| identifier |
PRJDB3869 |
| type |
bioproject |
| sameAs |
|
| organism |
groundwater metagenome
|
| title |
Metabolically Active Bacteria of Nitrospirae Phylum Dominant in Deep Terrestrial Crust |
| description |
The terrestrial crust composed of granite is one of the largest, but least-understood, microbial habitats, because it is technically difficult to collect microbiological samples without contamination. To reduce contamination, short underground drilling was conducted in the deep granitic basement at the Mizunami underground research laboratory in Japan. Immediately after multi-packer installation, groundwater microbes were phylogenetically analyzed by Sanger sequencing of 16S rRNA gene and monitored over four years. After three years, phylotypes represented by one Nitrospirae clade were stably dominant. Pyrosequencing of 16S rRNA gene from groundwater microbes in a 25-year-old underground borehole in the deep granitic basement at the Grimsel Test Site in Switzerland revealed the dominance of bacteria also represented by the Nitrospirae clade. The Nitrospirae clade formed a firm cluster with environmental sequences from hot crustal fluids rather than those from the land surface. One-week incubation of the Mizunami groundwater with 13C-labeled bicarbonate and 1% H2 and subsequent nanometer-scale secondary ion MS demonstrated the abundance of microbial cells that mediate H2-dependent CO2 fixation. It is most likely that the Nitrospirae bacteria are widespread and playing an important role in carbon cycling in the deep terrestrial crust. |
| data type |
Metagenome
|
Targeted Locus (Loci)
|
| publication |
|
| properties ▽ |
{...}
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| dbXrefs |
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| distribution |
JSONJSON-LD
|
| Download |
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| status |
public |
| visibility |
unrestricted-access |
| dateCreated |
2015-04-22T12:43:07+09:00 |
| dateModified |
2016-05-31T13:03:13+09:00 |
| datePublished |
2016-05-31T13:03:13+09:00 |