home > bioproject > PRJDB2651
identifier PRJDB2651
type bioproject
sra-study  DRP000436
organism Mus musculus
title 454 libraries of PCR products for T cell receptors in regulatory and conventional CD4-expressing T lymphocytes
description Fractions of the T cell repertoire of wild type (WT) mice or mice engineered for fixing their TCR β chain by somatic cloning (1D2b) or transgenesis (Vb3Tg) were characterised by sequencing α or β full clonotypes on the 454 GS FLX Titanium platform, after amplifying TCRs by PCR with a forward primer specific of a family of V segments, and a reverse primer specific of the C segment. Conventional and regulatory T cells were separated by flow-sorting according to their expression of Foxp3, detected through the expression of the human CD2 protein (hCD2) in a hCD2 Foxp3 knock-in background. none provided
data type Other
external link
sra-run  DRR001199DRR001200DRR001201DRR001202DRR001203DRR001204DRR001205DRR001206DRR001207DRR001208 More
sra-submission  DRA000428
biosample  SAMD00013521SAMD00013536SAMD00013538SAMD00013530SAMD00013525SAMD00013539SAMD00013529SAMD00013519SAMD00013523SAMD00013524 More
sra-study  DRP000436
sra-sample  DRS000760DRS000764DRS000763DRS000766DRS000761DRS000762DRS000765DRS000767DRS000768DRS000769 More
sra-experiment  DRX000796DRX000800DRX000802DRX000803DRX000797DRX000798DRX000799DRX000801DRX000804DRX000805 More
distribution JSONJSON-LD
status public
visibility unrestricted-access
dateCreated 2014-05-12T00:46:27+0000
dateModified 2017-12-14T13:09:57+0000
datePublished 2017-12-14T13:09:57+0000