identifier |
PRJDB7701 |
type |
bioproject |
sameAs |
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organism |
Nasutitermes takasagoensis
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title |
RNA sequencing of the heads of male castes in Nasutitermes takasagoensis by Illumina Hiseq |
description |
For RNA-seq analysis, total RNA was extracted from the heads of 50 individuals each of soldier, presoldiers, minor workers and male alates. The heads of soldiers were divided into two parts: a frontal gland Class 1 cell layer and other tissues. As a comparison, we also examined the head tissues (including the frontal gland Class 1 cell layer) in another colony. RNA extraction was performed using an RNeasy mini Kit with DNase treatment. TruSeq RNA sample preparation kits (Version 2) were used to generate a complementary DNA library from 1 µg of total RNA from each caste or tissue. The cDNA libraries were individually indexed for multiplex sequencing before being pooled and sequenced on a HiSeq 2000 platform with 101 bp paired-end reading. |
data type |
Transcriptome or Gene Expression
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publication |
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properties ▽ |
{...}
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dbXrefs |
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distribution |
JSONJSON-LD
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Download |
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status |
public |
visibility |
unrestricted-access |
dateCreated |
2018-11-23T03:44:21+09:00 |
dateModified |
2019-06-25T03:05:51+09:00 |
datePublished |
2019-06-24T13:07:26+09:00 |