| description |
The 'true causative bacteria of the abscess' can be difficult to detect due to prior antimicrobial agents, as culture specimens may be collected after treatment has started. Recent innovative genome sequencing technology, which is referred to as next-generation sequencing (NGS), enables analyses of bacterial flora using 16S ribosomal DNA. NGS is frequently used to detect true pathogenic bacteria in abscesses and can also detect dead bacteria if 16S ribosomal DNA remains, leading to identification of pathogenic bacteria after antibiotic therapy. This project included patients with fallopian tube abscess, ovarian abscess, infectious lymphoceles after treatments for gynecological malignancies, pyometra uteri and postoperative Douglas cysts. To determine the true causative bacteria and to seek knowledge that will provide the basis for new prevention and treatment strategies. |