| description |
We have recently isolated a heavily capsulated bacterium that can kill and degrade the nematode C.elegans, that we would like to get sequenced in order to understand its mechanisms of action on the worm. Its mode of action seems to be by directly digesting the nematodes Collagen and this process continues after the worm has been killed by the bacterium. It was isolated from France from nematodes in the wild. Initially it was thought to belong to the Species Elizabethkingia (a Flavobacteria) but the 16s rRNA seq indicates that it is closer to Chryseobacterium sp. - also a Flavobacteria. We have managed to isolate it from the lab E. coli that is used to feed the worms. It grows at 20oC but not 37oC to produce brown pigmented mucoid colonies. It would be really useful to have genome sequence with as large contigs as possible, hence PacBio. Chryseobacterium sp may provide a wealth of anti-microbials that require further characterisation. For example some isolates found in the soil degrade N-acylhomoserine lactones (AHLs) which act as signal compounds involved in quorum sensing for many pathogens including those of plants. Others degrade keratinous waste so may find a use in waste disposal sites while other uses await discovery.This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute please see http://www.sanger.ac.uk/datasharing/ |