home > bioproject > PRJDB6855
identifier PRJDB6855
type bioproject
sameAs
organism groundwater metagenome
title Microbial rapid denitrification system in Kathmandu, Nepal
description Microbial community structure changes of onsite groundwater treatment system was evaluated. Total DNA were extracted from samples 0.1 of g wet weight (Day 0 and 430) by using FastDNA SPIN Kit for the Soil (MP-Biomedicals). The V4 hyper variable region of the bacterial 16S rRNA gene was amplified by PCR using primers Eub-515f and Eub-806r. The PCR reaction comprised 20 cycles. Then, the amplified products were submitted to next-generation sequencing using an Illumina Miseq gene sequencer at a commercial sequencing service.
data type Metagenome
publication
properties 
{...}
dbXrefs
sra-run  DRR127198DRR127199
sra-submission  DRA006658DRA006659
biosample  SAMD00114507SAMD00114508
sra-study  DRP005947
sra-sample  DRS127921DRS127913
sra-experiment  DRX119942DRX119943
distribution JSONJSON-LD
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status public
visibility unrestricted-access
dateCreated 2018-03-20T08:31:01+09:00
dateModified 2020-03-20T13:09:07+09:00
datePublished 2020-03-20T13:09:07+09:00