| identifier |
PRJDB5612 |
| type |
bioproject |
| sameAs |
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| organism |
Oryzias latipes
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| title |
Transcriptome analysis during the process of degeneration and sex reversal of ovary in cyp19a1 mutant medaka |
| description |
Although estrogens have been generally considered to play a critical role in ovarian differentiation in non-mammalian vertebrates, the specific functions of estrogens during ovarian differentiation and development remain unclear. In this study, we isolated mutants medaka (Oryzias latipes) with premature stops in the ovarian aromatase (cyp19a1).
In these XX mutants, gonads first differentiated into normal ovaries containing many ovarian follicles. Subsequently, ovarian tissues underwent extensive degeneration, followed by the appearance of testicular tissues on the dorsal side of the ovarian cavity.
To identify the genes potentially involved in the process of degeneration and sex reversal of adult ovary in the ovarian aromatase mutant, we carried out RNAseq analysis.We used three sets of ovarian RNA samples for Illumina sequencing during the degeneration of ovaries in cyp19a1 mutants. Phase 1 ovaries were sampled before degeneration of the ovary commenced (n=8); Phase 2 ovaries were those in which ovarian follicles were degenerating (thick granulosa cell layer), but the external phenotype (morphology of fin) was female-type (n=4); and Phase 3 ovaries contained degenerating ovarian follicles and the fish displayed a male external phenotype (n=4). |
| data type |
Transcriptome or Gene Expression
|
| publication |
|
| properties ▽ |
{...}
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| dbXrefs |
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| distribution |
JSONJSON-LD
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| Download |
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| status |
public |
| visibility |
unrestricted-access |
| dateCreated |
2017-03-12T04:07:13+09:00 |
| dateModified |
2017-08-01T13:12:39+09:00 |
| datePublished |
2017-08-01T13:12:39+09:00 |