home > bioproject > PRJDB1821
identifier PRJDB1821
type bioproject
sra-study  DRP001030
organism Ralstonia eutropha H16
title RNAseq_ReutH16
description Ralstonia eutropha H16 is well-known to produce poly(3-hydroxybutyrate) [P(3HB)], a kind of polyhydroxyalkanoates attracted as bio-based biodegradable plastics, efficiently as an energy storage material under unbalanced growth conditions. To obtain further extended knowledge of PHA biosynthesis, this study employed quantitative transcriptome analysis based on deep sequencing of complementary DNA generated from RNA (RNA-seq) for R. eutropha H16.Total RNAs were extracted from R. eutropha cells in growth, PHA production, and stationary phases on fructose. rRNAs in the preparation were removed by repeated treatments with magnetic beads specific to bacterial rRNAs, and then the 36 bp sequences were determined by using Illumina high-throughput sequencer.The RNA-seq results supported induction of gene expression for transcription, translation, cell division, peptidoglycan biosynthesis, pilus and flagella assembly, energy conservation, and fatty acid biosynthesis in growth phase, and repression trends for genes involved in central metabolisms in PHA production phase.
data type Other
external link
sra-run  DRR008642DRR008643
sra-submission  DRA000990
biosample  SAMD00002597
sra-study  DRP001030
sra-sample  DRS007666
sra-experiment  DRX007770DRX007771
distribution JSONJSON-LD
status public
visibility unrestricted-access
dateCreated 2014-05-12T00:46:27+0000
dateModified 2015-05-11T00:50:37+0000
datePublished 2015-05-11T00:50:37+0000