home > bioproject > PRJDB2455
identifier PRJDB2455
type bioproject
sra-study  DRP000840
organism Oryza sativa Japonica Group
title QTL-seq: Rapid mapping of quantitative trait loci in rice by whole genome resequencing of two bulked DNA
description The majority of agronomically important crop traits are quantitative, meaning that they are controlled by multiple genes each with a small effect (quantitative trait loci: QTL). QTL mapping and isolation is important for efficient crop breeding by marker-assisted selection (MAS) and for a better understanding of the molecular mechanisms underlying the traits. Since it requires the development and selection of DNA markers for linkage analysis, QTL analysis is however time consuming and labor intensive. Here we propose “QTL-seq”, a method for rapid identification of QTL by whole genome resequencing of two bulked DNAs of individuals showing extreme opposite extreme trait values for a given phenotype in a segregating progeny. We applied QTL-seq to rice recombinant inbred lines (RILs) and F2 populations and successfully identified QTL for important agronomic traits, such as partial resistance to the fungal rice blast disease and seedling vigor. Simulation study showed that QTL-seq is able to detect QTL over wide ranges of experimental variables, and the method can be generally applied in population genomics studies to rapidly identify genomic regions that underwent artificial or natural selective sweeps. none provided
data type DDBJ SRA Study
external link
sra-run  DRR003237DRR003238DRR003239DRR003240DRR003337DRR003338DRR003339DRR003340DRR003341DRR003342 More
sra-submission  DRA000809
biosample  SAMD00011906SAMD00011902SAMD00011903SAMD00011905SAMD00011908SAMD00011904SAMD00011907SAMD00011909SAMD00011910SAMD00011901
sra-study  DRP000840
sra-sample  DRS002673DRS002674DRS002675DRS002676DRS002755DRS002756DRS002757DRS002758DRS002759DRS002760
sra-experiment  DRX002569DRX002570DRX002571DRX002572DRX002660DRX002661DRX002662DRX002663DRX002664DRX002665
distribution JSONJSON-LD
status public
visibility unrestricted-access
dateCreated 2014-05-12T00:46:27+0000
dateModified 2014-05-12T00:46:27+0000
datePublished 2014-05-12T00:46:27+0000