home > bioproject > PRJDB2174
identifier PRJDB2174
type bioproject
sameAs
sra-study  DRP000444
organism Hepatitis B virus
title Ultra-deep sequencing of hepatitis B virus quasispecies in association with clinical status and nucleos(t)ide analogue treatment
description Background & Aims: Although the advent of ultra-deep sequencing technology allows for the analysis of heretofore undetectable minor viral mutants, a limited amount of information is currently available regarding the clinical implications of hepatitis B virus (HBV) genomic heterogeneity. Methods: To characterize the HBV genetic complexity in association with various clinical settings, we performed ultra-deep sequencing of full-genome HBV in the liver of 23 liver transplant recipients, including 4 acute, 14 chronic-naïve, and 5 chronic nucleos(t)ide analogue (NA)-treated cases. Dynamic changes of the G1896A pre-core mutant were examined in the serum of 22 chronic-naïve non-transplant patients before and after entecavir administration.Results: The overall viral complexity was significantly lower in acute than chronic cases. Four of eight (50%) HBeAg-negative cases showed a substantially low prevalence of the G1896A pre-C mutant in the liver tissues, suggesting that other mutations were involved in their HBeAg seroconversion. Interestingly, liver tissues in 4 of 5 (80%) of the anti-HBe-positive cases receiving NA had extremely low levels of the G1896A pre-C mutant (0.0%, 0.0%, 0.1%, and 1.1%). Consistently, dynamic change analysis of the G1896A pre-C mutant demonstrated that entecavir administration significantly reduced the proportion of the G1896A pre-C mutant in 16 of 22 cases, irrespective of their HBeAg/Ab serostatus, suggesting the high sensitivity of the G1896A pre-C mutant to NA.Conclusions: Our findings illustrate the strong advantage of deep sequencing as a tool for dissecting the pathophysiology of HBV infection and provide a rationale for future application of this technology to predict the therapy outcome. none provided
data type DDBJ SRA Study
publication
22523569
external link
properties 
{...}
dbXrefs
sra-run  DRR001331DRR001332DRR001333DRR001334DRR001335DRR001336DRR001337DRR001338DRR001339DRR001340 More
sra-submission  DRA000435
biosample  SAMD00009037SAMD00009034SAMD00009023SAMD00009028SAMD00009043SAMD00009044SAMD00009033SAMD00009032SAMD00009025SAMD00009024 More
sra-study  DRP000444
sra-sample  DRS000826DRS000827DRS000828DRS000829DRS000830DRS000831DRS000832DRS000833DRS000834DRS000835 More
sra-experiment  DRX000928DRX000929DRX000930DRX000931DRX000932DRX000933DRX000934DRX000935DRX000936DRX000937 More
distribution JSONJSON-LD
status public
visibility unrestricted-access
dateCreated 2014-05-12T00:46:27+0000
dateModified 2014-05-12T00:46:27+0000
datePublished 2014-05-12T00:46:27+0000