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identifier PRJDB2257
type bioproject
sra-study  DRP000980
organism Bombyx mori
title Hsp90 facilitates accurate loading of precursor piRNAs into PIWI proteins
description PIWI-interacting RNAs (piRNAs) defend the genome against transposon activity in animal gonads. The Hsp90 chaperone machinery has been implicated in the piRNA pathway, but its exact role remains obscure. Here, we examined the effect of 17-N-allylamino-17-demethoxygeldanamycin (17-AAG), an Hsp90-specific inhibitor, on the piRNA pathway. In the silkworm ovary-derived BmN4 cells, 17-AAG treatment reduced the level of piRNAs and PIWI proteins. This was accompanied with (1) reducation of 1U and 10A piRNAs in total piRNA populaton and (2) loosened preference for 1U and 10 piRNAs of Siwi and BmAgo3, respectively.
data type DDBJ SRA Study
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