home > bioproject > PRJDB3866
identifier PRJDB3866
type bioproject
sameAs
organism Homo sapiens
title Transcriptome Sequencing and Comparative Analysis of Two Types of Exosomes in Human Whole Saliva by Next Generation Sequencing
description Exosomes are small membrane vesicles (30-100 nm in diameter) secrted by numerous cells. Exosome have been shown to contain mRNA and DNA. We found at least two types of salivary exosomes (exosome I and exosome II) that are different in size and have different proteome. In previous study, we performed small RNA transcriptome analysis by next generation sequencing technology and reported that many types of small RNA, such as miRNA, piwi-interacting RNA (piRNA), small nucleolar RNA (snoRNA), other small RNAs and genomic repeats were contained in exosome I, II and whole saliva. In this study, we performed whole-transcriptome analysis using cDNA libraries constructed from total RNA except small RNAs. We found that 11-32% of reads from salivary exosomes and whole saliva were mapped to pseudogene. The Data Access Committee of the National Bioscience Database Center (NBDC) approved that this personal genetic data were made published according to NBDC data sharing guidelines (http://humandbs.biosciencedbc.jp/).
data type Transcriptome or Gene Expression
publication
properties 
{...}
dbXrefs
sra-run  DRR033935DRR033936DRR033937
sra-submission  DRA003516
biosample  SAMD00012851SAMD00012852SAMD00012853
sra-study  DRP002625
sra-sample  DRS005865DRS005866DRS005867
sra-experiment  DRX030655DRX030656DRX030657
distribution JSONJSON-LD
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status public
visibility unrestricted-access
dateCreated 2014-10-31T05:33:36+09:00
dateModified 2015-06-30T08:28:28+09:00
datePublished 2015-04-28T00:19:57+09:00