| description |
Purified and semi-purified polysaccharides characteristic of plant cell wall components of cereals and fruits, together with meat protein, were fermented in vitro with a pig faecal inoculum, using the cumulative gas production technique, to examine the kinetics and end-products of fermentation after 48 h. The microbial assemblage at T1/2 and 2xT1/2 for each substrate was sequenced and characterised using 454 amplicon pyrosequencing. Results obtained suggested that mango pulp and pectin were rapidly fermented, whilst the arabinoxylan enriched fraction (AXRF), arabinoxylan (AX), mixed linkage glucan (MLG), and a mixture of these plant cell wall polysaccharides (AXMLG) were more slowly fermented and to a lesser extent. Cellulose and meat protein underwent minimal fermentation. In addition, a statistical comparison of in vitro results with previous in vivo data indicated that the substrate or diet appeared to drive the end-product proportions whatever the method, and despite the markedly different methodologies. Furthermore, it is shown that certain bacterial Phyla are correlated with the substrates fermented. |