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The objective of the project is to address the lack of knowledge of the diversity of adaptive stress responses, such as intra- and inter-species communication, population-level cooperation, and the principles underlying signal transduction and information processing. To examine these phenomenon the psychrophilic fish pathogens Moritella viscosa and Vibrio wodanis (Winter ulcers) will be studied. By using an whole transcriptome sequence (RNA-Seq) analysis approach in combination with comparative genomics, the main objective will be achieved by: i) in vitro cultivation studies under various environmental stress conditions and ii) using implant (in vivo) to mimic physiological stress condition under infection. In vivo, Moritella viscosa and Vibrio wodanis were grown both in separate implants in the abdomen of the fish and together in the same implant. In vitro, Moritella viscosa and Vibrio wodanis were both co-cultivated in the same culture and cultivated separately. This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/ |