identifier | PRJEB12121 |
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type | bioproject |
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title | Effect of hypoxia and atpenin A5 on the human monocyte transcriptome |
description | Monocyte-enriched peripheral blood mononuclear cells of apparently healthy human blood donors were cultured under normoxia or hypoxia (1% O2) for 24 hours at a high density (>20 million/ml). Normoxic cultures were also treated with 1 uM atpenin A5 for 48 hours. Afterwards, CD14+ monocytes were isolated using magnetic beads and their transcriptomes examined by sequencing rRNA-depleted total RNA (Illumina TruSeq Stranded Total RNA library preparation kit; HiSeq 2000 sequencer; paired 101 b reads; 6 libraries per flow lane). |
data type | Other |
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