756.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6192) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 948.955 CPU sec, 55.613 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 667600, 37, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.29, 90.25) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 64, 2591) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7727) [M::mem_pestat] mean and std.dev: (932.79, 1430.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10295) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 942.503 CPU sec, 41.747 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 666766, 36, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (476, 1666, 3570) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9758) [M::mem_pestat] mean and std.dev: (2088.56, 1990.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12852) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (431.92, 89.82) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 33, 1666) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4968) [M::mem_pestat] mean and std.dev: (870.03, 1475.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6773) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 952.259 CPU sec, 54.237 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 666463, 30, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (268, 837, 1751) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4717) [M::mem_pestat] mean and std.dev: (1213.67, 1137.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6200) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.08, 89.97) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 238, 3282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9808) [M::mem_pestat] mean and std.dev: (2084.73, 3026.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14192) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1134, 1908, 2630) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5622) [M::mem_pestat] mean and std.dev: (1683.25, 666.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7118) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 945.781 CPU sec, 42.063 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 666907, 40, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (287, 701, 1948) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5270) [M::mem_pestat] mean and std.dev: (1181.15, 1329.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6931) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.30, 90.07) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 63, 2109) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6281) [M::mem_pestat] mean and std.dev: (903.43, 1260.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8367) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (471, 1244, 1507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3579) [M::mem_pestat] mean and std.dev: (948.46, 536.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4615) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 943.613 CPU sec, 53.843 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 666967, 37, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (369, 529, 2465) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6657) [M::mem_pestat] mean and std.dev: (1276.46, 1514.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8753) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (370, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (128, 733) [M::mem_pestat] mean and std.dev: (432.21, 90.24) [M::mem_pestat] low and high boundaries for proper pairs: (7, 854) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 134, 3043) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9069) [M::mem_pestat] mean and std.dev: (1708.38, 2468.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12082) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (885, 1377, 1982) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4176) [M::mem_pestat] mean and std.dev: (1342.56, 632.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5273) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 943.840 CPU sec, 41.954 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 666658, 40, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (529, 878, 1932) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4738) [M::mem_pestat] mean and std.dev: (1062.45, 936.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6141) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (370, 426, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (130, 730) [M::mem_pestat] mean and std.dev: (431.94, 90.07) [M::mem_pestat] low and high boundaries for proper pairs: (10, 850) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 313, 2076) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6172) [M::mem_pestat] mean and std.dev: (983.08, 1356.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8220) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1219, 1430, 2065) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3757) [M::mem_pestat] mean and std.dev: (1356.91, 533.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4603) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 946.226 CPU sec, 53.902 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 666835, 40, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (435, 994, 1510) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3660) [M::mem_pestat] mean and std.dev: (894.14, 609.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4735) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.27, 90.14) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 59, 1063) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3137) [M::mem_pestat] mean and std.dev: (457.59, 829.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4174) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1552, 1899, 2125) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (406, 3271) [M::mem_pestat] mean and std.dev: (1785.27, 626.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4292) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 939.324 CPU sec, 41.757 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 666382, 41, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (297, 432, 1064) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2598) [M::mem_pestat] mean and std.dev: (661.15, 472.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3365) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.36, 90.08) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 121, 798) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2352) [M::mem_pestat] mean and std.dev: (331.27, 574.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3129) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 952.418 CPU sec, 56.317 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 667161, 40, 11) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.25, 89.88) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 37, 461) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1357) [M::mem_pestat] mean and std.dev: (129.06, 240.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1805) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (930, 1276, 1870) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3750) [M::mem_pestat] mean and std.dev: (1087.33, 495.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4690) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 945.598 CPU sec, 42.185 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666967, 46, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (316, 896, 1047) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2509) [M::mem_pestat] mean and std.dev: (705.82, 428.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3240) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (370, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (130, 730) [M::mem_pestat] mean and std.dev: (431.95, 90.08) [M::mem_pestat] low and high boundaries for proper pairs: (10, 850) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 59, 2715) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8115) [M::mem_pestat] mean and std.dev: (1308.00, 1936.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10815) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 943.156 CPU sec, 54.059 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666524, 37, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (289, 470, 525) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 997) [M::mem_pestat] mean and std.dev: (402.30, 161.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1233) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (431.97, 89.97) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 64, 1555) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4605) [M::mem_pestat] mean and std.dev: (717.39, 1174.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6130) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1112, 1456, 2008) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3800) [M::mem_pestat] mean and std.dev: (1514.78, 557.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4696) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 942.170 CPU sec, 41.780 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 666864, 39, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (332, 494, 1423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3605) [M::mem_pestat] mean and std.dev: (677.58, 451.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4696) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (370, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (130, 730) [M::mem_pestat] mean and std.dev: (432.09, 90.02) [M::mem_pestat] low and high boundaries for proper pairs: (10, 850) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 35, 1431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4259) [M::mem_pestat] mean and std.dev: (563.97, 932.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5673) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (487, 1753, 2700) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7126) [M::mem_pestat] mean and std.dev: (1568.89, 1221.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9339) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 945.351 CPU sec, 53.620 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 666710, 40, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (329, 513, 1243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3071) [M::mem_pestat] mean and std.dev: (791.35, 704.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3985) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.18, 90.05) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 37, 1408) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4190) [M::mem_pestat] mean and std.dev: (425.37, 866.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5581) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 944.587 CPU sec, 41.966 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 666821, 29, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (311, 469, 1191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2951) [M::mem_pestat] mean and std.dev: (737.23, 618.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3831) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.05, 89.93) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 63, 2029) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6039) [M::mem_pestat] mean and std.dev: (801.75, 1375.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8044) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1213, 1791, 2921) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6337) [M::mem_pestat] mean and std.dev: (1975.73, 1162.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8045) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 946.257 CPU sec, 55.745 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 667047, 48, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (275, 403, 2060) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5630) [M::mem_pestat] mean and std.dev: (901.77, 843.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7415) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.35, 90.14) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 121, 3567) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10647) [M::mem_pestat] mean and std.dev: (1543.21, 2109.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14187) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (552, 1546, 3234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8598) [M::mem_pestat] mean and std.dev: (2395.30, 2377.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11906) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 940.126 CPU sec, 41.771 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 666927, 37, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (263, 445, 926) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2252) [M::mem_pestat] mean and std.dev: (395.36, 236.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2915) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.13, 89.91) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 101, 3021) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9009) [M::mem_pestat] mean and std.dev: (1324.32, 1987.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12003) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (792, 1254, 2197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5007) [M::mem_pestat] mean and std.dev: (1128.90, 758.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6412) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 947.713 CPU sec, 55.859 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 666776, 38, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (222, 1164, 1844) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5088) [M::mem_pestat] mean and std.dev: (873.22, 730.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6710) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.23, 89.82) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 38, 2867) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8569) [M::mem_pestat] mean and std.dev: (1456.35, 2321.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11420) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (336, 1112, 2059) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5505) [M::mem_pestat] mean and std.dev: (969.92, 651.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7228) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 943.731 CPU sec, 42.155 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 666758, 44, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (804, 928, 1796) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3780) [M::mem_pestat] mean and std.dev: (945.40, 480.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4772) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.34, 90.29) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 68, 1910) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5684) [M::mem_pestat] mean and std.dev: (775.39, 1268.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7571) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 946.014 CPU sec, 54.572 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 667002, 42, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (305, 440, 4029) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11477) [M::mem_pestat] mean and std.dev: (1793.53, 2461.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15201) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.43, 90.04) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 309, 2583) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7703) [M::mem_pestat] mean and std.dev: (1178.33, 1610.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10263) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (784, 1238, 2906) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7150) [M::mem_pestat] mean and std.dev: (1859.27, 1171.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9272) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 947.782 CPU sec, 42.054 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 666635, 35, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (421, 937, 1718) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4312) [M::mem_pestat] mean and std.dev: (1029.25, 728.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5609) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.14, 90.07) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 88, 1404) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4162) [M::mem_pestat] mean and std.dev: (247.50, 455.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5541) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (716, 1644, 2899) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7265) [M::mem_pestat] mean and std.dev: (1911.38, 1857.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9448) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 943.718 CPU sec, 53.534 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 666860, 47, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (383, 1708, 1853) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4793) [M::mem_pestat] mean and std.dev: (1116.62, 658.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6263) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.14, 90.05) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 72, 1981) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5883) [M::mem_pestat] mean and std.dev: (1036.53, 1461.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7834) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 946.806 CPU sec, 41.994 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 666979, 33, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (171, 507, 583) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1407) [M::mem_pestat] mean and std.dev: (322.12, 178.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1819) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.25, 90.03) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 309, 3181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9475) [M::mem_pestat] mean and std.dev: (1825.18, 2570.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12622) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 944.806 CPU sec, 53.975 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 667320, 33, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (566, 1261, 1880) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4508) [M::mem_pestat] mean and std.dev: (1197.39, 783.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5822) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.01, 89.90) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (41, 164, 3440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10238) [M::mem_pestat] mean and std.dev: (1849.76, 2470.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13637) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1159, 1261, 1370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (737, 1792) [M::mem_pestat] mean and std.dev: (1251.50, 215.44) [M::mem_pestat] low and high boundaries for proper pairs: (390, 2113) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 942.074 CPU sec, 54.014 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 666969, 33, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (403, 1493, 3230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8884) [M::mem_pestat] mean and std.dev: (2327.69, 2616.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12793) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.08, 89.85) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (49, 1856, 4047) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12043) [M::mem_pestat] mean and std.dev: (2001.76, 1833.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16041) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (908, 1263, 5907) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15905) [M::mem_pestat] mean and std.dev: (2522.44, 2672.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20904) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 943.680 CPU sec, 41.922 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 666907, 43, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (352, 1591, 2025) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5371) [M::mem_pestat] mean and std.dev: (1146.64, 772.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7044) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.17, 90.00) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 55, 1682) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4994) [M::mem_pestat] mean and std.dev: (751.20, 1318.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6650) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (981, 1304, 3101) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7341) [M::mem_pestat] mean and std.dev: (1514.00, 1036.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9461) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 945.019 CPU sec, 54.744 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 666906, 44, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (301, 781, 1206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3016) [M::mem_pestat] mean and std.dev: (698.12, 454.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3921) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.32, 90.02) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 143, 2908) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8684) [M::mem_pestat] mean and std.dev: (1443.98, 2138.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11572) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 938.979 CPU sec, 41.776 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 666876, 45, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (319, 944, 1613) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4201) [M::mem_pestat] mean and std.dev: (1160.18, 1050.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5495) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (370, 426, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (130, 730) [M::mem_pestat] mean and std.dev: (432.02, 90.16) [M::mem_pestat] low and high boundaries for proper pairs: (10, 850) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 46, 1459) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4327) [M::mem_pestat] mean and std.dev: (623.80, 1016.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5761) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (778, 1211, 1682) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3490) [M::mem_pestat] mean and std.dev: (1185.38, 677.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4394) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 947.469 CPU sec, 54.611 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 666517, 42, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (303, 749, 1675) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4419) [M::mem_pestat] mean and std.dev: (1043.75, 943.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5791) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.32, 90.08) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 300, 2066) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6136) [M::mem_pestat] mean and std.dev: (1042.17, 1367.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8171) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 943.599 CPU sec, 41.881 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666686, 47, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (610, 1091, 1906) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4498) [M::mem_pestat] mean and std.dev: (1237.82, 786.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5794) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (370, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (130, 730) [M::mem_pestat] mean and std.dev: (431.98, 90.00) [M::mem_pestat] low and high boundaries for proper pairs: (10, 850) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 57, 1972) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5864) [M::mem_pestat] mean and std.dev: (858.09, 1318.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7810) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (941, 1236, 2053) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4277) [M::mem_pestat] mean and std.dev: (1436.00, 1072.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5725) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 945.324 CPU sec, 55.186 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 666224, 42, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (359, 948, 2366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6380) [M::mem_pestat] mean and std.dev: (1201.53, 1323.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8387) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.01, 89.84) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 147, 2642) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7876) [M::mem_pestat] mean and std.dev: (933.15, 1526.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10493) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 948.958 CPU sec, 42.348 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 667049, 46, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (281, 386, 649) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1385) [M::mem_pestat] mean and std.dev: (418.21, 243.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1753) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.31, 90.25) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 46, 1655) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4941) [M::mem_pestat] mean and std.dev: (817.20, 1375.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6584) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 946.094 CPU sec, 54.225 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 667246, 32, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (432, 871, 1426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3414) [M::mem_pestat] mean and std.dev: (823.00, 686.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4408) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.28, 90.20) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 107, 3228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9632) [M::mem_pestat] mean and std.dev: (1605.12, 2408.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12834) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 941.180 CPU sec, 41.861 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 666197, 38, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (369, 492, 1768) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4566) [M::mem_pestat] mean and std.dev: (1172.23, 1122.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5965) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.01, 89.82) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 37, 385) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1129) [M::mem_pestat] mean and std.dev: (53.93, 82.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1501) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1114, 1423, 1888) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3436) [M::mem_pestat] mean and std.dev: (1407.30, 672.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4210) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 948.133 CPU sec, 54.407 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 666512, 34, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (262, 365, 408) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700) [M::mem_pestat] mean and std.dev: (287.88, 114.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 846) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.23, 89.93) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 132, 2568) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7650) [M::mem_pestat] mean and std.dev: (975.39, 1341.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10191) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (743, 1053, 1927) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4295) [M::mem_pestat] mean and std.dev: (1255.82, 922.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5479) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 946.956 CPU sec, 42.139 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 666955, 34, 13) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (370, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (130, 730) [M::mem_pestat] mean and std.dev: (432.04, 90.04) [M::mem_pestat] low and high boundaries for proper pairs: (10, 850) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (11, 61, 1324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3950) [M::mem_pestat] mean and std.dev: (529.19, 961.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5263) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (807, 1031, 2742) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6612) [M::mem_pestat] mean and std.dev: (1528.23, 1257.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8547) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 952.799 CPU sec, 54.735 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 666854, 34, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (474, 1146, 2154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5514) [M::mem_pestat] mean and std.dev: (1289.86, 966.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7194) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (370, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (130, 730) [M::mem_pestat] mean and std.dev: (432.17, 90.12) [M::mem_pestat] low and high boundaries for proper pairs: (10, 850) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 101, 2444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7276) [M::mem_pestat] mean and std.dev: (1196.09, 1854.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9692) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (293, 448, 1226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3092) [M::mem_pestat] mean and std.dev: (681.30, 506.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4025) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 948.375 CPU sec, 42.159 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 667235, 41, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (345, 418, 1927) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5091) [M::mem_pestat] mean and std.dev: (1106.86, 1232.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6673) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.16, 89.98) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 180, 2547) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7615) [M::mem_pestat] mean and std.dev: (1025.64, 1640.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10149) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 947.645 CPU sec, 55.606 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 666151, 44, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (300, 514, 1973) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5319) [M::mem_pestat] mean and std.dev: (796.56, 779.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6992) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.09, 89.82) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 209, 3055) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9103) [M::mem_pestat] mean and std.dev: (1495.95, 2118.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12127) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (941, 1496, 2454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5480) [M::mem_pestat] mean and std.dev: (1588.00, 861.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6993) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 941.195 CPU sec, 41.998 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 667054, 36, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (376, 610, 1430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3538) [M::mem_pestat] mean and std.dev: (808.30, 585.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4592) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (370, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (130, 730) [M::mem_pestat] mean and std.dev: (432.00, 90.02) [M::mem_pestat] low and high boundaries for proper pairs: (10, 850) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 1227, 4386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13112) [M::mem_pestat] mean and std.dev: (2247.67, 2637.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17475) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (608, 1286, 3693) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9863) [M::mem_pestat] mean and std.dev: (1835.73, 1407.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12948) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 946.719 CPU sec, 55.010 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 666966, 40, 13) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.23, 89.80) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 45, 555) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1631) [M::mem_pestat] mean and std.dev: (83.58, 136.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2169) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (869, 1028, 1948) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4106) [M::mem_pestat] mean and std.dev: (1050.42, 678.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5185) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 939.947 CPU sec, 41.785 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 667048, 34, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (316, 362, 1198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2962) [M::mem_pestat] mean and std.dev: (691.25, 739.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3844) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.32, 89.87) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 85, 2592) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7732) [M::mem_pestat] mean and std.dev: (1682.00, 2440.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11443) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 941.436 CPU sec, 53.876 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 666466, 39, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (271, 638, 1696) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4546) [M::mem_pestat] mean and std.dev: (952.69, 876.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5971) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (370, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (130, 730) [M::mem_pestat] mean and std.dev: (432.11, 89.99) [M::mem_pestat] low and high boundaries for proper pairs: (10, 850) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 179, 3943) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11781) [M::mem_pestat] mean and std.dev: (1911.90, 2624.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15700) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (784, 1483, 2423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5701) [M::mem_pestat] mean and std.dev: (1474.75, 942.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7340) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 945.598 CPU sec, 42.108 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 666508, 38, 10) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.08, 89.87) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 278, 2381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7085) [M::mem_pestat] mean and std.dev: (1188.76, 1581.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9437) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (913, 1974, 4006) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10192) [M::mem_pestat] mean and std.dev: (2694.20, 2386.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13285) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 944.592 CPU sec, 54.136 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 667435, 42, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (298, 442, 1301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3307) [M::mem_pestat] mean and std.dev: (697.23, 617.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4310) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.22, 89.99) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 97, 2585) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7715) [M::mem_pestat] mean and std.dev: (1378.24, 1875.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10280) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 944.177 CPU sec, 42.115 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 666957, 43, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (389, 554, 1038) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2336) [M::mem_pestat] mean and std.dev: (649.08, 417.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2985) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.11, 89.90) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 49, 1851) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5507) [M::mem_pestat] mean and std.dev: (756.41, 1248.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7335) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (797, 1811, 3242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8132) [M::mem_pestat] mean and std.dev: (1865.83, 1657.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10577) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 950.970 CPU sec, 54.566 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 666762, 47, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (470, 832, 1591) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3833) [M::mem_pestat] mean and std.dev: (854.92, 489.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4954) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.27, 90.05) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 54, 1578) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4700) [M::mem_pestat] mean and std.dev: (940.60, 1437.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6690) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (781, 1157, 1755) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3703) [M::mem_pestat] mean and std.dev: (1170.23, 679.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4677) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 942.947 CPU sec, 41.732 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666683, 36, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (485, 647, 1490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3500) [M::mem_pestat] mean and std.dev: (795.82, 572.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4505) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (370, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (128, 733) [M::mem_pestat] mean and std.dev: (432.16, 90.33) [M::mem_pestat] low and high boundaries for proper pairs: (7, 854) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 45, 857) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2525) [M::mem_pestat] mean and std.dev: (263.39, 528.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3359) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1114, 1636, 3176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7300) [M::mem_pestat] mean and std.dev: (1765.80, 1028.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9362) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 949.664 CPU sec, 53.889 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 666335, 38, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (370, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (130, 730) [M::mem_pestat] mean and std.dev: (431.98, 90.07) [M::mem_pestat] low and high boundaries for proper pairs: (10, 850) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 57, 2237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6675) [M::mem_pestat] mean and std.dev: (1135.18, 1662.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8894) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 951.839 CPU sec, 42.443 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 667333, 46, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (354, 387, 2675) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7317) [M::mem_pestat] mean and std.dev: (1205.92, 1557.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9638) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.29, 90.22) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 56, 2405) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7169) [M::mem_pestat] mean and std.dev: (977.18, 1695.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9551) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (778, 1053, 2834) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6946) [M::mem_pestat] mean and std.dev: (1526.85, 1060.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9002) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 944.655 CPU sec, 54.603 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 666768, 35, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (466, 1136, 1707) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4189) [M::mem_pestat] mean and std.dev: (973.13, 672.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5430) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (370, 426, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (130, 730) [M::mem_pestat] mean and std.dev: (431.98, 90.15) [M::mem_pestat] low and high boundaries for proper pairs: (10, 850) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 85, 2719) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8125) [M::mem_pestat] mean and std.dev: (1153.82, 1583.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10828) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 948.291 CPU sec, 42.290 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666360, 38, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (643, 760, 1345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2749) [M::mem_pestat] mean and std.dev: (880.00, 540.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3451) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.10, 90.00) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 254, 2897) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8645) [M::mem_pestat] mean and std.dev: (1406.81, 1803.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11519) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 944.883 CPU sec, 55.103 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 666677, 38, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (420, 493, 1082) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2406) [M::mem_pestat] mean and std.dev: (808.87, 674.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3505) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.08, 89.79) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 86, 1822) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5412) [M::mem_pestat] mean and std.dev: (998.55, 1547.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7207) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (640, 729, 2699) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6817) [M::mem_pestat] mean and std.dev: (1419.18, 1516.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8876) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 947.324 CPU sec, 42.137 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 666886, 35, 14) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.15, 89.97) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 54, 1891) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5619) [M::mem_pestat] mean and std.dev: (692.87, 1176.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7483) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (622, 1340, 2167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5257) [M::mem_pestat] mean and std.dev: (1316.85, 799.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6802) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 947.789 CPU sec, 54.791 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 667567, 35, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (375, 662, 1530) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3840) [M::mem_pestat] mean and std.dev: (1000.18, 1018.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5073) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.01, 89.84) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 153, 1499) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4455) [M::mem_pestat] mean and std.dev: (784.16, 1181.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5933) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (557, 904, 2009) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4913) [M::mem_pestat] mean and std.dev: (1178.31, 887.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6365) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 945.472 CPU sec, 42.105 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 666676, 40, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (375, 594, 1095) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2535) [M::mem_pestat] mean and std.dev: (791.71, 592.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3255) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.20, 89.92) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 64, 2749) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8211) [M::mem_pestat] mean and std.dev: (1170.67, 1731.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10942) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 949.435 CPU sec, 54.581 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 666097, 48, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (342, 515, 1924) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5088) [M::mem_pestat] mean and std.dev: (778.08, 662.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6670) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (370, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (130, 730) [M::mem_pestat] mean and std.dev: (432.09, 90.16) [M::mem_pestat] low and high boundaries for proper pairs: (10, 850) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 93, 1282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3804) [M::mem_pestat] mean and std.dev: (601.42, 959.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5065) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (654, 1069, 2006) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4710) [M::mem_pestat] mean and std.dev: (1397.00, 1062.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6062) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 945.284 CPU sec, 42.152 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 667607, 42, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1011, 1154, 1896) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3666) [M::mem_pestat] mean and std.dev: (1188.38, 866.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4654) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (370, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (130, 730) [M::mem_pestat] mean and std.dev: (432.04, 90.00) [M::mem_pestat] low and high boundaries for proper pairs: (10, 850) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (38, 83, 2896) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8612) [M::mem_pestat] mean and std.dev: (1471.05, 2370.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11470) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (619, 1024, 1443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3091) [M::mem_pestat] mean and std.dev: (807.33, 400.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3915) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 943.691 CPU sec, 53.811 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 666739, 43, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (203, 494, 1366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3692) [M::mem_pestat] mean and std.dev: (655.69, 530.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4855) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (370, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (130, 730) [M::mem_pestat] mean and std.dev: (432.02, 90.08) [M::mem_pestat] low and high boundaries for proper pairs: (10, 850) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (41, 751, 4329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12905) [M::mem_pestat] mean and std.dev: (2343.58, 2805.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17193) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (983, 1143, 3743) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9263) [M::mem_pestat] mean and std.dev: (2348.33, 2114.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12023) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 948.437 CPU sec, 42.130 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 666376, 37, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (190, 349, 1792) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4996) [M::mem_pestat] mean and std.dev: (743.27, 708.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6598) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.10, 89.94) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 161, 2721) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8121) [M::mem_pestat] mean and std.dev: (1406.72, 1949.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10821) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 948.503 CPU sec, 55.389 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 666957, 32, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (335, 871, 1649) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4277) [M::mem_pestat] mean and std.dev: (961.94, 737.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5591) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.20, 89.95) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 95, 1383) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4097) [M::mem_pestat] mean and std.dev: (519.10, 964.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5454) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 950.947 CPU sec, 42.298 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 666570, 40, 10) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.09, 89.95) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 141, 3659) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10939) [M::mem_pestat] mean and std.dev: (2106.05, 2949.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14579) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (812, 1079, 1647) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3317) [M::mem_pestat] mean and std.dev: (1196.00, 609.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4152) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 942.913 CPU sec, 54.309 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 667257, 35, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (254, 403, 494) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 974) [M::mem_pestat] mean and std.dev: (319.00, 115.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1214) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.30, 90.00) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 208, 4326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12918) [M::mem_pestat] mean and std.dev: (1831.60, 2324.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17214) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 946.654 CPU sec, 42.042 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 667169, 39, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (210, 332, 1244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3312) [M::mem_pestat] mean and std.dev: (770.15, 673.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4346) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.05, 90.06) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 184, 2449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7309) [M::mem_pestat] mean and std.dev: (1280.13, 1655.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9739) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 947.788 CPU sec, 55.177 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 667050, 34, 15) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (370, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (130, 730) [M::mem_pestat] mean and std.dev: (432.14, 90.10) [M::mem_pestat] low and high boundaries for proper pairs: (10, 850) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 75, 2759) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8227) [M::mem_pestat] mean and std.dev: (1205.19, 1852.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10961) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (738, 1029, 1610) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3354) [M::mem_pestat] mean and std.dev: (1160.29, 680.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4226) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 945.082 CPU sec, 42.085 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 667507, 46, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (258, 636, 1736) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4692) [M::mem_pestat] mean and std.dev: (1028.00, 1161.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6170) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (370, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (130, 730) [M::mem_pestat] mean and std.dev: (432.09, 89.95) [M::mem_pestat] low and high boundaries for proper pairs: (10, 850) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 90, 1139) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3359) [M::mem_pestat] mean and std.dev: (420.70, 740.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4469) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (502, 1225, 1699) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4093) [M::mem_pestat] mean and std.dev: (1048.08, 565.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5290) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 947.482 CPU sec, 53.824 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 666986, 41, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (286, 490, 1639) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4345) [M::mem_pestat] mean and std.dev: (992.75, 962.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5698) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (370, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (130, 730) [M::mem_pestat] mean and std.dev: (432.08, 90.06) [M::mem_pestat] low and high boundaries for proper pairs: (10, 850) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 49, 1457) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4331) [M::mem_pestat] mean and std.dev: (468.59, 937.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5768) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (268, 1830, 2939) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8281) [M::mem_pestat] mean and std.dev: (1723.36, 1447.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10952) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 951.827 CPU sec, 42.291 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 666489, 41, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.14, 89.90) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 122, 2387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7117) [M::mem_pestat] mean and std.dev: (1016.26, 1307.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9482) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 949.772 CPU sec, 54.494 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 667480, 36, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (380, 613, 1284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3092) [M::mem_pestat] mean and std.dev: (923.14, 669.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3996) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.08, 90.04) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 667, 2639) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7867) [M::mem_pestat] mean and std.dev: (1151.97, 1468.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10481) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (765, 1138, 1862) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4056) [M::mem_pestat] mean and std.dev: (1412.38, 1087.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5762) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 940.677 CPU sec, 41.885 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 666616, 56, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (766, 1040, 2751) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6721) [M::mem_pestat] mean and std.dev: (1715.70, 1602.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8706) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (370, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (128, 733) [M::mem_pestat] mean and std.dev: (432.20, 90.24) [M::mem_pestat] low and high boundaries for proper pairs: (7, 854) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 59, 1236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3662) [M::mem_pestat] mean and std.dev: (414.50, 751.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4875) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 940.582 CPU sec, 55.775 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 666420, 41, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (240, 349, 1084) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2772) [M::mem_pestat] mean and std.dev: (543.93, 408.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3616) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 426, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.00, 90.06) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 63, 1786) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5308) [M::mem_pestat] mean and std.dev: (731.66, 1288.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7069) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (749, 1043, 6784) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 18854) [M::mem_pestat] mean and std.dev: (2770.45, 3184.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 24889) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 949.577 CPU sec, 55.215 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666725, 33, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (420, 692, 2582) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6906) [M::mem_pestat] mean and std.dev: (1634.64, 1794.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9068) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.23, 89.98) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 61, 1427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4223) [M::mem_pestat] mean and std.dev: (511.57, 899.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5621) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 944.644 CPU sec, 42.159 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 666656, 40, 12) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.16, 90.05) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 265, 3256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9714) [M::mem_pestat] mean and std.dev: (1810.53, 2655.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12943) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (654, 1086, 2966) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7590) [M::mem_pestat] mean and std.dev: (1912.50, 2000.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9913) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 949.064 CPU sec, 55.152 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 666628, 40, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (198, 377, 862) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2190) [M::mem_pestat] mean and std.dev: (364.00, 260.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2854) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.20, 90.01) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 45, 2026) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6052) [M::mem_pestat] mean and std.dev: (1051.49, 1595.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8065) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 945.002 CPU sec, 42.152 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 667443, 35, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (356, 493, 1254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3050) [M::mem_pestat] mean and std.dev: (620.15, 443.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3948) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (370, 426, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (130, 730) [M::mem_pestat] mean and std.dev: (431.92, 89.97) [M::mem_pestat] low and high boundaries for proper pairs: (10, 850) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 43, 1943) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5783) [M::mem_pestat] mean and std.dev: (989.66, 1559.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7703) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (666, 1011, 1310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2598) [M::mem_pestat] mean and std.dev: (798.50, 364.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3242) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 944.556 CPU sec, 55.262 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 666364, 38, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (317, 790, 1983) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5315) [M::mem_pestat] mean and std.dev: (1040.62, 962.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6981) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.42, 89.78) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 51, 1503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4465) [M::mem_pestat] mean and std.dev: (976.68, 1526.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7083) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (857, 1520, 2197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4877) [M::mem_pestat] mean and std.dev: (1446.30, 977.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6217) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 944.168 CPU sec, 41.980 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 667547, 36, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (344, 512, 664) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1304) [M::mem_pestat] mean and std.dev: (458.58, 231.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1624) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.10, 89.98) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 65, 1945) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5779) [M::mem_pestat] mean and std.dev: (843.91, 1278.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7696) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 940.816 CPU sec, 55.171 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 666249, 39, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (318, 909, 1407) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3585) [M::mem_pestat] mean and std.dev: (873.42, 807.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4674) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.12, 89.90) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 136, 4352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12998) [M::mem_pestat] mean and std.dev: (1711.38, 2205.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17321) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (401, 1056, 1844) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4730) [M::mem_pestat] mean and std.dev: (1084.42, 828.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6173) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 945.495 CPU sec, 42.145 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 666892, 31, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (409, 1293, 3570) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9892) [M::mem_pestat] mean and std.dev: (1982.43, 1977.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13053) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.46, 90.16) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 62, 1421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4207) [M::mem_pestat] mean and std.dev: (325.81, 674.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5600) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 944.470 CPU sec, 54.703 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 666834, 43, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (200, 909, 1253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3359) [M::mem_pestat] mean and std.dev: (804.33, 623.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4412) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.21, 89.92) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 56, 1554) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4632) [M::mem_pestat] mean and std.dev: (696.59, 1278.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6171) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1026, 1106, 1735) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3153) [M::mem_pestat] mean and std.dev: (1210.91, 766.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4278) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 942.027 CPU sec, 41.795 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666873, 40, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (214, 422, 748) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1816) [M::mem_pestat] mean and std.dev: (329.44, 224.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2350) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.12, 90.10) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 41, 1259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3745) [M::mem_pestat] mean and std.dev: (480.50, 859.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4988) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (758, 1367, 2244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5216) [M::mem_pestat] mean and std.dev: (1512.59, 1117.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6702) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 946.347 CPU sec, 54.334 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 667203, 30, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (357, 757, 972) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2202) [M::mem_pestat] mean and std.dev: (663.55, 357.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2817) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.09, 89.89) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 179, 2811) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8363) [M::mem_pestat] mean and std.dev: (1347.47, 1748.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11139) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 948.753 CPU sec, 42.286 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 666988, 40, 10) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (370, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (128, 733) [M::mem_pestat] mean and std.dev: (432.12, 90.20) [M::mem_pestat] low and high boundaries for proper pairs: (7, 854) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 279, 3411) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10185) [M::mem_pestat] mean and std.dev: (1580.50, 2006.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13572) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1040, 1622, 1922) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3686) [M::mem_pestat] mean and std.dev: (1527.20, 942.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5296) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 946.665 CPU sec, 54.591 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 667088, 36, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (385, 477, 1501) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3733) [M::mem_pestat] mean and std.dev: (803.83, 625.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4849) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.13, 89.95) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 53, 2885) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8619) [M::mem_pestat] mean and std.dev: (1463.43, 2411.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11486) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 938.591 CPU sec, 41.868 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 666677, 39, 10) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.23, 90.19) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 61, 2118) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6300) [M::mem_pestat] mean and std.dev: (911.74, 1291.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8391) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (577, 1300, 1828) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4330) [M::mem_pestat] mean and std.dev: (1308.30, 915.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5581) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 947.229 CPU sec, 56.168 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666797, 38, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (356, 558, 632) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1184) [M::mem_pestat] mean and std.dev: (437.22, 151.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1460) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.13, 89.96) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 118, 1365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4061) [M::mem_pestat] mean and std.dev: (451.27, 745.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5409) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 946.337 CPU sec, 42.105 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 666891, 37, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (526, 1196, 1774) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4270) [M::mem_pestat] mean and std.dev: (1243.68, 865.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5518) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.08, 89.91) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (40, 165, 1424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4192) [M::mem_pestat] mean and std.dev: (519.12, 850.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5576) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (619, 968, 2772) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7078) [M::mem_pestat] mean and std.dev: (1267.00, 1237.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9231) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 948.524 CPU sec, 54.405 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 667283, 41, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (458, 586, 978) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2018) [M::mem_pestat] mean and std.dev: (599.33, 290.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2538) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.09, 89.92) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 92, 2370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7058) [M::mem_pestat] mean and std.dev: (1111.83, 1550.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9402) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1069, 1389, 1921) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3625) [M::mem_pestat] mean and std.dev: (1263.50, 652.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4477) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 944.270 CPU sec, 42.100 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 666738, 34, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (323, 791, 1406) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3572) [M::mem_pestat] mean and std.dev: (772.13, 677.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4655) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.00, 90.03) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (68, 2349, 4500) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13364) [M::mem_pestat] mean and std.dev: (2976.26, 3244.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17796) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 947.021 CPU sec, 54.967 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 666078, 45, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (468, 1303, 2084) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5316) [M::mem_pestat] mean and std.dev: (1545.20, 1363.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7001) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.16, 89.97) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 63, 1881) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5613) [M::mem_pestat] mean and std.dev: (853.43, 1277.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7479) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (341, 1313, 2403) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6527) [M::mem_pestat] mean and std.dev: (1395.27, 1005.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8589) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 944.339 CPU sec, 41.861 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 666757, 33, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (249, 538, 1078) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2736) [M::mem_pestat] mean and std.dev: (629.58, 449.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3565) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.34, 90.14) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 43, 2561) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7637) [M::mem_pestat] mean and std.dev: (972.67, 1751.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10175) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 945.257 CPU sec, 55.154 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 666701, 34, 15) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (370, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (130, 730) [M::mem_pestat] mean and std.dev: (432.07, 90.10) [M::mem_pestat] low and high boundaries for proper pairs: (10, 850) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 55, 2330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6948) [M::mem_pestat] mean and std.dev: (1104.73, 1708.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9257) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (785, 1410, 1949) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4277) [M::mem_pestat] mean and std.dev: (1470.80, 1036.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5615) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 948.953 CPU sec, 42.052 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 666681, 38, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (285, 977, 1610) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4260) [M::mem_pestat] mean and std.dev: (976.23, 988.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5585) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.08, 90.02) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 53, 2753) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8213) [M::mem_pestat] mean and std.dev: (1309.44, 1999.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10943) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (445, 1320, 3839) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10627) [M::mem_pestat] mean and std.dev: (2654.57, 2638.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14021) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 948.181 CPU sec, 54.084 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 667073, 45, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (821, 1378, 2638) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6272) [M::mem_pestat] mean and std.dev: (1911.94, 1638.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8467) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.17, 89.90) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (38, 403, 2983) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8873) [M::mem_pestat] mean and std.dev: (1570.82, 2210.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11818) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (986, 1514, 5880) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15668) [M::mem_pestat] mean and std.dev: (2793.09, 2756.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20562) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 941.081 CPU sec, 41.862 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 667563, 42, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (353, 1031, 1746) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4532) [M::mem_pestat] mean and std.dev: (1075.90, 844.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5925) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.08, 89.88) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 146, 2904) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8662) [M::mem_pestat] mean and std.dev: (1785.17, 2715.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12648) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (875, 1896, 1973) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4169) [M::mem_pestat] mean and std.dev: (1374.33, 669.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5267) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 946.649 CPU sec, 56.338 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666623, 45, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (423, 810, 1460) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3534) [M::mem_pestat] mean and std.dev: (754.33, 459.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4571) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.23, 90.01) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 178, 1736) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5164) [M::mem_pestat] mean and std.dev: (908.89, 1232.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6878) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1005, 1482, 3034) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7092) [M::mem_pestat] mean and std.dev: (1623.50, 1259.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9121) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 941.928 CPU sec, 41.874 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 667043, 49, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (337, 459, 1821) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4789) [M::mem_pestat] mean and std.dev: (817.29, 882.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6273) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.19, 89.92) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 38, 2082) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6212) [M::mem_pestat] mean and std.dev: (899.26, 1566.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8277) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 945.921 CPU sec, 55.314 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 666466, 47, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (296, 637, 1895) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5093) [M::mem_pestat] mean and std.dev: (868.82, 807.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6692) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.20, 89.89) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 104, 2147) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6403) [M::mem_pestat] mean and std.dev: (1316.15, 1868.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8789) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (304, 800, 2696) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7480) [M::mem_pestat] mean and std.dev: (1448.36, 1447.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9872) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 939.158 CPU sec, 41.836 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 666325, 43, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (372, 406, 1131) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2649) [M::mem_pestat] mean and std.dev: (701.00, 495.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3408) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.12, 89.98) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 38, 1266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3766) [M::mem_pestat] mean and std.dev: (398.56, 727.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5016) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (368, 865, 1116) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2612) [M::mem_pestat] mean and std.dev: (833.43, 521.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3360) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 945.099 CPU sec, 54.804 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 666541, 47, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.38, 90.07) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 75, 2640) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7874) [M::mem_pestat] mean and std.dev: (1277.47, 1770.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10491) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 948.927 CPU sec, 42.361 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 666909, 40, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (335, 639, 2027) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5411) [M::mem_pestat] mean and std.dev: (1125.19, 1272.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7103) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.20, 89.97) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 50, 781) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2301) [M::mem_pestat] mean and std.dev: (169.82, 380.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3061) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (982, 1884, 2460) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5416) [M::mem_pestat] mean and std.dev: (1726.56, 1001.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6894) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 946.007 CPU sec, 55.095 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 667008, 37, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (401, 797, 1166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2696) [M::mem_pestat] mean and std.dev: (775.61, 492.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3461) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (370, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (128, 733) [M::mem_pestat] mean and std.dev: (432.11, 90.14) [M::mem_pestat] low and high boundaries for proper pairs: (7, 854) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 39, 130) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 360) [M::mem_pestat] mean and std.dev: (36.10, 34.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 475) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 944.315 CPU sec, 41.985 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 667159, 43, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (278, 707, 2396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6632) [M::mem_pestat] mean and std.dev: (1278.33, 1555.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8750) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.24, 89.97) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 59, 2933) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8737) [M::mem_pestat] mean and std.dev: (1272.49, 1872.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11639) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (452, 1094, 2003) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5105) [M::mem_pestat] mean and std.dev: (1192.08, 906.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6656) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 944.996 CPU sec, 54.756 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666725, 32, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (392, 892, 3174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8738) [M::mem_pestat] mean and std.dev: (1450.36, 1434.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11520) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.19, 89.98) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 389, 3033) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9055) [M::mem_pestat] mean and std.dev: (1781.88, 2351.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12066) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (913, 1220, 1731) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3367) [M::mem_pestat] mean and std.dev: (1165.50, 599.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4185) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 937.061 CPU sec, 41.614 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666673, 48, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (387, 1533, 3504) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9738) [M::mem_pestat] mean and std.dev: (1807.00, 1713.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12855) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.16, 90.11) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 140, 1825) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5417) [M::mem_pestat] mean and std.dev: (731.63, 1338.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7213) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (261, 880, 1416) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3726) [M::mem_pestat] mean and std.dev: (832.89, 613.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4881) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 949.019 CPU sec, 55.546 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 667199, 47, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (418, 917, 1771) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4477) [M::mem_pestat] mean and std.dev: (1069.17, 810.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5830) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (370, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (128, 733) [M::mem_pestat] mean and std.dev: (432.01, 90.34) [M::mem_pestat] low and high boundaries for proper pairs: (7, 854) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 135, 1116) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3286) [M::mem_pestat] mean and std.dev: (493.76, 773.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4371) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1066, 1149, 3379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8005) [M::mem_pestat] mean and std.dev: (1972.33, 1692.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10318) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 946.160 CPU sec, 42.284 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 667277, 44, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (400, 697, 1709) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4327) [M::mem_pestat] mean and std.dev: (858.44, 663.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5636) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (370, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (128, 733) [M::mem_pestat] mean and std.dev: (432.23, 90.30) [M::mem_pestat] low and high boundaries for proper pairs: (7, 854) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 93, 1382) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4086) [M::mem_pestat] mean and std.dev: (399.24, 704.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5438) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (434, 962, 2002) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5138) [M::mem_pestat] mean and std.dev: (1132.31, 828.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6706) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 946.370 CPU sec, 55.957 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 666707, 40, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (368, 814, 2280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6104) [M::mem_pestat] mean and std.dev: (1261.31, 1566.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8016) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.07, 89.93) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 39, 2336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6984) [M::mem_pestat] mean and std.dev: (902.11, 1479.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9308) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 946.829 CPU sec, 42.216 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 666491, 42, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (317, 1107, 1634) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4268) [M::mem_pestat] mean and std.dev: (963.50, 676.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5585) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.48, 90.07) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 297, 2863) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8533) [M::mem_pestat] mean and std.dev: (1405.44, 2090.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11368) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 950.747 CPU sec, 55.720 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 666427, 42, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (239, 494, 1535) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4127) [M::mem_pestat] mean and std.dev: (761.05, 663.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5423) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.05, 90.00) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 62, 1623) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4825) [M::mem_pestat] mean and std.dev: (643.82, 1046.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6426) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (819, 1318, 2510) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5892) [M::mem_pestat] mean and std.dev: (1572.73, 1272.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7583) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 944.621 CPU sec, 42.299 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 667096, 43, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (330, 520, 924) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2112) [M::mem_pestat] mean and std.dev: (667.80, 447.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2706) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.24, 89.90) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 118, 1678) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4982) [M::mem_pestat] mean and std.dev: (989.29, 1505.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7013) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 953.396 CPU sec, 55.501 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 667072, 40, 14) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.23, 90.05) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 81, 1214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3600) [M::mem_pestat] mean and std.dev: (495.25, 886.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4793) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (803, 1191, 1772) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3710) [M::mem_pestat] mean and std.dev: (1212.38, 673.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4679) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 944.244 CPU sec, 42.027 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 666401, 35, 10) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.22, 89.93) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 120, 3345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9991) [M::mem_pestat] mean and std.dev: (1764.31, 2307.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13314) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (805, 1148, 1355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2455) [M::mem_pestat] mean and std.dev: (1026.60, 495.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3010) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 946.592 CPU sec, 55.158 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666402, 53, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (427, 538, 1493) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3625) [M::mem_pestat] mean and std.dev: (864.40, 615.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4691) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.04, 89.85) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 58, 890) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2620) [M::mem_pestat] mean and std.dev: (209.42, 410.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3485) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (591, 1071, 1784) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4170) [M::mem_pestat] mean and std.dev: (1016.46, 578.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5363) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 944.947 CPU sec, 41.998 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 666823, 30, 12) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.31, 90.16) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 70, 1461) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4339) [M::mem_pestat] mean and std.dev: (473.81, 758.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5778) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (948, 1646, 2930) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6894) [M::mem_pestat] mean and std.dev: (1931.36, 1520.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8876) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 945.887 CPU sec, 54.417 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 666382, 49, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (263, 917, 1818) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4928) [M::mem_pestat] mean and std.dev: (950.62, 941.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6483) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.21, 90.05) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 64, 1953) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5811) [M::mem_pestat] mean and std.dev: (802.47, 1204.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7740) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (985, 1780, 7511) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 20563) [M::mem_pestat] mean and std.dev: (2984.58, 3155.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 27089) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 946.457 CPU sec, 42.202 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 666818, 36, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (432, 1228, 1451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3489) [M::mem_pestat] mean and std.dev: (1013.20, 596.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4508) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 426, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.00, 89.99) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 498, 2642) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7886) [M::mem_pestat] mean and std.dev: (1203.89, 1443.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10508) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1248, 1797, 4448) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10848) [M::mem_pestat] mean and std.dev: (2860.09, 2729.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14048) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 951.991 CPU sec, 55.622 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666787, 42, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (358, 721, 2072) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5500) [M::mem_pestat] mean and std.dev: (1157.80, 1415.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7214) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.34, 90.03) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (10, 35, 1225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3655) [M::mem_pestat] mean and std.dev: (293.46, 681.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4870) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (919, 1015, 1975) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4087) [M::mem_pestat] mean and std.dev: (940.67, 485.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5143) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 942.602 CPU sec, 41.801 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 667214, 34, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (186, 603, 1122) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2994) [M::mem_pestat] mean and std.dev: (715.50, 640.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3930) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.23, 90.05) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 90, 2628) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7834) [M::mem_pestat] mean and std.dev: (1313.74, 1876.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10437) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1110, 1318, 2003) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3789) [M::mem_pestat] mean and std.dev: (1493.78, 783.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4682) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 942.391 CPU sec, 53.476 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 666546, 38, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (351, 807, 1360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3378) [M::mem_pestat] mean and std.dev: (846.80, 789.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4387) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (370, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (130, 730) [M::mem_pestat] mean and std.dev: (432.15, 90.07) [M::mem_pestat] low and high boundaries for proper pairs: (10, 850) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 133, 2668) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7976) [M::mem_pestat] mean and std.dev: (909.71, 1428.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10630) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1103, 1298, 2064) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3986) [M::mem_pestat] mean and std.dev: (1430.54, 651.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4947) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 946.039 CPU sec, 42.212 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 666636, 26, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (399, 1149, 2295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6087) [M::mem_pestat] mean and std.dev: (1232.00, 874.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7983) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.07, 89.92) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 541, 2884) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8608) [M::mem_pestat] mean and std.dev: (1891.81, 2349.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11470) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (871, 1179, 2355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5323) [M::mem_pestat] mean and std.dev: (1421.45, 774.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6807) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 949.813 CPU sec, 55.111 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 666466, 39, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (293, 760, 1680) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4454) [M::mem_pestat] mean and std.dev: (1227.00, 1213.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6080) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.04, 89.95) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 71, 1867) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5563) [M::mem_pestat] mean and std.dev: (763.47, 1186.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7411) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (783, 924, 2122) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4800) [M::mem_pestat] mean and std.dev: (1500.20, 1246.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6488) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 943.655 CPU sec, 41.984 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 666860, 45, 10) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (370, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (130, 730) [M::mem_pestat] mean and std.dev: (432.00, 90.09) [M::mem_pestat] low and high boundaries for proper pairs: (10, 850) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 74, 3252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9714) [M::mem_pestat] mean and std.dev: (1511.36, 2016.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12945) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (521, 1147, 1896) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4646) [M::mem_pestat] mean and std.dev: (877.50, 582.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6021) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 950.575 CPU sec, 55.551 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 666896, 32, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (357, 698, 1163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2775) [M::mem_pestat] mean and std.dev: (663.17, 390.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3581) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.10, 89.85) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (11, 30, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 542) [M::mem_pestat] mean and std.dev: (57.74, 106.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 719) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 939.153 CPU sec, 41.855 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 666792, 34, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (370, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (130, 730) [M::mem_pestat] mean and std.dev: (432.07, 90.18) [M::mem_pestat] low and high boundaries for proper pairs: (10, 850) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 33, 116) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 308) [M::mem_pestat] mean and std.dev: (35.27, 29.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 404) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 948.846 CPU sec, 55.044 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 666700, 47, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (403, 954, 1926) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4972) [M::mem_pestat] mean and std.dev: (1182.19, 1162.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6495) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.31, 90.02) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 98, 4329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12923) [M::mem_pestat] mean and std.dev: (1956.81, 2894.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17220) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 945.848 CPU sec, 41.965 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 666510, 31, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (290, 847, 1309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3347) [M::mem_pestat] mean and std.dev: (906.47, 653.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4366) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.41, 89.99) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (8, 25, 2022) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6050) [M::mem_pestat] mean and std.dev: (1139.83, 1644.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8064) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 944.037 CPU sec, 55.272 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666332, 38, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (832, 1013, 1123) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (250, 1705) [M::mem_pestat] mean and std.dev: (874.40, 365.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2338) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.18, 90.00) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 44, 742) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2194) [M::mem_pestat] mean and std.dev: (86.62, 172.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2920) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 940.614 CPU sec, 41.964 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 666618, 37, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (477, 715, 966) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1944) [M::mem_pestat] mean and std.dev: (609.73, 294.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2433) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.11, 90.03) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 49, 1708) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5078) [M::mem_pestat] mean and std.dev: (818.89, 1398.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6763) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 947.397 CPU sec, 54.885 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 666551, 31, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (346, 915, 1317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3259) [M::mem_pestat] mean and std.dev: (729.22, 554.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4230) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.27, 89.87) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (11, 70, 1851) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5531) [M::mem_pestat] mean and std.dev: (966.97, 1409.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7371) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (984, 1749, 2327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5013) [M::mem_pestat] mean and std.dev: (1957.73, 1258.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6991) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 946.654 CPU sec, 55.579 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 667256, 39, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (482, 1171, 4022) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11102) [M::mem_pestat] mean and std.dev: (2051.70, 2015.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14642) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.00, 89.84) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 393, 2513) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7497) [M::mem_pestat] mean and std.dev: (1180.74, 1704.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9989) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 942.528 CPU sec, 41.854 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 666557, 29, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (297, 485, 1448) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3750) [M::mem_pestat] mean and std.dev: (855.59, 848.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4901) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (370, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (130, 730) [M::mem_pestat] mean and std.dev: (432.02, 90.13) [M::mem_pestat] low and high boundaries for proper pairs: (10, 850) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (94, 583, 2346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6850) [M::mem_pestat] mean and std.dev: (1147.52, 1345.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9102) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 945.971 CPU sec, 53.792 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 666740, 52, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (381, 581, 1605) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4053) [M::mem_pestat] mean and std.dev: (884.12, 668.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5277) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (370, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (130, 730) [M::mem_pestat] mean and std.dev: (432.04, 90.07) [M::mem_pestat] low and high boundaries for proper pairs: (10, 850) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 271, 2760) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8218) [M::mem_pestat] mean and std.dev: (1363.24, 1829.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10947) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 949.033 CPU sec, 42.232 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 666754, 47, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (237, 519, 966) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2424) [M::mem_pestat] mean and std.dev: (520.10, 360.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3153) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.03, 89.81) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 48, 1575) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4687) [M::mem_pestat] mean and std.dev: (673.18, 1149.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6243) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 952.953 CPU sec, 55.326 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 667058, 47, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (319, 1142, 2191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5935) [M::mem_pestat] mean and std.dev: (1406.08, 1482.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7807) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.09, 89.95) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 156, 3373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10079) [M::mem_pestat] mean and std.dev: (1814.72, 2510.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13432) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (327, 354, 1282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3192) [M::mem_pestat] mean and std.dev: (699.36, 518.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4147) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 947.301 CPU sec, 42.106 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 666338, 48, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (370, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (130, 730) [M::mem_pestat] mean and std.dev: (432.13, 90.22) [M::mem_pestat] low and high boundaries for proper pairs: (10, 850) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 65, 2453) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7311) [M::mem_pestat] mean and std.dev: (1076.09, 1572.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9740) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 946.791 CPU sec, 55.878 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 667057, 35, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (375, 1166, 1226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2928) [M::mem_pestat] mean and std.dev: (798.78, 494.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3779) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.13, 89.91) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 45, 1371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4045) [M::mem_pestat] mean and std.dev: (445.27, 876.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5382) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 943.968 CPU sec, 41.905 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 667122, 35, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (303, 938, 1832) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4890) [M::mem_pestat] mean and std.dev: (1106.31, 1127.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6419) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (370, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (130, 730) [M::mem_pestat] mean and std.dev: (432.06, 90.13) [M::mem_pestat] low and high boundaries for proper pairs: (10, 850) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 43, 866) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2560) [M::mem_pestat] mean and std.dev: (343.53, 608.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3407) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1150, 1306, 2488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5164) [M::mem_pestat] mean and std.dev: (1401.60, 967.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6502) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 942.796 CPU sec, 53.755 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666658, 50, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (330, 853, 1143) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2769) [M::mem_pestat] mean and std.dev: (728.27, 413.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3582) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.15, 89.94) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 313, 3043) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9071) [M::mem_pestat] mean and std.dev: (1488.61, 1996.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12085) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (738, 999, 2299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5421) [M::mem_pestat] mean and std.dev: (1438.44, 959.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6982) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 947.062 CPU sec, 42.143 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 666573, 25, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (329, 466, 1424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3614) [M::mem_pestat] mean and std.dev: (727.08, 668.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4709) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.26, 89.92) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 291, 1628) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4824) [M::mem_pestat] mean and std.dev: (861.12, 1349.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6422) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1100, 1705, 4384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10952) [M::mem_pestat] mean and std.dev: (2442.38, 1908.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14236) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 951.584 CPU sec, 55.708 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 666561, 39, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (457, 1226, 2508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6610) [M::mem_pestat] mean and std.dev: (1701.35, 1553.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8661) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.41, 89.93) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 40, 706) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2086) [M::mem_pestat] mean and std.dev: (376.50, 607.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2808) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 945.732 CPU sec, 42.244 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 667186, 42, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (331, 492, 1080) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2578) [M::mem_pestat] mean and std.dev: (638.75, 476.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3327) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (370, 426, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (130, 730) [M::mem_pestat] mean and std.dev: (431.97, 90.11) [M::mem_pestat] low and high boundaries for proper pairs: (10, 850) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (10, 45, 952) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2836) [M::mem_pestat] mean and std.dev: (213.54, 500.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3778) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 941.418 CPU sec, 53.837 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 666676, 44, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (788, 953, 1715) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3569) [M::mem_pestat] mean and std.dev: (1171.50, 584.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4496) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (370, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (130, 730) [M::mem_pestat] mean and std.dev: (432.12, 90.08) [M::mem_pestat] low and high boundaries for proper pairs: (10, 850) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 40, 1703) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5075) [M::mem_pestat] mean and std.dev: (725.69, 1303.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6761) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (876, 1092, 2208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4872) [M::mem_pestat] mean and std.dev: (1270.43, 850.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6204) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 949.941 CPU sec, 42.213 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 666334, 34, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (405, 674, 1697) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4281) [M::mem_pestat] mean and std.dev: (863.89, 637.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5573) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.22, 90.01) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 378, 3366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10050) [M::mem_pestat] mean and std.dev: (1882.03, 2592.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13392) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 947.322 CPU sec, 53.676 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 666319, 37, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (335, 588, 1304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3242) [M::mem_pestat] mean and std.dev: (784.73, 822.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4211) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.08, 89.97) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 41, 1211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3601) [M::mem_pestat] mean and std.dev: (372.48, 855.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4796) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 945.719 CPU sec, 42.149 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 666851, 35, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.19, 89.91) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 121, 1852) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5494) [M::mem_pestat] mean and std.dev: (962.15, 1403.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7315) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 943.734 CPU sec, 54.519 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666665, 36, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (497, 1011, 2204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5618) [M::mem_pestat] mean and std.dev: (1157.90, 918.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7325) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.07, 89.85) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 38, 1943) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5797) [M::mem_pestat] mean and std.dev: (1017.29, 1460.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7724) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 939.032 CPU sec, 41.663 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 667262, 41, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (397, 911, 1379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3343) [M::mem_pestat] mean and std.dev: (803.93, 496.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4325) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.16, 90.03) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 81, 2370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7068) [M::mem_pestat] mean and std.dev: (933.18, 1542.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9417) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 944.273 CPU sec, 55.889 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 666904, 46, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (278, 325, 869) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2051) [M::mem_pestat] mean and std.dev: (438.88, 325.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2642) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (370, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (130, 730) [M::mem_pestat] mean and std.dev: (432.00, 90.05) [M::mem_pestat] low and high boundaries for proper pairs: (10, 850) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 39, 892) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2634) [M::mem_pestat] mean and std.dev: (343.56, 640.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3505) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 943.981 CPU sec, 41.951 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 666656, 31, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (367, 882, 1550) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3916) [M::mem_pestat] mean and std.dev: (803.44, 584.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5099) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.19, 90.03) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 68, 2161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6443) [M::mem_pestat] mean and std.dev: (1113.94, 1709.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8584) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (984, 1410, 2089) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4299) [M::mem_pestat] mean and std.dev: (1582.83, 1047.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5774) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 949.293 CPU sec, 55.987 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 666793, 39, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (522, 1137, 1531) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3549) [M::mem_pestat] mean and std.dev: (1164.40, 854.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4581) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (370, 426, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (130, 730) [M::mem_pestat] mean and std.dev: (432.02, 90.06) [M::mem_pestat] low and high boundaries for proper pairs: (10, 850) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 94, 1646) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4902) [M::mem_pestat] mean and std.dev: (666.42, 1063.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6530) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (656, 1054, 1278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2522) [M::mem_pestat] mean and std.dev: (831.50, 422.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3144) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 941.749 CPU sec, 41.871 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 666388, 36, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (279, 701, 1497) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3933) [M::mem_pestat] mean and std.dev: (790.90, 588.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5151) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (370, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (130, 730) [M::mem_pestat] mean and std.dev: (431.96, 90.10) [M::mem_pestat] low and high boundaries for proper pairs: (10, 850) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 110, 2173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6463) [M::mem_pestat] mean and std.dev: (1086.69, 1532.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8608) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 951.753 CPU sec, 56.148 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 667202, 29, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.22, 90.13) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 31, 882) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2606) [M::mem_pestat] mean and std.dev: (266.50, 455.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3468) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 945.287 CPU sec, 42.108 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 667054, 43, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (300, 924, 1384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3552) [M::mem_pestat] mean and std.dev: (684.17, 501.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4636) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.03, 89.89) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 152, 2376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7054) [M::mem_pestat] mean and std.dev: (961.87, 1444.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9393) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 947.477 CPU sec, 54.920 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 666574, 31, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.37, 90.13) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 67, 1795) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5351) [M::mem_pestat] mean and std.dev: (865.62, 1481.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7129) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 940.033 CPU sec, 41.782 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 667198, 30, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (403, 810, 1404) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3406) [M::mem_pestat] mean and std.dev: (832.56, 562.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4407) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.23, 89.95) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (11, 92, 2868) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8582) [M::mem_pestat] mean and std.dev: (1750.24, 2861.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13197) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (724, 1603, 2011) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4585) [M::mem_pestat] mean and std.dev: (1297.80, 772.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5872) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 943.919 CPU sec, 54.167 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666277, 43, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (370, 376, 957) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2131) [M::mem_pestat] mean and std.dev: (415.56, 218.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2718) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.20, 89.97) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 187, 2584) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7706) [M::mem_pestat] mean and std.dev: (1167.33, 1589.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10267) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 949.143 CPU sec, 42.195 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 666319, 32, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (203, 319, 794) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1976) [M::mem_pestat] mean and std.dev: (494.90, 468.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2567) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.42, 90.13) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 31, 1024) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3042) [M::mem_pestat] mean and std.dev: (359.04, 738.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4051) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 952.548 CPU sec, 55.345 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 667215, 48, 10) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.29, 90.18) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 45, 777) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2285) [M::mem_pestat] mean and std.dev: (413.04, 665.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3076) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (726, 1033, 1998) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4542) [M::mem_pestat] mean and std.dev: (1213.00, 815.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5814) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 941.479 CPU sec, 41.882 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 667333, 45, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (492, 650, 3887) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10677) [M::mem_pestat] mean and std.dev: (2197.08, 2635.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14072) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.19, 90.01) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 46, 1778) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5294) [M::mem_pestat] mean and std.dev: (872.30, 1600.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7273) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 944.345 CPU sec, 53.992 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 666509, 28, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (193, 437, 1387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3775) [M::mem_pestat] mean and std.dev: (831.30, 764.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4969) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.21, 90.08) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 116, 1450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4290) [M::mem_pestat] mean and std.dev: (700.52, 1056.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5710) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (406, 845, 1167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2689) [M::mem_pestat] mean and std.dev: (744.00, 405.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3450) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 937.763 CPU sec, 41.583 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 666933, 39, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (292, 1181, 2433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6715) [M::mem_pestat] mean and std.dev: (1554.07, 1503.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8856) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (370, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (130, 730) [M::mem_pestat] mean and std.dev: (432.09, 90.12) [M::mem_pestat] low and high boundaries for proper pairs: (10, 850) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 108, 2773) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8267) [M::mem_pestat] mean and std.dev: (1557.05, 1987.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11014) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (527, 1026, 1905) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4661) [M::mem_pestat] mean and std.dev: (1079.33, 908.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6039) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 950.184 CPU sec, 55.448 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 667070, 41, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (424, 975, 2443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6481) [M::mem_pestat] mean and std.dev: (1119.53, 972.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8500) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.28, 90.20) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 149, 2603) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7753) [M::mem_pestat] mean and std.dev: (1276.87, 1823.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10328) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 943.035 CPU sec, 42.015 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 666616, 36, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (465, 1182, 1371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3183) [M::mem_pestat] mean and std.dev: (1014.00, 547.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4089) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.18, 89.96) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 45, 1623) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4819) [M::mem_pestat] mean and std.dev: (472.84, 1008.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6417) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 943.409 CPU sec, 54.027 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 667081, 43, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (313, 1005, 2479) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6811) [M::mem_pestat] mean and std.dev: (1045.78, 912.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8977) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (370, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (128, 733) [M::mem_pestat] mean and std.dev: (432.21, 90.23) [M::mem_pestat] low and high boundaries for proper pairs: (7, 854) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 44, 1384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4112) [M::mem_pestat] mean and std.dev: (384.08, 779.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5476) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 943.315 CPU sec, 41.964 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 667377, 28, 14) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.23, 89.99) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 120, 3356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10038) [M::mem_pestat] mean and std.dev: (1206.25, 1690.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13379) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1109, 1526, 2902) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6488) [M::mem_pestat] mean and std.dev: (1730.08, 1109.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8281) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 941.633 CPU sec, 53.614 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 666551, 39, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (355, 806, 1356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3358) [M::mem_pestat] mean and std.dev: (863.94, 476.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4359) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.17, 89.83) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 65, 1985) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5901) [M::mem_pestat] mean and std.dev: (940.36, 1400.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7859) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 950.991 CPU sec, 42.648 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 666868, 38, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (394, 1053, 1837) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4723) [M::mem_pestat] mean and std.dev: (1207.38, 1080.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6166) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (370, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (130, 730) [M::mem_pestat] mean and std.dev: (432.16, 90.26) [M::mem_pestat] low and high boundaries for proper pairs: (10, 850) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 43, 2344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6986) [M::mem_pestat] mean and std.dev: (963.49, 1501.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9307) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (844, 2661, 4617) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12163) [M::mem_pestat] mean and std.dev: (3295.10, 2921.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15936) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 950.640 CPU sec, 55.349 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 666397, 25, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (382, 951, 1637) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4147) [M::mem_pestat] mean and std.dev: (1019.08, 831.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5402) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (370, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (128, 733) [M::mem_pestat] mean and std.dev: (432.17, 90.26) [M::mem_pestat] low and high boundaries for proper pairs: (7, 854) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 50, 2721) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8119) [M::mem_pestat] mean and std.dev: (1412.40, 1961.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10818) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (949, 1596, 2382) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5248) [M::mem_pestat] mean and std.dev: (1473.70, 915.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6681) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 950.937 CPU sec, 42.599 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 667674, 42, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (409, 894, 1742) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4408) [M::mem_pestat] mean and std.dev: (864.83, 600.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5741) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (370, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (128, 733) [M::mem_pestat] mean and std.dev: (432.30, 90.30) [M::mem_pestat] low and high boundaries for proper pairs: (7, 854) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 55, 1814) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5392) [M::mem_pestat] mean and std.dev: (803.31, 1392.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7181) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (547, 984, 2433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6205) [M::mem_pestat] mean and std.dev: (1666.00, 1695.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8448) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 946.386 CPU sec, 55.029 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 666849, 40, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (370, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (130, 730) [M::mem_pestat] mean and std.dev: (431.94, 89.93) [M::mem_pestat] low and high boundaries for proper pairs: (10, 850) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 91, 4123) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12315) [M::mem_pestat] mean and std.dev: (1759.67, 2372.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16411) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 941.871 CPU sec, 42.037 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 667537, 31, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (330, 658, 1473) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3759) [M::mem_pestat] mean and std.dev: (820.71, 645.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4902) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.18, 89.94) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 72, 1512) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4482) [M::mem_pestat] mean and std.dev: (691.66, 1121.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5967) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 940.497 CPU sec, 54.108 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 666565, 42, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (402, 1742, 2401) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6399) [M::mem_pestat] mean and std.dev: (1733.60, 1370.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8398) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.05, 89.91) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 106, 2738) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8170) [M::mem_pestat] mean and std.dev: (1559.98, 2207.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10886) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 943.105 CPU sec, 41.899 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 666463, 39, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (435, 1122, 2112) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5466) [M::mem_pestat] mean and std.dev: (1148.12, 791.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7143) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.04, 89.81) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 38, 1090) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3216) [M::mem_pestat] mean and std.dev: (363.50, 738.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4279) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (598, 933, 1409) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3031) [M::mem_pestat] mean and std.dev: (1064.92, 654.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3842) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 937.245 CPU sec, 53.931 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 666873, 40, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (420, 591, 1154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2622) [M::mem_pestat] mean and std.dev: (688.18, 476.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3356) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.26, 90.21) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 146, 1817) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5401) [M::mem_pestat] mean and std.dev: (893.97, 1319.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7193) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (804, 2021, 2932) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7188) [M::mem_pestat] mean and std.dev: (2114.80, 1565.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9316) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 941.945 CPU sec, 42.025 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 667161, 40, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (356, 703, 1462) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3674) [M::mem_pestat] mean and std.dev: (906.71, 654.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4780) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.16, 89.99) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (9, 37, 1610) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4812) [M::mem_pestat] mean and std.dev: (563.26, 1150.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6413) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (480, 1018, 2989) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8007) [M::mem_pestat] mean and std.dev: (1946.38, 1874.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10516) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 947.066 CPU sec, 55.332 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 667245, 32, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.08, 89.87) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 111, 4328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12952) [M::mem_pestat] mean and std.dev: (1483.94, 2514.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17264) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 944.237 CPU sec, 54.872 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666876, 33, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (449, 761, 1138) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2516) [M::mem_pestat] mean and std.dev: (721.20, 380.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3205) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (370, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (130, 730) [M::mem_pestat] mean and std.dev: (432.01, 90.04) [M::mem_pestat] low and high boundaries for proper pairs: (10, 850) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 45, 2210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6586) [M::mem_pestat] mean and std.dev: (712.90, 1278.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8774) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (699, 1087, 1287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2463) [M::mem_pestat] mean and std.dev: (1004.70, 450.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3051) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 942.675 CPU sec, 41.922 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666702, 57, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (433, 984, 2387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6295) [M::mem_pestat] mean and std.dev: (1328.45, 1067.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8249) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.13, 90.05) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 51, 2391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7133) [M::mem_pestat] mean and std.dev: (1022.82, 1556.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9504) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (599, 1344, 2252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5558) [M::mem_pestat] mean and std.dev: (1390.08, 1114.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7211) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 943.469 CPU sec, 54.410 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 667270, 46, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (230, 437, 4361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12623) [M::mem_pestat] mean and std.dev: (1963.50, 2653.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16754) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.27, 90.30) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 106, 1696) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5032) [M::mem_pestat] mean and std.dev: (796.77, 1332.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6700) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 945.693 CPU sec, 42.057 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 667034, 50, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (349, 581, 1568) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4006) [M::mem_pestat] mean and std.dev: (797.89, 598.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5225) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.14, 89.87) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 46, 1628) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4848) [M::mem_pestat] mean and std.dev: (774.52, 1249.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6458) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (636, 3237, 4037) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10839) [M::mem_pestat] mean and std.dev: (2599.50, 1974.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14240) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 944.993 CPU sec, 55.532 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 666539, 40, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.38, 90.16) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 334, 2308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6870) [M::mem_pestat] mean and std.dev: (1273.65, 1653.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9151) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 942.654 CPU sec, 42.090 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 666410, 36, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (560, 1385, 2393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6059) [M::mem_pestat] mean and std.dev: (1473.58, 1205.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7892) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.09, 89.97) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (56, 2390, 4363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12977) [M::mem_pestat] mean and std.dev: (2253.22, 2195.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17284) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 945.712 CPU sec, 55.656 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 666715, 37, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (290, 639, 4560) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13100) [M::mem_pestat] mean and std.dev: (2006.85, 2262.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17370) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.22, 89.95) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 82, 2754) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8226) [M::mem_pestat] mean and std.dev: (1231.46, 1550.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10962) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (433, 1413, 4011) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11167) [M::mem_pestat] mean and std.dev: (2503.14, 2771.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14745) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 946.529 CPU sec, 42.041 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 666672, 42, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (220, 456, 947) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2401) [M::mem_pestat] mean and std.dev: (584.67, 548.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3128) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.06, 90.05) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 58, 2818) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8414) [M::mem_pestat] mean and std.dev: (1109.07, 1788.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11212) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1343, 1957, 2500) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4814) [M::mem_pestat] mean and std.dev: (1815.54, 608.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5971) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 943.368 CPU sec, 54.115 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 666617, 47, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (337, 502, 1235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3031) [M::mem_pestat] mean and std.dev: (777.58, 741.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3929) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.20, 90.11) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 31, 955) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2835) [M::mem_pestat] mean and std.dev: (241.15, 535.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3775) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 942.242 CPU sec, 41.879 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 666815, 39, 12) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (370, 426, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (130, 730) [M::mem_pestat] mean and std.dev: (431.97, 90.04) [M::mem_pestat] low and high boundaries for proper pairs: (10, 850) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 49, 1790) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5334) [M::mem_pestat] mean and std.dev: (910.32, 1453.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7106) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (996, 1258, 3778) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9342) [M::mem_pestat] mean and std.dev: (1845.17, 1487.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12124) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 944.082 CPU sec, 54.067 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 666888, 43, 10) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.10, 89.94) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 249, 2916) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8706) [M::mem_pestat] mean and std.dev: (1425.40, 1664.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11601) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1209, 1389, 2204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4194) [M::mem_pestat] mean and std.dev: (1627.00, 821.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5189) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 943.755 CPU sec, 42.128 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 666922, 37, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (283, 1004, 1566) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4132) [M::mem_pestat] mean and std.dev: (914.77, 713.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5415) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.03, 89.87) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 84, 1779) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5285) [M::mem_pestat] mean and std.dev: (995.25, 1549.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7195) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1063, 1195, 2153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4333) [M::mem_pestat] mean and std.dev: (1535.20, 910.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5423) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 947.072 CPU sec, 54.500 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 666513, 43, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (639, 1632, 3517) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9273) [M::mem_pestat] mean and std.dev: (2077.38, 1670.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12151) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.18, 89.84) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 173, 2140) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6364) [M::mem_pestat] mean and std.dev: (1231.00, 1568.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8476) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 946.408 CPU sec, 42.243 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 666923, 44, 10) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.22, 90.02) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 62, 1098) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3262) [M::mem_pestat] mean and std.dev: (195.08, 405.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4344) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (349, 1247, 2480) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6742) [M::mem_pestat] mean and std.dev: (975.75, 818.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8873) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 948.810 CPU sec, 55.897 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 667378, 27, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (359, 527, 2851) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7835) [M::mem_pestat] mean and std.dev: (1439.42, 2006.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10327) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.26, 90.10) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 590, 2667) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7947) [M::mem_pestat] mean and std.dev: (1248.04, 1458.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10587) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 945.402 CPU sec, 41.991 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 666628, 43, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (370, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (130, 730) [M::mem_pestat] mean and std.dev: (432.13, 90.11) [M::mem_pestat] low and high boundaries for proper pairs: (10, 850) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 73, 938) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2762) [M::mem_pestat] mean and std.dev: (447.02, 728.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3674) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 953.740 CPU sec, 54.961 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 666323, 42, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (380, 474, 1709) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4367) [M::mem_pestat] mean and std.dev: (774.31, 643.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5696) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.29, 90.01) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 43, 1278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3806) [M::mem_pestat] mean and std.dev: (581.95, 1086.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5070) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1034, 1809, 2832) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6428) [M::mem_pestat] mean and std.dev: (2059.58, 1449.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8226) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 946.157 CPU sec, 42.219 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 666611, 40, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (309, 408, 1257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3153) [M::mem_pestat] mean and std.dev: (672.88, 462.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4101) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (370, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (130, 730) [M::mem_pestat] mean and std.dev: (432.02, 90.08) [M::mem_pestat] low and high boundaries for proper pairs: (10, 850) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 99, 2650) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7894) [M::mem_pestat] mean and std.dev: (980.24, 1520.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10516) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1396, 1780, 4216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9856) [M::mem_pestat] mean and std.dev: (2303.64, 1280.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12676) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 949.165 CPU sec, 54.657 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 667275, 41, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (906, 1315, 1793) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3567) [M::mem_pestat] mean and std.dev: (1266.60, 612.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4454) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (370, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (128, 733) [M::mem_pestat] mean and std.dev: (432.25, 90.31) [M::mem_pestat] low and high boundaries for proper pairs: (7, 854) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 74, 3114) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9270) [M::mem_pestat] mean and std.dev: (1355.46, 2256.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12348) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (484, 1022, 1855) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4597) [M::mem_pestat] mean and std.dev: (1294.27, 1090.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5968) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 948.847 CPU sec, 42.081 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 666781, 54, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (263, 566, 1206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3092) [M::mem_pestat] mean and std.dev: (616.15, 450.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4035) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.17, 90.05) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 36, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 983) [M::mem_pestat] mean and std.dev: (82.59, 163.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1305) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1163, 1487, 2278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4508) [M::mem_pestat] mean and std.dev: (1576.69, 1006.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5623) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 947.224 CPU sec, 53.479 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 666626, 35, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.52, 90.05) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 36, 1407) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4189) [M::mem_pestat] mean and std.dev: (617.36, 1091.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5580) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 948.256 CPU sec, 42.226 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 667295, 52, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (275, 326, 1258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3224) [M::mem_pestat] mean and std.dev: (693.70, 675.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4207) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.25, 89.95) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 336, 1835) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5459) [M::mem_pestat] mean and std.dev: (945.22, 1304.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7271) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (442, 1164, 2205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5731) [M::mem_pestat] mean and std.dev: (1279.00, 894.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7494) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 945.699 CPU sec, 54.139 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 666716, 44, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (329, 476, 1241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3065) [M::mem_pestat] mean and std.dev: (791.12, 661.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3977) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.11, 89.87) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 40, 1899) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5667) [M::mem_pestat] mean and std.dev: (895.61, 1432.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7551) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (491, 1142, 1741) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4241) [M::mem_pestat] mean and std.dev: (1211.75, 876.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5491) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 942.777 CPU sec, 42.033 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 667129, 52, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 426, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (431.92, 89.92) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 43, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 778) [M::mem_pestat] mean and std.dev: (69.45, 109.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1033) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 948.100 CPU sec, 53.053 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 667101, 42, 12) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.26, 90.28) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 630, 2618) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7792) [M::mem_pestat] mean and std.dev: (1475.83, 1797.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10379) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1078, 2367, 4878) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12478) [M::mem_pestat] mean and std.dev: (3016.17, 2651.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16278) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 943.431 CPU sec, 42.063 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 666822, 34, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (543, 858, 1505) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3429) [M::mem_pestat] mean and std.dev: (970.77, 774.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4391) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.25, 89.96) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 36, 615) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1815) [M::mem_pestat] mean and std.dev: (196.03, 388.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2415) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 950.912 CPU sec, 54.784 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666733, 34, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (401, 480, 1131) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2591) [M::mem_pestat] mean and std.dev: (632.36, 393.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3321) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (370, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (130, 730) [M::mem_pestat] mean and std.dev: (432.11, 90.25) [M::mem_pestat] low and high boundaries for proper pairs: (10, 850) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 122, 1755) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5225) [M::mem_pestat] mean and std.dev: (818.79, 1006.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6960) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (534, 982, 1706) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4050) [M::mem_pestat] mean and std.dev: (1071.85, 704.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5222) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 949.691 CPU sec, 42.353 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 666885, 42, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (146, 549, 1244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3440) [M::mem_pestat] mean and std.dev: (601.36, 568.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4538) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.16, 89.92) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 67, 2612) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7790) [M::mem_pestat] mean and std.dev: (1244.34, 1888.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10379) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (335, 944, 1734) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4532) [M::mem_pestat] mean and std.dev: (1195.25, 1077.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5931) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 947.827 CPU sec, 54.300 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 666407, 43, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (274, 876, 1603) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4261) [M::mem_pestat] mean and std.dev: (763.92, 633.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5590) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (370, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (128, 733) [M::mem_pestat] mean and std.dev: (432.22, 90.34) [M::mem_pestat] low and high boundaries for proper pairs: (7, 854) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 48, 1980) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5904) [M::mem_pestat] mean and std.dev: (1056.67, 1593.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7866) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (740, 1264, 2382) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5666) [M::mem_pestat] mean and std.dev: (1378.80, 820.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7308) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 946.269 CPU sec, 42.060 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 666945, 35, 15) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (370, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (128, 733) [M::mem_pestat] mean and std.dev: (432.14, 90.24) [M::mem_pestat] low and high boundaries for proper pairs: (7, 854) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 125, 1937) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5755) [M::mem_pestat] mean and std.dev: (1002.69, 1335.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7664) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (564, 982, 1895) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4557) [M::mem_pestat] mean and std.dev: (1189.87, 937.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5888) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 937.678 CPU sec, 52.843 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 667253, 48, 10) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (370, 426, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (130, 730) [M::mem_pestat] mean and std.dev: (431.94, 90.10) [M::mem_pestat] low and high boundaries for proper pairs: (10, 850) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 73, 3456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10320) [M::mem_pestat] mean and std.dev: (1805.19, 2811.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13752) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (805, 934, 1048) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (319, 1534) [M::mem_pestat] mean and std.dev: (849.71, 179.12) [M::mem_pestat] low and high boundaries for proper pairs: (76, 1777) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 937.336 CPU sec, 41.621 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 666818, 38, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (427, 558, 1678) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4180) [M::mem_pestat] mean and std.dev: (1024.69, 1010.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5431) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.11, 89.95) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 188, 2271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6761) [M::mem_pestat] mean and std.dev: (1104.86, 1531.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9006) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1189, 1547, 3668) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8626) [M::mem_pestat] mean and std.dev: (2031.00, 1232.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11105) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 939.616 CPU sec, 52.444 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 666928, 46, 13) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.05, 89.88) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 1221, 2919) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8689) [M::mem_pestat] mean and std.dev: (1618.98, 1811.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11574) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1033, 1163, 2996) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6922) [M::mem_pestat] mean and std.dev: (2111.00, 1769.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9188) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 932.892 CPU sec, 41.513 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 666624, 27, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (332, 979, 2317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6287) [M::mem_pestat] mean and std.dev: (1085.33, 1119.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8272) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.14, 89.89) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 67, 2284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6824) [M::mem_pestat] mean and std.dev: (1091.33, 1635.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9094) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (844, 1977, 2305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5227) [M::mem_pestat] mean and std.dev: (1507.33, 791.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6688) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 949.632 CPU sec, 54.957 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 666816, 42, 16) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.15, 90.06) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 273, 2435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7261) [M::mem_pestat] mean and std.dev: (1154.15, 1598.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9674) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (597, 1444, 2708) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6930) [M::mem_pestat] mean and std.dev: (1574.25, 1324.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9041) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 944.980 CPU sec, 42.032 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 666589, 38, 13) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.07, 89.89) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (11, 210, 1362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4064) [M::mem_pestat] mean and std.dev: (580.31, 956.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5415) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (428, 1139, 1687) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4205) [M::mem_pestat] mean and std.dev: (1082.83, 760.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5464) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 949.871 CPU sec, 55.600 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666264, 43, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (371, 501, 826) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1736) [M::mem_pestat] mean and std.dev: (444.67, 199.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2191) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (370, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (128, 733) [M::mem_pestat] mean and std.dev: (432.13, 90.28) [M::mem_pestat] low and high boundaries for proper pairs: (7, 854) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 147, 2590) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7722) [M::mem_pestat] mean and std.dev: (1111.44, 1536.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10288) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (679, 1092, 1621) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3505) [M::mem_pestat] mean and std.dev: (1210.50, 871.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4697) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 947.155 CPU sec, 42.031 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 666284, 32, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (304, 911, 1145) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2827) [M::mem_pestat] mean and std.dev: (723.31, 605.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3668) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.13, 89.89) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 201, 2924) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8724) [M::mem_pestat] mean and std.dev: (1437.03, 1835.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11624) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (836, 1048, 1761) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3611) [M::mem_pestat] mean and std.dev: (1160.50, 602.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4536) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 947.595 CPU sec, 55.157 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 667514, 38, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (381, 605, 1281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3081) [M::mem_pestat] mean and std.dev: (838.56, 704.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3981) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.30, 90.02) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 56, 1557) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4635) [M::mem_pestat] mean and std.dev: (684.44, 1234.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6174) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 940.782 CPU sec, 41.818 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 667008, 36, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.42, 90.14) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 274, 2283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6795) [M::mem_pestat] mean and std.dev: (1071.21, 1460.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9051) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 951.654 CPU sec, 55.699 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666270, 31, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (137, 258, 1309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3653) [M::mem_pestat] mean and std.dev: (504.90, 543.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4825) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.09, 89.84) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 152, 1537) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4557) [M::mem_pestat] mean and std.dev: (903.00, 1328.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6216) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (649, 1275, 1773) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4021) [M::mem_pestat] mean and std.dev: (1254.25, 851.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5145) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 948.776 CPU sec, 42.341 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 666518, 53, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (374, 551, 1166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2750) [M::mem_pestat] mean and std.dev: (684.00, 499.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3542) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.36, 90.17) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 57, 1798) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5340) [M::mem_pestat] mean and std.dev: (821.10, 1323.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7111) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (297, 458, 2740) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7626) [M::mem_pestat] mean and std.dev: (1251.27, 1127.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10069) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 946.486 CPU sec, 53.587 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 666658, 40, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (316, 972, 1950) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5218) [M::mem_pestat] mean and std.dev: (986.43, 839.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6852) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.13, 89.94) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 50, 1486) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4412) [M::mem_pestat] mean and std.dev: (770.39, 1343.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6143) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 941.282 CPU sec, 41.705 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666465, 37, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (517, 746, 2283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5815) [M::mem_pestat] mean and std.dev: (1227.82, 1009.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7581) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.29, 90.09) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 38, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951) [M::mem_pestat] mean and std.dev: (94.03, 159.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1263) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 943.337 CPU sec, 55.479 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 666850, 35, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (288, 408, 1949) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5271) [M::mem_pestat] mean and std.dev: (902.27, 815.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6932) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.27, 90.33) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 47, 1009) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2997) [M::mem_pestat] mean and std.dev: (386.39, 675.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3991) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 946.734 CPU sec, 42.154 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 666802, 47, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (395, 1295, 2584) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6962) [M::mem_pestat] mean and std.dev: (1422.00, 1131.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9151) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.28, 90.21) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 113, 2858) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8534) [M::mem_pestat] mean and std.dev: (1315.78, 1999.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11372) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 951.865 CPU sec, 56.431 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 667200, 32, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (404, 494, 1019) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2249) [M::mem_pestat] mean and std.dev: (642.00, 491.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2864) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.21, 90.04) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 697, 4364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13040) [M::mem_pestat] mean and std.dev: (2108.88, 2678.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17378) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (928, 1425, 1785) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3499) [M::mem_pestat] mean and std.dev: (1235.40, 762.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4356) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 942.280 CPU sec, 41.930 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 666719, 37, 12) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.28, 90.12) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 52, 1408) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4186) [M::mem_pestat] mean and std.dev: (595.37, 989.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5575) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1020, 1538, 2105) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4275) [M::mem_pestat] mean and std.dev: (1476.27, 968.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5360) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 946.796 CPU sec, 54.381 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 666717, 36, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (430, 1087, 3022) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8206) [M::mem_pestat] mean and std.dev: (1466.12, 1330.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10798) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.27, 90.10) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 34, 1600) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4774) [M::mem_pestat] mean and std.dev: (659.44, 1313.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6361) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (519, 1624, 2105) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5277) [M::mem_pestat] mean and std.dev: (1300.50, 823.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6863) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 946.052 CPU sec, 42.074 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 666697, 43, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (325, 544, 1060) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2530) [M::mem_pestat] mean and std.dev: (742.85, 656.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3370) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.27, 90.06) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 51, 3186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9506) [M::mem_pestat] mean and std.dev: (1857.07, 2830.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13180) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (721, 1052, 1566) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3256) [M::mem_pestat] mean and std.dev: (937.10, 352.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4101) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 950.715 CPU sec, 55.718 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 666984, 40, 11) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (431.99, 89.86) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 51, 1260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3736) [M::mem_pestat] mean and std.dev: (362.83, 631.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4974) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1106, 1414, 2583) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5537) [M::mem_pestat] mean and std.dev: (1590.27, 811.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7014) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 941.939 CPU sec, 41.947 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 667211, 36, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (332, 463, 1737) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4547) [M::mem_pestat] mean and std.dev: (815.15, 728.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5952) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.10, 89.96) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 71, 3497) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10435) [M::mem_pestat] mean and std.dev: (1568.31, 2543.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13904) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (381, 1389, 2361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6321) [M::mem_pestat] mean and std.dev: (1522.90, 1568.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8301) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 948.409 CPU sec, 54.226 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 666997, 36, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (464, 1166, 1512) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3608) [M::mem_pestat] mean and std.dev: (1009.00, 629.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4656) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.26, 90.03) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 64, 1772) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5276) [M::mem_pestat] mean and std.dev: (703.83, 1189.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7028) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (523, 974, 2520) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6514) [M::mem_pestat] mean and std.dev: (1526.69, 1511.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8511) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 946.831 CPU sec, 42.091 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 666662, 53, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (257, 455, 1088) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2750) [M::mem_pestat] mean and std.dev: (688.07, 685.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3581) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.20, 89.85) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 107, 2744) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8194) [M::mem_pestat] mean and std.dev: (1133.83, 1670.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10919) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (983, 1291, 2791) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6407) [M::mem_pestat] mean and std.dev: (1662.38, 1120.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8215) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 947.400 CPU sec, 55.747 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 667060, 33, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (485, 707, 1180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2570) [M::mem_pestat] mean and std.dev: (771.89, 384.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3265) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.30, 89.98) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 56, 1857) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5525) [M::mem_pestat] mean and std.dev: (993.68, 1573.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7359) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 946.726 CPU sec, 42.193 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 666746, 40, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (381, 955, 1945) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5073) [M::mem_pestat] mean and std.dev: (1501.25, 1612.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7950) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.06, 89.95) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 32, 2586) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7724) [M::mem_pestat] mean and std.dev: (907.74, 1537.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10293) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 944.052 CPU sec, 56.361 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 666699, 46, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (285, 578, 782) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1776) [M::mem_pestat] mean and std.dev: (559.33, 444.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2337) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.11, 89.94) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 47, 1453) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4335) [M::mem_pestat] mean and std.dev: (628.09, 1081.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5776) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (773, 1369, 2131) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4847) [M::mem_pestat] mean and std.dev: (1153.33, 697.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6205) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 948.232 CPU sec, 42.248 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 667263, 36, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.21, 89.90) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 40, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1295) [M::mem_pestat] mean and std.dev: (96.31, 237.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1721) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 944.101 CPU sec, 55.053 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 666426, 41, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (247, 371, 2430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6796) [M::mem_pestat] mean and std.dev: (1058.81, 1374.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8979) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.09, 90.07) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 48, 1432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4268) [M::mem_pestat] mean and std.dev: (548.87, 937.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5686) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 946.180 CPU sec, 53.176 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 667065, 42, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (322, 516, 1235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3061) [M::mem_pestat] mean and std.dev: (520.92, 344.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3974) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.14, 90.00) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 39, 2399) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7153) [M::mem_pestat] mean and std.dev: (956.50, 1663.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9530) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (629, 1499, 2203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5351) [M::mem_pestat] mean and std.dev: (1542.90, 1203.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6925) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 933.003 CPU sec, 41.472 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 667300, 37, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (611, 974, 1372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2894) [M::mem_pestat] mean and std.dev: (797.92, 413.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3655) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.10, 89.95) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 62, 2894) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8630) [M::mem_pestat] mean and std.dev: (1312.49, 2005.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11498) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (964, 1133, 1742) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3298) [M::mem_pestat] mean and std.dev: (1282.73, 740.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4243) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 936.996 CPU sec, 54.339 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 666553, 42, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (237, 451, 752) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1782) [M::mem_pestat] mean and std.dev: (598.92, 483.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2532) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.06, 90.00) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 45, 824) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2428) [M::mem_pestat] mean and std.dev: (304.75, 537.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3230) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (336, 965, 2937) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8139) [M::mem_pestat] mean and std.dev: (1985.40, 2157.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10740) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 938.718 CPU sec, 41.715 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 667150, 45, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (402, 905, 1948) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5040) [M::mem_pestat] mean and std.dev: (1085.60, 935.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6586) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.15, 90.08) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 46, 1537) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4577) [M::mem_pestat] mean and std.dev: (675.67, 1308.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6097) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (834, 1140, 3826) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9810) [M::mem_pestat] mean and std.dev: (1882.81, 1588.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12802) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 944.341 CPU sec, 55.485 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 666961, 34, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (405, 1034, 2262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5976) [M::mem_pestat] mean and std.dev: (1374.60, 1362.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7833) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (431.89, 89.91) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 103, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689) [M::mem_pestat] mean and std.dev: (86.44, 115.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 914) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (790, 1435, 2125) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4795) [M::mem_pestat] mean and std.dev: (1547.85, 1150.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6150) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 947.179 CPU sec, 42.265 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666739, 46, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (722, 1373, 3454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8918) [M::mem_pestat] mean and std.dev: (2357.55, 2483.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12293) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.02, 89.80) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 225, 2627) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7821) [M::mem_pestat] mean and std.dev: (1061.74, 1550.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10418) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (502, 1114, 1319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2953) [M::mem_pestat] mean and std.dev: (947.92, 580.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3770) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 948.396 CPU sec, 54.263 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 666768, 50, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (343, 1014, 1511) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3847) [M::mem_pestat] mean and std.dev: (1045.70, 808.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5015) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.05, 89.96) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 60, 2912) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8704) [M::mem_pestat] mean and std.dev: (1397.08, 2054.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11600) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 947.479 CPU sec, 42.160 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 666916, 39, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (405, 722, 1665) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4185) [M::mem_pestat] mean and std.dev: (1011.23, 773.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5445) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.05, 89.96) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 156, 2424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7210) [M::mem_pestat] mean and std.dev: (1068.18, 1401.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9603) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (922, 2548, 4604) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11968) [M::mem_pestat] mean and std.dev: (2805.40, 2224.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15650) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 949.585 CPU sec, 54.978 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 667032, 44, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (513, 1015, 2472) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6390) [M::mem_pestat] mean and std.dev: (1532.67, 1389.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8349) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.06, 90.01) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 67, 1208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3578) [M::mem_pestat] mean and std.dev: (493.20, 787.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4763) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (829, 1263, 1651) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3295) [M::mem_pestat] mean and std.dev: (1079.89, 498.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4117) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 940.594 CPU sec, 41.925 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 666807, 38, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (609, 998, 1712) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3918) [M::mem_pestat] mean and std.dev: (974.67, 553.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5021) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.20, 89.95) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 116, 3271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9773) [M::mem_pestat] mean and std.dev: (1932.24, 2765.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13024) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (490, 1715, 1847) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4561) [M::mem_pestat] mean and std.dev: (1334.44, 832.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5918) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 941.338 CPU sec, 53.418 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 667246, 37, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (413, 539, 978) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2108) [M::mem_pestat] mean and std.dev: (614.92, 375.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2673) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.14, 90.06) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 169, 3188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9518) [M::mem_pestat] mean and std.dev: (1549.78, 2256.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12683) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 939.007 CPU sec, 41.583 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 666810, 43, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (324, 503, 1217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3003) [M::mem_pestat] mean and std.dev: (820.07, 741.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3896) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.20, 89.88) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 119, 1921) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5737) [M::mem_pestat] mean and std.dev: (960.88, 1656.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7645) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 948.135 CPU sec, 53.314 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666847, 42, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (282, 328, 1108) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2760) [M::mem_pestat] mean and std.dev: (691.00, 563.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3586) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.11, 89.98) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 60, 2441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7269) [M::mem_pestat] mean and std.dev: (978.73, 1596.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9683) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 940.152 CPU sec, 41.730 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 667300, 32, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (302, 681, 2457) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6767) [M::mem_pestat] mean and std.dev: (1168.85, 1126.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8922) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (370, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (130, 730) [M::mem_pestat] mean and std.dev: (432.13, 90.18) [M::mem_pestat] low and high boundaries for proper pairs: (10, 850) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 138, 4367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13077) [M::mem_pestat] mean and std.dev: (1848.41, 2642.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17432) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1032, 1661, 2811) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6369) [M::mem_pestat] mean and std.dev: (1780.36, 895.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8148) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 945.492 CPU sec, 54.738 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 667005, 39, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.22, 90.06) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 230, 2637) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7885) [M::mem_pestat] mean and std.dev: (1258.95, 1607.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10509) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 944.255 CPU sec, 42.089 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 666717, 44, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (354, 1576, 2853) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7851) [M::mem_pestat] mean and std.dev: (2284.60, 2513.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12339) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 426, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (431.99, 89.92) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 73, 4217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12607) [M::mem_pestat] mean and std.dev: (1651.45, 2359.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16802) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (927, 1582, 6614) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 17988) [M::mem_pestat] mean and std.dev: (3061.54, 2945.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 23675) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 948.024 CPU sec, 55.443 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 666692, 46, 12) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.38, 90.17) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 76, 2609) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7785) [M::mem_pestat] mean and std.dev: (1091.78, 1497.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10373) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (921, 1046, 1353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (57, 2217) [M::mem_pestat] mean and std.dev: (997.30, 310.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2649) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 940.253 CPU sec, 41.646 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 666866, 53, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (820, 1228, 2445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5695) [M::mem_pestat] mean and std.dev: (1303.71, 945.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7320) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.10, 89.94) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 42, 1105) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3277) [M::mem_pestat] mean and std.dev: (290.40, 621.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4363) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (732, 1158, 1844) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4068) [M::mem_pestat] mean and std.dev: (951.80, 486.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5180) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 944.769 CPU sec, 53.866 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 666835, 39, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (351, 664, 2858) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7872) [M::mem_pestat] mean and std.dev: (985.45, 939.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10379) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.22, 89.81) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (52, 834, 4356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12964) [M::mem_pestat] mean and std.dev: (2230.62, 2808.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17268) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (448, 1230, 1705) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4219) [M::mem_pestat] mean and std.dev: (1229.60, 938.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5476) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 949.407 CPU sec, 42.257 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 666635, 31, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (351, 505, 1068) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2502) [M::mem_pestat] mean and std.dev: (695.80, 502.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3219) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.11, 89.89) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 34, 1499) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4457) [M::mem_pestat] mean and std.dev: (540.32, 1167.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5936) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 946.186 CPU sec, 53.934 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 666577, 41, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (255, 684, 2524) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7062) [M::mem_pestat] mean and std.dev: (1609.21, 1779.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9331) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.09, 89.88) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 116, 1542) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4572) [M::mem_pestat] mean and std.dev: (850.05, 1305.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6087) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 943.093 CPU sec, 41.864 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 666583, 34, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (361, 828, 1946) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5116) [M::mem_pestat] mean and std.dev: (1227.67, 1300.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6701) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.12, 89.92) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 122, 2213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6607) [M::mem_pestat] mean and std.dev: (1180.18, 1575.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8804) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (858, 1073, 1529) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2871) [M::mem_pestat] mean and std.dev: (1183.60, 510.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3542) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 944.581 CPU sec, 54.588 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 667110, 38, 18) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.03, 89.94) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 53, 2402) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7176) [M::mem_pestat] mean and std.dev: (1003.41, 1491.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9563) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (517, 1184, 1975) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4891) [M::mem_pestat] mean and std.dev: (1512.89, 1169.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6349) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 937.305 CPU sec, 41.721 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 666641, 42, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (359, 490, 2123) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5651) [M::mem_pestat] mean and std.dev: (1210.31, 1260.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7415) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.29, 89.85) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 443, 3450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10294) [M::mem_pestat] mean and std.dev: (1752.50, 2218.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13716) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (858, 1310, 3159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7761) [M::mem_pestat] mean and std.dev: (1518.80, 1103.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10062) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 942.848 CPU sec, 54.512 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 667066, 35, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (364, 397, 1499) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3769) [M::mem_pestat] mean and std.dev: (791.69, 547.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4904) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.32, 89.90) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 43, 788) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2324) [M::mem_pestat] mean and std.dev: (241.10, 525.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3092) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (706, 1263, 2857) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7159) [M::mem_pestat] mean and std.dev: (1733.21, 1339.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9310) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 940.575 CPU sec, 41.871 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 666417, 36, 10) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.09, 89.90) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 48, 2641) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7867) [M::mem_pestat] mean and std.dev: (1203.09, 2012.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10480) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (817, 1333, 1538) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2980) [M::mem_pestat] mean and std.dev: (987.75, 425.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3701) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 944.992 CPU sec, 53.868 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 666496, 44, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (119, 356, 485) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1217) [M::mem_pestat] mean and std.dev: (361.22, 304.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1583) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (370, 426, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (130, 730) [M::mem_pestat] mean and std.dev: (431.82, 89.96) [M::mem_pestat] low and high boundaries for proper pairs: (10, 850) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 214, 2790) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8314) [M::mem_pestat] mean and std.dev: (1364.50, 1905.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11076) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 940.816 CPU sec, 41.769 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 666863, 38, 11) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.03, 89.93) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 1327, 3439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10261) [M::mem_pestat] mean and std.dev: (1643.92, 1790.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13672) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (802, 1202, 1482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2842) [M::mem_pestat] mean and std.dev: (1111.91, 609.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3550) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 944.378 CPU sec, 54.939 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 667457, 36, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (381, 952, 1589) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4005) [M::mem_pestat] mean and std.dev: (1009.00, 864.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5213) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.35, 90.05) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 61, 1632) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4858) [M::mem_pestat] mean and std.dev: (806.06, 1236.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6471) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (848, 1870, 2677) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6335) [M::mem_pestat] mean and std.dev: (1980.88, 1611.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8426) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 940.107 CPU sec, 41.912 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 666962, 37, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (480, 950, 1311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2973) [M::mem_pestat] mean and std.dev: (697.38, 312.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3804) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.35, 90.05) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 43, 721) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2137) [M::mem_pestat] mean and std.dev: (161.58, 304.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2845) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1868, 2382, 3245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5999) [M::mem_pestat] mean and std.dev: (2654.83, 1208.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7490) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 944.283 CPU sec, 55.400 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 666684, 37, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (393, 790, 2538) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6828) [M::mem_pestat] mean and std.dev: (1315.62, 1258.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8973) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.11, 90.03) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (11, 87, 3589) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10745) [M::mem_pestat] mean and std.dev: (1505.24, 1920.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14323) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 947.677 CPU sec, 42.293 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 667094, 41, 15) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.16, 90.04) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 94, 2622) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7838) [M::mem_pestat] mean and std.dev: (1051.78, 1429.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10446) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1015, 1299, 2053) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4129) [M::mem_pestat] mean and std.dev: (1436.71, 967.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5308) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 945.673 CPU sec, 53.945 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 666857, 38, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (393, 471, 1738) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4428) [M::mem_pestat] mean and std.dev: (1034.07, 879.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5773) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.06, 89.90) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (41, 1209, 3221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9581) [M::mem_pestat] mean and std.dev: (1632.79, 1743.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12761) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (809, 1406, 2549) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6029) [M::mem_pestat] mean and std.dev: (1636.58, 1440.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7769) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 942.967 CPU sec, 41.985 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 666862, 36, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (310, 449, 1206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2998) [M::mem_pestat] mean and std.dev: (753.95, 747.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3894) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (370, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (130, 730) [M::mem_pestat] mean and std.dev: (432.20, 89.99) [M::mem_pestat] low and high boundaries for proper pairs: (10, 850) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 167, 2577) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7681) [M::mem_pestat] mean and std.dev: (1250.15, 1784.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10233) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 942.898 CPU sec, 53.442 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 666829, 49, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (338, 609, 936) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2132) [M::mem_pestat] mean and std.dev: (531.09, 283.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2730) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.24, 90.02) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 59, 2077) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6173) [M::mem_pestat] mean and std.dev: (878.60, 1436.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8221) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1181, 1687, 2646) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5576) [M::mem_pestat] mean and std.dev: (1853.33, 1046.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7041) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 942.341 CPU sec, 41.941 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 666961, 31, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (326, 418, 1712) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4484) [M::mem_pestat] mean and std.dev: (1053.00, 1019.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5870) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (431.98, 89.87) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 49, 2268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6760) [M::mem_pestat] mean and std.dev: (916.40, 1385.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9006) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 939.847 CPU sec, 53.294 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 666469, 34, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (386, 524, 1332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3224) [M::mem_pestat] mean and std.dev: (814.33, 644.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4170) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (370, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (130, 730) [M::mem_pestat] mean and std.dev: (431.96, 90.05) [M::mem_pestat] low and high boundaries for proper pairs: (10, 850) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 1167, 3558) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10606) [M::mem_pestat] mean and std.dev: (1746.21, 2197.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14130) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (724, 1313, 1506) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3070) [M::mem_pestat] mean and std.dev: (1108.40, 477.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3852) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 945.337 CPU sec, 41.964 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 665850, 39, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (261, 382, 1079) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2715) [M::mem_pestat] mean and std.dev: (802.40, 757.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3834) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.12, 89.90) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (45, 100, 1910) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5640) [M::mem_pestat] mean and std.dev: (719.64, 1082.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7505) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (920, 1142, 1980) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4100) [M::mem_pestat] mean and std.dev: (1198.06, 697.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5160) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 946.718 CPU sec, 55.079 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 666519, 34, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (289, 483, 1564) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4114) [M::mem_pestat] mean and std.dev: (676.13, 592.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5389) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.13, 89.99) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 230, 4351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12979) [M::mem_pestat] mean and std.dev: (2054.09, 2722.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17293) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 941.788 CPU sec, 41.864 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 667030, 47, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (345, 1030, 1472) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3726) [M::mem_pestat] mean and std.dev: (890.85, 651.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4853) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 426, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.02, 89.97) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 125, 2575) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7675) [M::mem_pestat] mean and std.dev: (968.42, 1408.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10225) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (695, 858, 1598) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3404) [M::mem_pestat] mean and std.dev: (1063.36, 588.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4307) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 941.354 CPU sec, 54.740 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 666888, 58, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (345, 520, 1192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2886) [M::mem_pestat] mean and std.dev: (664.82, 486.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3733) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (370, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (130, 730) [M::mem_pestat] mean and std.dev: (432.11, 90.14) [M::mem_pestat] low and high boundaries for proper pairs: (10, 850) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 47, 1427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4247) [M::mem_pestat] mean and std.dev: (253.40, 700.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5657) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1380, 1611, 7673) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 20259) [M::mem_pestat] mean and std.dev: (3196.27, 3002.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 26552) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 946.729 CPU sec, 42.125 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 666707, 43, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (369, 428, 1389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3429) [M::mem_pestat] mean and std.dev: (779.00, 589.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4449) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.34, 90.05) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 105, 1921) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5715) [M::mem_pestat] mean and std.dev: (1206.00, 1658.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7839) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (611, 1425, 1924) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4550) [M::mem_pestat] mean and std.dev: (1291.90, 824.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5863) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 941.084 CPU sec, 53.212 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 666782, 40, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (284, 1343, 1930) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5222) [M::mem_pestat] mean and std.dev: (1428.50, 1132.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6868) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.31, 90.20) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 506, 4387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13127) [M::mem_pestat] mean and std.dev: (2399.28, 3165.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17497) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 944.473 CPU sec, 42.003 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 666886, 37, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (401, 796, 1433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3497) [M::mem_pestat] mean and std.dev: (991.12, 914.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4648) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.11, 89.95) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 46, 1652) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4910) [M::mem_pestat] mean and std.dev: (653.29, 1160.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6539) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 944.865 CPU sec, 55.201 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666955, 41, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (379, 493, 1005) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2257) [M::mem_pestat] mean and std.dev: (517.78, 269.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2883) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.37, 90.06) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 50, 1667) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4967) [M::mem_pestat] mean and std.dev: (634.55, 1111.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6617) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 942.406 CPU sec, 41.996 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 666836, 34, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (223, 1163, 2371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6667) [M::mem_pestat] mean and std.dev: (1382.82, 1442.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8815) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.15, 90.02) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 194, 1645) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4877) [M::mem_pestat] mean and std.dev: (740.71, 1120.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6493) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 946.041 CPU sec, 55.965 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 667000, 45, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (385, 459, 1220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2890) [M::mem_pestat] mean and std.dev: (517.00, 278.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3725) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.07, 89.86) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 70, 1854) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5518) [M::mem_pestat] mean and std.dev: (962.70, 1376.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7350) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (979, 1154, 2423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5311) [M::mem_pestat] mean and std.dev: (1419.40, 796.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6755) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 943.788 CPU sec, 53.351 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 666970, 29, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (613, 1025, 1545) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3409) [M::mem_pestat] mean and std.dev: (1074.58, 715.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4341) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.14, 90.10) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 121, 4362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13024) [M::mem_pestat] mean and std.dev: (2132.00, 2664.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17355) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (395, 850, 2061) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5393) [M::mem_pestat] mean and std.dev: (1375.69, 1496.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7361) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 940.119 CPU sec, 41.897 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666636, 40, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (347, 956, 1135) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2711) [M::mem_pestat] mean and std.dev: (713.70, 419.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3499) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.01, 90.01) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 313, 2298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6826) [M::mem_pestat] mean and std.dev: (1240.00, 1774.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9090) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 943.562 CPU sec, 53.621 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 667019, 36, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (304, 895, 4972) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14308) [M::mem_pestat] mean and std.dev: (1885.25, 2308.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18976) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.20, 90.13) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 1294, 4342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12960) [M::mem_pestat] mean and std.dev: (2027.56, 2465.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17269) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 939.250 CPU sec, 41.736 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 666732, 37, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (385, 762, 1854) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4792) [M::mem_pestat] mean and std.dev: (1074.90, 968.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6261) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (370, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (130, 730) [M::mem_pestat] mean and std.dev: (432.15, 90.12) [M::mem_pestat] low and high boundaries for proper pairs: (10, 850) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 43, 1874) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5586) [M::mem_pestat] mean and std.dev: (839.14, 1555.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7442) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 944.976 CPU sec, 55.068 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 666790, 36, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (442, 862, 1880) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4756) [M::mem_pestat] mean and std.dev: (1201.53, 941.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6194) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (370, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (130, 730) [M::mem_pestat] mean and std.dev: (432.14, 90.16) [M::mem_pestat] low and high boundaries for proper pairs: (10, 850) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 139, 1777) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5287) [M::mem_pestat] mean and std.dev: (1064.78, 1503.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7079) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (569, 858, 2708) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6986) [M::mem_pestat] mean and std.dev: (1648.27, 1481.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9125) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 943.026 CPU sec, 42.123 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 666533, 53, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (220, 463, 1355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3625) [M::mem_pestat] mean and std.dev: (672.50, 726.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4760) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.26, 90.02) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 231, 2365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7047) [M::mem_pestat] mean and std.dev: (1169.60, 1517.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9388) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 943.239 CPU sec, 54.186 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 667434, 47, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (415, 863, 1426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3448) [M::mem_pestat] mean and std.dev: (916.78, 576.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4459) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.09, 89.81) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 68, 613) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1795) [M::mem_pestat] mean and std.dev: (148.68, 261.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2386) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (841, 1536, 2968) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7222) [M::mem_pestat] mean and std.dev: (1670.73, 1309.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9349) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 943.706 CPU sec, 42.111 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 666051, 41, 11) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.26, 89.99) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 93, 3068) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9162) [M::mem_pestat] mean and std.dev: (1533.76, 2104.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12209) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (926, 1053, 1293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (192, 2027) [M::mem_pestat] mean and std.dev: (1072.00, 150.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2394) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 950.439 CPU sec, 55.009 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 666728, 41, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (397, 825, 1457) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3577) [M::mem_pestat] mean and std.dev: (907.77, 680.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4637) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.18, 90.03) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 61, 3297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9835) [M::mem_pestat] mean and std.dev: (1664.29, 2554.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13104) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (986, 1280, 1956) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3896) [M::mem_pestat] mean and std.dev: (1347.00, 720.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4866) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 939.381 CPU sec, 41.886 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 665639, 39, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (293, 730, 1720) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4574) [M::mem_pestat] mean and std.dev: (859.53, 642.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6001) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (370, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (130, 730) [M::mem_pestat] mean and std.dev: (431.94, 89.97) [M::mem_pestat] low and high boundaries for proper pairs: (10, 850) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 49, 1494) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4420) [M::mem_pestat] mean and std.dev: (934.26, 1460.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6775) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (464, 805, 1109) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2399) [M::mem_pestat] mean and std.dev: (844.45, 515.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3044) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 949.115 CPU sec, 55.934 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 667027, 34, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (361, 1266, 2002) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5284) [M::mem_pestat] mean and std.dev: (1258.93, 864.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6925) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.08, 89.93) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 206, 2017) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5981) [M::mem_pestat] mean and std.dev: (1060.09, 1628.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7963) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1215, 1609, 2032) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3666) [M::mem_pestat] mean and std.dev: (1281.27, 585.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4483) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 948.618 CPU sec, 42.067 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 666700, 40, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (408, 520, 1641) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4107) [M::mem_pestat] mean and std.dev: (737.15, 625.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5340) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (370, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (130, 730) [M::mem_pestat] mean and std.dev: (432.13, 90.12) [M::mem_pestat] low and high boundaries for proper pairs: (10, 850) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 45, 1932) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5770) [M::mem_pestat] mean and std.dev: (990.05, 1515.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7689) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1013, 1488, 1709) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3101) [M::mem_pestat] mean and std.dev: (1321.14, 558.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3797) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 952.365 CPU sec, 55.277 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666872, 41, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (356, 636, 3880) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10928) [M::mem_pestat] mean and std.dev: (1923.00, 2083.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14452) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.17, 89.90) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 69, 1541) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4591) [M::mem_pestat] mean and std.dev: (501.66, 874.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6116) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (374, 1310, 2789) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7619) [M::mem_pestat] mean and std.dev: (1547.43, 1088.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10034) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 944.338 CPU sec, 42.067 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 666834, 36, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (536, 722, 1825) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4403) [M::mem_pestat] mean and std.dev: (1165.35, 1057.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5692) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.13, 89.88) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 45, 1802) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5366) [M::mem_pestat] mean and std.dev: (897.06, 1369.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7148) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 946.197 CPU sec, 53.795 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 666516, 49, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (357, 623, 1292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3162) [M::mem_pestat] mean and std.dev: (752.08, 452.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4097) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.44, 90.04) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 83, 1891) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5643) [M::mem_pestat] mean and std.dev: (758.43, 1260.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7519) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (768, 1163, 3743) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9693) [M::mem_pestat] mean and std.dev: (2010.35, 1669.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12668) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 940.489 CPU sec, 41.794 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 666389, 32, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (283, 462, 1039) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2551) [M::mem_pestat] mean and std.dev: (577.78, 516.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3307) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.12, 89.91) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 62, 1861) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5557) [M::mem_pestat] mean and std.dev: (990.94, 1587.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7405) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (908, 1486, 1946) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4022) [M::mem_pestat] mean and std.dev: (1318.73, 683.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5060) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 953.291 CPU sec, 55.619 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 666803, 44, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (417, 936, 1715) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4311) [M::mem_pestat] mean and std.dev: (1210.27, 1110.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5654) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.26, 89.98) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 117, 2219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6613) [M::mem_pestat] mean and std.dev: (1026.02, 1498.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8810) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 944.789 CPU sec, 42.041 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 666284, 42, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (376, 717, 1252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3004) [M::mem_pestat] mean and std.dev: (752.06, 426.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3880) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.30, 89.86) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 113, 1727) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5121) [M::mem_pestat] mean and std.dev: (1078.00, 1577.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7389) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 951.971 CPU sec, 56.058 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 666916, 37, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.21, 90.23) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 268, 2633) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7855) [M::mem_pestat] mean and std.dev: (1136.22, 1579.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10466) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 941.278 CPU sec, 41.785 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 667107, 27, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (383, 551, 1483) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3683) [M::mem_pestat] mean and std.dev: (818.00, 557.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4783) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.12, 89.99) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 131, 2629) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7849) [M::mem_pestat] mean and std.dev: (1461.41, 1933.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10459) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 945.206 CPU sec, 55.532 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 666940, 44, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (319, 682, 1241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3085) [M::mem_pestat] mean and std.dev: (874.67, 729.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4007) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.19, 89.94) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 152, 2887) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8615) [M::mem_pestat] mean and std.dev: (1457.38, 2384.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11479) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 946.139 CPU sec, 42.226 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 667011, 39, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (289, 627, 1851) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4975) [M::mem_pestat] mean and std.dev: (1060.52, 1192.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6537) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.10, 89.84) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 395, 4367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13037) [M::mem_pestat] mean and std.dev: (2182.72, 2798.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17372) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (889, 1245, 1872) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3838) [M::mem_pestat] mean and std.dev: (1273.82, 640.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4821) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 949.524 CPU sec, 56.036 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 666916, 44, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (399, 775, 1144) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2634) [M::mem_pestat] mean and std.dev: (721.75, 475.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3379) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 426, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (431.99, 89.94) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 111, 1779) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5287) [M::mem_pestat] mean and std.dev: (879.34, 1342.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7041) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 944.646 CPU sec, 42.063 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666843, 38, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (417, 955, 4176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11694) [M::mem_pestat] mean and std.dev: (2450.82, 3099.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15453) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.14, 90.08) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (8, 33, 2407) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7205) [M::mem_pestat] mean and std.dev: (1053.92, 1565.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9604) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (669, 1006, 1688) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3726) [M::mem_pestat] mean and std.dev: (1198.91, 821.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4745) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 952.850 CPU sec, 55.583 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 666667, 43, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (326, 499, 1542) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3974) [M::mem_pestat] mean and std.dev: (789.62, 706.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5190) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (370, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (130, 730) [M::mem_pestat] mean and std.dev: (431.99, 90.04) [M::mem_pestat] low and high boundaries for proper pairs: (10, 850) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 74, 3232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9650) [M::mem_pestat] mean and std.dev: (1615.70, 2380.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12859) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (503, 1285, 2471) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6407) [M::mem_pestat] mean and std.dev: (1366.55, 927.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8375) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 947.495 CPU sec, 42.275 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 666299, 36, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1090, 1570, 2533) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5419) [M::mem_pestat] mean and std.dev: (1830.42, 1388.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7386) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.17, 90.09) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 106, 2864) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8552) [M::mem_pestat] mean and std.dev: (1122.14, 1548.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11396) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 950.198 CPU sec, 55.355 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666408, 33, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (311, 403, 901) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2081) [M::mem_pestat] mean and std.dev: (593.20, 544.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2770) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.25, 90.36) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 89, 2945) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8783) [M::mem_pestat] mean and std.dev: (1178.88, 1656.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11702) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 947.778 CPU sec, 42.323 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 667222, 51, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (334, 1129, 2539) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6949) [M::mem_pestat] mean and std.dev: (1873.59, 2250.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10877) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.14, 89.82) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 66, 3099) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9261) [M::mem_pestat] mean and std.dev: (1724.18, 2730.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12647) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 944.899 CPU sec, 55.957 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 667020, 42, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (387, 1112, 1838) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4740) [M::mem_pestat] mean and std.dev: (980.92, 860.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6191) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.29, 90.00) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 46, 2812) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8394) [M::mem_pestat] mean and std.dev: (1127.37, 1722.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11185) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (358, 1218, 1609) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4111) [M::mem_pestat] mean and std.dev: (1020.60, 625.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5362) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 941.447 CPU sec, 42.143 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 666856, 48, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (293, 377, 1139) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2831) [M::mem_pestat] mean and std.dev: (624.08, 544.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3677) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.14, 89.95) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 527, 2782) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8284) [M::mem_pestat] mean and std.dev: (1428.15, 1787.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11035) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (792, 1052, 3339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8433) [M::mem_pestat] mean and std.dev: (2156.32, 2234.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11094) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 945.507 CPU sec, 54.911 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 666559, 39, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (289, 398, 566) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1120) [M::mem_pestat] mean and std.dev: (367.36, 129.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1397) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.02, 89.86) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 70, 1548) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4604) [M::mem_pestat] mean and std.dev: (727.76, 1227.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6132) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (380, 1069, 1951) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5093) [M::mem_pestat] mean and std.dev: (1111.72, 1032.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6664) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 944.246 CPU sec, 42.099 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 667296, 47, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (349, 848, 1507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3823) [M::mem_pestat] mean and std.dev: (795.38, 529.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4981) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (370, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (130, 730) [M::mem_pestat] mean and std.dev: (432.03, 90.11) [M::mem_pestat] low and high boundaries for proper pairs: (10, 850) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 60, 585) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1715) [M::mem_pestat] mean and std.dev: (165.95, 295.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2280) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (941, 1101, 1436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2426) [M::mem_pestat] mean and std.dev: (979.40, 285.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2921) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 945.381 CPU sec, 56.152 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 666713, 38, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (517, 1084, 1861) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4549) [M::mem_pestat] mean and std.dev: (1029.88, 779.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5893) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (370, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (130, 730) [M::mem_pestat] mean and std.dev: (432.10, 90.15) [M::mem_pestat] low and high boundaries for proper pairs: (10, 850) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 563, 2981) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8899) [M::mem_pestat] mean and std.dev: (1464.00, 1939.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11858) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 946.920 CPU sec, 42.112 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 667083, 40, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.11, 89.99) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 236, 2018) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6010) [M::mem_pestat] mean and std.dev: (973.65, 1361.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8006) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 945.452 CPU sec, 55.074 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 666828, 35, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (533, 793, 2781) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7277) [M::mem_pestat] mean and std.dev: (1473.50, 1650.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9525) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.21, 90.18) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 97, 2926) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8736) [M::mem_pestat] mean and std.dev: (1646.71, 2834.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12987) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 942.244 CPU sec, 42.059 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 666851, 33, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (321, 482, 1898) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5052) [M::mem_pestat] mean and std.dev: (912.08, 729.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6629) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.03, 89.94) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 92, 2712) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8086) [M::mem_pestat] mean and std.dev: (1105.48, 1942.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10773) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 949.434 CPU sec, 55.394 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 666822, 39, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (315, 829, 1422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3636) [M::mem_pestat] mean and std.dev: (765.25, 589.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4743) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.13, 89.94) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 73, 796) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2326) [M::mem_pestat] mean and std.dev: (281.12, 521.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3091) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1024, 1911, 4558) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11626) [M::mem_pestat] mean and std.dev: (2965.09, 2393.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15160) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 944.450 CPU sec, 42.078 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 666132, 56, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (354, 929, 1498) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3786) [M::mem_pestat] mean and std.dev: (839.09, 553.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4930) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.20, 90.05) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 50, 1355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4029) [M::mem_pestat] mean and std.dev: (385.27, 828.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5366) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (813, 3012, 6217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 17025) [M::mem_pestat] mean and std.dev: (3672.80, 3466.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 22429) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 953.976 CPU sec, 56.127 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666922, 26, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (414, 480, 2317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6123) [M::mem_pestat] mean and std.dev: (1223.27, 1054.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8026) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.16, 89.94) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 964, 2637) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7863) [M::mem_pestat] mean and std.dev: (1296.20, 1431.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10476) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 942.612 CPU sec, 42.088 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666077, 38, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (336, 357, 1250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3078) [M::mem_pestat] mean and std.dev: (512.33, 310.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3992) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (370, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (130, 730) [M::mem_pestat] mean and std.dev: (432.06, 90.03) [M::mem_pestat] low and high boundaries for proper pairs: (10, 850) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 464, 2861) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8531) [M::mem_pestat] mean and std.dev: (1625.81, 1946.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11366) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 948.496 CPU sec, 56.658 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 667186, 46, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (303, 517, 1602) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4200) [M::mem_pestat] mean and std.dev: (801.31, 697.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5499) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.20, 89.98) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 74, 1582) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4684) [M::mem_pestat] mean and std.dev: (990.45, 1526.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7098) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1020, 1333, 2562) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5646) [M::mem_pestat] mean and std.dev: (1882.08, 1379.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7400) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 942.122 CPU sec, 41.922 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 666994, 40, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (535, 996, 1949) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4777) [M::mem_pestat] mean and std.dev: (1309.46, 1067.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6191) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (370, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (130, 730) [M::mem_pestat] mean and std.dev: (432.12, 90.16) [M::mem_pestat] low and high boundaries for proper pairs: (10, 850) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 205, 2545) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7575) [M::mem_pestat] mean and std.dev: (1021.37, 1486.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10090) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (528, 1302, 3526) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9522) [M::mem_pestat] mean and std.dev: (1663.00, 1532.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12520) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 937.246 CPU sec, 52.512 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 666669, 41, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (354, 581, 1652) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4248) [M::mem_pestat] mean and std.dev: (771.76, 625.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5546) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.07, 89.85) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 48, 1790) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5334) [M::mem_pestat] mean and std.dev: (857.88, 1455.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7106) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (371, 764, 2061) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5441) [M::mem_pestat] mean and std.dev: (1014.69, 831.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7131) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 947.662 CPU sec, 42.065 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 666722, 35, 11) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 426, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (431.97, 89.99) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 43, 1315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3915) [M::mem_pestat] mean and std.dev: (588.94, 869.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5215) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (554, 1070, 2263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5681) [M::mem_pestat] mean and std.dev: (1312.36, 1002.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7390) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 944.270 CPU sec, 54.409 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 666490, 36, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (294, 471, 948) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2256) [M::mem_pestat] mean and std.dev: (535.27, 406.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2910) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.33, 90.20) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 75, 2577) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7671) [M::mem_pestat] mean and std.dev: (1137.19, 1777.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10218) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (930, 1260, 1970) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4050) [M::mem_pestat] mean and std.dev: (1165.86, 675.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5090) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 938.906 CPU sec, 41.551 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 666949, 33, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.07, 89.89) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 41, 2510) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7506) [M::mem_pestat] mean and std.dev: (777.26, 1347.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10004) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 941.506 CPU sec, 54.727 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 666806, 44, 11) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.30, 89.91) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 89, 2324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6916) [M::mem_pestat] mean and std.dev: (889.38, 1291.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9212) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (571, 1040, 2300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5758) [M::mem_pestat] mean and std.dev: (1316.36, 1078.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7487) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 940.285 CPU sec, 54.556 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 666450, 43, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (307, 790, 1666) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4384) [M::mem_pestat] mean and std.dev: (989.95, 871.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5743) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.13, 90.10) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 53, 1984) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5886) [M::mem_pestat] mean and std.dev: (876.71, 1510.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7837) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (314, 944, 1623) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4241) [M::mem_pestat] mean and std.dev: (1107.93, 970.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5550) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 939.037 CPU sec, 41.744 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 666763, 39, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (300, 438, 2064) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5592) [M::mem_pestat] mean and std.dev: (917.75, 1055.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7356) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (370, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (130, 730) [M::mem_pestat] mean and std.dev: (432.05, 90.11) [M::mem_pestat] low and high boundaries for proper pairs: (10, 850) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 88, 3041) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9071) [M::mem_pestat] mean and std.dev: (1574.31, 2194.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12086) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 940.536 CPU sec, 54.447 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 667462, 41, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (501, 781, 1714) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4140) [M::mem_pestat] mean and std.dev: (1051.31, 727.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5353) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.03, 89.84) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 61, 1785) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5295) [M::mem_pestat] mean and std.dev: (853.35, 1393.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7050) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (802, 1159, 1527) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2977) [M::mem_pestat] mean and std.dev: (1035.27, 528.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3702) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 933.581 CPU sec, 41.392 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 667084, 36, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (335, 922, 1783) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4679) [M::mem_pestat] mean and std.dev: (1215.29, 1108.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6127) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.14, 89.99) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 55, 1416) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4220) [M::mem_pestat] mean and std.dev: (288.03, 686.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5622) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (450, 1059, 1571) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3813) [M::mem_pestat] mean and std.dev: (994.67, 780.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4934) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 931.408 CPU sec, 52.723 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 666210, 39, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (468, 572, 1239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2781) [M::mem_pestat] mean and std.dev: (779.21, 463.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3552) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.12, 89.97) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 115, 2552) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7606) [M::mem_pestat] mean and std.dev: (974.14, 1396.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10133) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 940.771 CPU sec, 41.838 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 666887, 45, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (335, 709, 1698) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4424) [M::mem_pestat] mean and std.dev: (792.00, 601.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5787) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.05, 89.94) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 31, 4357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13045) [M::mem_pestat] mean and std.dev: (1630.87, 2485.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17389) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (866, 1382, 2332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5264) [M::mem_pestat] mean and std.dev: (1847.73, 1429.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7567) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 934.032 CPU sec, 52.065 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 666465, 39, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.43, 90.19) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (87, 887, 4332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12822) [M::mem_pestat] mean and std.dev: (1898.10, 2087.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17067) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 940.354 CPU sec, 41.828 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 666574, 47, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (361, 805, 4963) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14167) [M::mem_pestat] mean and std.dev: (2285.06, 2582.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18769) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.29, 90.17) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 46, 1950) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5802) [M::mem_pestat] mean and std.dev: (803.93, 1338.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7728) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 937.260 CPU sec, 53.081 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 666263, 40, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (331, 420, 822) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1804) [M::mem_pestat] mean and std.dev: (440.27, 269.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2295) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 731) [M::mem_pestat] mean and std.dev: (432.29, 90.21) [M::mem_pestat] low and high boundaries for proper pairs: (11, 851) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 61, 3157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9441) [M::mem_pestat] mean and std.dev: (1408.40, 2205.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12583) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 932.907 CPU sec, 41.307 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 666836, 40, 13) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.05, 89.75) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 83, 2594) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7742) [M::mem_pestat] mean and std.dev: (928.97, 1447.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10316) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (468, 845, 2785) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7419) [M::mem_pestat] mean and std.dev: (1087.67, 834.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9736) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 938.216 CPU sec, 52.578 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 667054, 38, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (333, 575, 1550) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3984) [M::mem_pestat] mean and std.dev: (1048.86, 1019.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5201) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (371, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 728) [M::mem_pestat] mean and std.dev: (432.02, 90.01) [M::mem_pestat] low and high boundaries for proper pairs: (14, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 39, 494) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1450) [M::mem_pestat] mean and std.dev: (133.58, 307.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1928) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 930.887 CPU sec, 41.206 real sec [M::process] read 1221144 sequences (183171600 bp)... [M::process] 0 single-end sequences; 1221144 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 508697, 43, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (370, 427, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (130, 730) [M::mem_pestat] mean and std.dev: (432.17, 89.97) [M::mem_pestat] low and high boundaries for proper pairs: (10, 850) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 97, 2851) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8507) [M::mem_pestat] mean and std.dev: (1608.24, 2078.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11335) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1221144 reads in 720.034 CPU sec, 30.480 real sec [main] Version: 0.7.17-r1188 [main] CMD: /home/csyk2proj/tools/bwa.kit/bwa mem -p -t24 -R@RG\tID:KPGP-00363\tPL:ILLUMINA\tSM:KPGP-00363 -C /home/csyk2proj/data/bwa.kit/hs38DH.fa - [main] Real time: 49247.890 sec; CPU: 558586.532 sec