description |
Nuclear genome of two Neolithic Japanese hunter-gatherers, Sanganji Jomons, were sequenced. The genetic origin of Jomon people and the genetic relationship between modern East Eurasians are not well understand, and clarifying those questions is important for understanding the history of East Eurasians. Mitochondrial DNA of Jomon people was well studied, and this give direct evidence of Jomon genotype. However, mitochondrial DNA is a single non-recombining molecule and carries limited genetic information. Therefore, the analysis of Jomon nuclear genome is necessary. From two individuals (sample A and sample B), three DNA libraries (A1, A2, and B) were prepared, and frequency of contaminations were inferred from mitochondrial DNA Capture method. Shotgun sequences were also conducted with Illumina GAIIx and Hiseq 2000. |