home > bioproject > PRJDB2065
identifier PRJDB2065
type bioproject
sra-study  DRP000929
organism Homo sapiens
title Human inactive X chromosome is compacted through a polycomb-independent SMCHD1-HBiX1 pathway [RNA-seq]
description Human inactive X chromosome (Xi) forms a compact structure called the Barr body, which is enriched in repressive histone modifications such as trimethylation of histone H3 Lys9 (H3K9me3) and Lys27 (H3K27me3). These two histone marks are distributed in distinct domains, and XIST (X inactive specific transcript) preferentially colocalizes with H3K27me3 domains. Here, we show that Xi compaction requires HBiX1, a heterochromatin protein 1 (HP1)–binding protein, and SMCHD1 (structural maintenance of chromosomes hinge domain containing 1), both of which are enriched throughout the Xi chromosome. HBiX1 localization to H3K9me3 and XIST-associated H3K27me3 (XIST-H3K27me3) domains was mediated through interactions with HP1 and SMCHD1, respectively. Furthermore, HBiX1 was required for SMCHD1 localization to H3K9me3 domains. Depletion of HBiX1 or SMCHD1, but not Polycomb repressive complex 2 (PRC2), resulted in Xi decompaction, similarly to XIST depletion. Thus, the molecular network involving HBiX1 and SMCHD1 links the H3K9me3 and XIST-H3K27me3 domains to organize the compact Xi structure.
data type DDBJ SRA Study
external link
sra-run  DRR003595DRR003596DRR003597DRR003598
sra-submission  DRA000894
biosample  SAMD00006326SAMD00006334SAMD00006327SAMD00006333
sra-study  DRP000929
sra-sample  DRS003051DRS003052DRS003053DRS003054
sra-experiment  DRX002919DRX002920DRX002921DRX002922
distribution JSONJSON-LD
status public
visibility unrestricted-access
dateCreated 2014-05-12T00:46:27+0000
dateModified 2014-05-12T00:46:27+0000
datePublished 2014-05-12T00:46:27+0000