| description |
Temperature is a major factor determining the performance of the anaerobic digestion process. Themicrobial abundance, activity and interactional networks were investigated under a temperature gradient from 25 ?C to 55 ?C through amplicon sequencing, using 16S ribosomal RNA and 16S rRNA gene-basedapproaches. Comparative analysis of past accumulative elements presented by 16S rRNA gene-basedanalysis, and the in-situ conditions presented by 16S rRNA-based analysis, provided new insights concerning the identification of microbial functional roles and interactions. The daily methane productionand total biogas production increased with temperature up to 50 ?C, but decreased at 55 ?C. Increasedmethanogenesis and hydrolysis at 50 ?C were main factors causing higher methane production whichwas also closely related with more well-defined methanogenic and/or related modules with comprehensive interactions and increased functional orderliness referred to more microorganisms participating ininteractions. This research demonstrated the importance of evaluating functional roles and interactionsof microbial community. |