| identifier | PRJEB14790 | ||||||||||||
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| type | bioproject | ||||||||||||
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| title | iCLIP of TDP-43 | ||||||||||||
| description | A stable HEK293 FlpIn T-Rex cells expressing TDP-43 with an N-terminal eGFP-tag was generated that allowed inducible physiological expression of the protein (Ling et al. 2010). Duplicate iCLIP experiments were performed using an antibody targeting eGFP (Abcam ab290). Crosslinked RNA-protein complexes were isolated by immuno-precipitation and cDNAs were generated to allow preparation of Illumina compatible DNA libraries as described in Huppertz et al. (2014). | ||||||||||||
| data type | Other | ||||||||||||
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| distribution | JSONJSON-LD | ||||||||||||
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| status | public | ||||||||||||
| visibility | unrestricted-access | ||||||||||||
| dateCreated | 2017-05-02T00:00:00Z | ||||||||||||
| dateModified | 2017-05-02T00:00:00Z | ||||||||||||
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