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Total RNA was isolated from the head kidney/spleen of six healthy juvenile Atlantic cod. These fish originate from the Norwegian cod breeding program and were grown out in in seawater of 3. 4 % salinity at 10 ºC, 24 hour light and fed ad libitum with commercial feed at the Aquaculture Research Station (Tromsø, Norway). The fish were reported to be healthy without any history of diseases and the use of these fish was approved by the National Animal Research authority in Norway (FOTS id 1147). The tissue was stored on RNAlater (Life Technologies) and total RNA was isolated using Trizol (Life Technologies) according to protocol but using half the amount of tissue per volume Trizol recommended by the manufacturer. Sequencing libraries were produced according to the IlluminaTruSeq protocol (Illumina, Inc., San Diego, CA). Illumina HiSeq2000 100 bp paired-end sequencing services were provided by the Norwegian Sequencing Centre (http://www. sequencing.uio. no). |