description |
The fungal populations present in Vitis vinifera L. cv. Corvina grapes and musts were compared in two different years vintages. The comparison was carried out through ITS1-5.8S-ITS2 454-pyrosequencing. Grapes were collected from a recent vineyard located in the Italian winery region called "Valpolicella" (Sant'Ambrogio, Verona, Italy). After the harvesting, grapes were subjected to withering in a dedicated warehouse located a few kilometers far from the vineyars (<5Km). The warehouse is supplemented with automatic systems able to control and modify the internal temperature and humidity. The duration of grape withering was defined according to the regional rules for Amarone production (Paronetto and Dellaglio, 2011), which define the time in which the grapes for Amarone vinification can be mashed. The comparison of the fungal populations present in these samples sheds lights on the Amarone production, allowing the identification of the persistent fungal genera and on genera varying as suffering from seasonal environmental changes. |