| description |
We analyzed the effect of CtIP overexpression and CtIP accumulation at DSB site by LoAD system in terms of the accuracy and repair pathway choice. The short 3× Flag tag was knocked-in in this assay to enable sequence analysis covering the full length (left microhomology, knock-in fragment, and right microhomology) by next-generation sequencing (NGS). For the unbiased analysis of all analyzable alleles including precise knock-in, imprecise knock-in, and non-knock-in ones, we performed out-out PCR of genomic integration sites at the ATP5B, PARP1, and RPL11 loci, followed by NGS analysis. |