| description |
The aim of this project is to compare the structure of bacterial communities, created by using different DNA extraction protocols, from soil samples of the phosphogypsum waste heap in Wislinka, (Poland, Pomeranian district). The selection of proper methods is of a high importance in every research, due to its influence on the obtained results and theirs interpretation. Therefore, in this project we want to verify, whether the DNA extraction methods have a significant impact on the proper recognition of the final structure of bacterial communities. During this study, we want to determine whether the results are strongly influenced by choice of DNA extraction protocols or significant changes in experimentally estimated structure of community cannot be observed. Several evaluation studies have been performed in order to compare DNA extraction methods from soil samples. However, these studies have never compared the diversity of obtained sequences by high-throughput sequencing. In this project we want to compare not only yield and purity of extracted DNA using different isolation protocols, but also the results of the sequencing, and their impact on recognized complexity of microbial community structure estimated on their basis. In this study we collected soil samples from the phosphogypsum waste heap in Wislinka, (Pomerania district). The heap was created from production wastes, mainly phosphogypsum and due to that fact it may be attractive place to study biodiversity of microorganisms. This study can be a valuable source of rare and unique types of bacteria living in very unique environmental conditions. |