| description |
This study aims at the metagenomic analysis of ligninocellulose-degrading microbial communities of Mediterranean forest soils. For this purpose, two soil ecosystems with distinct ecophysiological characteristics were selected, and thorough spatio-seasonal sampling was undertaken. Total DNA was extracted from 48 soil samples, and PCR amplification was performed with both 16S (V3-V4) region- and ITS2-specific universal primers for the analysis of bacterial and fungal diversity, respectively. High-throughput amplicon sequencing was performed with the Illumina MiSeq technology. |