| identifier |
PRJDB12336 |
| type |
bioproject |
| sameAs |
|
| organism |
Escherichia coli BW25113
|
| title |
Ribosome profiling analyses on WT and RRFTs strain |
| description |
In the early stage of translation elongation, peptidyl-tRNA (pep-tRNA) with short nascent chain frequently dissociates from the ribosome (pep-tRNA drop-off). Inactivation of PTH (peptidyl-tRNA hydrolase) causes rapid accumulation of pep-tRNAs and depletion of aminoacyl(aa)-tRNAs, leading to defective protein synthesis. We established a highly sensitive method for direct profiling of pep-tRNAs accumulated in the cell and identified more than 700 peptide sequences of the pep-tRNAs. In this experiment, to examine the involvement of RRF in pep-tRNA drop-off in the early elongation stage, we performed ribosome profiling of WT and temperature-sensitive RRF strain incubated at 43°C for 30 min. |
| data type |
Transcriptome or Gene Expression
|
| publication |
|
| properties ▽ |
{...}
|
| dbXrefs |
|
| distribution |
JSONJSON-LD
|
| Download |
|
| status |
public |
| visibility |
unrestricted-access |
| dateCreated |
2021-09-29T02:32:52+09:00 |
| dateModified |
2023-05-18T13:01:02+09:00 |
| datePublished |
2023-05-18T13:01:02+09:00 |