| identifier | PRJEB14963 |
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| type | bioproject |
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| title | Technical biases and variations on species abundances for a repetitively sequenced fecal sample 16S rRNA library. |
| description | The high throughput sequencing powered by next generation sequencing offered unprecedented resolution in delineating the composition of microbial profiles. To best evaluate the biases and artifacts entangled within the sequencing output we had repeatedly sequenced an amplicon library from the same fecal sample 10 times in 4 distinct Illumina MiSeq sequencing runs. Analysis of the observed Operational Taxonomic Units (OTUs) showed as expected a strong correlation of relative abundance with the coefficient of variation. The results are used to argue about the appropriate relative abundance exclusion cutoff used in the Rhea pipeline for the analysis of microbial profiles. |
| data type | Other |
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