home > bioproject > PRJDB2172
identifier PRJDB2172
type bioproject
sameAs
sra-study  DRP000464
organism Homo sapiens
title pre-miRNA profiles obtained through application of locked nucleic acids reveals complex 5’/3’ arm variation including concomitant cleavage and polyuridylation patterns
description Recent research hints at an underappreciated complexity in pre-miRNA processing and regulation. Global profiling of pre-miRNA and its potential to increase understanding of the pre-miRNA landscape is impeded by overlap with highly-expressed classes of other non-coding RNA. Here we present a dataset excluding these RNA before sequencing through locked nucleic acids (LNA), greatly increasing pre-miRNA sequence counts with no discernable effects on pre-miRNA or mature miRNA sequencing. Analysis of profiles generated in total, nuclear, and cytoplasmic cell fractions reveals pre-miRNAs are subject to a wide range of regulatory processes involving loci-specific 3’- and 5’-end variation entailing complex cleavage patterns with co-occurring polyuridylation. Additionally, examination of nuclear-enriched flanking sequences of pre-miRNA, particularly those derived from polycistronic miRNA transcripts, provides insight into miRNA and miRNA-offset (moRNA) production. Our findings point to particularly intricate regulation of the let-7 family, introduce novel and unify known forms of pre-miRNA regulation and processing, and shed new light on the byproducts of miRNA processing pathways. none provided
data type DDBJ SRA Study
publication
22058130
external link
properties 
{...}
dbXrefs
sra-run  DRR001482DRR001483DRR001484DRR001485DRR001486DRR001487DRR001488DRR001489
sra-submission  DRA000455
biosample  SAMD00009013SAMD00009015SAMD00009011SAMD00009018SAMD00009014SAMD00009017SAMD00009012SAMD00009016
sra-study  DRP000464
sra-sample  DRS001018DRS001019DRS001020DRS001021DRS001022DRS001023DRS001024DRS001025
sra-experiment  DRX001036DRX001037DRX001038DRX001039DRX001040DRX001041DRX001042DRX001043
distribution JSONJSON-LD
status public
visibility unrestricted-access
dateCreated 2014-05-12T00:46:27+0000
dateModified 2014-05-12T00:46:27+0000
datePublished 2014-05-12T00:46:27+0000