| description |
In this study we have deleted four metabolic genes (HIS3, LEU2, URA3 and MET15) in their sixteen possible combinations. These strains are following: knockout of HIS3, LEU2, URA3, MET15; knockout of HIS3, LEU2, MET15; knockout of HIS3, URA3, MET15; knockout of LEU2, URA3, MET15; knockout of HIS3, LEU2, URA3; knockout of HIS3, MET15; knockout of LEU2, MET15; knockout of URA3, MET15; knockout of HIS3, LEU2; knockout of HIS3, URA3; knockout of LEU2, URA3; knockout of MET15, knockout of HIS3; knockout of LEU2; knockout of URA3 and prototrophic strain. We grew the 16 strains in synthetic complete media (SC), and sampled for transcriptomincs in triplicates at mid-exponential phase (OD value of 0.8). |