| identifier | PRJEB12223 | ||
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| type | bioproject | ||
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| title | Transcriptome_analysis_of_mouse_pluripotency_genes | ||
| description | We aim to characterize transcriptional changes in mouse embryonic stem (ES) cells and mouse epiblast stem (EpiS) cells following the knockdown of essential genes identified in genome-wide RNAi screens. ES or EpiS cells were treated with esiRNA for 125 candidate genes and total RNA was collected from two biological replicates 72 hours post-transfection. The RNAs were normalized and arrayed in 96-well plates (500ng in 50 microlitres). Bar-coded libraries will be prepared from each 96-well plate and subjected to RNA-seq (Hiseq V4, 75 bp pair-end reads).This data is part of a pre-publication release. The data will be made available immediately via the ENA | ||
| data type | Transcriptome or Gene expression | ||
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| distribution | JSONJSON-LD | ||
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| status | public | ||
| visibility | unrestricted-access | ||
| dateCreated | 2016-02-09T00:00:00Z | ||
| dateModified | 2016-02-09T00:00:00Z | ||
| datePublished | 2016-02-09T00:00:00Z |