| description |
In this project, using T. muris, a mouse model of T. trichuira infection in humans, we study the role of the receptors of IL-10 superfamily members on epithelial cells during whipworm infection and immunity. Thus, mutant mouse lines for interleukin 10 (IL-10) receptor beta, IL-10 receptor alpha and IL-10 are challenged with T. muris and the influence of these mutations on anti-parasite immunity and expulsion is evaluated. High dose infection with T. muris results in IL-10 receptor beta, IL-10 receptor alpha and IL-10 mutant mice succumbing to whipworm infection at day 21 post infection. Interestingly, we found the susceptibility of IL-10 receptor beta mutant mice to T. muris is related to a leaky gut syndrome that allows the colonization of the liver by members of the gut microbiota. To identify the members of the microbiota that colonize the liver, we propose to perform 16S sequencing of the liver and caecal content of infected animals.This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/ |