| identifier |
PRJDB2919 |
| type |
bioproject |
| sameAs |
|
| organism |
Mus musculus
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| title |
Objective molecular criteria for oocyte quality and developmental potential: a proteomic study in inbred mice |
| description |
Current understanding of oocyte quality and developmental potential maintains that stochastic or epigenetic processes modulate the action of determinants that are laid in the oocyte during oogenesis. These determinants are considered to be rather conserved across mice, leading different strains of mice to be used interchangeably. We challenged this assumption and studied the relationship between oocyte composition and developmental quality in four imbred strains of mice, namely 129Sv, C57Bl/6, C3H/HeN and DBA/2J. These oocytes showed large variability developmental competence and embryo quality irrespective of the developmental stimulus (fertilization, somatic cell nuclear transfer, parthenogenesis). To unravel the molecular basis of the observed phenotypes we applied state-of-the-art preoteomics (SILAC LC-MS/MS) combined with transcriptomics (RNA deep sequencing). We quantified 1839 proteins and 20413 transcripts simultaneously in oocytes of all four strains. The proteome and the transcriptome had little correlation with each other, highlighting the importance of proteomic quantifications in embryology. We found that proteins that were most variably expressed between oocytes from different strains mainly relate to oocyte biology, ribosome and RNA biogenesis as well as embryo differentiation and chromatin remodelling. Thus, different strains of mice should not be used interchangeably in biology when tackling questions about oogenesis and early development. |
| data type |
Transcriptome or Gene Expression
|
| publication |
|
| properties ▽ |
{...}
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| dbXrefs |
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| distribution |
JSONJSON-LD
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| Download |
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| status |
public |
| visibility |
unrestricted-access |
| dateCreated |
2014-06-16T01:41:40+09:00 |
| dateModified |
2016-06-16T13:09:59+09:00 |
| datePublished |
2016-06-16T13:09:59+09:00 |