identifier |
PRJDB3159 |
type |
bioproject |
sameAs |
|
organism |
Synechocystis sp. PCC 6803
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title |
Genomic analysis of parallel-evolved cyanobacterium Synechocystis sp. PCC 6803 under acid stress |
description |
Experimental evolution is a powerful tool for clarifying phenotypic and genotypic changes responsible for adaptive evolution. In this study, we isolated acid-acclimated Synechocystis sp. PCC 6803 strains to identify genes involved in acid tolerance. Synechocystis 6803 are rarely found in habitants with pH < 5.75. The parent strain was cultured in BG-11 at pH 6.0. We gradually lowered the pH of the medium from pH 6.0 to pH 5.5 over 3 months. Our acclimated cells could grow in acid stress conditions at pH 5.5, whereas the parent cells could not. We performed whole-genome sequencing and compared the acid-acclimated and parent strains, thereby identifying 11 SNPs in the acid-acclimated strains. These results suggest that SNPs in certain genes are involved in acid stress tolerance in Synechocystis 6803. |
data type |
Genome Sequencing
|
publication |
|
properties ▽ |
{...}
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dbXrefs |
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distribution |
JSONJSON-LD
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Download |
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status |
public |
visibility |
unrestricted-access |
dateCreated |
2014-09-21T02:24:23+09:00 |
dateModified |
2015-03-19T00:29:09+09:00 |
datePublished |
2015-03-19T00:29:09+09:00 |