home > bioproject > PRJDB7701
identifier PRJDB7701
type bioproject
sameAs
organism Nasutitermes takasagoensis
title RNA sequencing of the heads of male castes in Nasutitermes takasagoensis by Illumina Hiseq
description For RNA-seq analysis, total RNA was extracted from the heads of 50 individuals each of soldier, presoldiers, minor workers and male alates. The heads of soldiers were divided into two parts: a frontal gland Class 1 cell layer and other tissues. As a comparison, we also examined the head tissues (including the frontal gland Class 1 cell layer) in another colony. RNA extraction was performed using an RNeasy mini Kit with DNase treatment. TruSeq RNA sample preparation kits (Version 2) were used to generate a complementary DNA library from 1 µg of total RNA from each caste or tissue. The cDNA libraries were individually indexed for multiplex sequencing before being pooled and sequenced on a HiSeq 2000 platform with 101 bp paired-end reading.
data type Transcriptome or Gene Expression
publication
31228516
properties 
{...}
dbXrefs
sra-run  DRR162556DRR162557DRR162558DRR162559DRR162560DRR162561
sra-submission  DRA007712
biosample  SAMD00152162SAMD00152163SAMD00152164SAMD00152165SAMD00152166SAMD00152167
sra-study  DRP005187
sra-sample  DRS100563DRS100564DRS100565DRS100566DRS100567DRS100568
sra-experiment  DRX153175DRX153176DRX153177DRX153178DRX153179DRX153180
distribution JSONJSON-LD
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status public
visibility unrestricted-access
dateCreated 2018-11-23T03:44:21+09:00
dateModified 2019-06-25T03:05:51+09:00
datePublished 2019-06-24T13:07:26+09:00