home > bioproject > PRJEB11277
identifier PRJEB11277
type bioproject
sameAs
organism
title RNA-seq Transcriptional Profiling of Herbaspirillum seropedicae Colonizing Bread Wheat (Triticum aestivum) Roots.
description H. seropedicae is a diazotrophic and endophytic bacterium that associates with economically important grasses promoting plant growth and increasing productivity. To identify genes related to bacterial ability to colonize and promote plant growth wheat seedlings growing hydroponically in Hoagland’s medium were inoculated with H. seropedicae the bacteria and incubated for 3 days. mRNA from the bacteria present in the root surface and in the plant medium were purified, depleted from rRNA and used for RNA-seq profiling. RT-qPCR analyses were conducted to confirm regulation of selected genes. Comparison of RNA profile of bacteria attached to the root and planktonic revealed an extensive metabolic adaptation to the epiphytic life style.
data type Transcriptome or Gene expression
organization
publication
properties 
{...}
dbXrefs
sra-run  ERR1049832ERR1049833ERR1049834ERR1049835ERR1049836ERR1049837ERR1049838ERR1049839ERR1049840ERR1049841
sra-submission  ERA520681
biosample  SAMEA3593399SAMEA3593400SAMEA3593401SAMEA3593402
sra-study  ERP012641
sra-sample  ERS900548ERS900549ERS900550ERS900551
sra-experiment  ERX1130123ERX1130124ERX1130125ERX1130126
distribution JSONJSON-LD
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bioproject.xml  HTTPS FTP
status public
visibility unrestricted-access
dateCreated 2016-01-02T00:00:00Z
dateModified 2016-01-02T00:00:00Z
datePublished 2016-01-01T00:00:00Z