home > bioproject > PRJDB3159
identifier PRJDB3159
type bioproject
sameAs
organism Synechocystis sp. PCC 6803
title Genomic analysis of parallel-evolved cyanobacterium Synechocystis sp. PCC 6803 under acid stress
description Experimental evolution is a powerful tool for clarifying phenotypic and genotypic changes responsible for adaptive evolution. In this study, we isolated acid-acclimated Synechocystis sp. PCC 6803 strains to identify genes involved in acid tolerance. Synechocystis 6803 are rarely found in habitants with pH < 5.75. The parent strain was cultured in BG-11 at pH 6.0. We gradually lowered the pH of the medium from pH 6.0 to pH 5.5 over 3 months. Our acclimated cells could grow in acid stress conditions at pH 5.5, whereas the parent cells could not. We performed whole-genome sequencing and compared the acid-acclimated and parent strains, thereby identifying 11 SNPs in the acid-acclimated strains. These results suggest that SNPs in certain genes are involved in acid stress tolerance in Synechocystis 6803.
data type Genome Sequencing
publication
properties 
{...}
dbXrefs
sra-run  DRR023149DRR023150DRR023151
sra-submission  DRA002534
biosample  SAMD00020274SAMD00020275SAMD00020276
sra-study  DRP002598
sra-sample  DRS019291DRS019292DRS019293
sra-experiment  DRX021203DRX021204DRX021205
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status public
visibility unrestricted-access
dateCreated 2014-09-21T02:24:23+09:00
dateModified 2015-03-19T00:29:09+09:00
datePublished 2015-03-19T00:29:09+09:00