| identifier |
PRJDB3416 |
| type |
bioproject |
| sameAs |
|
| organism |
Homo sapiens
|
| title |
Single-cell RNA-seq analysis of the cell cycle |
| description |
The goal of these libraries is to find transcriptome signatures of the
different phases of the cell cycle. Single HeLa cells expressing
fluorescent reporters that peak in G1 and G2/M phases were captured in
C1 Single-Cell Auto Prep systems (Fluidigm recording red and green
fluorescence for each cell individually, each cell was lysed and their
RNAs converted to cDNAs in the C1 systems using SMARTer kits (Clontech).
The cDNAs were then collected and converted to RNA-seq libraries by
tagmentation using Illumina/Nextera kits. The cells used in this
experiment are unpublished as of today, and precise details will be
given later, together with the fluorescence values. In the meantime,
this dataset can be used to assess reproducibility of single-cell
RNA-seq over 5 runs in the Fluidigm system. |
| data type |
Transcriptome or Gene Expression
|
| publication |
|
| properties ▽ |
{...}
|
| dbXrefs |
|
| distribution |
JSONJSON-LD
|
| Download |
|
| status |
public |
| visibility |
unrestricted-access |
| dateCreated |
2014-12-26T08:21:26+09:00 |
| dateModified |
2014-12-26T09:05:19+09:00 |
| datePublished |
2014-12-26T08:54:33+09:00 |