| description |
Themost important proteins involved in olfaction include odorant binding protein (OBP), chemosensory protein (CSP), olfactoryreceptor (OR), and gustatory receptor (GR). Despite that the exhaustive genomic analysis has revealed a large number of olfactorygenes in a number of model insects, it is still poorly understood for most nonmodel species. This is mostly due to the reason thatthe small antenna is challenging for collection. We can generally isolate one or few genes at a time by means of the traditionalmethod. Here, we present the large-scale identifying members of the main olfactory genes from the head of Tomicus yunnanensisusing Illumina sequencing. In a single run, we obtained over 51.8 million raw reads. These reads were assembled into 57,142unigenes. Nearly 29,384 of them were functionally annotated in the NCBI nonredundant database. By depth analysis of the data,11OBPs, 8 CSPs, 18ORs, and 8GRs were retrieved. Sequences encoding full length proteins were further characterised for oneOBP and two CSPs. The obtained olfactory genes provide a major resource in further unraveling the molecular mechanisms ofT. yunnanensis chemoperception. This study indicates that the next generation sequencing is an attractive approach for efficientidentification of olfactory genes from insects, for which the genome sequence is unavailable. |