| identifier |
PRJDB7465 |
| type |
bioproject |
| sameAs |
|
| organism |
Chikungunya virus
|
| title |
A field diagnosis and genotyping of chikungunya virus by a dried RT-LAMP and MinION sequencing |
| description |
Detection and sequencing of chikungunya virus (CHIKV) genome was performed using modified reverse transcription loop-mediated isothermal amplification (RT-LAMP) method and MinION sequencer. We developed the protocol for drying all the reagents for RT-LAMP in a single reaction tube with trehalose. The dried RT-LAMP tube is stable at room temperature, therefore it is not necessary to use cold-chain for providing the system to the remote area where chikungunya is endemic. Using the established system, CHIKV genome was effectively amplified under isothermal condition, and used as a template for MinION sequencing with a laptop computer. Our in-house RT-LAMP method and MinION sequencing system were validated with RNAs and serum samples from chikungunya patients in Brazil. The obtained sequence data was informative to recognize recent CHIKV outbreak genotype. |
| data type |
Targeted Locus (Loci)
|
| publication |
|
| properties ▽ |
{...}
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| dbXrefs |
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| distribution |
JSONJSON-LD
|
| Download |
|
| status |
public |
| visibility |
unrestricted-access |
| dateCreated |
2018-09-26T07:02:50+09:00 |
| dateModified |
2019-05-25T13:06:22+09:00 |
| datePublished |
2019-05-25T13:06:22+09:00 |