| identifier |
PRJEB11644 |
| type |
bioproject |
| sameAs |
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| organism |
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| title |
mouse Sperm DNA OxiDIP sequencing |
| description |
The transcriptionaly and translationally silent spermatozoa is devoid of any protection against oxidative DNA damage. It depends largely on its environment to protect it from reactive oxygen species (ROS)–mediated DNA alterations. The most common of these alterations is the oxidation of guanine residues into 8-OHdG that will have to be removed by the oocyte base excision repair (BER) pathway just after fertilization. To evaluate how sperm DNA oxidative damage may affect the paternal genome we carried out an oxidized DNA immunoprecipitation (OxiDIP) assay followed by a deep sequencing step of the precipitated fragments comparing sperm from WT mouse with sperm from a transgenic mouse in which mature sperm exhibit high post-testicular DNA oxidative damage (the Gpx5-/- mouse model [Chabory et al., The J. Clin. Invest., 2009]). Sequence analyses revealed that oxidized sequences are not uniformly distributed and some chromosomes appear more sensitive than others. This is not related to the chromosome richness in persisting nucleosomes but seems to be associated with the position of the chromosomes in the sperm nucleus. Oxidized sequences were found enriched in Short Interspersed Nuclear Elements (SINEs) and concern rather demethylated sperm intergenic DNA sequences. Overall design : Next Generation Sequencing of mouse sperm nuclear domains susceptible to oxidative alterations. |
| data type |
Other
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| organization |
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| publication |
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| properties ▽ |
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| dbXrefs |
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| distribution |
JSONJSON-LD
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| Download |
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| status |
public |
| visibility |
unrestricted-access |
| dateCreated |
2016-01-02T00:00:00Z |
| dateModified |
2016-01-02T00:00:00Z |
| datePublished |
2016-01-02T00:00:00Z |