| identifier | PRJEB10543 |
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| type | bioproject |
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| title | Transcriptional_regulatory_network_during_ILC2_commitment |
| description | This study aims to investigate the molecular meachasim of ILC2 commitment and Bcl11b regulatory networks in ILC2 cells. The genome-wide transcriptomic heterogeneity in progenitors before ILC2 lineage commitemnt or after ILC2 lineage commitment and Bcl11b-deficient ILC2 progenitors will be studied by single-cell mRNA sequencing technology.Brief protocol:Adult mouse ILC progenitors harvested from femur and purified by FACS. Whole transcriptome amplification using Smartseq2 for full length transcripts in 96-well plates. Library is prepared by Illumina Nextera XT kit as pools of 96 samples.This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/ |
| data type | Other |
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