home > bioproject > PRJDB7349
identifier PRJDB7349
type bioproject
sameAs
organism Arabidopsis thaliana
title Genome-wide expression analysis regulated by internal nitrate in Arabidopsis thaliana
description The effects of internal nitrate level on transcriptome in Arabidopsis thaliana were analyzed. To manipulate internal nitrate levels independently of internal organic nitrogen, wild-type accession Col and a nitrate reductase (NR)-null mutant were used. Col and the NR-null mutant were grown for 7 days using 2.5 mM ammonium as the sole nitrogen source. The plants were treated with 10 mM nitrate and 2.5 mM ammonium for the first 24 hours so that they would accumulate nitrate. The plants were then transferred to nitrogen-free medium and grown for 5 days. At the time, nitrate concentrations in the shoots of NR-null mutant were significantly larger than those of Col, whilst, in the roots, no significant difference in nitrate concentration was detected between Col and the mutant. Using their shoots and roots, RNA sequencing was performed.
data type Transcriptome or Gene Expression
publication
properties 
{...}
dbXrefs
sra-run  DRR149027DRR149028DRR149029DRR149030DRR149031DRR149032DRR149033DRR149034
sra-submission  DRA007269
biosample  SAMD00135884SAMD00135885SAMD00135886SAMD00135887SAMD00135888SAMD00135889SAMD00135890SAMD00135891
sra-study  DRP005248
sra-sample  DRS101725DRS101726DRS101727DRS101728DRS101729DRS101730DRS101731DRS101732
sra-experiment  DRX139778DRX139779DRX139780DRX139781DRX139782DRX139783DRX139784DRX139785
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status public
visibility unrestricted-access
dateCreated 2018-08-24T00:52:34+09:00
dateModified 2019-07-08T13:56:35+09:00
datePublished 2019-07-08T13:56:34+09:00