| description |
The current knowledge on the effect of global climate change on paddy CH4 emission is incomplete, partly because of the limited information about the response of the latter to elevated ground-level ozone (O3), one important element of global climate change, and the related microbial mechanism. In this study, we carried out a field experiment in the China Ozone Free-Air Concentration Enrichment (FACE-O3) facility in a rice-wheat rotation system. Responses of methanogenic archaeal community composition and diversity to elevated ground-level O3 at rice tillering and anthesis stages, were analyzed by 454 pyrosequencing; both population and taxa-specific sizes were evaluated with real-time quantitative PCR and the methanogenic activity was determined by measuring CH4 production during microcosm incubation. We found that elevated ground-level O3 inhibited the methanogenic activity and potentially influenced paddy methanogenic community composition and reduced their abundance and diversity through adversely affecting the dominant groups, such as aceticlastic Methanosaeta, especially at rice tillering stage. Our results indicate that a continuously elevated ground-level O3 would negatively influence methanogenic archaeal community and its involved ecological function. These findings will contribute to the comprehensive understanding of responses and feedbacks of paddy ecosystem to global climate change. |