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The first draft of common Marmoset (Callithrix jacchus) genome was published by the Marmoset Genome Sequencing and Analysis Consortium (MGSAC) in collaboration with the Baylor College of Medicine, and the Genome Sequencing Center at Washington University, St. Louis. The draft was based on whole genome shotgun sequencing, and the current assembly version is Callithrix_jacches-3.2.1. Callithrix_jacches-3.2.1 is mostly (95%) mapped to chromosomes, but still made of supercontigs (N50 of 6.7Mbp) and relatively short contigs (N50 of 30Kbp). The estimated genome size is about 3.0Gbp.
We performed resequencing and assembling the common marmoset genome being bred by the Central Institute for Experimental Animals (CIEA) in Japan. The marmoset genome was sequenced by deep sequencing with high-throughput sequencing technology. Several different sequence runs using Illumina sequencing platforms were executed, and 181Gbp of high quality bases including mate-pairs with several different long insert lengths (3Kbp, 8Kbp, 20Kbp and 40Kbp) were obtained, that is, roughly 60x coverage.
The resequence significantly improved the MGSAC draft genome sequence. The statistical measure N50 of contigs to evaluate the assembly quality increased to double. As the result, 80% of contigs (total length: 106Mbp) that have remained unmapped to chromosomes in the MGSAC draft were merged to contigs in chromosomes. |