description |
Most of the terraced rice-fields in the hilly and mountainous area at Nuta, Otoyo in Kochi, Japan, were abandoned or have been changed to citron fields, forest lands, and bamboo thickets. Soil bacterial community structures of these different land usages were analyzed using 16S rRNA partial gene sequences. DGGE band patters of each soil were quite similar, although several paddy-field specific DNA bands were observed. Based on pyrosequencing data, bacterial species richness was slightly higher in paddy fields than in other soils. While overall bacterial community structures of soils at different land usages were similar, beta-diversity analysis clearly indicated that there are three clusters. Detail bacterial community structure of paddy fields was different from those of non-paddy field land usages. Interestingly, community structure of crop fields, which have not been used as paddy field, was different from those of at least one-time paddy fields. This might reflect the history of land usage. |