home > bioproject > PRJDB92
identifier PRJDB92
type bioproject
sameAs
organism Hodotermopsis sjostedti
title Differential gene expression in response to juvenile hormone analog treatment in the damp-wood termite Hodotermopsis sjostedti (Isoptera, Termopsidae)
description Normalized cDNA library was constructed from mRNA that was extracted from various castes/developmental stages of Hodotermopsis sjoestedti collected from Yaku-shima Island, Kagoshima, Japan. We employed 454 FLX Titanium system for sequencing the normalized cDNA library, assembled 454 reads with Newbler v2.5.3, and created a EST contig set. To identify genes that respond to a juvenile hormone analog (JHA) application in pseudergates of H. sjoestedti, we carried out JHA treatments on pseudoergates and Suppressive Subtractive Hybridization (SSH). We determined nucleic acid sequences of gene fragments generated from the SSH, and performed similarity searches for those fragments in the 454 EST contig set, using the blastx and tblastx algorithms. This BioProject entry includes the EST contigs that were obtained from the blast searches.
data type Transcriptome or Gene Expression
publication
24098800
properties 
{...}
dbXrefs
sra-run  DRR001939
sra-submission  DRA000538
biosample  SAMD00000639
sra-study  DRP000564
sra-sample  DRS001329
sra-experiment  DRX001400
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status public
visibility unrestricted-access
dateCreated 2012-04-23T02:03:18+0000
dateModified 2015-11-17T05:23:22+0000
datePublished 2013-03-29T00:49:27+0000