home > bioproject > PRJDB4247
identifier PRJDB4247
type bioproject
sameAs
organism Mus musculus
title RNA sequencing project of mouse
description E13.5 Atm+/- pregnant mice were intraperitoneally injected with saline or etoposide 0.5 mg/kg consecutive three days, and scarified 24 hr after final injection. After genotyping, samples were subjected RNA sequencing. Total RNA was extracted from mice fetal liver. The libraries were then submitted for Illumina HiSeq1500 sequencing according to the standard operation. Paired-end 90 or 100 nucleotide reads were generated and checked for data quality using FASTQC. After etoposide treatment, various chimeric fusion mRNAs were observed in Atm wild type and deficient liver cells. The number of chimeric fusion mRNAs was much higher in Atm deficient fetal liver than wild type littermate. Mll-ptp4a2 fusion mRNA was identified from Atm deficient liver cells by etoposide exposure.
data type Transcriptome or Gene Expression
publication
properties 
{...}
dbXrefs
sra-run  DRR046903DRR046904DRR046905DRR046906
sra-submission  DRA004049
biosample  SAMD00041600SAMD00041601SAMD00041602SAMD00041603
sra-study  DRP002785
sra-sample  DRS021436DRS021437DRS021438DRS021439
sra-experiment  DRX042191DRX042192DRX042193DRX042194
distribution JSONJSON-LD
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ddbj_core_bioproject.xml  HTTPS FTP
status public
visibility unrestricted-access
dateCreated 2015-09-19T12:49:42+09:00
dateModified 2015-09-24T07:54:25+09:00
datePublished 2015-09-24T07:54:25+09:00