home > bioproject > PRJDB2257
identifier PRJDB2257
type bioproject
sra-study  DRP000980
organism Bombyx mori
title Hsp90 facilitates accurate loading of precursor piRNAs into PIWI proteins
description PIWI-interacting RNAs (piRNAs) defend the genome against transposon activity in animal gonads. The Hsp90 chaperone machinery has been implicated in the piRNA pathway, but its exact role remains obscure. Here, we examined the effect of 17-N-allylamino-17-demethoxygeldanamycin (17-AAG), an Hsp90-specific inhibitor, on the piRNA pathway. In the silkworm ovary-derived BmN4 cells, 17-AAG treatment reduced the level of piRNAs and PIWI proteins. This was accompanied with (1) reducation of 1U and 10A piRNAs in total piRNA populaton and (2) loosened preference for 1U and 10 piRNAs of Siwi and BmAgo3, respectively.
data type DDBJ SRA Study
external link
sra-run  DRR006265DRR006266DRR006267DRR006268DRR006269DRR006270
sra-submission  DRA000943
biosample  SAMD00009603SAMD00009604SAMD00009607SAMD00009605SAMD00009606SAMD00009602
sra-study  DRP000980
sra-sample  DRS005628DRS005629DRS005630DRS005631DRS005632DRS005633
sra-experiment  DRX005489DRX005490DRX005491DRX005492DRX005493DRX005494
distribution JSONJSON-LD
status public
visibility unrestricted-access
dateCreated 2014-05-12T00:46:27+0000
dateModified 2014-05-12T00:46:27+0000
datePublished 2014-05-12T00:46:27+0000