| description |
The raw sequences, obtained from illumina MiSeq sequencing, were processed (e.g. trimming, denoising, chimeras removing, etc.). A total of 170098 effective sequences from all (fermentation pit mud) FPM samples remained and the number of reads per sample ranged from 13613 to 14653. Based on 97% similarity of 16S rRNA gene sequences, they distributed into 4927 OTUs and each sample contained 774 to 1453 OTUs. Moreover, the coverage of effective reads per sample was higher than 94% (ranged from 94.3% to 96.4%).For the general phylogenetic composition of FPM community, a total of 33 phyla including 31 bacterial and 2 archaeal phyla, with relative abundance per sample ranged from 90.96-98.44%, were observed. Among them, 20 phyla were commonly shared by all of the FPMs, with abundances accounted for 89.21-98.34%. The dominant phyla (>10%, of total effective sequences) were Firmicutes (58%), Euryarchaeota (19%) and Bacteroidetes (11%). |