| identifier |
PRJDB6385 |
| type |
bioproject |
| sameAs |
|
| organism |
Mus musculus
|
| title |
DNMTs function as co-repressors in MAX-mediated repression of germ cell-related genes in mouse embryonic stem cells |
| description |
In embryonic stem cells (ESCs), the expression of development-related genes, including germ cell-related genes, is globally repressed. The transcription factor MAX represses germ cell-related gene expression in ESCs via PCGF6-polycomb repressive complex (PRC) 1, which consists of several epigenetic factors. However, we predicted that MAX represses germ cell-related gene expression through several additional mechanisms because PCGF6-PRC1 regulates the expression of only a subset of genes repressed by MAX. Here, we report that MAX associated with DNA methyltransferases (DNMTs) and cooperatively controlled germ cell-related gene expression in ESCs. To elucidate the significance of functional interactions between MAX and DNMTs on a genome-wide level, we performed targeted methylome sequencing (TMS) for semi-comprehensive DNA methylome analysis of Max-Knockdown ESCs and a differentially methylated region (DMR) calling identified 17 genes which had hypomethylated DMRs in the proximity of TSSs compared with control ESCs. Our findings provide insights into our understanding of MAX-based repressive mechanisms of germ cell-related genes in ESCs. |
| data type |
Epigenomics
|
| publication |
|
| properties ▽ |
{...}
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| dbXrefs |
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| distribution |
JSONJSON-LD
|
| Download |
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| status |
public |
| visibility |
unrestricted-access |
| dateCreated |
2017-09-20T03:26:11+09:00 |
| dateModified |
2018-11-21T13:04:32+09:00 |
| datePublished |
2018-11-21T13:04:32+09:00 |